Rroxscaffold_1G00000900

nucleic acid binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0018194
Physical Location & Seq
Forward (+)
1312671 .. 1323153
10483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00000900.1

Sequence Viewer

Length: 1335 bp
ATGCGGACACTGTTTGTAACTCAGCTGCATGCAAGTGTGAGCGAGAAGAGGCTCAAATCGGTATTTGAGAAATATGGTCCCGTTAAAAGGACGAAAATTATTCCTTACCCGTGGAAGAAGAATTGTGCTTTTGTGGAGTTTGAGCGGGAGAGTGATAGTCAAGATGCGTTGGTGGATATGCGTTTCAAATTCTTCCCGGACAAATGGCAATGGAAAGTGTGCTATGCTCACTCGGATGATCCTGGAAAGAGTGTTGTAGAAGAGAATGTTGAAGAAGTTGATTGGCTACTAGTGCCTCAATCATCATCCGACTCAAGAAAGGCTCTTGAAGAAGATCATTCCTCATCGTACTCTGGGTTTGTATCAGACCGCGGCGGAAGAATGCGGACACTGTTTGTAACTCAGCTGCATGCAAGTGTGAGCGAGAAGAGGCTCAAATCGGTATTTGAAAAATATGGTCCCGTTAAAAGGACGAAAATTATTCCTTACCTGTGGAAGATGAATTGTGCATTTGTGGAGTTTGAGCGGGAGAGTGATAGTGAAGATGCATTGGTGGATATGCGTTTCAAAAGCTTCCTAGGCAAATGGCAGTGGAAAGTGCAATATGCTCATTCGGATGATGCTGGAAAGGGTGTTGAAGAAGACTGTGTTGAAGAAGTTTATTGGCTACTTGTGCCTCGATCGTCATCCGACTCAAGAAAGGCTCTTGAAGAAGATCATTCCTCATCCCACTCTGGTACTTCACTATTTTTCATTGCTACAAATGGGTCGCACCATCGCAAAGCTCTCTTCCTAGGTTTAAGTTGCCTCCTCTCCCACTTGCCTCGACCACTGCTGACATTAAGTACCTCCGGATATACTGTGAAAGCTTTCTGGAGGAAGAACACTTCTGGTTCTGTTCTGATCTGGCGAGAAGCAAAATATAATCTTTGCATCCAGAGAACAAAAGGTAACTATATAATTGCACAAGAAGAAGTGACAGATTCAAAGTATCGCAAATTTCGTGACGTTGGAATTAGTCCTGAAATGATGGCTGTTTATGATAACATGTTTAGGGGTAGCACAGCCCTAGGTCACTCTGTCATGATTCCCTCAGCTACTATAGATATTGAAGAGGTGGTGGAGGAATCTGAGCATAATGACATTTACGGAGATGATGAGGAGATGGATCAACAAGGTGAACCAAGAGGGAAAAAGAGAAAAACTGTGGAGTGCCAAACTGGGCGTAATAAAGAAAAAAAGATAAAGGAGTTATGGGAGGGCCAAAGGGGAAGAAAGAAAAGGTGGGAGGTGCAGCTCAATTGTCTAAACAAATTGATCGTCTTGTTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0000375 GO:0000377 GO:0000381 GO:0000398 GO:0001817 GO:0001818 GO:0002119 GO:0002164 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005685 GO:0005737 GO:0005764 GO:0005773 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006397 GO:0006417 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006952 GO:0006970 GO:0006972 GO:0006979 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007276 GO:0007281 GO:0007568 GO:0008104 GO:0008143 GO:0008150 GO:0008152 GO:0008219 GO:0008284 GO:0008340 GO:0008380 GO:0008543 GO:0009266 GO:0009314 GO:0009408 GO:0009411 GO:0009416 GO:0009628 GO:0009719 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010259 GO:0010467 GO:0010468 GO:0010494 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0016070 GO:0016071 GO:0017091 GO:0017145 GO:0017148 GO:0019219 GO:0019222 GO:0019953 GO:0022412 GO:0022414 GO:0023052 GO:0030154 GO:0030532 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033036 GO:0033365 GO:0034248 GO:0034249 GO:0034613 GO:0034641 GO:0035770 GO:0036464 GO:0040012 GO:0042035 GO:0042036 GO:0042127 GO:0042221 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043484 GO:0044237 GO:0044238 GO:0044344 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0046483 GO:0048024 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048609 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051179 GO:0051239 GO:0051241 GO:0051246 GO:0051248 GO:0051252 GO:0051301 GO:0051641 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070717 GO:0070727 GO:0070848 GO:0070887 GO:0071310 GO:0071363 GO:0071495 GO:0071704 GO:0071774 GO:0080090 GO:0090304 GO:0097159 GO:0097165 GO:0097525 GO:0120114 GO:1901360 GO:1901363 GO:1903311 GO:1903506 GO:1903608 GO:1904035 GO:1904037 GO:1990904 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

