Rmu_sc0006733.1_g000002
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006733.1
OG0012697
Physical Location & Seq
Reverse (-)
6500 .. 11654
5155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006733.1_g000002.1.cds

Sequence Viewer

Length: 813 bp
atggtggttactgattgggtctggtttgtcggtcttgatgtgtgctccttaactctggtattagaccgtgcctcgaaatctctaccttcactaagaagcataccagaactgtacaaagagaaagagcagaatttgaaggctttctcattccaagagctcaggaattcaaccaatggcttcagcaggttgcaaaagcttggtgaaggaggctttcggagtgtgtataaaggaataatcatgcccaaaaatggccaggccttcgtctcctcttctaatggtggtagtgagtgttgggatagtggcggtggactcggtgaaagcagccttggctggtcttatgcatatgaatggtctttgcctccctcatttcttcattctctttctgctgatggtgggattagggtgacgatgatggattttctagttttgaatccccgagacgacctctcttcctcctctttgggtgggtttgagctgactgcattgggatctactcttgagaacggaggtgatgacggaggagagtcaatagccacctatagatctactcttgagaacggaggtgatgacggaggagagtcaatagccacctccagcgctgtgagcggtgatccaaaccatggcggcggtgcttactgtttggaggagccggtttgtgctttgttttgttcgcaggtggtcgatgctatcattggttgtcggctgaagtggttggtaggtagtacggtggaaatcgaggcagatgctttggctgccggcagagggaagtttggggcgattgacagtgacctagattggcagaatggcagctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

270

Amino Acids

28.58

Weight (kDa)

