Rmu_sc0007137.1_g000007

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007137.1
OG0010779
Physical Location & Seq
Reverse (-)
61949 .. 62757
809 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 726 bp
atgcttagagcattgggcttcggaagtgatgttcacttgtatgtggcatcaggtgaagtgtataaaggtgaagagacattggcaccgctgaaagcacttttcccaaacttccatacaaaggagactatagcaagtaaggaagagttggaaccattttcttccttctcttctcgcatggctgcactagatttcattgtctgtgatgaaagtgatgttttcgtcaccaacaataatggcaacatggctagaatattagctggtcgaaggagatactttgggcataaaccaacaatccgtccaaacactaagaaactgtacaagttgttcattaaccaaaataacatgacatgggaggaatttgcctcccgggttcgaactagtcaaattggatctatgggagagccaaatgagaaaaagccaggcaagggtgagttccatgaaaatccagcagactgcatctgtaacacatctaatacaaagtccaaaaatgtccgaattcctcaaagtgaaatcaatgaaggcagtaacatcagcaaggaagatgagacaagtaaggatagtggttatgcgacagatgggcacatgacggatgacgagcaattttggcctgagatggattatgtggatactggaaacaagtctctgggaaaagcgcctagtcaaagattttcgtatcagggtcttaattatgatctgccagaacttctagagctcttttcagactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

241

Amino Acids

27.18

Weight (kDa)

