Rmu_sc0004145.1_g000017

Tumour-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004145.1
OG0008622
Physical Location & Seq
Forward (+)
122202 .. 131920
9719 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1605 bp
atggatccggagcagacattcatacgcgtacaagagcgcttctctcagatcctgacgccaaaagtgagagctgctttggagtatatctaccttttcatcgcgatcacactcttctgtattctcgttgttatgcacgctaattacgttcagcagcctggctgttcaagtgagcttcctggagttgaaacagcagacgcagaaattattcatgttaagatttccagtgcaggcttgtggtcgcagaatgagtcagaattcaccaatgtgataaatgatgtccccgagacgaaatttgtggtggataaattagaatttgccaatgtaaatggggatgagattctggatcatcaggcagatagctctatgccaaatgttgatgatgcggttatgaaaactgaaatagaggagtcacgtagtagttttcctttatctgcaaaagagacattcaaagcagcactctttcattttagcaacaagtggcacaggcgtctatcattcctttggaagcatggaacacatattgttggaagtttgtggaacattgcaggcatacatttggatcttgatattccaaagtggctgcatatacttcgattggacaggcttaactcttatgcagtgcagtggcttgagaacagaagcaaaacttttgaaccaacttacttatacactatggaaaagggttatttcttactgcctgaaagtgcaagagctcagcataatattcgtactgtaaacataagcatatcagctcgacattcctgtttcggaaacaggtggcagcagctactgattaacagatttgttgggtacgatactattttgataaacagtttactgagttctcctggtcaaggttatctttataattttcaaacaaaggagttctttaatcttagttatgcacatgaaccgccagagggtccggcaagatttggagactacctcgtgacaaagtgtggcgttcttatgatgtctctatttgtgttctttacaaccactatgtctgtgtcatttactttgagagagacgcagactcggatgctgaagttcacagtgcagcttcaacaccatgctcgtcatcagcttccaacatttcagttgatttttgtgcatgtaattgaatcacttgtctttgtcccaattatgattggcatattgttttttctgttcgagttttacgatgatcaactattggccttcatggtcctaattcttgtctggttgagtgaactatttaccctgatcagtgttcgcacgcccatatcaatgaagttctttccccgcttctttctgctctactttctggtttttcacatctatttcttttcctatgcttacggtttttcctatttggctctttctacggccgcagcatttatgcagcatctcattctatacttctggaaccgttttgaggttgcagatggcgactttgatgttgatttcctgactagacggagtctgaaatcaactgtcacagatggtgacagatcacagatggtgatcaaggcaggaataggtaatcacgtcctggggctaatcctgcaggtcaggaaaatcgtggctgaagctgaaggagcttgttggcagcagaacgggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Membralin

Protein Analysis

534

Amino Acids

61.77

Weight (kDa)

6.26

Isoelectric Point (pI)

