Rorug02G0322400

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017760
Physical Location & Seq
Forward (+)
39451917 .. 39452893
977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0322400.1

Sequence Viewer

Length: 438 bp
ATGGCGGCTCCCCCGATGAAATCGGTGCAGTGCTTCGGGCGCAAGAAGACGGCGGTGGCGGTGACGTACTGCAAGGAAGGGCGGGGGTTGATAAAGATCAACGGGTGCCCAATCGAGCTGGTGGAGCCGGAGATCCTCCGGTTCAAGGCCTACGAGCCGATACTCCTGCTGGGACGACACCGTTTCGCGGGCGTTGACATGAGGATCCGCGTCAAGGGAGGAGGTCACACTTCACAGATCTACGCGATCCGTCAGAGCATCGCCAAGGCGTTGGTGGCTTATTACCAGAAGTTTGTGGATGAGCAGAGCAAGAAGGAGATCAAGGACATTCTGGTCAGGTACGACCGTACTCTCCTTGTGGCTGATCCAAGGCGCTGCGAGCCCAAGAAGTTTGGTGGTCGTGGTGCCAGAGCTAGGTTCCAGAAGTCTTACCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

145

Amino Acids

16.53

Weight (kDa)

10.16

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S9 PF00380 13 - 145 4.3e-38 Ribosomal protein S9/S16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 332
AccB1I GGYRCC 2 cut(s) 105, 404
AccII CGCG 3 cut(s) 188, 210, 245
AciI CCGC 6 cut(s) 5, 53, 59, 82, 188, 208
AclWI GGATC 5 cut(s) 127, 199, 212, 241, 359
AfaI GTAC 3 cut(s) 68, 341, 349
AfiI CCNNNNNNNGG 4 cut(s) 145, 187, 214, 414
AgsI TTSAA 1 cut(s) 145
AluBI AGCT 2 cut(s) 118, 413
AluI AGCT 2 cut(s) 118, 413
AlwI GGATC 5 cut(s) 127, 199, 212, 241, 359
AoxI GGCC 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 375
AspLEI GCGC 2 cut(s) 42, 375
AsuHPI GGTGA 1 cut(s) 73
BaeGI GKGCMC 1 cut(s) 110
BaeI ACNNNNGTAYC 2 cut(s) 331, 364
BamHI GGATCC 1 cut(s) 204
BanI GGYRCC 2 cut(s) 105, 404
BanII GRGCYC 1 cut(s) 384
BbsI GAAGAC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 362
BceAI ACGGC 1 cut(s) 66
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BfaI CTAG 1 cut(s) 414
BfoI RGCGCY 1 cut(s) 376
BglII AGATCT 1 cut(s) 237
BisI GCNGC 2 cut(s) 6, 376
BlsI GCNGC 2 cut(s) 7, 377
BmiI GGNNCC 6 cut(s) 9, 107, 126, 206, 406, 419
BmsI GCATC 1 cut(s) 267
BpiI GAAGAC 1 cut(s) 53
BsaBI GATNNNNATC 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 264, 368
BsaWI WCCGGW 1 cut(s) 138
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 187, 214, 414
Bse8I GATNNNNATC 1 cut(s) 95
BseDI CCNNGG 2 cut(s) 264, 368
BseGI GGATG 1 cut(s) 304
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 4 cut(s) 145, 187, 214, 414
BseRI GAGGAG 1 cut(s) 234
BseSI GKGCMC 1 cut(s) 110
BseXI GCAGC 1 cut(s) 362
BseYI CCCAGC 1 cut(s) 169
BsgI GTGCAG 1 cut(s) 47
