Rh5BG121300

cationic amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0019265
Physical Location & Seq
Forward (+)
11473227 .. 11477184
3958 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG121300.1

Sequence Viewer

Length: 432 bp
ATGGGGATGAGCGGAACAGAACCGAGCGGCGGCGGCGAGGGACTGAAGAGGCGGGGGTGTTCGTGCGGAAAGAACGATTTTCTCCCGGAGGAGTCGTTCGAGAGCTGGGGCAATTACGCCAATGCGCTCCGGTCGACTCCTGCGAGGTTCAAGGACAGGTTCTTCACGCGGTCCTCCGACAGCACCGAGCTGGTGGAGGTGAAGGCGCAGAGCCAGCACGAGATGAAGAAGGCGCTTGACTGGTGGGACCTCATCTGGTTCGGGCTCGGAGCCGTCATCGGCGCCGGAATCTTCGTCCTCACTGGACTCGAGGCCAAGCAACACGCCGGACCCGCCGTCGTCATGTCCTACGTCGTCTCCGGCGTCTCCGCCTTGCTCTCTGTTTTCTGTTACACAGAGTTCGCCGTCGAGATTCCCGTTGCAGGAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2 AA_permease_C

Protein Analysis

143

Amino Acids

15.39

Weight (kDa)

5.37

Isoelectric Point (pI)

55.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease_2 PF13520 79 - 143 1.2e-06 Amino acid permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019265)

