Rmu_sc0007355.1_g000011

Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007355.1
OG0007314
Physical Location & Seq
Reverse (-)
33389 .. 48442
15054 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3174 bp
atgcgaggcctctctgttttcatcagcgacattcggaattgccagaacaaagagcaggagaggctccgtgtcgataaggagctcgggaatgttcgtacgcgcttcaaaaacgaaaagggtttgacaccatatgagaagaagaaatatgtctggaaaatgctttacatttatatgttgggatatgatgtggattttggtcacatggaagcagtctctcttatatcagcgcccaagtatccagaaaagcaggttgggtacattgtgacatcatgtctactcaatgaaaaccacgattttctgagattagcgatcaatactgtacggaatgatattattggtcgtaatgaaactttccaatgtttggcattgactatggttgggaatattggaggcagagaatttgcagagtcattagcacctgatgttcaaaagttgcttatttctagcagctacagaccgcttgtgagaaaaaaagctgcactatgtcttttgcgcttgtatagaaaaaatcctgatgttgtcaatatagatggctgggccgatcgcatggcacaacttttagatgaacgtgatcttggtgttttgacatcatctatgagccttcttgttgcgcttgtatcaaatcaccacgatgcttactggagttgtcttccaaaatgtgttaaaatactagaacggcttgcgaggaaccaggatattccacaagagtacacctactatggcatcccatctccctggctccaggtcaagacaatgagggttctgcagtatttccccactgttgaagatccaaatacaagaagatcattgtttgaggtcttacaacgtatattgatgggtacggatgttgtgaaaaatgttaataagaacaacgcatcacacgctgttctttttgaagccctcgcacttgtcatgcatcttgatgctgagaaggagatgatgtcccaatgtgttgcacttctgggaaaatttattgctgtgcgagaaccaaatattcgatatcttggtttggaaaatatgacccgcatgctaatggtaacggatgtgcaagatatcatcaagagacatcaagcccagattatcacctcattgaaggatccagatgtcagcattcgtaggcgtgctcttgacctgctttatggcatgtgtgatgtttcaaatgcaaaagacatagttgaagaattactgcagtatcttagtacagcagacttcgcaatgcgcgaggaattgtcacttaaggctgccattcttgcagaaaaatttgctcctgatctatcatggtatgtagatgtgattcttcaattgattgataaggcaggagattttgttagtgatgatatatggttccgtgttgtgcaatttgttacgaataatgaagacttacaggtcagagtgtccaatttctgtcaaaagaggtccatagccagagagtatcttgacaaacctgctatacatgagacaatggttaaggtcagtgcatatatcattggagaatttggtcaccttttagccagacggcctgggtgtagtccaaaagaattatttgccgtcatacatgagaagcttcctactgtatccacttctacaatacctattcttctttcaacatatgctaagatctttatgcacactcaacctccagaccaagaactacagaatcagatttgggcaatattcaataaatacgagagttgcatcgatgttgagattcaacaacgagctgctgaataccttgccttaagtaggaggggtgaagctcttgttgatatattggctgaaatgcctaaattccccgagcggcagtctgcattgatcaaaaaggctgaagatactgaagttgataccgcagaacaaagtgctatcaagttgcgtgcccagcagcaaacatcaaatgctttggttgtgaccgaccaacgtcccgctaatggtaccccacctgccaaccaccagcttggtcttgtgaagatacccaacatgagcagtaatgcggatcacaattcaaccgatcaagggttgtctcaggaaaatggtactttgaataaagttgatcctcaaactccttcaccggatctccttggtgacctcttgggtccactggcaattgaaggccctccgggtactgccgttcaatcacatcaaactgaaatccctggatctggtggtgacccaaatgcagtggatgctacggccatagttcctgttggcgaagagccaaattctgttcagcctataggaaatattgcagaaagatttcatgccttgtgcttgaaagatagtggcgttctgtatgaagatcctaatattcagatcggaattaaagcagagtggcgcttacatcagggatgtcttgttcttttcttgggaaacaaaaatacttcaccacttgtatcagttcaggctgtaataatgcctccctcccactttaaaatggagctctcactagtgccagagactattcctcctagggcacaggtacagtgcccccttgaagttgtgaatctccgcccaagtagggatgtagcagttcttgacttctcttacaagttcggtcacaatatggtcaatgtcaagcttcgtctcccagctgtcttgaataaatttcttcaacctataccagtatctgctgaagagttctttcctcagtggagatcactcacaggaccacctctgaagcttcaagaagtggttagaggcgtcaaaccactgccacttgcagaaatggcaaatctaattaacagcttccggtttatggtttgtccagggcttgaccctaatccaaataatcttgttgcaagcacaaccttttactcagaaagtacacgggcgatgctttgcttggctagaatagaaacagatccagcagacagaacccagttgcgtatgacggttgcttcaggggatcctacgttaacatttgagttgaaggagttcatcaaggaacaaatagttaacatccctgtagctcctcgtgcacctggaccagtgccaccagcacctccagtagctcaaccgaccagtccagctgcagcattaacggatcctggtgctctgctggctgggtctcgcgcgcaagttaaaaatgtggataacaaaaatcctgatgcaagggctgtcggaatcaaggatgagctaacttgtgtgaacggcttggtgttgtggatctgcacaggttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 Alpha_adaptin_C

Protein Analysis

1057

Amino Acids

117.92

Weight (kDa)

6.14

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 23 - 583 3.5e-113 Adaptin N terminal region
Cnd1 PF12717 120 - 258 1.1e-06 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 759 - 855 2e-13 Adaptin C-terminal domain
Alpha_adaptin_C PF02296 867 - 976 2.5e-15 Alpha adaptin AP2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1945
AasI GACNNNNNNGTC 1 cut(s) 1385
Acc36I ACCTGC 4 cut(s) 238, 1140, 1453, 1945
Acc65I GGTACC 1 cut(s) 1928
AccB1I GGYRCC 1 cut(s) 1928
AccB7I CCANNNNNTGG 1 cut(s) 361
AccBSI CCGCTC 1 cut(s) 1798
AccI GTMKAC 1 cut(s) 274
AccII CGCG 4 cut(s) 100, 1221, 3066, 3068
AciI CCGC 7 cut(s) 458, 1024, 1798, 1845, 1920, 1988, 2503
AcoI YGGCCR 1 cut(s) 2187
AcsI RAATTY 7 cut(s) 398, 968, 1259, 1493, 1787, 2215, 2597
AcuI CTGAAG 5 cut(s) 1845, 1854, 2646, 2690, 2877
AcyI GRCGYC 1 cut(s) 2694
AfiI CCNNNNNNNGG 7 cut(s) 361, 1743, 1925, 2010, 2156, 2512, 2749
AflII CTTAAG 2 cut(s) 1235, 1738
AhdI GACNNNNNGTC 1 cut(s) 270
AhlI ACTAGT 1 cut(s) 2440
AjnI CCWGG 8 cut(s) 690, 734, 741, 1519, 2149, 2758, 2974, 3040
AjuI GAANNNNNNNTTGG 4 cut(s) 234, 266, 558, 590
AloI GAACNNNNNNTCC 2 cut(s) 2334, 2366
Alw21I GWGCWC 5 cut(s) 84, 1126, 2436, 2974, 3049
Alw26I GTCTC 7 cut(s) 217, 1057, 1451, 2022, 2444, 2582, 3066
Alw44I GTGCAC 1 cut(s) 2970
AlwNI CAGNNNCTG 1 cut(s) 2621
Ama87I CYCGRG 2 cut(s) 83, 1793
