Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_ssc0000314.1

Orthogroup ID

OG0014891

Physical Location & Seq

Genomic Coordinates Reverse (-)

62591 .. 66417

Gene Structure 3827 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3531 bp

atggtgagcagcaagaattcaccttatgattgtggccagttgatagttgacatcaccactaccacttttaccagagcgaagaagaggtggcgcatggcttatgcagccttgtatgctgtaagggtgatgctatcacttcccaaagagcttatgtacaagaggagcaactacaaggaatactcagagatctcggagtacggatctttcggtggtttccaaggtctcaaagaaagccagcgggagaagggagattcgaactctggtctcgagcatgaggctaaaaactcctcaccactggagctacaatcccctgcacatgatgagtctcgttcttacacttccctggaggtgaaaccaagcacttcccctgatgaaacaggtcactatagttctgtccccaacaacattgatcatgcagggctcgaaaaaatggtgaaggaaaaagactcggctgctcttgtaaagtttggaggagttactggcataaccacttctcttaaaacaaatcctgagtatgggctacctggcaacgacgcagaaatcaacaaacgccgtgaactattcggctcaaatacatacccaaagccgccttcaaaagggctcctctattttgtgctggacgctctgaaagacatgacgattctgattctatgtgtctgtgcagcactttctcttgcatttggaatcaaagagtatggagtagaggaaggctggtatgaaggtggaagtatctttgtagctgtaatcattgtcattgttgtctcagctctcagtaacttcagacaggaaatccaattcgacaaactgtctaaaataagcagcaacatcaacgttgaggtatttagagacagacaaagacgagaaatctccatctttgatgttgtggttggagatgtcgtcttccttaacattggagatcaaattcctgcagacggggtgttcttagatggctattcatttcaagtggacgagtccagcatgaccggggagagcgactatgtagaagttgattccagaaggcatcccttcctcttgtctggtgcaaaggtggctgatggttatggtagaatgcttgtcacatcagttgggatgaacactgcttggggcgaaatgatgagctcaatttcgcaggaatcaaatgtaagaactccattacaagctcggctggacaaacttacctcttctattggaaaggtaggccttgctgttgctttcttagttcttctagtcttgttgattcgttatttcactggcaacacaaaagataagaatggcaataaggagtatattggtagcaaaacaaaaacagatgatgtgttgaatgcagtagtacggattgtatctgctgcagtcactattgtggtggtggcaatccctgaaggcttgccactggccgtcacactaacacttgcttactctatgaaaagaatgatgaaagatcaggcaatggttagaaaactatcggcttgtgagacaatggggtcagcaaccgttatctgtactgacaaaacaggaactctgacactaaaccaaatgaaagtaactaagttttggataggccaagaatctattgcagaagatcattcatccatcacaccaaacgttcgtgagttattctatcaaggagttggtttgaacacaactgggagtgttttcatgcctcttagaggatccaaccccgaaatctctggcagcccaacagaaaaggcaatactttattgggttgttttagatttgggtatggacatgcaagaactgaagcccaggtatgacattcttcatgttgaaacatttaattcagacaagaaacgaagtggggttcttataaagaaaaaagatgacaacaccatccatgtgcactggaaaggagctgctgagatgatagttgcaacatgctggagttattatgataccaatggaactgtaaagcccttggatgtagatacaaggtctaaacttgacaaaataatccaaggcatggcagctagtagcctccgatgcattgctttcgctcatgcccaaatttcagaggaagaagttgaatacagttatgatgacaagacacaccaaaagctcaaagaagatgggctgatcttgctagggatagttggcctcaaagatccatgtcgaccaggggtcatcggtgctgtggaagcttgcaaggctgcaggtgtggacatcaaaatgataactggagacaatattttcacagcaaaagctatagctacagaatgtggaatactaaagaatgagaacgaggtaaaggatggacaagttgtagaaggagtcgaatttcgaaactacactgacacagagagaatgcagaaggttgataacatccgtgtgatggcaaggtcctccccatttgacaagcttttaatggtacagtgtttgaaggagaaaggtcatgtggttgcagtcactggtgatggtacgaatgatgcacctgcattgaaagaagctgatataggactttccatgggcatccaaggaactgaggtggcaaaagaaagctcggatatcatcatcctggatgacaacttcaattctgtggccacagttttgagatgggggagatgtgtttataacaatatacagaaattcatccaatttcaactaactgttaatgttgcagctctagtaatcaactttattgcagcagtttcagctggtgaagttcccctaacagcagttcagttactttgggtgaatctcatcatggacacgctaggtgctctggctcttgctacggagcggccaactaatgagctaatggagaggcagcctgtgggtcggaccgctccactcataaccaatattatgtggaggaatcttttagctcaagctctatatcaaatagctgttctcttgatcttgcagttccagggtgagtctatattcaatgtcactgctgcggtgaatgatactctgattttcaatacctttgtattatgccaagttttcaacgagttcaattcaagaagcatggagaagaagaatgtattcaaaggtatcctcaggaacaggctgtttattgggattgtggggataactattgttcttcaggttgtgatggtggagtttctgaagaaatttgcagatacagaaaagttgaatgttcagcaatgggtagtttgtatcatgattgcagctgtatcatggccaattggggttgcaatccagaattgtcttgccatcaacacacacacgccacgaaccttctttttcgggagtaagcaagccagagctcccggcccgacctcaaggcttggacccgaatctcaagttctgggtccattgattgtggcctggggttatttggcactgaaagtcaggagattgaagcttgcctctggtcggagtctgggcactggccccaaaccccgagctgcgacaactagacacgcggacttggatttgactctcaatatagatctcacatccaagtccgagtgtccggtgtttggggctcatggacaccgccatgagcttgaaattttaagcttgagtgaatttgtaggattggaataa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1176

Amino Acids

129.48

Weight (kDa)

6.45

Isoelectric Point (pI)

34.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	157 - 224	1.6e-12	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	277 - 375	3.6e-21	P-type ATPase actuator domain
Cation_ATPase	PF13246	559 - 711	2.4e-29	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	681 - 822	2.7e-12	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	893 - 1057	8.1e-45	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014891)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63380