Bsh1236I CGCG 3 cut(s) 188, 210, 245
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 1 cut(s) 149
BshNI GGYRCC 2 cut(s) 105, 404
BsiEI CGRYCG 1 cut(s) 346
BsiSI CCGG 2 cut(s) 128, 139
BslFI GGGAC 1 cut(s) 186
BslI CCNNNNNNNGG 4 cut(s) 145, 187, 214, 414
BsmFI GGGAC 1 cut(s) 186
BsnI GGCC 1 cut(s) 149
Bsp1286I GDGCHC 2 cut(s) 110, 384
Bsp143I GATC 7 cut(s) 96, 132, 204, 237, 246, 318, 364
BspACI CCGC 6 cut(s) 5, 53, 59, 82, 188, 208
BspANI GGCC 1 cut(s) 149
BspFNI CGCG 3 cut(s) 188, 210, 245
BspLI GGNNCC 6 cut(s) 9, 107, 126, 206, 406, 419
BspPI GGATC 5 cut(s) 127, 199, 212, 241, 359
BspT107I GGYRCC 2 cut(s) 105, 404
BssECI CCNNGG 2 cut(s) 264, 368
BssMI GATC 7 cut(s) 96, 132, 204, 237, 246, 318, 364
BssT1I CCWWGG 2 cut(s) 264, 368
Bst4CI ACNGT 3 cut(s) 182, 347, 434
BstC8I GCNNGC 2 cut(s) 190, 380
BstF5I GGATG 1 cut(s) 304
BstFNI CGCG 3 cut(s) 188, 210, 245
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 2 cut(s) 42, 375
BstKTI GATC 7 cut(s) 99, 135, 207, 240, 249, 321, 367
BstMBI GATC 7 cut(s) 96, 132, 204, 237, 246, 318, 364
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 4 cut(s) 39, 124, 275, 379
BstSLI GKGCMC 1 cut(s) 110
BstUI CGCG 3 cut(s) 188, 210, 245
BstV1I GCAGC 1 cut(s) 362
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 3 cut(s) 132, 204, 237
BstXI CCANNNNNNTGG 1 cut(s) 271
BstYI RGATCY 3 cut(s) 132, 204, 237
BsuRI GGCC 1 cut(s) 149
BtgZI GCGATG 1 cut(s) 244
BtsCI GGATG 1 cut(s) 304
BtsI GCAGTG 1 cut(s) 35
BtsIMutI CAGTG 1 cut(s) 35
Cac8I GCNNGC 2 cut(s) 190, 380
CfoI GCGC 2 cut(s) 42, 375
CseI GACGC 1 cut(s) 199
Csp6I GTAC 3 cut(s) 67, 340, 348
CviAII CATG 1 cut(s) 199
CviJI RGCY 9 cut(s) 8, 118, 127, 149, 157, 278, 362, 382, 413
CviKI_1 RGCY 9 cut(s) 8, 118, 127, 149, 157, 278, 362, 382, 413
CviQI GTAC 3 cut(s) 67, 340, 348
DpnI GATC 7 cut(s) 98, 134, 206, 239, 248, 320, 366
DpnII GATC 7 cut(s) 96, 132, 204, 237, 246, 318, 364
DrdI GACNNNNNNGTC 1 cut(s) 332
DseDI GACNNNNNNGTC 1 cut(s) 332
Eco130I CCWWGG 2 cut(s) 264, 368
Eco147I AGGCCT 1 cut(s) 149
Eco24I GRGCYC 1 cut(s) 384
EcoT14I CCWWGG 2 cut(s) 264, 368
EcoT38I GRGCYC 1 cut(s) 384
ErhI CCWWGG 2 cut(s) 264, 368
FaeI CATG 1 cut(s) 202
FaiI YATR 1 cut(s) 200
FaqI GGGAC 1 cut(s) 186
FatI CATG 1 cut(s) 198
FauI CCCGC 2 cut(s) 75, 181
Fnu4HI GCNGC 2 cut(s) 6, 376
FokI GGATG 1 cut(s) 311
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 2 cut(s) 6, 376
FspBI CTAG 1 cut(s) 414
