Rh1BG087700

Bacterial extracellular solute-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0011078
Physical Location & Seq
Forward (+)
13909115 .. 13913502
4388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG087700.1

Sequence Viewer

Length: 1626 bp
ATGGCTACTGCTGCTACTGCTCCATTGCTGCCGTCACGCATCTGGTTCCACTTGGATCCTTCATGTTCCCAATTCCAAAGCCCTAACCTTAATCCCTCCTTCCCTCTCCGCCGTCGCTCTAACCATACCCATAGGCTCACTCTCACACTCGTCTCTTCTGCCCGCACTCTCACTCCGCCGGAGCCGCCGCTGCCTACTACCTCCACCACCGTCCCTCACACCTTGCTTCGGTTCGCCGCCTCTGCCGCCCTCCTTCTCGGGATTGGGCTCGGATGCTGCGTCGCCTCCGCCCGCTCTTCTCCTCTAGCCCAGCCTCTGCCGGCGCTACAGGATCAAGCTACGGATGATGACAATTTAGAAGCGGCATTTGGAACTTGGAAGTCCAAGACATTCGCTTTGACTGTGCCCTTGACAGTTGCTGCTCTACGTGGCTCTGTACCTCCTTCATGGATTAAGGATTTTATGCAGTCTCAAGGAAAAAGATCAAAGCTTCAACTCAAGTCTCATGCAAGTCTCGAGAGCGTATTTTCGGATCTCTCAAGAGACTTGAGCAAGGGCGATGTTCGGCCTTCTTCTGTTGCGGCTGCTGATGTAGTTAGTATTGGGGACTCTTGGCTCAGTCATGCCATTAACAAGGGTCTAATTGAGCCAATTCAAGGGGTAGAAGACCAGGATTGGTATAAAGGATTGAGTGACAGATGGAAAGTATATCTACGCAGGAACTCTGAGGGGAGAATTGACCCTGATGGTAAAATATGGGCTGCTCCATATCGGTGGGGCTGCATGGAGATAGCATACAAAAAAACTAAATTTAGAAAGCATAACTTGGCTCCTATATTGGACTGGGCAGATTTATGGCGACCTGAACTTGCAGGAAGGATTTCAATGGTTGATTCTTCTAGAGAGGTTATCGGTGCAGTTCTGAAGCATATGGGAACCTCCTACAACACACAAGATATCCATTTGCAAGTTGATGGTGGGAAAGATGCTGTAAGGCAAAATCTGGCTTTACTTGGAAAACAGGTCCGACTATTTGACAGTGTACACTATTTGAAAGCATTTGCGGTTGGAGATGTGTGGGTGGCTGTTGGGTGGAGCAGTGATGTTCTTCCTGTTGCCAAACGCATGTCTGATGTTGCAGTAATTGTTCCGAAATCTGGAACTAGTATATGGGCAGATATGTGGGCAACCCCTGCTGTCTCTCGACTTGAAACAAATCGAATTGGGGGGCGAGTTAGAGGGCCATCTCCACTCATCCATCAGTGGATAGACTTCTGCTTGCAAACTGCTAGAGCACTGCCTTTTAAGCAGGAGGTAATCCCAGGCGCCTCCCCCTCTGCACTTGATAGTGCCTCGGCTGAAAATTTAGAAGAGTTCACTAATGGTAAACCAAGACTGGACACAAACCTCATTGCGGGAGTACCACCACCTGAGATTTTGGCAAGATGTGAGTTTTTGGAGCCATTATCTGACTCCACAATATCAGATTATCAATTGCTAATAGATAGTATGCAGGAATCTAAACATGGTTTGGTCCCTAGTATAAATTACAATATCTTATCATTGATTCAAAATTTGTGGTTGAAGCTGCATTCAAATAATAACAAGAGCTTGAAGCAAGTTTAG

Protein Analysis

541

Amino Acids

59.5

Weight (kDa)

8.98

Isoelectric Point (pI)

