Rmu_sc0004974.1_g000007

Pleckstrin homology domain-containing family A member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004974.1
OG0012657
Physical Location & Seq
Forward (+)
34065 .. 38303
4239 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
atggcgggatctgttttcacttctgctttggaaggaatgaagcatgttaaatctgaaaatggagagatgctcaccaagcatttcttggatgtatgcaaacagttactacccgttttagataagtttggagcagcactttcacccgtcaaatctgatgttgctaacaatatatcgaggctggaaaccaagtactcttccaacccatcagaattcaatctattgtacagtctagttcggcctgagattgaagccaaaaccgctaaatcctcatccagttgcacaaatgcgctactatggctaactaggcatgttggaattaagcttgctcctgagaggaagaaattcatggagataataggcgaaacggctgatttgacttctgacattcagaagttctgcacaaagttctctccacttctcgaagagaatcataagttccttatgaatgagtttcaatctccaccaggatttgcatcagcacctttaagtcagaatggagagatgaccgacaataatatggtcaaatgcatctctgaagattcaagccggagaagttcaaggagttcaatgtctccgacgcatgaccctgaattgcccaagtcatcgagaaggcaatcatcacgggggcatgaaggaggtggcggtggaactgaatccccaagggaaaaatcagataaacctaggcaaacaagaaggccttctaggagggacttatcggagggtaatagaggcagccgtcaggaagccacagatacaagtagcgaatcctccctaccggatatccccaagaaatcacggcggaagaaatcaaaagaatcggcgattggggggtccaaaaaaaaaaaagatcctggcaccccatctacagcaaagtccaatagcattgagctatctcagaattcttttcaagagggggaagacaaagggtttacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GLTP

Protein Analysis

311

Amino Acids

34.2

Weight (kDa)

9.38

Isoelectric Point (pI)

