Rmu_sc0005711.1_g000003

Bacterial extracellular solute-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005711.1
OG0011078
Physical Location & Seq
Forward (+)
15555 .. 19729
4175 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1617 bp
atggctactgctccattgctgccgtcacgcatctgtttccctttggatccctcatgttcccaattccaaagccctaaccttaatccctccttccctctccgccgtcgctctaaccatactcatagcctcactctcacactcgtctcttctgcccacactctcactccgccggagccgccgccgccttctacttccaccaccgtccctcacaccttgcttcggttcgccgcctccgccgccctccttctcgggattgggctcggatgctgcgtcgcctccgcccgctcttctcctctagcccagcctctgccggtgctacaggatcaagctacggatgatgacaatgtagaagcggcgtttggaacttggaagtccaagacattcgctttgactgtgcccttgacagttgcagctctacgcggctctgtacctccttcatggattagggattttatgcagtctcaaggaaaaagatcaaagcttcaactcaagtctcatgcaagtctcgagagcgtgttttcggatctctcaagagacttgagcaaaggcgatgttcggccttcttctgttgcggctgctgatgtagttagtattggggactcttggctcagtcatgccattaacaagggtctaattgagccaattcaaggggtagaagaccaggattggtataaaggattgagtgacagatggaaagtatatctacgcaggaactctgaggggagaattgaccctgatggtaaaatatgggctgctccatatcggtggggctgcatggtgatagcatacaaaaaaactaaatttagaaagcataacttggctccaatatcggactgggcagatttatggcgacctgaacttgcaggaaggatttcaatggttgattcttctagagaggttatcggtgcagttctgaagcatatgggaacctcctacaacacacaagatatccatttgcaagttgatggtgggaaagatgctgtaaggcaaaatctggctttacttggaaaacaggtccaactatttgacagtgtacactatttgaaagcatttgcggttggagatgtgtgggtggctgtcgggtggagcagtgatgttcttcctgttgccaaacgcatgtctgatgttgcagtaattgttccgaaatctggaactagtatatgggcagatatgtgggcaacccctgctgtctctcgacttgaaacaaatcgaattggggggcgagttagagggccatctccactcatccatcagtggatagacttctgcttgcaaactgctagagcactcccttttaagcaggaggtaatcccaggcgcctccccctctgcacttgatagtgccccggctgaaaatttagaagagttcaccaagggtaagccaagactggatacaaacctcattgtgggagtaccgccacctgagattttggcaaggtgtgagtttttggagccattatctgactccacactatcagattatcaatcgctaatagatagtatgcagaaatctaaacatggtttggtccctagtataaattacaatatcttatcattgattcaaaatttgtggttgaagctgcattcaaataataacaagagcttgaagcaagtttag

Protein Analysis

538

Amino Acids

59.0

Weight (kDa)

9.02

Isoelectric Point (pI)

54.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP_bac_1 PF01547 176 - 391 8.9e-06 Bacterial extracellular solute-binding protein
SBP_bac_8 PF13416 191 - 398 1.5e-17 Bacterial extracellular solute-binding protein
SBP_bac_11 PF13531 192 - 368 3.9e-06 Bacterial extracellular solute-binding protein