5.27

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 1 - 91 9.8e-26 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 82
AciI CCGC 1 cut(s) 86
AclWI GGATC 1 cut(s) 397
AcsI RAATTY 2 cut(s) 356, 495
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 2 cut(s) 118, 425
AhlI ACTAGT 1 cut(s) 377
AjnI CCWGG 1 cut(s) 418
AloI GAACNNNNNNTCC 2 cut(s) 15, 47
AluBI AGCT 2 cut(s) 257, 712
AluI AGCT 2 cut(s) 257, 712
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 4 cut(s) 68, 116, 539, 645
AlwI GGATC 1 cut(s) 397
Ama87I CYCGRG 1 cut(s) 366
AoxI GGCC 1 cut(s) 605
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 2 cut(s) 356, 495
AspLEI GCGC 1 cut(s) 655
AsuC2I CCSGG 2 cut(s) 367, 368
AsuHPI GGTGA 4 cut(s) 65, 80, 214, 440
AsuII TTCGAA 1 cut(s) 373
AvaI CYCGRG 1 cut(s) 366
BaeGI GKGCMC 1 cut(s) 582
BanI GGYRCC 1 cut(s) 82
BanII GRGCYC 1 cut(s) 714
Bbv12I GWGCWC 1 cut(s) 714
BbvI GCAGC 1 cut(s) 166
BccI CCATC 2 cut(s) 569, 607
BciT130I CCWGG 1 cut(s) 420
BciVI GTATCC 1 cut(s) 619
BcnI CCSGG 2 cut(s) 367, 368
BcoDI GTCTC 4 cut(s) 68, 116, 539, 645
BcuI ACTAGT 1 cut(s) 377
BfaI CTAG 5 cut(s) 185, 246, 378, 657, 707
BfmI CTRYAG 1 cut(s) 126
BfoI RGCGCY 1 cut(s) 656
BfuI GTATCC 1 cut(s) 619
BisI GCNGC 1 cut(s) 180
BlsI GCNGC 1 cut(s) 181
Bme1390I CCNGG 3 cut(s) 367, 368, 420
BmeT110I CYCGRG 1 cut(s) 366
BmiI GGNNCC 2 cut(s) 84, 150
BmrFI CCNGG 3 cut(s) 367, 368, 420
BmsI GCATC 2 cut(s) 56, 465
Bpu14I TTCGAA 1 cut(s) 373
BpuMI CCSGG 2 cut(s) 367, 368
BsaJI CCNNGG 1 cut(s) 366
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 425
Bse1I ACTGG 1 cut(s) 634
BseBI CCWGG 1 cut(s) 420
BseDI CCNNGG 1 cut(s) 366
BseGI GGATG 1 cut(s) 595
BseLI CCNNNNNNNGG 2 cut(s) 118, 425
BseMII CTCAG 1 cut(s) 600
BseNI ACTGG 1 cut(s) 634
BseSI GKGCMC 1 cut(s) 582
BseXI GCAGC 1 cut(s) 166
BsgI GTGCAG 1 cut(s) 165
BshFI GGCC 1 cut(s) 607
BshNI GGYRCC 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 714
BsiHKCI CYCGRG 1 cut(s) 366
BsiSI CCGG 1 cut(s) 367
BslI CCNNNNNNNGG 2 cut(s) 118, 425
BsmAI GTCTC 4 cut(s) 68, 116, 539, 645
BsnI GGCC 1 cut(s) 607
BsoBI CYCGRG 1 cut(s) 366
Bsp119I TTCGAA 1 cut(s) 373
Bsp1286I GDGCHC 2 cut(s) 582, 714
Bsp1407I TGTACA 1 cut(s) 315
Bsp143I GATC 2 cut(s) 389, 691
BspACI CCGC 1 cut(s) 86
BspANI GGCC 1 cut(s) 607
BspCNI CTCAG 1 cut(s) 601
BspLI GGNNCC 2 cut(s) 84, 150
BspPI GGATC 1 cut(s) 397
BspT104I TTCGAA 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 82
BsrGI TGTACA 1 cut(s) 315
BsrI ACTGG 1 cut(s) 634
BssECI CCNNGG 1 cut(s) 366
BssMI GATC 2 cut(s) 389, 691
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 1 cut(s) 315
Bst6I CTCTTC 3 cut(s) 66, 135, 172
BstAUI TGTACA 1 cut(s) 315
BstBI TTCGAA 1 cut(s) 373
BstDEI CTNAG 3 cut(s) 5, 306, 609
BstF5I GGATG 1 cut(s) 595
BstH2I RGCGCY 1 cut(s) 656
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 2 cut(s) 392, 694
BstMAI GTCTC 4 cut(s) 68, 116, 539, 645
BstMBI GATC 2 cut(s) 389, 691
BstMWI GCNNNNNNNGC 1 cut(s) 604
BstNI CCWGG 1 cut(s) 420
BstSCI CCNGG 3 cut(s) 365, 366, 418
BstSFI CTRYAG 1 cut(s) 126
BstSLI GKGCMC 1 cut(s) 582
BstV1I GCAGC 1 cut(s) 166
BstX2I RGATCY 1 cut(s) 389
BstYI RGATCY 1 cut(s) 389
BsuI GTATCC 1 cut(s) 619
BsuRI GGCC 1 cut(s) 607
BtsCI GGATG 1 cut(s) 595
CfoI GCGC 1 cut(s) 655
Cfr9I CCCGGG 1 cut(s) 366
Csp6I GTAC 1 cut(s) 316
CviAII CATG 6 cut(s) 175, 241, 343, 348, 437, 583
CviJI RGCY 8 cut(s) 18, 179, 245, 257, 403, 418, 607, 712
CviKI_1 RGCY 8 cut(s) 18, 179, 245, 257, 403, 418, 607, 712
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 3 cut(s) 5, 306, 609
