Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0005550.1

Orthogroup ID

OG0011250

Physical Location & Seq

Genomic Coordinates Reverse (-)

21447 .. 26888

Gene Structure 5442 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1908 bp

atgtcgtcggagatcgaggtcgtcgaggacgacgagttccaaacccgcggcgatcacgaatcggcggcggcggcggcggcggtagccggttcggtcaattcgggcggtggagaagccaatgggattggggaggagagtctgcgaaacgacgtgtacacggcggcggcctacggcgatttggagaagcttcagagattggtggagtgtgagggttgctcggtttccgagaccgatgggctgggctactacgcgctccagtgggccgcccttaacaaccgaactgccgccgcccagtatattatagagcatggaggagatgtgaatgcgaaggaccatgcggggcagacggcattgcactggagtgcagtgcggggtgctatccaggttgcggagctattactccaagagggtgcgagggtgaatgcgggtgatatgtatggctatcagactacacatgttgcggcccaatatggtcagactgctttcctataccatattgtttcaaaatggaatgctgaccctgatgttcctgataatgatggaagaagccctttgcactgggctgcttataaaggttttgctgattgtatacgtcttcttttgtttctggatgcatatagagggcgccaagataaagagggttgcactcctctacattgggctgctatcaggggtaacttggaggcatgcacagttttggtacaagctgggaaaaaggaggacttgatggttacagataacactggccttacaccagcacaacttgcttctgataaaaaccacagacaagttgcttttttccttgggaatgctcgaaggctgcttgacaaacgttgtgatgggaacagtcgtcttggacaactttcaaaactaggacttgcaccaggactttggtgtataatctttgtgctccttgtgacctatactcattctgttatcatggcaccaaatctgccaaagttaacagccggctcagtttttattgcatggcttggagtttttctagcaactgcagggctggttatgttctatcggtgtagcagtaaagatcccggttacatccgaatgaatatccatgattcacaaaatatgaaagatgatgaacctcttttgaagattgagataaataatccggccttgctagctggtaactggtctcagctctgtgcaacttgcaagattgtcagacctcttcgcgcaaagcactgttcaacttgtgaccgttgtgttgaacaatttgaccaccattgcccttgggtatccaattgcattggcaagaaaaacaaatgggatttctttattttccttgttcttgaagttttggcaatgttgactactggaggagtcactcttacgagaatttggagtgatccactcagtccatcttcatttggagcatggcttaatcatgttggtacaagtcatatcggtgctatatcatttttgattgttgatttcttcctcttcttcggtgtggtcgttttgactgtcgtacaagcttctcaaatcgcgcgaaatattacaactaatgaaatggcaaatgcaatgcgctataactaccttagagggccaggtggtcgcttcagaaacccatatgatcatgggatgaagaagaattgctcagatttcttaatcaagggctacaatgaagatgtggaattgattgaagaatcaactcgtgctgaggggactggtatgaggcacatgaatggggattcccattctcaccactcaaatggcaatggtcatgttgcaatcaacgtaaattcgaatgcatcagcacatcaaggtcatgttcattctgcccattgcagccataacaatcatggtaaaccaaaaactgataatgtccccgtaggcttgggtcttggccttggacggaacgcttcccgacctactgtatcttag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000035 GO:0000902 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0012505 GO:0016043 GO:0016049 GO:0031410 GO:0031982 GO:0031984 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0060560 GO:0071840 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_3 Ank_4 DHHC

Protein Analysis

635

Amino Acids

69.34

Weight (kDa)

6.39

Isoelectric Point (pI)