AoxI GGCC 5 cut(s) 7, 537, 1517, 2107, 2187
ApaLI GTGCAC 1 cut(s) 2970
ApeKI GCWGC 7 cut(s) 447, 476, 1241, 1721, 1879, 3023, 3026
ApoI RAATTY 7 cut(s) 398, 968, 1259, 1493, 1787, 2215, 2597
Asp700I GAANNNNTTC 2 cut(s) 2250, 2927
Asp718I GGTACC 1 cut(s) 1928
AspA2I CCTAGG 1 cut(s) 2462
AspLEI GCGC 8 cut(s) 102, 229, 495, 613, 1221, 2331, 3068, 3070
AspS9I GGNCC 6 cut(s) 537, 1416, 2090, 2108, 2660, 2978
AsuC2I CCSGG 1 cut(s) 2115
AsuHPI GGTGA 8 cut(s) 617, 1075, 1493, 1763, 2055, 2090, 2174, 2370
AvaI CYCGRG 2 cut(s) 83, 1793
AvaII GGWCC 4 cut(s) 1416, 2090, 2660, 2978
AvrII CCTAGG 1 cut(s) 2462
BaeGI GKGCMC 4 cut(s) 1876, 2470, 2483, 2974
BaeI ACNNNNGTAYC 8 cut(s) 831, 864, 1175, 1208, 1821, 1854, 2109, 2142
BamHI GGATCC 3 cut(s) 1096, 2899, 3037
BanI GGYRCC 1 cut(s) 1928
BanII GRGCYC 2 cut(s) 84, 2436
BauI CACGAG 1 cut(s) 2967
BbsI GAAGAC 2 cut(s) 641, 1383
Bbv12I GWGCWC 5 cut(s) 84, 1126, 2436, 2974, 3049
BbvI GCAGC 7 cut(s) 459, 463, 1228, 1708, 1891, 3010, 3038
BccI CCATC 3 cut(s) 524, 736, 829
BceAI ACGGC 6 cut(s) 692, 1532, 1532, 2108, 2202, 3160
BciT130I CCWGG 8 cut(s) 692, 736, 743, 1521, 2151, 2760, 2976, 3042
BciVI GTATCC 2 cut(s) 246, 1585
BclI TGATCA 1 cut(s) 1812
BcnI CCSGG 1 cut(s) 2115
BcoDI GTCTC 7 cut(s) 217, 1057, 1451, 2022, 2444, 2582, 3066
BcuI ACTAGT 1 cut(s) 2440
BfaI CTAG 5 cut(s) 444, 671, 2441, 2463, 2841
BfmI CTRYAG 7 cut(s) 451, 764, 1187, 1652, 2229, 2958, 3024
BfoI RGCGCY 2 cut(s) 230, 2332
BfrI CTTAAG 2 cut(s) 1235, 1738
BfuAI ACCTGC 4 cut(s) 238, 1140, 1453, 1945
BfuI GTATCC 2 cut(s) 246, 1585
BglII AGATCT 1 cut(s) 1617
BisI GCNGC 8 cut(s) 448, 477, 1242, 1722, 1799, 1880, 3024, 3027
BlnI CCTAGG 1 cut(s) 2462
BlsI GCNGC 8 cut(s) 449, 478, 1243, 1723, 1800, 1881, 3025, 3028
Bme1390I CCNGG 9 cut(s) 692, 736, 743, 1521, 2115, 2151, 2760, 2976, 3042
Bme18I GGWCC 4 cut(s) 1416, 2090, 2660, 2978
BmeRI GACNNNNNGTC 1 cut(s) 270
BmeT110I CYCGRG 2 cut(s) 83, 1793
BmgT120I GGNCC 6 cut(s) 537, 1416, 2090, 2108, 2660, 2978
BmiI GGNNCC 9 cut(s) 65, 689, 740, 1098, 1346, 1930, 2091, 2901, 3039
BmrFI CCNGG 9 cut(s) 692, 736, 743, 1521, 2115, 2151, 2760, 2976, 3042
BmrI ACTGGG 1 cut(s) 2866
BmsI GCATC 9 cut(s) 622, 732, 884, 913, 925, 1704, 2170, 2817, 3091
BmuI ACTGGG 1 cut(s) 2866
BoxI GACNNNNGTC 1 cut(s) 1914
BpiI GAAGAC 2 cut(s) 641, 1383
BpmI CTGGAG 4 cut(s) 661, 725, 1623, 2982
BpuMI CCSGG 1 cut(s) 2115
Bsa29I ATCGAT 1 cut(s) 1698
BsaHI GRCGYC 1 cut(s) 2694
BsaI GGTCTC 1 cut(s) 3066
