Rmu_ssc0000482.1_g000018

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000482.1
OG0019236
Physical Location & Seq
Reverse (-)
67507 .. 69401
1895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000482.1_g000018.1.cds

Sequence Viewer

Length: 735 bp
atggagtcccacttgggaattggttggcactttctcaagttgggggataagggtagaggcgtcatgggcactattagaaatccagagaagaggaagagaagaagagcttcgagcacttcgtcatctgcgggatcggatcggatcaccagagggttccacagcttcgctgaaatgcacttgccatctttgcttgaccagttccctcccgccaaccacactctataccctcctccgccgcctcagctccccactgtaagatttgattccagtgatgtgacgaggtttccatggagctatccgattgttctaactgtgaggtgccgggggtggagaatgggtcatcgatgtcgcaggcggcgcgtgaggtggagacgaacttttccagcctctgaatcttggccgtcacactcgctcctcctccgattggcttcatatgctgctgattataaaaccccactctgcatcaaaatcaatttcttgttcttctttctttcttcccctaaagagcactattgtgaaacaaagatgaaatctttgagtagtgtaggacttggtttgagtgtggtttttggttgcttattcttggctccaattgttgagctttacttcttgctgtggtggaagaagaggctgaccaatagagagatagaagatgactacagcagcacatcaaaggaggtgttccacatgttctgctggagaaggacttctaagtctttgagccacatggcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

28.45

Weight (kDa)

10.34

Isoelectric Point (pI)