49.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Membralin PF09746 7 - 424 7.9e-24 Tumour-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 867
Acc36I ACCTGC 1 cut(s) 1540
AccII CGCG 2 cut(s) 27, 101
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 4 cut(s) 383, 914, 1285, 1371
AclWI GGATC 4 cut(s) 12, 43, 351, 567
AcoI YGGCCR 1 cut(s) 1368
AcsI RAATTY 3 cut(s) 254, 290, 311
AcuI CTGAAG 3 cut(s) 1067, 1590, 1596
AcyI GRCGYC 2 cut(s) 56, 487
AfaI GTAC 3 cut(s) 30, 730, 812
AfeI AGCGCT 1 cut(s) 38
AfiI CCNNNNNNNGG 3 cut(s) 854, 920, 1417
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 7 cut(s) 165, 185, 448, 653, 875, 1067, 1124
AjiI CACGTC 1 cut(s) 1531
AjnI CCWGG 4 cut(s) 154, 175, 847, 1533
AleI CACNNNNGTG 1 cut(s) 263
Alw21I GWGCWC 1 cut(s) 715
Alw26I GTCTC 5 cut(s) 278, 434, 933, 981, 1022
AlwI GGATC 4 cut(s) 12, 43, 351, 567
AlwNI CAGNNNCTG 2 cut(s) 52, 790
Ama87I CYCGRG 1 cut(s) 281
Aor13HI TCCGGA 1 cut(s) 7
Aor51HI AGCGCT 1 cut(s) 38
AoxI GGCC 2 cut(s) 1197, 1368
ApoI RAATTY 3 cut(s) 254, 290, 311
Asp700I GAANNNNTTC 1 cut(s) 204
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 2 cut(s) 923, 1207
AsuHPI GGTGA 3 cut(s) 250, 1499, 1516
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 2 cut(s) 923, 1207
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 715
BauI CACGAG 1 cut(s) 947
Bbv12I GWGCWC 1 cut(s) 715
BbvI GCAGC 9 cut(s) 58, 163, 464, 567, 793, 796, 1072, 1385, 1396
BccI CCATC 3 cut(s) 1421, 1478, 1495
BceAI ACGGC 1 cut(s) 1383
BcgI CGANNNNNNTGC 4 cut(s) 572, 606, 707, 741
BciT130I CCWGG 4 cut(s) 156, 177, 849, 1535
BclI TGATCA 3 cut(s) 1186, 1245, 1506
BcoDI GTCTC 5 cut(s) 278, 434, 933, 981, 1022
BfaI CTAG 1 cut(s) 1455
BfmI CTRYAG 1 cut(s) 1547
BfoI RGCGCY 1 cut(s) 40
BfuAI ACCTGC 1 cut(s) 1540
BlpI GCTNAGC 1 cut(s) 714
Bme1390I CCNGG 4 cut(s) 156, 177, 849, 1535
Bme18I GGWCC 2 cut(s) 923, 1207
BmeT110I CYCGRG 1 cut(s) 281
BmgBI CACGTC 1 cut(s) 1531
BmgT120I GGNCC 2 cut(s) 923, 1207
BmiI GGNNCC 3 cut(s) 6, 924, 1409
BmrFI CCNGG 4 cut(s) 156, 177, 849, 1535
BmsI GCATC 3 cut(s) 370, 1032, 1396
BplI GAGNNNNNCTC 4 cut(s) 26, 58, 930, 962
BpmI CTGGAG 1 cut(s) 198
Bpu1102I GCTNAGC 1 cut(s) 714
BpuEI CTTGAG 1 cut(s) 650
BsaAI YACGTR 1 cut(s) 413
BsaBI GATNNNNATC 1 cut(s) 1505
BsaHI GRCGYC 2 cut(s) 56, 487
BsaJI CCNNGG 1 cut(s) 1534
BsaWI WCCGGW 1 cut(s) 7
Bsc4I CCNNNNNNNGG 3 cut(s) 854, 920, 1417
Bse1I ACTGG 1 cut(s) 222
Bse3DI GCAATG 1 cut(s) 540
Bse8I GATNNNNATC 1 cut(s) 1505
BseAI TCCGGA 1 cut(s) 7
BseBI CCWGG 4 cut(s) 156, 177, 849, 1535
BseDI CCNNGG 1 cut(s) 1534
BseGI GGATG 2 cut(s) 337, 1047
BseJI GATNNNNATC 1 cut(s) 1505
BseLI CCNNNNNNNGG 3 cut(s) 854, 920, 1417
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 3 cut(s) 59, 728, 830
BseNI ACTGG 1 cut(s) 222
BseRI GAGGAG 1 cut(s) 419
BseX3I CGGCCG 1 cut(s) 1368
