Rmu_ssc0000029.1_g000011

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000029.1
OG0013881
Physical Location & Seq
Forward (+)
56040 .. 64873
8834 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1227 bp
atgagatacagcactagtttgattttgatcaaactattggtaattcaatacctgtcagttctttgcgctacgcaggactttgatttcttctacttcgttcaacagtggcctggagcatattgcgacacaaagcatagttgttgctatccaaagtcagggaagcctgcagcagatttcggcattcacggtctctggccaaactacaaggatggctcttacccttcaaactgtgatccagacagtgtcttcgacaaatctgagatctcagagttgatgggcagtctggaaaagagttggccatctatgagctgcccaagcagtaatggttacaggttctggtcacacgaatgggagaagcatggcacctgctccgaatctgaacttgatcagaaagattacttccaagccggtctcaagctcaaggaaaaagcaaaccttcttcaagcccttaaaaaagctggaataaaaccagacgatgaactatacagcttagaaagcattgtagcagctataaaagagggtgttgggcatgccccagggattgagtgcaacaaggattcagctggtaacaaccaactttaccaagtttacttcaggtggctatggcggctcggggcttgcaggagggaggccggaggcgtccaggccggttctgggcaccgtttgaggtcgggcttgccggtttctaggcaggcacctcacctgcaactgcaggttctgtgcagcacagcaaaacccgtcgccggtgactccacccgacagcagaccctggcctccctccgtcgcggctcgatcgaggccggagcagcgacgtccctccacctcgaccacaagcatccaattccaaatcttatgatcccaatccaaacgggagctcctccaaattgcaacttgacctttcttttatggtttcccaccgttaacgcggaggttcaaacacctgcgatcccccacttagagtctctctctctctctctctgcattccggtttctttctccgatttccggcattcttccgccggagctgcctcactactaacttttcaaactacgagactacaagttacttccggctctcgattttgctccgccactggtttcctcgccgggaaagttgaagaagatatcggaattaagctcagcgtctccatgctggtttcagtaccaaaacgacgacaaggtaactacttctctgaacttccgagcttcgatgattttcaattttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

408

Amino Acids

45.07

Weight (kDa)

7.54

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 27 - 198 1.1e-59 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013881)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14220 AT1G26820
fragaria_vesca FvH4_4g31290
malus_domestica MD13G1071500.v1.1
prunus_persica Prupe.1G275400_v2.0.a1
pyrus_communis pycom13g06410
rosa_chinensis RchiOBHm_Chr4g0439731
rosa_laevigata RLG00000006241
rosa_multiflora Rmu_sc0010226.1_g000016 Rmu_ssc0000029.1_g000011
rosa_roxburghii Rroxscaffold_5G00380670
rosa_rugosa Rorug04G0321100
rosa_samantha Rh4AG372100 Rh4BG384200 Rh4CG398900 Rh4DG378600
rosa_wichuraiana Rw4G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 374, 711, 949
AatII GACGTC 1 cut(s) 815
Acc36I ACCTGC 4 cut(s) 374, 703, 711, 949
AccB1I GGYRCC 3 cut(s) 362, 657, 694
AccII CGCG 2 cut(s) 786, 926
AciI CCGC 5 cut(s) 607, 786, 926, 1017, 1089
AclWI GGATC 3 cut(s) 227, 850, 940
AcoI YGGCCR 2 cut(s) 194, 296
AcuI CTGAAG 1 cut(s) 577
AcyI GRCGYC 2 cut(s) 639, 812
AfaI GTAC 1 cut(s) 1164
AfiI CCNNNNNNNGG 4 cut(s) 155, 654, 743, 1005
