Rroxscaffold_1G00024000

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0019073
Physical Location & Seq
Reverse (-)
29711343 .. 29719942
8600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00024000.1

Sequence Viewer

Length: 459 bp
ATGCAAGTAAAATTACTTGCACTCGGTGTTAATGATCACCGTCTTCCTTTTCCGGCCACTCCGACAAGAAATTCGTCACTAACATCGTCATTAGATTCACCCTCGCGGAAGGGACACGACCTCGGCGGCTTTGACCTGGTTCTTCGGCTAATCTCAAACCATCCAAACATCGAAAGCGAAATTCTCAAGGAGATCAATGAGAAAAGGGAGACCGGAGCCGGATATGATGAAGCGAAGGACATGAGGCTGCACCGCTGGTTCCGACGGACAGAAAGAGGCGGTAGATGGAGCGGATCCGGGGCAAGAATTGGCCGAGTTCCAACCCGGGAGGTGCTTGGAGTGTGGTGTGGCGGCCGAGACGTGGTGGTACTCGTTTATCTACCGGTGTTTCGGGCGGGGCTAAGGATCTGTTTGGGGAAAGGAAATGGCATTTTTGCGGATGAAAAGGGTGTTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

152

Amino Acids

16.84

Weight (kDa)

9.92

Isoelectric Point (pI)

53.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019073)