52.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP_bac_8 PF13416 194 - 401 1.3e-15 Bacterial extracellular solute-binding protein
SBP_bac_6 PF13343 196 - 451 6.6e-21 Bacterial extracellular solute-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011078)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31410
fragaria_vesca FvH4_3g41920 FvH4_3g41920 FvH4_3g41920
malus_domestica MD07G1026800.v1.1
prunus_persica Prupe.2G060600_v2.0.a1 Prupe.2G060600_v2.0.a1
pyrus_communis pycom07g02020 pycom07g02060
rosa_chinensis RchiOBHm_Chr1g0331171
rosa_laevigata RLG00000029748
rosa_multiflora Rmu_sc0005711.1_g000003
rosa_roxburghii Rroxscaffold_4G00319290
rosa_rugosa Rorug01G0091900 Rorug01G0092000
rosa_samantha Rh1AG116000 Rh1BG087700 Rh1CG111500 Rh1DG120400
rosa_wichuraiana Rw1G009320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1325
AccBSI CCGCTC 1 cut(s) 294
AclWI GGATC 4 cut(s) 50, 63, 339, 540
AcsI RAATTY 3 cut(s) 809, 1363, 1573
AcuI CTGAAG 1 cut(s) 944
AcyI GRCGYC 1 cut(s) 1326
AfaI GTAC 3 cut(s) 438, 1044, 1422
AfiI CCNNNNNNNGG 4 cut(s) 228, 656, 1157, 1414
AgsI TTSAA 9 cut(s) 494, 656, 885, 1054, 1211, 1571, 1585, 1596, 1615
AhlI ACTAGT 1 cut(s) 1163
AjnI CCWGG 2 cut(s) 669, 1321
AjuI GAANNNNNNNTTGG 2 cut(s) 351, 383
AluBI AGCT 4 cut(s) 338, 490, 1588, 1611
AluI AGCT 4 cut(s) 338, 490, 1588, 1611
Alw21I GWGCWC 1 cut(s) 1297
Alw26I GTCTC 6 cut(s) 157, 474, 507, 518, 537, 1204
AlwI GGATC 4 cut(s) 50, 63, 339, 540
AlwNI CAGNNNCTG 2 cut(s) 316, 419
Ama87I CYCGRG 2 cut(s) 257, 515
AoxI GGCC 2 cut(s) 566, 1241
ApeKI GCWGC 9 cut(s) 11, 28, 190, 276, 419, 584, 761, 780, 1588
ApoI RAATTY 3 cut(s) 809, 1363, 1573
ArsI GACNNNNNNTTYG 2 cut(s) 379, 411
Asp700I GAANNNNTTC 1 cut(s) 880
AspLEI GCGC 2 cut(s) 325, 1328
AspS9I GGNCC 3 cut(s) 1024, 1241, 1534
AvaI CYCGRG 2 cut(s) 257, 515
AvaII GGWCC 2 cut(s) 1024, 1534
BaeGI GKGCMC 1 cut(s) 408
BamHI GGATCC 1 cut(s) 55
BanI GGYRCC 1 cut(s) 1325
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 672
Bbv12I GWGCWC 1 cut(s) 1297
BbvI GCAGC 8 cut(s) 15, 177, 263, 406, 571, 748, 767, 1575
BccI CCATC 5 cut(s) 693, 740, 968, 1252, 1266
BceAI ACGGC 2 cut(s) 16, 96
BciT130I CCWGG 2 cut(s) 671, 1323
BcoDI GTCTC 6 cut(s) 157, 474, 507, 518, 537, 1204
BcuI ACTAGT 1 cut(s) 1163
BfaI CTAG 5 cut(s) 305, 900, 1164, 1290, 1539
BfmI CTRYAG 1 cut(s) 326
