Rmu_ssc0000076.1_g000006

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000076.1
OG0013199
Physical Location & Seq
Forward (+)
30400 .. 34216
3817 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1584 bp
atggatggtgctggggagtcttctgattctgggtctgcagtgtctagtgtgaaacctataggaaatgggttttctggtgaagatgataggtgccccaagcaagtgtcgcctattagaggcagtgggtcgaggaatacaagccctttgggttgtgtaaggtcaagaaacactagcccttctaggcaaaaggtggttaagacaaagccgagaggattagatgaggaaacggctgccacatttggtaaagcggttcaccctgatgttcaaatggaggataatatatgggcaatgctgcctgaggacttgttgaatgagatattagctagggttccgccatttatgatattcaggctccgatgtgtttgtaagaggtggaatgcgattctacaagataaaagctttattaagttccactctcaagtgccatctcatgggccttgtcttctcacattttggaagaattctcagaccccgcaatgctcggtgttcagcttgcctctgaaaacatggtacagaataccattcacatttttaccacagtgggcgttctggttggttggctcatctgccggtctagtttgcttctcggggcttgatggcttagtttttaaaaccctagtgtgtaaccctctcacacaaacttggaggactttgcctagtatgcattataatcagcaaaggcagctgataatggttgttgataggaaggatcgctcatttaaagttatagccactagtgatatctatggtgacaaatccttgcccactgaagtatatgattcaaaactcaatagttggtcaattcaccagataatgcccgcagttaatttatgctcctcaaagatggcatattgtgactcaagattgtatttggaaactctttctccgcttggtttgatgatgtatcggctggacacaggttactgggaacacattccagctaagttcccccggtcgttattggatggttatttggttgctggcacccagaagcgtctgtttctagttggaagaatcgacgctaatctcggaacaatgagggctgccaaggctaaggcgggagcaagagccctgaaacctgtcgatgatgggagagttgggaaacgaaaggctgctgctgctccagttgataagagtagcaaaggaaaggccaagcagacaaagaaggccaagaaagaccccaacaagcctaagaggccacctagtgcctttttcgtgttccttggtcagttcagggaagagttcaagaaaaagaaccctaatgtgaaaggggtagcagctgttggaaaagctggaggagaaaagtggagatccctgtctgatgctgaaaaagctccatatgaagctgagtctgcaaaagcaaagactgagtatgaaaagcagctgaaaatctatgacaaaaagcagagtgcacctgatgatggtgatgaagagtcagagaggtcaagatctgaagtgcatgatgaagacgatgaggctagtggggaggaggcgcagccaaaagcggaggaagcagaggaggaagaggtggatgacgaagatgaaggggaggacgagggtgaagaagaggaagatgacgactga

Protein Analysis

527

Amino Acids

58.77

Weight (kDa)

7.04

Isoelectric Point (pI)

