Rmu_ssc0000350.1_g000037

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000350.1
OG0012390
Physical Location & Seq
Reverse (-)
211501 .. 215984
4484 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
atgggtaatgcagtgtctccatgttttcaacaaaactcaaactcagctgttaaagtaatcttttcccaaggcaccaccagactcctcagaggaaaacacatcgccggagagatcatgttcgagtttcccgaccaaatggtttgccatgcagactccttcttcatcggtcaacctattccggccttgtccatcgacgacgagcttatgctcggccagacctacttcgttcttcccctcgatcgcttcgcaggcagtgtgctctccgcctcctctctcgcggcgttaaactcgtcgagccctaagaattctccgatcaaattcggggagagccccttcgagtacataaagggttccaatggaaaggtgttgatcaaagttgcgccggagttcattaccaggctgattacaagaggtcaggaaatggagtccgaaagtccaggcaatcgttttctctgtagcacaccggagcttcagaaacactacgagcagcttgttggttctaaagagcaatcgtggtcgcccaagcttgaaaccatttctgagcataaaattaggttttcaccatgcagattcatagggctagagtactggaaaccgaaagagacgcaagtcattccaaacacatcccccgtcttccccagccaccgtagccctatgcctaggctaggctatggggtaggcttctctttttggctctcagcattccaaacacaaaagcaaatcccgattaaatctcctcacgcgccagcccaaccgcagaccgaggaggcgcgtgggacaaaacaaaaagtagggccgcatcgccctcctcctcgacctcctccacaagtccccgttctctggttcgacgccgacaccaccaacaactccggcctctccgtccaggccctatccgaatcctacttcctcggcgccatctccggactctccttcctcttcgtctacgaccccaacgagtcgtcgattccggttatgagatcgagaagttgccgagattgttgcagcagacgagcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

338

Amino Acids

37.53

Weight (kDa)

8.92

Isoelectric Point (pI)

55.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 181 5.3e-42 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012390)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12340 AT5G12340
fragaria_vesca FvH4_3g27940
malus_domestica MD03G1167500.v1.1 MD11G1186400.v1.1
prunus_persica Prupe.4G267600_v2.0.a1
pyrus_communis pycom03g12270 pycom11g16110
rosa_chinensis RchiOBHm_Chr5g0050661
rosa_laevigata RLG00000034716
rosa_multiflora Rmu_ssc0000350.1_g000037 Rmu_ssc0000350.1_g000038
rosa_roxburghii Rroxscaffold_1G00030140
rosa_rugosa Rorug05G0262800
rosa_samantha Rh5AG334800 Rh5BG344100 Rh5CG371900 Rh5DG357200
rosa_wichuraiana Rw5G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 71, 911
AccI GTMKAC 1 cut(s) 942
AccII CGCG 3 cut(s) 278, 743, 772
AccIII TCCGGA 1 cut(s) 920
AciI CCGC 4 cut(s) 264, 278, 755, 797
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 2 cut(s) 304, 317
AcuI CTGAAG 1 cut(s) 455