BfoI RGCGCY 2 cut(s) 326, 1329
Bme1390I CCNGG 2 cut(s) 671, 1323
Bme18I GGWCC 2 cut(s) 1024, 1534
BmeT110I CYCGRG 2 cut(s) 257, 515
BmgT120I GGNCC 3 cut(s) 1024, 1241, 1534
BmiI GGNNCC 8 cut(s) 47, 57, 183, 831, 937, 1327, 1461, 1536
BmrFI CCNGG 2 cut(s) 671, 1323
BmrI ACTGGG 1 cut(s) 853
BmsI GCATC 3 cut(s) 48, 263, 976
BmuI ACTGGG 1 cut(s) 853
BpiI GAAGAC 1 cut(s) 672
BpuEI CTTGAG 4 cut(s) 456, 482, 523, 568
BsaAI YACGTR 1 cut(s) 428
BsaHI GRCGYC 1 cut(s) 1326
BsaJI CCNNGG 2 cut(s) 1321, 1353
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 656, 1157, 1414
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 2 cut(s) 848, 1401
Bse3DI GCAATG 2 cut(s) 23, 1410
BseBI CCWGG 2 cut(s) 671, 1323
BseDI CCNNGG 2 cut(s) 1321, 1353
BseGI GGATG 3 cut(s) 278, 349, 1254
BseLI CCNNNNNNNGG 4 cut(s) 228, 656, 1157, 1414
BseMI GCAATG 2 cut(s) 23, 1410
BseMII CTCAG 3 cut(s) 631, 717, 1422
BseNI ACTGG 2 cut(s) 848, 1401
BseRI GAGGAG 1 cut(s) 291
BseSI GKGCMC 1 cut(s) 408
BseXI GCAGC 8 cut(s) 15, 177, 263, 406, 571, 748, 767, 1575
BseYI CCCAGC 1 cut(s) 309
BsgI GTGCAG 2 cut(s) 936, 1323
BshFI GGCC 2 cut(s) 568, 1243
BshNI GGYRCC 1 cut(s) 1325
BsiHKAI GWGCWC 1 cut(s) 1297
BsiHKCI CYCGRG 2 cut(s) 257, 515
BsiSI CCGG 2 cut(s) 179, 320
BslFI GGGAC 3 cut(s) 197, 620, 1520
BslI CCNNNNNNNGG 4 cut(s) 228, 656, 1157, 1414
BsmAI GTCTC 6 cut(s) 157, 474, 507, 518, 537, 1204
BsmBI CGTCTC 1 cut(s) 157
BsmFI GGGAC 3 cut(s) 197, 620, 1520
BsmI GAATGC 1 cut(s) 1591
BsnI GGCC 2 cut(s) 568, 1243
BsoBI CYCGRG 2 cut(s) 257, 515
Bsp1286I GDGCHC 3 cut(s) 270, 408, 1297
Bsp1407I TGTACA 1 cut(s) 1042
Bsp143I GATC 4 cut(s) 55, 331, 482, 532
BspANI GGCC 2 cut(s) 568, 1243
BspCNI CTCAG 3 cut(s) 630, 718, 1423
BspLI GGNNCC 8 cut(s) 47, 57, 183, 831, 937, 1327, 1461, 1536
BspPI GGATC 4 cut(s) 50, 63, 339, 540
BspQI GCTCTTC 1 cut(s) 301
BspT107I GGYRCC 1 cut(s) 1325
BsrBI CCGCTC 1 cut(s) 294
BsrDI GCAATG 2 cut(s) 23, 1410
BsrFI RCCGGY 1 cut(s) 319
BsrGI TGTACA 1 cut(s) 1042
BsrI ACTGG 2 cut(s) 848, 1401
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 2 cut(s) 1321, 1353
BssMI GATC 4 cut(s) 55, 331, 482, 532
BssNI GRCGYC 1 cut(s) 1326
Bst2UI CCWGG 2 cut(s) 671, 1323
Bst4CI ACNGT 4 cut(s) 211, 403, 415, 1040
Bst6I CTCTTC 3 cut(s) 160, 301, 1365
BstACI GRCGYC 1 cut(s) 1326
BstAPI GCANNNNNTGC 1 cut(s) 1193
BstAUI TGTACA 1 cut(s) 1042