BseXI GCAGC 9 cut(s) 58, 163, 464, 567, 793, 796, 1072, 1385, 1396
BsgI GTGCAG 3 cut(s) 246, 641, 1079
Bsh1236I CGCG 2 cut(s) 27, 101
Bsh1285I CGRYCG 1 cut(s) 1371
BshFI GGCC 2 cut(s) 1199, 1370
BsiEI CGRYCG 1 cut(s) 1371
BsiHKAI GWGCWC 1 cut(s) 715
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 2 cut(s) 8, 926
BslFI GGGAC 2 cut(s) 263, 1124
BslI CCNNNNNNNGG 3 cut(s) 854, 920, 1417
BsmAI GTCTC 5 cut(s) 278, 434, 933, 981, 1022
BsmBI CGTCTC 2 cut(s) 278, 1022
BsmFI GGGAC 2 cut(s) 263, 1124
BsnI GGCC 2 cut(s) 1199, 1370
BsoBI CYCGRG 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 715
Bsp13I TCCGGA 1 cut(s) 7
Bsp143I GATC 9 cut(s) 4, 48, 102, 343, 559, 1186, 1245, 1493, 1506
Bsp1720I GCTNAGC 1 cut(s) 714
Bsp68I TCGCGA 1 cut(s) 101
BspACI CCGC 4 cut(s) 383, 914, 1285, 1371
BspANI GGCC 2 cut(s) 1199, 1370
BspCNI CTCAG 3 cut(s) 58, 727, 831
BspEI TCCGGA 1 cut(s) 7
BspFNI CGCG 2 cut(s) 27, 101
BspLI GGNNCC 3 cut(s) 6, 924, 1409
BspMAI CTGCAG 1 cut(s) 1551
BspMI ACCTGC 1 cut(s) 1540
BspPI GGATC 4 cut(s) 12, 43, 351, 567
BsrDI GCAATG 1 cut(s) 540
BsrI ACTGG 1 cut(s) 222
BssECI CCNNGG 1 cut(s) 1534
BssMI GATC 9 cut(s) 4, 48, 102, 343, 559, 1186, 1245, 1493, 1506
BssNI GRCGYC 2 cut(s) 56, 487
BssSI CACGAG 1 cut(s) 947
Bst2BI CACGAG 1 cut(s) 947
Bst2UI CCWGG 4 cut(s) 156, 177, 849, 1535
Bst4CI ACNGT 6 cut(s) 733, 833, 1057, 1343, 1412, 1477
Bst6I CTCTTC 1 cut(s) 116
BstACI GRCGYC 2 cut(s) 56, 487
BstBAI YACGTR 1 cut(s) 413
BstC8I GCNNGC 4 cut(s) 135, 229, 547, 1259
BstDEI CTNAG 4 cut(s) 45, 714, 839, 896
BstENI CCTNNNNNAGG 1 cut(s) 852
BstF5I GGATG 2 cut(s) 337, 1047
BstFNI CGCG 2 cut(s) 27, 101
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 9 cut(s) 7, 51, 105, 346, 562, 1189, 1248, 1496, 1509
BstMAI GTCTC 5 cut(s) 278, 434, 933, 981, 1022
BstMBI GATC 9 cut(s) 4, 48, 102, 343, 559, 1186, 1245, 1493, 1506
BstMCI CGRYCG 1 cut(s) 1371
BstMWI GCNNNNNNNGC 2 cut(s) 1546, 1580
BstNI CCWGG 4 cut(s) 156, 177, 849, 1535
BstNSI RCATGY 1 cut(s) 1118
BstSCI CCNGG 4 cut(s) 154, 175, 847, 1533
BstSFI CTRYAG 1 cut(s) 1547
BstUI CGCG 2 cut(s) 27, 101
BstV1I GCAGC 9 cut(s) 58, 163, 464, 567, 793, 796, 1072, 1385, 1396
BstX2I RGATCY 3 cut(s) 4, 48, 559
BstYI RGATCY 3 cut(s) 4, 48, 559
BstZI CGGCCG 1 cut(s) 1368
BsuRI GGCC 2 cut(s) 1199, 1370
BtgZI GCGATG 1 cut(s) 82
BtrI CACGTC 1 cut(s) 1531
BtsCI GGATG 2 cut(s) 337, 1047
BtsI GCAGTG 2 cut(s) 624, 629
BtsIMutI CAGTG 5 cut(s) 229, 624, 629, 1062, 1255
BtuMI TCGCGA 1 cut(s) 101
BveI ACCTGC 1 cut(s) 1540
Cac8I GCNNGC 4 cut(s) 135, 229, 547, 1259
CaiI CAGNNNCTG 2 cut(s) 52, 790
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 2 cut(s) 923, 1207
CseI GACGC 4 cut(s) 64, 203, 476, 1039
Csp6I GTAC 3 cut(s) 29, 729, 811
CviAII CATG 6 cut(s) 209, 509, 908, 1073, 1115, 1204
CviQI GTAC 3 cut(s) 29, 729, 811
