RH5DG571700

No description available

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0018194
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG571700.1

Sequence Viewer

Length: 534 bp
ATGCGGACACTGTTTGTAACTCAGCTGCATGCAAGTGTGAGCGAGAAGAGGCTCAAATCGGTATTTGAGAAATATGGTCCCGTTAAAAGGACGAAAATTATTCCTTACCTGTGGAAGATGAATTGTGCATTTGTGGAGTTCGAGTGGGAGAGTGATAGTGAAGATGCATTGGTGGATATGCGTTTCAAAAGCTTCCTAGGCAAATGGCAGTGGAAAGTGCAATATGCTCACTCGGATGATGCTGGAAAGGGTGTTGAAGAAGAGTGTGTTGAAGAAGTTTATTGGCTACTTGTGCCTCGATCGTCATCCGACTCAAGAAAGGCTCTTGAAGATGATCATTCCTCATCCCACTCTGGGGTCACACCAACATCAACCACTTCTGCTCCCTCATCATCCTCTGTGGATTTACCTCACTCAAGTCCACCCACAAGCCGCACAACTTTCCCAGCTCACATCAAAGTTCCCGAAGTTTTCTCAGACACCCTGACTTTTGGAACAGTTGGGGGTATCCTTCATAGCATTAGCTGCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

19.81

Weight (kDa)

5.96

Isoelectric Point (pI)