30.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	53 - 143	2.3e-15	Ankyrin repeats (3 copies)
Ank_4	PF13637	87 - 134	7e-07	Ankyrin repeats (many copies)
Ank_2	PF12796	152 - 238	3.4e-16	Ankyrin repeats (3 copies)
Ank_2	PF12796	208 - 268	4.1e-08	Ankyrin repeats (3 copies)
DHHC	PF01529	387 - 514	2.8e-32	DHHC palmitoyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20350
fragaria_vesca	FvH4_1g02030 FvH4_1g02030 FvH4_1g02030
malus_domestica	MD02G1018500.v1.1 MD15G1162500.v1.1
prunus_persica	Prupe.7G252300_v2.0.a1
pyrus_communis	pycom02g01660 pycom15g14610
rosa_chinensis	RchiOBHm_Chr2g0087091
rosa_laevigata	RLG00000015831
rosa_multiflora	Rmu_sc0005550.1_g000004
rosa_roxburghii	Rroxscaffold_2G00153890 Rroxscaffold_3G00239100
rosa_rugosa	Rorug01G0472400
rosa_samantha	Rh2AG025700 Rh2BG025400 Rh2CG026000 Rh2DG025900
rosa_wichuraiana	Rw2G002040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	570
AccB1I	GGYRCC	2 cut(s)	624, 943
AccI	GTMKAC	1 cut(s)	589
AccII	CGCG	5 cut(s)	48, 251, 1197, 1511, 1513
AclI	AACGTT	1 cut(s)	832
AclWI	GGATC	2 cut(s)	1043, 1364
AcsI	RAATTY	2 cut(s)	1359, 1765
AcuI	CTGAAG	2 cut(s)	173, 1567
AcyI	GRCGYC	1 cut(s)	625
AfaI	GTAC	4 cut(s)	155, 702, 1417, 1494
AfiI	CCNNNNNNNGG	1 cut(s)	388
AflIII	ACRYGT	2 cut(s)	150, 454
AgsI	TTSAA	7 cut(s)	504, 867, 1114, 1212, 1232, 1316, 1667
AjiI	CACGTC	1 cut(s)	151
AjnI	CCWGG	3 cut(s)	381, 883, 1570
AleI	CACNNNNGTG	1 cut(s)	360
AloI	GAACNNNNNNTCC	2 cut(s)	20, 52
AluBI	AGCT	6 cut(s)	187, 394, 707, 1145, 1162, 1499
AluI	AGCT	6 cut(s)	187, 394, 707, 1145, 1162, 1499
Alw21I	GWGCWC	1 cut(s)	912
Alw26I	GTCTC	2 cut(s)	221, 1161
AlwI	GGATC	2 cut(s)	1043, 1364
AoxI	GGCC	7 cut(s)	165, 261, 462, 745, 1134, 1568, 1870
ApeKI	GCWGC	4 cut(s)	563, 662, 820, 1812
ApoI	RAATTY	2 cut(s)	1359, 1765
AspLEI	GCGC	5 cut(s)	253, 627, 1199, 1513, 1551
AspS9I	GGNCC	4 cut(s)	261, 331, 463, 1568
AsuC2I	CCSGG	1 cut(s)	1053
AsuHPI	GGTGA	3 cut(s)	430, 440, 1718
AsuII	TTCGAA	1 cut(s)	1769
AsuNHI	GCTAGC	1 cut(s)	1141
AvaII	GGWCC	1 cut(s)	331
BanI	GGYRCC	2 cut(s)	624, 943
BauI	CACGAG	1 cut(s)	1677
BbsI	GAAGAC	1 cut(s)	587
Bbv12I	GWGCWC	1 cut(s)	912
BbvCI	CCTCAGC	1 cut(s)	1683