73.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GLTP PF08718 24 - 103 5.5e-21 Glycolipid transfer protein (GLTP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 854
AciI CCGC 4 cut(s) 5, 258, 642, 799
AclWI GGATC 2 cut(s) 16, 842
AcsI RAATTY 3 cut(s) 209, 341, 898
AcuI CTGAAG 1 cut(s) 555
AfaI GTAC 2 cut(s) 191, 224
AgsI TTSAA 7 cut(s) 214, 248, 455, 543, 558, 567, 908
AjnI CCWGG 2 cut(s) 463, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 807, 839
AluBI AGCT 2 cut(s) 322, 889
AluI AGCT 2 cut(s) 322, 889
Alw26I GTCTC 1 cut(s) 576
AlwI GGATC 2 cut(s) 16, 842
AoxI GGCC 2 cut(s) 236, 695
ApeKI GCWGC 2 cut(s) 131, 732
ApoI RAATTY 3 cut(s) 209, 341, 898
Asp700I GAANNNNTTC 2 cut(s) 341, 697
AspA2I CCTAGG 1 cut(s) 680
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 1 cut(s) 831
AsuHPI GGTGA 2 cut(s) 64, 132
AvaII GGWCC 1 cut(s) 831
AvrII CCTAGG 1 cut(s) 680
BanI GGYRCC 1 cut(s) 854
BbsI GAAGAC 1 cut(s) 924
BbvI GCAGC 2 cut(s) 143, 744
BccI CCATC 2 cut(s) 211, 868
BceAI ACGGC 3 cut(s) 381, 720, 812
BciT130I CCWGG 2 cut(s) 465, 852
BcoDI GTCTC 1 cut(s) 576
BfaI CTAG 4 cut(s) 230, 303, 681, 702
BfmI CTRYAG 1 cut(s) 864
BisI GCNGC 2 cut(s) 132, 733
BlnI CCTAGG 1 cut(s) 680
BlsI GCNGC 2 cut(s) 133, 734
BmcAI AGTACT 1 cut(s) 191
Bme1390I CCNGG 2 cut(s) 465, 852
Bme18I GGWCC 1 cut(s) 831
BmgT120I GGNCC 1 cut(s) 831
BmiI GGNNCC 2 cut(s) 832, 856
BmrFI CCNGG 2 cut(s) 465, 852
BmsI GCATC 3 cut(s) 57, 482, 537
BpiI GAAGAC 1 cut(s) 924
BplI GAGNNNNNCTC 2 cut(s) 54, 86
BsaBI GATNNNNATC 1 cut(s) 472
BsaJI CCNNGG 2 cut(s) 659, 680
BsaWI WCCGGW 1 cut(s) 775
Bse1I ACTGG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 472
BseBI CCWGG 2 cut(s) 465, 852
BseDI CCNNGG 2 cut(s) 659, 680
BseGI GGATG 2 cut(s) 94, 269
BseJI GATNNNNATC 1 cut(s) 472
BseMII CTCAG 3 cut(s) 231, 321, 908
BseNI ACTGG 1 cut(s) 273
BseXI GCAGC 2 cut(s) 143, 744
BsgI GTGCAG 1 cut(s) 382
BshFI GGCC 2 cut(s) 238, 697
BshNI GGYRCC 1 cut(s) 854
BsiSI CCGG 2 cut(s) 547, 776
BslFI GGGAC 1 cut(s) 722
BsmAI GTCTC 1 cut(s) 576
BsmFI GGGAC 1 cut(s) 722
BsnI GGCC 2 cut(s) 238, 697
Bsp1407I TGTACA 1 cut(s) 222
Bsp143I GATC 2 cut(s) 8, 847
BspACI CCGC 4 cut(s) 5, 258, 642, 799
BspANI GGCC 2 cut(s) 238, 697
BspCNI CTCAG 3 cut(s) 232, 322, 907
BspLI GGNNCC 2 cut(s) 832, 856
BspPI GGATC 2 cut(s) 16, 842
BspT107I GGYRCC 1 cut(s) 854
BsrGI TGTACA 1 cut(s) 222
BsrI ACTGG 1 cut(s) 273
BssECI CCNNGG 2 cut(s) 659, 680
BssMI GATC 2 cut(s) 8, 847
BssT1I CCWWGG 2 cut(s) 659, 680
Bst2UI CCWGG 2 cut(s) 465, 852
Bst4CI ACNGT 2 cut(s) 102, 227
Bst6I CTCTTC 2 cut(s) 199, 417
BstAUI TGTACA 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 3 cut(s) 240, 330, 894
BstF5I GGATG 2 cut(s) 94, 269
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 2 cut(s) 11, 850
BstMAI GTCTC 1 cut(s) 576
BstMBI GATC 2 cut(s) 8, 847
BstMWI GCNNNNNNNGC 4 cut(s) 76, 257, 295, 304
BstNI CCWGG 2 cut(s) 465, 852
BstNSI RCATGY 2 cut(s) 47, 311
BstSCI CCNGG 2 cut(s) 463, 850
BstSFI CTRYAG 1 cut(s) 864
BstV1I GCAGC 2 cut(s) 143, 744
BstV2I GAAGAC 1 cut(s) 924
BstX2I RGATCY 2 cut(s) 8, 847
BstYI RGATCY 2 cut(s) 8, 847
BsuRI GGCC 2 cut(s) 238, 697
BtsCI GGATG 2 cut(s) 94, 269
Cac8I GCNNGC 1 cut(s) 324
CfoI GCGC 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 831
CseI GACGC 1 cut(s) 586
Csp6I GTAC 2 cut(s) 190, 223
CviAII CATG 6 cut(s) 44, 308, 346, 581, 629, 933
CviQI GTAC 2 cut(s) 190, 223
DdeI CTNAG 3 cut(s) 240, 330, 894
DpnI GATC 2 cut(s) 10, 849
DpnII GATC 2 cut(s) 8, 847
Eam1104I CTCTTC 2 cut(s) 199, 417
EarI CTCTTC 2 cut(s) 199, 417
EciI GGCGGA 1 cut(s) 814
Eco130I CCWWGG 2 cut(s) 659, 680
Eco147I AGGCCT 1 cut(s) 697
Eco32I GATATC 1 cut(s) 781
Eco47I GGWCC 1 cut(s) 831