AgsI TTSAA 8 cut(s) 47, 101, 225, 443, 935, 1046, 1118, 1220
AhlI ACTAGT 1 cut(s) 14
AjnI CCWGG 4 cut(s) 109, 535, 642, 768
Alw21I GWGCWC 1 cut(s) 877
Alw26I GTCTC 5 cut(s) 194, 416, 966, 1048, 1150
AlwI GGATC 3 cut(s) 227, 850, 940
AlwNI CAGNNNCTG 3 cut(s) 336, 718, 769
Ama87I CYCGRG 1 cut(s) 611
AoxI GGCC 7 cut(s) 107, 194, 296, 630, 645, 771, 798
ApeKI GCWGC 6 cut(s) 167, 309, 506, 723, 806, 1025
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AspLEI GCGC 1 cut(s) 68
AsuC2I CCSGG 1 cut(s) 1108
AsuHPI GGTGA 2 cut(s) 692, 758
AvaI CYCGRG 1 cut(s) 611
BaeGI GKGCMC 1 cut(s) 660
BalI TGGCCA 2 cut(s) 196, 298
BanI GGYRCC 3 cut(s) 362, 657, 694
BanII GRGCYC 1 cut(s) 877
BbsI GAAGAC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 877
BbvI GCAGC 6 cut(s) 179, 296, 518, 735, 818, 1012
BccI CCATC 3 cut(s) 203, 268, 307
BciT130I CCWGG 4 cut(s) 111, 537, 644, 770
BclI TGATCA 2 cut(s) 27, 385
BcnI CCSGG 1 cut(s) 1108
BcoDI GTCTC 5 cut(s) 194, 416, 966, 1048, 1150
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 2 cut(s) 15, 687
BfmI CTRYAG 2 cut(s) 165, 710
BfuAI ACCTGC 4 cut(s) 374, 703, 711, 949
BglII AGATCT 1 cut(s) 261
BisI GCNGC 8 cut(s) 168, 310, 507, 608, 724, 787, 807, 1026
BlpI GCTNAGC 1 cut(s) 1139
BlsI GCNGC 8 cut(s) 169, 311, 508, 609, 725, 788, 808, 1027
Bme1390I CCNGG 5 cut(s) 111, 537, 644, 770, 1108
BmeT110I CYCGRG 1 cut(s) 611
BmiI GGNNCC 3 cut(s) 364, 659, 696
BmrFI CCNGG 5 cut(s) 111, 537, 644, 770, 1108
BmsI GCATC 1 cut(s) 844
BpiI GAAGAC 1 cut(s) 238
BplI GAGNNNNNCTC 2 cut(s) 950, 982
BpmI CTGGAG 1 cut(s) 132
Bpu1102I GCTNAGC 1 cut(s) 1139
BpuEI CTTGAG 2 cut(s) 398, 404
BpuMI CCSGG 1 cut(s) 1108
BsaBI GATNNNNATC 2 cut(s) 26, 860
BsaHI GRCGYC 2 cut(s) 639, 812
BsaI GGTCTC 2 cut(s) 194, 416
BsaJI CCNNGG 3 cut(s) 535, 536, 768
BsaWI WCCGGW 1 cut(s) 985
BsaXI ACNNNNNCTCC 2 cut(s) 859, 889
Bsc4I CCNNNNNNNGG 4 cut(s) 155, 654, 743, 1005
Bse118I RCCGGY 4 cut(s) 407, 647, 679, 743
Bse1I ACTGG 1 cut(s) 1099
Bse8I GATNNNNATC 2 cut(s) 26, 860
BseBI CCWGG 4 cut(s) 111, 537, 644, 770
BseDI CCNNGG 3 cut(s) 535, 536, 768
BseGI GGATG 2 cut(s) 214, 835
BseJI GATNNNNATC 2 cut(s) 26, 860
BseLI CCNNNNNNNGG 4 cut(s) 155, 654, 743, 1005
BseMII CTCAG 3 cut(s) 249, 279, 1153
BseNI ACTGG 1 cut(s) 1099
BseRI GAGGAG 1 cut(s) 867
BseSI GKGCMC 1 cut(s) 660
BseXI GCAGC 6 cut(s) 179, 296, 518, 735, 818, 1012
BsgI GTGCAG 1 cut(s) 742
Bsh1236I CGCG 2 cut(s) 786, 926
Bsh1285I CGRYCG 1 cut(s) 795
BshFI GGCC 7 cut(s) 109, 196, 298, 632, 647, 773, 800
BshNI GGYRCC 3 cut(s) 362, 657, 694
BsiEI CGRYCG 1 cut(s) 795
BsiHKAI GWGCWC 1 cut(s) 877
BsiHKCI CYCGRG 1 cut(s) 611
BslFI GGGAC 1 cut(s) 799