Species Orthologous Gene IDs
rosa_laevigata RLG00000015938 RLG00000022485 RLG00000033286
rosa_multiflora Rmu_sc0004239.1_g000014
rosa_roxburghii Rroxscaffold_1G00024000
rosa_rugosa Rorug02G0287100
rosa_samantha Rh2BG292900 Rh2DG287800 Rh5DG009600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 291
AccII CGCG 1 cut(s) 106
AciI CCGC 8 cut(s) 106, 126, 253, 279, 291, 351, 395, 437
AclWI GGATC 3 cut(s) 288, 301, 413
AcoI YGGCCR 3 cut(s) 54, 310, 352
AcsI RAATTY 2 cut(s) 70, 180
AdeI CACNNNGTG 1 cut(s) 26
AfaI GTAC 1 cut(s) 369
AfiI CCNNNNNNNGG 1 cut(s) 361
AgeI ACCGGT 1 cut(s) 382
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 1 cut(s) 135
Alw26I GTCTC 2 cut(s) 203, 351
AlwI GGATC 3 cut(s) 288, 301, 413
Ama87I CYCGRG 1 cut(s) 324
AoxI GGCC 3 cut(s) 54, 310, 352
ApeKI GCWGC 1 cut(s) 247
ApoI RAATTY 2 cut(s) 70, 180
ArsI GACNNNNNNTTYG 2 cut(s) 55, 87
AsiGI ACCGGT 1 cut(s) 382
AsuC2I CCSGG 3 cut(s) 298, 325, 326
AsuHPI GGTGA 2 cut(s) 29, 90
AvaI CYCGRG 1 cut(s) 324
BamHI GGATCC 1 cut(s) 293
BbsI GAAGAC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 234
BccI CCATC 2 cut(s) 168, 279
BciT130I CCWGG 1 cut(s) 137
BclI TGATCA 1 cut(s) 34
BcnI CCSGG 3 cut(s) 298, 325, 326
BcoDI GTCTC 2 cut(s) 203, 351
BisI GCNGC 3 cut(s) 127, 248, 352
BlsI GCNGC 3 cut(s) 128, 249, 353
Bme1390I CCNGG 4 cut(s) 137, 298, 325, 326
BmeT110I CYCGRG 1 cut(s) 324
BmgBI CACGTC 1 cut(s) 361
BmiI GGNNCC 3 cut(s) 217, 260, 295
BmrFI CCNGG 4 cut(s) 137, 298, 325, 326
BpiI GAAGAC 1 cut(s) 35
Bpu10I CCTNAGC 1 cut(s) 401
BpuEI CTTGAG 1 cut(s) 170
BpuMI CCSGG 3 cut(s) 298, 325, 326
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 3 cut(s) 121, 297, 324
BsaWI WCCGGW 2 cut(s) 212, 382
Bsc4I CCNNNNNNNGG 1 cut(s) 361
Bse118I RCCGGY 1 cut(s) 382
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 3 cut(s) 121, 297, 324
BseGI GGATG 2 cut(s) 160, 445
BseLI CCNNNNNNNGG 1 cut(s) 361
BseX3I CGGCCG 1 cut(s) 352
BseXI GCAGC 1 cut(s) 234
BsgI GTGCAG 1 cut(s) 233
Bsh1236I CGCG 1 cut(s) 106
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 3 cut(s) 56, 312, 354
BshTI ACCGGT 1 cut(s) 382
BsiEI CGRYCG 1 cut(s) 355
BsiHKCI CYCGRG 1 cut(s) 324
BsiSI CCGG 6 cut(s) 53, 213, 219, 297, 325, 383
BslFI GGGAC 1 cut(s) 126
BslI CCNNNNNNNGG 1 cut(s) 361
BsmAI GTCTC 2 cut(s) 203, 351
BsmBI CGTCTC 1 cut(s) 351
BsmFI GGGAC 1 cut(s) 126
BsnI GGCC 3 cut(s) 56, 312, 354
Bso31I GGTCTC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 324
Bsp143I GATC 4 cut(s) 34, 192, 293, 405
BspACI CCGC 8 cut(s) 106, 126, 253, 279, 291, 351, 395, 437
BspANI GGCC 3 cut(s) 56, 312, 354
BspFNI CGCG 1 cut(s) 106
BspLI GGNNCC 3 cut(s) 217, 260, 295
BspPI GGATC 3 cut(s) 288, 301, 413
BspTNI GGTCTC 1 cut(s) 203
BsrBI CCGCTC 1 cut(s) 291
BsrFI RCCGGY 1 cut(s) 382
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 3 cut(s) 121, 297, 324
BssMI GATC 4 cut(s) 34, 192, 293, 405
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 1 cut(s) 41
BstDEI CTNAG 1 cut(s) 401
BstF5I GGATG 2 cut(s) 160, 445
BstFNI CGCG 1 cut(s) 106
BstKTI GATC 4 cut(s) 37, 195, 296, 408
BstMAI GTCTC 2 cut(s) 203, 351
BstMBI GATC 4 cut(s) 34, 192, 293, 405
BstMCI CGRYCG 1 cut(s) 355
BstNI CCWGG 1 cut(s) 137
BstSCI CCNGG 4 cut(s) 135, 296, 323, 324
BstUI CGCG 1 cut(s) 106
BstV1I GCAGC 1 cut(s) 234
BstV2I GAAGAC 1 cut(s) 35
BstX2I RGATCY 2 cut(s) 293, 405
BstYI RGATCY 2 cut(s) 293, 405
BstZI CGGCCG 1 cut(s) 352
BsuRI GGCC 3 cut(s) 56, 312, 354
BtrI CACGTC 1 cut(s) 361