SBP_bac_6 PF13343 193 - 451 2.2e-23 Bacterial extracellular solute-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011078)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31410
fragaria_vesca FvH4_3g41920 FvH4_3g41920 FvH4_3g41920
malus_domestica MD07G1026800.v1.1
prunus_persica Prupe.2G060600_v2.0.a1 Prupe.2G060600_v2.0.a1
pyrus_communis pycom07g02020 pycom07g02060
rosa_chinensis RchiOBHm_Chr1g0331171
rosa_laevigata RLG00000029748
rosa_multiflora Rmu_sc0005711.1_g000003
rosa_roxburghii Rroxscaffold_4G00319290
rosa_rugosa Rorug01G0091900 Rorug01G0092000
rosa_samantha Rh1AG116000 Rh1BG087700 Rh1CG111500 Rh1DG120400
rosa_wichuraiana Rw1G009320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1316
AccBSI CCGCTC 1 cut(s) 285
AccII CGCG 1 cut(s) 420
AclWI GGATC 4 cut(s) 41, 54, 330, 531
AcsI RAATTY 3 cut(s) 800, 1354, 1564
AcuI CTGAAG 1 cut(s) 935
AcyI GRCGYC 1 cut(s) 1317
AfaI GTAC 3 cut(s) 429, 1035, 1413
AfiI CCNNNNNNNGG 4 cut(s) 219, 647, 1148, 1405
AgsI TTSAA 9 cut(s) 485, 647, 876, 1045, 1202, 1562, 1576, 1587, 1606
AhlI ACTAGT 1 cut(s) 1154
AjnI CCWGG 2 cut(s) 660, 1312
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AluBI AGCT 5 cut(s) 329, 413, 481, 1579, 1602
AluI AGCT 5 cut(s) 329, 413, 481, 1579, 1602
Alw21I GWGCWC 1 cut(s) 1288
Alw26I GTCTC 6 cut(s) 148, 465, 498, 509, 528, 1195
AlwI GGATC 4 cut(s) 41, 54, 330, 531
AlwNI CAGNNNCTG 1 cut(s) 307
Ama87I CYCGRG 2 cut(s) 248, 506
AoxI GGCC 2 cut(s) 557, 1232
ApeKI GCWGC 7 cut(s) 19, 267, 410, 575, 752, 771, 1579
ApoI RAATTY 3 cut(s) 800, 1354, 1564
ArsI GACNNNNNNTTYG 2 cut(s) 370, 402
Asp700I GAANNNNTTC 1 cut(s) 871
AspLEI GCGC 1 cut(s) 1319
AspS9I GGNCC 3 cut(s) 1015, 1232, 1525
AsuC2I CCSGG 1 cut(s) 1346
AsuHPI GGTGA 2 cut(s) 790, 1360
AvaI CYCGRG 2 cut(s) 248, 506
AvaII GGWCC 2 cut(s) 1015, 1525
BaeGI GKGCMC 2 cut(s) 399, 1345
BamHI GGATCC 1 cut(s) 46
BanI GGYRCC 1 cut(s) 1316
BanII GRGCYC 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 663
Bbv12I GWGCWC 1 cut(s) 1288
BbvI GCAGC 7 cut(s) 6, 254, 422, 562, 739, 758, 1566
BccI CCATC 5 cut(s) 684, 731, 959, 1243, 1257
BceAI ACGGC 2 cut(s) 7, 87
BciT130I CCWGG 2 cut(s) 662, 1314
BciVI GTATCC 1 cut(s) 1384
BcnI CCSGG 1 cut(s) 1346
BcoDI GTCTC 6 cut(s) 148, 465, 498, 509, 528, 1195
BcuI ACTAGT 1 cut(s) 1154
