Rmu_sc0005811.1_g000005

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005811.1
OG0004478
Physical Location & Seq
Forward (+)
18015 .. 22129
4115 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1518 bp
atgcttttgtgtttgagcttcactgatggcgttgatgttaatccattagctgagtggtgcgactacaaccactgtccttggggatctgcatctgtgttaaatttgaacttgtctcatcctctgcttgccaagtcaattcaagctttcaatcagttgaggatcagaattgggggttccttgcaagatcaagtattgtatggtgtaggagatttgaaatatccatgccatcctttcagaaagcagaagggtgggttgtttggattttctacaggatgtttacctatgagtagatgggatgagcttaaccattttttcagcaagacgaggccccttgtcacatttagtttgaatgcactgtcagggaggcaccacaaaggcgcggatgtttgggttggagattgggactctagcaatgcctatgattttattaattatacagtttcgaagggctaccacataaattcatgggagttgggtaatgagctctgtggtaatggtgttggagctagagttggagctgaacagtatggaaaagacatgatgaagcttaaacaaattgtcaaccagttgtacaatgactcccacataaagcctgcaattgtaggacctggaggattctatgatcaaaagtggtttgccacgcttcttcaggtttctggttcaggcgtagtcaatgttgtcactcagcatctttacaatctgggtccaggtgttgatcccaagttgatgcaaaagatattggatccgttttacttggaccatatatctggtacattcgataatcttgcagcaacagtaagacagcatggaccttgggcttctatatgggttggagagtctggaggagcctataacagcggcggtcataatgtatctgacacatatgtgaacagcttttggtatttggatcagcttggaatgtctgccaagtacaatactgcagtatattgcaggcagtccctagttggtgggcactacggtcttctcaacagaacctcatttgttcccaaccctgattactacagtgcacttctgtggcatcgacttatgggacaaagtgttctcgctgttgaccgtaacggggcaccgcacttgcgcgtttatgctcattgttcaaaaggaagcgccggtataaccctactcctcatcaatttaagcaatcagactgatttcactgtcgatgttaaaaatgttctggagttcgatacagctgctcgatcaagaaacactgacaaaggaaatcatttcagtcatggtcttaagagaacactttcatgggttggtctcaaagcatcagatggaccgttacatagaaaagaataccatctgactccaaaagacgggaaccttagaagcaaaatcatggtcctcaatggagttccattggaaattaccaaggacggaaacatcccggaattagaacctgttcttgttcctacaaattctcctctatcgatcacctctttgtccctcaagtttgttgtgctgcctcactttgaggctccagcttgtggatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko05205 map00531 map01100 map05205
Pfam Domains
Protein Families
Glyco_hydro_79n

Protein Analysis

505

Amino Acids

55.78

Weight (kDa)

8.25

Isoelectric Point (pI)

30.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 17 - 306 8.9e-140 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 366, 1084
AccB7I CCANNNNNTGG 1 cut(s) 1511
AccII CGCG 2 cut(s) 380, 1098
AciI CCGC 4 cut(s) 380, 858, 861, 1088
AclWI GGATC 6 cut(s) 91, 167, 710, 737, 750, 915
