Rh5DG120100

cationic amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0019265
Physical Location & Seq
Forward (+)
10866158 .. 10870283
4126 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG120100.1

Sequence Viewer

Length: 882 bp
ATGGGGATGAGCGGAACGGAACCGAGCGGCGGAGGCGAGGGACTGAAGAGGCGGTGGTGTTCGTGCGGAAAGAACGATTTTCTCCCGGAGGAGTCGTTCGAGAGCTGGGGCAATTACGCCAATGCGCTCCGGTCGAATCCGGCGAGGTTCAAGGACAGGTTCTTCACGCGGTCCGCCTACAGCACCGAGCTGGTGGAGGTGAAGGCGCGGAGCCAGCACGAGATGAAGAAGACGCTTGACTGGTGGGACCTCATCTGGTTCGGGCTCGGAGCTGTCATCGGCGCTGGAATCTTCGTCCTCACTGGATTCGAGGCCAAGAGACACGCCGGACCCGCCGTCGTCATGTCCTACGTCGTCTCCGGCGTCTCCGCCTTGCTCTCTGTTTTCTGTTACACAGAGTTCGCCGTCGAGATTCCTGTTGCAGGCGGCTCATCTGCCTACCTAAGAGTGGAGCTTGGGGACTTCGTGGCCTACATTGCAGCAGGCAACATCCTTCTCGAATATGTCATAGGCAGGGCGGCCGTTGCGCGTTCGTGGACCTCCTACTTCACCTCCCTATGCAACCAGGACGATTCCAATGCTTTCCGAATCACTGTCCACGGTCTCCTAGCAGGTCTCACCAAAGCAGATACTGACAACTACAAGGAGTTTGCCCCAAATGGTGCTCGTGGCATCTTCCAAGCCTCGGCAGTTCTCTTCTTTGCATACGTTGGATTTGACGCCGTTTCAACCATGGCCGAAGAGACCAAGAATCCTGCTCGAGACATCCCAATAGGGCTGGTTGGGTCCATGTTGATCACCACCACTCTTTATTGCTTGCTAGCCGTGACACTCTGTCTTATGGTACCATATCAGGTAATCGATGAGAACGCTCCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2 AA_permease_C

Protein Analysis

293

Amino Acids

31.93

Weight (kDa)

5.33

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease_2 PF13520 79 - 190 4.6e-11 Amino acid permease
AA_permease PF00324 84 - 182 2e-07 Amino acid permease
AA_permease_2 PF13520 209 - 291 1.5e-10 Amino acid permease
AA_permease PF00324 211 - 285 2.3e-12 Amino acid permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019265)