GlaI GCGC 2 cut(s) 41, 374
GluI GCNGC 2 cut(s) 6, 376
GsaI CCCAGC 1 cut(s) 173
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 1 cut(s) 149
HapII CCGG 2 cut(s) 128, 139
HgaI GACGC 1 cut(s) 199
HhaI GCGC 2 cut(s) 42, 375
Hin1II CATG 1 cut(s) 202
Hin6I GCGC 2 cut(s) 40, 373
HinP1I GCGC 2 cut(s) 40, 373
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HpaII CCGG 2 cut(s) 128, 139
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 1 cut(s) 255
Hpy188III TCNNGA 1 cut(s) 421
Hpy8I GTNNAC 1 cut(s) 196
HpyAV CCTTC 2 cut(s) 71, 307
HpyCH4III ACNGT 3 cut(s) 182, 347, 434
HpyCH4IV ACGT 1 cut(s) 65
HpyCH4V TGCA 2 cut(s) 28, 72
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 124, 275, 379
HpySE526I ACGT 1 cut(s) 65
Hsp92II CATG 1 cut(s) 202
HspAI GCGC 2 cut(s) 40, 373
Kzo9I GATC 7 cut(s) 96, 132, 204, 237, 246, 318, 364
LmnI GCTCC 2 cut(s) 13, 124
Lsp1109I GCAGC 1 cut(s) 362
LweI GCATC 1 cut(s) 267
MaeI CTAG 1 cut(s) 414
MaeII ACGT 1 cut(s) 65
MaeIII GTNAC 2 cut(s) 61, 224
MalI GATC 7 cut(s) 98, 134, 206, 239, 248, 320, 366
MboI GATC 7 cut(s) 96, 132, 204, 237, 246, 318, 364
MboII GAAGA 1 cut(s) 58
MflI RGATCY 3 cut(s) 132, 204, 237
MhlI GDGCHC 2 cut(s) 110, 384
MnlI CCTC 4 cut(s) 146, 195, 212, 215
MspI CCGG 2 cut(s) 128, 139
MvnI CGCG 3 cut(s) 188, 210, 245
MwoI GCNNNNNNNGC 4 cut(s) 39, 124, 275, 379
NdeII GATC 7 cut(s) 96, 132, 204, 237, 246, 318, 364
NlaIII CATG 1 cut(s) 202
NlaIV GGNNCC 6 cut(s) 9, 107, 126, 206, 406, 419
NmuCI GTSAC 2 cut(s) 61, 224
PceI AGGCCT 1 cut(s) 149
PkrI GCNGC 2 cut(s) 7, 377
PspFI CCCAGC 1 cut(s) 169
PspN4I GGNNCC 6 cut(s) 9, 107, 126, 206, 406, 419
PsuI RGATCY 3 cut(s) 132, 204, 237
RsaI GTAC 3 cut(s) 68, 341, 349
RsaNI GTAC 3 cut(s) 67, 340, 348
SatI GCNGC 2 cut(s) 6, 376
Sau3AI GATC 7 cut(s) 96, 132, 204, 237, 246, 318, 364
SduI GDGCHC 2 cut(s) 110, 384
SetI ASST 6 cut(s) 68, 120, 226, 341, 415, 419
SfaNI GCATC 1 cut(s) 267
SseBI AGGCCT 1 cut(s) 149
SsiI CCGC 6 cut(s) 5, 53, 59, 82, 188, 208
SspMI CTAG 1 cut(s) 414
StuI AGGCCT 1 cut(s) 149
StyI CCWWGG 2 cut(s) 264, 368
TaaI ACNGT 3 cut(s) 182, 347, 434
TaiI ACGT 1 cut(s) 68
TaqI TCGA 1 cut(s) 114
TauI GCSGC 1 cut(s) 8
TscAI CASTG 1 cut(s) 35
TseFI GTSAC 2 cut(s) 61, 224
TseI GCWGC 1 cut(s) 375
Tsp45I GTSAC 2 cut(s) 61, 224
TspDTI ATGAA 1 cut(s) 32
TspGWI ACGGA 1 cut(s) 239
TspRI CASTG 1 cut(s) 35
XcmI CCANNNNNNNNNTGG 1 cut(s) 271
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.