fragaria_vesca	FvH4_2g22900
malus_domestica	MD11G1278100.v1.1
prunus_persica	Prupe.8G234200_v2.0.a1 Prupe.8G234200_v2.0.a1
pyrus_communis	pycom11g24580
rosa_chinensis	RchiOBHm_Chr6g0289831
rosa_laevigata	RLG00000012264
rosa_multiflora	Rmu_sc0032573.1_g000001 Rmu_ssc0000314.1_g000015
rosa_roxburghii	Rroxscaffold_4G00321460 Rroxscaffold_7G00177520 Rroxscaffold_7G00178050
rosa_rugosa	Rorug06G0207300
rosa_samantha	Rh6AG318800
rosa_wichuraiana	Rw6G027500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1826, 2586
AarI	CACCTGC	2 cut(s)	2159, 2455
AasI	GACNNNNNNGTC	1 cut(s)	1480
Acc36I	ACCTGC	2 cut(s)	2159, 2455
AccB7I	CCANNNNNTGG	1 cut(s)	1978
AccBSI	CCGCTC	2 cut(s)	2755, 2801
AccI	GTMKAC	1 cut(s)	2128
AccII	CGCG	1 cut(s)	3407
AciI	CCGC	7 cut(s)	238, 587, 2755, 2799, 2915, 3407, 3481
AclI	AACGTT	2 cut(s)	831, 1602
AclWI	GGATC	4 cut(s)	208, 1665, 1678, 2114
AcoI	YGGCCR	5 cut(s)	34, 1392, 2553, 2756, 3161
AcsI	RAATTY	8 cut(s)	16, 921, 2022, 2291, 2600, 3092, 3495, 3512
AcuI	CTGAAG	5 cut(s)	763, 1398, 1778, 3047, 3107
AfaI	GTAC	6 cut(s)	155, 197, 1332, 1501, 2385, 2434
AfiI	CCNNNNNNNGG	8 cut(s)	515, 596, 932, 1667, 1978, 2347, 3357, 3464
AhdI	GACNNNNNGTC	2 cut(s)	805, 2135
AjnI	CCWGG	7 cut(s)	342, 523, 1763, 2131, 2529, 2883, 3308
AjuI	GAANNNNNNNTTGG	2 cut(s)	574, 606
AleI	CACNNNNGTG	1 cut(s)	1358
Alw21I	GWGCWC	4 cut(s)	1121, 1860, 2737, 3250
Alw26I	GTCTC	7 cut(s)	227, 269, 330, 766, 840, 1466, 2190
Alw44I	GTGCAC	1 cut(s)	1856
AlwI	GGATC	4 cut(s)	208, 1665, 1678, 2114
AlwNI	CAGNNNCTG	1 cut(s)	2423
Ama87I	CYCGRG	2 cut(s)	266, 3384
ApaLI	GTGCAC	1 cut(s)	1856
ApoI	RAATTY	8 cut(s)	16, 921, 2022, 2291, 2600, 3092, 3495, 3512
Asp700I	GAANNNNTTC	1 cut(s)	3002
AspLEI	GCGC	1 cut(s)	93
AspS9I	GGNCC	6 cut(s)	2355, 2796, 3254, 3272, 3293, 3374
AsuC2I	CCSGG	2 cut(s)	985, 3252
AsuII	TTCGAA	2 cut(s)	254, 2296
AvaI	CYCGRG	2 cut(s)	266, 3384
AvaII	GGWCC	4 cut(s)	2355, 2796, 3272, 3293
AxyI	CCTNAGG	1 cut(s)	3017
BaeGI	GKGCMC	2 cut(s)	1860, 3371
BalI	TGGCCA	3 cut(s)	36, 2555, 3163
BamHI	GGATCC	1 cut(s)	1670
BanII	GRGCYC	5 cut(s)	423, 603, 1121, 3250, 3472
BbsI	GAAGAC	1 cut(s)	892
Bbv12I	GWGCWC	4 cut(s)	1121, 1860, 2737, 3250
BceAI	ACGGC	2 cut(s)	537, 1379