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 4 cut(s) 163, 292, 321, 1280
BstDEI CTNAG 3 cut(s) 617, 726, 1431
BstF5I GGATG 3 cut(s) 278, 349, 1254
BstH2I RGCGCY 2 cut(s) 326, 1329
BstHHI GCGC 2 cut(s) 325, 1328
BstKTI GATC 4 cut(s) 58, 334, 485, 535
BstMAI GTCTC 6 cut(s) 157, 474, 507, 518, 537, 1204
BstMBI GATC 4 cut(s) 55, 331, 482, 532
BstMWI GCNNNNNNNGC 8 cut(s) 11, 17, 184, 190, 242, 245, 1193, 1306
BstNI CCWGG 2 cut(s) 671, 1323
BstNSI RCATGY 1 cut(s) 1129
BstSCI CCNGG 2 cut(s) 669, 1321
BstSFI CTRYAG 1 cut(s) 326
BstSLI GKGCMC 1 cut(s) 408
BstV1I GCAGC 8 cut(s) 15, 177, 263, 406, 571, 748, 767, 1575
BstV2I GAAGAC 1 cut(s) 672
BstX2I RGATCY 2 cut(s) 55, 532
BstXI CCANNNNNNTGG 1 cut(s) 774
BstYI RGATCY 2 cut(s) 55, 532
BsuRI GGCC 2 cut(s) 568, 1243
BtgZI GCGATG 1 cut(s) 573
BtsCI GGATG 3 cut(s) 278, 349, 1254
BtsI GCAGTG 2 cut(s) 1105, 1295
BtsIMutI CAGTG 4 cut(s) 1045, 1105, 1268, 1295
Cac8I GCNNGC 4 cut(s) 163, 292, 321, 1280
CaiI CAGNNNCTG 2 cut(s) 316, 419
CfoI GCGC 2 cut(s) 325, 1328
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 3 cut(s) 1024, 1241, 1534
CseI GACGC 1 cut(s) 268
Csp6I GTAC 3 cut(s) 437, 1043, 1421
CspCI CAANNNNNGTGG 2 cut(s) 1559, 1594
CviAII CATG 7 cut(s) 63, 447, 506, 623, 784, 1126, 1526
CviQI GTAC 3 cut(s) 437, 1043, 1421
DdeI CTNAG 3 cut(s) 617, 726, 1431
DinI GGCGCC 1 cut(s) 1327
DpnI GATC 4 cut(s) 57, 333, 484, 534
DpnII GATC 4 cut(s) 55, 331, 482, 532
Eam1104I CTCTTC 3 cut(s) 160, 301, 1365
EarI CTCTTC 3 cut(s) 160, 301, 1365
EciI GGCGGA 3 cut(s) 98, 165, 277
Eco24I GRGCYC 1 cut(s) 270
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 2 cut(s) 1024, 1534
Eco57I CTGAAG 1 cut(s) 944
Eco88I CYCGRG 2 cut(s) 257, 515
EcoRII CCWGG 2 cut(s) 669, 1321
EcoRV GATATC 1 cut(s) 958
EcoT38I GRGCYC 1 cut(s) 270
EgeI GGCGCC 1 cut(s) 1327
EheI GGCGCC 1 cut(s) 1327
Esp3I CGTCTC 1 cut(s) 157
FaeI CATG 7 cut(s) 66, 450, 509, 626, 787, 1129, 1529
FalI AAGNNNNNCTT 2 cut(s) 809, 841
FaqI GGGAC 3 cut(s) 197, 620, 1520
FatI CATG 7 cut(s) 62, 446, 505, 622, 783, 1125, 1525
FauI CCCGC 3 cut(s) 170, 299, 1408
FauNDI CATATG 1 cut(s) 930
FokI GGATG 3 cut(s) 285, 356, 1241
FriOI GRGCYC 1 cut(s) 270
FspBI CTAG 5 cut(s) 305, 900, 1164, 1290, 1539
GlaI GCGC 2 cut(s) 324, 1327
GsaI CCCAGC 1 cut(s) 313
HaeII RGCGCY 2 cut(s) 326, 1329
HaeIII GGCC 2 cut(s) 568, 1243