BbvI	GCAGC	4 cut(s)	550, 649, 807, 1824
BccI	CCATC	5 cut(s)	227, 533, 721, 833, 1390
BceAI	ACGGC	3 cut(s)	174, 187, 363
BciT130I	CCWGG	3 cut(s)	383, 885, 1572
BciVI	GTATCC	1 cut(s)	1270
BclI	TGATCA	1 cut(s)	1597
BcnI	CCSGG	1 cut(s)	1053
BcoDI	GTCTC	2 cut(s)	221, 1161
BfaI	CTAG	3 cut(s)	872, 1004, 1142
BfmI	CTRYAG	1 cut(s)	1011
BfoI	RGCGCY	1 cut(s)	628
BfuI	GTATCC	1 cut(s)	1270
Bme1390I	CCNGG	4 cut(s)	383, 885, 1053, 1572
Bme18I	GGWCC	1 cut(s)	331
BmgBI	CACGTC	1 cut(s)	151
BmgT120I	GGNCC	4 cut(s)	261, 331, 463, 1568
BmiI	GGNNCC	2 cut(s)	626, 945
BmrFI	CCNGG	4 cut(s)	383, 885, 1053, 1572
BmrI	ACTGGG	2 cut(s)	286, 568
BmsI	GCATC	2 cut(s)	601, 1784
BmtI	GCTAGC	1 cut(s)	1145
BmuI	ACTGGG	2 cut(s)	286, 568
BpiI	GAAGAC	1 cut(s)	587
BplI	GAGNNNNNCTC	2 cut(s)	200, 232
BpmI	CTGGAG	3 cut(s)	239, 379, 1359
Bpu10I	CCTNAGC	1 cut(s)	1683
Bpu14I	TTCGAA	1 cut(s)	1769
BpuMI	CCSGG	1 cut(s)	1053
BsaHI	GRCGYC	1 cut(s)	625
BsaI	GGTCTC	2 cut(s)	221, 1161
BsaJI	CCNNGG	4 cut(s)	46, 802, 1253, 1873
BsaXI	ACNNNNNCTCC	2 cut(s)	303, 333
Bsc4I	CCNNNNNNNGG	1 cut(s)	388
Bse118I	RCCGGY	2 cut(s)	86, 968
Bse1I	ACTGG	8 cut(s)	256, 292, 362, 563, 748, 1157, 1342, 1696
Bse3DI	GCAATG	6 cut(s)	350, 1246, 1332, 1551, 1747, 1807
BseBI	CCWGG	3 cut(s)	383, 885, 1572
BseDI	CCNNGG	4 cut(s)	46, 802, 1253, 1873
BseGI	GGATG	3 cut(s)	616, 1059, 1611
BseLI	CCNNNNNNNGG	1 cut(s)	388
BseMI	GCAATG	6 cut(s)	350, 1246, 1332, 1551, 1747, 1807
BseMII	CTCAG	5 cut(s)	987, 1172, 1390, 1635, 1674
BseNI	ACTGG	8 cut(s)	256, 292, 362, 563, 748, 1157, 1342, 1696
BseRI	GAGGAG	4 cut(s)	146, 327, 639, 1356
BseXI	GCAGC	4 cut(s)	550, 649, 807, 1824
BseYI	CCCAGC	2 cut(s)	238, 707
BsgI	GTGCAG	1 cut(s)	384
Bsh1236I	CGCG	5 cut(s)	48, 251, 1197, 1511, 1513
BshFI	GGCC	7 cut(s)	167, 263, 464, 747, 1136, 1570, 1872
BshNI	GGYRCC	2 cut(s)	624, 943
BsiHKAI	GWGCWC	1 cut(s)	912
BsiSI	CCGG	4 cut(s)	87, 969, 1053, 1133
BslFI	GGGAC	2 cut(s)	1702, 1835
BslI	CCNNNNNNNGG	1 cut(s)	388
BsmAI	GTCTC	2 cut(s)	221, 1161
BsmFI	GGGAC	2 cut(s)	1702, 1835
BsmI	GAATGC	5 cut(s)	328, 427, 517, 814, 1777
BsnI	GGCC	7 cut(s)	167, 263, 464, 747, 1136, 1570, 1872
Bso31I	GGTCTC	2 cut(s)	221, 1161
Bsp119I	TTCGAA	1 cut(s)	1769
