Rmu_sc0005570.1_g000016

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005570.1
OG0014818
Physical Location & Seq
Reverse (-)
82664 .. 83487
824 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
atggagtcgataatcgtcccccatgaagctgatcataaattgccgggagcagttggaagtggtacggaaaatagatcagtggaaaccacttccttaaggcaaagatttcttgcttcaattggtgcccatgaatacttttcaccagagatgtggagagcagctcttacagagtttgtagcaactgcgtgtctaatgttcaccttgacgtcttcaatcatctcatgcttggattcacatgaggttgatcccaagctattagtaccctttgcggtcttcatcatagccttccttttcctcttggtgacagttcctttatctggtggtcatatgagtcctgttttcacattcatagctgccctaaacggtgttataactcttgctagagcttccatctacatcttgatgcaatgtgttggttcaattgtgggatttcttattatcaagactgtgatggaccataatacatcccaaaagtactccttaggtggctgcacaacacatggagtaggcacccaaacagcattgatgttagaatttgtttgcacatttgtggttctctttgttggagtcactgtagcattcgacaagaaaaggtgcaaggaacttggtttagcaatggtgtgtctcgtggtggccggggctatggcacttgcagtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko04962 ko04976 map04962 map04976
Pfam Domains
Protein Families
MIP

Protein Analysis

219

Amino Acids

23.51

Weight (kDa)