4.43

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 664
Acc36I ACCTGC 2 cut(s) 174, 664
AccB7I CCANNNNNTGG 1 cut(s) 620
AccBSI CCGCTC 1 cut(s) 606
AciI CCGC 4 cut(s) 303, 606, 624, 627
AclWI GGATC 2 cut(s) 496, 605
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 2 cut(s) 130, 163
AcuI CTGAAG 2 cut(s) 163, 725
AfaI GTAC 2 cut(s) 113, 724
AfeI AGCGCT 1 cut(s) 598
AfiI CCNNNNNNNGG 3 cut(s) 248, 620, 762
AgsI TTSAA 3 cut(s) 136, 168, 430
AjnI CCWGG 1 cut(s) 252
AjuI GAANNNNNNNTTGG 2 cut(s) 309, 341
AluBI AGCT 4 cut(s) 157, 196, 475, 810
AluI AGCT 4 cut(s) 157, 196, 475, 810
Alw21I GWGCWC 2 cut(s) 47, 159
Alw26I GTCTC 2 cut(s) 268, 432
AlwI GGATC 2 cut(s) 496, 605
Ama87I CYCGRG 1 cut(s) 435
Aor51HI AGCGCT 1 cut(s) 598
AoxI GGCC 2 cut(s) 250, 255
ApeKI GCWGC 3 cut(s) 321, 752, 807
ApoI RAATTY 2 cut(s) 130, 163
Asp700I GAANNNNTTC 1 cut(s) 140
AspLEI GCGC 1 cut(s) 599
AsuHPI GGTGA 6 cut(s) 212, 326, 415, 521, 575, 620
AvaI CYCGRG 1 cut(s) 435
BalI TGGCCA 1 cut(s) 252
BanII GRGCYC 1 cut(s) 159
Bbv12I GWGCWC 2 cut(s) 47, 159
BbvI GCAGC 2 cut(s) 333, 739
BccI CCATC 2 cut(s) 383, 406
BcgI CGANNNNNNTGC 2 cut(s) 725, 759
BciT130I CCWGG 1 cut(s) 254
BcoDI GTCTC 2 cut(s) 268, 432
BfaI CTAG 3 cut(s) 422, 791, 811
BfmI CTRYAG 1 cut(s) 538
BfoI RGCGCY 1 cut(s) 600
BfuAI ACCTGC 2 cut(s) 174, 664
BglII AGATCT 1 cut(s) 542
BisI GCNGC 4 cut(s) 322, 625, 753, 808
BlsI GCNGC 4 cut(s) 323, 626, 754, 809
Bme1390I CCNGG 1 cut(s) 254
BmeT110I CYCGRG 1 cut(s) 435
BmiI GGNNCC 1 cut(s) 648
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 2 cut(s) 673, 733
BplI GAGNNNNNCTC 2 cut(s) 429, 461
BpmI CTGGAG 1 cut(s) 577
Bpu10I CCTNAGC 1 cut(s) 158
BpuEI CTTGAG 2 cut(s) 518, 572
BsaJI CCNNGG 2 cut(s) 325, 619
Bsc4I CCNNNNNNNGG 3 cut(s) 248, 620, 762
Bse118I RCCGGY 2 cut(s) 649, 755
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 2 cut(s) 325, 619
BseLI CCNNNNNNNGG 3 cut(s) 248, 620, 762
BseMII CTCAG 1 cut(s) 172
BseRI GAGGAG 5 cut(s) 256, 445, 534, 588, 659
BseXI GCAGC 2 cut(s) 333, 739
BshFI GGCC 2 cut(s) 252, 257
BsiHKAI GWGCWC 2 cut(s) 47, 159
BsiHKCI CYCGRG 1 cut(s) 435
BsiSI CCGG 2 cut(s) 650, 756
BslI CCNNNNNNNGG 3 cut(s) 248, 620, 762
BsmAI GTCTC 2 cut(s) 268, 432
BsmBI CGTCTC 2 cut(s) 268, 432
BsnI GGCC 2 cut(s) 252, 257
BsoBI CYCGRG 1 cut(s) 435
Bsp1286I GDGCHC 2 cut(s) 47, 159
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 3 cut(s) 488, 542, 610
Bsp19I CCATGG 1 cut(s) 619
BspACI CCGC 4 cut(s) 303, 606, 624, 627
BspANI GGCC 2 cut(s) 252, 257
BspCNI CTCAG 1 cut(s) 171
BspLI GGNNCC 1 cut(s) 648
BspMI ACCTGC 2 cut(s) 174, 664
BspPI GGATC 2 cut(s) 496, 605
BsrBI CCGCTC 1 cut(s) 606
BsrFI RCCGGY 2 cut(s) 649, 755
BsrGI TGTACA 1 cut(s) 111
BssAI RCCGGY 2 cut(s) 649, 755
BssECI CCNNGG 2 cut(s) 325, 619
BssMI GATC 3 cut(s) 488, 542, 610
BssT1I CCWWGG 2 cut(s) 325, 619