Bsp1286I	GDGCHC	1 cut(s)	912
Bsp1407I	TGTACA	1 cut(s)	153
Bsp143I	GATC	5 cut(s)	12, 52, 1048, 1369, 1597
BspANI	GGCC	7 cut(s)	167, 263, 464, 747, 1136, 1570, 1872
BspCNI	CTCAG	5 cut(s)	986, 1171, 1389, 1634, 1675
BspFNI	CGCG	5 cut(s)	48, 251, 1197, 1511, 1513
BspLI	GGNNCC	2 cut(s)	626, 945
BspMAI	CTGCAG	1 cut(s)	1015
BspOI	GCTAGC	1 cut(s)	1145
BspPI	GGATC	2 cut(s)	1043, 1364
BspT104I	TTCGAA	1 cut(s)	1769
BspT107I	GGYRCC	2 cut(s)	624, 943
BspTNI	GGTCTC	2 cut(s)	221, 1161
BsrDI	GCAATG	6 cut(s)	350, 1246, 1332, 1551, 1747, 1807
BsrFI	RCCGGY	2 cut(s)	86, 968
BsrGI	TGTACA	1 cut(s)	153
BsrI	ACTGG	8 cut(s)	256, 292, 362, 563, 748, 1157, 1342, 1696
BssAI	RCCGGY	2 cut(s)	86, 968
BssECI	CCNNGG	4 cut(s)	46, 802, 1253, 1873
BssMI	GATC	5 cut(s)	12, 52, 1048, 1369, 1597
BssNAI	GTATAC	1 cut(s)	590
BssNI	GRCGYC	1 cut(s)	625
BssSI	CACGAG	1 cut(s)	1677
BssT1I	CCWWGG	3 cut(s)	802, 1253, 1873
Bst1107I	GTATAC	1 cut(s)	590
Bst2BI	CACGAG	1 cut(s)	1677
Bst2UI	CCWGG	3 cut(s)	383, 885, 1572
Bst4CI	ACNGT	6 cut(s)	694, 848, 1208, 1223, 1489, 1900
Bst6I	CTCTTC	2 cut(s)	1197, 1469
BstACI	GRCGYC	1 cut(s)	625
BstAPI	GCANNNNNTGC	1 cut(s)	764
BstAUI	TGTACA	1 cut(s)	153
BstBI	TTCGAA	1 cut(s)	1769
BstC8I	GCNNGC	3 cut(s)	688, 970, 1143
BstDEI	CTNAG	7 cut(s)	973, 1158, 1376, 1562, 1621, 1683, 1905
BstDSI	CCRYGG	1 cut(s)	46
BstF5I	GGATG	3 cut(s)	616, 1059, 1611
BstFNI	CGCG	5 cut(s)	48, 251, 1197, 1511, 1513
BstH2I	RGCGCY	1 cut(s)	628
BstHHI	GCGC	5 cut(s)	253, 627, 1199, 1513, 1551
BstKTI	GATC	5 cut(s)	15, 55, 1051, 1372, 1600
BstMAI	GTCTC	2 cut(s)	221, 1161
BstMBI	GATC	5 cut(s)	12, 52, 1048, 1369, 1597
BstMWI	GCNNNNNNNGC	6 cut(s)	71, 74, 77, 83, 764, 1142
BstNI	CCWGG	3 cut(s)	383, 885, 1572
BstNSI	RCATGY	2 cut(s)	458, 690
BstSCI	CCNGG	4 cut(s)	381, 883, 1051, 1570
BstSFI	CTRYAG	1 cut(s)	1011
BstUI	CGCG	5 cut(s)	48, 251, 1197, 1511, 1513
BstV1I	GCAGC	4 cut(s)	550, 649, 807, 1824
BstV2I	GAAGAC	1 cut(s)	587
BstX2I	RGATCY	1 cut(s)	1048
BstXI	CCANNNNNNTGG	2 cut(s)	891, 1736
BstYI	RGATCY	1 cut(s)	1048
BstZ17I	GTATAC	1 cut(s)	590
BsuI	GTATCC	1 cut(s)	1270
BsuRI	GGCC	7 cut(s)	167, 263, 464, 747, 1136, 1570, 1872
BtgI	CCRYGG	1 cut(s)	46
BtrI	CACGTC	1 cut(s)	151
