Rmu_ssc0000204.1_g000031

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000204.1
OG0014090
Physical Location & Seq
Reverse (-)
153699 .. 157791
4093 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1809 bp
atgatgagctttggagacattctccgatcggagagagagaaagagatgatgctcaccaccagtacgagtctcgatcacaccatcgtcgagagtgttacagccagtttaggcaaaggcttcgatttaacctcggatttccggcttaagtattgcaaaggcaaacaacggttggtttccctaaacgacggcgacaaaagagagctcgtcgtgcctggttttggttccattccggacgtctccggcgacattaaatgcgacaagggagaccggatgaggtgccagacggacatgttcagcttcagtcagatgtcggagtatatcaaccagaagtgtagtgttcccgggaaagtgccgtcggggcttttcaattcgatgttcgggttcgatagtggttcgtgggggagcgatgcggccaatataaaatgcttgggtgtggatggatatttcatcatcttcttcagtgttcatattgataggtaccctcttgttcttgccgatgaagttcgcgatgctgttccgaccacttgggacccacttgccatttcaagattcatagaaaaatatgggacgcacatcgtggtgggtttgagtgttgggggtcaggatgtggtgttggttaagcaagataaatcatcaaatttggggccatcagacctcaaaactcacttaaacgagcttggagatcagcattttacggggacttgtaatttctccccactcctctccaaagcaaaagaccataaacacaaggcgccacgggcattcaatgtctttgatgctccgcagtctgctgccctcaacggcttctccaccgcaaacacaaaagatggaattactgtgataagctctaaaaagggaggtgatccaacggccagtagccacagcgagtggcttctaacagttccgtcaatgccggacgctattcagtacagcttcattccaatcacttctctccttaaaggtgttcccggcaaaggtttcttgtcccatgccatcaccctctatctcagatgtcctgccacaaatagcaaatctaatccatcatcacctgctattcacttcagcttgatgggtcctaggttatatgtaaacaccacacaggttagagttggaaataatgcccctgtcacagggatgcgcttgtttcttgagggcatgaaatgcaacaggctagccatccacctccaacatctagcgaacactccattgatgttggagaacaagatcgatgacacaacgatgtggcgaggatcagaagagatcgacgatgaccggtactttgaaccaacaaacgggaaaaagttctcccacgtgtgcacggcgccagtaacgtacgacccaaaatggatatcttcgggcataacctcggccttcattgtctcgggagcacaacttgtggtcaagcaacacggctcaaagaatgttctccatcttcggctcttgttctccaaagtcttggactctttcttagtgcaatcaaattgggcagagtcatgttcttcaactaaatcgtcgtcgtctggccttttgaatgcgatgagtagaccaatttctgggaactattggggaaaagagaaagtagaacctgccgcagaggatgtgatattggattcgagtgtttttcctgcagggcctccggtgcctttgccaaggcaaaaatttgtgaagcatgtaaacacctcacatgtatgcaaagggccgcaggatagtcctggccattggttggtcaccggagcaaagctcaacttggtcaagggaaagattggcttgcaagtcaagttctctctgttaaacctagcagaaaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

602

Amino Acids

65.79

Weight (kDa)

8.76

Isoelectric Point (pI)

34.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 121 - 335 8.9e-29 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014090)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14780
fragaria_vesca FvH4_4g33390
malus_domestica MD13G1019100.v1.1
prunus_persica Prupe.1G334800_v2.0.a1
pyrus_communis pycom13g01620 pycom16g01550
