Rmu_ssc0000158.1_g000005

Aspartate aminotransferase

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000158.1
Physical Location & Seq
Forward (+)
23716 .. 26864
3149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000158.1_g000005.1.cds

Sequence Viewer

Length: 1368 bp
atggcttcaaccatgctgtccttggcttctgtctctccgtcatcaatccatgacactcacaagggcaagatcaagcttggcagtttggagagaagcaatctatttctaaagaccgagtctcttggtcgggtatctatggttgctgctgtcaatacttctcgatttgacggtgtgaccatggccccacctgatcctattcttggagtttctgaagcattcaaagctagcacaaatgacaagaagctcaaccttggagttggggcataccgcactgaagaactccacccatatgtgcttactgttgttaagaaggcagagaatcttatgcttcaaaggggggaaaacaaagagtacctcccgattgaaggtttggcagcattcaacaaagcaactgcagagttattatttggagcagacaacccagtaataaaggagcaaagggtcgcaactgttcaaggcctgtcaggaactggttctcttcgtcttgctgcagcttttatagaacgatattttcctggagccaaagctttaatatcatctccgacctggggtaatcacaagaatatttttaatgacgctggagttccctggtctgagtaccgatactatgatccaaaaacagttggtttggactttgaggggatgatagcggacataaaggcagcacctgaaggatcctttgtgttgcttcatggctgtgctcacaatcctactggcatagatccaacccctgatcagtgggaaaaaattgctgatgtcattcaagagaaaaaccatatccccttttttgatgttgcataccagggatttgctagtggaagcttggatgctgatgctgcatctgtcagattgtttgccgcacgtggtatggagctcctggttgctcagtcgtacagtaaaaaccttggtctctatgctgaaagaattggagcaattaatgttgtcttgacctcatctgatacagcagtaagggtaaagagtcaactgaagaggcttgctcgacctatgtactcaaacccgcctattcacggggctaagatcgtggccaatattgttggggatccgacacttttcagtgagtggaaagcagagatggaaatgatggctggaagaataaagaatgtgagacaaaaactatacgatagcctgtgcgcaaaagataagagtggaaaggattggtcatttatacttaagcagattggcatgttttcattcacaggcttgaacaaaactcagactgagaatatgacaaacaagtggcatgtattcatgacaaaggacggaagaatatccctggctggattgaatttggcgaaatgtgaatacctttcagatgccatcattgattcgtaccacaatgtgagttga

Protein Analysis

455

Amino Acids

49.82

Weight (kDa)

8.67

Isoelectric Point (pI)

