Rroxscaffold_1G00027720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017842
Physical Location & Seq
Reverse (-)
35188427 .. 35203390
14964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00027720.1

Sequence Viewer

Length: 645 bp
ATGCTCGGCTTTGGCTCGCACCGAAGCTTAATCAAAGCTATGAGCCTAGTGATGACTATTTACAATGAAGTAGAAAGTGTCACCGAAGATGCATCGGAGAGTCGAAGACCGCCCAAAGACGGTCTCCTCTCGACGTCCTTTGTATTTTACTTGACAGGCAGCGGAAGCATTAAGAGCATCACAAATATAGATCCGCCCGGCCACCGGATCGACGTTAACAAAGAGGTACTTATTCCCGCCAAGGGAATAGCCTACGATACACCGCCGGAGAGCTATTACCGAGCATCGGATCACCCCGGATCACCGCCGACGCGCCGCCACGCGCCGTGTCAAGATGGCATCGAAGCTCCCGAGGCCGAGAACCGATGCACATCGGGCATCTTCCGCTACAACAACACACCGATAGTGGAACCCCCTTTCATCCGGCAAGCTGCGTACTTTATTCAGCCAATACCGCTTGATAAAGAACCGCCGGGGGGTCTACCGGATGTCGCTTCTCGCTACAACCGGCGGGGATATCCGCCGAGGCGGATCCCTAGGCCACGCCTCACTCTAACAACGACCGCCACGCGGCGTTATCGTCGACTCCCTACGGGACACGGGGACTTGTCAACAGCCTACGACGGCCCCGATCGTGCACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

214

Amino Acids

23.82

Weight (kDa)

9.41

Isoelectric Point (pI)

