Rh5DG009600

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0019073
Physical Location & Seq
Forward (+)
608306 .. 608704
399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG009600.1

Sequence Viewer

Length: 399 bp
ATGGACGAGATGAAGACCCTGGAGTCTCACAATCAGCTGTCTCGGTTCTTGAGCTTCGGCCACGCCGACGAGAAATTAGTCACTGACATCGTCATCAGCTTCACCCTCGCCGGAAGGAACACGACCTCGGCGGCTTTGACCTGGTTCTTCTGGCTAATCTCAAACCATCCAAACATCGAAAGCGAAATTCTCAAGGAGATCAATGAGAAAACGGAGACCGGAGCCGGATATGATGAAGCGAAGGACATGAGGCTGTACCCGCCGGTTCCGACGAACAACAAAGAGGCGGTAGATGGTGCAGATCTGGGGCAAGGACTGGCCAAGTTCCAACCCGAGAGGTGGTTGGAGTGTGACGTGGTGGTTCGTGGCGAAGGACTCGTTTATCTACCCGGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

132

Amino Acids

14.79

Weight (kDa)

4.71

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 27 - 83 2.7e-07 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019073)

Species Orthologous Gene IDs
rosa_laevigata RLG00000015938 RLG00000022485 RLG00000033286
rosa_multiflora Rmu_sc0004239.1_g000014
rosa_roxburghii Rroxscaffold_1G00024000
rosa_rugosa Rorug02G0287100
rosa_samantha Rh2BG292900 Rh2DG287800 Rh5DG009600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 22
AciI CCGC 3 cut(s) 131, 260, 287
AcoI YGGCCR 2 cut(s) 58, 318
AcsI RAATTY 1 cut(s) 186
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 1 cut(s) 339
AjiI CACGTC 1 cut(s) 355
AjnI CCWGG 2 cut(s) 18, 140
AluBI AGCT 3 cut(s) 37, 54, 99
AluI AGCT 3 cut(s) 37, 54, 99
Alw26I GTCTC 3 cut(s) 30, 45, 209
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 2 cut(s) 58, 318
ApoI RAATTY 1 cut(s) 186
AsuC2I CCSGG 1 cut(s) 390
AsuHPI GGTGA 1 cut(s) 94
AvaI CYCGRG 1 cut(s) 332
BalI TGGCCA 1 cut(s) 320
BbsI GAAGAC 1 cut(s) 20
BccI CCATC 2 cut(s) 174, 287
BciT130I CCWGG 2 cut(s) 20, 142
BcnI CCSGG 1 cut(s) 390
BcoDI GTCTC 3 cut(s) 30, 45, 209
BglII AGATCT 1 cut(s) 301
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 3 cut(s) 20, 142, 390
BmeT110I CYCGRG 1 cut(s) 332
BmgBI CACGTC 1 cut(s) 355
BmiI GGNNCC 2 cut(s) 223, 267
BmrFI CCNGG 3 cut(s) 20, 142, 390
BpiI GAAGAC 1 cut(s) 20
BpmI CTGGAG 1 cut(s) 41
BpuEI CTTGAG 2 cut(s) 70, 176
BpuMI CCSGG 1 cut(s) 390
BsaI GGTCTC 1 cut(s) 209
BsaJI CCNNGG 2 cut(s) 18, 126
BsaWI WCCGGW 1 cut(s) 218
Bsc4I CCNNNNNNNGG 1 cut(s) 339
Bse118I RCCGGY 1 cut(s) 262
Bse1I ACTGG 1 cut(s) 321
BseBI CCWGG 2 cut(s) 20, 142
BseDI CCNNGG 2 cut(s) 18, 126
BseGI GGATG 1 cut(s) 166
BseLI CCNNNNNNNGG 1 cut(s) 339
BseNI ACTGG 1 cut(s) 321
BsgI GTGCAG 1 cut(s) 318
BshFI GGCC 2 cut(s) 60, 320
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 5 cut(s) 111, 219, 225, 263, 390
BslI CCNNNNNNNGG 1 cut(s) 339
BsmAI GTCTC 3 cut(s) 30, 45, 209
BsnI GGCC 2 cut(s) 60, 320
Bso31I GGTCTC 1 cut(s) 209
BsoBI CYCGRG 1 cut(s) 332
Bsp143I GATC 2 cut(s) 198, 301
BspACI CCGC 3 cut(s) 131, 260, 287
BspANI GGCC 2 cut(s) 60, 320
BspLI GGNNCC 2 cut(s) 223, 267
BspTNI GGTCTC 1 cut(s) 209
BsrFI RCCGGY 1 cut(s) 262
BsrI ACTGG 1 cut(s) 321
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 2 cut(s) 18, 126
BssMI GATC 2 cut(s) 198, 301
Bst2UI CCWGG 2 cut(s) 20, 142
BstF5I GGATG 1 cut(s) 166
BstKTI GATC 2 cut(s) 201, 304
BstMAI GTCTC 3 cut(s) 30, 45, 209
BstMBI GATC 2 cut(s) 198, 301
BstMWI GCNNNNNNNGC 1 cut(s) 259
BstNI CCWGG 2 cut(s) 20, 142
BstSCI CCNGG 3 cut(s) 18, 140, 388
BstV2I GAAGAC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BsuRI GGCC 2 cut(s) 60, 320
BtrI CACGTC 1 cut(s) 355