BslI CCNNNNNNNGG 4 cut(s) 155, 654, 743, 1005
BsmAI GTCTC 5 cut(s) 194, 416, 966, 1048, 1150
BsmBI CGTCTC 1 cut(s) 1150
BsmFI GGGAC 1 cut(s) 799
BsmI GAATGC 3 cut(s) 180, 981, 1009
BsnI GGCC 7 cut(s) 109, 196, 298, 632, 647, 773, 800
Bso31I GGTCTC 2 cut(s) 194, 416
BsoBI CYCGRG 1 cut(s) 611
Bsp1286I GDGCHC 2 cut(s) 660, 877
Bsp143I GATC 7 cut(s) 27, 232, 261, 385, 792, 855, 945
Bsp1720I GCTNAGC 1 cut(s) 1139
BspACI CCGC 5 cut(s) 607, 786, 926, 1017, 1089
BspANI GGCC 7 cut(s) 109, 196, 298, 632, 647, 773, 800
BspCNI CTCAG 3 cut(s) 250, 278, 1152
BspFNI CGCG 2 cut(s) 786, 926
BspLI GGNNCC 3 cut(s) 364, 659, 696
BspMAI CTGCAG 2 cut(s) 169, 714
BspMI ACCTGC 4 cut(s) 374, 703, 711, 949
BspPI GGATC 3 cut(s) 227, 850, 940
BspT107I GGYRCC 3 cut(s) 362, 657, 694
BspTNI GGTCTC 2 cut(s) 194, 416
BsrFI RCCGGY 4 cut(s) 407, 647, 679, 743
BsrI ACTGG 1 cut(s) 1099
BssAI RCCGGY 4 cut(s) 407, 647, 679, 743
BssECI CCNNGG 3 cut(s) 535, 536, 768
BssMI GATC 7 cut(s) 27, 232, 261, 385, 792, 855, 945
BssNI GRCGYC 2 cut(s) 639, 812
Bst2UI CCWGG 4 cut(s) 111, 537, 644, 770
Bst4CI ACNGT 6 cut(s) 105, 188, 230, 242, 662, 919
BstACI GRCGYC 2 cut(s) 639, 812
BstC8I GCNNGC 5 cut(s) 165, 531, 619, 677, 693
BstDEI CTNAG 5 cut(s) 258, 265, 490, 955, 1139
BstF5I GGATG 2 cut(s) 214, 835
BstFNI CGCG 2 cut(s) 786, 926
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 7 cut(s) 30, 235, 264, 388, 795, 858, 948
BstMAI GTCTC 5 cut(s) 194, 416, 966, 1048, 1150
BstMBI GATC 7 cut(s) 27, 232, 261, 385, 792, 855, 945
BstMCI CGRYCG 1 cut(s) 795
BstMWI GCNNNNNNNGC 5 cut(s) 315, 495, 607, 806, 1025
BstNI CCWGG 4 cut(s) 111, 537, 644, 770
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 5 cut(s) 109, 535, 642, 768, 1106
BstSFI CTRYAG 2 cut(s) 165, 710
BstSLI GKGCMC 1 cut(s) 660
BstUI CGCG 2 cut(s) 786, 926
BstV1I GCAGC 6 cut(s) 179, 296, 518, 735, 818, 1012
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BsuRI GGCC 7 cut(s) 109, 196, 298, 632, 647, 773, 800
BtsCI GGATG 2 cut(s) 214, 835
BtsIMutI CAGTG 3 cut(s) 110, 247, 1092
BveI ACCTGC 4 cut(s) 374, 703, 711, 949
Cac8I GCNNGC 5 cut(s) 165, 531, 619, 677, 693
CaiI CAGNNNCTG 3 cut(s) 336, 718, 769
CfoI GCGC 1 cut(s) 68
Cfr10I RCCGGY 4 cut(s) 407, 647, 679, 743
CseI GACGC 2 cut(s) 628, 1132
Csp6I GTAC 1 cut(s) 1163
CviAII CATG 3 cut(s) 359, 530, 1150
CviQI GTAC 1 cut(s) 1163
DdeI CTNAG 5 cut(s) 258, 265, 490, 955, 1139
DpnI GATC 7 cut(s) 29, 234, 263, 387, 794, 857, 947
DpnII GATC 7 cut(s) 27, 232, 261, 385, 792, 855, 945
EaeI YGGCCR 2 cut(s) 194, 296
EciI GGCGGA 2 cut(s) 1006, 1078
Ecl136II GAGCTC 1 cut(s) 875
Eco24I GRGCYC 1 cut(s) 877
