Rmu_ssc0000203.1_g000001

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000203.1
OG0011393
Physical Location & Seq
Reverse (-)
2 .. 1813
1812 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 938 bp
ccatggtggtttggttgggtgttcaaaagagaagcaggttgttgtcgttgattcatcctgccatggtggtttggttggcgttgttgattcatcctccaatggtggtttggttgggtgttcaaaagagaagcaggttgttgatccaccctgccatggtggacttggtgggtgtttgaaagagaaggggattggtgatgcatctgatgcatcatccttccaaggtgatttcgaatcagcttgtgcagtggctgacattttgtcttttcacaatgaaacttgtggtttttccaatcagcaaaatgtagagcctcagcatgtcaatgggatttgctctgttggagatggaggagctcatgatggagctttgatgttggcccttagttatctgggtgtgcgggatctccttgttgtggaaagggtttgcagatctctctattctacagttcgtggcgacccccttatgtggagaagtattcacatagatcagccactgaatgagaaaattacagatgaagttcttttgcaattgactgatagggctcaaggaaatttacaatgcttgagcctggtggagtgccctaggatcactgacgatggtctcaagcgtgtacttgaaaataatcctaggcttaccaagttaagtgtgcctggatgtactagactcagtatcgagggcattgtgaatagcttaaaggcttttaagtctaagggtgcacatggtgtgaagcatttacgaattggcggactctatggtgtgacacagaagcattttgaagagttgaaatctttgttgggcactgatagcgagatgcagcagaatgcccgcaagccgcacttttatcaccgagggaacttttatttgtcctgtgatgatgatagtgacattgatatcgaaatgtgccctagatgccagaacttgaggcttgtttatgattgcc

Protein Analysis

312

Amino Acids

33.74

Weight (kDa)