BcgI	CGANNNNNNTGC	2 cut(s)	1990, 2024
BciT130I	CCWGG	7 cut(s)	344, 525, 1765, 2133, 2531, 2885, 3310
BciVI	GTATCC	1 cut(s)	3023
BclI	TGATCA	1 cut(s)	409
BcnI	CCSGG	2 cut(s)	985, 3252
BcoDI	GTCTC	7 cut(s)	227, 269, 330, 766, 840, 1466, 2190
BfaI	CTAG	6 cut(s)	1226, 1986, 2099, 2639, 2729, 3399
BfmI	CTRYAG	6 cut(s)	385, 927, 1347, 2166, 2220, 2226
BfuAI	ACCTGC	2 cut(s)	2159, 2455
BfuI	GTATCC	1 cut(s)	3023
BglII	AGATCT	2 cut(s)	186, 3433
Bme1390I	CCNGG	9 cut(s)	344, 525, 985, 1765, 2133, 2531, 2885, 3252, 3310
Bme18I	GGWCC	4 cut(s)	2355, 2796, 3272, 3293
BmeRI	GACNNNNNGTC	2 cut(s)	805, 2135
BmeT110I	CYCGRG	2 cut(s)	266, 3384
BmgT120I	GGNCC	6 cut(s)	2355, 2796, 3254, 3272, 3293, 3374
BmiI	GGNNCC	5 cut(s)	602, 1672, 3274, 3294, 3376
BmrFI	CCNGG	9 cut(s)	344, 525, 985, 1765, 2133, 2531, 2885, 3252, 3310
BmrI	ACTGGG	1 cut(s)	1653
BmsI	GCATC	5 cut(s)	117, 1030, 1988, 2431, 2493
BmuI	ACTGGG	1 cut(s)	1653
BpiI	GAAGAC	1 cut(s)	892
BpmI	CTGGAG	4 cut(s)	317, 365, 1918, 2214
Bpu14I	TTCGAA	2 cut(s)	254, 2296
BpuEI	CTTGAG	4 cut(s)	2826, 3247, 3267, 3526
BpuMI	CCSGG	2 cut(s)	985, 3252
BsaI	GGTCTC	2 cut(s)	227, 269
BsaWI	WCCGGW	1 cut(s)	3457
BsaXI	ACNNNNNCTCC	2 cut(s)	882, 912
Bsc4I	CCNNNNNNNGG	8 cut(s)	515, 596, 932, 1667, 1978, 2347, 3357, 3464
Bse21I	CCTNAGG	1 cut(s)	3017
Bse3DI	GCAATG	3 cut(s)	1452, 2001, 3131
BseBI	CCWGG	7 cut(s)	344, 525, 1765, 2133, 2531, 2885, 3310
BseLI	CCNNNNNNNGG	8 cut(s)	515, 596, 932, 1667, 1978, 2347, 3357, 3464
BseMI	GCAATG	3 cut(s)	1452, 2001, 3131
BseMII	CTCAG	7 cut(s)	195, 501, 777, 784, 1866, 2487, 3031
BseRI	GAGGAG	4 cut(s)	175, 277, 486, 593
BseSI	GKGCMC	2 cut(s)	1860, 3371
BsgI	GTGCAG	2 cut(s)	297, 681
Bsh1236I	CGCG	1 cut(s)	3407
BsiHKAI	GWGCWC	4 cut(s)	1121, 1860, 2737, 3250
BsiHKCI	CYCGRG	2 cut(s)	266, 3384
BsiSI	CCGG	3 cut(s)	984, 3252, 3458
BslFI	GGGAC	1 cut(s)	380
BslI	CCNNNNNNNGG	8 cut(s)	515, 596, 932, 1667, 1978, 2347, 3357, 3464
BsmAI	GTCTC	7 cut(s)	227, 269, 330, 766, 840, 1466, 2190
BsmFI	GGGAC	1 cut(s)	380
BsmI	GAATGC	3 cut(s)	1074, 1327, 2325
Bso31I	GGTCTC	2 cut(s)	227, 269
BsoBI	CYCGRG	2 cut(s)	266, 3384
Bsp119I	TTCGAA	2 cut(s)	254, 2296
Bsp1286I	GDGCHC	8 cut(s)	423, 603, 1121, 1860, 2737, 3250, 3371, 3472