rosa_chinensis RchiOBHm_Chr4g0442651
rosa_laevigata RLG00000005994
rosa_multiflora Rmu_co8443405.1_g000001 Rmu_ssc0000204.1_g000031
rosa_roxburghii Rroxscaffold_5G00383460
rosa_rugosa Rorug04G0341900
rosa_samantha Rh4AG394400 Rh4BG407400 Rh4CG422800 Rh4DG401400
rosa_wichuraiana Rw4G034080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1057
AatII GACGTC 1 cut(s) 237
Acc36I ACCTGC 2 cut(s) 1057, 1594
Acc65I GGTACC 1 cut(s) 477
AccB1I GGYRCC 5 cut(s) 276, 477, 751, 1321, 1639
AccB7I CCANNNNNTGG 2 cut(s) 1553, 1723
AccI GTMKAC 1 cut(s) 1543
AccII CGCG 1 cut(s) 507
AccIII TCCGGA 1 cut(s) 229
AciI CCGC 5 cut(s) 410, 782, 813, 1590, 1700
AclWI GGATC 2 cut(s) 857, 1258
AcoI YGGCCR 3 cut(s) 411, 870, 1714
AcsI RAATTY 2 cut(s) 637, 1658
AcuI CTGAAG 3 cut(s) 283, 442, 1045
AcvI CACGTG 1 cut(s) 1312
AcyI GRCGYC 3 cut(s) 234, 752, 1322
AdeI CACNNNGTG 1 cut(s) 577
AfaI GTAC 5 cut(s) 64, 479, 929, 1277, 1334
AfiI CCNNNNNNNGG 6 cut(s) 218, 974, 1130, 1292, 1553, 1723
AflII CTTAAG 1 cut(s) 143
AflIII ACRYGT 3 cut(s) 288, 1311, 1684
AgeI ACCGGT 1 cut(s) 1272
AgsI TTSAA 6 cut(s) 367, 546, 766, 1283, 1503, 1531
AjnI CCWGG 2 cut(s) 211, 1711
AluBI AGCT 8 cut(s) 9, 202, 297, 676, 846, 933, 1065, 1741
AluI AGCT 8 cut(s) 9, 202, 297, 676, 846, 933, 1065, 1741
Alw21I GWGCWC 3 cut(s) 204, 1319, 1390
Alw26I GTCTC 5 cut(s) 9, 74, 241, 258, 1384
Alw44I GTGCAC 1 cut(s) 1315
AlwI GGATC 2 cut(s) 857, 1258
Ama87I CYCGRG 2 cut(s) 341, 1381
Aor13HI TCCGGA 1 cut(s) 229
AoxI GGCC 8 cut(s) 411, 644, 870, 1368, 1522, 1631, 1697, 1714
ApaLI GTGCAC 1 cut(s) 1315
ApeKI GCWGC 1 cut(s) 791
ApoI RAATTY 2 cut(s) 637, 1658
ArsI GACNNNNNNTTYG 2 cut(s) 1262, 1294
AsiGI ACCGGT 1 cut(s) 1272
Asp700I GAANNNNTTC 1 cut(s) 1301
Asp718I GGTACC 1 cut(s) 477
AspA2I CCTAGG 1 cut(s) 1076
AspLEI GCGC 3 cut(s) 754, 1140, 1324
AspS9I GGNCC 5 cut(s) 529, 644, 1073, 1631, 1697
AsuC2I CCSGG 3 cut(s) 342, 343, 969
AsuHPI GGTGA 5 cut(s) 46, 872, 988, 1038, 1720
AsuNHI GCTAGC 1 cut(s) 1171
AvaI CYCGRG 2 cut(s) 341, 1381
AvaII GGWCC 2 cut(s) 529, 1073
AvrII CCTAGG 1 cut(s) 1076
BaeGI GKGCMC 1 cut(s) 1319
BalI TGGCCA 1 cut(s) 1716
BanI GGYRCC 5 cut(s) 276, 477, 751, 1321, 1639
BanII GRGCYC 1 cut(s) 204
BbrPI CACGTG 1 cut(s) 1312
Bbv12I GWGCWC 3 cut(s) 204, 1319, 1390
BbvI GCAGC 1 cut(s) 778
BccI CCATC 9 cut(s) 89, 431, 655, 821, 1001, 1048, 1063, 1184, 1437
BceAI ACGGC 6 cut(s) 202, 337, 817, 885, 1335, 1426
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 2 cut(s) 213, 1713
BcnI CCSGG 3 cut(s) 342, 343, 969