6.36

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 47 - 214 1.1e-20 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AatII GACGTC 1 cut(s) 209
AccB1I GGYRCC 2 cut(s) 122, 509
AciI CCGC 1 cut(s) 269
AclWI GGATC 1 cut(s) 239
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 1 cut(s) 533
AcyI GRCGYC 1 cut(s) 206
AfaI GTAC 3 cut(s) 64, 261, 476
AfiI CCNNNNNNNGG 1 cut(s) 317
AflII CTTAAG 1 cut(s) 94
AgsI TTSAA 3 cut(s) 117, 213, 420
AleI CACNNNNGTG 1 cut(s) 548
AluBI AGCT 5 cut(s) 29, 161, 253, 353, 386
AluI AGCT 5 cut(s) 29, 161, 253, 353, 386
Alw26I GTCTC 1 cut(s) 629
AlwI GGATC 1 cut(s) 239
AoxI GGCC 1 cut(s) 633
ApeKI GCWGC 3 cut(s) 158, 353, 489
ApoI RAATTY 1 cut(s) 533
AspS9I GGNCC 1 cut(s) 454
AsuC2I CCSGG 2 cut(s) 45, 637
AsuHPI GGTGA 3 cut(s) 132, 190, 313
AvaII GGWCC 1 cut(s) 454
AxyI CCTNAGG 1 cut(s) 481
BaeGI GKGCMC 1 cut(s) 127
BanI GGYRCC 2 cut(s) 122, 509
BauI CACGAG 1 cut(s) 626
BbsI GAAGAC 2 cut(s) 201, 265
BbvI GCAGC 3 cut(s) 170, 340, 476
BccI CCATC 2 cut(s) 398, 445
BclI TGATCA 1 cut(s) 31
BcnI CCSGG 2 cut(s) 45, 637
BcoDI GTCTC 1 cut(s) 629
BfaI CTAG 1 cut(s) 381
BfmI CTRYAG 1 cut(s) 573
BfrI CTTAAG 1 cut(s) 94
BisI GCNGC 3 cut(s) 159, 354, 490
BlsI GCNGC 3 cut(s) 160, 355, 491
BmcAI AGTACT 1 cut(s) 476
Bme1390I CCNGG 2 cut(s) 45, 637
Bme18I GGWCC 1 cut(s) 454
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 2 cut(s) 124, 511
BmrFI CCNGG 2 cut(s) 45, 637
BmsI GCATC 1 cut(s) 393
BpiI GAAGAC 2 cut(s) 201, 265
BplI GAGNNNNNCTC 2 cut(s) 145, 177
BpuMI CCSGG 2 cut(s) 45, 637
BsaHI GRCGYC 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 636
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse21I CCTNAGG 1 cut(s) 481
Bse3DI GCAATG 2 cut(s) 413, 621
BseDI CCNNGG 1 cut(s) 636
BseGI GGATG 1 cut(s) 464
BseLI CCNNNNNNNGG 1 cut(s) 317
BseMI GCAATG 2 cut(s) 413, 621
BseSI GKGCMC 1 cut(s) 127
BseXI GCAGC 3 cut(s) 170, 340, 476
BsgI GTGCAG 1 cut(s) 475
BshFI GGCC 1 cut(s) 635
BshNI GGYRCC 2 cut(s) 122, 509
BsiSI CCGG 2 cut(s) 44, 636
BslFI GGGAC 1 cut(s) 2
BslI CCNNNNNNNGG 1 cut(s) 317
BsmAI GTCTC 1 cut(s) 629
BsmFI GGGAC 1 cut(s) 2
BsmI GAATGC 1 cut(s) 578
BsnI GGCC 1 cut(s) 635
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 3 cut(s) 31, 74, 244
BspACI CCGC 1 cut(s) 269
BspANI GGCC 1 cut(s) 635
BspLI GGNNCC 2 cut(s) 124, 511
BspPI GGATC 1 cut(s) 239
BspT107I GGYRCC 2 cut(s) 122, 509
BspTI CTTAAG 1 cut(s) 94
BsrDI GCAATG 2 cut(s) 413, 621
BssECI CCNNGG 1 cut(s) 636
BssMI GATC 3 cut(s) 31, 74, 244
BssNI GRCGYC 1 cut(s) 206
BssSI CACGAG 1 cut(s) 626
Bst2BI CACGAG 1 cut(s) 626
Bst4CI ACNGT 4 cut(s) 307, 365, 448, 574
BstACI GRCGYC 1 cut(s) 206
BstAFI CTTAAG 1 cut(s) 94
BstDEI CTNAG 1 cut(s) 481
BstF5I GGATG 1 cut(s) 464
BstKTI GATC 3 cut(s) 34, 77, 247
BstMAI GTCTC 1 cut(s) 629
BstMBI GATC 3 cut(s) 31, 74, 244
BstSCI CCNGG 2 cut(s) 43, 635
BstSFI CTRYAG 1 cut(s) 573
BstSLI GKGCMC 1 cut(s) 127
BstV1I GCAGC 3 cut(s) 170, 340, 476
BstV2I GAAGAC 2 cut(s) 201, 265
BstXI CCANNNNNNTGG 1 cut(s) 150
Bsu36I CCTNAGG 1 cut(s) 481
BsuRI GGCC 1 cut(s) 635
BtsCI GGATG 1 cut(s) 464
BtsIMutI CAGTG 2 cut(s) 84, 570
Cfr13I GGNCC 1 cut(s) 454
Csp6I GTAC 3 cut(s) 63, 260, 475
CviAII CATG 5 cut(s) 23, 128, 222, 236, 500
CviJI RGCY 9 cut(s) 29, 161, 253, 284, 353, 386, 489, 635, 641
CviKI_1 RGCY 9 cut(s) 29, 161, 253, 284, 353, 386, 489, 635, 641
CviQI GTAC 3 cut(s) 63, 260, 475
DdeI CTNAG 1 cut(s) 481
DpnI GATC 3 cut(s) 33, 76, 246
DpnII GATC 3 cut(s) 31, 74, 244
EaeI YGGCCR 1 cut(s) 633
Eco47I GGWCC 1 cut(s) 454
Eco81I CCTNAGG 1 cut(s) 481