Bsp1407I	TGTACA	1 cut(s)	153
Bsp19I	CCATGG	1 cut(s)	2478
BspACI	CCGC	7 cut(s)	238, 587, 2755, 2799, 2915, 3407, 3481
BspCNI	CTCAG	7 cut(s)	194, 502, 776, 783, 1867, 2488, 3030
BspFNI	CGCG	1 cut(s)	3407
BspHI	TCATGA	1 cut(s)	3141
BspLI	GGNNCC	5 cut(s)	602, 1672, 3274, 3294, 3376
BspMAI	CTGCAG	3 cut(s)	931, 1351, 2170
BspMI	ACCTGC	2 cut(s)	2159, 2455
BspPI	GGATC	4 cut(s)	208, 1665, 1678, 2114
BspT104I	TTCGAA	2 cut(s)	254, 2296
BspTNI	GGTCTC	2 cut(s)	227, 269
BsrBI	CCGCTC	2 cut(s)	2755, 2801
BsrDI	GCAATG	3 cut(s)	1452, 2001, 3131
BsrGI	TGTACA	1 cut(s)	153
BssT1I	CCWWGG	5 cut(s)	217, 1932, 1972, 2478, 2488
Bst2UI	CCWGG	7 cut(s)	344, 525, 1765, 2133, 2531, 2885, 3310
Bst4CI	ACNGT	7 cut(s)	807, 1492, 1924, 2048, 2388, 2560, 2623
Bst6I	CTCTTC	2 cut(s)	77, 1186
BstAUI	TGTACA	1 cut(s)	153
BstBI	TTCGAA	2 cut(s)	254, 2296
BstC8I	GCNNGC	5 cut(s)	236, 1385, 2158, 3240, 3348
BstDSI	CCRYGG	1 cut(s)	2478
BstENI	CCTNNNNNAGG	2 cut(s)	594, 1665
BstFNI	CGCG	1 cut(s)	3407
BstHHI	GCGC	1 cut(s)	93
BstMAI	GTCTC	7 cut(s)	227, 269, 330, 766, 840, 1466, 2190
BstMWI	GCNNNNNNNGC	8 cut(s)	104, 113, 1049, 1998, 2095, 2153, 2162, 2666
BstNI	CCWGG	7 cut(s)	344, 525, 1765, 2133, 2531, 2885, 3310
BstNSI	RCATGY	2 cut(s)	1750, 1896
BstSCI	CCNGG	9 cut(s)	342, 523, 983, 1763, 2131, 2529, 2883, 3250, 3308
BstSFI	CTRYAG	6 cut(s)	385, 927, 1347, 2166, 2220, 2226
BstSLI	GKGCMC	2 cut(s)	1860, 3371
BstUI	CGCG	1 cut(s)	3407
BstV2I	GAAGAC	1 cut(s)	892
BstX2I	RGATCY	5 cut(s)	186, 200, 1670, 2119, 3433
BstYI	RGATCY	5 cut(s)	186, 200, 1670, 2119, 3433
Bsu36I	CCTNAGG	1 cut(s)	3017
BsuI	GTATCC	1 cut(s)	3023
BtgI	CCRYGG	1 cut(s)	2478
BtsI	GCAGTG	2 cut(s)	1095, 2907
BveI	ACCTGC	2 cut(s)	2159, 2455
Cac8I	GCNNGC	5 cut(s)	236, 1385, 2158, 3240, 3348
CaiI	CAGNNNCTG	1 cut(s)	2423
CciI	TCATGA	1 cut(s)	3141
CfoI	GCGC	1 cut(s)	93
Cfr13I	GGNCC	6 cut(s)	2355, 2796, 3254, 3272, 3293, 3374
CpoI	CGGWCCG	1 cut(s)	2796
CseI	GACGC	2 cut(s)	542, 629
Csp6I	GTAC	6 cut(s)	154, 196, 1331, 1500, 2384, 2433
CspI	CGGWCCG	1 cut(s)	2796
CviQI	GTAC	6 cut(s)	154, 196, 1331, 1500, 2384, 2433
DrdI	GACNNNNNNGTC	1 cut(s)	1480
DriI	GACNNNNNGTC	2 cut(s)	805, 2135
DseDI	GACNNNNNNGTC	1 cut(s)	1480
