Rroxscaffold_1G00048380

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007273
Physical Location & Seq
Forward (+)
68345191 .. 68349405
4215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00048380.1

Sequence Viewer

Length: 1647 bp
ATGGGGCAAAGAAATATGCTATGCCCTAGTCAGATGATGGATTTGGAAATGTATCAACAAGGTGAAGCTTATCTCTGTCCGCCACCCTCCATCCATTTTGGCGGCTCAACCAGTATCCAACAACCTAATATTCACACAATGGTAACAGCTTCGGGGAGCAGTACTAATTTTGACACCCAACATTTACCCGAGCGTTATGATAGTGCCCCATTCTACGGAATGACCCAGTACAACGGTCAGCATCATCATAATCTTGATTTGGGTGTTGCAACTCCAGCCAACTTTTATTATTCCTACATGACACCTTCATCTAGCTCTGGGGTATTACCTGTTCCTATGAATCATGGGGCCTCTGATTCGCTGCCTTCTTCTAGCAATTATGGAGTCGTCGGAATTTCTGCAGATGAGTATGGAAGGAATAGTCGCATCATGGATGATCTCAGAGGTCCATGCAAGAGAAAGAATCCTGAATGCCTGCCAGGGAATTTCCAATATTTCAATGCCTCTGCAAGTCCTAGTACTTCTGTGCCTCCTTTGAATATGAGGCATCCTGAAGGGGTAGCTGTAATGGATGCTGTAAACTTTGCCCTACCTCCTCAGTTTGTAGGAACTGGCAATCCACCAATTATGGAAGCTGGACCTCAGATTAGCATGAGGAATCGTTCAGCTACAACTGGGTTGGAATCTGTACCAACACATGACCACAACCATCTGAGTCAAGGAAACTATGTCGGTCAGCGCTTTCAGCCGGCTGGAACACTCTGGTTGGACCAACATTTAAGTAGTAATAGTGGAGATGGAGGAGCTTCAGCCTGGAATCAAGTTCCTATTCCTTTTATGCATGGTGGCAATGTCGGCGGAGGTTCCATGGACAGTGTCAACGTGGGTATGCAAAGATATCATGATTCATCTAGCAACAGAAGTTCCTCTATTCTCCGGCATCCTCCTCCTCTCAACCACCGGCATCAAAATCATCATGTAGCTCCACCACCCATGCAAGGATTGAGAGGCCACAACCTGAATCTTCCCCAAGTAGCCACAGAACCTTCATATAGACTACCCTCAAGTTCCTCACGCAGCTCCACCAGGAATCCATCTCAGAATGGTCTGGAGATTGGTCGTAGGCAACCAGGACCCATTCCACAAACCGGCTTTAGGATATACAGGCCTCACCAAGGAGTCATTTCTGAGACAGCTCTAAGACACCACAACCTTGCGCATTGGAGAGTTCTTGGGGTTGATGAGGGTGTCATACTAGATGTTCCAGAATTTGGCAACTTTGGTGACCAGCACAGAGATATGCGGTTGGACATAGAGGATATGTCCTATGAGGAGCTTCTTGCATTGGGGGAACAAATTGGCCATGTAAACACTGGGTTATCGGAGGACAGCATTAGCAAACAACTAAAGACAAGGAGTTATTTATCATCTGGTACAGTTATAAATCTGGAAGAAGCTGGATGTCCAGAGAAGGAAGTTGATTTTTGCATAATATGCCAGGATTATTATAAGAACCAGGAGAAGATAGGGACTCTTCATTGTGGACATGAGTATCATGCAGATTGCTTAAAGAAGTGGTTGCTTGTGAAAAATGTCTGCCCCATCTGCAAATCCGAAGCACTGATCACAGGGAAGAAGGATGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

548

Amino Acids

60.21

Weight (kDa)

6.28

Isoelectric Point (pI)