66.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019236)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12390 FvH4_5g12410
malus_domestica MD06G1214100.v1.1
rosa_multiflora Rmu_ssc0000482.1_g000018
rosa_roxburghii Rroxscaffold_3G00232330
rosa_rugosa Rorug06G0033100 Rorug07G0110400
rosa_samantha Rh5DG016700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 447
AasI GACNNNNNNGTC 1 cut(s) 712
AccB1I GGYRCC 1 cut(s) 318
AccII CGCG 1 cut(s) 360
AciI CCGC 5 cut(s) 128, 207, 233, 236, 355
AclWI GGATC 3 cut(s) 139, 144, 149
AcoI YGGCCR 1 cut(s) 398
AcyI GRCGYC 1 cut(s) 60
AfiI CCNNNNNNNGG 1 cut(s) 424
AflIII ACRYGT 1 cut(s) 687
AleI CACNNNNGTG 1 cut(s) 513
AluBI AGCT 5 cut(s) 107, 162, 244, 294, 601
AluI AGCT 5 cut(s) 107, 162, 244, 294, 601
Alw21I GWGCWC 2 cut(s) 116, 510
Alw26I GTCTC 1 cut(s) 364
AlwI GGATC 3 cut(s) 139, 144, 149
AlwNI CAGNNNCTG 1 cut(s) 389
AoxI GGCC 1 cut(s) 398
ApeKI GCWGC 2 cut(s) 437, 663
Asp700I GAANNNNTTC 2 cut(s) 106, 706
AspLEI GCGC 1 cut(s) 360
AsuC2I CCSGG 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 71
BanI GGYRCC 1 cut(s) 318
Bbv12I GWGCWC 2 cut(s) 116, 510
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 424, 675
BccI CCATC 1 cut(s) 190
BceAI ACGGC 1 cut(s) 385
BcnI CCSGG 1 cut(s) 323
BcoDI GTCTC 1 cut(s) 364
BfmI CTRYAG 1 cut(s) 658
BisI GCNGC 4 cut(s) 236, 356, 438, 664
BlsI GCNGC 4 cut(s) 237, 357, 439, 665
Bme1390I CCNGG 1 cut(s) 323
BmiI GGNNCC 3 cut(s) 155, 320, 588
BmrFI CCNGG 1 cut(s) 323
BmsI GCATC 1 cut(s) 471
BpmI CTGGAG 1 cut(s) 718
Bpu10I CCTNAGC 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 20
BpuMI CCSGG 1 cut(s) 323
Bsa29I ATCGAT 1 cut(s) 343
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 2 cut(s) 287, 322
BsaXI ACNNNNNCTCC 4 cut(s) 322, 352, 396, 426
Bsc4I CCNNNNNNNGG 1 cut(s) 424
Bse1I ACTGG 2 cut(s) 196, 267
BseCI ATCGAT 1 cut(s) 343
BseDI CCNNGG 2 cut(s) 287, 322
BseLI CCNNNNNNNGG 1 cut(s) 424
BseMII CTCAG 1 cut(s) 254
BseNI ACTGG 2 cut(s) 196, 267
BseRI GAGGAG 3 cut(s) 219, 404, 407
BseSI GKGCMC 1 cut(s) 71
BseXI GCAGC 2 cut(s) 424, 675
Bsh1236I CGCG 1 cut(s) 360
BshFI GGCC 1 cut(s) 400
BshNI GGYRCC 1 cut(s) 318
BshVI ATCGAT 1 cut(s) 343
BsiHKAI GWGCWC 2 cut(s) 116, 510
BsiSI CCGG 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 424
BsmAI GTCTC 1 cut(s) 364
BsmBI CGTCTC 1 cut(s) 364
BsnI GGCC 1 cut(s) 400
Bsp1286I GDGCHC 3 cut(s) 71, 116, 510
Bsp143I GATC 3 cut(s) 131, 136, 141
Bsp19I CCATGG 1 cut(s) 287
BspACI CCGC 5 cut(s) 128, 207, 233, 236, 355
BspANI GGCC 1 cut(s) 400
BspCNI CTCAG 1 cut(s) 253
BspDI ATCGAT 1 cut(s) 343
BspFNI CGCG 1 cut(s) 360
BspLI GGNNCC 3 cut(s) 155, 320, 588
BspPI GGATC 3 cut(s) 139, 144, 149
BspQI GCTCTTC 1 cut(s) 97
BspT107I GGYRCC 1 cut(s) 318
BsrI ACTGG 2 cut(s) 196, 267
BssECI CCNNGG 2 cut(s) 287, 322
BssMI GATC 3 cut(s) 131, 136, 141
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 287
Bst4CI ACNGT 2 cut(s) 253, 313
Bst6I CTCTTC 4 cut(s) 83, 89, 97, 620
BstACI GRCGYC 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 353
BstDEI CTNAG 2 cut(s) 240, 711
BstDSI CCRYGG 1 cut(s) 287
BstFNI CGCG 1 cut(s) 360
BstHHI GCGC 1 cut(s) 360
BstKTI GATC 3 cut(s) 134, 139, 144
BstMAI GTCTC 1 cut(s) 364
BstMBI GATC 3 cut(s) 131, 136, 141
BstMWI GCNNNNNNNGC 5 cut(s) 66, 187, 241, 357, 434
BstNSI RCATGY 1 cut(s) 691
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 1 cut(s) 658
BstSLI GKGCMC 1 cut(s) 71
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 424, 675
Bsu15I ATCGAT 1 cut(s) 343
BsuRI GGCC 1 cut(s) 400
BsuTUI ATCGAT 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 287
BtsIMutI CAGTG 2 cut(s) 249, 274
Cac8I GCNNGC 1 cut(s) 353
CaiI CAGNNNCTG 1 cut(s) 389
CfoI GCGC 1 cut(s) 360
ClaI ATCGAT 1 cut(s) 343
CseI GACGC 1 cut(s) 49
CviAII CATG 4 cut(s) 64, 288, 688, 727
DdeI CTNAG 2 cut(s) 240, 711
DpnI GATC 3 cut(s) 133, 138, 143
