Rorug06G0292500

Aspartate aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007966
Physical Location & Seq
Reverse (-)
44460676 .. 44463655
2980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0292500.1

Sequence Viewer

Length: 933 bp
ATGCAGGGTTTTTCTGGAGGTTTAGGAACTGGGTCTGTGACTAACATGTCGACCGTGTCCAAAGATGAGAACTTTGCTATGTCTTCTGAGGATTCTTGTAGCCCAGATGAGTCTGATCAGCTCGAGCTCTGTCTTGGGTTGAGTCTCGGCGGTGGCGGCAAGGCGGCCCAGCAAGGCCCAGTTGGGAATCATGCTAGGATTCTTACGGCCAAGGATTTCCGTTATGTGGGTTCTAACTCTAAGTCGTCGGCGGCGGCTGCTTCTTCGTCGTCGTCGTCGTCGTCTTCTTCTTCGTTGAGCAGTGCTAATGTTACGGCTGGGACTAAGAGGAGTGCTGATTCTGTGGCGGCGGCTAATGCTGCCAATCAGGTTGTGGGATGGCCTCCTATCAGAGCTTATAGAATGAATTCCTTGGTTCATCAAGCAAAATCTTCAGCCACTGAAGTATTTAACTCGGAGAAAGGTGAATGCAAGATTATTTCGGGGAAGGCTAACATTGGTGGTCAAGAAAACAATGCAAATGCTAAGGAGAGAGGACGCCTCACTAGTTCCCTGTTTGTGAAGGTGAATATGGATGGAAGTCCAATCGGAAGGAAGGTTAACCTAACTGCGCATAGCAGCTATGAAGCCCTAGCACAAATGTTGGAGGATATGTTCCAGGGACCCTCTGCAAATGCGAAATTGATACGTTCTGGTGGTCAGGAGCATGATATGATGGTGGGAGAAACTACACCCTCAAAGTTGCTAGATGGATCATTTGATTTTGCACTCACTTATGAAGACAAAGAAGGTGACTGGATGCTTGTTGGGGATGTTCCTTGGGGGATGTTTCTTTGTTCTGTGAAGAGGCTAAGAATCATGAGGACATCTGAGGCTAATGGACTTGCTCCGAGGCTGCAGGAAAGGAATGCAAGGCAGAGATGCGCGAACTGA

Protein Analysis

310

Amino Acids

32.67

Weight (kDa)