BfaI CTAG 5 cut(s) 296, 891, 1155, 1281, 1530
BfmI CTRYAG 1 cut(s) 317
BfoI RGCGCY 1 cut(s) 1320
BfuI GTATCC 1 cut(s) 1384
Bme1390I CCNGG 3 cut(s) 662, 1314, 1346
Bme18I GGWCC 2 cut(s) 1015, 1525
BmeT110I CYCGRG 2 cut(s) 248, 506
BmgT120I GGNCC 3 cut(s) 1015, 1232, 1525
BmiI GGNNCC 7 cut(s) 48, 174, 822, 928, 1318, 1452, 1527
BmrFI CCNGG 3 cut(s) 662, 1314, 1346
BmrI ACTGGG 1 cut(s) 844
BmsI GCATC 3 cut(s) 39, 254, 967
BmuI ACTGGG 1 cut(s) 844
BpiI GAAGAC 1 cut(s) 663
BpuEI CTTGAG 4 cut(s) 447, 473, 514, 559
BpuMI CCSGG 1 cut(s) 1346
BsaHI GRCGYC 1 cut(s) 1317
BsaJI CCNNGG 3 cut(s) 1312, 1344, 1371
BsaXI ACNNNNNCTCC 2 cut(s) 148, 178
Bsc4I CCNNNNNNNGG 4 cut(s) 219, 647, 1148, 1405
Bse118I RCCGGY 1 cut(s) 310
Bse1I ACTGG 2 cut(s) 839, 1392
Bse3DI GCAATG 1 cut(s) 14
BseBI CCWGG 2 cut(s) 662, 1314
BseDI CCNNGG 3 cut(s) 1312, 1344, 1371
BseGI GGATG 3 cut(s) 269, 340, 1245
BseLI CCNNNNNNNGG 4 cut(s) 219, 647, 1148, 1405
BseMI GCAATG 1 cut(s) 14
BseMII CTCAG 3 cut(s) 622, 708, 1413
BseNI ACTGG 2 cut(s) 839, 1392
BseRI GAGGAG 1 cut(s) 282
BseSI GKGCMC 2 cut(s) 399, 1345
BseXI GCAGC 7 cut(s) 6, 254, 422, 562, 739, 758, 1566
BseYI CCCAGC 1 cut(s) 300
BsgI GTGCAG 2 cut(s) 927, 1314
Bsh1236I CGCG 1 cut(s) 420
BshFI GGCC 2 cut(s) 559, 1234
BshNI GGYRCC 1 cut(s) 1316
BsiHKAI GWGCWC 1 cut(s) 1288
BsiHKCI CYCGRG 2 cut(s) 248, 506
BsiSI CCGG 3 cut(s) 170, 311, 1346
BslFI GGGAC 3 cut(s) 188, 611, 1511
BslI CCNNNNNNNGG 4 cut(s) 219, 647, 1148, 1405
BsmAI GTCTC 6 cut(s) 148, 465, 498, 509, 528, 1195
BsmBI CGTCTC 1 cut(s) 148
BsmFI GGGAC 3 cut(s) 188, 611, 1511
BsmI GAATGC 1 cut(s) 1582
BsnI GGCC 2 cut(s) 559, 1234
BsoBI CYCGRG 2 cut(s) 248, 506
Bsp1286I GDGCHC 4 cut(s) 261, 399, 1288, 1345
Bsp1407I TGTACA 1 cut(s) 1033
Bsp143I GATC 4 cut(s) 46, 322, 473, 523
BspANI GGCC 2 cut(s) 559, 1234
BspCNI CTCAG 3 cut(s) 621, 709, 1414
BspFNI CGCG 1 cut(s) 420
BspLI GGNNCC 7 cut(s) 48, 174, 822, 928, 1318, 1452, 1527
BspPI GGATC 4 cut(s) 41, 54, 330, 531
BspQI GCTCTTC 1 cut(s) 292
BspT107I GGYRCC 1 cut(s) 1316
BsrBI CCGCTC 1 cut(s) 285
BsrDI GCAATG 1 cut(s) 14
BsrFI RCCGGY 1 cut(s) 310
BsrGI TGTACA 1 cut(s) 1033
BsrI ACTGG 2 cut(s) 839, 1392
BssAI RCCGGY 1 cut(s) 310
BssECI CCNNGG 3 cut(s) 1312, 1344, 1371
BssMI GATC 4 cut(s) 46, 322, 473, 523
BssNI GRCGYC 1 cut(s) 1317