50.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 95 - 133 1.1e-06 F-box-like
F-box PF00646 96 - 135 1.1e-09 F-box domain
HMG_box_2 PF09011 394 - 465 4.1e-14 HMG-box domain
HMG_box PF00505 397 - 465 2.1e-21 HMG (high mobility group) box
HMG_WDHD1 PF24815 400 - 447 2.6e-10 WDHD1 HMG box
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013199)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15710 AT5G15710
fragaria_vesca FvH4_6g46160
malus_domestica MD09G1076600.v1.1
prunus_persica Prupe.3G247500_v2.0.a1 Prupe.3G247500_v2.0.a1
pyrus_communis pycom09g00380 pycom17g06740
rosa_chinensis RchiOBHm_Chr2g0164611
rosa_laevigata RLG00000021469
rosa_multiflora Rmu_ssc0000076.1_g000006
rosa_roxburghii Rroxscaffold_2G00086460
rosa_rugosa Rorug02G0509400
rosa_samantha Rh2AG577000 Rh2BG588100 Rh2CG558900 Rh2DG598700
rosa_wichuraiana Rw2G047910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AccB1I GGYRCC 2 cut(s) 90, 983
AccB7I CCANNNNNTGG 1 cut(s) 431
AciI CCGC 7 cut(s) 248, 332, 473, 819, 887, 1058, 1505
AclWI GGATC 2 cut(s) 717, 1305
AcsI RAATTY 1 cut(s) 460
AcuI CTGAAG 2 cut(s) 789, 1473
AdeI CACNNNGTG 1 cut(s) 1205
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 3 cut(s) 116, 431, 1421
AgsI TTSAA 4 cut(s) 266, 310, 783, 1246
AhlI ACTAGT 1 cut(s) 734
Alw21I GWGCWC 1 cut(s) 1414
Alw44I GTGCAC 1 cut(s) 1410
AlwI GGATC 2 cut(s) 717, 1305
Ama87I CYCGRG 1 cut(s) 586
AoxI GGCC 4 cut(s) 434, 1149, 1167, 1196
ApaLI GTGCAC 1 cut(s) 1410
ApoI RAATTY 1 cut(s) 460
Asp700I GAANNNNTTC 1 cut(s) 933
AspLEI GCGC 1 cut(s) 1495
AspS9I GGNCC 1 cut(s) 434
AsuC2I CCSGG 1 cut(s) 952
AsuHPI GGTGA 6 cut(s) 89, 245, 761, 797, 1436, 1571
AvaI CYCGRG 1 cut(s) 586
AxyI CCTNAGG 1 cut(s) 297
BaeGI GKGCMC 2 cut(s) 95, 1414
BanI GGYRCC 2 cut(s) 90, 983
BanII GRGCYC 1 cut(s) 1072
BbsI GAAGAC 3 cut(s) 12, 434, 1473
Bbv12I GWGCWC 1 cut(s) 1414
BccI CCATC 6 cut(s) 433, 590, 838, 959, 1082, 1415
BceAI ACGGC 1 cut(s) 243
BcnI CCSGG 1 cut(s) 952
BcuI ACTAGT 1 cut(s) 734
BfmI CTRYAG 2 cut(s) 36, 57
BglI GCCNNNNNGGC 1 cut(s) 1195
BglII AGATCT 1 cut(s) 1448
Bme1390I CCNGG 1 cut(s) 952
BmeT110I CYCGRG 1 cut(s) 586
BmgT120I GGNCC 1 cut(s) 434
BmiI GGNNCC 4 cut(s) 92, 330, 353, 985
BmrFI CCNGG 1 cut(s) 952
BmrI ACTGGG 1 cut(s) 934
BmsI GCATC 1 cut(s) 1312
BmuI ACTGGG 1 cut(s) 934
BpiI GAAGAC 3 cut(s) 12, 434, 1473
BpmI CTGGAG 2 cut(s) 1107, 1314
Bpu10I CCTNAGC 1 cut(s) 1053