EaeI	YGGCCR	5 cut(s)	34, 1392, 2553, 2756, 3161
Eam1104I	CTCTTC	2 cut(s)	77, 1186
Eam1105I	GACNNNNNGTC	2 cut(s)	805, 2135
EarI	CTCTTC	2 cut(s)	77, 1186
Ecl136II	GAGCTC	2 cut(s)	1119, 3248
Eco130I	CCWWGG	5 cut(s)	217, 1932, 1972, 2478, 2488
Eco147I	AGGCCT	1 cut(s)	1200
Eco24I	GRGCYC	5 cut(s)	423, 603, 1121, 3250, 3472
Eco31I	GGTCTC	2 cut(s)	227, 269
Eco32I	GATATC	1 cut(s)	2521
Eco47I	GGWCC	4 cut(s)	2355, 2796, 3272, 3293
Eco53kI	GAGCTC	2 cut(s)	1119, 3248
Eco57I	CTGAAG	5 cut(s)	763, 1398, 1778, 3047, 3107
Eco81I	CCTNAGG	1 cut(s)	3017
Eco88I	CYCGRG	2 cut(s)	266, 3384
EcoICRI	GAGCTC	2 cut(s)	1119, 3248
EcoNI	CCTNNNNNAGG	2 cut(s)	594, 1665
EcoO109I	RGGNCCY	1 cut(s)	2355
EcoRI	GAATTC	1 cut(s)	16
EcoRII	CCWGG	7 cut(s)	342, 523, 1763, 2131, 2529, 2883, 3308
EcoRV	GATATC	1 cut(s)	2521
EcoT14I	CCWWGG	5 cut(s)	217, 1932, 1972, 2478, 2488
EcoT22I	ATGCAT	1 cut(s)	2003
EcoT38I	GRGCYC	5 cut(s)	423, 603, 1121, 3250, 3472
ErhI	CCWWGG	5 cut(s)	217, 1932, 1972, 2478, 2488
FaqI	GGGAC	1 cut(s)	380
FauI	CCCGC	1 cut(s)	231
FbaI	TGATCA	1 cut(s)	409
FblI	GTMKAC	1 cut(s)	2128
FriOI	GRGCYC	5 cut(s)	423, 603, 1121, 3250, 3472
FspBI	CTAG	6 cut(s)	1226, 1986, 2099, 2639, 2729, 3399
GlaI	GCGC	1 cut(s)	92
GsuI	CTGGAG	4 cut(s)	317, 365, 1918, 2214
HapII	CCGG	3 cut(s)	984, 3252, 3458
HgaI	GACGC	2 cut(s)	542, 629
HhaI	GCGC	1 cut(s)	93
Hin6I	GCGC	1 cut(s)	91
HinP1I	GCGC	1 cut(s)	91
HincII	GTYRAC	2 cut(s)	49, 2129
HindII	GTYRAC	2 cut(s)	49, 2129
HindIII	AAGCTT	4 cut(s)	2154, 2372, 3344, 3502
HpaII	CCGG	3 cut(s)	984, 3252, 3458
Hpy166II	GTNNAC	6 cut(s)	49, 557, 967, 1858, 2129, 2176
Hpy8I	GTNNAC	6 cut(s)	49, 557, 967, 1858, 2129, 2176
Hpy99I	CGWCG	1 cut(s)	536
HpyCH4III	ACNGT	7 cut(s)	807, 1492, 1924, 2048, 2388, 2560, 2623
HpyCH4IV	ACGT	2 cut(s)	831, 1602
HpyF10VI	GCNNNNNNNGC	8 cut(s)	104, 113, 1049, 1998, 2095, 2153, 2162, 2666
HpySE526I	ACGT	2 cut(s)	831, 1602
HspAI	GCGC	1 cut(s)	91
Ksp22I	TGATCA	1 cut(s)	409
LmnI	GCTCC	7 cut(s)	162, 298, 606, 1868, 2752, 2806, 3253
LweI	GCATC	5 cut(s)	117, 1030, 1988, 2431, 2493
MaeI	CTAG	6 cut(s)	1226, 1986, 2099, 2639, 2729, 3399
MaeII	ACGT	2 cut(s)	831, 1602
MbiI	CCGCTC	2 cut(s)	2755, 2801
MfeI	CAATTG	1 cut(s)	3165
MflI	RGATCY	5 cut(s)	186, 200, 1670, 2119, 3433