54.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 488 - 537 4.8e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 496 - 537 2.1e-11 Ring finger domain
zf-C3HC4_2 PF13923 496 - 536 2e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 496 - 536 2.1e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1442, 1509
Acc16I TGCGCA 1 cut(s) 1218
AccB7I CCANNNNNTGG 1 cut(s) 1271
AciI CCGC 4 cut(s) 80, 102, 858, 1303
AcoI YGGCCR 1 cut(s) 1360
AcsI RAATTY 3 cut(s) 393, 484, 1268
AcuI CTGAAG 2 cut(s) 573, 792
AfaI GTAC 5 cut(s) 163, 230, 520, 690, 1435
AfeI AGCGCT 1 cut(s) 740
AfiI CCNNNNNNNGG 6 cut(s) 215, 998, 1148, 1155, 1175, 1271
AgsI TTSAA 2 cut(s) 499, 538
AjnI CCWGG 6 cut(s) 478, 812, 1085, 1129, 1497, 1515
AloI GAACNNNNNNTCC 2 cut(s) 601, 633
Alw26I GTCTC 1 cut(s) 1184
Ama87I CYCGRG 1 cut(s) 188
Aor51HI AGCGCT 1 cut(s) 740
AoxI GGCC 4 cut(s) 348, 1009, 1166, 1360
ApeKI GCWGC 2 cut(s) 361, 1077
ApoI RAATTY 3 cut(s) 393, 484, 1268
AspLEI GCGC 2 cut(s) 741, 1219
AspS9I GGNCC 5 cut(s) 348, 446, 638, 769, 1133
AsuHPI GGTGA 3 cut(s) 74, 1163, 1295
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 4 cut(s) 446, 638, 769, 1133
BaeGI GKGCMC 1 cut(s) 208
BaeI ACNNNNGTAYC 2 cut(s) 1535, 1568
BalI TGGCCA 1 cut(s) 1362
BbvI GCAGC 2 cut(s) 348, 1089
BccI CCATC 6 cut(s) 31, 98, 717, 791, 1102, 1610
BciT130I CCWGG 6 cut(s) 480, 814, 1087, 1131, 1499, 1517
BciVI GTATCC 1 cut(s) 125
BclI TGATCA 1 cut(s) 1623
BcoDI GTCTC 1 cut(s) 1184
BfaI CTAG 6 cut(s) 27, 312, 372, 516, 912, 1256
BfmI CTRYAG 1 cut(s) 399
BfoI RGCGCY 1 cut(s) 742
BfuI GTATCC 1 cut(s) 125
BisI GCNGC 3 cut(s) 103, 362, 1078
BlsI GCNGC 3 cut(s) 104, 363, 1079
BmcAI AGTACT 2 cut(s) 163, 520
Bme1390I CCNGG 6 cut(s) 480, 814, 1087, 1131, 1499, 1517
Bme18I GGWCC 4 cut(s) 446, 638, 769, 1133
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 5 cut(s) 348, 446, 638, 769, 1133
BmiI GGNNCC 3 cut(s) 349, 865, 1135
BmrFI CCNGG 6 cut(s) 480, 814, 1087, 1131, 1499, 1517
BmrI ACTGGG 3 cut(s) 220, 684, 1383
BmsI GCATC 6 cut(s) 250, 435, 556, 562, 949, 973
BmuI ACTGGG 3 cut(s) 220, 684, 1383
BpmI CTGGAG 2 cut(s) 258, 1130
BpuEI CTTGAG 1 cut(s) 1048
BsaJI CCNNGG 3 cut(s) 479, 867, 1174
Bsc4I CCNNNNNNNGG 6 cut(s) 215, 998, 1148, 1155, 1175, 1271
Bse118I RCCGGY 3 cut(s) 748, 960, 1148
Bse1I ACTGG 5 cut(s) 111, 226, 616, 679, 1378
Bse3DI GCAATG 1 cut(s) 856
BseBI CCWGG 6 cut(s) 480, 814, 1087, 1131, 1499, 1517
BseDI CCNNGG 3 cut(s) 479, 867, 1174
BseGI GGATG 7 cut(s) 90, 439, 547, 577, 940, 1466, 1645
BseLI CCNNNNNNNGG 6 cut(s) 215, 998, 1148, 1155, 1175, 1271
BseMI GCAATG 1 cut(s) 856
BseMII CTCAG 6 cut(s) 454, 611, 656, 704, 1112, 1179
BseNI ACTGG 5 cut(s) 111, 226, 616, 679, 1378