FaeI CATG 5 cut(s) 26, 131, 225, 239, 503
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 1 cut(s) 2
FatI CATG 5 cut(s) 22, 127, 221, 235, 499
FauNDI CATATG 1 cut(s) 327
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 3 cut(s) 159, 354, 490
FokI GGATG 1 cut(s) 451
Fsp4HI GCNGC 3 cut(s) 159, 354, 490
FspBI CTAG 1 cut(s) 381
GluI GCNGC 3 cut(s) 159, 354, 490
HaeIII GGCC 1 cut(s) 635
HapII CCGG 2 cut(s) 44, 636
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 5 cut(s) 26, 131, 225, 239, 503
HinfI GANTC 4 cut(s) 5, 230, 331, 567
HpaII CCGG 2 cut(s) 44, 636
HphI GGTGA 3 cut(s) 132, 190, 313
Hpy166II GTNNAC 1 cut(s) 198
Hpy188III TCNNGA 2 cut(s) 400, 442
Hpy8I GTNNAC 1 cut(s) 198
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 4 cut(s) 307, 365, 448, 574
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 5 cut(s) 406, 492, 543, 597, 653
HpyF3I CTNAG 1 cut(s) 481
HpySE526I ACGT 1 cut(s) 206
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 5 cut(s) 26, 131, 225, 239, 503
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 3 cut(s) 31, 74, 244
LmnI GCTCC 1 cut(s) 47
LpnPI CCDG 5 cut(s) 57, 156, 303, 348, 649
Lsp1109I GCAGC 3 cut(s) 170, 340, 476
LweI GCATC 1 cut(s) 393
MaeI CTAG 1 cut(s) 381
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 2 cut(s) 301, 568
MalI GATC 3 cut(s) 33, 76, 246
MboI GATC 3 cut(s) 31, 74, 244
MboII GAAGA 2 cut(s) 201, 265
MfeI CAATTG 2 cut(s) 117, 420
MhlI GDGCHC 1 cut(s) 127
MluCI AATT 4 cut(s) 38, 117, 420, 533
MlyI GAGTC 3 cut(s) 14, 340, 576
MmeI TCCRAC 2 cut(s) 34, 544
MnlI CCTC 2 cut(s) 232, 305
MseI TTAA 2 cut(s) 95, 658
MslI CAYNNNNRTG 2 cut(s) 401, 548
MspCI CTTAAG 1 cut(s) 94
MspI CCGG 2 cut(s) 44, 636
MspR9I CCNGG 2 cut(s) 45, 637
MunI CAATTG 2 cut(s) 117, 420
Mva1269I GAATGC 1 cut(s) 578
NciI CCSGG 2 cut(s) 45, 637
NdeI CATATG 1 cut(s) 327
NdeII GATC 3 cut(s) 31, 74, 244
NlaIII CATG 5 cut(s) 26, 131, 225, 239, 503
NlaIV GGNNCC 2 cut(s) 124, 511
NmuCI GTSAC 2 cut(s) 301, 568
OliI CACNNNNGTG 1 cut(s) 548
PctI GAATGC 1 cut(s) 578
PfeI GAWTC 1 cut(s) 230
PkrI GCNGC 3 cut(s) 160, 355, 491
PleI GAGTC 3 cut(s) 13, 339, 575
PpsI GAGTC 3 cut(s) 13, 339, 575
PsiI TTATAA 1 cut(s) 371
PspN4I GGNNCC 2 cut(s) 124, 511
PspPI GGNCC 1 cut(s) 454
RsaI GTAC 3 cut(s) 64, 261, 476
RsaNI GTAC 3 cut(s) 63, 260, 475
RseI CAYNNNNRTG 2 cut(s) 401, 548
SaqAI TTAA 2 cut(s) 95, 658
SatI GCNGC 3 cut(s) 159, 354, 490
Sau3AI GATC 3 cut(s) 31, 74, 244
Sau96I GGNCC 1 cut(s) 454
ScaI AGTACT 1 cut(s) 476
SchI GAGTC 3 cut(s) 14, 340, 576
ScrFI CCNGG 2 cut(s) 45, 637
SduI GDGCHC 1 cut(s) 127
SfaNI GCATC 1 cut(s) 393
SfcI CTRYAG 1 cut(s) 573
SinI GGWCC 1 cut(s) 454
SmiMI CAYNNNNRTG 2 cut(s) 401, 548
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 4 cut(s) 38, 117, 420, 533
SsiI CCGC 1 cut(s) 269
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 2 cut(s) 43, 635
TaaI ACNGT 4 cut(s) 307, 365, 448, 574
TaiI ACGT 1 cut(s) 209
TaqI TCGA 2 cut(s) 8, 582
TasI AATT 4 cut(s) 38, 117, 420, 533
TatI WGTACW 1 cut(s) 474
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 2 cut(s) 95, 658
Tru9I TTAA 2 cut(s) 95, 658
TscAI CASTG 2 cut(s) 84, 577
TseFI GTSAC 2 cut(s) 301, 568
TseI GCWGC 3 cut(s) 158, 353, 489
Tsp45I GTSAC 2 cut(s) 301, 568
TspDTI ATGAA 4 cut(s) 39, 144, 265, 337
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 2 cut(s) 84, 577
Vha464I CTTAAG 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 454
XapI RAATTY 1 cut(s) 533
XspI CTAG 1 cut(s) 381
ZraI GACGTC 1 cut(s) 207
ZrmI AGTACT 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.