MhlI	GDGCHC	8 cut(s)	423, 603, 1121, 1860, 2737, 3250, 3371, 3472
MlsI	TGGCCA	3 cut(s)	36, 2555, 3163
MluNI	TGGCCA	3 cut(s)	36, 2555, 3163
MlyI	GAGTC	7 cut(s)	332, 440, 980, 2295, 2900, 3370, 3415
MmeI	TCCRAC	4 cut(s)	868, 1698, 2774, 3338
Mox20I	TGGCCA	3 cut(s)	36, 2555, 3163
Mph1103I	ATGCAT	1 cut(s)	2003
MroXI	GAANNNNTTC	1 cut(s)	3002
MscI	TGGCCA	3 cut(s)	36, 2555, 3163
MseI	TTAA	6 cut(s)	498, 906, 1794, 2378, 2625, 3500
MslI	CAYNNNNRTG	5 cut(s)	1358, 1853, 2183, 2342, 3483
Msp20I	TGGCCA	3 cut(s)	36, 2555, 3163
MspA1I	CMGCKG	3 cut(s)	238, 2669, 3152
MspI	CCGG	3 cut(s)	984, 3252, 3458
MspR9I	CCNGG	9 cut(s)	344, 525, 985, 1765, 2133, 2531, 2885, 3252, 3310
MunI	CAATTG	1 cut(s)	3165
Mva1269I	GAATGC	3 cut(s)	1074, 1327, 2325
MvaI	CCWGG	7 cut(s)	344, 525, 1765, 2133, 2531, 2885, 3310
MvnI	CGCG	1 cut(s)	3407
MwoI	GCNNNNNNNGC	8 cut(s)	104, 113, 1049, 1998, 2095, 2153, 2162, 2666
NciI	CCSGG	2 cut(s)	985, 3252
NcoI	CCATGG	1 cut(s)	2478
NlaIV	GGNNCC	5 cut(s)	602, 1672, 3274, 3294, 3376
NmeAIII	GCCGAG	2 cut(s)	428, 1141
NmuCI	GTSAC	6 cut(s)	380, 1075, 1351, 1396, 2419, 2905
NsiI	ATGCAT	1 cut(s)	2003
NspI	RCATGY	2 cut(s)	1750, 1896
NspV	TTCGAA	2 cut(s)	254, 2296
OliI	CACNNNNGTG	1 cut(s)	1358
PaeR7I	CTCGAG	1 cut(s)	266
PagI	TCATGA	1 cut(s)	3141
PaqCI	CACCTGC	2 cut(s)	2159, 2455
PceI	AGGCCT	1 cut(s)	1200
PctI	GAATGC	3 cut(s)	1074, 1327, 2325
PdmI	GAANNNNTTC	1 cut(s)	3002
PflMI	CCANNNNNTGG	1 cut(s)	1978
PfoI	TCCNGGA	1 cut(s)	2529
PleI	GAGTC	7 cut(s)	331, 440, 979, 2294, 2899, 3369, 3415
PpsI	GAGTC	7 cut(s)	331, 440, 979, 2294, 2899, 3369, 3415
PpuMI	RGGWCCY	1 cut(s)	2355
PsiI	TTATAA	2 cut(s)	1826, 2586
Psp124BI	GAGCTC	2 cut(s)	1121, 3250
Psp1406I	AACGTT	2 cut(s)	831, 1602
Psp5II	RGGWCCY	1 cut(s)	2355
Psp6I	CCWGG	7 cut(s)	342, 523, 1763, 2131, 2529, 2883, 3308
PspGI	CCWGG	7 cut(s)	342, 523, 1763, 2131, 2529, 2883, 3308
PspN4I	GGNNCC	5 cut(s)	602, 1672, 3274, 3294, 3376
PspPI	GGNCC	6 cut(s)	2355, 2796, 3254, 3272, 3293, 3374
PspPPI	RGGWCCY	1 cut(s)	2355
PstI	CTGCAG	3 cut(s)	931, 1351, 2170
PstNI	CAGNNNCTG	1 cut(s)	2423
PsuI	RGATCY	5 cut(s)	186, 200, 1670, 2119, 3433
PvuII	CAGCTG	2 cut(s)	2669, 3152
RsaI	GTAC	6 cut(s)	155, 197, 1332, 1501, 2385, 2434