BseRI GAGGAG 5 cut(s) 585, 816, 936, 939, 1346
BseSI GKGCMC 1 cut(s) 208
BseXI GCAGC 2 cut(s) 348, 1089
BshFI GGCC 4 cut(s) 350, 1011, 1168, 1362
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 4 cut(s) 749, 937, 961, 1149
BslFI GGGAC 1 cut(s) 1543
BslI CCNNNNNNNGG 6 cut(s) 215, 998, 1148, 1155, 1175, 1271
BsmAI GTCTC 1 cut(s) 1184
BsmFI GGGAC 1 cut(s) 1543
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 4 cut(s) 350, 1011, 1168, 1362
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 2 cut(s) 436, 1623
Bsp19I CCATGG 1 cut(s) 867
BspACI CCGC 4 cut(s) 80, 102, 858, 1303
BspANI GGCC 4 cut(s) 350, 1011, 1168, 1362
BspCNI CTCAG 6 cut(s) 453, 610, 655, 705, 1111, 1180
BspHI TCATGA 1 cut(s) 901
BspLI GGNNCC 3 cut(s) 349, 865, 1135
BspMAI CTGCAG 1 cut(s) 403
BsrDI GCAATG 1 cut(s) 856
BsrFI RCCGGY 3 cut(s) 748, 960, 1148
BsrI ACTGG 5 cut(s) 111, 226, 616, 679, 1378
BssAI RCCGGY 3 cut(s) 748, 960, 1148
BssECI CCNNGG 3 cut(s) 479, 867, 1174
BssMI GATC 2 cut(s) 436, 1623
BssT1I CCWWGG 2 cut(s) 867, 1174
Bst2UI CCWGG 6 cut(s) 480, 814, 1087, 1131, 1499, 1517
Bst4CI ACNGT 3 cut(s) 236, 875, 1438
Bst6I CTCTTC 1 cut(s) 1539
BstAPI GCANNNNNTGC 1 cut(s) 1494
BstC8I GCNNGC 2 cut(s) 476, 750
BstDEI CTNAG 7 cut(s) 440, 597, 642, 713, 1098, 1188, 1199
BstDSI CCRYGG 1 cut(s) 867
BstEII GGTNACC 1 cut(s) 1283
BstENI CCTNNNNNAGG 1 cut(s) 1173
BstF5I GGATG 7 cut(s) 90, 439, 547, 577, 940, 1466, 1645
BstH2I RGCGCY 1 cut(s) 742
BstHHI GCGC 2 cut(s) 741, 1219
BstKTI GATC 2 cut(s) 439, 1626
BstMAI GTCTC 1 cut(s) 1184
BstMBI GATC 2 cut(s) 436, 1623
BstMWI GCNNNNNNNGC 5 cut(s) 275, 745, 855, 1494, 1605
BstNI CCWGG 6 cut(s) 480, 814, 1087, 1131, 1499, 1517
BstPI GGTNACC 1 cut(s) 1283
BstSCI CCNGG 6 cut(s) 478, 812, 1085, 1129, 1497, 1515
BstSFI CTRYAG 1 cut(s) 399
BstSLI GKGCMC 1 cut(s) 208
BstV1I GCAGC 2 cut(s) 348, 1089
BsuI GTATCC 1 cut(s) 125
BsuRI GGCC 4 cut(s) 350, 1011, 1168, 1362
BtgI CCRYGG 1 cut(s) 867
BtsCI GGATG 7 cut(s) 90, 439, 547, 577, 940, 1466, 1645
BtsIMutI CAGTG 3 cut(s) 880, 1371, 1619
Cac8I GCNNGC 2 cut(s) 476, 750
CciI TCATGA 1 cut(s) 901
CfoI GCGC 2 cut(s) 741, 1219
Cfr10I RCCGGY 3 cut(s) 748, 960, 1148
Cfr13I GGNCC 5 cut(s) 348, 446, 638, 769, 1133
Csp6I GTAC 5 cut(s) 162, 229, 519, 689, 1434
CviQI GTAC 5 cut(s) 162, 229, 519, 689, 1434
DdeI CTNAG 7 cut(s) 440, 597, 642, 713, 1098, 1188, 1199
DpnI GATC 2 cut(s) 438, 1625
DpnII GATC 2 cut(s) 436, 1623
EaeI YGGCCR 1 cut(s) 1360
Eam1104I CTCTTC 1 cut(s) 1539
EarI CTCTTC 1 cut(s) 1539
EciI GGCGGA 2 cut(s) 69, 873
Eco130I CCWWGG 2 cut(s) 867, 1174
Eco147I AGGCCT 1 cut(s) 1168
Eco32I GATATC 1 cut(s) 899
Eco47I GGWCC 4 cut(s) 446, 638, 769, 1133
Eco47III AGCGCT 1 cut(s) 740
Eco57I CTGAAG 2 cut(s) 573, 792