30.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1153
AciI CCGC 4 cut(s) 268, 650, 858, 1019
AclWI GGATC 7 cut(s) 185, 605, 669, 682, 716, 1055, 1068
AcoI YGGCCR 1 cut(s) 1044
AcsI RAATTY 1 cut(s) 1306
AcuI CTGAAG 4 cut(s) 231, 294, 690, 1007
AcvI CACGTG 1 cut(s) 863
AdeI CACNNNGTG 1 cut(s) 1360
AfaI GTAC 5 cut(s) 353, 599, 893, 1010, 1352
AfiI CCNNNNNNNGG 4 cut(s) 200, 365, 548, 1028
AflII CTTAAG 1 cut(s) 1190
AgsI TTSAA 9 cut(s) 9, 220, 332, 365, 382, 455, 764, 1225, 1306
AjnI CCWGG 6 cut(s) 514, 545, 587, 801, 876, 1293
AluBI AGCT 7 cut(s) 76, 224, 244, 494, 527, 822, 874
AluI AGCT 7 cut(s) 76, 224, 244, 494, 527, 822, 874
Alw21I GWGCWC 2 cut(s) 703, 876
Alw26I GTCTC 4 cut(s) 37, 123, 914, 1120
AlwI GGATC 7 cut(s) 185, 605, 669, 682, 716, 1055, 1068
AlwNI CAGNNNCTG 2 cut(s) 470, 668
AoxI GGCC 3 cut(s) 180, 457, 1044
ApeKI GCWGC 6 cut(s) 143, 374, 488, 491, 662, 836
ApoI RAATTY 1 cut(s) 1306
AseI ATTAAT 1 cut(s) 936
Asp700I GAANNNNTTC 1 cut(s) 472
AspLEI GCGC 1 cut(s) 1154
AspS9I GGNCC 1 cut(s) 181
AsuNHI GCTAGC 1 cut(s) 224
BaeI ACNNNNGTAYC 2 cut(s) 951, 984
BalI TGGCCA 1 cut(s) 1046
BamHI GGATCC 2 cut(s) 674, 1060
BanII GRGCYC 1 cut(s) 876
BbrPI CACGTG 1 cut(s) 863
Bbv12I GWGCWC 2 cut(s) 703, 876
BbvI GCAGC 6 cut(s) 130, 386, 475, 503, 674, 823
BccI CCATC 3 cut(s) 1087, 1096, 1346
BciT130I CCWGG 6 cut(s) 516, 547, 589, 803, 878, 1295
BclI TGATCA 1 cut(s) 733
BcoDI GTCTC 4 cut(s) 37, 123, 914, 1120
BfaI CTAG 2 cut(s) 225, 813
BfmI CTRYAG 2 cut(s) 393, 489
BfrI CTTAAG 1 cut(s) 1190
BisI GCNGC 7 cut(s) 144, 375, 489, 492, 663, 837, 858
BlsI GCNGC 7 cut(s) 145, 376, 490, 493, 664, 838, 859
Bme1390I CCNGG 6 cut(s) 516, 547, 589, 803, 878, 1295
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 4 cut(s) 183, 520, 676, 1062
BmrFI CCNGG 6 cut(s) 516, 547, 589, 803, 878, 1295
BmrI ACTGGG 1 cut(s) 416
BmsI GCATC 4 cut(s) 817, 823, 848, 1324
BmtI GCTAGC 1 cut(s) 228
BmuI ACTGGG 1 cut(s) 416
BplI GAGNNNNNCTC 2 cut(s) 982, 1014
BpmI CTGGAG 2 cut(s) 537, 600
BsaAI YACGTR 1 cut(s) 863
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 8 cut(s) 21, 177, 250, 546, 587, 802, 904, 1293
Bsc4I CCNNNNNNNGG 4 cut(s) 200, 365, 548, 1028
Bse1I ACTGG 3 cut(s) 422, 475, 718
BseBI CCWGG 6 cut(s) 516, 547, 589, 803, 878, 1295
BseDI CCNNGG 8 cut(s) 21, 177, 250, 546, 587, 802, 904, 1293
BseGI GGATG 2 cut(s) 648, 832
BseLI CCNNNNNNNGG 4 cut(s) 200, 365, 548, 1028
BseMII CTCAG 4 cut(s) 585, 899, 1230, 1247
BseNI ACTGG 3 cut(s) 422, 475, 718
BseXI GCAGC 6 cut(s) 130, 386, 475, 503, 674, 823
BshFI GGCC 3 cut(s) 182, 459, 1046
BsiHKAI GWGCWC 2 cut(s) 703, 876
BslI CCNNNNNNNGG 4 cut(s) 200, 365, 548, 1028
BsmAI GTCTC 4 cut(s) 37, 123, 914, 1120
BsmI GAATGC 2 cut(s) 215, 377
BsnI GGCC 3 cut(s) 182, 459, 1046