HapII CCGG 2 cut(s) 179, 320
HgaI GACGC 1 cut(s) 268
HhaI GCGC 2 cut(s) 325, 1328
Hin1I GRCGYC 1 cut(s) 1326
Hin1II CATG 7 cut(s) 66, 450, 509, 626, 787, 1129, 1529
Hin6I GCGC 2 cut(s) 323, 1326
HinP1I GCGC 2 cut(s) 323, 1326
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 5 cut(s) 608, 893, 1472, 1517, 1567
HpaII CCGG 2 cut(s) 179, 320
Hpy166II GTNNAC 4 cut(s) 1043, 1045, 1377, 1388
Hpy188I TCNGA 9 cut(s) 272, 532, 727, 924, 1028, 1132, 1152, 1471, 1486
Hpy188III TCNNGA 7 cut(s) 259, 515, 517, 540, 900, 1158, 1203
Hpy8I GTNNAC 4 cut(s) 1043, 1045, 1377, 1388
Hpy99I CGWCG 2 cut(s) 117, 284
HpyAV CCTTC 6 cut(s) 69, 109, 263, 453, 579, 870
HpyCH4III ACNGT 4 cut(s) 211, 403, 415, 1040
HpyCH4IV ACGT 1 cut(s) 427
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 17, 184, 190, 242, 245, 1193, 1306
HpyF3I CTNAG 3 cut(s) 617, 726, 1431
HpySE526I ACGT 1 cut(s) 427
Hsp92I GRCGYC 1 cut(s) 1326
Hsp92II CATG 7 cut(s) 66, 450, 509, 626, 787, 1129, 1529
HspAI GCGC 2 cut(s) 323, 1326
KasI GGCGCC 1 cut(s) 1325
KroI GCCGGC 1 cut(s) 319
KroNI GCCGGC 1 cut(s) 321
Kzo9I GATC 4 cut(s) 55, 331, 482, 532
LguI GCTCTTC 1 cut(s) 301
LmnI GCTCC 6 cut(s) 25, 181, 769, 835, 1095, 1459
Lsp1109I GCAGC 8 cut(s) 15, 177, 263, 406, 571, 748, 767, 1575
LweI GCATC 3 cut(s) 48, 263, 976
MaeI CTAG 5 cut(s) 305, 900, 1164, 1290, 1539
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 2 cut(s) 33, 692
MalI GATC 4 cut(s) 57, 333, 484, 534
MbiI CCGCTC 1 cut(s) 294
MboI GATC 4 cut(s) 55, 331, 482, 532
MboII GAAGA 7 cut(s) 147, 288, 564, 677, 888, 1100, 1382
MfeI CAATTG 1 cut(s) 1493
MflI RGATCY 2 cut(s) 55, 532
MhlI GDGCHC 3 cut(s) 270, 408, 1297
Mly113I GGCGCC 1 cut(s) 1326
MlyI GAGTC 2 cut(s) 602, 1466
MmeI TCCRAC 2 cut(s) 1048, 1051
MroNI GCCGGC 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 880
MseI TTAA 4 cut(s) 90, 453, 630, 1305
MslI CAYNNNNRTG 1 cut(s) 772
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 2 cut(s) 179, 320
MspR9I CCNGG 2 cut(s) 671, 1323
MunI CAATTG 1 cut(s) 1493
Mva1269I GAATGC 1 cut(s) 1591
MvaI CCWGG 2 cut(s) 671, 1323
MwoI GCNNNNNNNGC 8 cut(s) 11, 17, 184, 190, 242, 245, 1193, 1306
NaeI GCCGGC 1 cut(s) 321
NarI GGCGCC 1 cut(s) 1326
NdeI CATATG 1 cut(s) 930
NdeII GATC 4 cut(s) 55, 331, 482, 532
NgoMIV GCCGGC 1 cut(s) 319
NlaIII CATG 7 cut(s) 66, 450, 509, 626, 787, 1129, 1529