BtsCI	GGATG	3 cut(s)	616, 1059, 1611
BtsI	GCAGTG	1 cut(s)	372
BtsIMutI	CAGTG	6 cut(s)	263, 355, 372, 556, 741, 1204
Cac8I	GCNNGC	3 cut(s)	688, 970, 1143
CfoI	GCGC	5 cut(s)	253, 627, 1199, 1513, 1551
Cfr10I	RCCGGY	2 cut(s)	86, 968
Cfr13I	GGNCC	4 cut(s)	261, 331, 463, 1568
Cfr42I	CCGCGG	1 cut(s)	49
Csp6I	GTAC	4 cut(s)	154, 701, 1416, 1493
CviQI	GTAC	4 cut(s)	154, 701, 1416, 1493
DdeI	CTNAG	7 cut(s)	973, 1158, 1376, 1562, 1621, 1683, 1905
DinI	GGCGCC	1 cut(s)	626
DpnI	GATC	5 cut(s)	14, 54, 1050, 1371, 1599
DpnII	GATC	5 cut(s)	12, 52, 1048, 1369, 1597
Eam1104I	CTCTTC	2 cut(s)	1197, 1469
EarI	CTCTTC	2 cut(s)	1197, 1469
Eco130I	CCWWGG	3 cut(s)	802, 1253, 1873
Eco31I	GGTCTC	2 cut(s)	221, 1161
Eco47I	GGWCC	1 cut(s)	331
Eco57I	CTGAAG	2 cut(s)	173, 1567
EcoRII	CCWGG	3 cut(s)	381, 883, 1570
EcoT14I	CCWWGG	3 cut(s)	802, 1253, 1873
EcoT22I	ATGCAT	2 cut(s)	616, 1777
EgeI	GGCGCC	1 cut(s)	626
EheI	GGCGCC	1 cut(s)	626
ErhI	CCWWGG	3 cut(s)	802, 1253, 1873
FalI	AAGNNNNNCTT	4 cut(s)	535, 567, 707, 739
FaqI	GGGAC	2 cut(s)	1702, 1835
FauI	CCCGC	4 cut(s)	53, 331, 363, 418
FauNDI	CATATG	1 cut(s)	1594
FbaI	TGATCA	1 cut(s)	1597
FblI	GTMKAC	1 cut(s)	589
FokI	GGATG	3 cut(s)	623, 1046, 1618
FspBI	CTAG	3 cut(s)	872, 1004, 1142
GlaI	GCGC	5 cut(s)	252, 626, 1198, 1512, 1550
GsaI	CCCAGC	2 cut(s)	242, 711
GsuI	CTGGAG	3 cut(s)	239, 379, 1359
HaeII	RGCGCY	1 cut(s)	628
HaeIII	GGCC	7 cut(s)	167, 263, 464, 747, 1136, 1570, 1872
HapII	CCGG	4 cut(s)	87, 969, 1053, 1133
HhaI	GCGC	5 cut(s)	253, 627, 1199, 1513, 1551
Hin1I	GRCGYC	1 cut(s)	625
Hin6I	GCGC	5 cut(s)	251, 625, 1197, 1511, 1549
HinP1I	GCGC	5 cut(s)	251, 625, 1197, 1511, 1549
HincII	GTYRAC	2 cut(s)	963, 1332
HindII	GTYRAC	2 cut(s)	963, 1332
HindIII	AAGCTT	2 cut(s)	185, 1497
HinfI	GANTC	6 cut(s)	59, 136, 1079, 1344, 1670, 1715
HpaI	GTTAAC	1 cut(s)	963
HpaII	CCGG	4 cut(s)	87, 969, 1053, 1133
HphI	GGTGA	3 cut(s)	430, 440, 1718
Hpy166II	GTNNAC	6 cut(s)	154, 156, 590, 963, 1332, 1832
Hpy188III	TCNNGA	5 cut(s)	56, 530, 608, 1313, 1890
Hpy8I	GTNNAC	6 cut(s)	154, 156, 590, 963, 1332, 1832
Hpy99I	CGWCG	4 cut(s)	10, 26, 35, 152
HpyAV	CCTTC	2 cut(s)	322, 810
HpyCH4III	ACNGT	6 cut(s)	694, 848, 1208, 1223, 1489, 1900