BsaJI CCNNGG 6 cut(s) 734, 1520, 2074, 2149, 2462, 2759
BsaWI WCCGGW 2 cut(s) 2065, 2742
Bsc4I CCNNNNNNNGG 7 cut(s) 361, 1743, 1925, 2010, 2156, 2512, 2749
Bse1I ACTGG 7 cut(s) 644, 2100, 2615, 2872, 2981, 2999, 3015
Bse3DI GCAATG 1 cut(s) 1221
BseBI CCWGG 8 cut(s) 692, 736, 743, 1521, 2151, 2760, 2976, 3042
BseCI ATCGAT 1 cut(s) 1698
BseDI CCNNGG 6 cut(s) 734, 1520, 2074, 2149, 2462, 2759
BseGI GGATG 8 cut(s) 723, 850, 1048, 2185, 2348, 2521, 2952, 3130
BseLI CCNNNNNNNGG 7 cut(s) 361, 1743, 1925, 2010, 2156, 2512, 2749
BseMI GCAATG 1 cut(s) 1221
BseMII CTCAG 5 cut(s) 290, 918, 2033, 2654, 2823
BseNI ACTGG 7 cut(s) 644, 2100, 2615, 2872, 2981, 2999, 3015
BsePI GCGCGC 1 cut(s) 3066
BseRI GAGGAG 1 cut(s) 2955
BseSI GKGCMC 4 cut(s) 1876, 2470, 2483, 2974
BseXI GCAGC 7 cut(s) 459, 463, 1228, 1708, 1891, 3010, 3038
BseYI CCCAGC 4 cut(s) 534, 1875, 2581, 3056
BsgI GTGCAG 2 cut(s) 462, 3148
Bsh1236I CGCG 4 cut(s) 100, 1221, 3066, 3068
Bsh1285I CGRYCG 1 cut(s) 544
BshFI GGCC 5 cut(s) 9, 539, 1519, 2109, 2189
BshNI GGYRCC 1 cut(s) 1928
BshVI ATCGAT 1 cut(s) 1698
BsiEI CGRYCG 1 cut(s) 544
BsiHKAI GWGCWC 5 cut(s) 84, 1126, 2436, 2974, 3049
BsiHKCI CYCGRG 2 cut(s) 83, 1793
BsiSI CCGG 3 cut(s) 2066, 2114, 2743
BsiWI CGTACG 1 cut(s) 95
BslFI GGGAC 2 cut(s) 928, 1902
BslI CCNNNNNNNGG 7 cut(s) 361, 1743, 1925, 2010, 2156, 2512, 2749
BsmAI GTCTC 7 cut(s) 217, 1057, 1451, 2022, 2444, 2582, 3066
BsmBI CGTCTC 1 cut(s) 2582
BsmFI GGGAC 2 cut(s) 928, 1902
BsmI GAATGC 1 cut(s) 1110
BsnI GGCC 5 cut(s) 9, 539, 1519, 2109, 2189
Bso31I GGTCTC 1 cut(s) 3066
BsoBI CYCGRG 2 cut(s) 83, 1793
Bsp1286I GDGCHC 8 cut(s) 84, 1126, 1876, 2436, 2470, 2483, 2974, 3049
BspACI CCGC 7 cut(s) 458, 1024, 1798, 1845, 1920, 1988, 2503
BspANI GGCC 5 cut(s) 9, 539, 1519, 2109, 2189
BspCNI CTCAG 5 cut(s) 291, 919, 2032, 2653, 2822
BspDI ATCGAT 1 cut(s) 1698
BspFNI CGCG 4 cut(s) 100, 1221, 3066, 3068
BspLI GGNNCC 9 cut(s) 65, 689, 740, 1098, 1346, 1930, 2091, 2901, 3039
BspMAI CTGCAG 3 cut(s) 768, 1191, 3028
BspMI ACCTGC 4 cut(s) 238, 1140, 1453, 1945
BspQI GCTCTTC 1 cut(s) 2202
BspT107I GGYRCC 1 cut(s) 1928
BspTI CTTAAG 2 cut(s) 1235, 1738
BspTNI GGTCTC 1 cut(s) 3066
BsrBI CCGCTC 1 cut(s) 1798
BsrDI GCAATG 1 cut(s) 1221
BsrI ACTGG 7 cut(s) 644, 2100, 2615, 2872, 2981, 2999, 3015
BssECI CCNNGG 6 cut(s) 734, 1520, 2074, 2149, 2462, 2759
BssHII GCGCGC 1 cut(s) 3066
BssNI GRCGYC 1 cut(s) 2694
BssSI CACGAG 1 cut(s) 2967
BssT1I CCWWGG 2 cut(s) 2074, 2462