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 5 cut(s) 68, 111, 638, 727, 785
Bst6I CTCTTC 2 cut(s) 274, 454
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 757
BstDEI CTNAG 2 cut(s) 92, 158
BstDSI CCRYGG 1 cut(s) 619
BstH2I RGCGCY 1 cut(s) 600
BstHHI GCGC 1 cut(s) 599
BstKTI GATC 3 cut(s) 491, 545, 613
BstMAI GTCTC 2 cut(s) 268, 432
BstMBI GATC 3 cut(s) 488, 542, 610
BstMWI GCNNNNNNNGC 3 cut(s) 327, 603, 752
BstNI CCWGG 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 252
BstSFI CTRYAG 1 cut(s) 538
BstV1I GCAGC 2 cut(s) 333, 739
BstX2I RGATCY 2 cut(s) 488, 542
BstYI RGATCY 2 cut(s) 488, 542
BsuRI GGCC 2 cut(s) 252, 257
BtgI CCRYGG 1 cut(s) 619
BtsIMutI CAGTG 1 cut(s) 790
BveI ACCTGC 2 cut(s) 174, 664
Cac8I GCNNGC 1 cut(s) 757
CfoI GCGC 1 cut(s) 599
Cfr10I RCCGGY 2 cut(s) 649, 755
Csp6I GTAC 2 cut(s) 112, 723
CviAII CATG 2 cut(s) 238, 620
CviQI GTAC 2 cut(s) 112, 723
DdeI CTNAG 2 cut(s) 92, 158
DpnI GATC 3 cut(s) 490, 544, 612
DpnII GATC 3 cut(s) 488, 542, 610
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 2 cut(s) 274, 454
EarI CTCTTC 2 cut(s) 274, 454
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 325, 619
Eco147I AGGCCT 1 cut(s) 257
Eco24I GRGCYC 1 cut(s) 159
Eco47III AGCGCT 1 cut(s) 598
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 2 cut(s) 163, 725
Eco88I CYCGRG 1 cut(s) 435
EcoICRI GAGCTC 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 252
EcoT14I CCWWGG 2 cut(s) 325, 619
EcoT22I ATGCAT 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 2 cut(s) 325, 619
Esp3I CGTCTC 2 cut(s) 268, 432
FaeI CATG 2 cut(s) 241, 623
FaiI YATR 8 cut(s) 101, 225, 239, 339, 343, 345, 540, 621
FatI CATG 2 cut(s) 237, 619
FauNDI CATATG 1 cut(s) 343
Fnu4HI GCNGC 4 cut(s) 322, 625, 753, 808
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 4 cut(s) 322, 625, 753, 808
FspBI CTAG 3 cut(s) 422, 791, 811
GlaI GCGC 1 cut(s) 598
GluI GCNGC 4 cut(s) 322, 625, 753, 808
GsuI CTGGAG 1 cut(s) 577
HaeII RGCGCY 1 cut(s) 600
HaeIII GGCC 2 cut(s) 252, 257
HapII CCGG 2 cut(s) 650, 756
HhaI GCGC 1 cut(s) 599
Hin1II CATG 2 cut(s) 241, 623
Hin6I GCGC 1 cut(s) 597
HinP1I GCGC 1 cut(s) 597
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 4 cut(s) 309, 430, 524, 578
HpaII CCGG 2 cut(s) 650, 756
HphI GGTGA 6 cut(s) 212, 326, 415, 521, 575, 620
Hpy166II GTNNAC 1 cut(s) 308
Hpy188I TCNGA 1 cut(s) 216
Hpy188III TCNNGA 4 cut(s) 35, 160, 497, 551
Hpy8I GTNNAC 1 cut(s) 308
HpyAV CCTTC 4 cut(s) 96, 130, 197, 268
HpyCH4III ACNGT 5 cut(s) 68, 111, 638, 727, 785
HpyCH4V TGCA 3 cut(s) 190, 341, 482
HpyF10VI GCNNNNNNNGC 3 cut(s) 327, 603, 752
HpyF3I CTNAG 2 cut(s) 92, 158
Hsp92II CATG 2 cut(s) 241, 623
HspAI GCGC 1 cut(s) 597
KroI GCCGGC 1 cut(s) 755
KroNI GCCGGC 1 cut(s) 757
Kzo9I GATC 3 cut(s) 488, 542, 610
LmnI GCTCC 2 cut(s) 50, 646