DpnII GATC 3 cut(s) 131, 136, 141
DrdI GACNNNNNNGTC 1 cut(s) 712
DseDI GACNNNNNNGTC 1 cut(s) 712
EaeI YGGCCR 1 cut(s) 398
Eam1104I CTCTTC 4 cut(s) 83, 89, 97, 620
EarI CTCTTC 4 cut(s) 83, 89, 97, 620
EciI GGCGGA 1 cut(s) 222
Eco130I CCWWGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 287
ErhI CCWWGG 1 cut(s) 287
Esp3I CGTCTC 1 cut(s) 364
FaeI CATG 4 cut(s) 67, 291, 691, 730
FaiI YATR 8 cut(s) 65, 223, 289, 433, 435, 447, 689, 728
FalI AAGNNNNNCTT 2 cut(s) 91, 123
FatI CATG 4 cut(s) 63, 287, 687, 726
FauI CCCGC 2 cut(s) 121, 214
FauNDI CATATG 1 cut(s) 433
Fnu4HI GCNGC 4 cut(s) 236, 356, 438, 664
Fsp4HI GCNGC 4 cut(s) 236, 356, 438, 664
GlaI GCGC 1 cut(s) 359
GluI GCNGC 4 cut(s) 236, 356, 438, 664
GsuI CTGGAG 1 cut(s) 718
HaeIII GGCC 1 cut(s) 400
HapII CCGG 1 cut(s) 322
HgaI GACGC 1 cut(s) 49
HhaI GCGC 1 cut(s) 360
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 4 cut(s) 67, 291, 691, 730
Hin6I GCGC 1 cut(s) 358
HinP1I GCGC 1 cut(s) 358
HinfI GANTC 3 cut(s) 5, 263, 392
HpaII CCGG 1 cut(s) 322
HphI GGTGA 1 cut(s) 136
Hpy188I TCNGA 5 cut(s) 136, 141, 300, 391, 422
Hpy188III TCNNGA 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 696
HpyCH4III ACNGT 2 cut(s) 253, 313
HpyCH4V TGCA 2 cut(s) 175, 462
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 187, 241, 357, 434
HpyF3I CTNAG 2 cut(s) 240, 711
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 4 cut(s) 67, 291, 691, 730
HspAI GCGC 1 cut(s) 358
Kzo9I GATC 3 cut(s) 131, 136, 141
LguI GCTCTTC 1 cut(s) 97
LmnI GCTCC 4 cut(s) 249, 291, 417, 592
LpnPI CCDG 8 cut(s) 96, 160, 209, 280, 335, 337, 396, 682
Lsp1109I GCAGC 2 cut(s) 424, 675
LweI GCATC 1 cut(s) 471
MaeIII GTNAC 2 cut(s) 274, 402
MalI GATC 3 cut(s) 133, 138, 143
MboI GATC 3 cut(s) 131, 136, 141
MboII GAAGA 9 cut(s) 100, 106, 111, 114, 475, 486, 634, 637, 662
MfeI CAATTG 1 cut(s) 591
MhlI GDGCHC 3 cut(s) 71, 116, 510
MluCI AATT 3 cut(s) 18, 472, 591
MlyI GAGTC 1 cut(s) 14
MroXI GAANNNNTTC 2 cut(s) 106, 706
MseI TTAA 1 cut(s) 733
MslI CAYNNNNRTG 1 cut(s) 513
MspI CCGG 1 cut(s) 322
MspR9I CCNGG 1 cut(s) 323
MunI CAATTG 1 cut(s) 591
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 5 cut(s) 66, 187, 241, 357, 434
NciI CCSGG 1 cut(s) 323
NcoI CCATGG 1 cut(s) 287
NdeI CATATG 1 cut(s) 433
NdeII GATC 3 cut(s) 131, 136, 141
NlaIII CATG 4 cut(s) 67, 291, 691, 730
NlaIV GGNNCC 3 cut(s) 155, 320, 588
NmuCI GTSAC 2 cut(s) 274, 402
NspI RCATGY 1 cut(s) 691
OliI CACNNNNGTG 1 cut(s) 513
PciI ACATGT 1 cut(s) 687
PciSI GCTCTTC 1 cut(s) 97
PcsI WCGNNNNNNNCGW 1 cut(s) 116
PdmI GAANNNNTTC 2 cut(s) 106, 706
PfeI GAWTC 2 cut(s) 263, 392
PkrI GCNGC 4 cut(s) 237, 357, 439, 665
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PscI ACATGT 1 cut(s) 687
PsiI TTATAA 1 cut(s) 447
PspN4I GGNNCC 3 cut(s) 155, 320, 588
PstNI CAGNNNCTG 1 cut(s) 389
RseI CAYNNNNRTG 1 cut(s) 513
SapI GCTCTTC 1 cut(s) 97
SaqAI TTAA 1 cut(s) 733
SatI GCNGC 4 cut(s) 236, 356, 438, 664
Sau3AI GATC 3 cut(s) 131, 136, 141
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 3 cut(s) 71, 116, 510
SetI ASST 9 cut(s) 109, 164, 246, 284, 296, 320, 368, 603, 681
SfaNI GCATC 1 cut(s) 471
SfcI CTRYAG 1 cut(s) 658
SmiMI CAYNNNNRTG 1 cut(s) 513
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
Sse9I AATT 3 cut(s) 18, 472, 591
SsiI CCGC 5 cut(s) 128, 207, 233, 236, 355
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 2 cut(s) 253, 313
TaqI TCGA 2 cut(s) 110, 343
TasI AATT 3 cut(s) 18, 472, 591
TauI GCSGC 2 cut(s) 238, 358
TfiI GAWTC 2 cut(s) 263, 392
Tru1I TTAA 1 cut(s) 733
Tru9I TTAA 1 cut(s) 733
TscAI CASTG 2 cut(s) 256, 274
TseFI GTSAC 2 cut(s) 274, 402
TseI GCWGC 2 cut(s) 437, 663
Tsp45I GTSAC 2 cut(s) 274, 402
TspDTI ATGAA 2 cut(s) 420, 542
TspRI CASTG 2 cut(s) 256, 274
XceI RCATGY 1 cut(s) 691
XcmI CCANNNNNNNNNTGG 1 cut(s) 17
XmnI GAANNNNTTC 2 cut(s) 106, 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.