Bst2BI CACGAG 1 cut(s) 2967
Bst2UI CCWGG 8 cut(s) 692, 736, 743, 1521, 2151, 2760, 2976, 3042
Bst4CI ACNGT 5 cut(s) 319, 781, 1573, 2478, 2887
Bst6I CTCTTC 2 cut(s) 2202, 2622
BstACI GRCGYC 1 cut(s) 2694
BstAFI CTTAAG 2 cut(s) 1235, 1738
BstAPI GCANNNNNTGC 1 cut(s) 2180
BstC8I GCNNGC 7 cut(s) 681, 1028, 1122, 1872, 2794, 3054, 3068
BstDEI CTNAG 7 cut(s) 299, 927, 1196, 1614, 2019, 2640, 2809
BstEII GGTNACC 3 cut(s) 1499, 2078, 2162
BstENI CCTNNNNNAGG 1 cut(s) 1741
BstF5I GGATG 8 cut(s) 723, 850, 1048, 2185, 2348, 2521, 2952, 3130
BstFNI CGCG 4 cut(s) 100, 1221, 3066, 3068
BstH2I RGCGCY 2 cut(s) 230, 2332
BstHHI GCGC 8 cut(s) 102, 229, 495, 613, 1221, 2331, 3068, 3070
BstMAI GTCTC 7 cut(s) 217, 1057, 1451, 2022, 2444, 2582, 3066
BstMCI CGRYCG 1 cut(s) 544
BstMWI GCNNNNNNNGC 9 cut(s) 61, 881, 1211, 1250, 1876, 2180, 2720, 2969, 3053
BstNI CCWGG 8 cut(s) 692, 736, 743, 1521, 2151, 2760, 2976, 3042
BstNSI RCATGY 2 cut(s) 1030, 1147
BstPAI GACNNNNGTC 1 cut(s) 1914
BstPI GGTNACC 3 cut(s) 1499, 2078, 2162
BstSCI CCNGG 9 cut(s) 690, 734, 741, 1519, 2113, 2149, 2758, 2974, 3040
BstSFI CTRYAG 7 cut(s) 451, 764, 1187, 1652, 2229, 2958, 3024
BstSLI GKGCMC 4 cut(s) 1876, 2470, 2483, 2974
BstUI CGCG 4 cut(s) 100, 1221, 3066, 3068
BstV1I GCAGC 7 cut(s) 459, 463, 1228, 1708, 1891, 3010, 3038
BstV2I GAAGAC 2 cut(s) 641, 1383
BstXI CCANNNNNNTGG 2 cut(s) 735, 1952
Bsu15I ATCGAT 1 cut(s) 1698
BsuI GTATCC 2 cut(s) 246, 1585
BsuRI GGCC 5 cut(s) 9, 539, 1519, 2109, 2189
BsuTUI ATCGAT 1 cut(s) 1698
BtgZI GCGATG 1 cut(s) 2840
BtsCI GGATG 8 cut(s) 723, 850, 1048, 2185, 2348, 2521, 2952, 3130
BtsI GCAGTG 2 cut(s) 2181, 2702
BtsIMutI CAGTG 8 cut(s) 777, 1480, 2093, 2181, 2483, 2648, 2702, 2988
BveI ACCTGC 4 cut(s) 238, 1140, 1453, 1945
Cac8I GCNNGC 7 cut(s) 681, 1028, 1122, 1872, 2794, 3054, 3068
CaiI CAGNNNCTG 1 cut(s) 2621
CfoI GCGC 8 cut(s) 102, 229, 495, 613, 1221, 2331, 3068, 3070
Cfr13I GGNCC 6 cut(s) 537, 1416, 2090, 2108, 2660, 2978
ClaI ATCGAT 1 cut(s) 1698
CseI GACGC 1 cut(s) 2683
CspCI CAANNNNNGTGG 2 cut(s) 2157, 2192
DdeI CTNAG 7 cut(s) 299, 927, 1196, 1614, 2019, 2640, 2809
DraI TTTAAA 1 cut(s) 2425
DrdI GACNNNNNNGTC 1 cut(s) 1385
DriI GACNNNNNGTC 1 cut(s) 270
DseDI GACNNNNNNGTC 1 cut(s) 1385
EaeI YGGCCR 1 cut(s) 2187
Eam1104I CTCTTC 2 cut(s) 2202, 2622
Eam1105I GACNNNNNGTC 1 cut(s) 270
EarI CTCTTC 2 cut(s) 2202, 2622
EciI GGCGGA 1 cut(s) 2492
Ecl136II GAGCTC 2 cut(s) 82, 2434
Eco130I CCWWGG 2 cut(s) 2074, 2462