Species Orthologous Gene IDs
malus_domestica MD10G1226700.v1.1
pyrus_communis pycom16g10380
rosa_multiflora Rmu_co7960940.1_g000001 Rmu_sc0030939.1_g000001
rosa_roxburghii Rroxscaffold_1G00060850
rosa_samantha Rh5BG121300 Rh5CG131500 Rh5DG120100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 602
Acc65I GGTACC 1 cut(s) 844
AccB1I GGYRCC 1 cut(s) 844
AccBSI CCGCTC 2 cut(s) 12, 27
AccII CGCG 3 cut(s) 169, 208, 529
AcoI YGGCCR 2 cut(s) 519, 735
AcuI CTGAAG 1 cut(s) 65
AcyI GRCGYC 2 cut(s) 363, 720
AfaI GTAC 1 cut(s) 846
AfiI CCNNNNNNNGG 4 cut(s) 29, 422, 448, 685
AgsI TTSAA 2 cut(s) 151, 729
AhdI GACNNNNNGTC 2 cut(s) 335, 834
AjnI CCWGG 1 cut(s) 564
AloI GAACNNNNNNTCC 2 cut(s) 80, 112
AluBI AGCT 4 cut(s) 105, 190, 272, 454
AluI AGCT 4 cut(s) 105, 190, 272, 454
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 7 cut(s) 313, 361, 370, 608, 620, 737, 756
AlwNI CAGNNNCTG 1 cut(s) 632
Ama87I CYCGRG 1 cut(s) 759
AoxI GGCC 4 cut(s) 312, 468, 519, 735
ApeKI GCWGC 1 cut(s) 479
Asp718I GGTACC 1 cut(s) 844
AspLEI GCGC 4 cut(s) 127, 208, 284, 529
AspS9I GGNCC 5 cut(s) 171, 247, 329, 537, 786
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 4 cut(s) 211, 541, 610, 790
AsuNHI GCTAGC 1 cut(s) 820
AvaI CYCGRG 1 cut(s) 759
AvaII GGWCC 5 cut(s) 171, 247, 329, 537, 786
BanI GGYRCC 1 cut(s) 844
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 2 cut(s) 218, 666
BbsI GAAGAC 1 cut(s) 236
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 1 cut(s) 491
BceAI ACGGC 5 cut(s) 320, 389, 506, 707, 809
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 1 cut(s) 566
BclI TGATCA 1 cut(s) 795
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 7 cut(s) 313, 361, 370, 608, 620, 737, 756
BfaI CTAG 2 cut(s) 608, 821
BfmI CTRYAG 1 cut(s) 178
BfoI RGCGCY 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 602
BisI GCNGC 4 cut(s) 28, 427, 480, 519
BlsI GCNGC 4 cut(s) 29, 428, 481, 520
Bme1390I CCNGG 2 cut(s) 86, 566
Bme18I GGWCC 5 cut(s) 171, 247, 329, 537, 786
BmeRI GACNNNNNGTC 2 cut(s) 335, 834
BmeT110I CYCGRG 1 cut(s) 759
BmgT120I GGNCC 5 cut(s) 171, 247, 329, 537, 786
BmiI GGNNCC 6 cut(s) 21, 212, 248, 331, 787, 846
BmrFI CCNGG 2 cut(s) 86, 566
BmsI GCATC 1 cut(s) 681
BmtI GCTAGC 1 cut(s) 824
BpiI GAAGAC 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 86
Bsa29I ATCGAT 1 cut(s) 861
BsaHI GRCGYC 2 cut(s) 363, 720
BsaI GGTCTC 3 cut(s) 608, 620, 737
BsaJI CCNNGG 3 cut(s) 598, 684, 732
BsaWI WCCGGW 1 cut(s) 129
BsaXI ACNNNNNCTCC 6 cut(s) 80, 110, 341, 371, 536, 566
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 422, 448, 685
Bse1I ACTGG 2 cut(s) 245, 307
Bse3DI GCAATG 1 cut(s) 474
BseBI CCWGG 1 cut(s) 566