444

Amino Acids

51.43

Weight (kDa)

8.42

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 4 - 67 6.5e-11 RNA recognition motif
RRM_1 PF00076 131 - 195 1.1e-10 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018194)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0083151
rosa_laevigata RLG00000037044
rosa_multiflora Rmu_sc0001287.1_g000002 Rmu_sc0002184.1_g000018
rosa_roxburghii Rroxscaffold_1G00000900
rosa_rugosa Rorug05G0440000
rosa_samantha Rh5AG532300 Rh5BG563600 Rh5CG585700 Rh5DG571700
rosa_wichuraiana Rw5G050070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 145, 526
AccII CGCG 1 cut(s) 372
AccIII TCCGGA 1 cut(s) 851
AciI CCGC 7 cut(s) 4, 145, 370, 372, 375, 385, 526
AclWI GGATC 2 cut(s) 233, 1176
AcsI RAATTY 2 cut(s) 188, 998
AfaI GTAC 3 cut(s) 350, 739, 847
AfiI CCNNNNNNNGG 2 cut(s) 87, 468
AflIII ACRYGT 1 cut(s) 1047
AhlI ACTAGT 1 cut(s) 289
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 7 cut(s) 25, 406, 573, 785, 869, 1097, 1297
AluI AGCT 7 cut(s) 25, 406, 573, 785, 869, 1097, 1297
AlwI GGATC 2 cut(s) 233, 1176
Aor13HI TCCGGA 1 cut(s) 851
AoxI GGCC 1 cut(s) 1261
ApeKI GCWGC 3 cut(s) 25, 406, 1294
ApoI RAATTY 2 cut(s) 188, 998
ArsI GACNNNNNNTTYG 1 cut(s) 1305
Asp700I GAANNNNTTC 1 cut(s) 869
AspA2I CCTAGG 3 cut(s) 577, 793, 1069
AspS9I GGNCC 3 cut(s) 77, 458, 1261
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 1190
AvaII GGWCC 2 cut(s) 77, 458
AvrII CCTAGG 3 cut(s) 577, 793, 1069
BbsI GAAGAC 1 cut(s) 648
BbvCI CCTCAGC 1 cut(s) 1093
BbvI GCAGC 3 cut(s) 12, 393, 1306
BccI CCATC 3 cut(s) 783, 1024, 1159
BciT130I CCWGG 1 cut(s) 243
BcnI CCSGG 1 cut(s) 197
BcuI ACTAGT 1 cut(s) 289
BfaI CTAG 4 cut(s) 290, 578, 794, 1070
BfmI CTRYAG 1 cut(s) 1101
BisI GCNGC 4 cut(s) 26, 373, 407, 1295
BlnI CCTAGG 3 cut(s) 577, 793, 1069
BlsI GCNGC 4 cut(s) 27, 374, 408, 1296
Bme1390I CCNGG 2 cut(s) 197, 243
Bme18I GGWCC 2 cut(s) 77, 458
BmgT120I GGNCC 3 cut(s) 77, 458, 1261
BmiI GGNNCC 2 cut(s) 79, 460
BmrFI CCNGG 2 cut(s) 197, 243
BmrI ACTGGG 1 cut(s) 1230
BmsI GCATC 4 cut(s) 154, 535, 610, 942
BmuI ACTGGG 1 cut(s) 1230
BpiI GAAGAC 1 cut(s) 648
BpmI CTGGAG 1 cut(s) 895
Bpu10I CCTNAGC 1 cut(s) 1093
BpuEI CTTGAG 2 cut(s) 298, 679
BpuMI CCSGG 1 cut(s) 197
BsaBI GATNNNNATC 1 cut(s) 685
BsaJI CCNNGG 5 cut(s) 110, 370, 577, 793, 1069
BsaWI WCCGGW 1 cut(s) 851
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 468
Bse1I ACTGG 1 cut(s) 1225