HpyCH4IV	ACGT	4 cut(s)	150, 592, 832, 1761
HpyF10VI	GCNNNNNNNGC	6 cut(s)	71, 74, 77, 83, 764, 1142
HpyF3I	CTNAG	7 cut(s)	973, 1158, 1376, 1562, 1621, 1683, 1905
HpySE526I	ACGT	4 cut(s)	150, 592, 832, 1761
Hsp92I	GRCGYC	1 cut(s)	625
HspAI	GCGC	5 cut(s)	251, 625, 1197, 1511, 1549
KasI	GGCGCC	1 cut(s)	624
KroI	GCCGGC	1 cut(s)	968
KroNI	GCCGGC	1 cut(s)	970
Ksp22I	TGATCA	1 cut(s)	1597
KspAI	GTTAAC	1 cut(s)	963
KspI	CCGCGG	1 cut(s)	49
Kzo9I	GATC	5 cut(s)	12, 52, 1048, 1369, 1597
LmnI	GCTCC	4 cut(s)	258, 391, 915, 1394
Lsp1109I	GCAGC	4 cut(s)	550, 649, 807, 1824
LweI	GCATC	2 cut(s)	601, 1784
MaeI	CTAG	3 cut(s)	872, 1004, 1142
MaeII	ACGT	4 cut(s)	150, 592, 832, 1761
MaeIII	GTNAC	7 cut(s)	674, 730, 916, 1055, 1148, 1217, 1345
MalI	GATC	5 cut(s)	14, 54, 1050, 1371, 1599
MboI	GATC	5 cut(s)	12, 52, 1048, 1369, 1597
MfeI	CAATTG	1 cut(s)	1264
MflI	RGATCY	1 cut(s)	1048
MhlI	GDGCHC	1 cut(s)	912
MluCI	AATT	7 cut(s)	97, 1235, 1264, 1359, 1615, 1658, 1765
Mly113I	GGCGCC	1 cut(s)	625
MlyI	GAGTC	2 cut(s)	145, 1353
Mph1103I	ATGCAT	2 cut(s)	616, 1777
MroNI	GCCGGC	1 cut(s)	968
MseI	TTAA	4 cut(s)	270, 962, 1404, 1631
MslI	CAYNNNNRTG	5 cut(s)	360, 1064, 1428, 1707, 1734
MspA1I	CMGCKG	1 cut(s)	48
MspI	CCGG	4 cut(s)	87, 969, 1053, 1133
MspR9I	CCNGG	4 cut(s)	383, 885, 1053, 1572
MunI	CAATTG	1 cut(s)	1264
Mva1269I	GAATGC	5 cut(s)	328, 427, 517, 814, 1777
MvaI	CCWGG	3 cut(s)	383, 885, 1572
MvnI	CGCG	5 cut(s)	48, 251, 1197, 1511, 1513
MwoI	GCNNNNNNNGC	6 cut(s)	71, 74, 77, 83, 764, 1142
NaeI	GCCGGC	1 cut(s)	970
NarI	GGCGCC	1 cut(s)	625
NciI	CCSGG	1 cut(s)	1053
NdeI	CATATG	1 cut(s)	1594
NdeII	GATC	5 cut(s)	12, 52, 1048, 1369, 1597
NgoMIV	GCCGGC	1 cut(s)	968
NheI	GCTAGC	1 cut(s)	1141
NlaIV	GGNNCC	2 cut(s)	626, 945
NmuCI	GTSAC	3 cut(s)	916, 1217, 1345
NsiI	ATGCAT	2 cut(s)	616, 1777
NspI	RCATGY	2 cut(s)	458, 690
NspV	TTCGAA	1 cut(s)	1769
OliI	CACNNNNGTG	1 cut(s)	360
PaeI	GCATGC	1 cut(s)	690
PciI	ACATGT	1 cut(s)	454
PcsI	WCGNNNNNNNCGW	4 cut(s)	21, 27, 30, 1476
PctI	GAATGC	5 cut(s)	328, 427, 517, 814, 1777
PdiI	GCCGGC	1 cut(s)	970
PfeI	GAWTC	4 cut(s)	59, 1079, 1670, 1715
PleI	GAGTC	2 cut(s)	144, 1352
PluTI	GGCGCC	1 cut(s)	628
PpsI	GAGTC	2 cut(s)	144, 1352