7.63

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 41 - 298 1e-64 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 46
Acc16I TGCGCA 1 cut(s) 612
AccI GTMKAC 1 cut(s) 50
AccII CGCG 1 cut(s) 926
AciI CCGC 7 cut(s) 150, 156, 164, 251, 254, 347, 350
AclWI GGATC 1 cut(s) 760
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 1 cut(s) 406
AcuI CTGAAG 2 cut(s) 417, 462
AcyI GRCGYC 1 cut(s) 538
AfiI CCNNNNNNNGG 1 cut(s) 226
AflIII ACRYGT 1 cut(s) 45
AhlI ACTAGT 1 cut(s) 545
AjnI CCWGG 1 cut(s) 657
AloI GAACNNNNNNTCC 2 cut(s) 638, 670
AluBI AGCT 4 cut(s) 121, 127, 395, 621
AluI AGCT 4 cut(s) 121, 127, 395, 621
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 1 cut(s) 760
AlwNI CAGNNNCTG 1 cut(s) 440
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 4 cut(s) 165, 175, 207, 380
ApeKI GCWGC 4 cut(s) 257, 359, 618, 895
ApoI RAATTY 1 cut(s) 406
Asp700I GAANNNNTTC 1 cut(s) 406
AspLEI GCGC 2 cut(s) 613, 926
AspS9I GGNCC 3 cut(s) 166, 176, 662
AsuHPI GGTGA 3 cut(s) 476, 577, 803
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 1 cut(s) 662
BanII GRGCYC 1 cut(s) 129
BbsI GAAGAC 3 cut(s) 75, 276, 786
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 4 cut(s) 244, 346, 630, 882
BccI CCATC 4 cut(s) 372, 569, 709, 743
BceAI ACGGC 2 cut(s) 222, 330
BciT130I CCWGG 1 cut(s) 659
BclI TGATCA 1 cut(s) 115
BcoDI GTCTC 1 cut(s) 149
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 4 cut(s) 195, 546, 632, 746
BfmI CTRYAG 1 cut(s) 896
Bme1390I CCNGG 1 cut(s) 659
Bme18I GGWCC 1 cut(s) 662
BmeT110I CYCGRG 1 cut(s) 122
BmgT120I GGNCC 3 cut(s) 166, 176, 662
BmiI GGNNCC 2 cut(s) 663, 664
BmrFI CCNGG 1 cut(s) 659
BmrI ACTGGG 2 cut(s) 39, 173
BmsI GCATC 2 cut(s) 789, 911
BmuI ACTGGG 2 cut(s) 39, 173
BpiI GAAGAC 3 cut(s) 75, 276, 786
BplI GAGNNNNNCTC 2 cut(s) 525, 557
BpmI CTGGAG 1 cut(s) 36
Bpu10I CCTNAGC 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 538
BsaJI CCNNGG 5 cut(s) 210, 411, 658, 818, 890
BsaXI ACNNNNNCTCC 2 cut(s) 638, 668
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse1I ACTGG 3 cut(s) 34, 179, 800
BseBI CCWGG 1 cut(s) 659
BseDI CCNNGG 5 cut(s) 210, 411, 658, 818, 890
BseGI GGATG 5 cut(s) 383, 580, 804, 817, 831
BseLI CCNNNNNNNGG 1 cut(s) 226
BseMII CTCAG 2 cut(s) 78, 861
BseNI ACTGG 3 cut(s) 34, 179, 800
BseRI GAGGAG 1 cut(s) 343
BseXI GCAGC 4 cut(s) 244, 346, 630, 882
BseYI CCCAGC 2 cut(s) 168, 317
Bsh1236I CGCG 1 cut(s) 926
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 4 cut(s) 167, 177, 209, 382
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 122
BslFI GGGAC 2 cut(s) 334, 675
BslI CCNNNNNNNGG 1 cut(s) 226
BsmAI GTCTC 1 cut(s) 149
BsmFI GGGAC 2 cut(s) 334, 675
BsmI GAATGC 2 cut(s) 473, 913
BsnI GGCC 4 cut(s) 167, 177, 209, 382
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 2 cut(s) 115, 752
BspACI CCGC 7 cut(s) 150, 156, 164, 251, 254, 347, 350
BspANI GGCC 4 cut(s) 167, 177, 209, 382
BspCNI CTCAG 2 cut(s) 79, 862
BspFNI CGCG 1 cut(s) 926
BspHI TCATGA 1 cut(s) 858
BspLI GGNNCC 2 cut(s) 663, 664
BspMAI CTGCAG 1 cut(s) 900
BspPI GGATC 1 cut(s) 760
BsrI ACTGG 3 cut(s) 34, 179, 800
BssECI CCNNGG 5 cut(s) 210, 411, 658, 818, 890
