Rroxscaffold_1G00041950

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017640
Physical Location & Seq
Forward (+)
59957683 .. 59962805
5123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00041950.1

Sequence Viewer

Length: 360 bp
ATGAACTGGAGGAGGGGTAAAATCGACAATCCCGAATATGCCAAATTGCGTAAAAAGGGAATTAACCCTAAATTGGAAGAGAAGCTCGGATATGATGTTCCAACAAGTTACCGCAAGTGGTGTTCATGCTTGGGCACCATCATCCGGTGGTACCAAATATCCCACTTTGATGTTCCAACCGTAACCGGTGGCTACATTGATTTGGACATACTAGTAGAGTTTGGTGGTGGGCATAAGAAGTTTGGCATTACAAGACTTCACATGGAAGATGATGCAAGGAAGCTTATTGATTCAGTCGACGAAGAAGTTACTCGTAGGAAAGAACCCATATTTCCTTGCAATAATATTGTTAGAACATGA

Protein Analysis

119

Amino Acids

13.9

Weight (kDa)

9.01

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GatB_N PF02934 51 - 101 5.5e-07 GatB/GatE catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g04730
rosa_chinensis RchiOBHm_Chr4g0395921
rosa_laevigata RLG00000022853
rosa_roxburghii Rroxscaffold_1G00041950 Rroxscaffold_1G00043970
rosa_rugosa Rorug07G0205900
rosa_samantha Rh4BG062700 Rh4CG069000 Rh7AG206700 Rh7BG206200 Rh7BG337300 Rh7DG213900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 150
AccB1I GGYRCC 2 cut(s) 134, 150
AccI GTMKAC 1 cut(s) 297
AciI CCGC 1 cut(s) 112
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 2 cut(s) 73, 144
AgeI ACCGGT 1 cut(s) 185
AhlI ACTAGT 1 cut(s) 211
AloI GAACNNNNNNTCC 2 cut(s) 81, 113
AluBI AGCT 2 cut(s) 85, 283
AluI AGCT 2 cut(s) 85, 283
AsiGI ACCGGT 1 cut(s) 185
Asp718I GGTACC 1 cut(s) 150
BaeGI GKGCMC 1 cut(s) 137
BanI GGYRCC 2 cut(s) 134, 150
BccI CCATC 1 cut(s) 146
BcuI ACTAGT 1 cut(s) 211
BfaI CTAG 1 cut(s) 212
BmiI GGNNCC 2 cut(s) 136, 152
BmsI GCATC 1 cut(s) 262
BpmI CTGGAG 1 cut(s) 28
BsaWI WCCGGW 2 cut(s) 144, 185
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 144
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 1 cut(s) 11
BseGI GGATG 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 73, 144
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 1 cut(s) 25
BseSI GKGCMC 1 cut(s) 137
BshNI GGYRCC 2 cut(s) 134, 150
BshTI ACCGGT 1 cut(s) 185
BsiSI CCGG 2 cut(s) 145, 186
BslI CCNNNNNNNGG 2 cut(s) 73, 144
Bsp1286I GDGCHC 1 cut(s) 137
BspACI CCGC 1 cut(s) 112
BspLI GGNNCC 2 cut(s) 136, 152
BspT107I GGYRCC 2 cut(s) 134, 150
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 185
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 1 cut(s) 72
BstF5I GGATG 1 cut(s) 141
BstSLI GKGCMC 1 cut(s) 137
BtsCI GGATG 1 cut(s) 141
Cfr10I RCCGGY 1 cut(s) 185
Csp6I GTAC 1 cut(s) 151
CspAI ACCGGT 1 cut(s) 185
CviAII CATG 3 cut(s) 126, 262, 357
CviJI RGCY 3 cut(s) 85, 192, 283
CviKI_1 RGCY 3 cut(s) 85, 192, 283
CviQI GTAC 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
FaeI CATG 3 cut(s) 129, 265, 360
FaiI YATR 8 cut(s) 39, 93, 127, 209, 234, 263, 329, 358
FatI CATG 3 cut(s) 125, 261, 356
FblI GTMKAC 1 cut(s) 297
FokI GGATG 1 cut(s) 128
FspBI CTAG 1 cut(s) 212
GsuI CTGGAG 1 cut(s) 28
HapII CCGG 2 cut(s) 145, 186
Hin1II CATG 3 cut(s) 129, 265, 360
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 1 cut(s) 290
HpaII CCGG 2 cut(s) 145, 186
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 1 cut(s) 32
Hpy8I GTNNAC 1 cut(s) 298
Hpy99I CGWCG 1 cut(s) 302
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4V TGCA 2 cut(s) 275, 339
Hsp92II CATG 3 cut(s) 129, 265, 360
KpnI GGTACC 1 cut(s) 154
LpnPI CCDG 2 cut(s) 158, 199
LweI GCATC 1 cut(s) 262
MaeI CTAG 1 cut(s) 212
MaeIII GTNAC 3 cut(s) 107, 181, 307
MboII GAAGA 3 cut(s) 89, 278, 314
MhlI GDGCHC 1 cut(s) 137
MluCI AATT 3 cut(s) 44, 60, 71
MmeI TCCRAC 2 cut(s) 125, 200
MnlI CCTC 2 cut(s) 3, 6
MseI TTAA 1 cut(s) 63
MslI CAYNNNNRTG 1 cut(s) 168
MspI CCGG 2 cut(s) 145, 186
NlaIII CATG 3 cut(s) 129, 265, 360
NlaIV GGNNCC 2 cut(s) 136, 152
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PfeI GAWTC 1 cut(s) 290
PinAI ACCGGT 1 cut(s) 185
PspN4I GGNNCC 2 cut(s) 136, 152
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 168
SalI GTCGAC 1 cut(s) 296
SaqAI TTAA 1 cut(s) 63
SduI GDGCHC 1 cut(s) 137
SetI ASST 2 cut(s) 87, 285
SfaNI GCATC 1 cut(s) 262
SmiMI CAYNNNNRTG 1 cut(s) 168
SpeI ACTAGT 1 cut(s) 211
Sse9I AATT 3 cut(s) 44, 60, 71
SsiI CCGC 1 cut(s) 112
SspI AATATT 1 cut(s) 346
SspMI CTAG 1 cut(s) 212
TaaI ACNGT 1 cut(s) 181
TaqI TCGA 2 cut(s) 24, 297
TasI AATT 3 cut(s) 44, 60, 71
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TspDTI ATGAA 2 cut(s) 17, 114
XmiI GTMKAC 1 cut(s) 297
XspI CTAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.