Species Orthologous Gene IDs
malus_domestica MD10G1226700.v1.1
pyrus_communis pycom16g10380
rosa_multiflora Rmu_co7960940.1_g000001 Rmu_sc0030939.1_g000001
rosa_roxburghii Rroxscaffold_1G00060850
rosa_samantha Rh5BG121300 Rh5CG131500 Rh5DG120100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 281
AccBSI CCGCTC 2 cut(s) 12, 27
AccI GTMKAC 1 cut(s) 134
AccII CGCG 1 cut(s) 169
AciI CCGC 9 cut(s) 12, 27, 30, 33, 52, 66, 169, 333, 369
AcuI CTGAAG 1 cut(s) 65
AcyI GRCGYC 2 cut(s) 282, 363
AfiI CCNNNNNNNGG 2 cut(s) 29, 422
AgsI TTSAA 1 cut(s) 151
AhdI GACNNNNNGTC 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 80, 112
AluBI AGCT 2 cut(s) 105, 190
AluI AGCT 2 cut(s) 105, 190
Alw26I GTCTC 2 cut(s) 361, 370
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 1 cut(s) 312
AspLEI GCGC 4 cut(s) 127, 208, 235, 284
AspS9I GGNCC 3 cut(s) 171, 247, 329
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 211
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 3 cut(s) 171, 247, 329
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 218
BceAI ACGGC 3 cut(s) 257, 320, 389
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 2 cut(s) 361, 370
BfoI RGCGCY 2 cut(s) 236, 285
BisI GCNGC 3 cut(s) 28, 31, 34
BlsI GCNGC 3 cut(s) 29, 32, 35
Bme1390I CCNGG 1 cut(s) 86
Bme18I GGWCC 3 cut(s) 171, 247, 329
BmeRI GACNNNNNGTC 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 3 cut(s) 171, 247, 329
BmiI GGNNCC 4 cut(s) 248, 271, 283, 331
BmrFI CCNGG 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 86
BsaHI GRCGYC 2 cut(s) 282, 363
BsaWI WCCGGW 1 cut(s) 129
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 341, 371
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 422
Bse1I ACTGG 2 cut(s) 245, 307
BseGI GGATG 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 29, 422
BseNI ACTGG 2 cut(s) 245, 307
BseRI GAGGAG 1 cut(s) 104
BseYI CCCAGC 1 cut(s) 105
Bsh1236I CGCG 1 cut(s) 169
Bsh1285I CGRYCG 1 cut(s) 134
BshFI GGCC 1 cut(s) 314
BshNI GGYRCC 1 cut(s) 281
BsiEI CGRYCG 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 5 cut(s) 86, 130, 285, 327, 360
BslFI GGGAC 2 cut(s) 54, 260
BslI CCNNNNNNNGG 2 cut(s) 29, 422
BsmAI GTCTC 2 cut(s) 361, 370
BsmBI CGTCTC 2 cut(s) 361, 370
BsmFI GGGAC 2 cut(s) 54, 260
BsnI GGCC 1 cut(s) 314
BsoBI CYCGRG 1 cut(s) 308
Bsp1286I GDGCHC 1 cut(s) 267
BspACI CCGC 9 cut(s) 12, 27, 30, 33, 52, 66, 169, 333, 369
BspANI GGCC 1 cut(s) 314
BspFNI CGCG 1 cut(s) 169
BspLI GGNNCC 4 cut(s) 248, 271, 283, 331
BspT107I GGYRCC 1 cut(s) 281
BsrBI CCGCTC 2 cut(s) 12, 27
BsrI ACTGG 2 cut(s) 245, 307
BssNI GRCGYC 2 cut(s) 282, 363
BssSI CACGAG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 218
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 2 cut(s) 282, 363
BstC8I GCNNGC 1 cut(s) 215
BstF5I GGATG 1 cut(s) 12
BstFNI CGCG 1 cut(s) 169
BstH2I RGCGCY 2 cut(s) 236, 285
BstHHI GCGC 4 cut(s) 127, 208, 235, 284
BstMAI GTCTC 2 cut(s) 361, 370
BstMCI CGRYCG 1 cut(s) 134
BstMWI GCNNNNNNNGC 3 cut(s) 33, 214, 332
BstSCI CCNGG 1 cut(s) 84
BstUI CGCG 1 cut(s) 169
BsuRI GGCC 1 cut(s) 314
BtsCI GGATG 1 cut(s) 12
BtsIMutI CAGTG 1 cut(s) 300
Cac8I GCNNGC 1 cut(s) 215
CfoI GCGC 4 cut(s) 127, 208, 235, 284
Cfr13I GGNCC 3 cut(s) 171, 247, 329
CseI GACGC 1 cut(s) 352
CviAII CATG 1 cut(s) 343
CviJI RGCY 6 cut(s) 105, 190, 213, 265, 272, 314
CviKI_1 RGCY 6 cut(s) 105, 190, 213, 265, 272, 314
DinI GGCGCC 1 cut(s) 283
DriI GACNNNNNGTC 1 cut(s) 335
Eam1104I CTCTTC 1 cut(s) 41