BpuEI CTTGAG 2 cut(s) 402, 844
BpuMI CCSGG 1 cut(s) 952
BsaJI CCNNGG 3 cut(s) 950, 1047, 1222
BsaXI ACNNNNNCTCC 2 cut(s) 868, 898
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 431, 1421
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 2 cut(s) 929, 1124
Bse21I CCTNAGG 1 cut(s) 297
Bse3DI GCAATG 2 cut(s) 294, 482
BseDI CCNNGG 3 cut(s) 950, 1047, 1222
BseGI GGATG 3 cut(s) 10, 970, 1537
BseLI CCNNNNNNNGG 3 cut(s) 116, 431, 1421
BseMI GCAATG 2 cut(s) 294, 482
BseMII CTCAG 4 cut(s) 288, 479, 1338, 1359
BseNI ACTGG 2 cut(s) 929, 1124
BseRI GAGGAG 4 cut(s) 826, 1311, 1502, 1532
BseSI GKGCMC 2 cut(s) 95, 1414
BseYI CCCAGC 1 cut(s) 11
Bsh1285I CGRYCG 1 cut(s) 956
BshFI GGCC 4 cut(s) 436, 1151, 1169, 1198
BshNI GGYRCC 2 cut(s) 90, 983
BsiEI CGRYCG 1 cut(s) 956
BsiHKAI GWGCWC 1 cut(s) 1414
BsiHKCI CYCGRG 1 cut(s) 586
BsiSI CCGG 2 cut(s) 570, 952
BslI CCNNNNNNNGG 3 cut(s) 116, 431, 1421
BsmI GAATGC 1 cut(s) 382
BsnI GGCC 4 cut(s) 436, 1151, 1169, 1198
BsoBI CYCGRG 1 cut(s) 586
Bsp1286I GDGCHC 3 cut(s) 95, 1072, 1414
Bsp143I GATC 3 cut(s) 709, 1310, 1448
BspACI CCGC 7 cut(s) 248, 332, 473, 819, 887, 1058, 1505
BspANI GGCC 4 cut(s) 436, 1151, 1169, 1198
BspCNI CTCAG 4 cut(s) 289, 478, 1339, 1360
BspLI GGNNCC 4 cut(s) 92, 330, 353, 985
BspMAI CTGCAG 1 cut(s) 40
BspPI GGATC 2 cut(s) 717, 1305
BspT107I GGYRCC 2 cut(s) 90, 983
BsrDI GCAATG 2 cut(s) 294, 482
BsrFI RCCGGY 1 cut(s) 569
BsrI ACTGG 2 cut(s) 929, 1124
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 3 cut(s) 950, 1047, 1222
BssMI GATC 3 cut(s) 709, 1310, 1448
BssT1I CCWWGG 2 cut(s) 1047, 1222
Bst4CI ACNGT 1 cut(s) 540
Bst6I CTCTTC 4 cut(s) 1233, 1425, 1518, 1560
BstC8I GCNNGC 3 cut(s) 494, 819, 982
BstDEI CTNAG 8 cut(s) 297, 465, 601, 942, 1053, 1191, 1347, 1368
BstENI CCTNNNNNAGG 1 cut(s) 114
BstF5I GGATG 3 cut(s) 10, 970, 1537
BstHHI GCGC 1 cut(s) 1495
BstKTI GATC 3 cut(s) 712, 1313, 1451
BstMBI GATC 3 cut(s) 709, 1310, 1448
BstMCI CGRYCG 1 cut(s) 956
BstMWI GCNNNNNNNGC 9 cut(s) 106, 661, 682, 1049, 1118, 1195, 1331, 1352, 1511
BstSCI CCNGG 1 cut(s) 950
BstSFI CTRYAG 2 cut(s) 36, 57
BstSLI GKGCMC 2 cut(s) 95, 1414
BstV2I GAAGAC 3 cut(s) 12, 434, 1473
BstX2I RGATCY 2 cut(s) 1310, 1448
BstYI RGATCY 2 cut(s) 1310, 1448
Bsu36I CCTNAGG 1 cut(s) 297
BsuRI GGCC 4 cut(s) 436, 1151, 1169, 1198
BtsCI GGATG 3 cut(s) 10, 970, 1537
BtsI GCAGTG 2 cut(s) 45, 127
BtsIMutI CAGTG 4 cut(s) 45, 127, 545, 765