58.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 137
AccI GTMKAC 2 cut(s) 481, 583
AccII CGCG 3 cut(s) 313, 323, 571
AclWI GGATC 6 cut(s) 185, 215, 297, 307, 526, 539
AcoI YGGCCR 1 cut(s) 199
AcyI GRCGYC 1 cut(s) 134
AfaI GTAC 2 cut(s) 228, 437
AfiI CCNNNNNNNGG 6 cut(s) 119, 204, 242, 286, 476, 570
AluBI AGCT 5 cut(s) 27, 38, 273, 347, 431
AluI AGCT 5 cut(s) 27, 38, 273, 347, 431
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 1 cut(s) 128
Alw44I GTGCAC 1 cut(s) 636
AlwI GGATC 6 cut(s) 185, 215, 297, 307, 526, 539
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 4 cut(s) 199, 354, 539, 625
ApaLI GTGCAC 1 cut(s) 636
ApeKI GCWGC 2 cut(s) 159, 431
AspA2I CCTAGG 1 cut(s) 536
AspLEI GCGC 2 cut(s) 315, 325
AspS9I GGNCC 1 cut(s) 626
AsuC2I CCSGG 3 cut(s) 198, 297, 474
AsuHPI GGTGA 3 cut(s) 73, 284, 294
AvaI CYCGRG 1 cut(s) 350
AvrII CCTAGG 1 cut(s) 536
BaeGI GKGCMC 1 cut(s) 640
BamHI GGATCC 1 cut(s) 531
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 640
BbvI GCAGC 2 cut(s) 171, 418
BccI CCATC 1 cut(s) 329
BceAI ACGGC 2 cut(s) 310, 640
BcnI CCSGG 3 cut(s) 198, 297, 474
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 2 cut(s) 47, 537
BisI GCNGC 4 cut(s) 160, 316, 432, 572
BlnI CCTAGG 1 cut(s) 536
BlsI GCNGC 4 cut(s) 161, 317, 433, 573
Bme1390I CCNGG 3 cut(s) 198, 297, 474
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 626
BmiI GGNNCC 3 cut(s) 411, 533, 628
BmrFI CCNGG 3 cut(s) 198, 297, 474
BmsI GCATC 7 cut(s) 79, 101, 186, 293, 348, 356, 387
BpiI GAAGAC 1 cut(s) 112
BpuMI CCSGG 3 cut(s) 198, 297, 474
BsaBI GATNNNNATC 1 cut(s) 370
BsaHI GRCGYC 1 cut(s) 134
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 6 cut(s) 240, 295, 351, 473, 524, 536
BsaWI WCCGGW 2 cut(s) 204, 484
Bsc4I CCNNNNNNNGG 6 cut(s) 119, 204, 242, 286, 476, 570
Bse118I RCCGGY 1 cut(s) 507
Bse8I GATNNNNATC 1 cut(s) 370
BseDI CCNNGG 6 cut(s) 240, 295, 351, 473, 524, 536
BseGI GGATG 2 cut(s) 420, 493
BseJI GATNNNNATC 1 cut(s) 370
BseLI CCNNNNNNNGG 6 cut(s) 119, 204, 242, 286, 476, 570
BseRI GAGGAG 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 640
BseXI GCAGC 2 cut(s) 171, 418
Bsh1236I CGCG 3 cut(s) 313, 323, 571
Bsh1285I CGRYCG 2 cut(s) 564, 634
BshFI GGCC 4 cut(s) 201, 356, 541, 627
BsiEI CGRYCG 2 cut(s) 564, 634
BsiHKAI GWGCWC 1 cut(s) 640
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 8 cut(s) 198, 205, 266, 297, 424, 473, 485, 508
BslFI GGGAC 2 cut(s) 609, 617
BslI CCNNNNNNNGG 6 cut(s) 119, 204, 242, 286, 476, 570
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 2 cut(s) 609, 617
BsnI GGCC 4 cut(s) 201, 356, 541, 627
Bso31I GGTCTC 1 cut(s) 128
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 640
Bsp143I GATC 6 cut(s) 190, 207, 289, 299, 531, 631
BspANI GGCC 4 cut(s) 201, 356, 541, 627
BspFNI CGCG 3 cut(s) 313, 323, 571
BspLI GGNNCC 3 cut(s) 411, 533, 628
BspPI GGATC 6 cut(s) 185, 215, 297, 307, 526, 539
BspTNI GGTCTC 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 507
BssAI RCCGGY 1 cut(s) 507
BssECI CCNNGG 6 cut(s) 240, 295, 351, 473, 524, 536
BssMI GATC 6 cut(s) 190, 207, 289, 299, 531, 631
BssNI GRCGYC 1 cut(s) 134
BssT1I CCWWGG 2 cut(s) 240, 536
Bst4CI ACNGT 1 cut(s) 122
BstACI GRCGYC 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 17, 429
BstF5I GGATG 2 cut(s) 420, 493
BstFNI CGCG 3 cut(s) 313, 323, 571
BstHHI GCGC 2 cut(s) 315, 325
BstKTI GATC 6 cut(s) 193, 210, 292, 302, 534, 634
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 6 cut(s) 190, 207, 289, 299, 531, 631
BstMCI CGRYCG 2 cut(s) 564, 634
BstMWI GCNNNNNNNGC 6 cut(s) 165, 174, 353, 375, 384, 454
BstSCI CCNGG 3 cut(s) 196, 295, 472
BstSLI GKGCMC 1 cut(s) 640
BstUI CGCG 3 cut(s) 313, 323, 571
BstV1I GCAGC 2 cut(s) 171, 418
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 2 cut(s) 190, 531
BstYI RGATCY 2 cut(s) 190, 531
BsuRI GGCC 4 cut(s) 201, 356, 541, 627
BtsCI GGATG 2 cut(s) 420, 493
Cac8I GCNNGC 2 cut(s) 17, 429
CfoI GCGC 2 cut(s) 315, 325
Cfr10I RCCGGY 1 cut(s) 507
Cfr13I GGNCC 1 cut(s) 626
CseI GACGC 1 cut(s) 319
Csp6I GTAC 2 cut(s) 227, 436
CviQI GTAC 2 cut(s) 227, 436