AcsI RAATTY 3 cut(s) 100, 460, 1441
AcuI CTGAAG 1 cut(s) 632
AfaI GTAC 3 cut(s) 572, 772, 932
AfiI CCNNNNNNNGG 3 cut(s) 1081, 1340, 1511
AflII CTTAAG 1 cut(s) 1259
AgsI TTSAA 6 cut(s) 106, 140, 148, 214, 349, 1116
AjnI CCWGG 2 cut(s) 607, 706
AleI CACNNNNGTG 2 cut(s) 884, 1033
AloI GAACNNNNNNTCC 2 cut(s) 1044, 1076
Alw21I GWGCWC 2 cut(s) 486, 1030
Alw26I GTCTC 2 cut(s) 117, 1289
Alw44I GTGCAC 1 cut(s) 1026
AlwI GGATC 6 cut(s) 91, 167, 710, 737, 750, 915
AoxI GGCC 1 cut(s) 326
ApaLI GTGCAC 1 cut(s) 1026
ApeKI GCWGC 3 cut(s) 788, 1211, 1486
ApoI RAATTY 3 cut(s) 100, 460, 1441
ArsI GACNNNNNNTTYG 2 cut(s) 1350, 1382
AseI ATTAAT 1 cut(s) 429
Asp700I GAANNNNTTC 2 cut(s) 1270, 1425
AspLEI GCGC 3 cut(s) 380, 1098, 1127
AspS9I GGNCC 7 cut(s) 327, 605, 704, 757, 809, 1301, 1366
AsuC2I CCSGG 1 cut(s) 1412
AsuHPI GGTGA 1 cut(s) 1450
AsuII TTCGAA 1 cut(s) 443
AvaII GGWCC 6 cut(s) 605, 704, 757, 809, 1301, 1366
BaeGI GKGCMC 3 cut(s) 975, 1030, 1087
BamHI GGATCC 1 cut(s) 742
BanI GGYRCC 2 cut(s) 366, 1084
BanII GRGCYC 1 cut(s) 486
BbsI GAAGAC 1 cut(s) 974
Bbv12I GWGCWC 2 cut(s) 486, 1030
BbvI GCAGC 3 cut(s) 800, 1198, 1473
BccI CCATC 5 cut(s) 20, 234, 285, 1292, 1332
BcgI CGANNNNNNTGC 4 cut(s) 767, 801, 1193, 1227
BciT130I CCWGG 2 cut(s) 609, 708
BclI TGATCA 1 cut(s) 622
BcnI CCSGG 1 cut(s) 1412
BcoDI GTCTC 2 cut(s) 117, 1289
BfaI CTAG 3 cut(s) 408, 507, 962
BfmI CTRYAG 3 cut(s) 267, 939, 1021
BfoI RGCGCY 1 cut(s) 1128
BfrI CTTAAG 1 cut(s) 1259
BisI GCNGC 4 cut(s) 789, 859, 1212, 1487
BlsI GCNGC 4 cut(s) 790, 860, 1213, 1488
Bme1390I CCNGG 3 cut(s) 609, 708, 1412
Bme18I GGWCC 6 cut(s) 605, 704, 757, 809, 1301, 1366
BmgT120I GGNCC 7 cut(s) 327, 605, 704, 757, 809, 1301, 1366
BmiI GGNNCC 9 cut(s) 175, 329, 368, 705, 744, 847, 1086, 1346, 1503
BmrFI CCNGG 3 cut(s) 609, 708, 1412
BmsI GCATC 5 cut(s) 98, 697, 717, 1048, 1301
BpiI GAAGAC 1 cut(s) 974
BpmI CTGGAG 4 cut(s) 630, 861, 1217, 1488
Bpu14I TTCGAA 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 1457
BpuMI CCSGG 1 cut(s) 1412
Bsa29I ATCGAT 1 cut(s) 1454
BsaBI GATNNNNATC 2 cut(s) 39, 88
BsaI GGTCTC 1 cut(s) 1289
BsaJI CCNNGG 3 cut(s) 77, 812, 1395
BsaXI ACNNNNNCTCC 6 cut(s) 495, 525, 563, 593, 1429, 1459
Bsc4I CCNNNNNNNGG 3 cut(s) 1081, 1340, 1511
Bse118I RCCGGY 1 cut(s) 1127