Eco88I CYCGRG 1 cut(s) 188
Eco91I GGTNACC 1 cut(s) 1283
EcoNI CCTNNNNNAGG 1 cut(s) 1173
EcoO109I RGGNCCY 2 cut(s) 348, 1133
EcoO65I GGTNACC 1 cut(s) 1283
EcoRII CCWGG 6 cut(s) 478, 812, 1085, 1129, 1497, 1515
EcoRV GATATC 1 cut(s) 899
EcoT14I CCWWGG 2 cut(s) 867, 1174
EcoT22I ATGCAT 1 cut(s) 843
ErhI CCWWGG 2 cut(s) 867, 1174
FaqI GGGAC 1 cut(s) 1543
FbaI TGATCA 1 cut(s) 1623
Fnu4HI GCNGC 3 cut(s) 103, 362, 1078
FokI GGATG 6 cut(s) 77, 446, 534, 584, 927, 1473
Fsp4HI GCNGC 3 cut(s) 103, 362, 1078
FspBI CTAG 6 cut(s) 27, 312, 372, 516, 912, 1256
FspI TGCGCA 1 cut(s) 1218
GlaI GCGC 2 cut(s) 740, 1218
GluI GCNGC 3 cut(s) 103, 362, 1078
GsuI CTGGAG 2 cut(s) 258, 1130
HaeII RGCGCY 1 cut(s) 742
HaeIII GGCC 4 cut(s) 350, 1011, 1168, 1362
HapII CCGG 4 cut(s) 749, 937, 961, 1149
HhaI GCGC 2 cut(s) 741, 1219
Hin6I GCGC 2 cut(s) 739, 1217
HinP1I GCGC 2 cut(s) 739, 1217
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 1 cut(s) 66
HpaII CCGG 4 cut(s) 749, 937, 961, 1149
HphI GGTGA 3 cut(s) 74, 1163, 1295
Hpy166II GTNNAC 4 cut(s) 580, 880, 1369, 1544
Hpy188III TCNNGA 8 cut(s) 254, 467, 551, 902, 1109, 1265, 1448, 1466
Hpy8I GTNNAC 4 cut(s) 580, 880, 1369, 1544
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 7 cut(s) 315, 375, 408, 548, 1056, 1465, 1630
HpyCH4III ACNGT 3 cut(s) 236, 875, 1438
HpyCH4IV ACGT 1 cut(s) 882
HpyF10VI GCNNNNNNNGC 5 cut(s) 275, 745, 855, 1494, 1605
HpyF3I CTNAG 7 cut(s) 440, 597, 642, 713, 1098, 1188, 1199
HpySE526I ACGT 1 cut(s) 882
HspAI GCGC 2 cut(s) 739, 1217
KroI GCCGGC 1 cut(s) 748
KroNI GCCGGC 1 cut(s) 750
Ksp22I TGATCA 1 cut(s) 1623
Kzo9I GATC 2 cut(s) 436, 1623
LmnI GCTCC 5 cut(s) 156, 803, 988, 1085, 1333
Lsp1109I GCAGC 2 cut(s) 348, 1089
LweI GCATC 6 cut(s) 250, 435, 556, 562, 949, 973
MaeI CTAG 6 cut(s) 27, 312, 372, 516, 912, 1256
MaeII ACGT 1 cut(s) 882
MaeIII GTNAC 2 cut(s) 142, 1283
MalI GATC 2 cut(s) 438, 1625
MboI GATC 2 cut(s) 436, 1623
MboII GAAGA 6 cut(s) 360, 1016, 1463, 1526, 1534, 1645
MhlI GDGCHC 1 cut(s) 208
MlsI TGGCCA 1 cut(s) 1362
MluCI AATT 7 cut(s) 166, 376, 393, 484, 624, 1268, 1356
MluNI TGGCCA 1 cut(s) 1362
MlyI GAGTC 4 cut(s) 393, 724, 1188, 1525
MmeI TCCRAC 5 cut(s) 142, 370, 660, 747, 1287
Mox20I TGGCCA 1 cut(s) 1362
Mph1103I ATGCAT 1 cut(s) 843
MroNI GCCGGC 1 cut(s) 748
MscI TGGCCA 1 cut(s) 1362
MseI TTAA 2 cut(s) 779, 1568
Msp20I TGGCCA 1 cut(s) 1362
MspI CCGG 4 cut(s) 749, 937, 961, 1149
MspR9I CCNGG 6 cut(s) 480, 814, 1087, 1131, 1499, 1517
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 6 cut(s) 480, 814, 1087, 1131, 1499, 1517
MwoI GCNNNNNNNGC 5 cut(s) 275, 745, 855, 1494, 1605
NaeI GCCGGC 1 cut(s) 750
NcoI CCATGG 1 cut(s) 867
NdeII GATC 2 cut(s) 436, 1623
NgoMIV GCCGGC 1 cut(s) 748
