Rmu_sc0004413.1_g000031

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004413.1
OG0016467
Physical Location & Seq
Forward (+)
150489 .. 150857
369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004413.1_g000031.1.cds

Sequence Viewer

Length: 369 bp
atggggatccgaaccaggctagaagatcttttggatgttccaatttccgacgcaattcaggttgagaagaagggtctgatggaaaggcttcagttgcttctatcacaggaggaatccttctggagacaacgatctaaaataacctggctgagggagggggatagaaacaccgggtacttccatagaaaggtagctaaccggaaacggaaaaatatgataaaaggattgtatgatgaaaatgggatttggtgtgaggaggataaagatctggaacgagtggttaactcttacttcactactatgtttactgcatctaatattgatctcgatgcagtggagtcaactttggcagcaatatcacctgtgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

122

Amino Acids

14.25

Weight (kDa)

5.89

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 14
AcuI CTGAAG 1 cut(s) 74
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 2 cut(s) 150, 187
AjnI CCWGG 2 cut(s) 14, 143
AluBI AGCT 1 cut(s) 194
AluI AGCT 1 cut(s) 194
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 14
ApeKI GCWGC 1 cut(s) 350
Asp700I GAANNNNTTC 1 cut(s) 87
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 1 cut(s) 351
BamHI GGATCC 1 cut(s) 6
BbvCI CCTCAGC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 362
BccI CCATC 1 cut(s) 73
BciT130I CCWGG 2 cut(s) 16, 145
BcnI CCSGG 1 cut(s) 172
BcoDI GTCTC 1 cut(s) 118
BfaI CTAG 1 cut(s) 20
BglII AGATCT 2 cut(s) 25, 265
BisI GCNGC 1 cut(s) 351
BlsI GCNGC 1 cut(s) 352
Bme1390I CCNGG 3 cut(s) 16, 145, 172
BmiI GGNNCC 1 cut(s) 8
BmrFI CCNGG 3 cut(s) 16, 145, 172
BmsI GCATC 2 cut(s) 319, 320
BpmI CTGGAG 1 cut(s) 142
Bpu10I CCTNAGC 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 172
BsaBI GATNNNNATC 1 cut(s) 264
BsaWI WCCGGW 1 cut(s) 198
Bsc4I CCNNNNNNNGG 2 cut(s) 150, 187
Bse8I GATNNNNATC 1 cut(s) 264
BseBI CCWGG 2 cut(s) 16, 145
BseGI GGATG 1 cut(s) 40
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 2 cut(s) 150, 187
BseMII CTCAG 1 cut(s) 140
BseRI GAGGAG 1 cut(s) 269
BseXI GCAGC 1 cut(s) 362
BsiSI CCGG 2 cut(s) 171, 199
BslI CCNNNNNNNGG 2 cut(s) 150, 187
BsmAI GTCTC 1 cut(s) 118
Bsp143I GATC 5 cut(s) 6, 25, 131, 265, 322
BspCNI CTCAG 1 cut(s) 141
BspLI GGNNCC 1 cut(s) 8
BspPI GGATC 1 cut(s) 14
BssMI GATC 5 cut(s) 6, 25, 131, 265, 322
Bst2UI CCWGG 2 cut(s) 16, 145
BstDEI CTNAG 1 cut(s) 149
BstENI CCTNNNNNAGG 1 cut(s) 148
BstF5I GGATG 1 cut(s) 40
BstKTI GATC 5 cut(s) 9, 28, 134, 268, 325
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 5 cut(s) 6, 25, 131, 265, 322
BstMWI GCNNNNNNNGC 1 cut(s) 94
BstNI CCWGG 2 cut(s) 16, 145
BstSCI CCNGG 3 cut(s) 14, 143, 170
BstV1I GCAGC 1 cut(s) 362
BstX2I RGATCY 3 cut(s) 6, 25, 265
BstYI RGATCY 3 cut(s) 6, 25, 265
BtsCI GGATG 1 cut(s) 40
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 1 cut(s) 339
CseI GACGC 1 cut(s) 59
Csp6I GTAC 1 cut(s) 175
CviJI RGCY 4 cut(s) 19, 88, 148, 194