Bso31I GGTCTC 1 cut(s) 914
Bsp1286I GDGCHC 2 cut(s) 703, 876
Bsp143I GATC 8 cut(s) 69, 190, 610, 674, 721, 733, 1038, 1060
Bsp19I CCATGG 1 cut(s) 177
BspACI CCGC 4 cut(s) 268, 650, 858, 1019
BspANI GGCC 3 cut(s) 182, 459, 1046
BspCNI CTCAG 4 cut(s) 586, 898, 1231, 1246
BspHI TCATGA 1 cut(s) 1269
BspLI GGNNCC 4 cut(s) 183, 520, 676, 1062
BspMAI CTGCAG 2 cut(s) 397, 493
BspOI GCTAGC 1 cut(s) 228
BspPI GGATC 7 cut(s) 185, 605, 669, 682, 716, 1055, 1068
BspTI CTTAAG 1 cut(s) 1190
BspTNI GGTCTC 1 cut(s) 914
BsrI ACTGG 3 cut(s) 422, 475, 718
BssECI CCNNGG 8 cut(s) 21, 177, 250, 546, 587, 802, 904, 1293
BssMI GATC 8 cut(s) 69, 190, 610, 674, 721, 733, 1038, 1060
BssT1I CCWWGG 4 cut(s) 21, 177, 250, 904
Bst2UI CCWGG 6 cut(s) 516, 547, 589, 803, 878, 1295
Bst4CI ACNGT 5 cut(s) 170, 301, 451, 622, 896
Bst6I CTCTTC 2 cut(s) 483, 983
BstAFI CTTAAG 1 cut(s) 1190
BstBAI YACGTR 1 cut(s) 863
BstC8I GCNNGC 2 cut(s) 226, 996
BstDEI CTNAG 5 cut(s) 594, 885, 1035, 1233, 1239
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 2 cut(s) 648, 832
BstHHI GCGC 1 cut(s) 1154
BstKTI GATC 8 cut(s) 72, 193, 613, 677, 724, 736, 1041, 1063
BstMAI GTCTC 4 cut(s) 37, 123, 914, 1120
BstMBI GATC 8 cut(s) 69, 190, 610, 674, 721, 733, 1038, 1060
BstMWI GCNNNNNNNGC 2 cut(s) 221, 836
BstNI CCWGG 6 cut(s) 516, 547, 589, 803, 878, 1295
BstNSI RCATGY 2 cut(s) 1207, 1265
BstSCI CCNGG 6 cut(s) 514, 545, 587, 801, 876, 1293
BstSFI CTRYAG 2 cut(s) 393, 489
BstV1I GCAGC 6 cut(s) 130, 386, 475, 503, 674, 823
BstX2I RGATCY 3 cut(s) 674, 721, 1060
BstYI RGATCY 3 cut(s) 674, 721, 1060
BsuRI GGCC 3 cut(s) 182, 459, 1046
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 2 cut(s) 648, 832
BtsIMutI CAGTG 3 cut(s) 270, 743, 1081
Cac8I GCNNGC 2 cut(s) 226, 996
CaiI CAGNNNCTG 2 cut(s) 470, 668
CciI TCATGA 1 cut(s) 1269
CfoI GCGC 1 cut(s) 1154
Cfr13I GGNCC 1 cut(s) 181
CseI GACGC 1 cut(s) 584
Csp6I GTAC 5 cut(s) 352, 598, 892, 1009, 1351
CviAII CATG 7 cut(s) 13, 50, 178, 692, 1204, 1262, 1270
CviQI GTAC 5 cut(s) 352, 598, 892, 1009, 1351
DdeI CTNAG 5 cut(s) 594, 885, 1035, 1233, 1239
DpnI GATC 8 cut(s) 71, 192, 612, 676, 723, 735, 1040, 1062
DpnII GATC 8 cut(s) 69, 190, 610, 674, 721, 733, 1038, 1060
DraIII CACNNNGTG 1 cut(s) 1360
EaeI YGGCCR 1 cut(s) 1044
Eam1104I CTCTTC 2 cut(s) 483, 983
EarI CTCTTC 2 cut(s) 483, 983
Ecl136II GAGCTC 1 cut(s) 874
Eco130I CCWWGG 4 cut(s) 21, 177, 250, 904
Eco147I AGGCCT 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 876
Eco31I GGTCTC 1 cut(s) 914
Eco53kI GAGCTC 1 cut(s) 874
Eco57I CTGAAG 4 cut(s) 231, 294, 690, 1007
Eco72I CACGTG 1 cut(s) 863
EcoICRI GAGCTC 1 cut(s) 874
EcoRII CCWGG 6 cut(s) 514, 545, 587, 801, 876, 1293
EcoT14I CCWWGG 4 cut(s) 21, 177, 250, 904