Eco31I GGTCTC 2 cut(s) 194, 416
Eco32I GATATC 1 cut(s) 1126
Eco53kI GAGCTC 1 cut(s) 875
Eco57I CTGAAG 1 cut(s) 577
Eco88I CYCGRG 1 cut(s) 611
EcoICRI GAGCTC 1 cut(s) 875
EcoRII CCWGG 4 cut(s) 109, 535, 642, 768
EcoRV GATATC 1 cut(s) 1126
EcoT38I GRGCYC 1 cut(s) 877
Esp3I CGTCTC 1 cut(s) 1150
FaeI CATG 3 cut(s) 362, 533, 1153
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 1 cut(s) 799
FatI CATG 3 cut(s) 358, 529, 1149
FbaI TGATCA 2 cut(s) 27, 385
Fnu4HI GCNGC 8 cut(s) 168, 310, 507, 608, 724, 787, 807, 1026
FokI GGATG 2 cut(s) 221, 822
FriOI GRGCYC 1 cut(s) 877
Fsp4HI GCNGC 8 cut(s) 168, 310, 507, 608, 724, 787, 807, 1026
FspBI CTAG 2 cut(s) 15, 687
GlaI GCGC 1 cut(s) 67
GluI GCNGC 8 cut(s) 168, 310, 507, 608, 724, 787, 807, 1026
GsuI CTGGAG 1 cut(s) 132
HaeIII GGCC 7 cut(s) 109, 196, 298, 632, 647, 773, 800
HgaI GACGC 2 cut(s) 628, 1132
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 2 cut(s) 639, 812
Hin1II CATG 3 cut(s) 362, 533, 1153
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HinfI GANTC 4 cut(s) 374, 557, 749, 959
HpaI GTTAAC 1 cut(s) 922
HphI GGTGA 2 cut(s) 692, 758
Hpy166II GTNNAC 2 cut(s) 589, 922
Hpy188I TCNGA 9 cut(s) 259, 268, 373, 379, 390, 1000, 1130, 1195, 1203
Hpy188III TCNNGA 3 cut(s) 236, 284, 1077
Hpy8I GTNNAC 2 cut(s) 589, 922
Hpy99I CGWCG 4 cut(s) 743, 786, 814, 1176
HpyAV CCTTC 2 cut(s) 231, 446
HpyCH4III ACNGT 6 cut(s) 105, 188, 230, 242, 662, 919
HpyCH4IV ACGT 1 cut(s) 812
HpyCH4V TGCA 8 cut(s) 167, 549, 621, 706, 712, 723, 888, 981
HpyF10VI GCNNNNNNNGC 5 cut(s) 315, 495, 607, 806, 1025
HpyF3I CTNAG 5 cut(s) 258, 265, 490, 955, 1139
HpySE526I ACGT 1 cut(s) 812
Hsp92I GRCGYC 2 cut(s) 639, 812
Hsp92II CATG 3 cut(s) 362, 533, 1153
HspAI GCGC 1 cut(s) 66
Ksp22I TGATCA 2 cut(s) 27, 385
KspAI GTTAAC 1 cut(s) 922
Kzo9I GATC 7 cut(s) 27, 232, 261, 385, 792, 855, 945
LmnI GCTCC 7 cut(s) 113, 374, 803, 872, 880, 1022, 1091
Lsp1109I GCAGC 6 cut(s) 179, 296, 518, 735, 818, 1012
LweI GCATC 1 cut(s) 844
MaeI CTAG 2 cut(s) 15, 687
MaeII ACGT 1 cut(s) 812
MaeIII GTNAC 6 cut(s) 326, 339, 566, 746, 1063, 1181
MalI GATC 7 cut(s) 29, 234, 263, 387, 794, 857, 947
MboI GATC 7 cut(s) 27, 232, 261, 385, 792, 855, 945
MboII GAAGA 6 cut(s) 79, 238, 431, 1005, 1130, 1133
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 2 cut(s) 660, 877
MlsI TGGCCA 2 cut(s) 196, 298
MluCI AATT 5 cut(s) 42, 840, 883, 1131, 1220
MluNI TGGCCA 2 cut(s) 196, 298
MlyI GAGTC 2 cut(s) 743, 968
Mox20I TGGCCA 2 cut(s) 196, 298
MscI TGGCCA 2 cut(s) 196, 298
MseI TTAA 3 cut(s) 450, 921, 1134
MslI CAYNNNNRTG 1 cut(s) 346
Msp20I TGGCCA 2 cut(s) 196, 298