Bse3DI GCAATG 2 cut(s) 215, 753
Bse8I GATNNNNATC 1 cut(s) 685
BseAI TCCGGA 1 cut(s) 851
BseBI CCWGG 1 cut(s) 243
BseDI CCNNGG 5 cut(s) 110, 370, 577, 793, 1069
BseGI GGATG 6 cut(s) 241, 305, 622, 686, 725, 933
BseJI GATNNNNATC 1 cut(s) 685
BseLI CCNNNNNNNGG 2 cut(s) 87, 468
BseMI GCAATG 2 cut(s) 215, 753
BseMII CTCAG 4 cut(s) 35, 416, 1107, 1122
BseNI ACTGG 1 cut(s) 1225
BseRI GAGGAG 2 cut(s) 800, 1175
BseXI GCAGC 3 cut(s) 12, 393, 1306
BsgI GTGCAG 1 cut(s) 1313
Bsh1236I CGCG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 683
BshFI GGCC 1 cut(s) 1263
BsiEI CGRYCG 1 cut(s) 683
BsiSI CCGG 2 cut(s) 197, 852
BslFI GGGAC 2 cut(s) 63, 444
BslI CCNNNNNNNGG 2 cut(s) 87, 468
BsmFI GGGAC 2 cut(s) 63, 444
BsmI GAATGC 1 cut(s) 387
BsnI GGCC 1 cut(s) 1263
Bsp13I TCCGGA 1 cut(s) 851
Bsp143I GATC 7 cut(s) 238, 334, 680, 715, 903, 1168, 1317
BspACI CCGC 7 cut(s) 4, 145, 370, 372, 375, 385, 526
BspANI GGCC 1 cut(s) 1263
BspCNI CTCAG 4 cut(s) 34, 415, 1106, 1123
BspEI TCCGGA 1 cut(s) 851
BspFNI CGCG 1 cut(s) 372
BspHI TCATGA 1 cut(s) 1083
BspLI GGNNCC 2 cut(s) 79, 460
BspPI GGATC 2 cut(s) 233, 1176
BsrBI CCGCTC 2 cut(s) 145, 526
BsrDI GCAATG 2 cut(s) 215, 753
BsrI ACTGG 1 cut(s) 1225
BssECI CCNNGG 5 cut(s) 110, 370, 577, 793, 1069
BssMI GATC 7 cut(s) 238, 334, 680, 715, 903, 1168, 1317
BssT1I CCWWGG 3 cut(s) 577, 793, 1069
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 5 cut(s) 12, 393, 647, 862, 1207
Bst6I CTCTTC 5 cut(s) 41, 255, 422, 794, 1107
BstC8I GCNNGC 2 cut(s) 30, 411
BstDEI CTNAG 4 cut(s) 21, 402, 1093, 1131
BstDSI CCRYGG 2 cut(s) 110, 370
BstF5I GGATG 6 cut(s) 241, 305, 622, 686, 725, 933
BstFNI CGCG 1 cut(s) 372
BstKTI GATC 7 cut(s) 241, 337, 683, 718, 906, 1171, 1320
BstMBI GATC 7 cut(s) 238, 334, 680, 715, 903, 1168, 1317
BstMCI CGRYCG 1 cut(s) 683
BstMWI GCNNNNNNNGC 3 cut(s) 292, 579, 673
BstNI CCWGG 1 cut(s) 243
BstNSI RCATGY 3 cut(s) 32, 413, 1051
BstSCI CCNGG 2 cut(s) 195, 241
BstSFI CTRYAG 1 cut(s) 1101
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 3 cut(s) 12, 393, 1306
BstV2I GAAGAC 1 cut(s) 648
BsuRI GGCC 1 cut(s) 1263
BtgI CCRYGG 2 cut(s) 110, 370
BtgZI GCGATG 1 cut(s) 761
BtsCI GGATG 6 cut(s) 241, 305, 622, 686, 725, 933
BtsI GCAGTG 2 cut(s) 596, 830
BtsIMutI CAGTG 4 cut(s) 8, 389, 596, 830
Cac8I GCNNGC 2 cut(s) 30, 411
CciI TCATGA 1 cut(s) 1083
Cfr13I GGNCC 3 cut(s) 77, 458, 1261
Cfr42I CCGCGG 1 cut(s) 373
Csp6I GTAC 3 cut(s) 349, 738, 846