Bse1I ACTGG 1 cut(s) 565
Bse3DI GCAATG 1 cut(s) 418
Bse8I GATNNNNATC 2 cut(s) 39, 88
BseBI CCWGG 2 cut(s) 609, 708
BseCI ATCGAT 1 cut(s) 1454
BseDI CCNNGG 3 cut(s) 77, 812, 1395
BseGI GGATG 6 cut(s) 115, 226, 278, 301, 388, 1407
BseJI GATNNNNATC 2 cut(s) 39, 88
BseLI CCNNNNNNNGG 3 cut(s) 1081, 1340, 1511
BseMI GCAATG 1 cut(s) 418
BseMII CTCAG 2 cut(s) 42, 698
BseNI ACTGG 1 cut(s) 565
BseRI GAGGAG 3 cut(s) 858, 1133, 1437
BseSI GKGCMC 3 cut(s) 975, 1030, 1087
BseXI GCAGC 3 cut(s) 800, 1198, 1473
Bsh1236I CGCG 2 cut(s) 380, 1098
BshFI GGCC 1 cut(s) 328
BshNI GGYRCC 2 cut(s) 366, 1084
BshVI ATCGAT 1 cut(s) 1454
BsiHKAI GWGCWC 2 cut(s) 486, 1030
BsiSI CCGG 2 cut(s) 1128, 1412
BslFI GGGAC 4 cut(s) 416, 943, 1065, 1453
BslI CCNNNNNNNGG 3 cut(s) 1081, 1340, 1511
BsmAI GTCTC 2 cut(s) 117, 1289
BsmFI GGGAC 4 cut(s) 416, 943, 1065, 1453
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 1 cut(s) 328
Bso31I GGTCTC 1 cut(s) 1289
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 4 cut(s) 486, 975, 1030, 1087
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 9 cut(s) 83, 159, 184, 622, 715, 742, 907, 1217, 1455
BspACI CCGC 4 cut(s) 380, 858, 861, 1088
BspANI GGCC 1 cut(s) 328
BspCNI CTCAG 2 cut(s) 43, 697
BspDI ATCGAT 1 cut(s) 1454
BspFNI CGCG 2 cut(s) 380, 1098
BspLI GGNNCC 9 cut(s) 175, 329, 368, 705, 744, 847, 1086, 1346, 1503
BspMAI CTGCAG 1 cut(s) 943
BspPI GGATC 6 cut(s) 91, 167, 710, 737, 750, 915
BspT104I TTCGAA 1 cut(s) 443
BspT107I GGYRCC 2 cut(s) 366, 1084
BspTI CTTAAG 1 cut(s) 1259
BspTNI GGTCTC 1 cut(s) 1289
BsrDI GCAATG 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 1127
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 1 cut(s) 565
BssAI RCCGGY 1 cut(s) 1127
BssECI CCNNGG 3 cut(s) 77, 812, 1395
BssMI GATC 9 cut(s) 83, 159, 184, 622, 715, 742, 907, 1217, 1455
BssT1I CCWWGG 3 cut(s) 77, 812, 1395
Bst2UI CCWGG 2 cut(s) 609, 708
BstAFI CTTAAG 1 cut(s) 1259
BstAUI TGTACA 1 cut(s) 570
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 3 cut(s) 126, 594, 953
BstDEI CTNAG 3 cut(s) 51, 684, 1349
BstF5I GGATG 6 cut(s) 115, 226, 278, 301, 388, 1407
BstFNI CGCG 2 cut(s) 380, 1098
BstH2I RGCGCY 1 cut(s) 1128
BstHHI GCGC 3 cut(s) 380, 1098, 1127
BstKTI GATC 9 cut(s) 86, 162, 187, 625, 718, 745, 910, 1220, 1458