BtsCI GGATG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 81
Cfr10I RCCGGY 1 cut(s) 262
CsiI ACCWGGT 1 cut(s) 140
Csp6I GTAC 1 cut(s) 256
CviAII CATG 1 cut(s) 247
CviJI RGCY 9 cut(s) 37, 54, 60, 99, 134, 154, 224, 253, 320
CviKI_1 RGCY 9 cut(s) 37, 54, 60, 99, 134, 154, 224, 253, 320
CviQI GTAC 1 cut(s) 256
DpnI GATC 2 cut(s) 200, 303
DpnII GATC 2 cut(s) 198, 301
DrdI GACNNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 22
EaeI YGGCCR 2 cut(s) 58, 318
Eco31I GGTCTC 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 332
EcoRII CCWGG 2 cut(s) 18, 140
FaeI CATG 1 cut(s) 250
FaiI YATR 2 cut(s) 231, 248
FatI CATG 1 cut(s) 246
FauI CCCGC 1 cut(s) 267
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 1 cut(s) 153
Fsp4HI GCNGC 1 cut(s) 132
GluI GCNGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 41
HaeIII GGCC 2 cut(s) 60, 320
HapII CCGG 5 cut(s) 111, 219, 225, 263, 390
Hin1II CATG 1 cut(s) 250
HinfI GANTC 2 cut(s) 23, 375
HpaII CCGG 5 cut(s) 111, 219, 225, 263, 390
HphI GGTGA 1 cut(s) 94
Hpy188I TCNGA 1 cut(s) 270
Hpy188III TCNNGA 1 cut(s) 49
Hpy99I CGWCG 2 cut(s) 71, 274
HpyAV CCTTC 3 cut(s) 108, 235, 365
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 1 cut(s) 299
HpyF10VI GCNNNNNNNGC 1 cut(s) 259
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 1 cut(s) 250
Kzo9I GATC 2 cut(s) 198, 301
LmnI GCTCC 1 cut(s) 221
MabI ACCWGGT 1 cut(s) 140
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 2 cut(s) 79, 350
MalI GATC 2 cut(s) 200, 303
MboI GATC 2 cut(s) 198, 301
MboII GAAGA 2 cut(s) 25, 139
MflI RGATCY 1 cut(s) 301
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 2 cut(s) 74, 186
MluNI TGGCCA 1 cut(s) 320
MlyI GAGTC 2 cut(s) 32, 369
MmeI TCCRAC 3 cut(s) 293, 324, 352
MnlI CCTC 5 cut(s) 116, 136, 243, 277, 330
Mox20I TGGCCA 1 cut(s) 320
MscI TGGCCA 1 cut(s) 320
MseI TTAA 1 cut(s) 397
Msp20I TGGCCA 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 5 cut(s) 111, 219, 225, 263, 390
MspR9I CCNGG 3 cut(s) 20, 142, 390
MvaI CCWGG 2 cut(s) 20, 142
MwoI GCNNNNNNNGC 1 cut(s) 259
NciI CCSGG 1 cut(s) 390
NdeII GATC 2 cut(s) 198, 301
NlaIII CATG 1 cut(s) 250
NlaIV GGNNCC 2 cut(s) 223, 267
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 2 cut(s) 79, 350
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 2 cut(s) 31, 369
PpsI GAGTC 2 cut(s) 31, 369
Psp6I CCWGG 2 cut(s) 18, 140
PspGI CCWGG 2 cut(s) 18, 140
PspN4I GGNNCC 2 cut(s) 223, 267
PsuI RGATCY 1 cut(s) 301
PsyI GACNNNGTC 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 37
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SaqAI TTAA 1 cut(s) 397
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 2 cut(s) 198, 301
SchI GAGTC 2 cut(s) 32, 369
ScrFI CCNGG 3 cut(s) 20, 142, 390
SetI ASST 7 cut(s) 39, 56, 101, 128, 143, 341, 357
SexAI ACCWGGT 1 cut(s) 140
SmlI CTYRAG 2 cut(s) 49, 191
SmoI CTYRAG 2 cut(s) 49, 191
Sse9I AATT 2 cut(s) 74, 186
SsiI CCGC 3 cut(s) 131, 260, 287
StyD4I CCNGG 3 cut(s) 18, 140, 388
TaiI ACGT 1 cut(s) 357
TaqI TCGA 1 cut(s) 177
TasI AATT 2 cut(s) 74, 186
TauI GCSGC 1 cut(s) 134
Tru1I TTAA 1 cut(s) 397
Tru9I TTAA 1 cut(s) 397
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 2 cut(s) 79, 350
Tsp45I GTSAC 2 cut(s) 79, 350
TspDTI ATGAA 2 cut(s) 26, 249
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 1 cut(s) 88
Tth111I GACNNNGTC 1 cut(s) 89
XapI RAATTY 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.