BssT1I CCWWGG 1 cut(s) 1371
Bst2UI CCWGG 2 cut(s) 662, 1314
Bst4CI ACNGT 4 cut(s) 202, 394, 406, 1031
Bst6I CTCTTC 3 cut(s) 151, 292, 1356
BstACI GRCGYC 1 cut(s) 1317
BstAPI GCANNNNNTGC 1 cut(s) 1184
BstAUI TGTACA 1 cut(s) 1033
BstC8I GCNNGC 2 cut(s) 283, 1271
BstDEI CTNAG 3 cut(s) 608, 717, 1422
BstF5I GGATG 3 cut(s) 269, 340, 1245
BstFNI CGCG 1 cut(s) 420
BstH2I RGCGCY 1 cut(s) 1320
BstHHI GCGC 1 cut(s) 1319
BstKTI GATC 4 cut(s) 49, 325, 476, 526
BstMAI GTCTC 6 cut(s) 148, 465, 498, 509, 528, 1195
BstMBI GATC 4 cut(s) 46, 322, 473, 523
BstMWI GCNNNNNNNGC 5 cut(s) 175, 181, 233, 236, 1184
BstNI CCWGG 2 cut(s) 662, 1314
BstNSI RCATGY 1 cut(s) 1120
BstSCI CCNGG 3 cut(s) 660, 1312, 1344
BstSFI CTRYAG 1 cut(s) 317
BstSLI GKGCMC 2 cut(s) 399, 1345
BstUI CGCG 1 cut(s) 420
BstV1I GCAGC 7 cut(s) 6, 254, 422, 562, 739, 758, 1566
BstV2I GAAGAC 1 cut(s) 663
BstX2I RGATCY 2 cut(s) 46, 523
BstXI CCANNNNNNTGG 1 cut(s) 765
BstYI RGATCY 2 cut(s) 46, 523
BsuI GTATCC 1 cut(s) 1384
BsuRI GGCC 2 cut(s) 559, 1234
BtgZI GCGATG 1 cut(s) 564
BtsCI GGATG 3 cut(s) 269, 340, 1245
BtsI GCAGTG 1 cut(s) 1096
BtsIMutI CAGTG 3 cut(s) 1036, 1096, 1259
Cac8I GCNNGC 2 cut(s) 283, 1271
CaiI CAGNNNCTG 1 cut(s) 307
CfoI GCGC 1 cut(s) 1319
Cfr10I RCCGGY 1 cut(s) 310
Cfr13I GGNCC 3 cut(s) 1015, 1232, 1525
CseI GACGC 1 cut(s) 259
Csp6I GTAC 3 cut(s) 428, 1034, 1412
CspCI CAANNNNNGTGG 2 cut(s) 1550, 1585
CviAII CATG 7 cut(s) 54, 438, 497, 614, 775, 1117, 1517
CviQI GTAC 3 cut(s) 428, 1034, 1412
DdeI CTNAG 3 cut(s) 608, 717, 1422
DinI GGCGCC 1 cut(s) 1318
DpnI GATC 4 cut(s) 48, 324, 475, 525
DpnII GATC 4 cut(s) 46, 322, 473, 523
Eam1104I CTCTTC 3 cut(s) 151, 292, 1356
EarI CTCTTC 3 cut(s) 151, 292, 1356
EciI GGCGGA 4 cut(s) 89, 156, 223, 268
Eco130I CCWWGG 1 cut(s) 1371
Eco24I GRGCYC 1 cut(s) 261
Eco32I GATATC 1 cut(s) 949
Eco47I GGWCC 2 cut(s) 1015, 1525
Eco57I CTGAAG 1 cut(s) 935
Eco88I CYCGRG 2 cut(s) 248, 506
EcoRII CCWGG 2 cut(s) 660, 1312
EcoRV GATATC 1 cut(s) 949
EcoT14I CCWWGG 1 cut(s) 1371
EcoT38I GRGCYC 1 cut(s) 261
EgeI GGCGCC 1 cut(s) 1318
EheI GGCGCC 1 cut(s) 1318
ErhI CCWWGG 1 cut(s) 1371
Esp3I CGTCTC 1 cut(s) 148
FaeI CATG 7 cut(s) 57, 441, 500, 617, 778, 1120, 1520
FalI AAGNNNNNCTT 2 cut(s) 800, 832
FaqI GGGAC 3 cut(s) 188, 611, 1511