BtsCI GGATG 2 cut(s) 160, 445
Cfr10I RCCGGY 1 cut(s) 382
Cfr9I CCCGGG 1 cut(s) 324
CsiI ACCWGGT 1 cut(s) 135
Csp6I GTAC 1 cut(s) 368
CspAI ACCGGT 1 cut(s) 382
CviAII CATG 1 cut(s) 241
CviJI RGCY 8 cut(s) 56, 129, 148, 218, 247, 312, 354, 400
CviKI_1 RGCY 8 cut(s) 56, 129, 148, 218, 247, 312, 354, 400
CviQI GTAC 1 cut(s) 368
DdeI CTNAG 1 cut(s) 401
DpnI GATC 4 cut(s) 36, 194, 295, 407
DpnII GATC 4 cut(s) 34, 192, 293, 405
DraIII CACNNNGTG 1 cut(s) 26
EaeI YGGCCR 3 cut(s) 54, 310, 352
EagI CGGCCG 1 cut(s) 352
EclXI CGGCCG 1 cut(s) 352
Eco31I GGTCTC 1 cut(s) 203
Eco52I CGGCCG 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 135
Esp3I CGTCTC 1 cut(s) 351
FaeI CATG 1 cut(s) 244
FaiI YATR 2 cut(s) 225, 242
FaqI GGGAC 1 cut(s) 126
FatI CATG 1 cut(s) 240
FauI CCCGC 1 cut(s) 388
FbaI TGATCA 1 cut(s) 34
Fnu4HI GCNGC 3 cut(s) 127, 248, 352
FokI GGATG 2 cut(s) 147, 452
Fsp4HI GCNGC 3 cut(s) 127, 248, 352
GluI GCNGC 3 cut(s) 127, 248, 352
HaeIII GGCC 3 cut(s) 56, 312, 354
HapII CCGG 6 cut(s) 53, 213, 219, 297, 325, 383
Hin1II CATG 1 cut(s) 244
HinfI GANTC 1 cut(s) 95
HpaII CCGG 6 cut(s) 53, 213, 219, 297, 325, 383
HphI GGTGA 2 cut(s) 29, 90
Hpy188I TCNGA 2 cut(s) 63, 263
Hpy99I CGWCG 1 cut(s) 267
HpyAV CCTTC 2 cut(s) 103, 229
HpyCH4III ACNGT 1 cut(s) 41
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 3 cut(s) 4, 20, 250
HpyF3I CTNAG 1 cut(s) 401
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 34
Kzo9I GATC 4 cut(s) 34, 192, 293, 405
LmnI GCTCC 2 cut(s) 215, 288
LpnPI CCDG 9 cut(s) 66, 122, 149, 226, 232, 241, 310, 338, 396
Lsp1109I GCAGC 1 cut(s) 234
MabI ACCWGGT 1 cut(s) 135
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 75
MalI GATC 4 cut(s) 36, 194, 295, 407
MbiI CCGCTC 1 cut(s) 291
MboI GATC 4 cut(s) 34, 192, 293, 405
MboII GAAGA 2 cut(s) 35, 134
MflI RGATCY 2 cut(s) 293, 405
MluCI AATT 4 cut(s) 11, 70, 180, 306
MmeI TCCRAC 3 cut(s) 86, 286, 344
MnlI CCTC 5 cut(s) 112, 131, 237, 269, 322
MseI TTAA 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 255
MspI CCGG 6 cut(s) 53, 213, 219, 297, 325, 383
MspR9I CCNGG 4 cut(s) 137, 298, 325, 326
MvaI CCWGG 1 cut(s) 137
MvnI CGCG 1 cut(s) 106
NciI CCSGG 3 cut(s) 298, 325, 326
NdeII GATC 4 cut(s) 34, 192, 293, 405
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 3 cut(s) 217, 260, 295
NmeAIII GCCGAG 3 cut(s) 102, 338, 380
NmuCI GTSAC 1 cut(s) 75
PfeI GAWTC 1 cut(s) 95
PinAI ACCGGT 1 cut(s) 382
PkrI GCNGC 3 cut(s) 128, 249, 353
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 3 cut(s) 217, 260, 295
PsuI RGATCY 2 cut(s) 293, 405
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 3 cut(s) 127, 248, 352
Sau3AI GATC 4 cut(s) 34, 192, 293, 405
ScrFI CCNGG 4 cut(s) 137, 298, 325, 326
SetI ASST 4 cut(s) 123, 138, 333, 363
SexAI ACCWGGT 1 cut(s) 135
SmaI CCCGGG 1 cut(s) 326
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 4 cut(s) 11, 70, 180, 306
SsiI CCGC 8 cut(s) 106, 126, 253, 279, 291, 351, 395, 437
StyD4I CCNGG 4 cut(s) 135, 296, 323, 324
TaaI ACNGT 1 cut(s) 41
TaiI ACGT 1 cut(s) 363
TaqI TCGA 1 cut(s) 171
TasI AATT 4 cut(s) 11, 70, 180, 306
TauI GCSGC 2 cut(s) 129, 354
TfiI GAWTC 1 cut(s) 95
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TseFI GTSAC 1 cut(s) 75
TseI GCWGC 1 cut(s) 247
Tsp45I GTSAC 1 cut(s) 75
TspDTI ATGAA 2 cut(s) 243, 456
TspGWI ACGGA 1 cut(s) 280
TspMI CCCGGG 1 cut(s) 324
XapI RAATTY 2 cut(s) 70, 180
XmaI CCCGGG 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.