Rroxscaffold_1G00060850

cationic amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0019265
Physical Location & Seq
Reverse (-)
83071033 .. 83071476
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060850.1

Sequence Viewer

Length: 444 bp
ATGGGGATGAGGGGAACGGAACCGAGCGGCGGCGGCGAGGGGCTGAAGAGGCGGGGGTGTTCCTGCGGAAAGAACGATTTTCTCCCGGAGGAGTCGTTCGAGAGCTGGAGCAATTACGCCAATGCGCTCCGGTCGACTCCGGCAAGGTTCAAGGACCGGTTCTTCACGCGGTCCGCCGACAGCACCGAGCTAGTGGAGGTGAAGGCGCGGAGCCAGCACGAGATGAAGAAGACGCTCAACTGGTGGGACCTCATCTGGTTCGGGCTCGGAGCCGTCATCGGCGCCGGAATCTTCGTCCTCACTGGACTCGAGGCCAATAAACACGCCGGACCCGCCGTCGTCATGTCCTACGCCGTCTCCGGCCTCTCCGCCTTGCTCTCTGTTTTCTGTTACACAGAGTTCGCCGTCGAGATTCCCGTTGCAGGTAACACTAATAAGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2 AA_permease_C

Protein Analysis

147

Amino Acids

16.04

Weight (kDa)

7.73

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease_2 PF13520 79 - 142 3.9e-06 Amino acid permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019265)

Species Orthologous Gene IDs
malus_domestica MD10G1226700.v1.1
pyrus_communis pycom16g10380
rosa_multiflora Rmu_co7960940.1_g000001 Rmu_sc0030939.1_g000001
rosa_roxburghii Rroxscaffold_1G00060850
rosa_samantha Rh5BG121300 Rh5CG131500 Rh5DG120100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 413
AccB1I GGYRCC 1 cut(s) 281
AccBSI CCGCTC 1 cut(s) 27
AccI GTMKAC 1 cut(s) 134
AccII CGCG 2 cut(s) 169, 208
AcuI CTGAAG 1 cut(s) 65
AcyI GRCGYC 1 cut(s) 282
AfiI CCNNNNNNNGG 2 cut(s) 29, 422
AgeI ACCGGT 1 cut(s) 156
AgsI TTSAA 1 cut(s) 151
AhdI GACNNNNNGTC 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 80, 112
AluBI AGCT 2 cut(s) 105, 190
AluI AGCT 2 cut(s) 105, 190
Alw26I GTCTC 1 cut(s) 361
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 2 cut(s) 312, 361
AsiGI ACCGGT 1 cut(s) 156
AspLEI GCGC 3 cut(s) 127, 208, 284
AspS9I GGNCC 4 cut(s) 154, 171, 247, 329
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 211
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 4 cut(s) 154, 171, 247, 329
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 236
BceAI ACGGC 4 cut(s) 257, 320, 338, 389
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 1 cut(s) 361
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 413
BisI GCNGC 3 cut(s) 28, 31, 34
BlsI GCNGC 3 cut(s) 29, 32, 35
Bme1390I CCNGG 1 cut(s) 86
Bme18I GGWCC 4 cut(s) 154, 171, 247, 329
BmeRI GACNNNNNGTC 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 4 cut(s) 154, 171, 247, 329
BmiI GGNNCC 6 cut(s) 21, 212, 248, 271, 283, 331
BmrFI CCNGG 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 236
BpmI CTGGAG 1 cut(s) 127
BpuMI CCSGG 1 cut(s) 86
BsaHI GRCGYC 1 cut(s) 282
BsaWI WCCGGW 2 cut(s) 129, 156
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 341, 371
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 422
Bse118I RCCGGY 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 245, 307
BseGI GGATG 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 29, 422
BseNI ACTGG 2 cut(s) 245, 307
BseRI GAGGAG 1 cut(s) 104
Bsh1236I CGCG 2 cut(s) 169, 208
Bsh1285I CGRYCG 1 cut(s) 134
BshFI GGCC 2 cut(s) 314, 363
BshNI GGYRCC 1 cut(s) 281
BshTI ACCGGT 1 cut(s) 156
BsiEI CGRYCG 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 7 cut(s) 86, 130, 140, 157, 285, 327, 360
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 2 cut(s) 29, 422
BsmAI GTCTC 1 cut(s) 361
BsmBI CGTCTC 1 cut(s) 361
BsmFI GGGAC 1 cut(s) 260
BsnI GGCC 2 cut(s) 314, 363
BsoBI CYCGRG 1 cut(s) 308
Bsp1286I GDGCHC 1 cut(s) 267
BspANI GGCC 2 cut(s) 314, 363
BspFNI CGCG 2 cut(s) 169, 208
BspLI GGNNCC 6 cut(s) 21, 212, 248, 271, 283, 331
BspMI ACCTGC 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 281
BsrBI CCGCTC 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 156
BsrI ACTGG 2 cut(s) 245, 307
BssAI RCCGGY 1 cut(s) 156
BssNI GRCGYC 1 cut(s) 282
BssSI CACGAG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 218
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 1 cut(s) 282
BstC8I GCNNGC 1 cut(s) 215
BstF5I GGATG 1 cut(s) 12
BstFNI CGCG 2 cut(s) 169, 208
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 3 cut(s) 127, 208, 284
BstMAI GTCTC 1 cut(s) 361
BstMCI CGRYCG 1 cut(s) 134
BstMWI GCNNNNNNNGC 4 cut(s) 33, 49, 214, 332
BstSCI CCNGG 1 cut(s) 84
BstUI CGCG 2 cut(s) 169, 208
BstV2I GAAGAC 1 cut(s) 236
BsuRI GGCC 2 cut(s) 314, 363
BtsCI GGATG 1 cut(s) 12
BtsIMutI CAGTG 1 cut(s) 300
BveI ACCTGC 1 cut(s) 413
Cac8I GCNNGC 1 cut(s) 215
CfoI GCGC 3 cut(s) 127, 208, 284
Cfr10I RCCGGY 1 cut(s) 156
Cfr13I GGNCC 4 cut(s) 154, 171, 247, 329
CpoI CGGWCCG 1 cut(s) 171
CseI GACGC 1 cut(s) 241
CspAI ACCGGT 1 cut(s) 156
CspI CGGWCCG 1 cut(s) 171
CviAII CATG 1 cut(s) 343
CviJI RGCY 8 cut(s) 43, 105, 190, 213, 265, 272, 314, 363
CviKI_1 RGCY 8 cut(s) 43, 105, 190, 213, 265, 272, 314, 363
DinI GGCGCC 1 cut(s) 283
DriI GACNNNNNGTC 1 cut(s) 335