AcyI GRCGYC 2 cut(s) 849, 912
AfaI GTAC 2 cut(s) 341, 587
AfiI CCNNNNNNNGG 2 cut(s) 665, 840
AgsI TTSAA 2 cut(s) 29, 530
AjnI CCWGG 3 cut(s) 395, 436, 882
AluBI AGCT 5 cut(s) 47, 202, 469, 490, 526
AluI AGCT 5 cut(s) 47, 202, 469, 490, 526
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 21, 596
Aor13HI TCCGGA 1 cut(s) 920
AoxI GGCC 5 cut(s) 180, 211, 794, 871, 885
ApeKI GCWGC 2 cut(s) 487, 1002
ApoI RAATTY 2 cut(s) 304, 317
AspA2I CCTAGG 1 cut(s) 659
AspLEI GCGC 4 cut(s) 382, 745, 772, 914
AspS9I GGNCC 2 cut(s) 794, 886
AsuHPI GGTGA 1 cut(s) 552
AvrII CCTAGG 1 cut(s) 659
BanI GGYRCC 2 cut(s) 71, 911
BanII GRGCYC 2 cut(s) 299, 332
BbsI GAAGAC 1 cut(s) 625
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 1 cut(s) 499
BccI CCATC 2 cut(s) 197, 923
BcgI CGANNNNNNTGC 2 cut(s) 981, 1015
BciT130I CCWGG 3 cut(s) 397, 438, 884
BclI TGATCA 1 cut(s) 369
BcoDI GTCTC 2 cut(s) 21, 596
BfaI CTAG 3 cut(s) 581, 660, 665
BfmI CTRYAG 1 cut(s) 454
BfoI RGCGCY 1 cut(s) 915
BisI GCNGC 4 cut(s) 279, 488, 797, 1003
BlnI CCTAGG 1 cut(s) 659
BlsI GCNGC 4 cut(s) 280, 489, 798, 1004
BmcAI AGTACT 1 cut(s) 587
Bme1390I CCNGG 3 cut(s) 397, 438, 884
BmgT120I GGNCC 2 cut(s) 794, 886
BmiI GGNNCC 3 cut(s) 73, 352, 913
BmrFI CCNGG 3 cut(s) 397, 438, 884
BmsI GCATC 1 cut(s) 808
BoxI GACNNNNGTC 1 cut(s) 608
BpiI GAAGAC 1 cut(s) 625
BsaHI GRCGYC 2 cut(s) 849, 912
BsaJI CCNNGG 4 cut(s) 67, 659, 762, 907
BsaWI WCCGGW 3 cut(s) 463, 920, 967
Bsc4I CCNNNNNNNGG 2 cut(s) 665, 840
Bse1I ACTGG 1 cut(s) 593
BseAI TCCGGA 1 cut(s) 920
BseBI CCWGG 3 cut(s) 397, 438, 884
BseDI CCNNGG 4 cut(s) 67, 659, 762, 907
BseGI GGATG 1 cut(s) 623
BseLI CCNNNNNNNGG 2 cut(s) 665, 840
BseMII CTCAG 4 cut(s) 57, 100, 531, 711
BseNI ACTGG 1 cut(s) 593
BseRI GAGGAG 7 cut(s) 74, 259, 726, 779, 798, 801, 810
BseXI GCAGC 1 cut(s) 499
BseYI CCCAGC 1 cut(s) 638
Bsh1236I CGCG 3 cut(s) 278, 743, 772
Bsh1285I CGRYCG 1 cut(s) 241
BshFI GGCC 5 cut(s) 182, 213, 796, 873, 887
BshNI GGYRCC 2 cut(s) 71, 911
BsiEI CGRYCG 1 cut(s) 241
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 7 cut(s) 105, 179, 383, 464, 870, 921, 968
BslFI GGGAC 2 cut(s) 790, 815
BslI CCNNNNNNNGG 2 cut(s) 665, 840
BsmAI GTCTC 2 cut(s) 21, 596
BsmBI CGTCTC 1 cut(s) 596
BsmFI GGGAC 2 cut(s) 790, 815
BsmI GAATGC 1 cut(s) 701