RsaNI	GTAC	6 cut(s)	154, 196, 1331, 1500, 2384, 2433
RseI	CAYNNNNRTG	5 cut(s)	1358, 1853, 2183, 2342, 3483
Rsr2I	CGGWCCG	1 cut(s)	2796
RsrII	CGGWCCG	1 cut(s)	2796
SacI	GAGCTC	2 cut(s)	1121, 3250
SalI	GTCGAC	1 cut(s)	2127
SaqAI	TTAA	6 cut(s)	498, 906, 1794, 2378, 2625, 3500
Sau96I	GGNCC	6 cut(s)	2355, 2796, 3254, 3272, 3293, 3374
SchI	GAGTC	7 cut(s)	332, 440, 980, 2295, 2900, 3370, 3415
ScrFI	CCNGG	9 cut(s)	344, 525, 985, 1765, 2133, 2531, 2885, 3252, 3310
SduI	GDGCHC	8 cut(s)	423, 603, 1121, 1860, 2737, 3250, 3371, 3472
SfaNI	GCATC	5 cut(s)	117, 1030, 1988, 2431, 2493
SfcI	CTRYAG	6 cut(s)	385, 927, 1347, 2166, 2220, 2226
Sfr274I	CTCGAG	1 cut(s)	266
SfuI	TTCGAA	2 cut(s)	254, 2296
SinI	GGWCC	4 cut(s)	2355, 2796, 3272, 3293
SlaI	CTCGAG	1 cut(s)	266
SmiMI	CAYNNNNRTG	5 cut(s)	1358, 1853, 2183, 2342, 3483
SmlI	CTYRAG	5 cut(s)	266, 2841, 3262, 3282, 3505
SmoI	CTYRAG	5 cut(s)	266, 2841, 3262, 3282, 3505
SseBI	AGGCCT	1 cut(s)	1200
SsiI	CCGC	7 cut(s)	238, 587, 2755, 2799, 2915, 3407, 3481
SspI	AATATT	2 cut(s)	2203, 2818
SspMI	CTAG	6 cut(s)	1226, 1986, 2099, 2639, 2729, 3399
SstI	GAGCTC	2 cut(s)	1121, 3250
StuI	AGGCCT	1 cut(s)	1200
StyD4I	CCNGG	9 cut(s)	342, 523, 983, 1763, 2131, 2529, 2883, 3250, 3308
StyI	CCWWGG	5 cut(s)	217, 1932, 1972, 2478, 2488
TaaI	ACNGT	7 cut(s)	807, 1492, 1924, 2048, 2388, 2560, 2623
TaiI	ACGT	2 cut(s)	834, 1605
TaqI	TCGA	7 cut(s)	254, 267, 423, 798, 2128, 2289, 2296
TatI	WGTACW	2 cut(s)	153, 1499
TauI	GCSGC	2 cut(s)	589, 2758
Tru1I	TTAA	6 cut(s)	498, 906, 1794, 2378, 2625, 3500
Tru9I	TTAA	6 cut(s)	498, 906, 1794, 2378, 2625, 3500
TseFI	GTSAC	6 cut(s)	380, 1075, 1351, 1396, 2419, 2905
Tsp45I	GTSAC	6 cut(s)	380, 1075, 1351, 1396, 2419, 2905
TspGWI	ACGGA	4 cut(s)	213, 1348, 2330, 2765
Van91I	CCANNNNNTGG	1 cut(s)	1978
VneI	GTGCAC	1 cut(s)	1856
VpaK11BI	GGWCC	4 cut(s)	2355, 2796, 3272, 3293
XagI	CCTNNNNNAGG	2 cut(s)	594, 1665
XapI	RAATTY	8 cut(s)	16, 921, 2022, 2291, 2600, 3092, 3495, 3512
XceI	RCATGY	2 cut(s)	1750, 1896
XhoI	CTCGAG	1 cut(s)	266
XmiI	GTMKAC	1 cut(s)	2128
XmnI	GAANNNNTTC	1 cut(s)	3002
XspI	CTAG	6 cut(s)	1226, 1986, 2099, 2639, 2729, 3399
Zsp2I	ATGCAT	1 cut(s)	2003

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.