FatI CATG 7 cut(s) 53, 437, 496, 613, 774, 1116, 1516
FauI CCCGC 1 cut(s) 290
FauNDI CATATG 1 cut(s) 921
FokI GGATG 3 cut(s) 276, 347, 1232
FriOI GRGCYC 1 cut(s) 261
FspBI CTAG 5 cut(s) 296, 891, 1155, 1281, 1530
GlaI GCGC 1 cut(s) 1318
GsaI CCCAGC 1 cut(s) 304
HaeII RGCGCY 1 cut(s) 1320
HaeIII GGCC 2 cut(s) 559, 1234
HapII CCGG 3 cut(s) 170, 311, 1346
HgaI GACGC 1 cut(s) 259
HhaI GCGC 1 cut(s) 1319
Hin1I GRCGYC 1 cut(s) 1317
Hin1II CATG 7 cut(s) 57, 441, 500, 617, 778, 1120, 1520
Hin6I GCGC 1 cut(s) 1317
HinP1I GCGC 1 cut(s) 1317
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 4 cut(s) 599, 884, 1463, 1558
HpaII CCGG 3 cut(s) 170, 311, 1346
HphI GGTGA 2 cut(s) 790, 1360
Hpy166II GTNNAC 3 cut(s) 1034, 1036, 1368
Hpy188I TCNGA 9 cut(s) 263, 523, 718, 832, 915, 1123, 1143, 1462, 1477
Hpy188III TCNNGA 7 cut(s) 250, 506, 508, 531, 891, 1149, 1194
Hpy8I GTNNAC 3 cut(s) 1034, 1036, 1368
Hpy99I CGWCG 2 cut(s) 108, 275
HpyAV CCTTC 6 cut(s) 100, 195, 254, 444, 570, 861
HpyCH4III ACNGT 4 cut(s) 202, 394, 406, 1031
HpyF10VI GCNNNNNNNGC 5 cut(s) 175, 181, 233, 236, 1184
HpyF3I CTNAG 3 cut(s) 608, 717, 1422
Hsp92I GRCGYC 1 cut(s) 1317
Hsp92II CATG 7 cut(s) 57, 441, 500, 617, 778, 1120, 1520
HspAI GCGC 1 cut(s) 1317
KasI GGCGCC 1 cut(s) 1316
Kzo9I GATC 4 cut(s) 46, 322, 473, 523
LguI GCTCTTC 1 cut(s) 292
LmnI GCTCC 6 cut(s) 16, 172, 760, 826, 1086, 1450
Lsp1109I GCAGC 7 cut(s) 6, 254, 422, 562, 739, 758, 1566
LweI GCATC 3 cut(s) 39, 254, 967
MaeI CTAG 5 cut(s) 296, 891, 1155, 1281, 1530
MaeIII GTNAC 2 cut(s) 24, 683
MalI GATC 4 cut(s) 48, 324, 475, 525
MbiI CCGCTC 1 cut(s) 285
MboI GATC 4 cut(s) 46, 322, 473, 523
MboII GAAGA 7 cut(s) 138, 279, 555, 668, 879, 1091, 1373
MflI RGATCY 2 cut(s) 46, 523
MhlI GDGCHC 4 cut(s) 261, 399, 1288, 1345
Mly113I GGCGCC 1 cut(s) 1317
MlyI GAGTC 2 cut(s) 593, 1457
MmeI TCCRAC 2 cut(s) 1039, 1042
MroXI GAANNNNTTC 1 cut(s) 871
MseI TTAA 3 cut(s) 81, 621, 1296
MslI CAYNNNNRTG 1 cut(s) 763
MspI CCGG 3 cut(s) 170, 311, 1346
MspR9I CCNGG 3 cut(s) 662, 1314, 1346
Mva1269I GAATGC 1 cut(s) 1582
MvaI CCWGG 2 cut(s) 662, 1314
MvnI CGCG 1 cut(s) 420
MwoI GCNNNNNNNGC 5 cut(s) 175, 181, 233, 236, 1184
NarI GGCGCC 1 cut(s) 1317
NciI CCSGG 1 cut(s) 1346
NdeI CATATG 1 cut(s) 921
NdeII GATC 4 cut(s) 46, 322, 473, 523