Eam1104I CTCTTC 1 cut(s) 41
Eam1105I GACNNNNNGTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 2 cut(s) 163, 358
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 4 cut(s) 154, 171, 247, 329
Eco57I CTGAAG 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 247
EcoT38I GRGCYC 1 cut(s) 267
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
Esp3I CGTCTC 1 cut(s) 361
FaeI CATG 1 cut(s) 346
FaiI YATR 1 cut(s) 344
FaqI GGGAC 1 cut(s) 260
FatI CATG 1 cut(s) 342
FauI CCCGC 2 cut(s) 45, 340
FblI GTMKAC 1 cut(s) 134
Fnu4HI GCNGC 3 cut(s) 28, 31, 34
FokI GGATG 1 cut(s) 19
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 3 cut(s) 28, 31, 34
FspBI CTAG 1 cut(s) 191
GlaI GCGC 3 cut(s) 126, 207, 283
GluI GCNGC 3 cut(s) 28, 31, 34
GsuI CTGGAG 1 cut(s) 127
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 2 cut(s) 314, 363
HapII CCGG 7 cut(s) 86, 130, 140, 157, 285, 327, 360
HgaI GACGC 1 cut(s) 241
HhaI GCGC 3 cut(s) 127, 208, 284
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 1 cut(s) 346
Hin6I GCGC 3 cut(s) 125, 206, 282
HinP1I GCGC 3 cut(s) 125, 206, 282
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HinfI GANTC 5 cut(s) 92, 136, 288, 306, 412
HpaII CCGG 7 cut(s) 86, 130, 140, 157, 285, 327, 360
HphI GGTGA 1 cut(s) 211
Hpy166II GTNNAC 1 cut(s) 135
Hpy188I TCNGA 1 cut(s) 269
Hpy188III TCNNGA 2 cut(s) 100, 409
Hpy8I GTNNAC 1 cut(s) 135
Hpy99I CGWCG 2 cut(s) 341, 410
HpyAV CCTTC 1 cut(s) 196
HpyCH4V TGCA 1 cut(s) 422
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 49, 214, 332
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 1 cut(s) 346
HspAI GCGC 3 cut(s) 125, 206, 282
KasI GGCGCC 1 cut(s) 281
LmnI GCTCC 4 cut(s) 108, 132, 210, 269
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 2 cut(s) 389, 425
MbiI CCGCTC 1 cut(s) 27
MboII GAAGA 5 cut(s) 58, 154, 238, 241, 283
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 1 cut(s) 112
Mly113I GGCGCC 1 cut(s) 282
MlyI GAGTC 3 cut(s) 101, 130, 300
MnlI CCTC 9 cut(s) 3, 31, 42, 82, 190, 260, 304, 308, 374
MseI TTAA 1 cut(s) 442
MspI CCGG 7 cut(s) 86, 130, 140, 157, 285, 327, 360
MspR9I CCNGG 1 cut(s) 86
MvnI CGCG 2 cut(s) 169, 208
MwoI GCNNNNNNNGC 4 cut(s) 33, 49, 214, 332
NarI GGCGCC 1 cut(s) 282
NciI CCSGG 1 cut(s) 86
NlaIII CATG 1 cut(s) 346
NlaIV GGNNCC 6 cut(s) 21, 212, 248, 271, 283, 331
PaeR7I CTCGAG 1 cut(s) 308
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 2 cut(s) 288, 412
PfoI TCCNGGA 1 cut(s) 84
PinAI ACCGGT 1 cut(s) 156
PkrI GCNGC 3 cut(s) 29, 32, 35
PleI GAGTC 3 cut(s) 100, 130, 300
PluTI GGCGCC 1 cut(s) 285
PpsI GAGTC 3 cut(s) 100, 130, 300
PpuMI RGGWCCY 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 247
PspN4I GGNNCC 6 cut(s) 21, 212, 248, 271, 283, 331
PspPI GGNCC 4 cut(s) 154, 171, 247, 329
PspPPI RGGWCCY 1 cut(s) 247
PspXI VCTCGAGB 1 cut(s) 308
Rsr2I CGGWCCG 1 cut(s) 171
RsrII CGGWCCG 1 cut(s) 171
SalI GTCGAC 1 cut(s) 133
SaqAI TTAA 1 cut(s) 442
SatI GCNGC 3 cut(s) 28, 31, 34
Sau96I GGNCC 4 cut(s) 154, 171, 247, 329
SchI GAGTC 3 cut(s) 101, 130, 300
ScrFI CCNGG 1 cut(s) 86
SduI GDGCHC 1 cut(s) 267
SetI ASST 6 cut(s) 107, 149, 192, 201, 252, 427
SfoI GGCGCC 1 cut(s) 283
Sfr274I CTCGAG 1 cut(s) 308
SinI GGWCC 4 cut(s) 154, 171, 247, 329
SlaI CTCGAG 1 cut(s) 308
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 1 cut(s) 112
SspDI GGCGCC 1 cut(s) 281
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 1 cut(s) 84
TaqI TCGA 4 cut(s) 99, 134, 309, 408
TasI AATT 1 cut(s) 112
TauI GCSGC 3 cut(s) 30, 33, 36
TfiI GAWTC 2 cut(s) 288, 412
Tru1I TTAA 1 cut(s) 442
Tru9I TTAA 1 cut(s) 442
TscAI CASTG 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 239
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 1 cut(s) 307
VpaK11BI GGWCC 4 cut(s) 154, 171, 247, 329
XhoI CTCGAG 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 134
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.