BcoDI GTCTC 5 cut(s) 9, 74, 241, 258, 1384
BfaI CTAG 4 cut(s) 1077, 1172, 1193, 1796
BfmI CTRYAG 1 cut(s) 1626
BfoI RGCGCY 2 cut(s) 755, 1325
BfrI CTTAAG 1 cut(s) 143
BfuAI ACCTGC 2 cut(s) 1057, 1594
BglI GCCNNNNNGGC 1 cut(s) 358
BisI GCNGC 4 cut(s) 411, 792, 1590, 1700
BlnI CCTAGG 1 cut(s) 1076
BlsI GCNGC 4 cut(s) 412, 793, 1591, 1701
Bme1390I CCNGG 5 cut(s) 213, 342, 343, 969, 1713
Bme18I GGWCC 2 cut(s) 529, 1073
BmeT110I CYCGRG 2 cut(s) 341, 1381
BmgT120I GGNCC 5 cut(s) 529, 644, 1073, 1631, 1697
BmrFI CCNGG 5 cut(s) 213, 342, 343, 969, 1713
BmsI GCATC 5 cut(s) 39, 397, 499, 766, 1125
BmtI GCTAGC 1 cut(s) 1175
BplI GAGNNNNNCTC 3 cut(s) 38, 1725, 1757
BpuEI CTTGAG 1 cut(s) 1169
BpuMI CCSGG 3 cut(s) 342, 343, 969
Bsa29I ATCGAT 1 cut(s) 1227
BsaAI YACGTR 1 cut(s) 1312
BsaHI GRCGYC 3 cut(s) 234, 752, 1322
BsaI GGTCTC 1 cut(s) 258
BsaJI CCNNGG 6 cut(s) 129, 341, 755, 1076, 1365, 1649
BsaWI WCCGGW 5 cut(s) 229, 267, 1272, 1636, 1730
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 6 cut(s) 218, 974, 1130, 1292, 1553, 1723
Bse118I RCCGGY 1 cut(s) 1272
Bse1I ACTGG 4 cut(s) 60, 102, 873, 1325
BseAI TCCGGA 1 cut(s) 229
BseBI CCWGG 2 cut(s) 213, 1713
BseCI ATCGAT 1 cut(s) 1227
BseDI CCNNGG 6 cut(s) 129, 341, 755, 1076, 1365, 1649
BseGI GGATG 6 cut(s) 276, 442, 610, 1140, 1176, 1603
BseLI CCNNNNNNNGG 6 cut(s) 218, 974, 1130, 1292, 1553, 1723
BseMII CTCAG 1 cut(s) 1021
BseNI ACTGG 4 cut(s) 60, 102, 873, 1325
BseRI GAGGAG 1 cut(s) 710
BseSI GKGCMC 1 cut(s) 1319
BseXI GCAGC 1 cut(s) 778
Bsh1236I CGCG 1 cut(s) 507
Bsh1285I CGRYCG 1 cut(s) 29
BshFI GGCC 8 cut(s) 413, 646, 872, 1370, 1524, 1633, 1699, 1716
BshNI GGYRCC 5 cut(s) 276, 477, 751, 1321, 1639
BshTI ACCGGT 1 cut(s) 1272
BshVI ATCGAT 1 cut(s) 1227
BsiEI CGRYCG 1 cut(s) 29
BsiHKAI GWGCWC 3 cut(s) 204, 1319, 1390
BsiHKCI CYCGRG 2 cut(s) 341, 1381
BsiWI CGTACG 1 cut(s) 1332
BslFI GGGAC 4 cut(s) 542, 580, 712, 970
BslI CCNNNNNNNGG 6 cut(s) 218, 974, 1130, 1292, 1553, 1723
BsmAI GTCTC 5 cut(s) 9, 74, 241, 258, 1384
BsmBI CGTCTC 1 cut(s) 241
BsmFI GGGAC 4 cut(s) 542, 580, 712, 970
BsmI GAATGC 2 cut(s) 761, 1537
BsnI GGCC 8 cut(s) 413, 646, 872, 1370, 1524, 1633, 1699, 1716
Bso31I GGTCTC 1 cut(s) 258
BsoBI CYCGRG 2 cut(s) 341, 1381
Bsp1286I GDGCHC 3 cut(s) 204, 1319, 1390
Bsp13I TCCGGA 1 cut(s) 229
Bsp143I GATC 7 cut(s) 26, 73, 682, 862, 1224, 1250, 1260
Bsp68I TCGCGA 1 cut(s) 507
BspACI CCGC 5 cut(s) 410, 782, 813, 1590, 1700
BspANI GGCC 8 cut(s) 413, 646, 872, 1370, 1524, 1633, 1699, 1716