BsnI GGCC 5 cut(s) 182, 213, 796, 873, 887
Bsp1286I GDGCHC 3 cut(s) 261, 299, 332
Bsp13I TCCGGA 1 cut(s) 920
Bsp143I GATC 5 cut(s) 111, 238, 312, 369, 977
BspACI CCGC 4 cut(s) 264, 278, 755, 797
BspANI GGCC 5 cut(s) 182, 213, 796, 873, 887
BspCNI CTCAG 4 cut(s) 56, 99, 532, 710
BspEI TCCGGA 1 cut(s) 920
BspFNI CGCG 3 cut(s) 278, 743, 772
BspLI GGNNCC 3 cut(s) 73, 352, 913
BspT107I GGYRCC 2 cut(s) 71, 911
BsrI ACTGG 1 cut(s) 593
BssECI CCNNGG 4 cut(s) 67, 659, 762, 907
BssMI GATC 5 cut(s) 111, 238, 312, 369, 977
BssNI GRCGYC 2 cut(s) 849, 912
BssT1I CCWWGG 2 cut(s) 67, 659
Bst2UI CCWGG 3 cut(s) 397, 438, 884
Bst4CI ACNGT 1 cut(s) 647
Bst6I CTCTTC 1 cut(s) 941
BstACI GRCGYC 2 cut(s) 849, 912
BstC8I GCNNGC 2 cut(s) 250, 747
BstDEI CTNAG 5 cut(s) 43, 86, 300, 540, 697
BstENI CCTNNNNNAGG 1 cut(s) 663
BstF5I GGATG 1 cut(s) 623
BstFNI CGCG 3 cut(s) 278, 743, 772
BstH2I RGCGCY 1 cut(s) 915
BstHHI GCGC 4 cut(s) 382, 745, 772, 914
BstKTI GATC 5 cut(s) 114, 241, 315, 372, 980
BstMAI GTCTC 2 cut(s) 21, 596
BstMBI GATC 5 cut(s) 111, 238, 312, 369, 977
BstMCI CGRYCG 1 cut(s) 241
BstMWI GCNNNNNNNGC 2 cut(s) 249, 648
BstNI CCWGG 3 cut(s) 397, 438, 884
BstPAI GACNNNNGTC 1 cut(s) 608
BstSCI CCNGG 3 cut(s) 395, 436, 882
BstSFI CTRYAG 1 cut(s) 454
BstUI CGCG 3 cut(s) 278, 743, 772
BstV1I GCAGC 1 cut(s) 499
BstV2I GAAGAC 1 cut(s) 625
BsuRI GGCC 5 cut(s) 182, 213, 796, 873, 887
BtgZI GCGATG 2 cut(s) 85, 785
BtsCI GGATG 1 cut(s) 623
BtsI GCAGTG 2 cut(s) 18, 259
BtsIMutI CAGTG 2 cut(s) 18, 259
Cac8I GCNNGC 2 cut(s) 250, 747
CfoI GCGC 4 cut(s) 382, 745, 772, 914
Cfr13I GGNCC 2 cut(s) 794, 886
CseI GACGC 2 cut(s) 613, 857
Csp6I GTAC 2 cut(s) 340, 586
CviAII CATG 5 cut(s) 21, 115, 146, 564, 1014
CviQI GTAC 2 cut(s) 340, 586
DdeI CTNAG 5 cut(s) 43, 86, 300, 540, 697
DinI GGCGCC 1 cut(s) 913
DpnI GATC 5 cut(s) 113, 240, 314, 371, 979
DpnII GATC 5 cut(s) 111, 238, 312, 369, 977
EaeI YGGCCR 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 941
EarI CTCTTC 1 cut(s) 941
EciI GGCGGA 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 67, 659
Eco24I GRGCYC 2 cut(s) 299, 332
Eco57I CTGAAG 1 cut(s) 455
EcoNI CCTNNNNNAGG 1 cut(s) 663
EcoO109I RGGNCCY 1 cut(s) 886
EcoRI GAATTC 1 cut(s) 304
EcoRII CCWGG 3 cut(s) 395, 436, 882
EcoT14I CCWWGG 2 cut(s) 67, 659