Cac8I GCNNGC 3 cut(s) 494, 819, 982
CfoI GCGC 1 cut(s) 1495
Cfr10I RCCGGY 1 cut(s) 569
Cfr13I GGNCC 1 cut(s) 434
CseI GACGC 2 cut(s) 983, 1028
Csp6I GTAC 1 cut(s) 511
CviAII CATG 3 cut(s) 431, 507, 1460
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 8 cut(s) 297, 465, 601, 942, 1053, 1191, 1347, 1368
DpnI GATC 3 cut(s) 711, 1312, 1450
DpnII GATC 3 cut(s) 709, 1310, 1448
DraI TTTAAA 2 cut(s) 610, 721
DraIII CACNNNGTG 1 cut(s) 1205
Eam1104I CTCTTC 4 cut(s) 1233, 1425, 1518, 1560
EarI CTCTTC 4 cut(s) 1233, 1425, 1518, 1560
EciI GGCGGA 1 cut(s) 321
Eco130I CCWWGG 2 cut(s) 1047, 1222
Eco24I GRGCYC 1 cut(s) 1072
Eco32I GATATC 1 cut(s) 742
Eco57I CTGAAG 2 cut(s) 789, 1473
Eco81I CCTNAGG 1 cut(s) 297
Eco88I CYCGRG 1 cut(s) 586
EcoNI CCTNNNNNAGG 1 cut(s) 114
EcoRI GAATTC 1 cut(s) 460
EcoRV GATATC 1 cut(s) 742
EcoT14I CCWWGG 2 cut(s) 1047, 1222
EcoT22I ATGCAT 1 cut(s) 666
EcoT38I GRGCYC 1 cut(s) 1072
ErhI CCWWGG 2 cut(s) 1047, 1222
FaeI CATG 3 cut(s) 434, 510, 1463
FatI CATG 3 cut(s) 430, 506, 1459
FauI CCCGC 3 cut(s) 480, 826, 1051
FauNDI CATATG 1 cut(s) 1339
FokI GGATG 3 cut(s) 17, 977, 1544
FriOI GRGCYC 1 cut(s) 1072
GlaI GCGC 1 cut(s) 1494
GsaI CCCAGC 1 cut(s) 15
GsuI CTGGAG 2 cut(s) 1107, 1314
HaeIII GGCC 4 cut(s) 436, 1151, 1169, 1198
HapII CCGG 2 cut(s) 570, 952
HgaI GACGC 2 cut(s) 983, 1028
HhaI GCGC 1 cut(s) 1495
Hin1II CATG 3 cut(s) 434, 510, 1463
Hin6I GCGC 1 cut(s) 1493
HinP1I GCGC 1 cut(s) 1493
HindIII AAGCTT 1 cut(s) 397
HinfI GANTC 8 cut(s) 17, 26, 382, 779, 857, 1014, 1349, 1433
HpaII CCGG 2 cut(s) 570, 952
HphI GGTGA 6 cut(s) 89, 245, 761, 797, 1436, 1571
Hpy166II GTNNAC 2 cut(s) 253, 1412
Hpy188I TCNGA 8 cut(s) 25, 356, 468, 501, 1031, 1321, 1438, 1453
Hpy188III TCNNGA 4 cut(s) 162, 861, 1246, 1446
Hpy8I GTNNAC 2 cut(s) 253, 1412
Hpy99I CGWCG 1 cut(s) 1022
HpyAV CCTTC 4 cut(s) 186, 700, 1159, 1538
HpyCH4III ACNGT 1 cut(s) 540
HpyCH4V TGCA 5 cut(s) 38, 664, 1355, 1412, 1459
HpyF10VI GCNNNNNNNGC 9 cut(s) 106, 661, 682, 1049, 1118, 1195, 1331, 1352, 1511
HpyF3I CTNAG 8 cut(s) 297, 465, 601, 942, 1053, 1191, 1347, 1368
Hsp92II CATG 3 cut(s) 434, 510, 1463
HspAI GCGC 1 cut(s) 1493
Kzo9I GATC 3 cut(s) 709, 1310, 1448
LmnI GCTCC 5 cut(s) 357, 839, 1061, 1126, 1339
LweI GCATC 1 cut(s) 1312
MaeIII GTNAC 4 cut(s) 623, 749, 854, 920
MalI GATC 3 cut(s) 711, 1312, 1450
MboI GATC 3 cut(s) 709, 1310, 1448
MflI RGATCY 2 cut(s) 1310, 1448