BspCNI CTCAG 1 cut(s) 1020
BspDI ATCGAT 1 cut(s) 1227
BspEI TCCGGA 1 cut(s) 229
BspFNI CGCG 1 cut(s) 507
BspMAI CTGCAG 1 cut(s) 1630
BspMI ACCTGC 2 cut(s) 1057, 1594
BspOI GCTAGC 1 cut(s) 1175
BspPI GGATC 2 cut(s) 857, 1258
BspT107I GGYRCC 5 cut(s) 276, 477, 751, 1321, 1639
BspTI CTTAAG 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 258
BsrFI RCCGGY 1 cut(s) 1272
BsrI ACTGG 4 cut(s) 60, 102, 873, 1325
BssAI RCCGGY 1 cut(s) 1272
BssECI CCNNGG 6 cut(s) 129, 341, 755, 1076, 1365, 1649
BssMI GATC 7 cut(s) 26, 73, 682, 862, 1224, 1250, 1260
BssNI GRCGYC 3 cut(s) 234, 752, 1322
BssT1I CCWWGG 2 cut(s) 1076, 1649
Bst2UI CCWGG 2 cut(s) 213, 1713
Bst4CI ACNGT 3 cut(s) 168, 838, 901
Bst6I CTCTTC 1 cut(s) 1251
BstACI GRCGYC 3 cut(s) 234, 752, 1322
BstAFI CTTAAG 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 1161
BstBAI YACGTR 1 cut(s) 1312
BstC8I GCNNGC 2 cut(s) 1173, 1769
BstDEI CTNAG 2 cut(s) 1007, 1468
BstDSI CCRYGG 1 cut(s) 755
BstEII GGTNACC 1 cut(s) 1726
BstENI CCTNNNNNAGG 1 cut(s) 1128
BstF5I GGATG 6 cut(s) 276, 442, 610, 1140, 1176, 1603
BstFNI CGCG 1 cut(s) 507
BstH2I RGCGCY 2 cut(s) 755, 1325
BstHHI GCGC 3 cut(s) 754, 1140, 1324
BstKTI GATC 7 cut(s) 29, 76, 685, 865, 1227, 1253, 1263
BstMAI GTCTC 5 cut(s) 9, 74, 241, 258, 1384
BstMBI GATC 7 cut(s) 26, 73, 682, 862, 1224, 1250, 1260
BstMCI CGRYCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 5 cut(s) 208, 358, 758, 1161, 1639
BstNI CCWGG 2 cut(s) 213, 1713
BstNSI RCATGY 3 cut(s) 292, 1673, 1688
BstPI GGTNACC 1 cut(s) 1726
BstSCI CCNGG 5 cut(s) 211, 340, 341, 967, 1711
BstSFI CTRYAG 1 cut(s) 1626
BstSLI GKGCMC 1 cut(s) 1319
BstUI CGCG 1 cut(s) 507
BstV1I GCAGC 1 cut(s) 778
BstXI CCANNNNNNTGG 1 cut(s) 1456
Bsu15I ATCGAT 1 cut(s) 1227
BsuRI GGCC 8 cut(s) 413, 646, 872, 1370, 1524, 1633, 1699, 1716
BsuTUI ATCGAT 1 cut(s) 1227
BtgI CCRYGG 1 cut(s) 755
BtgZI GCGATG 3 cut(s) 420, 522, 1550
BtsCI GGATG 6 cut(s) 276, 442, 610, 1140, 1176, 1603
BtsIMutI CAGTG 1 cut(s) 466
BtuMI TCGCGA 1 cut(s) 507
BveI ACCTGC 2 cut(s) 1057, 1594
Cac8I GCNNGC 2 cut(s) 1173, 1769
CfoI GCGC 3 cut(s) 754, 1140, 1324
Cfr10I RCCGGY 1 cut(s) 1272
Cfr13I GGNCC 5 cut(s) 529, 644, 1073, 1631, 1697
Cfr9I CCCGGG 1 cut(s) 341
ClaI ATCGAT 1 cut(s) 1227
CseI GACGC 2 cut(s) 577, 926
Csp6I GTAC 5 cut(s) 63, 478, 928, 1276, 1333
CspAI ACCGGT 1 cut(s) 1272
CviAII CATG 6 cut(s) 289, 989, 1156, 1494, 1670, 1685
CviQI GTAC 5 cut(s) 63, 478, 928, 1276, 1333
DdeI CTNAG 2 cut(s) 1007, 1468
DinI GGCGCC 2 cut(s) 753, 1323