Lsp1109I GCAGC 2 cut(s) 333, 739
LweI GCATC 2 cut(s) 673, 733
MaeI CTAG 3 cut(s) 422, 791, 811
MaeIII GTNAC 3 cut(s) 7, 403, 785
MalI GATC 3 cut(s) 490, 544, 612
MbiI CCGCTC 1 cut(s) 606
MboI GATC 3 cut(s) 488, 542, 610
MboII GAAGA 3 cut(s) 261, 362, 441
MflI RGATCY 2 cut(s) 488, 542
MhlI GDGCHC 2 cut(s) 47, 159
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 2 cut(s) 130, 163
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 3 cut(s) 303, 533, 587
Mox20I TGGCCA 1 cut(s) 252
Mph1103I ATGCAT 1 cut(s) 343
MroNI GCCGGC 1 cut(s) 755
MroXI GAANNNNTTC 1 cut(s) 140
MscI TGGCCA 1 cut(s) 252
MseI TTAA 1 cut(s) 50
MslI CAYNNNNRTG 1 cut(s) 346
Msp20I TGGCCA 1 cut(s) 252
MspI CCGG 2 cut(s) 650, 756
MspR9I CCNGG 1 cut(s) 254
MvaI CCWGG 1 cut(s) 254
MwoI GCNNNNNNNGC 3 cut(s) 327, 603, 752
NaeI GCCGGC 1 cut(s) 757
NcoI CCATGG 1 cut(s) 619
NdeI CATATG 1 cut(s) 343
NdeII GATC 3 cut(s) 488, 542, 610
NgoMIV GCCGGC 1 cut(s) 755
NlaIII CATG 2 cut(s) 241, 623
NlaIV GGNNCC 1 cut(s) 648
NmuCI GTSAC 2 cut(s) 403, 785
NsiI ATGCAT 1 cut(s) 343
PaqCI CACCTGC 1 cut(s) 664
PceI AGGCCT 1 cut(s) 257
PdiI GCCGGC 1 cut(s) 757
PdmI GAANNNNTTC 1 cut(s) 140
PfeI GAWTC 1 cut(s) 430
PflMI CCANNNNNTGG 1 cut(s) 620
PkrI GCNGC 4 cut(s) 323, 626, 754, 809
PleI GAGTC 3 cut(s) 303, 532, 586
PpsI GAGTC 3 cut(s) 303, 532, 586
Psp124BI GAGCTC 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 252
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 1 cut(s) 648
PsuI RGATCY 2 cut(s) 488, 542
RsaI GTAC 2 cut(s) 113, 724
RsaNI GTAC 2 cut(s) 112, 723
RseI CAYNNNNRTG 1 cut(s) 346
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 1 cut(s) 50
SatI GCNGC 4 cut(s) 322, 625, 753, 808
Sau3AI GATC 3 cut(s) 488, 542, 610
SchI GAGTC 3 cut(s) 303, 533, 587
ScrFI CCNGG 1 cut(s) 254
SduI GDGCHC 2 cut(s) 47, 159
SfaNI GCATC 2 cut(s) 673, 733
SfcI CTRYAG 1 cut(s) 538
SmiMI CAYNNNNRTG 1 cut(s) 346
SmlI CTYRAG 2 cut(s) 497, 551
SmoI CTYRAG 2 cut(s) 497, 551
Sse9I AATT 2 cut(s) 130, 163
SseBI AGGCCT 1 cut(s) 257
SsiI CCGC 4 cut(s) 303, 606, 624, 627
SspMI CTAG 3 cut(s) 422, 791, 811
SstI GAGCTC 1 cut(s) 159
StuI AGGCCT 1 cut(s) 257
StyD4I CCNGG 1 cut(s) 252
StyI CCWWGG 2 cut(s) 325, 619
TaaI ACNGT 5 cut(s) 68, 111, 638, 727, 785
TaqI TCGA 3 cut(s) 74, 681, 735
TaqII GACCGA 1 cut(s) 20
TasI AATT 2 cut(s) 130, 163
TatI WGTACW 1 cut(s) 111
TauI GCSGC 1 cut(s) 627
TfiI GAWTC 1 cut(s) 430
Tru1I TTAA 1 cut(s) 50
Tru9I TTAA 1 cut(s) 50
TscAI CASTG 1 cut(s) 790
TseFI GTSAC 2 cut(s) 403, 785
TseI GCWGC 3 cut(s) 321, 752, 807
Tsp45I GTSAC 2 cut(s) 403, 785
TspDTI ATGAA 2 cut(s) 360, 362
TspGWI ACGGA 4 cut(s) 519, 531, 573, 585
TspRI CASTG 1 cut(s) 790
Van91I CCANNNNNTGG 1 cut(s) 620
XapI RAATTY 2 cut(s) 130, 163
XmnI GAANNNNTTC 1 cut(s) 140
XspI CTAG 3 cut(s) 422, 791, 811
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.