Eco57I CTGAAG 1 cut(s) 555
EcoRI GAATTC 2 cut(s) 209, 898
EcoRII CCWGG 2 cut(s) 463, 850
EcoRV GATATC 1 cut(s) 781
EcoT14I CCWWGG 2 cut(s) 659, 680
EcoT22I ATGCAT 1 cut(s) 530
ErhI CCWWGG 2 cut(s) 659, 680
FaeI CATG 6 cut(s) 47, 311, 349, 584, 632, 936
FalI AAGNNNNNCTT 4 cut(s) 68, 100, 682, 714
FaqI GGGAC 1 cut(s) 722
FatI CATG 6 cut(s) 43, 307, 345, 580, 628, 932
Fnu4HI GCNGC 2 cut(s) 132, 733
FokI GGATG 2 cut(s) 101, 256
Fsp4HI GCNGC 2 cut(s) 132, 733
FspBI CTAG 4 cut(s) 230, 303, 681, 702
GlaI GCGC 1 cut(s) 288
GluI GCNGC 2 cut(s) 132, 733
HaeIII GGCC 2 cut(s) 238, 697
HapII CCGG 2 cut(s) 547, 776
HgaI GACGC 1 cut(s) 586
HhaI GCGC 1 cut(s) 289
Hin1II CATG 6 cut(s) 47, 311, 349, 584, 632, 936
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 5 cut(s) 427, 539, 653, 764, 815
HpaII CCGG 2 cut(s) 547, 776
HphI GGTGA 2 cut(s) 64, 132
Hpy166II GTNNAC 1 cut(s) 930
Hpy188III TCNNGA 5 cut(s) 329, 419, 606, 740, 908
Hpy8I GTNNAC 1 cut(s) 930
Hpy99I CGWCG 1 cut(s) 580
HpyAV CCTTC 5 cut(s) 26, 603, 626, 687, 708
HpyCH4III ACNGT 2 cut(s) 102, 227
HpyCH4V TGCA 5 cut(s) 96, 279, 399, 473, 528
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 257, 295, 304
HpyF3I CTNAG 3 cut(s) 240, 330, 894
Hsp92II CATG 6 cut(s) 47, 311, 349, 584, 632, 936
HspAI GCGC 1 cut(s) 287
Kzo9I GATC 2 cut(s) 8, 847
LmnI GCTCC 2 cut(s) 128, 331
Lsp1109I GCAGC 2 cut(s) 143, 744
LweI GCATC 3 cut(s) 57, 482, 537
MaeI CTAG 4 cut(s) 230, 303, 681, 702
MaeIII GTNAC 1 cut(s) 102
MalI GATC 2 cut(s) 10, 849
MboI GATC 2 cut(s) 8, 847
MboII GAAGA 6 cut(s) 186, 349, 434, 548, 814, 929
MflI RGATCY 2 cut(s) 8, 847
MluCI AATT 5 cut(s) 209, 315, 341, 590, 898
MmeI TCCRAC 3 cut(s) 222, 292, 599
MnlI CCTC 9 cut(s) 168, 277, 327, 629, 699, 712, 722, 778, 904
Mph1103I ATGCAT 1 cut(s) 530
MroXI GAANNNNTTC 2 cut(s) 341, 697
MseI TTAA 3 cut(s) 48, 318, 485
MspI CCGG 2 cut(s) 547, 776
MspR9I CCNGG 2 cut(s) 465, 852
MvaI CCWGG 2 cut(s) 465, 852
MwoI GCNNNNNNNGC 4 cut(s) 76, 257, 295, 304
NdeII GATC 2 cut(s) 8, 847
NlaIII CATG 6 cut(s) 47, 311, 349, 584, 632, 936
NlaIV GGNNCC 2 cut(s) 832, 856
NsiI ATGCAT 1 cut(s) 530
NspI RCATGY 2 cut(s) 47, 311
PceI AGGCCT 1 cut(s) 697
PdmI GAANNNNTTC 2 cut(s) 341, 697
PfeI GAWTC 5 cut(s) 427, 539, 653, 764, 815
PkrI GCNGC 2 cut(s) 133, 734
Psp6I CCWGG 2 cut(s) 463, 850
PspGI CCWGG 2 cut(s) 463, 850
PspN4I GGNNCC 2 cut(s) 832, 856
PspPI GGNCC 1 cut(s) 831
PsuI RGATCY 2 cut(s) 8, 847
RsaI GTAC 2 cut(s) 191, 224
RsaNI GTAC 2 cut(s) 190, 223
SaqAI TTAA 3 cut(s) 48, 318, 485
SatI GCNGC 2 cut(s) 132, 733
Sau3AI GATC 2 cut(s) 8, 847
Sau96I GGNCC 1 cut(s) 831
ScaI AGTACT 1 cut(s) 191
ScrFI CCNGG 2 cut(s) 465, 852
SetI ASST 5 cut(s) 324, 484, 640, 682, 891
SfaNI GCATC 3 cut(s) 57, 482, 537
SfcI CTRYAG 1 cut(s) 864
SinI GGWCC 1 cut(s) 831
Sse9I AATT 5 cut(s) 209, 315, 341, 590, 898
SseBI AGGCCT 1 cut(s) 697
SsiI CCGC 4 cut(s) 5, 258, 642, 799
SspMI CTAG 4 cut(s) 230, 303, 681, 702
StuI AGGCCT 1 cut(s) 697
StyD4I CCNGG 2 cut(s) 463, 850
StyI CCWWGG 2 cut(s) 659, 680
TaaI ACNGT 2 cut(s) 102, 227
TaqI TCGA 3 cut(s) 173, 420, 605
TaqII GACCGA 1 cut(s) 521
TasI AATT 5 cut(s) 209, 315, 341, 590, 898
TatI WGTACW 2 cut(s) 189, 222
TfiI GAWTC 5 cut(s) 427, 539, 653, 764, 815
Tru1I TTAA 3 cut(s) 48, 318, 485
Tru9I TTAA 3 cut(s) 48, 318, 485
TseI GCWGC 2 cut(s) 131, 732
TspDTI ATGAA 4 cut(s) 53, 334, 458, 645
VpaK11BI GGWCC 1 cut(s) 831
XapI RAATTY 3 cut(s) 209, 341, 898
XceI RCATGY 2 cut(s) 47, 311
XcmI CCANNNNNNNNNTGG 1 cut(s) 82
XmaJI CCTAGG 1 cut(s) 680
XmnI GAANNNNTTC 2 cut(s) 341, 697
XspI CTAG 4 cut(s) 230, 303, 681, 702
ZrmI AGTACT 1 cut(s) 191
Zsp2I ATGCAT 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.