BssMI GATC 2 cut(s) 115, 752
BssNI GRCGYC 1 cut(s) 538
BssT1I CCWWGG 3 cut(s) 210, 411, 818
Bst2UI CCWGG 1 cut(s) 659
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 1 cut(s) 839
BstACI GRCGYC 1 cut(s) 538
BstDEI CTNAG 6 cut(s) 87, 240, 324, 525, 851, 870
BstF5I GGATG 5 cut(s) 383, 580, 804, 817, 831
BstFNI CGCG 1 cut(s) 926
BstHHI GCGC 2 cut(s) 613, 926
BstKTI GATC 2 cut(s) 118, 755
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 2 cut(s) 115, 752
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 4 cut(s) 156, 257, 356, 359
BstNI CCWGG 1 cut(s) 659
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 657
BstSFI CTRYAG 1 cut(s) 896
BstUI CGCG 1 cut(s) 926
BstV1I GCAGC 4 cut(s) 244, 346, 630, 882
BstV2I GAAGAC 3 cut(s) 75, 276, 786
BsuRI GGCC 4 cut(s) 167, 177, 209, 382
BtsCI GGATG 5 cut(s) 383, 580, 804, 817, 831
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 307, 438
CaiI CAGNNNCTG 1 cut(s) 440
CciI TCATGA 1 cut(s) 858
CfoI GCGC 2 cut(s) 613, 926
Cfr13I GGNCC 3 cut(s) 166, 176, 662
CseI GACGC 1 cut(s) 546
CviAII CATG 4 cut(s) 46, 191, 707, 859
DdeI CTNAG 6 cut(s) 87, 240, 324, 525, 851, 870
DpnI GATC 2 cut(s) 117, 754
DpnII GATC 2 cut(s) 115, 752
DrdI GACNNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 839
EarI CTCTTC 1 cut(s) 839
Ecl136II GAGCTC 1 cut(s) 127
Eco130I CCWWGG 3 cut(s) 210, 411, 818
Eco24I GRGCYC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 662
Eco53kI GAGCTC 1 cut(s) 127
Eco57I CTGAAG 2 cut(s) 417, 462
Eco88I CYCGRG 1 cut(s) 122
EcoICRI GAGCTC 1 cut(s) 127
EcoO109I RGGNCCY 1 cut(s) 662
EcoRI GAATTC 1 cut(s) 406
EcoRII CCWGG 1 cut(s) 657
EcoT14I CCWWGG 3 cut(s) 210, 411, 818
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 3 cut(s) 210, 411, 818
FaeI CATG 4 cut(s) 49, 194, 710, 862
FaqI GGGAC 2 cut(s) 334, 675
FatI CATG 4 cut(s) 45, 190, 706, 858
FbaI TGATCA 1 cut(s) 115
FblI GTMKAC 1 cut(s) 50
FokI GGATG 5 cut(s) 390, 587, 811, 824, 838
FriOI GRGCYC 1 cut(s) 129
FspBI CTAG 4 cut(s) 195, 546, 632, 746
FspI TGCGCA 1 cut(s) 612
GlaI GCGC 2 cut(s) 612, 925
GsaI CCCAGC 2 cut(s) 172, 321
GsuI CTGGAG 1 cut(s) 36
HaeIII GGCC 4 cut(s) 167, 177, 209, 382
HgaI GACGC 1 cut(s) 546
HhaI GCGC 2 cut(s) 613, 926
Hin1I GRCGYC 1 cut(s) 538
Hin1II CATG 4 cut(s) 49, 194, 710, 862
Hin6I GCGC 2 cut(s) 611, 924
HinP1I GCGC 2 cut(s) 611, 924
HincII GTYRAC 2 cut(s) 51, 601
HindII GTYRAC 2 cut(s) 51, 601
HinfI GANTC 7 cut(s) 92, 110, 142, 187, 199, 338, 855
HpaI GTTAAC 1 cut(s) 601
HphI GGTGA 3 cut(s) 476, 577, 803
Hpy166II GTNNAC 2 cut(s) 51, 601
Hpy188I TCNGA 7 cut(s) 88, 115, 392, 457, 590, 871, 891
Hpy188III TCNNGA 4 cut(s) 15, 506, 701, 859
Hpy8I GTNNAC 2 cut(s) 51, 601
Hpy99I CGWCG 6 cut(s) 250, 271, 274, 277, 280, 283
HpyAV CCTTC 5 cut(s) 481, 556, 585, 589, 782
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 1 cut(s) 688
HpyCH4V TGCA 7 cut(s) 4, 471, 518, 671, 767, 898, 911
HpyF10VI GCNNNNNNNGC 4 cut(s) 156, 257, 356, 359
HpyF3I CTNAG 6 cut(s) 87, 240, 324, 525, 851, 870
HpySE526I ACGT 1 cut(s) 688
Hsp92I GRCGYC 1 cut(s) 538
Hsp92II CATG 4 cut(s) 49, 194, 710, 862
HspAI GCGC 2 cut(s) 611, 924
KflI GGGWCCC 1 cut(s) 662