DpnI GATC 6 cut(s) 192, 209, 291, 301, 533, 633
DpnII GATC 6 cut(s) 190, 207, 289, 299, 531, 631
EaeI YGGCCR 1 cut(s) 199
EciI GGCGGA 3 cut(s) 183, 510, 544
Eco130I CCWWGG 2 cut(s) 240, 536
Eco31I GGTCTC 1 cut(s) 128
Eco32I GATATC 1 cut(s) 518
Eco88I CYCGRG 1 cut(s) 350
EcoRV GATATC 1 cut(s) 518
EcoT14I CCWWGG 2 cut(s) 240, 536
EcoT22I ATGCAT 1 cut(s) 94
ErhI CCWWGG 2 cut(s) 240, 536
FaiI YATR 2 cut(s) 41, 188
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FaqI GGGAC 2 cut(s) 609, 617
FauI CCCGC 2 cut(s) 244, 504
FblI GTMKAC 2 cut(s) 481, 583
Fnu4HI GCNGC 4 cut(s) 160, 316, 432, 572
FokI GGATG 2 cut(s) 407, 500
Fsp4HI GCNGC 4 cut(s) 160, 316, 432, 572
FspBI CTAG 2 cut(s) 47, 537
GlaI GCGC 2 cut(s) 314, 324
GluI GCNGC 4 cut(s) 160, 316, 432, 572
HaeIII GGCC 4 cut(s) 201, 356, 541, 627
HapII CCGG 8 cut(s) 198, 205, 266, 297, 424, 473, 485, 508
HgaI GACGC 1 cut(s) 319
HhaI GCGC 2 cut(s) 315, 325
Hin1I GRCGYC 1 cut(s) 134
Hin6I GCGC 2 cut(s) 313, 323
HinP1I GCGC 2 cut(s) 313, 323
HincII GTYRAC 3 cut(s) 217, 584, 612
HindII GTYRAC 3 cut(s) 217, 584, 612
HindIII AAGCTT 1 cut(s) 25
HinfI GANTC 2 cut(s) 100, 585
HpaI GTTAAC 1 cut(s) 217
HpaII CCGG 8 cut(s) 198, 205, 266, 297, 424, 473, 485, 508
HphI GGTGA 3 cut(s) 73, 284, 294
Hpy166II GTNNAC 5 cut(s) 217, 482, 584, 612, 638
Hpy188I TCNGA 2 cut(s) 97, 289
Hpy188III TCNNGA 3 cut(s) 130, 332, 350
Hpy8I GTNNAC 5 cut(s) 217, 482, 584, 612, 638
Hpy99I CGWCG 5 cut(s) 136, 215, 313, 585, 626
HpyCH4III ACNGT 1 cut(s) 122
HpyCH4IV ACGT 3 cut(s) 134, 213, 640
HpyCH4V TGCA 3 cut(s) 92, 369, 638
HpyF10VI GCNNNNNNNGC 6 cut(s) 165, 174, 353, 375, 384, 454
HpySE526I ACGT 3 cut(s) 134, 213, 640
Hsp92I GRCGYC 1 cut(s) 134
HspAI GCGC 2 cut(s) 313, 323
KspAI GTTAAC 1 cut(s) 217
Kzo9I GATC 6 cut(s) 190, 207, 289, 299, 531, 631
LmnI GCTCC 1 cut(s) 352
LpnPI CCDG 9 cut(s) 141, 211, 218, 279, 310, 437, 486, 498, 521
Lsp1109I GCAGC 2 cut(s) 171, 418
LweI GCATC 7 cut(s) 79, 101, 186, 293, 348, 356, 387
MaeI CTAG 2 cut(s) 47, 537
MaeII ACGT 3 cut(s) 134, 213, 640
MaeIII GTNAC 1 cut(s) 79
MalI GATC 6 cut(s) 192, 209, 291, 301, 533, 633
MboI GATC 6 cut(s) 190, 207, 289, 299, 531, 631
MboII GAAGA 3 cut(s) 98, 117, 373
MflI RGATCY 2 cut(s) 190, 531
MhlI GDGCHC 1 cut(s) 640
MlyI GAGTC 2 cut(s) 109, 579
MnlI CCTC 5 cut(s) 137, 217, 346, 519, 557
Mph1103I ATGCAT 1 cut(s) 94
MseI TTAA 3 cut(s) 29, 171, 216
MspA1I CMGCKG 1 cut(s) 162
MspI CCGG 8 cut(s) 198, 205, 266, 297, 424, 473, 485, 508
MspR9I CCNGG 3 cut(s) 198, 297, 474
MvnI CGCG 3 cut(s) 313, 323, 571
MwoI GCNNNNNNNGC 6 cut(s) 165, 174, 353, 375, 384, 454
NciI CCSGG 3 cut(s) 198, 297, 474
NdeII GATC 6 cut(s) 190, 207, 289, 299, 531, 631
NlaIV GGNNCC 3 cut(s) 411, 533, 628
NmeAIII GCCGAG 2 cut(s) 382, 549
NmuCI GTSAC 1 cut(s) 79
NsiI ATGCAT 1 cut(s) 94
PcsI WCGNNNNNNNCGW 2 cut(s) 348, 627
PkrI GCNGC 4 cut(s) 161, 317, 433, 573
Ple19I CGATCG 1 cut(s) 634
PleI GAGTC 2 cut(s) 108, 579
PpsI GAGTC 2 cut(s) 108, 579
PspN4I GGNNCC 3 cut(s) 411, 533, 628
PspPI GGNCC 1 cut(s) 626
PsuI RGATCY 2 cut(s) 190, 531
PvuI CGATCG 1 cut(s) 634
RsaI GTAC 2 cut(s) 228, 437
RsaNI GTAC 2 cut(s) 227, 436
SalI GTCGAC 1 cut(s) 582
SaqAI TTAA 3 cut(s) 29, 171, 216
SatI GCNGC 4 cut(s) 160, 316, 432, 572
Sau3AI GATC 6 cut(s) 190, 207, 289, 299, 531, 631
Sau96I GGNCC 1 cut(s) 626
SchI GAGTC 2 cut(s) 109, 579
ScrFI CCNGG 3 cut(s) 198, 297, 474
SduI GDGCHC 1 cut(s) 640
SetI ASST 9 cut(s) 29, 40, 137, 216, 228, 275, 349, 433, 643
SfaNI GCATC 7 cut(s) 79, 101, 186, 293, 348, 356, 387
SspMI CTAG 2 cut(s) 47, 537
StyD4I CCNGG 3 cut(s) 196, 295, 472
StyI CCWWGG 2 cut(s) 240, 536
TaaI ACNGT 1 cut(s) 122
TaiI ACGT 3 cut(s) 137, 216, 643
TaqI TCGA 5 cut(s) 103, 131, 210, 342, 583
TauI GCSGC 2 cut(s) 318, 574
Tru1I TTAA 3 cut(s) 29, 171, 216
Tru9I TTAA 3 cut(s) 29, 171, 216
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 2 cut(s) 159, 431
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 81, 409
VneI GTGCAC 1 cut(s) 636
XmaJI CCTAGG 1 cut(s) 536
XmiI GTMKAC 2 cut(s) 481, 583
XspI CTAG 2 cut(s) 47, 537
ZraI GACGTC 1 cut(s) 135
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.