PscI	ACATGT	1 cut(s)	454
PsiI	TTATAA	1 cut(s)	570
Psp1406I	AACGTT	1 cut(s)	832
Psp6I	CCWGG	3 cut(s)	381, 883, 1570
PspFI	CCCAGC	2 cut(s)	238, 707
PspGI	CCWGG	3 cut(s)	381, 883, 1570
PspN4I	GGNNCC	2 cut(s)	626, 945
PspPI	GGNCC	4 cut(s)	261, 331, 463, 1568
PstI	CTGCAG	1 cut(s)	1015
PsuI	RGATCY	1 cut(s)	1048
RsaI	GTAC	4 cut(s)	155, 702, 1417, 1494
RsaNI	GTAC	4 cut(s)	154, 701, 1416, 1493
RseI	CAYNNNNRTG	5 cut(s)	360, 1064, 1428, 1707, 1734
SacII	CCGCGG	1 cut(s)	49
SaqAI	TTAA	4 cut(s)	270, 962, 1404, 1631
Sau3AI	GATC	5 cut(s)	12, 52, 1048, 1369, 1597
Sau96I	GGNCC	4 cut(s)	261, 331, 463, 1568
SchI	GAGTC	2 cut(s)	145, 1353
ScrFI	CCNGG	4 cut(s)	383, 885, 1053, 1572
SduI	GDGCHC	1 cut(s)	912
SfaNI	GCATC	2 cut(s)	601, 1784
SfcI	CTRYAG	1 cut(s)	1011
SfoI	GGCGCC	1 cut(s)	626
Sfr303I	CCGCGG	1 cut(s)	49
SfuI	TTCGAA	1 cut(s)	1769
SgrBI	CCGCGG	1 cut(s)	49
SinI	GGWCC	1 cut(s)	331
SmiMI	CAYNNNNRTG	5 cut(s)	360, 1064, 1428, 1707, 1734
SphI	GCATGC	1 cut(s)	690
Sse9I	AATT	7 cut(s)	97, 1235, 1264, 1359, 1615, 1658, 1765
SspDI	GGCGCC	1 cut(s)	624
SspI	AATATT	1 cut(s)	1519
SspMI	CTAG	3 cut(s)	872, 1004, 1142
StyD4I	CCNGG	4 cut(s)	381, 883, 1051, 1570
StyI	CCWWGG	3 cut(s)	802, 1253, 1873
TaaI	ACNGT	6 cut(s)	694, 848, 1208, 1223, 1489, 1900
TaiI	ACGT	4 cut(s)	153, 595, 835, 1764
TaqI	TCGA	4 cut(s)	15, 24, 814, 1769
TaqII	GACCGA	2 cut(s)	82, 245
TasI	AATT	7 cut(s)	97, 1235, 1264, 1359, 1615, 1658, 1765
TatI	WGTACW	1 cut(s)	153
TfiI	GAWTC	4 cut(s)	59, 1079, 1670, 1715
Tru1I	TTAA	4 cut(s)	270, 962, 1404, 1631
Tru9I	TTAA	4 cut(s)	270, 962, 1404, 1631
TscAI	CASTG	6 cut(s)	263, 362, 372, 563, 748, 1211
TseFI	GTSAC	3 cut(s)	916, 1217, 1345
TseI	GCWGC	4 cut(s)	563, 662, 820, 1812
Tsp45I	GTSAC	3 cut(s)	916, 1217, 1345
TspDTI	ATGAA	9 cut(s)	1082, 1106, 1116, 1377, 1545, 1622, 1662, 1721, 1787
TspGWI	ACGGA	1 cut(s)	1894
TspRI	CASTG	6 cut(s)	263, 362, 372, 563, 748, 1211
VpaK11BI	GGWCC	1 cut(s)	331
XapI	RAATTY	2 cut(s)	1359, 1765
XceI	RCATGY	2 cut(s)	458, 690
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1823
XmiI	GTMKAC	1 cut(s)	589
XspI	CTAG	3 cut(s)	872, 1004, 1142
Zsp2I	ATGCAT	2 cut(s)	616, 1777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.