EcoT38I GRGCYC 1 cut(s) 876
ErhI CCWWGG 4 cut(s) 21, 177, 250, 904
FaeI CATG 7 cut(s) 16, 53, 181, 695, 1207, 1265, 1273
FatI CATG 7 cut(s) 12, 49, 177, 691, 1203, 1261, 1269
FauI CCCGC 1 cut(s) 1026
FauNDI CATATG 1 cut(s) 289
FbaI TGATCA 1 cut(s) 733
Fnu4HI GCNGC 7 cut(s) 144, 375, 489, 492, 663, 837, 858
FokI GGATG 2 cut(s) 655, 839
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 7 cut(s) 144, 375, 489, 492, 663, 837, 858
FspBI CTAG 2 cut(s) 225, 813
FspI TGCGCA 1 cut(s) 1153
GlaI GCGC 1 cut(s) 1153
GluI GCNGC 7 cut(s) 144, 375, 489, 492, 663, 837, 858
GsuI CTGGAG 2 cut(s) 537, 600
HaeIII GGCC 3 cut(s) 182, 459, 1046
HgaI GACGC 1 cut(s) 584
HhaI GCGC 1 cut(s) 1154
Hin1II CATG 7 cut(s) 16, 53, 181, 695, 1207, 1265, 1273
Hin6I GCGC 1 cut(s) 1152
HinP1I GCGC 1 cut(s) 1152
HincII GTYRAC 1 cut(s) 983
HindII GTYRAC 1 cut(s) 983
HindIII AAGCTT 3 cut(s) 74, 525, 820
HinfI GANTC 4 cut(s) 116, 319, 979, 1346
Hpy166II GTNNAC 1 cut(s) 983
Hpy188I TCNGA 8 cut(s) 211, 543, 595, 848, 958, 1065, 1236, 1333
Hpy188III TCNNGA 6 cut(s) 159, 358, 465, 764, 946, 1270
Hpy8I GTNNAC 1 cut(s) 983
HpyAV CCTTC 3 cut(s) 304, 359, 665
HpyCH4III ACNGT 5 cut(s) 170, 301, 451, 622, 896
HpyCH4IV ACGT 1 cut(s) 862
HpyCH4V TGCA 4 cut(s) 395, 491, 797, 839
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 836
HpyF3I CTNAG 5 cut(s) 594, 885, 1035, 1233, 1239
HpySE526I ACGT 1 cut(s) 862
Hsp92II CATG 7 cut(s) 16, 53, 181, 695, 1207, 1265, 1273
HspAI GCGC 1 cut(s) 1152
Ksp22I TGATCA 1 cut(s) 733
Kzo9I GATC 8 cut(s) 69, 190, 610, 674, 721, 733, 1038, 1060
LmnI GCTCC 6 cut(s) 410, 433, 518, 871, 879, 929
Lsp1109I GCAGC 6 cut(s) 130, 386, 475, 503, 674, 823
LweI GCATC 4 cut(s) 817, 823, 848, 1324
MaeI CTAG 2 cut(s) 225, 813
MaeII ACGT 1 cut(s) 862
MaeIII GTNAC 1 cut(s) 172
MalI GATC 8 cut(s) 71, 192, 612, 676, 723, 735, 1040, 1062
MboI GATC 8 cut(s) 69, 190, 610, 674, 721, 733, 1038, 1060
MboII GAAGA 5 cut(s) 287, 470, 1000, 1122, 1296
MflI RGATCY 3 cut(s) 674, 721, 1060
MhlI GDGCHC 2 cut(s) 703, 876
MlsI TGGCCA 1 cut(s) 1046
MluCI AATT 4 cut(s) 747, 924, 933, 1306
MluNI TGGCCA 1 cut(s) 1046
MlyI GAGTC 2 cut(s) 125, 988
MmeI TCCRAC 3 cut(s) 566, 749, 1088
MnlI CCTC 4 cut(s) 365, 631, 961, 984
Mox20I TGGCCA 1 cut(s) 1046
MroXI GAANNNNTTC 1 cut(s) 472
MscI TGGCCA 1 cut(s) 1046
MseI TTAA 5 cut(s) 306, 530, 570, 936, 1191
MslI CAYNNNNRTG 2 cut(s) 288, 696
Msp20I TGGCCA 1 cut(s) 1046
MspCI CTTAAG 1 cut(s) 1190
MspR9I CCNGG 6 cut(s) 516, 547, 589, 803, 878, 1295
Mva1269I GAATGC 2 cut(s) 215, 377
MvaI CCWGG 6 cut(s) 516, 547, 589, 803, 878, 1295
MwoI GCNNNNNNNGC 2 cut(s) 221, 836
NcoI CCATGG 1 cut(s) 177
NdeI CATATG 1 cut(s) 289
NdeII GATC 8 cut(s) 69, 190, 610, 674, 721, 733, 1038, 1060
NheI GCTAGC 1 cut(s) 224