5.83

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 122 - 160 7.4e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 26, 122
AciI CCGC 4 cut(s) 395, 742, 824, 831
AclWI GGATC 3 cut(s) 135, 406, 591
AcsI RAATTY 1 cut(s) 548
AdeI CACNNNGTG 1 cut(s) 720
AfaI GTAC 2 cut(s) 610, 656
AfiI CCNNNNNNNGG 3 cut(s) 153, 410, 463
AgsI TTSAA 6 cut(s) 25, 121, 176, 615, 774, 782
AhdI GACNNNNNGTC 1 cut(s) 257
AjnI CCWGG 2 cut(s) 565, 647
AjuI GAANNNNNNNTTGG 2 cut(s) 774, 806
AluBI AGCT 4 cut(s) 237, 351, 363, 688
AluI AGCT 4 cut(s) 237, 351, 363, 688
Alw21I GWGCWC 2 cut(s) 353, 716
Alw26I GTCTC 1 cut(s) 603
Alw44I GTGCAC 1 cut(s) 712
AlwI GGATC 3 cut(s) 135, 406, 591
AlwNI CAGNNNCTG 2 cut(s) 249, 491
AoxI GGCC 1 cut(s) 373
ApaLI GTGCAC 1 cut(s) 712
ApeKI GCWGC 1 cut(s) 812
ApoI RAATTY 1 cut(s) 548
AspA2I CCTAGG 2 cut(s) 579, 624
AspS9I GGNCC 1 cut(s) 374
AsuHPI GGTGA 3 cut(s) 204, 234, 834
AsuII TTCGAA 1 cut(s) 229
AvrII CCTAGG 2 cut(s) 579, 624
BaeGI GKGCMC 4 cut(s) 579, 716, 798, 903
BanII GRGCYC 2 cut(s) 353, 542
Bbv12I GWGCWC 2 cut(s) 353, 716
BbvCI CCTCAGC 1 cut(s) 310
BbvI GCAGC 1 cut(s) 824
BccI CCATC 3 cut(s) 336, 351, 588
BciT130I CCWGG 2 cut(s) 567, 649
BcoDI GTCTC 1 cut(s) 603
BfaI CTAG 4 cut(s) 580, 625, 658, 904
BfmI CTRYAG 1 cut(s) 439
BfuAI ACCTGC 2 cut(s) 26, 122
BglII AGATCT 1 cut(s) 426
BisI GCNGC 2 cut(s) 813, 831
BlnI CCTAGG 2 cut(s) 579, 624
BlsI GCNGC 2 cut(s) 814, 832
Bme1390I CCNGG 2 cut(s) 567, 649
BmeRI GACNNNNNGTC 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 374
BmrFI CCNGG 2 cut(s) 567, 649
BmsI GCATC 6 cut(s) 185, 194, 207, 216, 799, 897
BoxI GACNNNNGTC 1 cut(s) 595
Bpu10I CCTNAGC 1 cut(s) 310
Bpu14I TTCGAA 1 cut(s) 229
BpuEI CTTGAG 4 cut(s) 526, 581, 585, 938
BsaI GGTCTC 1 cut(s) 603
BsaJI CCNNGG 7 cut(s) 2, 62, 152, 218, 579, 624, 845
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 410, 463
BseBI CCWGG 2 cut(s) 567, 649
BseDI CCNNGG 7 cut(s) 2, 62, 152, 218, 579, 624, 845
BseGI GGATG 4 cut(s) 54, 90, 210, 657
BseLI CCNNNNNNNGG 3 cut(s) 153, 410, 463
BseMII CTCAG 2 cut(s) 324, 677
BseRI GAGGAG 1 cut(s) 361
BseSI GKGCMC 4 cut(s) 579, 716, 798, 903
BseXI GCAGC 1 cut(s) 824
BsgI GTGCAG 1 cut(s) 262
BshFI GGCC 1 cut(s) 375
BsiHKAI GWGCWC 2 cut(s) 353, 716
BslI CCNNNNNNNGG 3 cut(s) 153, 410, 463
BsmAI GTCTC 1 cut(s) 603
BsmI GAATGC 1 cut(s) 824
BsnI GGCC 1 cut(s) 375
Bso31I GGTCTC 1 cut(s) 603
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 6 cut(s) 353, 542, 579, 716, 798, 903
Bsp143I GATC 5 cut(s) 140, 398, 426, 482, 583
Bsp19I CCATGG 3 cut(s) 2, 62, 152
BspACI CCGC 4 cut(s) 395, 742, 824, 831
BspANI GGCC 1 cut(s) 375
BspCNI CTCAG 2 cut(s) 323, 676
BspHI TCATGA 1 cut(s) 353
BspMI ACCTGC 2 cut(s) 26, 122
BspPI GGATC 3 cut(s) 135, 406, 591
BspT104I TTCGAA 1 cut(s) 229
BspTNI GGTCTC 1 cut(s) 603
BssECI CCNNGG 7 cut(s) 2, 62, 152, 218, 579, 624, 845
BssMI GATC 5 cut(s) 140, 398, 426, 482, 583