NlaIV GGNNCC 3 cut(s) 349, 865, 1135
NmuCI GTSAC 1 cut(s) 1283
NsbI TGCGCA 1 cut(s) 1218
NsiI ATGCAT 1 cut(s) 843
PagI TCATGA 1 cut(s) 901
PceI AGGCCT 1 cut(s) 1168
PctI GAATGC 1 cut(s) 476
PdiI GCCGGC 1 cut(s) 750
PfeI GAWTC 9 cut(s) 340, 356, 463, 658, 683, 817, 905, 1021, 1090
PflFI GACNNNGTC 1 cut(s) 875
PflMI CCANNNNNTGG 1 cut(s) 1271
PkrI GCNGC 3 cut(s) 104, 363, 1079
PleI GAGTC 4 cut(s) 392, 723, 1187, 1525
PpsI GAGTC 4 cut(s) 392, 723, 1187, 1525
PpuMI RGGWCCY 1 cut(s) 1133
PsiI TTATAA 2 cut(s) 1442, 1509
Psp5II RGGWCCY 1 cut(s) 1133
Psp6I CCWGG 6 cut(s) 478, 812, 1085, 1129, 1497, 1515
PspEI GGTNACC 1 cut(s) 1283
PspGI CCWGG 6 cut(s) 478, 812, 1085, 1129, 1497, 1515
PspN4I GGNNCC 3 cut(s) 349, 865, 1135
PspPI GGNCC 5 cut(s) 348, 446, 638, 769, 1133
PspPPI RGGWCCY 1 cut(s) 1133
PsrI GAACNNNNNNTAC 2 cut(s) 315, 347
PstI CTGCAG 1 cut(s) 403
PsyI GACNNNGTC 1 cut(s) 875
RsaI GTAC 5 cut(s) 163, 230, 520, 690, 1435
RsaNI GTAC 5 cut(s) 162, 229, 519, 689, 1434
SaqAI TTAA 2 cut(s) 779, 1568
SatI GCNGC 3 cut(s) 103, 362, 1078
Sau3AI GATC 2 cut(s) 436, 1623
Sau96I GGNCC 5 cut(s) 348, 446, 638, 769, 1133
ScaI AGTACT 2 cut(s) 163, 520
SchI GAGTC 4 cut(s) 393, 724, 1188, 1525
ScrFI CCNGG 6 cut(s) 480, 814, 1087, 1131, 1499, 1517
SduI GDGCHC 1 cut(s) 208
SfaNI GCATC 6 cut(s) 250, 435, 556, 562, 949, 973
SfcI CTRYAG 1 cut(s) 399
SinI GGWCC 4 cut(s) 446, 638, 769, 1133
SmlI CTYRAG 1 cut(s) 1063
SmoI CTYRAG 1 cut(s) 1063
Sse9I AATT 7 cut(s) 166, 376, 393, 484, 624, 1268, 1356
SseBI AGGCCT 1 cut(s) 1168
SsiI CCGC 4 cut(s) 80, 102, 858, 1303
SspI AATATT 2 cut(s) 130, 494
SspMI CTAG 6 cut(s) 27, 312, 372, 516, 912, 1256
StuI AGGCCT 1 cut(s) 1168
StyD4I CCNGG 6 cut(s) 478, 812, 1085, 1129, 1497, 1515
StyI CCWWGG 2 cut(s) 867, 1174
TaaI ACNGT 3 cut(s) 236, 875, 1438
TaiI ACGT 1 cut(s) 885
TaqII GACCGA 1 cut(s) 722
TasI AATT 7 cut(s) 166, 376, 393, 484, 624, 1268, 1356
TatI WGTACW 3 cut(s) 161, 228, 518
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 9 cut(s) 340, 356, 463, 658, 683, 817, 905, 1021, 1090
Tru1I TTAA 2 cut(s) 779, 1568
Tru9I TTAA 2 cut(s) 779, 1568
TscAI CASTG 3 cut(s) 880, 1378, 1626
TseFI GTSAC 1 cut(s) 1283
TseI GCWGC 2 cut(s) 361, 1077
Tsp45I GTSAC 1 cut(s) 1283
TspDTI ATGAA 5 cut(s) 297, 353, 897, 1038, 1526
TspGWI ACGGA 1 cut(s) 231
TspRI CASTG 3 cut(s) 880, 1378, 1626
Tth111I GACNNNGTC 1 cut(s) 875
Van91I CCANNNNNTGG 1 cut(s) 1271
VpaK11BI GGWCC 4 cut(s) 446, 638, 769, 1133
XagI CCTNNNNNAGG 1 cut(s) 1173
XapI RAATTY 3 cut(s) 393, 484, 1268
XcmI CCANNNNNNNNNTGG 1 cut(s) 1370
XspI CTAG 6 cut(s) 27, 312, 372, 516, 912, 1256
ZrmI AGTACT 2 cut(s) 163, 520
Zsp2I ATGCAT 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.