NlaIV GGNNCC 8 cut(s) 47, 57, 183, 831, 937, 1327, 1461, 1536
NmeAIII GCCGAG 1 cut(s) 1334
NmuCI GTSAC 2 cut(s) 33, 692
NspI RCATGY 1 cut(s) 1129
PaeR7I CTCGAG 1 cut(s) 515
PciSI GCTCTTC 1 cut(s) 301
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 1591
PdiI GCCGGC 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 880
PfeI GAWTC 3 cut(s) 893, 1517, 1567
PleI GAGTC 2 cut(s) 602, 1466
PluTI GGCGCC 1 cut(s) 1329
PpsI GAGTC 2 cut(s) 602, 1466
Ppu21I YACGTR 1 cut(s) 428
Psp6I CCWGG 2 cut(s) 669, 1321
PspFI CCCAGC 1 cut(s) 309
PspGI CCWGG 2 cut(s) 669, 1321
PspN4I GGNNCC 8 cut(s) 47, 57, 183, 831, 937, 1327, 1461, 1536
PspPI GGNCC 3 cut(s) 1024, 1241, 1534
PstNI CAGNNNCTG 2 cut(s) 316, 419
PsuI RGATCY 2 cut(s) 55, 532
RsaI GTAC 3 cut(s) 438, 1044, 1422
RsaNI GTAC 3 cut(s) 437, 1043, 1421
RseI CAYNNNNRTG 1 cut(s) 772
SapI GCTCTTC 1 cut(s) 301
SaqAI TTAA 4 cut(s) 90, 453, 630, 1305
Sau3AI GATC 4 cut(s) 55, 331, 482, 532
Sau96I GGNCC 3 cut(s) 1024, 1241, 1534
SchI GAGTC 2 cut(s) 602, 1466
ScrFI CCNGG 2 cut(s) 671, 1323
SduI GDGCHC 3 cut(s) 270, 408, 1297
SfaNI GCATC 3 cut(s) 48, 263, 976
SfcI CTRYAG 1 cut(s) 326
SfoI GGCGCC 1 cut(s) 1327
Sfr274I CTCGAG 1 cut(s) 515
SinI GGWCC 2 cut(s) 1024, 1534
SlaI CTCGAG 1 cut(s) 515
SmiMI CAYNNNNRTG 1 cut(s) 772
SmlI CTYRAG 5 cut(s) 471, 497, 515, 538, 547
SmoI CTYRAG 5 cut(s) 471, 497, 515, 538, 547
SpeI ACTAGT 1 cut(s) 1163
SspDI GGCGCC 1 cut(s) 1325
SspMI CTAG 5 cut(s) 305, 900, 1164, 1290, 1539
StyD4I CCNGG 2 cut(s) 669, 1321
TaaI ACNGT 4 cut(s) 211, 403, 415, 1040
TaiI ACGT 1 cut(s) 430
TaqI TCGA 3 cut(s) 516, 1204, 1219
TatI WGTACW 1 cut(s) 1042
TauI GCSGC 6 cut(s) 187, 190, 239, 248, 365, 584
TfiI GAWTC 3 cut(s) 893, 1517, 1567
Tru1I TTAA 4 cut(s) 90, 453, 630, 1305
Tru9I TTAA 4 cut(s) 90, 453, 630, 1305
TscAI CASTG 4 cut(s) 1045, 1105, 1268, 1302
TseFI GTSAC 2 cut(s) 33, 692
TseI GCWGC 9 cut(s) 11, 28, 190, 276, 419, 584, 761, 780, 1588
Tsp45I GTSAC 2 cut(s) 33, 692
TspDTI ATGAA 2 cut(s) 51, 435
TspGWI ACGGA 1 cut(s) 356
TspRI CASTG 4 cut(s) 1045, 1105, 1268, 1302
VpaK11BI GGWCC 2 cut(s) 1024, 1534
XapI RAATTY 3 cut(s) 809, 1363, 1573
XbaI TCTAGA 1 cut(s) 899
XceI RCATGY 1 cut(s) 1129
XhoI CTCGAG 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 880
XspI CTAG 5 cut(s) 305, 900, 1164, 1290, 1539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.