DpnI GATC 7 cut(s) 28, 75, 684, 864, 1226, 1252, 1262
DpnII GATC 7 cut(s) 26, 73, 682, 862, 1224, 1250, 1260
DraIII CACNNNGTG 1 cut(s) 577
EaeI YGGCCR 3 cut(s) 411, 870, 1714
Eam1104I CTCTTC 1 cut(s) 1251
EarI CTCTTC 1 cut(s) 1251
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 2 cut(s) 1076, 1649
Eco24I GRGCYC 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 258
Eco32I GATATC 1 cut(s) 1350
Eco47I GGWCC 2 cut(s) 529, 1073
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 3 cut(s) 283, 442, 1045
Eco72I CACGTG 1 cut(s) 1312
Eco88I CYCGRG 2 cut(s) 341, 1381
Eco91I GGTNACC 1 cut(s) 1726
EcoICRI GAGCTC 1 cut(s) 202
EcoNI CCTNNNNNAGG 1 cut(s) 1128
EcoO109I RGGNCCY 3 cut(s) 529, 1073, 1631
EcoO65I GGTNACC 1 cut(s) 1726
EcoRII CCWGG 2 cut(s) 211, 1711
EcoRV GATATC 1 cut(s) 1350
EcoT14I CCWWGG 2 cut(s) 1076, 1649
EcoT38I GRGCYC 1 cut(s) 204
EgeI GGCGCC 2 cut(s) 753, 1323
EheI GGCGCC 2 cut(s) 753, 1323
ErhI CCWWGG 2 cut(s) 1076, 1649
Esp3I CGTCTC 1 cut(s) 241
FaeI CATG 6 cut(s) 292, 992, 1159, 1497, 1673, 1688
FalI AAGNNNNNCTT 4 cut(s) 1730, 1762, 1751, 1783
FaqI GGGAC 4 cut(s) 542, 580, 712, 970
FatI CATG 6 cut(s) 288, 988, 1155, 1493, 1669, 1684
FblI GTMKAC 1 cut(s) 1543
Fnu4HI GCNGC 4 cut(s) 411, 792, 1590, 1700
FokI GGATG 6 cut(s) 283, 449, 617, 1147, 1163, 1610
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 4 cut(s) 411, 792, 1590, 1700
FspBI CTAG 4 cut(s) 1077, 1172, 1193, 1796
GlaI GCGC 3 cut(s) 753, 1139, 1323
GluI GCNGC 4 cut(s) 411, 792, 1590, 1700
HaeII RGCGCY 2 cut(s) 755, 1325
HaeIII GGCC 8 cut(s) 413, 646, 872, 1370, 1524, 1633, 1699, 1716
HgaI GACGC 2 cut(s) 577, 926
HhaI GCGC 3 cut(s) 754, 1140, 1324
Hin1I GRCGYC 3 cut(s) 234, 752, 1322
Hin1II CATG 6 cut(s) 292, 992, 1159, 1497, 1673, 1688
Hin6I GCGC 3 cut(s) 752, 1138, 1322
HinP1I GCGC 3 cut(s) 752, 1138, 1322
HinfI GANTC 5 cut(s) 67, 549, 1460, 1490, 1610
HphI GGTGA 5 cut(s) 46, 872, 988, 1038, 1720
Hpy166II GTNNAC 4 cut(s) 1090, 1317, 1544, 1675
Hpy188I TCNGA 9 cut(s) 26, 31, 133, 306, 313, 519, 652, 1010, 1255
Hpy188III TCNNGA 8 cut(s) 71, 88, 230, 506, 546, 602, 1148, 1383
Hpy8I GTNNAC 4 cut(s) 1090, 1317, 1544, 1675
Hpy99I CGWCG 7 cut(s) 89, 188, 209, 358, 1268, 1516, 1519
HpyAV CCTTC 1 cut(s) 1381
HpyCH4III ACNGT 3 cut(s) 168, 838, 901
HpyCH4IV ACGT 3 cut(s) 234, 1311, 1331
HpyCH4V TGCA 7 cut(s) 153, 1164, 1317, 1474, 1628, 1692, 1771
HpyF10VI GCNNNNNNNGC 5 cut(s) 208, 358, 758, 1161, 1639
HpyF3I CTNAG 2 cut(s) 1007, 1468
HpySE526I ACGT 3 cut(s) 234, 1311, 1331