BseCI ATCGAT 1 cut(s) 861
BseDI CCNNGG 3 cut(s) 598, 684, 732
BseGI GGATG 3 cut(s) 12, 489, 765
BseLI CCNNNNNNNGG 4 cut(s) 29, 422, 448, 685
BseMI GCAATG 1 cut(s) 474
BseNI ACTGG 2 cut(s) 245, 307
BseRI GAGGAG 1 cut(s) 104
BseX3I CGGCCG 1 cut(s) 519
BseXI GCAGC 1 cut(s) 491
BseYI CCCAGC 1 cut(s) 105
Bsh1236I CGCG 3 cut(s) 169, 208, 529
Bsh1285I CGRYCG 2 cut(s) 134, 522
BshFI GGCC 4 cut(s) 314, 470, 521, 737
BshNI GGYRCC 1 cut(s) 844
BshVI ATCGAT 1 cut(s) 861
BsiEI CGRYCG 2 cut(s) 134, 522
BsiHKAI GWGCWC 1 cut(s) 667
BsiHKCI CYCGRG 1 cut(s) 759
BsiSI CCGG 5 cut(s) 86, 130, 140, 327, 360
BslFI GGGAC 3 cut(s) 54, 260, 473
BslI CCNNNNNNNGG 4 cut(s) 29, 422, 448, 685
BsmAI GTCTC 7 cut(s) 313, 361, 370, 608, 620, 737, 756
BsmBI CGTCTC 2 cut(s) 361, 370
BsmFI GGGAC 3 cut(s) 54, 260, 473
BsnI GGCC 4 cut(s) 314, 470, 521, 737
Bso31I GGTCTC 3 cut(s) 608, 620, 737
BsoBI CYCGRG 1 cut(s) 759
Bsp1286I GDGCHC 2 cut(s) 267, 667
Bsp143I GATC 1 cut(s) 795
Bsp19I CCATGG 1 cut(s) 732
BspANI GGCC 4 cut(s) 314, 470, 521, 737
BspDI ATCGAT 1 cut(s) 861
BspFNI CGCG 3 cut(s) 169, 208, 529
BspLI GGNNCC 6 cut(s) 21, 212, 248, 331, 787, 846
BspMI ACCTGC 1 cut(s) 602
BspOI GCTAGC 1 cut(s) 824
BspT107I GGYRCC 1 cut(s) 844
BspTNI GGTCTC 3 cut(s) 608, 620, 737
BsrBI CCGCTC 2 cut(s) 12, 27
BsrDI GCAATG 1 cut(s) 474
BsrI ACTGG 2 cut(s) 245, 307
BssECI CCNNGG 3 cut(s) 598, 684, 732
BssMI GATC 1 cut(s) 795
BssNI GRCGYC 2 cut(s) 363, 720
BssSI CACGAG 2 cut(s) 218, 666
BssT1I CCWWGG 1 cut(s) 732
Bst2BI CACGAG 2 cut(s) 218, 666
Bst2UI CCWGG 1 cut(s) 566
Bst4CI ACNGT 2 cut(s) 595, 602
Bst6I CTCTTC 3 cut(s) 41, 701, 735
BstACI GRCGYC 2 cut(s) 363, 720
BstC8I GCNNGC 5 cut(s) 215, 424, 484, 818, 822
BstDEI CTNAG 1 cut(s) 443
BstDSI CCRYGG 2 cut(s) 598, 732
BstENI CCTNNNNNAGG 1 cut(s) 420
BstF5I GGATG 3 cut(s) 12, 489, 765
BstFNI CGCG 3 cut(s) 169, 208, 529
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 4 cut(s) 127, 208, 284, 529
BstKTI GATC 1 cut(s) 798
BstMAI GTCTC 7 cut(s) 313, 361, 370, 608, 620, 737, 756
BstMBI GATC 1 cut(s) 795
BstMCI CGRYCG 2 cut(s) 134, 522
BstMWI GCNNNNNNNGC 5 cut(s) 33, 214, 332, 476, 524
BstNI CCWGG 1 cut(s) 566
BstSCI CCNGG 2 cut(s) 84, 564
BstSFI CTRYAG 1 cut(s) 178
BstUI CGCG 3 cut(s) 169, 208, 529
BstV1I GCAGC 1 cut(s) 491
BstV2I GAAGAC 1 cut(s) 236
BstZI CGGCCG 1 cut(s) 519
Bsu15I ATCGAT 1 cut(s) 861
BsuRI GGCC 4 cut(s) 314, 470, 521, 737
BsuTUI ATCGAT 1 cut(s) 861
BtgI CCRYGG 2 cut(s) 598, 732
BtsCI GGATG 3 cut(s) 12, 489, 765
BtsIMutI CAGTG 2 cut(s) 300, 591
BveI ACCTGC 1 cut(s) 602
Cac8I GCNNGC 5 cut(s) 215, 424, 484, 818, 822
CaiI CAGNNNCTG 1 cut(s) 632
CfoI GCGC 4 cut(s) 127, 208, 284, 529
