Rmu_ssc0000295.1_g000014

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000295.1
OG0017760
Physical Location & Seq
Forward (+)
63223 .. 63579
357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000295.1_g000014.1.cds

Sequence Viewer

Length: 357 bp
atggtggtttggggggaggatgttctacttgatggtttggggaattttcgagcaccatttgcagttcatgtatctaatgtttgctctactaagcacactgagctgttgactattaaggaaggtctcaaactgctgcagactgaaaacgtggtactggaaactgactgtcttgaggcgacctatgatatagctcatgtgcagcatgatttgaatgctttaggcagcctggtggatgatataaagactgccttaaggtctattcgtaatgtttcagtatgccatgctccaagaacttgtaatggagtggcatatagactagcaagtattgcttatgaaaccaggaatagttgtctttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

118

Amino Acids

12.97

Weight (kDa)

5.64

Isoelectric Point (pI)

27.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 43
AfaI GTAC 1 cut(s) 153
AflII CTTAAG 1 cut(s) 250
AgsI TTSAA 1 cut(s) 211
AjnI CCWGG 2 cut(s) 225, 338
AluBI AGCT 2 cut(s) 103, 191
AluI AGCT 2 cut(s) 103, 191
Alw21I GWGCWC 1 cut(s) 55
Alw26I GTCTC 1 cut(s) 128
ApeKI GCWGC 3 cut(s) 133, 199, 222
ApoI RAATTY 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 55
BbvI GCAGC 3 cut(s) 120, 211, 234
BccI CCATC 1 cut(s) 26
BciT130I CCWGG 2 cut(s) 227, 340
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 134
BfrI CTTAAG 1 cut(s) 250
BisI GCNGC 3 cut(s) 134, 200, 223
BlsI GCNGC 3 cut(s) 135, 201, 224
Bme1390I CCNGG 2 cut(s) 227, 340
BmrFI CCNGG 2 cut(s) 227, 340
BpuEI CTTGAG 1 cut(s) 191
BsaI GGTCTC 1 cut(s) 128
Bse1I ACTGG 1 cut(s) 159
BseBI CCWGG 2 cut(s) 227, 340
BseGI GGATG 2 cut(s) 25, 238
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 1 cut(s) 159
BseXI GCAGC 3 cut(s) 120, 211, 234
BsgI GTGCAG 1 cut(s) 218
BsiHKAI GWGCWC 1 cut(s) 55
BsmAI GTCTC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 217
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 55
BspCNI CTCAG 1 cut(s) 91
BspMAI CTGCAG 1 cut(s) 138
BspTI CTTAAG 1 cut(s) 250
BspTNI GGTCTC 1 cut(s) 128
BsrI ACTGG 1 cut(s) 159
Bst2UI CCWGG 2 cut(s) 227, 340
Bst4CI ACNGT 1 cut(s) 167
BstAFI CTTAAG 1 cut(s) 250
BstAPI GCANNNNNTGC 2 cut(s) 59, 326
BstDEI CTNAG 2 cut(s) 90, 99
BstF5I GGATG 2 cut(s) 25, 238
BstMAI GTCTC 1 cut(s) 128
BstMWI GCNNNNNNNGC 3 cut(s) 59, 100, 326
BstNI CCWGG 2 cut(s) 227, 340
BstSCI CCNGG 2 cut(s) 225, 338
BstSFI CTRYAG 1 cut(s) 134
BstV1I GCAGC 3 cut(s) 120, 211, 234
BtsCI GGATG 2 cut(s) 25, 238
BtsIMutI CAGTG 1 cut(s) 96
Csp6I GTAC 1 cut(s) 152
CviAII CATG 4 cut(s) 68, 194, 203, 281
CviJI RGCY 3 cut(s) 103, 191, 225
CviKI_1 RGCY 3 cut(s) 103, 191, 225
CviQI GTAC 1 cut(s) 152
DdeI CTNAG 2 cut(s) 90, 99
Eco31I GGTCTC 1 cut(s) 128
EcoRII CCWGG 2 cut(s) 225, 338
FaeI CATG 4 cut(s) 71, 197, 206, 284
FalI AAGNNNNNCTT 4 cut(s) 233, 265, 313, 345
FatI CATG 4 cut(s) 67, 193, 202, 280
Fnu4HI GCNGC 3 cut(s) 134, 200, 223
FokI GGATG 2 cut(s) 32, 245
Fsp4HI GCNGC 3 cut(s) 134, 200, 223
FspBI CTAG 1 cut(s) 317
GluI GCNGC 3 cut(s) 134, 200, 223
Hin1II CATG 4 cut(s) 71, 197, 206, 284
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
Hpy166II GTNNAC 1 cut(s) 108
Hpy188III TCNNGA 1 cut(s) 170
Hpy8I GTNNAC 1 cut(s) 108
HpyAV CCTTC 1 cut(s) 113
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 3 cut(s) 62, 136, 199
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 100, 326
HpyF3I CTNAG 2 cut(s) 90, 99
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 4 cut(s) 71, 197, 206, 284
LmnI GCTCC 1 cut(s) 289
LpnPI CCDG 5 cut(s) 140, 212, 239, 325, 352
Lsp1109I GCAGC 3 cut(s) 120, 211, 234
MaeI CTAG 1 cut(s) 317
MaeII ACGT 1 cut(s) 147
MhlI GDGCHC 1 cut(s) 55
MluCI AATT 1 cut(s) 43
MnlI CCTC 2 cut(s) 10, 166
MseI TTAA 2 cut(s) 114, 251
MspCI CTTAAG 1 cut(s) 250
MspR9I CCNGG 2 cut(s) 227, 340
Mva1269I GAATGC 1 cut(s) 217
MvaI CCWGG 2 cut(s) 227, 340
MwoI GCNNNNNNNGC 3 cut(s) 59, 100, 326
NlaIII CATG 4 cut(s) 71, 197, 206, 284
PctI GAATGC 1 cut(s) 217
PkrI GCNGC 3 cut(s) 135, 201, 224
Psp6I CCWGG 2 cut(s) 225, 338
PspGI CCWGG 2 cut(s) 225, 338
PstI CTGCAG 1 cut(s) 138
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
SaqAI TTAA 2 cut(s) 114, 251
SatI GCNGC 3 cut(s) 134, 200, 223
ScrFI CCNGG 2 cut(s) 227, 340
SduI GDGCHC 1 cut(s) 55
SetI ASST 6 cut(s) 105, 124, 150, 182, 193, 257
SfcI CTRYAG 1 cut(s) 134
SmlI CTYRAG 2 cut(s) 170, 250
SmoI CTYRAG 2 cut(s) 170, 250
Sse9I AATT 1 cut(s) 43
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 2 cut(s) 225, 338
TaaI ACNGT 1 cut(s) 167
TaiI ACGT 1 cut(s) 150
TaqI TCGA 1 cut(s) 49
TasI AATT 1 cut(s) 43
Tru1I TTAA 2 cut(s) 114, 251
Tru9I TTAA 2 cut(s) 114, 251
TscAI CASTG 1 cut(s) 103
TseI GCWGC 3 cut(s) 133, 199, 222
TspDTI ATGAA 2 cut(s) 56, 348
TspRI CASTG 1 cut(s) 103
Vha464I CTTAAG 1 cut(s) 250
XapI RAATTY 1 cut(s) 43
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.