Eam1105I GACNNNNNGTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 3 cut(s) 171, 247, 329
Eco57I CTGAAG 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 247
EcoT38I GRGCYC 1 cut(s) 267
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
Esp3I CGTCTC 2 cut(s) 361, 370
FaeI CATG 1 cut(s) 346
FaiI YATR 1 cut(s) 344
FaqI GGGAC 2 cut(s) 54, 260
FatI CATG 1 cut(s) 342
FauI CCCGC 2 cut(s) 45, 340
FblI GTMKAC 1 cut(s) 134
Fnu4HI GCNGC 3 cut(s) 28, 31, 34
FokI GGATG 1 cut(s) 19
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 3 cut(s) 28, 31, 34
GlaI GCGC 4 cut(s) 126, 207, 234, 283
GluI GCNGC 3 cut(s) 28, 31, 34
GsaI CCCAGC 1 cut(s) 109
HaeII RGCGCY 2 cut(s) 236, 285
HaeIII GGCC 1 cut(s) 314
HapII CCGG 5 cut(s) 86, 130, 285, 327, 360
HgaI GACGC 1 cut(s) 352
HhaI GCGC 4 cut(s) 127, 208, 235, 284
Hin1I GRCGYC 2 cut(s) 282, 363
Hin1II CATG 1 cut(s) 346
Hin6I GCGC 4 cut(s) 125, 206, 233, 282
HinP1I GCGC 4 cut(s) 125, 206, 233, 282
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HinfI GANTC 5 cut(s) 92, 136, 288, 306, 412
HpaII CCGG 5 cut(s) 86, 130, 285, 327, 360
HphI GGTGA 1 cut(s) 211
Hpy166II GTNNAC 1 cut(s) 135
Hpy188I TCNGA 2 cut(s) 178, 269
Hpy188III TCNNGA 2 cut(s) 100, 409
Hpy8I GTNNAC 1 cut(s) 135
Hpy99I CGWCG 3 cut(s) 341, 356, 410
HpyAV CCTTC 2 cut(s) 196, 223
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 1 cut(s) 422
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 214, 332
HpySE526I ACGT 1 cut(s) 351
Hsp92I GRCGYC 2 cut(s) 282, 363
Hsp92II CATG 1 cut(s) 346
HspAI GCGC 4 cut(s) 125, 206, 233, 282
KasI GGCGCC 1 cut(s) 281
LmnI GCTCC 2 cut(s) 132, 269
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 1 cut(s) 389
MbiI CCGCTC 2 cut(s) 12, 27
MboII GAAGA 4 cut(s) 58, 154, 238, 283
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 1 cut(s) 112
Mly113I GGCGCC 1 cut(s) 282
MlyI GAGTC 3 cut(s) 101, 130, 300
MmeI TCCRAC 1 cut(s) 201
MspI CCGG 5 cut(s) 86, 130, 285, 327, 360
MspR9I CCNGG 1 cut(s) 86
MvnI CGCG 1 cut(s) 169
MwoI GCNNNNNNNGC 3 cut(s) 33, 214, 332
NarI GGCGCC 1 cut(s) 282
NciI CCSGG 1 cut(s) 86
NlaIII CATG 1 cut(s) 346
NlaIV GGNNCC 4 cut(s) 248, 271, 283, 331
PaeR7I CTCGAG 1 cut(s) 308
PcsI WCGNNNNNNNCGW 3 cut(s) 140, 360, 414
PfeI GAWTC 2 cut(s) 288, 412
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 3 cut(s) 29, 32, 35
PleI GAGTC 3 cut(s) 100, 130, 300
PluTI GGCGCC 1 cut(s) 285
PpsI GAGTC 3 cut(s) 100, 130, 300
PpuMI RGGWCCY 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 247
PspFI CCCAGC 1 cut(s) 105
PspN4I GGNNCC 4 cut(s) 248, 271, 283, 331
PspPI GGNCC 3 cut(s) 171, 247, 329
PspPPI RGGWCCY 1 cut(s) 247
PspXI VCTCGAGB 1 cut(s) 308
SalI GTCGAC 1 cut(s) 133
SatI GCNGC 3 cut(s) 28, 31, 34
Sau96I GGNCC 3 cut(s) 171, 247, 329
SchI GAGTC 3 cut(s) 101, 130, 300
ScrFI CCNGG 1 cut(s) 86
SduI GDGCHC 1 cut(s) 267
SetI ASST 8 cut(s) 107, 149, 161, 192, 201, 252, 354, 430
SfoI GGCGCC 1 cut(s) 283
Sfr274I CTCGAG 1 cut(s) 308
SinI GGWCC 3 cut(s) 171, 247, 329
SlaI CTCGAG 1 cut(s) 308
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 1 cut(s) 112
SsiI CCGC 9 cut(s) 12, 27, 30, 33, 52, 66, 169, 333, 369
SspDI GGCGCC 1 cut(s) 281
StyD4I CCNGG 1 cut(s) 84
TaiI ACGT 1 cut(s) 354
TaqI TCGA 4 cut(s) 99, 134, 309, 408
TasI AATT 1 cut(s) 112
TauI GCSGC 3 cut(s) 30, 33, 36
TfiI GAWTC 2 cut(s) 288, 412
TscAI CASTG 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 239
TspRI CASTG 1 cut(s) 307
VpaK11BI GGWCC 3 cut(s) 171, 247, 329
XhoI CTCGAG 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.