EcoT38I GRGCYC 2 cut(s) 299, 332
EgeI GGCGCC 1 cut(s) 913
EheI GGCGCC 1 cut(s) 913
ErhI CCWWGG 2 cut(s) 67, 659
Esp3I CGTCTC 1 cut(s) 596
FaeI CATG 5 cut(s) 24, 118, 149, 567, 1017
FaqI GGGAC 2 cut(s) 790, 815
FatI CATG 5 cut(s) 20, 114, 145, 563, 1013
FbaI TGATCA 1 cut(s) 369
FblI GTMKAC 1 cut(s) 942
Fnu4HI GCNGC 4 cut(s) 279, 488, 797, 1003
FokI GGATG 1 cut(s) 610
FriOI GRGCYC 2 cut(s) 299, 332
Fsp4HI GCNGC 4 cut(s) 279, 488, 797, 1003
FspBI CTAG 3 cut(s) 581, 660, 665
GlaI GCGC 4 cut(s) 381, 744, 771, 913
GluI GCNGC 4 cut(s) 279, 488, 797, 1003
GsaI CCCAGC 1 cut(s) 642
HaeII RGCGCY 1 cut(s) 915
HaeIII GGCC 5 cut(s) 182, 213, 796, 873, 887
HapII CCGG 7 cut(s) 105, 179, 383, 464, 870, 921, 968
HgaI GACGC 2 cut(s) 613, 857
HhaI GCGC 4 cut(s) 382, 745, 772, 914
Hin1I GRCGYC 2 cut(s) 849, 912
Hin1II CATG 5 cut(s) 24, 118, 149, 567, 1017
Hin6I GCGC 4 cut(s) 380, 743, 770, 912
HinP1I GCGC 4 cut(s) 380, 743, 770, 912
HincII GTYRAC 1 cut(s) 170
HindII GTYRAC 1 cut(s) 170
HindIII AAGCTT 1 cut(s) 524
HinfI GANTC 8 cut(s) 81, 152, 425, 570, 896, 924, 956, 964
HpaII CCGG 7 cut(s) 105, 179, 383, 464, 870, 921, 968
HphI GGTGA 1 cut(s) 552
Hpy166II GTNNAC 2 cut(s) 170, 943
Hpy188I TCNGA 6 cut(s) 89, 312, 430, 474, 541, 895
Hpy188III TCNNGA 5 cut(s) 128, 416, 724, 921, 981
Hpy8I GTNNAC 2 cut(s) 170, 943
Hpy99I CGWCG 5 cut(s) 197, 200, 295, 851, 964
HpyAV CCTTC 3 cut(s) 166, 343, 940
HpyCH4III ACNGT 1 cut(s) 647
HpyCH4V TGCA 4 cut(s) 11, 149, 567, 1002
HpyF10VI GCNNNNNNNGC 2 cut(s) 249, 648
HpyF3I CTNAG 5 cut(s) 43, 86, 300, 540, 697
Hsp92I GRCGYC 2 cut(s) 849, 912
Hsp92II CATG 5 cut(s) 24, 118, 149, 567, 1017
HspAI GCGC 4 cut(s) 380, 743, 770, 912
KasI GGCGCC 1 cut(s) 911
Kpn2I TCCGGA 1 cut(s) 920
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 5 cut(s) 111, 238, 312, 369, 977
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 1 cut(s) 499
LweI GCATC 1 cut(s) 808
MaeI CTAG 3 cut(s) 581, 660, 665
MalI GATC 5 cut(s) 113, 240, 314, 371, 979
MboI GATC 5 cut(s) 111, 238, 312, 369, 977
MboII GAAGA 4 cut(s) 151, 221, 625, 928
MhlI GDGCHC 3 cut(s) 261, 299, 332
MluCI AATT 3 cut(s) 304, 317, 549
Mly113I GGCGCC 1 cut(s) 912
MlyI GAGTC 5 cut(s) 75, 146, 434, 918, 965
MroI TCCGGA 1 cut(s) 920
MseI TTAA 3 cut(s) 51, 284, 729