Eco147I AGGCCT 1 cut(s) 9
Eco24I GRGCYC 2 cut(s) 84, 2436
Eco31I GGTCTC 1 cut(s) 3066
Eco32I GATATC 2 cut(s) 1001, 1054
Eco47I GGWCC 4 cut(s) 1416, 2090, 2660, 2978
Eco53kI GAGCTC 2 cut(s) 82, 2434
Eco57I CTGAAG 5 cut(s) 1845, 1854, 2646, 2690, 2877
Eco88I CYCGRG 2 cut(s) 83, 1793
Eco91I GGTNACC 3 cut(s) 1499, 2078, 2162
EcoICRI GAGCTC 2 cut(s) 82, 2434
EcoNI CCTNNNNNAGG 1 cut(s) 1741
EcoO109I RGGNCCY 1 cut(s) 2108
EcoO65I GGTNACC 3 cut(s) 1499, 2078, 2162
EcoRII CCWGG 8 cut(s) 690, 734, 741, 1519, 2149, 2758, 2974, 3040
EcoRV GATATC 2 cut(s) 1001, 1054
EcoT14I CCWWGG 2 cut(s) 2074, 2462
EcoT22I ATGCAT 1 cut(s) 918
EcoT38I GRGCYC 2 cut(s) 84, 2436
ErhI CCWWGG 2 cut(s) 2074, 2462
Esp3I CGTCTC 1 cut(s) 2582
FaqI GGGAC 2 cut(s) 928, 1902
FauI CCCGC 2 cut(s) 1031, 1927
FauNDI CATATG 2 cut(s) 130, 1609
FbaI TGATCA 1 cut(s) 1812
FblI GTMKAC 1 cut(s) 274
Fnu4HI GCNGC 8 cut(s) 448, 477, 1242, 1722, 1799, 1880, 3024, 3027
FokI GGATG 8 cut(s) 710, 857, 1055, 2192, 2355, 2528, 2939, 3137
FriOI GRGCYC 2 cut(s) 84, 2436
Fsp4HI GCNGC 8 cut(s) 448, 477, 1242, 1722, 1799, 1880, 3024, 3027
FspBI CTAG 5 cut(s) 444, 671, 2441, 2463, 2841
GlaI GCGC 8 cut(s) 101, 228, 494, 612, 1220, 2330, 3067, 3069
GluI GCNGC 8 cut(s) 448, 477, 1242, 1722, 1799, 1880, 3024, 3027
GsaI CCCAGC 4 cut(s) 538, 1879, 2585, 3060
GsuI CTGGAG 4 cut(s) 661, 725, 1623, 2982
HaeII RGCGCY 2 cut(s) 230, 2332
HaeIII GGCC 5 cut(s) 9, 539, 1519, 2109, 2189
HapII CCGG 3 cut(s) 2066, 2114, 2743
HgaI GACGC 1 cut(s) 2683
HhaI GCGC 8 cut(s) 102, 229, 495, 613, 1221, 2331, 3068, 3070
Hin1I GRCGYC 1 cut(s) 2694
Hin6I GCGC 8 cut(s) 100, 227, 493, 611, 1219, 2329, 3066, 3068
HinP1I GCGC 8 cut(s) 100, 227, 493, 611, 1219, 2329, 3066, 3068
HincII GTYRAC 2 cut(s) 2910, 2950
HindII GTYRAC 2 cut(s) 2910, 2950
HindIII AAGCTT 3 cut(s) 1562, 2570, 2672
HinfI GANTC 6 cut(s) 407, 1294, 1657, 1708, 2497, 3117
HpaI GTTAAC 2 cut(s) 2910, 2950
HpaII CCGG 3 cut(s) 2066, 2114, 2743
HphI GGTGA 8 cut(s) 617, 1075, 1493, 1763, 2055, 2090, 2174, 2370
Hpy166II GTNNAC 8 cut(s) 275, 711, 2093, 2819, 2910, 2950, 2972, 3142
Hpy188I TCNGA 9 cut(s) 36, 300, 1391, 1662, 2307, 2312, 2670, 2812, 3116
Hpy8I GTNNAC 8 cut(s) 275, 711, 2093, 2819, 2910, 2950, 2972, 3142
HpyAV CCTTC 6 cut(s) 611, 925, 1087, 2070, 2099, 2917
HpyCH4III ACNGT 5 cut(s) 319, 781, 1573, 2478, 2887
HpyCH4IV ACGT 4 cut(s) 568, 826, 1915, 2906
HpyF10VI GCNNNNNNNGC 9 cut(s) 61, 881, 1211, 1250, 1876, 2180, 2720, 2969, 3053