Cfr13I GGNCC 5 cut(s) 171, 247, 329, 537, 786
ClaI ATCGAT 1 cut(s) 861
CpoI CGGWCCG 1 cut(s) 171
CseI GACGC 3 cut(s) 241, 352, 728
Csp6I GTAC 1 cut(s) 845
CspI CGGWCCG 1 cut(s) 171
CviAII CATG 3 cut(s) 343, 733, 790
CviQI GTAC 1 cut(s) 845
DdeI CTNAG 1 cut(s) 443
DpnI GATC 1 cut(s) 797
DpnII GATC 1 cut(s) 795
DriI GACNNNNNGTC 2 cut(s) 335, 834
EaeI YGGCCR 2 cut(s) 519, 735
EagI CGGCCG 1 cut(s) 519
Eam1104I CTCTTC 3 cut(s) 41, 701, 735
Eam1105I GACNNNNNGTC 2 cut(s) 335, 834
EarI CTCTTC 3 cut(s) 41, 701, 735
EciI GGCGGA 3 cut(s) 45, 163, 358
EclXI CGGCCG 1 cut(s) 519
Eco130I CCWWGG 1 cut(s) 732
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 3 cut(s) 608, 620, 737
Eco47I GGWCC 5 cut(s) 171, 247, 329, 537, 786
Eco52I CGGCCG 1 cut(s) 519
Eco57I CTGAAG 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 759
EcoNI CCTNNNNNAGG 1 cut(s) 420
EcoO109I RGGNCCY 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 564
EcoT14I CCWWGG 1 cut(s) 732
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 732
Esp3I CGTCTC 2 cut(s) 361, 370
FaeI CATG 3 cut(s) 346, 736, 793
FaiI YATR 9 cut(s) 344, 504, 509, 559, 706, 734, 791, 842, 850
FaqI GGGAC 3 cut(s) 54, 260, 473
FatI CATG 3 cut(s) 342, 732, 789
FauI CCCGC 1 cut(s) 340
FbaI TGATCA 1 cut(s) 795
Fnu4HI GCNGC 4 cut(s) 28, 427, 480, 519
FokI GGATG 3 cut(s) 19, 476, 752
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 4 cut(s) 28, 427, 480, 519
FspBI CTAG 2 cut(s) 608, 821
GlaI GCGC 4 cut(s) 126, 207, 283, 528
GluI GCNGC 4 cut(s) 28, 427, 480, 519
GsaI CCCAGC 1 cut(s) 109
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 4 cut(s) 314, 470, 521, 737
HapII CCGG 5 cut(s) 86, 130, 140, 327, 360
HgaI GACGC 3 cut(s) 241, 352, 728
HhaI GCGC 4 cut(s) 127, 208, 284, 529
Hin1I GRCGYC 2 cut(s) 363, 720
Hin1II CATG 3 cut(s) 346, 736, 793
Hin6I GCGC 4 cut(s) 125, 206, 282, 527
HinP1I GCGC 4 cut(s) 125, 206, 282, 527
HinfI GANTC 8 cut(s) 92, 136, 288, 306, 412, 572, 588, 751
HpaII CCGG 5 cut(s) 86, 130, 140, 327, 360
HphI GGTGA 4 cut(s) 211, 541, 610, 790
Hpy166II GTNNAC 2 cut(s) 537, 598
Hpy188I TCNGA 2 cut(s) 269, 587
Hpy188III TCNNGA 4 cut(s) 100, 409, 497, 761
Hpy8I GTNNAC 2 cut(s) 537, 598
Hpy99I CGWCG 3 cut(s) 341, 356, 410
HpyAV CCTTC 2 cut(s) 196, 503
HpyCH4III ACNGT 2 cut(s) 595, 602
HpyCH4IV ACGT 2 cut(s) 351, 708
HpyCH4V TGCA 4 cut(s) 422, 479, 561, 704
HpyF10VI GCNNNNNNNGC 5 cut(s) 33, 214, 332, 476, 524
HpyF3I CTNAG 1 cut(s) 443
HpySE526I ACGT 2 cut(s) 351, 708
Hsp92I GRCGYC 2 cut(s) 363, 720
Hsp92II CATG 3 cut(s) 346, 736, 793
HspAI GCGC 4 cut(s) 125, 206, 282, 527
KpnI GGTACC 1 cut(s) 848
Ksp22I TGATCA 1 cut(s) 795
Kzo9I GATC 1 cut(s) 795
LmnI GCTCC 5 cut(s) 132, 210, 269, 451, 877