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 7 cut(s) 105, 179, 383, 464, 870, 921, 968
MspR9I CCNGG 3 cut(s) 397, 438, 884
Mva1269I GAATGC 1 cut(s) 701
MvaI CCWGG 3 cut(s) 397, 438, 884
MvnI CGCG 3 cut(s) 278, 743, 772
MwoI GCNNNNNNNGC 2 cut(s) 249, 648
NarI GGCGCC 1 cut(s) 912
NdeII GATC 5 cut(s) 111, 238, 312, 369, 977
NlaIII CATG 5 cut(s) 24, 118, 149, 567, 1017
NlaIV GGNNCC 3 cut(s) 73, 352, 913
NmeAIII GCCGAG 3 cut(s) 189, 888, 1016
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 951
PctI GAATGC 1 cut(s) 701
PfeI GAWTC 3 cut(s) 570, 896, 964
PkrI GCNGC 4 cut(s) 280, 489, 798, 1004
Ple19I CGATCG 1 cut(s) 241
PleI GAGTC 5 cut(s) 75, 146, 433, 918, 964
PluTI GGCGCC 1 cut(s) 915
PpsI GAGTC 5 cut(s) 75, 146, 433, 918, 964
PshAI GACNNNNGTC 1 cut(s) 608
Psp6I CCWGG 3 cut(s) 395, 436, 882
PspFI CCCAGC 1 cut(s) 638
PspGI CCWGG 3 cut(s) 395, 436, 882
PspN4I GGNNCC 3 cut(s) 73, 352, 913
PspPI GGNCC 2 cut(s) 794, 886
PvuI CGATCG 1 cut(s) 241
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 2 cut(s) 341, 587
RsaNI GTAC 2 cut(s) 340, 586
SaqAI TTAA 3 cut(s) 51, 284, 729
SatI GCNGC 4 cut(s) 279, 488, 797, 1003
Sau3AI GATC 5 cut(s) 111, 238, 312, 369, 977
Sau96I GGNCC 2 cut(s) 794, 886
ScaI AGTACT 1 cut(s) 587
SchI GAGTC 5 cut(s) 75, 146, 434, 918, 965
ScrFI CCNGG 3 cut(s) 397, 438, 884
SduI GDGCHC 3 cut(s) 261, 299, 332
SfaNI GCATC 1 cut(s) 808
SfcI CTRYAG 1 cut(s) 454
SfoI GGCGCC 1 cut(s) 913
Sse9I AATT 3 cut(s) 304, 317, 549
SsiI CCGC 4 cut(s) 264, 278, 755, 797
SspDI GGCGCC 1 cut(s) 911
SspMI CTAG 3 cut(s) 581, 660, 665
StyD4I CCNGG 3 cut(s) 395, 436, 882
StyI CCWWGG 2 cut(s) 67, 659
TaaI ACNGT 1 cut(s) 647
TaqI TCGA 9 cut(s) 120, 192, 237, 293, 336, 814, 846, 962, 980
TaqII GACCGA 2 cut(s) 155, 776
TasI AATT 3 cut(s) 304, 317, 549
TatI WGTACW 2 cut(s) 339, 585
TauI GCSGC 2 cut(s) 281, 799
TfiI GAWTC 3 cut(s) 570, 896, 964
Tru1I TTAA 3 cut(s) 51, 284, 729
Tru9I TTAA 3 cut(s) 51, 284, 729
TscAI CASTG 2 cut(s) 18, 259
TseI GCWGC 2 cut(s) 487, 1002
TspDTI ATGAA 3 cut(s) 151, 379, 562
TspGWI ACGGA 1 cut(s) 868
TspRI CASTG 2 cut(s) 18, 259
XagI CCTNNNNNAGG 1 cut(s) 663
XapI RAATTY 2 cut(s) 304, 317
XmaJI CCTAGG 1 cut(s) 659
XmiI GTMKAC 1 cut(s) 942
XspI CTAG 3 cut(s) 581, 660, 665
ZrmI AGTACT 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.