BstMAI GTCTC 2 cut(s) 117, 1289
BstMBI GATC 9 cut(s) 83, 159, 184, 622, 715, 742, 907, 1217, 1455
BstNI CCWGG 2 cut(s) 609, 708
BstSCI CCNGG 3 cut(s) 607, 706, 1410
BstSFI CTRYAG 3 cut(s) 267, 939, 1021
BstSLI GKGCMC 3 cut(s) 975, 1030, 1087
BstUI CGCG 2 cut(s) 380, 1098
BstV1I GCAGC 3 cut(s) 800, 1198, 1473
BstV2I GAAGAC 1 cut(s) 974
BstX2I RGATCY 2 cut(s) 83, 742
BstXI CCANNNNNNTGG 1 cut(s) 767
BstYI RGATCY 2 cut(s) 83, 742
Bsu15I ATCGAT 1 cut(s) 1454
BsuRI GGCC 1 cut(s) 328
BsuTUI ATCGAT 1 cut(s) 1454
BtsCI GGATG 6 cut(s) 115, 226, 278, 301, 388, 1407
BtsIMutI CAGTG 6 cut(s) 21, 70, 353, 1030, 1173, 1227
Cac8I GCNNGC 3 cut(s) 126, 594, 953
CfoI GCGC 3 cut(s) 380, 1098, 1127
Cfr10I RCCGGY 1 cut(s) 1127
Cfr13I GGNCC 7 cut(s) 327, 605, 704, 757, 809, 1301, 1366
ClaI ATCGAT 1 cut(s) 1454
Csp6I GTAC 3 cut(s) 571, 771, 931
CspCI CAANNNNNGTGG 2 cut(s) 59, 94
CviAII CATG 7 cut(s) 222, 465, 538, 806, 1253, 1275, 1363
CviQI GTAC 3 cut(s) 571, 771, 931
DdeI CTNAG 3 cut(s) 51, 684, 1349
DpnI GATC 9 cut(s) 85, 161, 186, 624, 717, 744, 909, 1219, 1457
DpnII GATC 9 cut(s) 83, 159, 184, 622, 715, 742, 907, 1217, 1455
Ecl136II GAGCTC 1 cut(s) 484
Eco130I CCWWGG 3 cut(s) 77, 812, 1395
Eco24I GRGCYC 1 cut(s) 486
Eco31I GGTCTC 1 cut(s) 1289
Eco47I GGWCC 6 cut(s) 605, 704, 757, 809, 1301, 1366
Eco53kI GAGCTC 1 cut(s) 484
Eco57I CTGAAG 1 cut(s) 632
EcoICRI GAGCTC 1 cut(s) 484
EcoO109I RGGNCCY 2 cut(s) 327, 605
EcoRII CCWGG 2 cut(s) 607, 706
EcoT14I CCWWGG 3 cut(s) 77, 812, 1395
EcoT38I GRGCYC 1 cut(s) 486
ErhI CCWWGG 3 cut(s) 77, 812, 1395
FaeI CATG 7 cut(s) 225, 468, 541, 809, 1256, 1278, 1366
FaqI GGGAC 4 cut(s) 416, 943, 1065, 1453
FatI CATG 7 cut(s) 221, 464, 537, 805, 1252, 1274, 1362
FauNDI CATATG 1 cut(s) 883
FbaI TGATCA 1 cut(s) 622
Fnu4HI GCNGC 4 cut(s) 789, 859, 1212, 1487
FokI GGATG 6 cut(s) 102, 213, 285, 308, 395, 1394
FriOI GRGCYC 1 cut(s) 486
Fsp4HI GCNGC 4 cut(s) 789, 859, 1212, 1487
FspBI CTAG 3 cut(s) 408, 507, 962
GlaI GCGC 3 cut(s) 379, 1097, 1126
GluI GCNGC 4 cut(s) 789, 859, 1212, 1487
GsuI CTGGAG 4 cut(s) 630, 861, 1217, 1488
HaeII RGCGCY 1 cut(s) 1128
HaeIII GGCC 1 cut(s) 328
HapII CCGG 2 cut(s) 1128, 1412
HhaI GCGC 3 cut(s) 380, 1098, 1127
Hin1II CATG 7 cut(s) 225, 468, 541, 809, 1256, 1278, 1366