CviAII CATG 4 cut(s) 29, 410, 1048, 1084
CviQI GTAC 3 cut(s) 349, 738, 846
DdeI CTNAG 4 cut(s) 21, 402, 1093, 1131
DpnI GATC 7 cut(s) 240, 336, 682, 717, 905, 1170, 1319
DpnII GATC 7 cut(s) 238, 334, 680, 715, 903, 1168, 1317
Eam1104I CTCTTC 5 cut(s) 41, 255, 422, 794, 1107
EarI CTCTTC 5 cut(s) 41, 255, 422, 794, 1107
EciI GGCGGA 1 cut(s) 390
Eco130I CCWWGG 3 cut(s) 577, 793, 1069
Eco47I GGWCC 2 cut(s) 77, 458
EcoRII CCWGG 1 cut(s) 241
EcoT14I CCWWGG 3 cut(s) 577, 793, 1069
EcoT22I ATGCAT 1 cut(s) 550
ErhI CCWWGG 3 cut(s) 577, 793, 1069
FaeI CATG 4 cut(s) 32, 413, 1051, 1087
FaqI GGGAC 2 cut(s) 63, 444
FatI CATG 4 cut(s) 28, 409, 1047, 1083
FauI CCCGC 2 cut(s) 138, 519
Fnu4HI GCNGC 4 cut(s) 26, 373, 407, 1295
FokI GGATG 6 cut(s) 248, 292, 629, 673, 712, 920
Fsp4HI GCNGC 4 cut(s) 26, 373, 407, 1295
FspBI CTAG 4 cut(s) 290, 578, 794, 1070
GluI GCNGC 4 cut(s) 26, 373, 407, 1295
GsuI CTGGAG 1 cut(s) 895
HaeIII GGCC 1 cut(s) 1263
HapII CCGG 2 cut(s) 197, 852
Hin1II CATG 4 cut(s) 32, 413, 1051, 1087
HindIII AAGCTT 2 cut(s) 571, 867
HinfI GANTC 5 cut(s) 311, 692, 983, 1087, 1127
HpaII CCGG 2 cut(s) 197, 852
HphI GGTGA 1 cut(s) 1190
Hpy166II GTNNAC 1 cut(s) 1181
Hpy188I TCNGA 7 cut(s) 235, 310, 367, 616, 691, 903, 1132
Hpy8I GTNNAC 1 cut(s) 1181
HpyCH4III ACNGT 5 cut(s) 12, 393, 647, 862, 1207
HpyCH4IV ACGT 1 cut(s) 1008
HpyF10VI GCNNNNNNNGC 3 cut(s) 292, 579, 673
HpyF3I CTNAG 4 cut(s) 21, 402, 1093, 1131
HpySE526I ACGT 1 cut(s) 1008
Hsp92II CATG 4 cut(s) 32, 413, 1051, 1087
Kpn2I TCCGGA 1 cut(s) 851
KspI CCGCGG 1 cut(s) 373
Kzo9I GATC 7 cut(s) 238, 334, 680, 715, 903, 1168, 1317
Lsp1109I GCAGC 3 cut(s) 12, 393, 1306
LweI GCATC 4 cut(s) 154, 535, 610, 942
MaeI CTAG 4 cut(s) 290, 578, 794, 1070
MaeII ACGT 1 cut(s) 1008
MaeIII GTNAC 6 cut(s) 16, 397, 950, 976, 1004, 1073
MalI GATC 7 cut(s) 240, 336, 682, 717, 905, 1170, 1319
MbiI CCGCTC 2 cut(s) 145, 526
MboI GATC 7 cut(s) 238, 334, 680, 715, 903, 1168, 1317
MfeI CAATTG 1 cut(s) 1300
MlyI GAGTC 2 cut(s) 305, 686
MmeI TCCRAC 3 cut(s) 333, 714, 991
Mph1103I ATGCAT 1 cut(s) 550
MroI TCCGGA 1 cut(s) 851
MroXI GAANNNNTTC 1 cut(s) 869
MseI TTAA 4 cut(s) 84, 465, 800, 842
MslI CAYNNNNRTG 4 cut(s) 33, 234, 414, 615
MspA1I CMGCKG 3 cut(s) 25, 372, 406
MspI CCGG 2 cut(s) 197, 852
MspR9I CCNGG 2 cut(s) 197, 243
MunI CAATTG 1 cut(s) 1300
Mva1269I GAATGC 1 cut(s) 387
MvaI CCWGG 1 cut(s) 243
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 3 cut(s) 292, 579, 673