Ksp22I TGATCA 1 cut(s) 115
KspAI GTTAAC 1 cut(s) 601
Kzo9I GATC 2 cut(s) 115, 752
LmnI GCTCC 2 cut(s) 703, 892
Lsp1109I GCAGC 4 cut(s) 244, 346, 630, 882
LweI GCATC 2 cut(s) 789, 911
MaeI CTAG 4 cut(s) 195, 546, 632, 746
MaeII ACGT 1 cut(s) 688
MaeIII GTNAC 3 cut(s) 37, 310, 791
MalI GATC 2 cut(s) 117, 754
MboI GATC 2 cut(s) 115, 752
MboII GAAGA 8 cut(s) 75, 255, 276, 279, 282, 423, 791, 856
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 2 cut(s) 406, 680
MlyI GAGTC 2 cut(s) 119, 151
MmeI TCCRAC 1 cut(s) 624
MroXI GAANNNNTTC 1 cut(s) 406
MseI TTAA 2 cut(s) 450, 600
MspR9I CCNGG 1 cut(s) 659
Mva1269I GAATGC 2 cut(s) 473, 913
MvaI CCWGG 1 cut(s) 659
MvnI CGCG 1 cut(s) 926
MwoI GCNNNNNNNGC 4 cut(s) 156, 257, 356, 359
NdeII GATC 2 cut(s) 115, 752
NlaIII CATG 4 cut(s) 49, 194, 710, 862
NlaIV GGNNCC 2 cut(s) 663, 664
NmeAIII GCCGAG 1 cut(s) 126
NmuCI GTSAC 2 cut(s) 37, 791
NsbI TGCGCA 1 cut(s) 612
NspI RCATGY 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 122
PagI TCATGA 1 cut(s) 858
PciI ACATGT 1 cut(s) 45
PcsI WCGNNNNNNNCGW 3 cut(s) 272, 275, 278
PctI GAATGC 2 cut(s) 473, 913
PdmI GAANNNNTTC 1 cut(s) 406
PfeI GAWTC 5 cut(s) 92, 187, 199, 338, 855
PflFI GACNNNGTC 1 cut(s) 55
PleI GAGTC 2 cut(s) 118, 150
PpsI GAGTC 2 cut(s) 118, 150
PpuMI RGGWCCY 1 cut(s) 662
PscI ACATGT 1 cut(s) 45
Psp124BI GAGCTC 1 cut(s) 129
Psp5II RGGWCCY 1 cut(s) 662
Psp6I CCWGG 1 cut(s) 657
PspFI CCCAGC 2 cut(s) 168, 317
PspGI CCWGG 1 cut(s) 657
PspN4I GGNNCC 2 cut(s) 663, 664
PspPI GGNCC 3 cut(s) 166, 176, 662
PspPPI RGGWCCY 1 cut(s) 662
PspXI VCTCGAGB 1 cut(s) 122
PstI CTGCAG 1 cut(s) 900
PstNI CAGNNNCTG 1 cut(s) 440
PsyI GACNNNGTC 1 cut(s) 55
SacI GAGCTC 1 cut(s) 129
SalI GTCGAC 1 cut(s) 49
SaqAI TTAA 2 cut(s) 450, 600
Sau3AI GATC 2 cut(s) 115, 752
Sau96I GGNCC 3 cut(s) 166, 176, 662
SchI GAGTC 2 cut(s) 119, 151
ScrFI CCNGG 1 cut(s) 659
SduI GDGCHC 1 cut(s) 129
SfaNI GCATC 2 cut(s) 789, 911
SfcI CTRYAG 1 cut(s) 896
Sfr274I CTCGAG 1 cut(s) 122
SinI GGWCC 1 cut(s) 662
SlaI CTCGAG 1 cut(s) 122
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SpeI ACTAGT 1 cut(s) 545
Sse9I AATT 2 cut(s) 406, 680
SsiI CCGC 7 cut(s) 150, 156, 164, 251, 254, 347, 350
SspMI CTAG 4 cut(s) 195, 546, 632, 746
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 1 cut(s) 657
StyI CCWWGG 3 cut(s) 210, 411, 818
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 1 cut(s) 691
TaqI TCGA 2 cut(s) 50, 123
TasI AATT 2 cut(s) 406, 680
TauI GCSGC 6 cut(s) 159, 167, 254, 257, 350, 353
TfiI GAWTC 5 cut(s) 92, 187, 199, 338, 855
Tru1I TTAA 2 cut(s) 450, 600
Tru9I TTAA 2 cut(s) 450, 600
TscAI CASTG 2 cut(s) 307, 445
TseFI GTSAC 2 cut(s) 37, 791
TseI GCWGC 4 cut(s) 257, 359, 618, 895
Tsp45I GTSAC 2 cut(s) 37, 791
TspDTI ATGAA 4 cut(s) 407, 419, 639, 792
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 2 cut(s) 307, 445
Tth111I GACNNNGTC 1 cut(s) 55
VpaK11BI GGWCC 1 cut(s) 662
XapI RAATTY 1 cut(s) 406
XceI RCATGY 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XhoI CTCGAG 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 406
XspI CTAG 4 cut(s) 195, 546, 632, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.