BssT1I CCWWGG 6 cut(s) 2, 62, 152, 218, 579, 624
Bst2UI CCWGG 2 cut(s) 567, 649
Bst4CI ACNGT 1 cut(s) 443
Bst6I CTCTTC 1 cut(s) 769
BstAPI GCANNNNNTGC 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 824, 828
BstDEI CTNAG 4 cut(s) 310, 378, 663, 706
BstDSI CCRYGG 3 cut(s) 2, 62, 152
BstF5I GGATG 4 cut(s) 54, 90, 210, 657
BstKTI GATC 5 cut(s) 143, 401, 429, 485, 586
BstMAI GTCTC 1 cut(s) 603
BstMBI GATC 5 cut(s) 140, 398, 426, 482, 583
BstMWI GCNNNNNNNGC 3 cut(s) 204, 802, 907
BstNI CCWGG 2 cut(s) 567, 649
BstNSI RCATGY 1 cut(s) 318
BstPAI GACNNNNGTC 1 cut(s) 595
BstSCI CCNGG 2 cut(s) 565, 647
BstSFI CTRYAG 1 cut(s) 439
BstSLI GKGCMC 4 cut(s) 579, 716, 798, 903
BstV1I GCAGC 1 cut(s) 824
BstX2I RGATCY 2 cut(s) 398, 426
BstYI RGATCY 2 cut(s) 398, 426
BsuRI GGCC 1 cut(s) 375
BtgI CCRYGG 3 cut(s) 2, 62, 152
BtsCI GGATG 4 cut(s) 54, 90, 210, 657
BtsI GCAGTG 1 cut(s) 250
BtsIMutI CAGTG 4 cut(s) 250, 489, 586, 796
BveI ACCTGC 2 cut(s) 26, 122
Cac8I GCNNGC 2 cut(s) 824, 828
CaiI CAGNNNCTG 2 cut(s) 249, 491
CciI TCATGA 1 cut(s) 353
Cfr13I GGNCC 1 cut(s) 374
Csp6I GTAC 2 cut(s) 609, 655
CviAII CATG 6 cut(s) 3, 63, 153, 315, 354, 717
CviQI GTAC 2 cut(s) 609, 655
DdeI CTNAG 4 cut(s) 310, 378, 663, 706
DpnI GATC 5 cut(s) 142, 400, 428, 484, 585
DpnII GATC 5 cut(s) 140, 398, 426, 482, 583
DraIII CACNNNGTG 1 cut(s) 720
DriI GACNNNNNGTC 1 cut(s) 257
Eam1104I CTCTTC 1 cut(s) 769
Eam1105I GACNNNNNGTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 769
EciI GGCGGA 1 cut(s) 757
Ecl136II GAGCTC 1 cut(s) 351
Eco130I CCWWGG 6 cut(s) 2, 62, 152, 218, 579, 624
Eco24I GRGCYC 2 cut(s) 353, 542
Eco31I GGTCTC 1 cut(s) 603
Eco32I GATATC 1 cut(s) 890
Eco53kI GAGCTC 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 351
EcoRII CCWGG 2 cut(s) 565, 647
EcoRV GATATC 1 cut(s) 890
EcoT14I CCWWGG 6 cut(s) 2, 62, 152, 218, 579, 624
EcoT22I ATGCAT 2 cut(s) 200, 209
EcoT38I GRGCYC 2 cut(s) 353, 542
ErhI CCWWGG 6 cut(s) 2, 62, 152, 218, 579, 624
FaeI CATG 6 cut(s) 6, 66, 156, 318, 357, 720
FalI AAGNNNNNCTT 2 cut(s) 819, 851
FatI CATG 6 cut(s) 2, 62, 152, 314, 353, 716
FauI CCCGC 2 cut(s) 388, 831
Fnu4HI GCNGC 2 cut(s) 813, 831
FokI GGATG 4 cut(s) 41, 77, 197, 664
FriOI GRGCYC 2 cut(s) 353, 542
Fsp4HI GCNGC 2 cut(s) 813, 831
FspBI CTAG 4 cut(s) 580, 625, 658, 904
GluI GCNGC 2 cut(s) 813, 831
HaeIII GGCC 1 cut(s) 375
Hin1II CATG 6 cut(s) 6, 66, 156, 318, 357, 720
HinfI GANTC 5 cut(s) 51, 87, 231, 661, 745
HphI GGTGA 3 cut(s) 204, 234, 834
Hpy166II GTNNAC 3 cut(s) 159, 609, 714
Hpy188I TCNGA 1 cut(s) 203
Hpy188III TCNNGA 1 cut(s) 354
Hpy8I GTNNAC 3 cut(s) 159, 609, 714
HpyAV CCTTC 2 cut(s) 176, 224
HpyCH4III ACNGT 1 cut(s) 443
HpyCH4V TGCA 7 cut(s) 198, 207, 243, 424, 524, 714, 812
HpyF10VI GCNNNNNNNGC 3 cut(s) 204, 802, 907
HpyF3I CTNAG 4 cut(s) 310, 378, 663, 706
Hsp92II CATG 6 cut(s) 6, 66, 156, 318, 357, 720
Kzo9I GATC 5 cut(s) 140, 398, 426, 482, 583
LmnI GCTCC 2 cut(s) 348, 360
Lsp1109I GCAGC 1 cut(s) 824