52.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 4 - 68 1.4e-10 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018194)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0083151
rosa_laevigata RLG00000037044
rosa_multiflora Rmu_sc0001287.1_g000002 Rmu_sc0002184.1_g000018
rosa_roxburghii Rroxscaffold_1G00000900
rosa_rugosa Rorug05G0440000
rosa_samantha Rh5AG532300 Rh5BG563600 Rh5CG585700 Rh5DG571700
rosa_wichuraiana Rw5G050070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 4, 433
AfiI CCNNNNNNNGG 3 cut(s) 87, 354, 355
AgsI TTSAA 4 cut(s) 187, 257, 272, 329
AluBI AGCT 4 cut(s) 25, 192, 449, 525
AluI AGCT 4 cut(s) 25, 192, 449, 525
ApeKI GCWGC 2 cut(s) 25, 525
AspA2I CCTAGG 1 cut(s) 196
AspS9I GGNCC 1 cut(s) 77
AvaII GGWCC 1 cut(s) 77
AvrII CCTAGG 1 cut(s) 196
BbvI GCAGC 2 cut(s) 12, 512
BciVI GTATCC 1 cut(s) 518
BclI TGATCA 1 cut(s) 334
BfaI CTAG 1 cut(s) 197
BfmI CTRYAG 1 cut(s) 526
BfuI GTATCC 1 cut(s) 518
BisI GCNGC 3 cut(s) 26, 433, 526
BlnI CCTAGG 1 cut(s) 196
BlsI GCNGC 3 cut(s) 27, 434, 527
Bme18I GGWCC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 1 cut(s) 79
BmsI GCATC 2 cut(s) 154, 229
BpuEI CTTGAG 2 cut(s) 298, 400
BsaBI GATNNNNATC 1 cut(s) 304
BsaJI CCNNGG 1 cut(s) 196
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 367, 397
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 354, 355
Bse8I GATNNNNATC 1 cut(s) 304
BseDI CCNNGG 1 cut(s) 196
BseGI GGATG 4 cut(s) 241, 305, 344, 392
BseJI GATNNNNATC 1 cut(s) 304
BseLI CCNNNNNNNGG 3 cut(s) 87, 354, 355
BseMII CTCAG 2 cut(s) 35, 489
BseXI GCAGC 2 cut(s) 12, 512
BseYI CCCAGC 1 cut(s) 445
Bsh1285I CGRYCG 1 cut(s) 302
BsiEI CGRYCG 1 cut(s) 302
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 3 cut(s) 87, 354, 355
BsmFI GGGAC 1 cut(s) 63
Bsp143I GATC 2 cut(s) 299, 334
BspACI CCGC 2 cut(s) 4, 433
BspCNI CTCAG 2 cut(s) 34, 488
BspLI GGNNCC 1 cut(s) 79
BspMAI CTGCAG 1 cut(s) 530
BssECI CCNNGG 1 cut(s) 196
BssMI GATC 2 cut(s) 299, 334
BssT1I CCWWGG 1 cut(s) 196
Bst4CI ACNGT 2 cut(s) 12, 499
Bst6I CTCTTC 2 cut(s) 41, 255
BstAPI GCANNNNNTGC 1 cut(s) 525
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 2 cut(s) 21, 475
BstF5I GGATG 4 cut(s) 241, 305, 344, 392
BstKTI GATC 2 cut(s) 302, 337
BstMBI GATC 2 cut(s) 299, 334
BstMCI CGRYCG 1 cut(s) 302
BstMWI GCNNNNNNNGC 3 cut(s) 198, 292, 525
BstNSI RCATGY 1 cut(s) 32
BstSFI CTRYAG 1 cut(s) 526
BstV1I GCAGC 2 cut(s) 12, 512
BsuI GTATCC 1 cut(s) 518
BtsCI GGATG 4 cut(s) 241, 305, 344, 392
BtsI GCAGTG 1 cut(s) 215
BtsIMutI CAGTG 2 cut(s) 8, 215
Cac8I GCNNGC 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 77
CviAII CATG 1 cut(s) 29
CviJI RGCY 8 cut(s) 25, 52, 192, 286, 323, 432, 449, 525
CviKI_1 RGCY 8 cut(s) 25, 52, 192, 286, 323, 432, 449, 525
DdeI CTNAG 2 cut(s) 21, 475
DpnI GATC 2 cut(s) 301, 336
DpnII GATC 2 cut(s) 299, 334
Eam1104I CTCTTC 2 cut(s) 41, 255
EarI CTCTTC 2 cut(s) 41, 255
Eco130I CCWWGG 1 cut(s) 196
Eco47I GGWCC 1 cut(s) 77
EcoT14I CCWWGG 1 cut(s) 196
EcoT22I ATGCAT 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 196
FaeI CATG 1 cut(s) 32
FaiI YATR 5 cut(s) 30, 75, 179, 225, 516
FaqI GGGAC 1 cut(s) 63
FatI CATG 1 cut(s) 28
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 3 cut(s) 26, 433, 526
FokI GGATG 4 cut(s) 248, 292, 331, 379
Fsp4HI GCNGC 3 cut(s) 26, 433, 526
FspBI CTAG 1 cut(s) 197
GluI GCNGC 3 cut(s) 26, 433, 526
GsaI CCCAGC 1 cut(s) 449
Hin1II CATG 1 cut(s) 32
HindIII AAGCTT 1 cut(s) 190
HinfI GANTC 1 cut(s) 311
Hpy166II GTNNAC 1 cut(s) 422
Hpy188I TCNGA 3 cut(s) 235, 310, 478
Hpy188III TCNNGA 3 cut(s) 315, 326, 464
Hpy8I GTNNAC 1 cut(s) 422
HpyAV CCTTC 1 cut(s) 521
HpyCH4III ACNGT 2 cut(s) 12, 499
HpyCH4V TGCA 6 cut(s) 28, 32, 128, 167, 220, 528
HpyF10VI GCNNNNNNNGC 3 cut(s) 198, 292, 525
HpyF3I CTNAG 2 cut(s) 21, 475
Hsp92II CATG 1 cut(s) 32
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 299, 334
LmnI GCTCC 1 cut(s) 388
LpnPI CCDG 5 cut(s) 122, 228, 339, 459, 497
Lsp1109I GCAGC 2 cut(s) 12, 512
LweI GCATC 2 cut(s) 154, 229
MaeI CTAG 1 cut(s) 197
MaeIII GTNAC 2 cut(s) 16, 358
MalI GATC 2 cut(s) 301, 336
MboI GATC 2 cut(s) 299, 334
MboII GAAGA 7 cut(s) 58, 127, 173, 269, 272, 284, 341
MluCI AATT 2 cut(s) 96, 121
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 6 cut(s) 42, 306, 352, 397, 406, 420
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 2 cut(s) 84, 532
MslI CAYNNNNRTG 2 cut(s) 33, 234
MspA1I CMGCKG 1 cut(s) 25
MwoI GCNNNNNNNGC 3 cut(s) 198, 292, 525
NdeII GATC 2 cut(s) 299, 334
NlaIII CATG 1 cut(s) 32
NlaIV GGNNCC 1 cut(s) 79
NmuCI GTSAC 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 1 cut(s) 32
PaeI GCATGC 1 cut(s) 32
PkrI GCNGC 3 cut(s) 27, 434, 527
Ple19I CGATCG 1 cut(s) 302
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
PspFI CCCAGC 1 cut(s) 445
PspN4I GGNNCC 1 cut(s) 79
PspPI GGNCC 1 cut(s) 77
PstI CTGCAG 1 cut(s) 530
PvuI CGATCG 1 cut(s) 302
PvuII CAGCTG 1 cut(s) 25
RseI CAYNNNNRTG 2 cut(s) 33, 234
SaqAI TTAA 2 cut(s) 84, 532
SatI GCNGC 3 cut(s) 26, 433, 526
Sau3AI GATC 2 cut(s) 299, 334
Sau96I GGNCC 1 cut(s) 77
SchI GAGTC 1 cut(s) 305
SetI ASST 6 cut(s) 27, 111, 194, 412, 451, 527
SfaNI GCATC 2 cut(s) 154, 229
SfcI CTRYAG 1 cut(s) 526
SinI GGWCC 1 cut(s) 77
SmiMI CAYNNNNRTG 2 cut(s) 33, 234
SmlI CTYRAG 2 cut(s) 313, 415
SmoI CTYRAG 2 cut(s) 313, 415
SphI GCATGC 1 cut(s) 32
Sse9I AATT 2 cut(s) 96, 121
SsiI CCGC 2 cut(s) 4, 433
SspMI CTAG 1 cut(s) 197
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 2 cut(s) 12, 499
TaqI TCGA 2 cut(s) 141, 298
TasI AATT 2 cut(s) 96, 121
TauI GCSGC 1 cut(s) 435
Tru1I TTAA 2 cut(s) 84, 532
Tru9I TTAA 2 cut(s) 84, 532
TscAI CASTG 2 cut(s) 15, 215
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 2 cut(s) 25, 525
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 2 cut(s) 134, 503
TspRI CASTG 2 cut(s) 15, 215
VpaK11BI GGWCC 1 cut(s) 77
XceI RCATGY 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 196
XspI CTAG 1 cut(s) 197
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.