Hsp92I GRCGYC 3 cut(s) 234, 752, 1322
Hsp92II CATG 6 cut(s) 292, 992, 1159, 1497, 1673, 1688
HspAI GCGC 3 cut(s) 752, 1138, 1322
KasI GGCGCC 2 cut(s) 751, 1321
KflI GGGWCCC 1 cut(s) 529
Kpn2I TCCGGA 1 cut(s) 229
KpnI GGTACC 1 cut(s) 481
Kzo9I GATC 7 cut(s) 26, 73, 682, 862, 1224, 1250, 1260
LmnI GCTCC 4 cut(s) 402, 784, 1385, 1733
Lsp1109I GCAGC 1 cut(s) 778
LweI GCATC 5 cut(s) 39, 397, 499, 766, 1125
MaeI CTAG 4 cut(s) 1077, 1172, 1193, 1796
MaeII ACGT 3 cut(s) 234, 1311, 1331
MaeIII GTNAC 4 cut(s) 94, 1126, 1327, 1726
MalI GATC 7 cut(s) 28, 75, 684, 864, 1226, 1252, 1262
MboI GATC 7 cut(s) 26, 73, 682, 862, 1224, 1250, 1260
MboII GAAGA 6 cut(s) 445, 448, 1268, 1344, 1424, 1491
MhlI GDGCHC 3 cut(s) 204, 1319, 1390
MlsI TGGCCA 1 cut(s) 1716
MluCI AATT 7 cut(s) 367, 637, 706, 831, 1480, 1548, 1658
MluNI TGGCCA 1 cut(s) 1716
Mly113I GGCGCC 2 cut(s) 752, 1322
MlyI GAGTC 3 cut(s) 76, 1454, 1499
MmeI TCCRAC 6 cut(s) 291, 542, 890, 1090, 1194, 1210
Mox20I TGGCCA 1 cut(s) 1716
MroI TCCGGA 1 cut(s) 229
MroXI GAANNNNTTC 1 cut(s) 1301
MscI TGGCCA 1 cut(s) 1716
MseI TTAA 7 cut(s) 125, 144, 249, 618, 668, 957, 1790
MslI CAYNNNNRTG 4 cut(s) 578, 1133, 1238, 1687
Msp20I TGGCCA 1 cut(s) 1716
MspCI CTTAAG 1 cut(s) 143
MspR9I CCNGG 5 cut(s) 213, 342, 343, 969, 1713
Mva1269I GAATGC 2 cut(s) 761, 1537
MvaI CCWGG 2 cut(s) 213, 1713
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 5 cut(s) 208, 358, 758, 1161, 1639
NarI GGCGCC 2 cut(s) 752, 1322
NciI CCSGG 3 cut(s) 342, 343, 969
NdeII GATC 7 cut(s) 26, 73, 682, 862, 1224, 1250, 1260
NheI GCTAGC 1 cut(s) 1171
NlaIII CATG 6 cut(s) 292, 992, 1159, 1497, 1673, 1688
NmeAIII GCCGAG 1 cut(s) 1346
NmuCI GTSAC 2 cut(s) 1126, 1726
NruI TCGCGA 1 cut(s) 507
NspI RCATGY 3 cut(s) 292, 1673, 1688
PaqCI CACCTGC 1 cut(s) 1057
PciI ACATGT 2 cut(s) 288, 1684
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 2 cut(s) 761, 1537
PdmI GAANNNNTTC 1 cut(s) 1301
PfeI GAWTC 2 cut(s) 549, 1610
Pfl23II CGTACG 1 cut(s) 1332
PflMI CCANNNNNTGG 2 cut(s) 1553, 1723
PinAI ACCGGT 1 cut(s) 1272
PkrI GCNGC 4 cut(s) 412, 793, 1591, 1701
Ple19I CGATCG 1 cut(s) 29
PleI GAGTC 3 cut(s) 75, 1454, 1498
PluTI GGCGCC 2 cut(s) 755, 1325
PmaCI CACGTG 1 cut(s) 1312
PmlI CACGTG 1 cut(s) 1312
PpsI GAGTC 3 cut(s) 75, 1454, 1498
Ppu21I YACGTR 1 cut(s) 1312
PpuMI RGGWCCY 2 cut(s) 529, 1073
PscI ACATGT 2 cut(s) 288, 1684
Psp124BI GAGCTC 1 cut(s) 204
Psp5II RGGWCCY 2 cut(s) 529, 1073
Psp6I CCWGG 2 cut(s) 211, 1711
PspCI CACGTG 1 cut(s) 1312