DdeI CTNAG 4 cut(s) 45, 714, 839, 896
DpnI GATC 9 cut(s) 6, 50, 104, 345, 561, 1188, 1247, 1495, 1508
DpnII GATC 9 cut(s) 4, 48, 102, 343, 559, 1186, 1245, 1493, 1506
EaeI YGGCCR 1 cut(s) 1368
EagI CGGCCG 1 cut(s) 1368
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Ecl136II GAGCTC 1 cut(s) 713
EclXI CGGCCG 1 cut(s) 1368
Eco24I GRGCYC 1 cut(s) 715
Eco47I GGWCC 2 cut(s) 923, 1207
Eco47III AGCGCT 1 cut(s) 38
Eco52I CGGCCG 1 cut(s) 1368
Eco53kI GAGCTC 1 cut(s) 713
Eco57I CTGAAG 3 cut(s) 1067, 1590, 1596
Eco88I CYCGRG 1 cut(s) 281
EcoICRI GAGCTC 1 cut(s) 713
EcoNI CCTNNNNNAGG 1 cut(s) 852
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 4 cut(s) 154, 175, 847, 1533
EcoT38I GRGCYC 1 cut(s) 715
Esp3I CGTCTC 2 cut(s) 278, 1022
FaeI CATG 6 cut(s) 212, 512, 911, 1076, 1118, 1207
FalI AAGNNNNNCTT 6 cut(s) 631, 663, 846, 878, 872, 904
FaqI GGGAC 2 cut(s) 263, 1124
FatI CATG 6 cut(s) 208, 508, 907, 1072, 1114, 1203
FauI CCCGC 1 cut(s) 1292
FbaI TGATCA 3 cut(s) 1186, 1245, 1506
FokI GGATG 2 cut(s) 344, 1054
FriOI GRGCYC 1 cut(s) 715
FspBI CTAG 1 cut(s) 1455
GlaI GCGC 1 cut(s) 38
GsuI CTGGAG 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 2 cut(s) 1199, 1370
HapII CCGG 2 cut(s) 8, 926
HgaI GACGC 4 cut(s) 64, 203, 476, 1039
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 2 cut(s) 56, 487
Hin1II CATG 6 cut(s) 212, 512, 911, 1076, 1118, 1207
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HinfI GANTC 6 cut(s) 248, 337, 407, 1036, 1124, 1462
HpaII CCGG 2 cut(s) 8, 926
HphI GGTGA 3 cut(s) 250, 1499, 1516
Hpy166II GTNNAC 4 cut(s) 736, 836, 1053, 1232
Hpy188I TCNGA 5 cut(s) 48, 253, 770, 1041, 1467
Hpy188III TCNNGA 9 cut(s) 8, 52, 100, 341, 563, 949, 1405, 1450, 1555
Hpy8I GTNNAC 4 cut(s) 736, 836, 1053, 1232
HpyAV CCTTC 2 cut(s) 1210, 1571
HpyCH4III ACNGT 6 cut(s) 733, 833, 1057, 1343, 1412, 1477
HpyCH4IV ACGT 3 cut(s) 144, 412, 1530
HpyF10VI GCNNNNNNNGC 2 cut(s) 1546, 1580
HpyF3I CTNAG 4 cut(s) 45, 714, 839, 896
HpySE526I ACGT 3 cut(s) 144, 412, 1530
Hsp92I GRCGYC 2 cut(s) 56, 487
Hsp92II CATG 6 cut(s) 212, 512, 911, 1076, 1118, 1207
HspAI GCGC 1 cut(s) 37
Kpn2I TCCGGA 1 cut(s) 7
Ksp22I TGATCA 3 cut(s) 1186, 1245, 1506
Kzo9I GATC 9 cut(s) 4, 48, 102, 343, 559, 1186, 1245, 1493, 1506
LmnI GCTCC 2 cut(s) 10, 1580
Lsp1109I GCAGC 9 cut(s) 58, 163, 464, 567, 793, 796, 1072, 1385, 1396
LweI GCATC 3 cut(s) 370, 1032, 1396
MaeI CTAG 1 cut(s) 1455
MaeII ACGT 3 cut(s) 144, 412, 1530
MaeIII GTNAC 4 cut(s) 408, 949, 1477, 1487
MalI GATC 9 cut(s) 6, 50, 104, 345, 561, 1188, 1247, 1495, 1508
MboI GATC 9 cut(s) 4, 48, 102, 343, 559, 1186, 1245, 1493, 1506
MboII GAAGA 1 cut(s) 103
MflI RGATCY 3 cut(s) 4, 48, 559
MhlI GDGCHC 1 cut(s) 715
MluI ACGCGT 1 cut(s) 25
MlyI GAGTC 4 cut(s) 257, 416, 1030, 1471
MmeI TCCRAC 2 cut(s) 505, 1115
MnlI CCTC 4 cut(s) 397, 913, 956, 1411
MroI TCCGGA 1 cut(s) 7