LweI GCATC 6 cut(s) 185, 194, 207, 216, 799, 897
MaeI CTAG 4 cut(s) 580, 625, 658, 904
MaeIII GTNAC 2 cut(s) 755, 879
MalI GATC 5 cut(s) 142, 400, 428, 484, 585
MboI GATC 5 cut(s) 140, 398, 426, 482, 583
MboII GAAGA 1 cut(s) 786
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 2 cut(s) 398, 426
MhlI GDGCHC 6 cut(s) 353, 542, 579, 716, 798, 903
MluCI AATT 4 cut(s) 502, 525, 548, 736
MlyI GAGTC 2 cut(s) 655, 739
MmeI TCCRAC 1 cut(s) 317
MnlI CCTC 6 cut(s) 104, 319, 339, 665, 840, 913
Mph1103I ATGCAT 2 cut(s) 200, 209
MseI TTAA 3 cut(s) 639, 690, 700
MslI CAYNNNNRTG 1 cut(s) 319
MspR9I CCNGG 2 cut(s) 567, 649
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 824
MvaI CCWGG 2 cut(s) 567, 649
MwoI GCNNNNNNNGC 3 cut(s) 204, 802, 907
NcoI CCATGG 3 cut(s) 2, 62, 152
NdeII GATC 5 cut(s) 140, 398, 426, 482, 583
NlaIII CATG 6 cut(s) 6, 66, 156, 318, 357, 720
NmuCI GTSAC 2 cut(s) 755, 879
NsiI ATGCAT 2 cut(s) 200, 209
NspI RCATGY 1 cut(s) 318
NspV TTCGAA 1 cut(s) 229
PagI TCATGA 1 cut(s) 353
PctI GAATGC 1 cut(s) 824
PfeI GAWTC 3 cut(s) 51, 87, 231
PkrI GCNGC 2 cut(s) 814, 832
PleI GAGTC 2 cut(s) 655, 739
PpsI GAGTC 2 cut(s) 655, 739
PshAI GACNNNNGTC 1 cut(s) 595
Psp124BI GAGCTC 1 cut(s) 353
Psp6I CCWGG 2 cut(s) 565, 647
PspGI CCWGG 2 cut(s) 565, 647
PspPI GGNCC 1 cut(s) 374
PstNI CAGNNNCTG 2 cut(s) 249, 491
PsuI RGATCY 2 cut(s) 398, 426
RsaI GTAC 2 cut(s) 610, 656
RsaNI GTAC 2 cut(s) 609, 655
RseI CAYNNNNRTG 1 cut(s) 319
SacI GAGCTC 1 cut(s) 353
SaqAI TTAA 3 cut(s) 639, 690, 700
SatI GCNGC 2 cut(s) 813, 831
Sau3AI GATC 5 cut(s) 140, 398, 426, 482, 583
Sau96I GGNCC 1 cut(s) 374
SchI GAGTC 2 cut(s) 655, 739
ScrFI CCNGG 2 cut(s) 567, 649
SduI GDGCHC 6 cut(s) 353, 542, 579, 716, 798, 903
SetI ASST 7 cut(s) 40, 136, 224, 239, 353, 365, 690
SfaNI GCATC 6 cut(s) 185, 194, 207, 216, 799, 897
SfcI CTRYAG 1 cut(s) 439
SfuI TTCGAA 1 cut(s) 229
SmiMI CAYNNNNRTG 1 cut(s) 319
SmlI CTYRAG 4 cut(s) 541, 560, 600, 917
SmoI CTYRAG 4 cut(s) 541, 560, 600, 917
Sse9I AATT 4 cut(s) 502, 525, 548, 736
SsiI CCGC 4 cut(s) 395, 742, 824, 831
SspMI CTAG 4 cut(s) 580, 625, 658, 904
SstI GAGCTC 1 cut(s) 353
StyD4I CCNGG 2 cut(s) 565, 647
StyI CCWWGG 6 cut(s) 2, 62, 152, 218, 579, 624
TaaI ACNGT 1 cut(s) 443
TaqI TCGA 3 cut(s) 229, 670, 892
TasI AATT 4 cut(s) 502, 525, 548, 736
TatI WGTACW 2 cut(s) 608, 654
TauI GCSGC 1 cut(s) 833
TfiI GAWTC 3 cut(s) 51, 87, 231
Tru1I TTAA 3 cut(s) 639, 690, 700
Tru9I TTAA 3 cut(s) 639, 690, 700
TscAI CASTG 4 cut(s) 250, 496, 593, 803
TseFI GTSAC 2 cut(s) 755, 879
TseI GCWGC 1 cut(s) 812
Tsp45I GTSAC 2 cut(s) 755, 879
TspDTI ATGAA 4 cut(s) 43, 79, 286, 526
TspRI CASTG 4 cut(s) 250, 496, 593, 803
VneI GTGCAC 1 cut(s) 712
XapI RAATTY 1 cut(s) 548
XceI RCATGY 1 cut(s) 318
XcmI CCANNNNNNNNNTGG 2 cut(s) 104, 159
XmaJI CCTAGG 2 cut(s) 579, 624
XspI CTAG 4 cut(s) 580, 625, 658, 904
Zsp2I ATGCAT 2 cut(s) 200, 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.