HpyF3I CTNAG 7 cut(s) 299, 927, 1196, 1614, 2019, 2640, 2809
HpySE526I ACGT 4 cut(s) 568, 826, 1915, 2906
Hsp92I GRCGYC 1 cut(s) 2694
HspAI GCGC 8 cut(s) 100, 227, 493, 611, 1219, 2329, 3066, 3068
KpnI GGTACC 1 cut(s) 1932
Ksp22I TGATCA 1 cut(s) 1812
KspAI GTTAAC 2 cut(s) 2910, 2950
LguI GCTCTTC 1 cut(s) 2202
LmnI GCTCC 6 cut(s) 69, 79, 744, 1270, 2431, 2968
Lsp1109I GCAGC 7 cut(s) 459, 463, 1228, 1708, 1891, 3010, 3038
LweI GCATC 9 cut(s) 622, 732, 884, 913, 925, 1704, 2170, 2817, 3091
MaeI CTAG 5 cut(s) 444, 671, 2441, 2463, 2841
MaeII ACGT 4 cut(s) 568, 826, 1915, 2906
MbiI CCGCTC 1 cut(s) 1798
MfeI CAATTG 2 cut(s) 1301, 2100
MhlI GDGCHC 8 cut(s) 84, 1126, 1876, 2436, 2470, 2483, 2974, 3049
MlyI GAGTC 1 cut(s) 416
MmeI TCCRAC 1 cut(s) 3094
Mph1103I ATGCAT 1 cut(s) 918
MroXI GAANNNNTTC 2 cut(s) 2250, 2927
MslI CAYNNNNRTG 4 cut(s) 170, 630, 921, 1031
MspA1I CMGCKG 2 cut(s) 2585, 3023
MspCI CTTAAG 2 cut(s) 1235, 1738
MspI CCGG 3 cut(s) 2066, 2114, 2743
MspR9I CCNGG 9 cut(s) 692, 736, 743, 1521, 2115, 2151, 2760, 2976, 3042
MunI CAATTG 2 cut(s) 1301, 2100
Mva1269I GAATGC 1 cut(s) 1110
MvaI CCWGG 8 cut(s) 692, 736, 743, 1521, 2151, 2760, 2976, 3042
MvnI CGCG 4 cut(s) 100, 1221, 3066, 3068
MwoI GCNNNNNNNGC 9 cut(s) 61, 881, 1211, 1250, 1876, 2180, 2720, 2969, 3053
NciI CCSGG 1 cut(s) 2115
NdeI CATATG 2 cut(s) 130, 1609
NlaIV GGNNCC 9 cut(s) 65, 689, 740, 1098, 1346, 1930, 2091, 2901, 3039
NmuCI GTSAC 8 cut(s) 197, 262, 1230, 1499, 1903, 2078, 2162, 2549
NsiI ATGCAT 1 cut(s) 918
NspI RCATGY 2 cut(s) 1030, 1147
PaeI GCATGC 1 cut(s) 1030
PaqCI CACCTGC 1 cut(s) 1945
PauI GCGCGC 1 cut(s) 3066
PceI AGGCCT 1 cut(s) 9
PciSI GCTCTTC 1 cut(s) 2202
PcsI WCGNNNNNNNCGW 1 cut(s) 1218
PctI GAATGC 1 cut(s) 1110
PdmI GAANNNNTTC 2 cut(s) 2250, 2927
PfeI GAWTC 5 cut(s) 1294, 1657, 1708, 2497, 3117
Pfl23II CGTACG 1 cut(s) 95
PflMI CCANNNNNTGG 1 cut(s) 361
PkrI GCNGC 8 cut(s) 449, 478, 1243, 1723, 1800, 1881, 3025, 3028
Ple19I CGATCG 1 cut(s) 544
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
PshAI GACNNNNGTC 1 cut(s) 1914
Psp124BI GAGCTC 2 cut(s) 84, 2436
Psp6I CCWGG 8 cut(s) 690, 734, 741, 1519, 2149, 2758, 2974, 3040
PspEI GGTNACC 3 cut(s) 1499, 2078, 2162
PspFI CCCAGC 4 cut(s) 534, 1875, 2581, 3056
PspGI CCWGG 8 cut(s) 690, 734, 741, 1519, 2149, 2758, 2974, 3040
PspLI CGTACG 1 cut(s) 95
PspN4I GGNNCC 9 cut(s) 65, 689, 740, 1098, 1346, 1930, 2091, 2901, 3039