NciI CCSGG 1 cut(s) 197
NdeII GATC 7 cut(s) 238, 334, 680, 715, 903, 1168, 1317
NlaIII CATG 4 cut(s) 32, 413, 1051, 1087
NlaIV GGNNCC 2 cut(s) 79, 460
NmuCI GTSAC 3 cut(s) 976, 1004, 1073
NsiI ATGCAT 1 cut(s) 550
NspI RCATGY 3 cut(s) 32, 413, 1051
PaeI GCATGC 2 cut(s) 32, 413
PagI TCATGA 1 cut(s) 1083
PciI ACATGT 1 cut(s) 1047
PcsI WCGNNNNNNNCGW 1 cut(s) 1000
PctI GAATGC 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 869
PfeI GAWTC 3 cut(s) 983, 1087, 1127
PfoI TCCNGGA 2 cut(s) 195, 241
PkrI GCNGC 4 cut(s) 27, 374, 408, 1296
Ple19I CGATCG 1 cut(s) 683
PleI GAGTC 2 cut(s) 305, 686
PpsI GAGTC 2 cut(s) 305, 686
PscI ACATGT 1 cut(s) 1047
Psp6I CCWGG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 2 cut(s) 79, 460
PspPI GGNCC 3 cut(s) 77, 458, 1261
PvuI CGATCG 1 cut(s) 683
PvuII CAGCTG 2 cut(s) 25, 406
RsaI GTAC 3 cut(s) 350, 739, 847
RsaNI GTAC 3 cut(s) 349, 738, 846
RseI CAYNNNNRTG 4 cut(s) 33, 234, 414, 615
SacII CCGCGG 1 cut(s) 373
SaqAI TTAA 4 cut(s) 84, 465, 800, 842
SatI GCNGC 4 cut(s) 26, 373, 407, 1295
Sau3AI GATC 7 cut(s) 238, 334, 680, 715, 903, 1168, 1317
Sau96I GGNCC 3 cut(s) 77, 458, 1261
SchI GAGTC 2 cut(s) 305, 686
ScrFI CCNGG 2 cut(s) 197, 243
SfaNI GCATC 4 cut(s) 154, 535, 610, 942
SfcI CTRYAG 1 cut(s) 1101
Sfr303I CCGCGG 1 cut(s) 373
SgrBI CCGCGG 1 cut(s) 373
SinI GGWCC 2 cut(s) 77, 458
SmiMI CAYNNNNRTG 4 cut(s) 33, 234, 414, 615
SmlI CTYRAG 2 cut(s) 313, 694
SmoI CTYRAG 2 cut(s) 313, 694
SpeI ACTAGT 1 cut(s) 289
SphI GCATGC 2 cut(s) 32, 413
SsiI CCGC 7 cut(s) 4, 145, 370, 372, 375, 385, 526
SspMI CTAG 4 cut(s) 290, 578, 794, 1070
StyD4I CCNGG 2 cut(s) 195, 241
StyI CCWWGG 3 cut(s) 577, 793, 1069
TaaI ACNGT 5 cut(s) 12, 393, 647, 862, 1207
TaiI ACGT 1 cut(s) 1011
TaqI TCGA 2 cut(s) 679, 826
TauI GCSGC 1 cut(s) 375
TfiI GAWTC 3 cut(s) 983, 1087, 1127
Tru1I TTAA 4 cut(s) 84, 465, 800, 842
Tru9I TTAA 4 cut(s) 84, 465, 800, 842
TscAI CASTG 4 cut(s) 15, 396, 596, 837
TseFI GTSAC 3 cut(s) 976, 1004, 1073
TseI GCWGC 3 cut(s) 25, 406, 1294
Tsp45I GTSAC 3 cut(s) 976, 1004, 1073
TspDTI ATGAA 2 cut(s) 515, 742
TspGWI ACGGA 1 cut(s) 1164
TspRI CASTG 4 cut(s) 15, 396, 596, 837
VpaK11BI GGWCC 2 cut(s) 77, 458
XapI RAATTY 2 cut(s) 188, 998
XceI RCATGY 3 cut(s) 32, 413, 1051
XmaJI CCTAGG 3 cut(s) 577, 793, 1069
XmnI GAANNNNTTC 1 cut(s) 869
XspI CTAG 4 cut(s) 290, 578, 794, 1070
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.