MhlI GDGCHC 3 cut(s) 95, 1072, 1414
MluCI AATT 3 cut(s) 460, 801, 826
MlyI GAGTC 4 cut(s) 26, 851, 1358, 1442
MmeI TCCRAC 2 cut(s) 988, 1264
Mph1103I ATGCAT 1 cut(s) 666
MroXI GAANNNNTTC 1 cut(s) 933
MseI TTAA 5 cut(s) 195, 405, 609, 720, 825
MslI CAYNNNNRTG 1 cut(s) 258
MspA1I CMGCKG 3 cut(s) 685, 1280, 1384
MspI CCGG 2 cut(s) 570, 952
MspR9I CCNGG 1 cut(s) 952
Mva1269I GAATGC 1 cut(s) 382
MwoI GCNNNNNNNGC 9 cut(s) 106, 661, 682, 1049, 1118, 1195, 1331, 1352, 1511
NciI CCSGG 1 cut(s) 952
NdeI CATATG 1 cut(s) 1339
NdeII GATC 3 cut(s) 709, 1310, 1448
NlaIII CATG 3 cut(s) 434, 510, 1463
NlaIV GGNNCC 4 cut(s) 92, 330, 353, 985
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 2 cut(s) 749, 854
NsiI ATGCAT 1 cut(s) 666
PctI GAATGC 1 cut(s) 382
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 4 cut(s) 26, 382, 779, 1014
PflMI CCANNNNNTGG 1 cut(s) 431
PleI GAGTC 4 cut(s) 25, 851, 1357, 1441
PpsI GAGTC 4 cut(s) 25, 851, 1357, 1441
PsiI TTATAA 1 cut(s) 669
PspFI CCCAGC 1 cut(s) 11
PspN4I GGNNCC 4 cut(s) 92, 330, 353, 985
PspPI GGNCC 1 cut(s) 434
PstI CTGCAG 1 cut(s) 40
PsuI RGATCY 2 cut(s) 1310, 1448
PvuII CAGCTG 3 cut(s) 685, 1280, 1384
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
RseI CAYNNNNRTG 1 cut(s) 258
SaqAI TTAA 5 cut(s) 195, 405, 609, 720, 825
Sau3AI GATC 3 cut(s) 709, 1310, 1448
Sau96I GGNCC 1 cut(s) 434
SchI GAGTC 4 cut(s) 26, 851, 1358, 1442
ScrFI CCNGG 1 cut(s) 952
SduI GDGCHC 3 cut(s) 95, 1072, 1414
SfaNI GCATC 1 cut(s) 1312
SfcI CTRYAG 2 cut(s) 36, 57
SmiMI CAYNNNNRTG 1 cut(s) 258
SmlI CTYRAG 2 cut(s) 417, 859
SmoI CTYRAG 2 cut(s) 417, 859
SpeI ACTAGT 1 cut(s) 734
Sse9I AATT 3 cut(s) 460, 801, 826
SsiI CCGC 7 cut(s) 248, 332, 473, 819, 887, 1058, 1505
StyD4I CCNGG 1 cut(s) 950
StyI CCWWGG 2 cut(s) 1047, 1222
TaaI ACNGT 1 cut(s) 540
TaqI TCGA 3 cut(s) 128, 1017, 1083
TasI AATT 3 cut(s) 460, 801, 826
TfiI GAWTC 4 cut(s) 26, 382, 779, 1014
Tru1I TTAA 5 cut(s) 195, 405, 609, 720, 825
Tru9I TTAA 5 cut(s) 195, 405, 609, 720, 825
TscAI CASTG 4 cut(s) 45, 127, 545, 772
TseFI GTSAC 2 cut(s) 749, 854
Tsp45I GTSAC 2 cut(s) 749, 854
TspDTI ATGAA 5 cut(s) 1356, 1389, 1443, 1479, 1557
TspRI CASTG 4 cut(s) 45, 127, 545, 772
Van91I CCANNNNNTGG 1 cut(s) 431
VneI GTGCAC 1 cut(s) 1410
XagI CCTNNNNNAGG 1 cut(s) 114
XapI RAATTY 1 cut(s) 460
XmnI GAANNNNTTC 1 cut(s) 933
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.