NlaIII CATG 7 cut(s) 57, 441, 500, 617, 778, 1120, 1520
NlaIV GGNNCC 7 cut(s) 48, 174, 822, 928, 1318, 1452, 1527
NmuCI GTSAC 2 cut(s) 24, 683
NspI RCATGY 1 cut(s) 1120
PaeR7I CTCGAG 1 cut(s) 506
PciSI GCTCTTC 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 1582
PdmI GAANNNNTTC 1 cut(s) 871
PfeI GAWTC 2 cut(s) 884, 1558
PleI GAGTC 2 cut(s) 593, 1457
PluTI GGCGCC 1 cut(s) 1320
PpsI GAGTC 2 cut(s) 593, 1457
Psp6I CCWGG 2 cut(s) 660, 1312
PspFI CCCAGC 1 cut(s) 300
PspGI CCWGG 2 cut(s) 660, 1312
PspN4I GGNNCC 7 cut(s) 48, 174, 822, 928, 1318, 1452, 1527
PspPI GGNCC 3 cut(s) 1015, 1232, 1525
PstNI CAGNNNCTG 1 cut(s) 307
PsuI RGATCY 2 cut(s) 46, 523
RsaI GTAC 3 cut(s) 429, 1035, 1413
RsaNI GTAC 3 cut(s) 428, 1034, 1412
RseI CAYNNNNRTG 1 cut(s) 763
SapI GCTCTTC 1 cut(s) 292
SaqAI TTAA 3 cut(s) 81, 621, 1296
Sau3AI GATC 4 cut(s) 46, 322, 473, 523
Sau96I GGNCC 3 cut(s) 1015, 1232, 1525
SchI GAGTC 2 cut(s) 593, 1457
ScrFI CCNGG 3 cut(s) 662, 1314, 1346
SduI GDGCHC 4 cut(s) 261, 399, 1288, 1345
SfaNI GCATC 3 cut(s) 39, 254, 967
SfcI CTRYAG 1 cut(s) 317
SfoI GGCGCC 1 cut(s) 1318
Sfr274I CTCGAG 1 cut(s) 506
SinI GGWCC 2 cut(s) 1015, 1525
SlaI CTCGAG 1 cut(s) 506
SmiMI CAYNNNNRTG 1 cut(s) 763
SmlI CTYRAG 5 cut(s) 462, 488, 506, 529, 538
SmoI CTYRAG 5 cut(s) 462, 488, 506, 529, 538
SpeI ACTAGT 1 cut(s) 1154
SspDI GGCGCC 1 cut(s) 1316
SspMI CTAG 5 cut(s) 296, 891, 1155, 1281, 1530
StyD4I CCNGG 3 cut(s) 660, 1312, 1344
StyI CCWWGG 1 cut(s) 1371
TaaI ACNGT 4 cut(s) 202, 394, 406, 1031
TaqI TCGA 3 cut(s) 507, 1195, 1210
TatI WGTACW 1 cut(s) 1033
TauI GCSGC 8 cut(s) 178, 181, 184, 230, 239, 356, 423, 575
TfiI GAWTC 2 cut(s) 884, 1558
Tru1I TTAA 3 cut(s) 81, 621, 1296
Tru9I TTAA 3 cut(s) 81, 621, 1296
TscAI CASTG 3 cut(s) 1036, 1096, 1259
TseFI GTSAC 2 cut(s) 24, 683
TseI GCWGC 7 cut(s) 19, 267, 410, 575, 752, 771, 1579
Tsp45I GTSAC 2 cut(s) 24, 683
TspDTI ATGAA 1 cut(s) 426
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 3 cut(s) 1036, 1096, 1259
VpaK11BI GGWCC 2 cut(s) 1015, 1525
XapI RAATTY 3 cut(s) 800, 1354, 1564
XbaI TCTAGA 1 cut(s) 890
XceI RCATGY 1 cut(s) 1120
XcmI CCANNNNNNNNNTGG 1 cut(s) 831
XhoI CTCGAG 1 cut(s) 506
XmnI GAANNNNTTC 1 cut(s) 871
XspI CTAG 5 cut(s) 296, 891, 1155, 1281, 1530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.