Hin6I GCGC 3 cut(s) 378, 1096, 1125
HinP1I GCGC 3 cut(s) 378, 1096, 1125
HincII GTYRAC 2 cut(s) 562, 1072
HindII GTYRAC 2 cut(s) 562, 1072
HindIII AAGCTT 2 cut(s) 141, 545
HinfI GANTC 5 cut(s) 404, 578, 615, 836, 1330
HpaII CCGG 2 cut(s) 1128, 1412
HphI GGTGA 1 cut(s) 1450
Hpy166II GTNNAC 5 cut(s) 278, 562, 889, 1028, 1072
Hpy188I TCNGA 6 cut(s) 164, 236, 877, 1164, 1297, 1329
Hpy188III TCNNGA 3 cut(s) 840, 1196, 1221
Hpy8I GTNNAC 5 cut(s) 278, 562, 889, 1028, 1072
HpyAV CCTTC 2 cut(s) 238, 439
HpyCH4V TGCA 9 cut(s) 89, 181, 353, 596, 730, 788, 941, 951, 1028
HpyF3I CTNAG 3 cut(s) 51, 684, 1349
Hsp92II CATG 7 cut(s) 225, 468, 541, 809, 1256, 1278, 1366
HspAI GCGC 3 cut(s) 378, 1096, 1125
Ksp22I TGATCA 1 cut(s) 622
Kzo9I GATC 9 cut(s) 83, 159, 184, 622, 715, 742, 907, 1217, 1455
LmnI GCTCC 4 cut(s) 503, 515, 845, 1507
Lsp1109I GCAGC 3 cut(s) 800, 1198, 1473
LweI GCATC 5 cut(s) 98, 697, 717, 1048, 1301
MaeI CTAG 3 cut(s) 408, 507, 962
MaeIII GTNAC 4 cut(s) 334, 679, 1076, 1305
MalI GATC 9 cut(s) 85, 161, 186, 624, 717, 744, 909, 1219, 1457
MboI GATC 9 cut(s) 83, 159, 184, 622, 715, 742, 907, 1217, 1455
MboII GAAGA 2 cut(s) 638, 974
MfeI CAATTG 1 cut(s) 597
MflI RGATCY 2 cut(s) 83, 742
MhlI GDGCHC 4 cut(s) 486, 975, 1030, 1087
MlyI GAGTC 4 cut(s) 398, 572, 845, 1324
MmeI TCCRAC 4 cut(s) 373, 481, 493, 811
MroXI GAANNNNTTC 2 cut(s) 1270, 1425
MseI TTAA 8 cut(s) 39, 98, 303, 429, 549, 1154, 1185, 1260
MslI CAYNNNNRTG 3 cut(s) 884, 1033, 1273
MspA1I CMGCKG 2 cut(s) 858, 1211
MspCI CTTAAG 1 cut(s) 1259
MspI CCGG 2 cut(s) 1128, 1412
MspR9I CCNGG 3 cut(s) 609, 708, 1412
MunI CAATTG 1 cut(s) 597
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 2 cut(s) 609, 708
MvnI CGCG 2 cut(s) 380, 1098
NciI CCSGG 1 cut(s) 1412
NdeI CATATG 1 cut(s) 883
NdeII GATC 9 cut(s) 83, 159, 184, 622, 715, 742, 907, 1217, 1455
NlaIII CATG 7 cut(s) 225, 468, 541, 809, 1256, 1278, 1366
NlaIV GGNNCC 9 cut(s) 175, 329, 368, 705, 744, 847, 1086, 1346, 1503
NmuCI GTSAC 2 cut(s) 334, 679
NspV TTCGAA 1 cut(s) 443
OliI CACNNNNGTG 2 cut(s) 884, 1033
PctI GAATGC 1 cut(s) 355
PdmI GAANNNNTTC 2 cut(s) 1270, 1425
PfeI GAWTC 1 cut(s) 615
PflMI CCANNNNNTGG 1 cut(s) 1511
PfoI TCCNGGA 1 cut(s) 1410
PkrI GCNGC 4 cut(s) 790, 860, 1213, 1488
PleI GAGTC 4 cut(s) 398, 572, 844, 1324