NlaIII CATG 7 cut(s) 16, 53, 181, 695, 1207, 1265, 1273
NlaIV GGNNCC 4 cut(s) 183, 520, 676, 1062
NmuCI GTSAC 1 cut(s) 172
NsbI TGCGCA 1 cut(s) 1153
NspI RCATGY 2 cut(s) 1207, 1265
PagI TCATGA 1 cut(s) 1269
PceI AGGCCT 1 cut(s) 459
PctI GAATGC 2 cut(s) 215, 377
PdmI GAANNNNTTC 1 cut(s) 472
PfeI GAWTC 2 cut(s) 319, 1346
PflFI GACNNNGTC 1 cut(s) 115
PfoI TCCNGGA 1 cut(s) 514
PkrI GCNGC 7 cut(s) 145, 376, 490, 493, 664, 838, 859
PleI GAGTC 2 cut(s) 124, 987
PmaCI CACGTG 1 cut(s) 863
PmlI CACGTG 1 cut(s) 863
PpsI GAGTC 2 cut(s) 124, 987
Ppu21I YACGTR 1 cut(s) 863
PshBI ATTAAT 1 cut(s) 936
Psp124BI GAGCTC 1 cut(s) 876
Psp6I CCWGG 6 cut(s) 514, 545, 587, 801, 876, 1293
PspCI CACGTG 1 cut(s) 863
PspGI CCWGG 6 cut(s) 514, 545, 587, 801, 876, 1293
PspN4I GGNNCC 4 cut(s) 183, 520, 676, 1062
PspPI GGNCC 1 cut(s) 181
PstI CTGCAG 2 cut(s) 397, 493
PstNI CAGNNNCTG 2 cut(s) 470, 668
PsuI RGATCY 3 cut(s) 674, 721, 1060
PsyI GACNNNGTC 1 cut(s) 115
RsaI GTAC 5 cut(s) 353, 599, 893, 1010, 1352
RsaNI GTAC 5 cut(s) 352, 598, 892, 1009, 1351
RseI CAYNNNNRTG 2 cut(s) 288, 696
SacI GAGCTC 1 cut(s) 876
SaqAI TTAA 5 cut(s) 306, 530, 570, 936, 1191
SatI GCNGC 7 cut(s) 144, 375, 489, 492, 663, 837, 858
Sau3AI GATC 8 cut(s) 69, 190, 610, 674, 721, 733, 1038, 1060
Sau96I GGNCC 1 cut(s) 181
SchI GAGTC 2 cut(s) 125, 988
ScrFI CCNGG 6 cut(s) 516, 547, 589, 803, 878, 1295
SduI GDGCHC 2 cut(s) 703, 876
SfaNI GCATC 4 cut(s) 817, 823, 848, 1324
SfcI CTRYAG 2 cut(s) 393, 489
SmiMI CAYNNNNRTG 2 cut(s) 288, 696
SmlI CTYRAG 1 cut(s) 1190
SmoI CTYRAG 1 cut(s) 1190
Sse9I AATT 4 cut(s) 747, 924, 933, 1306
SseBI AGGCCT 1 cut(s) 459
SsiI CCGC 4 cut(s) 268, 650, 858, 1019
SspI AATATT 2 cut(s) 565, 1051
SspMI CTAG 2 cut(s) 225, 813
SstI GAGCTC 1 cut(s) 876
StuI AGGCCT 1 cut(s) 459
StyD4I CCNGG 6 cut(s) 514, 545, 587, 801, 876, 1293
StyI CCWWGG 4 cut(s) 21, 177, 250, 904
TaaI ACNGT 5 cut(s) 170, 301, 451, 622, 896
TaiI ACGT 1 cut(s) 865
TaqI TCGA 2 cut(s) 160, 1000
TaqII GACCGA 1 cut(s) 128
TasI AATT 4 cut(s) 747, 924, 933, 1306
TatI WGTACW 1 cut(s) 1008
TauI GCSGC 1 cut(s) 860
TfiI GAWTC 2 cut(s) 319, 1346
Tru1I TTAA 5 cut(s) 306, 530, 570, 936, 1191
Tru9I TTAA 5 cut(s) 306, 530, 570, 936, 1191
TscAI CASTG 3 cut(s) 277, 743, 1081
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 6 cut(s) 143, 374, 488, 491, 662, 836
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 3 cut(s) 680, 1200, 1258
TspGWI ACGGA 2 cut(s) 27, 1296
TspRI CASTG 3 cut(s) 277, 743, 1081
Tth111I GACNNNGTC 1 cut(s) 115
Vha464I CTTAAG 1 cut(s) 1190
VspI ATTAAT 1 cut(s) 936
XapI RAATTY 1 cut(s) 1306
XceI RCATGY 2 cut(s) 1207, 1265
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 472
XspI CTAG 2 cut(s) 225, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.