MspA1I CMGCKG 1 cut(s) 563
MspR9I CCNGG 5 cut(s) 111, 537, 644, 770, 1108
Mva1269I GAATGC 3 cut(s) 180, 981, 1009
MvaI CCWGG 4 cut(s) 111, 537, 644, 770
MvnI CGCG 2 cut(s) 786, 926
MwoI GCNNNNNNNGC 5 cut(s) 315, 495, 607, 806, 1025
NciI CCSGG 1 cut(s) 1108
NdeII GATC 7 cut(s) 27, 232, 261, 385, 792, 855, 945
NlaIII CATG 3 cut(s) 362, 533, 1153
NlaIV GGNNCC 3 cut(s) 364, 659, 696
NmuCI GTSAC 2 cut(s) 339, 746
NspI RCATGY 1 cut(s) 533
PaeI GCATGC 1 cut(s) 533
PaqCI CACCTGC 3 cut(s) 374, 711, 949
PasI CCCWGGG 1 cut(s) 536
PctI GAATGC 3 cut(s) 180, 981, 1009
PfeI GAWTC 2 cut(s) 374, 557
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 8 cut(s) 169, 311, 508, 609, 725, 788, 808, 1027
Ple19I CGATCG 1 cut(s) 795
PleI GAGTC 2 cut(s) 743, 967
PpsI GAGTC 2 cut(s) 743, 967
Psp124BI GAGCTC 1 cut(s) 877
Psp6I CCWGG 4 cut(s) 109, 535, 642, 768
PspGI CCWGG 4 cut(s) 109, 535, 642, 768
PspN4I GGNNCC 3 cut(s) 364, 659, 696
PstI CTGCAG 2 cut(s) 169, 714
PstNI CAGNNNCTG 3 cut(s) 336, 718, 769
PsuI RGATCY 1 cut(s) 261
PsyI GACNNNGTC 1 cut(s) 242
PvuI CGATCG 1 cut(s) 795
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 1 cut(s) 1164
RsaNI GTAC 1 cut(s) 1163
RseI CAYNNNNRTG 1 cut(s) 346
SacI GAGCTC 1 cut(s) 877
SaqAI TTAA 3 cut(s) 450, 921, 1134
SatI GCNGC 8 cut(s) 168, 310, 507, 608, 724, 787, 807, 1026
Sau3AI GATC 7 cut(s) 27, 232, 261, 385, 792, 855, 945
SchI GAGTC 2 cut(s) 743, 968
ScrFI CCNGG 5 cut(s) 111, 537, 644, 770, 1108
SduI GDGCHC 2 cut(s) 660, 877
SfaNI GCATC 1 cut(s) 844
SfcI CTRYAG 2 cut(s) 165, 710
SgrAI CRCCGGYG 1 cut(s) 743
SmiMI CAYNNNNRTG 1 cut(s) 346
SmlI CTYRAG 2 cut(s) 413, 419
SmoI CTYRAG 2 cut(s) 413, 419
SpeI ACTAGT 1 cut(s) 14
SphI GCATGC 1 cut(s) 533
Sse9I AATT 5 cut(s) 42, 840, 883, 1131, 1220
SsiI CCGC 5 cut(s) 607, 786, 926, 1017, 1089
SspMI CTAG 2 cut(s) 15, 687
SstI GAGCTC 1 cut(s) 877
StyD4I CCNGG 5 cut(s) 109, 535, 642, 768, 1106
TaaI ACNGT 6 cut(s) 105, 188, 230, 242, 662, 919
TaiI ACGT 1 cut(s) 815
TaqI TCGA 6 cut(s) 249, 791, 795, 825, 1078, 1209
TasI AATT 5 cut(s) 42, 840, 883, 1131, 1220
TauI GCSGC 2 cut(s) 610, 789
TfiI GAWTC 2 cut(s) 374, 557
Tru1I TTAA 3 cut(s) 450, 921, 1134
Tru9I TTAA 3 cut(s) 450, 921, 1134
TscAI CASTG 3 cut(s) 110, 247, 1099
TseFI GTSAC 2 cut(s) 339, 746
TseI GCWGC 6 cut(s) 167, 309, 506, 723, 806, 1025
Tsp45I GTSAC 2 cut(s) 339, 746
TspDTI ATGAA 1 cut(s) 492
TspGWI ACGGA 1 cut(s) 770
TspRI CASTG 3 cut(s) 110, 247, 1099
Tth111I GACNNNGTC 1 cut(s) 242
XceI RCATGY 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 650
XspI CTAG 2 cut(s) 15, 687
ZraI GACGTC 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.