DpnI GATC 2 cut(s) 391, 693
DpnII GATC 2 cut(s) 389, 691
Eam1104I CTCTTC 3 cut(s) 66, 135, 172
EarI CTCTTC 3 cut(s) 66, 135, 172
Ecl136II GAGCTC 1 cut(s) 712
Eco24I GRGCYC 1 cut(s) 714
Eco53kI GAGCTC 1 cut(s) 712
Eco88I CYCGRG 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 712
EcoRI GAATTC 1 cut(s) 495
EcoRII CCWGG 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 714
FaeI CATG 6 cut(s) 178, 244, 346, 351, 440, 586
FatI CATG 6 cut(s) 174, 240, 342, 347, 436, 582
Fnu4HI GCNGC 1 cut(s) 180
FokI GGATG 1 cut(s) 602
FriOI GRGCYC 1 cut(s) 714
Fsp4HI GCNGC 1 cut(s) 180
FspBI CTAG 5 cut(s) 185, 246, 378, 657, 707
GlaI GCGC 1 cut(s) 654
GluI GCNGC 1 cut(s) 180
HaeII RGCGCY 1 cut(s) 656
HaeIII GGCC 1 cut(s) 607
HapII CCGG 1 cut(s) 367
HhaI GCGC 1 cut(s) 655
Hin1II CATG 6 cut(s) 178, 244, 346, 351, 440, 586
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HpaII CCGG 1 cut(s) 367
HphI GGTGA 4 cut(s) 65, 80, 214, 440
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 3 cut(s) 23, 494, 721
Hpy188III TCNNGA 1 cut(s) 707
Hpy8I GTNNAC 1 cut(s) 34
HpyAV CCTTC 3 cut(s) 172, 258, 512
HpyCH4III ACNGT 1 cut(s) 315
HpyCH4V TGCA 2 cut(s) 182, 456
HpyF10VI GCNNNNNNNGC 1 cut(s) 604
HpyF3I CTNAG 3 cut(s) 5, 306, 609
Hsp92II CATG 6 cut(s) 178, 244, 346, 351, 440, 586
HspAI GCGC 1 cut(s) 653
Kzo9I GATC 2 cut(s) 389, 691
Lsp1109I GCAGC 1 cut(s) 166
LweI GCATC 2 cut(s) 56, 465
MaeI CTAG 5 cut(s) 185, 246, 378, 657, 707
MaeIII GTNAC 3 cut(s) 220, 461, 524
MalI GATC 2 cut(s) 391, 693
MboI GATC 2 cut(s) 389, 691
MboII GAAGA 5 cut(s) 83, 150, 152, 159, 551
MflI RGATCY 1 cut(s) 389
MhlI GDGCHC 2 cut(s) 582, 714
MluCI AATT 5 cut(s) 356, 384, 495, 599, 685
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 3 cut(s) 346, 373, 510
MseI TTAA 2 cut(s) 330, 684
MslI CAYNNNNRTG 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 88
MspI CCGG 1 cut(s) 367
MspR9I CCNGG 3 cut(s) 367, 368, 420
MvaI CCWGG 1 cut(s) 420
MwoI GCNNNNNNNGC 1 cut(s) 604
NciI CCSGG 2 cut(s) 367, 368
NdeII GATC 2 cut(s) 389, 691
NlaIII CATG 6 cut(s) 178, 244, 346, 351, 440, 586
NlaIV GGNNCC 2 cut(s) 84, 150
NmuCI GTSAC 1 cut(s) 220
NspV TTCGAA 1 cut(s) 373
PkrI GCNGC 1 cut(s) 181
Psp124BI GAGCTC 1 cut(s) 714
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 2 cut(s) 84, 150
PsrI GAACNNNNNNTAC 2 cut(s) 308, 340
PsuI RGATCY 1 cut(s) 389
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
RseI CAYNNNNRTG 1 cut(s) 39
SacI GAGCTC 1 cut(s) 714
SaqAI TTAA 2 cut(s) 330, 684
SatI GCNGC 1 cut(s) 180
Sau3AI GATC 2 cut(s) 389, 691
ScrFI CCNGG 3 cut(s) 367, 368, 420
SduI GDGCHC 2 cut(s) 582, 714
SetI ASST 4 cut(s) 55, 70, 259, 714
SfaNI GCATC 2 cut(s) 56, 465
SfcI CTRYAG 1 cut(s) 126
SfuI TTCGAA 1 cut(s) 373
SmaI CCCGGG 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 39
SpeI ACTAGT 1 cut(s) 377
Sse9I AATT 5 cut(s) 356, 384, 495, 599, 685
SsiI CCGC 1 cut(s) 86
SspI AATATT 1 cut(s) 252
SspMI CTAG 5 cut(s) 185, 246, 378, 657, 707
SstI GAGCTC 1 cut(s) 714
StyD4I CCNGG 3 cut(s) 365, 366, 418
TaaI ACNGT 1 cut(s) 315
TaqI TCGA 2 cut(s) 262, 373
TasI AATT 5 cut(s) 356, 384, 495, 599, 685
TatI WGTACW 1 cut(s) 315
Tru1I TTAA 2 cut(s) 330, 684
Tru9I TTAA 2 cut(s) 330, 684
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 179
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 5 cut(s) 181, 219, 316, 453, 531
TspGWI ACGGA 2 cut(s) 284, 602
TspMI CCCGGG 1 cut(s) 366
XapI RAATTY 2 cut(s) 356, 495
XbaI TCTAGA 1 cut(s) 706
XmaI CCCGGG 1 cut(s) 366
XspI CTAG 5 cut(s) 185, 246, 378, 657, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.