CviKI_1 RGCY 4 cut(s) 19, 88, 148, 194
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 1 cut(s) 149
DpnI GATC 5 cut(s) 8, 27, 133, 267, 324
DpnII GATC 5 cut(s) 6, 25, 131, 265, 322
Eco57I CTGAAG 1 cut(s) 74
EcoNI CCTNNNNNAGG 1 cut(s) 148
EcoRII CCWGG 2 cut(s) 14, 143
FaiI YATR 4 cut(s) 183, 215, 231, 302
Fnu4HI GCNGC 1 cut(s) 351
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 1 cut(s) 351
FspBI CTAG 1 cut(s) 20
GluI GCNGC 1 cut(s) 351
GsuI CTGGAG 1 cut(s) 142
HapII CCGG 2 cut(s) 171, 199
HgaI GACGC 1 cut(s) 59
HincII GTYRAC 2 cut(s) 283, 342
HindII GTYRAC 2 cut(s) 283, 342
HinfI GANTC 2 cut(s) 113, 338
HpaI GTTAAC 1 cut(s) 283
HpaII CCGG 2 cut(s) 171, 199
HphI GGTGA 1 cut(s) 351
Hpy166II GTNNAC 3 cut(s) 283, 306, 342
Hpy188I TCNGA 3 cut(s) 11, 49, 78
Hpy188III TCNNGA 3 cut(s) 121, 269, 326
Hpy8I GTNNAC 3 cut(s) 283, 306, 342
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 64, 127
HpyCH4V TGCA 2 cut(s) 311, 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 94
HpyF3I CTNAG 1 cut(s) 149
KspAI GTTAAC 1 cut(s) 283
Kzo9I GATC 5 cut(s) 6, 25, 131, 265, 322
LpnPI CCDG 9 cut(s) 28, 44, 92, 106, 130, 157, 184, 212, 254
Lsp1109I GCAGC 1 cut(s) 362
LweI GCATC 2 cut(s) 319, 320
MaeI CTAG 1 cut(s) 20
MalI GATC 5 cut(s) 8, 27, 133, 267, 324
MboI GATC 5 cut(s) 6, 25, 131, 265, 322
MboII GAAGA 2 cut(s) 35, 79
MflI RGATCY 3 cut(s) 6, 25, 265
MluCI AATT 2 cut(s) 42, 54
MlyI GAGTC 1 cut(s) 347
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 5 cut(s) 103, 144, 148, 247, 250
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 299
MspI CCGG 2 cut(s) 171, 199
MspR9I CCNGG 3 cut(s) 16, 145, 172
MvaI CCWGG 2 cut(s) 16, 145
MwoI GCNNNNNNNGC 1 cut(s) 94
NciI CCSGG 1 cut(s) 172
NdeII GATC 5 cut(s) 6, 25, 131, 265, 322
NlaIV GGNNCC 1 cut(s) 8
PdmI GAANNNNTTC 1 cut(s) 87
PfeI GAWTC 1 cut(s) 113
PkrI GCNGC 1 cut(s) 352
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
Psp6I CCWGG 2 cut(s) 14, 143
PspGI CCWGG 2 cut(s) 14, 143
PspN4I GGNNCC 1 cut(s) 8
PsuI RGATCY 3 cut(s) 6, 25, 265
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
RseI CAYNNNNRTG 1 cut(s) 299
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 1 cut(s) 351
Sau3AI GATC 5 cut(s) 6, 25, 131, 265, 322
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 3 cut(s) 16, 145, 172
SetI ASST 5 cut(s) 63, 146, 192, 196, 364
SfaNI GCATC 2 cut(s) 319, 320
SmiMI CAYNNNNRTG 1 cut(s) 299
Sse9I AATT 2 cut(s) 42, 54
SspI AATATT 1 cut(s) 319
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 3 cut(s) 14, 143, 170
TaqI TCGA 1 cut(s) 327
TasI AATT 2 cut(s) 42, 54
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 339
TseI GCWGC 1 cut(s) 350
TspDTI ATGAA 1 cut(s) 249
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 1 cut(s) 339
XagI CCTNNNNNAGG 1 cut(s) 148
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.