PspPI GGNCC 6 cut(s) 537, 1416, 2090, 2108, 2660, 2978
PstI CTGCAG 3 cut(s) 768, 1191, 3028
PstNI CAGNNNCTG 1 cut(s) 2621
PteI GCGCGC 1 cut(s) 3066
PvuI CGATCG 1 cut(s) 544
PvuII CAGCTG 2 cut(s) 2585, 3023
RseI CAYNNNNRTG 4 cut(s) 170, 630, 921, 1031
SacI GAGCTC 2 cut(s) 84, 2436
SapI GCTCTTC 1 cut(s) 2202
SatI GCNGC 8 cut(s) 448, 477, 1242, 1722, 1799, 1880, 3024, 3027
Sau96I GGNCC 6 cut(s) 537, 1416, 2090, 2108, 2660, 2978
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 9 cut(s) 692, 736, 743, 1521, 2115, 2151, 2760, 2976, 3042
SduI GDGCHC 8 cut(s) 84, 1126, 1876, 2436, 2470, 2483, 2974, 3049
SfaNI GCATC 9 cut(s) 622, 732, 884, 913, 925, 1704, 2170, 2817, 3091
SfcI CTRYAG 7 cut(s) 451, 764, 1187, 1652, 2229, 2958, 3024
SinI GGWCC 4 cut(s) 1416, 2090, 2660, 2978
SmiMI CAYNNNNRTG 4 cut(s) 170, 630, 921, 1031
SmlI CTYRAG 2 cut(s) 1235, 1738
SmoI CTYRAG 2 cut(s) 1235, 1738
SpeI ACTAGT 1 cut(s) 2440
SphI GCATGC 1 cut(s) 1030
SseBI AGGCCT 1 cut(s) 9
SsiI CCGC 7 cut(s) 458, 1024, 1798, 1845, 1920, 1988, 2503
SspI AATATT 5 cut(s) 385, 994, 1674, 2239, 2302
SspMI CTAG 5 cut(s) 444, 671, 2441, 2463, 2841
SstI GAGCTC 2 cut(s) 84, 2436
StuI AGGCCT 1 cut(s) 9
StyD4I CCNGG 9 cut(s) 690, 734, 741, 1519, 2113, 2149, 2758, 2974, 3040
StyI CCWWGG 2 cut(s) 2074, 2462
TaaI ACNGT 5 cut(s) 319, 781, 1573, 2478, 2887
TaiI ACGT 4 cut(s) 571, 829, 1918, 2909
TaqI TCGA 3 cut(s) 72, 997, 1698
TaqII GACCGA 2 cut(s) 1922, 2537
TatI WGTACW 3 cut(s) 708, 1199, 2816
TauI GCSGC 1 cut(s) 1801
TfiI GAWTC 5 cut(s) 1294, 1657, 1708, 2497, 3117
TscAI CASTG 8 cut(s) 784, 1480, 2100, 2181, 2483, 2648, 2709, 2988
TseFI GTSAC 8 cut(s) 197, 262, 1230, 1499, 1903, 2078, 2162, 2549
TseI GCWGC 7 cut(s) 447, 476, 1241, 1721, 1879, 3023, 3026
Tsp45I GTSAC 8 cut(s) 197, 262, 1230, 1499, 1903, 2078, 2162, 2549
TspDTI ATGAA 8 cut(s) 10, 297, 360, 579, 1389, 2243, 2304, 2920
TspGWI ACGGA 6 cut(s) 56, 337, 857, 1055, 1337, 3050
TspRI CASTG 8 cut(s) 784, 1480, 2100, 2181, 2483, 2648, 2709, 2988
Van91I CCANNNNNTGG 1 cut(s) 361
Vha464I CTTAAG 2 cut(s) 1235, 1738
VneI GTGCAC 1 cut(s) 2970
VpaK11BI GGWCC 4 cut(s) 1416, 2090, 2660, 2978
XagI CCTNNNNNAGG 1 cut(s) 1741
XapI RAATTY 7 cut(s) 398, 968, 1259, 1493, 1787, 2215, 2597
XceI RCATGY 2 cut(s) 1030, 1147
XmaJI CCTAGG 1 cut(s) 2462
XmiI GTMKAC 1 cut(s) 274
XmnI GAANNNNTTC 2 cut(s) 2250, 2927
XspI CTAG 5 cut(s) 444, 671, 2441, 2463, 2841
Zsp2I ATGCAT 1 cut(s) 918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.