PspEI GGTNACC 1 cut(s) 1726
PspGI CCWGG 2 cut(s) 211, 1711
PspLI CGTACG 1 cut(s) 1332
PspPI GGNCC 5 cut(s) 529, 644, 1073, 1631, 1697
PspPPI RGGWCCY 2 cut(s) 529, 1073
PstI CTGCAG 1 cut(s) 1630
PvuI CGATCG 1 cut(s) 29
RruI TCGCGA 1 cut(s) 507
RsaI GTAC 5 cut(s) 64, 479, 929, 1277, 1334
RsaNI GTAC 5 cut(s) 63, 478, 928, 1276, 1333
RseI CAYNNNNRTG 4 cut(s) 578, 1133, 1238, 1687
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 7 cut(s) 125, 144, 249, 618, 668, 957, 1790
SatI GCNGC 4 cut(s) 411, 792, 1590, 1700
Sau3AI GATC 7 cut(s) 26, 73, 682, 862, 1224, 1250, 1260
Sau96I GGNCC 5 cut(s) 529, 644, 1073, 1631, 1697
SbfI CCTGCAGG 1 cut(s) 1630
SchI GAGTC 3 cut(s) 76, 1454, 1499
ScrFI CCNGG 5 cut(s) 213, 342, 343, 969, 1713
SdaI CCTGCAGG 1 cut(s) 1630
SduI GDGCHC 3 cut(s) 204, 1319, 1390
SfaNI GCATC 5 cut(s) 39, 397, 499, 766, 1125
SfcI CTRYAG 1 cut(s) 1626
SfoI GGCGCC 2 cut(s) 753, 1323
SinI GGWCC 2 cut(s) 529, 1073
SmaI CCCGGG 1 cut(s) 343
SmiMI CAYNNNNRTG 4 cut(s) 578, 1133, 1238, 1687
SmlI CTYRAG 2 cut(s) 143, 1148
SmoI CTYRAG 2 cut(s) 143, 1148
Sse8387I CCTGCAGG 1 cut(s) 1630
Sse9I AATT 7 cut(s) 367, 637, 706, 831, 1480, 1548, 1658
SsiI CCGC 5 cut(s) 410, 782, 813, 1590, 1700
SspDI GGCGCC 2 cut(s) 751, 1321
SspMI CTAG 4 cut(s) 1077, 1172, 1193, 1796
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 5 cut(s) 211, 340, 341, 967, 1711
StyI CCWWGG 2 cut(s) 1076, 1649
TaaI ACNGT 3 cut(s) 168, 838, 901
TaiI ACGT 3 cut(s) 237, 1314, 1334
TaqI TCGA 8 cut(s) 72, 87, 120, 371, 384, 1227, 1263, 1613
TasI AATT 7 cut(s) 367, 637, 706, 831, 1480, 1548, 1658
TatI WGTACW 1 cut(s) 927
TauI GCSGC 3 cut(s) 413, 1592, 1702
TfiI GAWTC 2 cut(s) 549, 1610
Tru1I TTAA 7 cut(s) 125, 144, 249, 618, 668, 957, 1790
Tru9I TTAA 7 cut(s) 125, 144, 249, 618, 668, 957, 1790
TscAI CASTG 1 cut(s) 466
TseFI GTSAC 2 cut(s) 1126, 1726
TseI GCWGC 1 cut(s) 791
Tsp45I GTSAC 2 cut(s) 1126, 1726
TspDTI ATGAA 7 cut(s) 436, 455, 513, 541, 925, 1172, 1363
TspGWI ACGGA 2 cut(s) 299, 894
TspMI CCCGGG 1 cut(s) 341
TspRI CASTG 1 cut(s) 466
Van91I CCANNNNNTGG 2 cut(s) 1553, 1723
Vha464I CTTAAG 1 cut(s) 143
VneI GTGCAC 1 cut(s) 1315
VpaK11BI GGWCC 2 cut(s) 529, 1073
XagI CCTNNNNNAGG 1 cut(s) 1128
XapI RAATTY 2 cut(s) 637, 1658
XceI RCATGY 3 cut(s) 292, 1673, 1688
XmaI CCCGGG 1 cut(s) 341
XmaJI CCTAGG 1 cut(s) 1076
XmiI GTMKAC 1 cut(s) 1543
XmnI GAANNNNTTC 1 cut(s) 1301
XspI CTAG 4 cut(s) 1077, 1172, 1193, 1796
ZraI GACGTC 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.