PpsI GAGTC 4 cut(s) 398, 572, 844, 1324
PpuMI RGGWCCY 1 cut(s) 605
PshBI ATTAAT 1 cut(s) 429
Psp124BI GAGCTC 1 cut(s) 486
Psp5II RGGWCCY 1 cut(s) 605
Psp6I CCWGG 2 cut(s) 607, 706
PspGI CCWGG 2 cut(s) 607, 706
PspN4I GGNNCC 9 cut(s) 175, 329, 368, 705, 744, 847, 1086, 1346, 1503
PspPI GGNCC 7 cut(s) 327, 605, 704, 757, 809, 1301, 1366
PspPPI RGGWCCY 1 cut(s) 605
PstI CTGCAG 1 cut(s) 943
PsuI RGATCY 2 cut(s) 83, 742
PvuII CAGCTG 1 cut(s) 1211
RsaI GTAC 3 cut(s) 572, 772, 932
RsaNI GTAC 3 cut(s) 571, 771, 931
RseI CAYNNNNRTG 3 cut(s) 884, 1033, 1273
SacI GAGCTC 1 cut(s) 486
SaqAI TTAA 8 cut(s) 39, 98, 303, 429, 549, 1154, 1185, 1260
SatI GCNGC 4 cut(s) 789, 859, 1212, 1487
Sau3AI GATC 9 cut(s) 83, 159, 184, 622, 715, 742, 907, 1217, 1455
Sau96I GGNCC 7 cut(s) 327, 605, 704, 757, 809, 1301, 1366
SchI GAGTC 4 cut(s) 398, 572, 845, 1324
ScrFI CCNGG 3 cut(s) 609, 708, 1412
SduI GDGCHC 4 cut(s) 486, 975, 1030, 1087
SfaNI GCATC 5 cut(s) 98, 697, 717, 1048, 1301
SfcI CTRYAG 3 cut(s) 267, 939, 1021
SfuI TTCGAA 1 cut(s) 443
SinI GGWCC 6 cut(s) 605, 704, 757, 809, 1301, 1366
SmiMI CAYNNNNRTG 3 cut(s) 884, 1033, 1273
SmlI CTYRAG 2 cut(s) 1259, 1472
SmoI CTYRAG 2 cut(s) 1259, 1472
SsiI CCGC 4 cut(s) 380, 858, 861, 1088
SspMI CTAG 3 cut(s) 408, 507, 962
SstI GAGCTC 1 cut(s) 486
StyD4I CCNGG 3 cut(s) 607, 706, 1410
StyI CCWWGG 3 cut(s) 77, 812, 1395
TaqI TCGA 7 cut(s) 443, 777, 1042, 1179, 1203, 1216, 1454
TatI WGTACW 2 cut(s) 570, 930
TauI GCSGC 1 cut(s) 861
TfiI GAWTC 1 cut(s) 615
Tru1I TTAA 8 cut(s) 39, 98, 303, 429, 549, 1154, 1185, 1260
Tru9I TTAA 8 cut(s) 39, 98, 303, 429, 549, 1154, 1185, 1260
TscAI CASTG 6 cut(s) 28, 77, 360, 1030, 1180, 1234
TseFI GTSAC 2 cut(s) 334, 679
TseI GCWGC 3 cut(s) 788, 1211, 1486
Tsp45I GTSAC 2 cut(s) 334, 679
TspDTI ATGAA 3 cut(s) 453, 557, 1263
TspGWI ACGGA 2 cut(s) 735, 1416
TspRI CASTG 6 cut(s) 28, 77, 360, 1030, 1180, 1234
Van91I CCANNNNNTGG 1 cut(s) 1511
Vha464I CTTAAG 1 cut(s) 1259
VneI GTGCAC 1 cut(s) 1026
VpaK11BI GGWCC 6 cut(s) 605, 704, 757, 809, 1301, 1366
VspI ATTAAT 1 cut(s) 429
XapI RAATTY 3 cut(s) 100, 460, 1441
XcmI CCANNNNNNNNNTGG 1 cut(s) 51
XmnI GAANNNNTTC 2 cut(s) 1270, 1425
XspI CTAG 3 cut(s) 408, 507, 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.