MroXI GAANNNNTTC 1 cut(s) 204
MseI TTAA 4 cut(s) 213, 606, 795, 891
MslI CAYNNNNRTG 2 cut(s) 263, 1268
MspI CCGG 2 cut(s) 8, 926
MspR9I CCNGG 4 cut(s) 156, 177, 849, 1535
MvaI CCWGG 4 cut(s) 156, 177, 849, 1535
MvnI CGCG 2 cut(s) 27, 101
MwoI GCNNNNNNNGC 2 cut(s) 1546, 1580
NdeII GATC 9 cut(s) 4, 48, 102, 343, 559, 1186, 1245, 1493, 1506
NlaIII CATG 6 cut(s) 212, 512, 911, 1076, 1118, 1207
NlaIV GGNNCC 3 cut(s) 6, 924, 1409
NmuCI GTSAC 4 cut(s) 408, 949, 1477, 1487
NruI TCGCGA 1 cut(s) 101
NspI RCATGY 1 cut(s) 1118
OliI CACNNNNGTG 1 cut(s) 263
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 1179
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 2 cut(s) 337, 1124
PflFI GACNNNGTC 1 cut(s) 1461
PfoI TCCNGGA 1 cut(s) 175
PleI GAGTC 4 cut(s) 256, 415, 1030, 1470
PpsI GAGTC 4 cut(s) 256, 415, 1030, 1470
Ppu21I YACGTR 1 cut(s) 413
PsiI TTATAA 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 715
Psp6I CCWGG 4 cut(s) 154, 175, 847, 1533
PspGI CCWGG 4 cut(s) 154, 175, 847, 1533
PspN4I GGNNCC 3 cut(s) 6, 924, 1409
PspPI GGNCC 2 cut(s) 923, 1207
PstI CTGCAG 1 cut(s) 1551
PstNI CAGNNNCTG 2 cut(s) 52, 790
PsuI RGATCY 3 cut(s) 4, 48, 559
PsyI GACNNNGTC 1 cut(s) 1461
RruI TCGCGA 1 cut(s) 101
RsaI GTAC 3 cut(s) 30, 730, 812
RsaNI GTAC 3 cut(s) 29, 729, 811
RseI CAYNNNNRTG 2 cut(s) 263, 1268
SacI GAGCTC 1 cut(s) 715
SaqAI TTAA 4 cut(s) 213, 606, 795, 891
Sau3AI GATC 9 cut(s) 4, 48, 102, 343, 559, 1186, 1245, 1493, 1506
Sau96I GGNCC 2 cut(s) 923, 1207
SbfI CCTGCAGG 1 cut(s) 1551
SchI GAGTC 4 cut(s) 257, 416, 1030, 1471
ScrFI CCNGG 4 cut(s) 156, 177, 849, 1535
SdaI CCTGCAGG 1 cut(s) 1551
SduI GDGCHC 1 cut(s) 715
SfaNI GCATC 3 cut(s) 370, 1032, 1396
SfcI CTRYAG 1 cut(s) 1547
SinI GGWCC 2 cut(s) 923, 1207
SmiMI CAYNNNNRTG 2 cut(s) 263, 1268
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse8387I CCTGCAGG 1 cut(s) 1551
SsiI CCGC 4 cut(s) 383, 914, 1285, 1371
SspI AATATT 1 cut(s) 724
SspMI CTAG 1 cut(s) 1455
SstI GAGCTC 1 cut(s) 715
StyD4I CCNGG 4 cut(s) 154, 175, 847, 1533
TaaI ACNGT 6 cut(s) 733, 833, 1057, 1343, 1412, 1477
TaiI ACGT 3 cut(s) 147, 415, 1533
TaqI TCGA 3 cut(s) 592, 754, 1173
TauI GCSGC 1 cut(s) 1373
TfiI GAWTC 2 cut(s) 337, 1124
Tru1I TTAA 4 cut(s) 213, 606, 795, 891
Tru9I TTAA 4 cut(s) 213, 606, 795, 891
TscAI CASTG 5 cut(s) 229, 624, 629, 1062, 1255
TseFI GTSAC 4 cut(s) 408, 949, 1477, 1487
Tsp45I GTSAC 4 cut(s) 408, 949, 1477, 1487
TspDTI ATGAA 8 cut(s) 10, 85, 197, 404, 452, 924, 1192, 1286
TspGWI ACGGA 1 cut(s) 1474
TspRI CASTG 5 cut(s) 229, 624, 629, 1062, 1255
Tth111I GACNNNGTC 1 cut(s) 1461
VpaK11BI GGWCC 2 cut(s) 923, 1207
XagI CCTNNNNNAGG 1 cut(s) 852
XapI RAATTY 3 cut(s) 254, 290, 311
XceI RCATGY 1 cut(s) 1118
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 1 cut(s) 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.