Lsp1109I GCAGC 1 cut(s) 491
LweI GCATC 1 cut(s) 681
MaeI CTAG 2 cut(s) 608, 821
MaeII ACGT 2 cut(s) 351, 708
MaeIII GTNAC 2 cut(s) 389, 826
MalI GATC 1 cut(s) 797
MbiI CCGCTC 2 cut(s) 12, 27
MboI GATC 1 cut(s) 795
MboII GAAGA 8 cut(s) 58, 154, 238, 241, 283, 667, 688, 752
MhlI GDGCHC 2 cut(s) 267, 667
MluCI AATT 1 cut(s) 112
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 691
MseI TTAA 1 cut(s) 880
MspI CCGG 5 cut(s) 86, 130, 140, 327, 360
MspR9I CCNGG 2 cut(s) 86, 566
MvaI CCWGG 1 cut(s) 566
MvnI CGCG 3 cut(s) 169, 208, 529
MwoI GCNNNNNNNGC 5 cut(s) 33, 214, 332, 476, 524
NciI CCSGG 1 cut(s) 86
NcoI CCATGG 1 cut(s) 732
NdeII GATC 1 cut(s) 795
NheI GCTAGC 1 cut(s) 820
NlaIII CATG 3 cut(s) 346, 736, 793
NlaIV GGNNCC 6 cut(s) 21, 212, 248, 331, 787, 846
NmeAIII GCCGAG 1 cut(s) 665
NmuCI GTSAC 1 cut(s) 826
PaeR7I CTCGAG 1 cut(s) 759
PcsI WCGNNNNNNNCGW 2 cut(s) 140, 360
PfeI GAWTC 7 cut(s) 136, 288, 306, 412, 572, 588, 751
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 4 cut(s) 29, 428, 481, 520
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
PpuMI RGGWCCY 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 564
PspFI CCCAGC 1 cut(s) 105
PspGI CCWGG 1 cut(s) 564
PspN4I GGNNCC 6 cut(s) 21, 212, 248, 331, 787, 846
PspPI GGNCC 5 cut(s) 171, 247, 329, 537, 786
PspPPI RGGWCCY 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 632
RsaI GTAC 1 cut(s) 846
RsaNI GTAC 1 cut(s) 845
Rsr2I CGGWCCG 1 cut(s) 171
RsrII CGGWCCG 1 cut(s) 171
SaqAI TTAA 1 cut(s) 880
SatI GCNGC 4 cut(s) 28, 427, 480, 519
Sau3AI GATC 1 cut(s) 795
Sau96I GGNCC 5 cut(s) 171, 247, 329, 537, 786
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 2 cut(s) 86, 566
SduI GDGCHC 2 cut(s) 267, 667
SfaNI GCATC 1 cut(s) 681
SfcI CTRYAG 1 cut(s) 178
Sfr274I CTCGAG 1 cut(s) 759
SinI GGWCC 5 cut(s) 171, 247, 329, 537, 786
SlaI CTCGAG 1 cut(s) 759
SmlI CTYRAG 1 cut(s) 759
SmoI CTYRAG 1 cut(s) 759
Sse9I AATT 1 cut(s) 112
SspMI CTAG 2 cut(s) 608, 821
StyD4I CCNGG 2 cut(s) 84, 564
StyI CCWWGG 1 cut(s) 732
TaaI ACNGT 2 cut(s) 595, 602
TaiI ACGT 2 cut(s) 354, 711
TaqI TCGA 7 cut(s) 99, 134, 309, 408, 498, 760, 861
TasI AATT 1 cut(s) 112
TauI GCSGC 3 cut(s) 30, 429, 521
TfiI GAWTC 7 cut(s) 136, 288, 306, 412, 572, 588, 751
Tru1I TTAA 1 cut(s) 880
Tru9I TTAA 1 cut(s) 880
TscAI CASTG 2 cut(s) 307, 598
TseFI GTSAC 1 cut(s) 826
TseI GCWGC 1 cut(s) 479
Tsp45I GTSAC 1 cut(s) 826
TspDTI ATGAA 1 cut(s) 239
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 2 cut(s) 307, 598
VpaK11BI GGWCC 5 cut(s) 171, 247, 329, 537, 786
XagI CCTNNNNNAGG 1 cut(s) 420
XhoI CTCGAG 1 cut(s) 759
XspI CTAG 2 cut(s) 608, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.