Rroxscaffold_2G00082640

Glutaredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012964
Physical Location & Seq
Forward (+)
5424748 .. 5427079
2332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082640.1

Sequence Viewer

Length: 942 bp
ATGTCCTGTTCCCGTTTCAGGGGTGTTGATCGTCCCAAGTCAAGGTTTCAAGAAAATGGTGCGGCCTCGCCTAAGCCTCTGTGGCTTCAATTGGCTGATTATGAGAATTTCAACTCAAATGTTGGGATTCCACAATTTGATCCGGAGGTCCTTTCCGGATTTAGAAAGTCGTTCGAAGAGCTCGAGGAAGATGATCCATTTCATTTTAAAGATAGAGAAGAGCTCAATGAGGATGATTCTTTTCATTTCATGGATAGAGAAGAAGAGCTCAAGGAGGATGATTCTTTTCATTTCAAGGATAGAGAAGAAGAGCCCAAGGCGGATGATTCTTTTCGTTTTTCGGATGAAAAGGAGGTAAATAGCTTCATTTCGGATTACAAGTGTGAAGTGCCTTTTGGTAAGGCGAAGGATAGAGAGAAAGTGGTGTTGTACTTCACTAGTCTTAGAGGCGTGCGGAAGACTTATGAGGATTGTTGCCATGTGAGGGTGATTCTAAAAGGCATAGGTGTTCGGGTTGATGAGCGAGATGTTTCGATGCATTCGGGGTTCAAGGAGGAGTTGAAGGAGCTAGTGAAAGATGGGTGTAGTGGAGTTTCTTTGCCTAAAGTGTTTGTGGGGGAAAAGTACATTGGTGGAGCTGAAGAGATACGGCAAATGCATGAAGAGGGGAAGCTTGAGAAGTTGCTTGAATCTTGTGAAAAGTTGAGTGATGGCGGTGTAGGTGGTGGTAGCGTTTGTGAGGCTTGTGGGGATATAAGGTTTGTGCCATGTGAGACATGTTCCGGGAGCTGTAAAATTTACTGTGAAGACGATGAGCATGATCGAGGCGAAGAACATGAAGGCGAAGAAGAAGAAGGAGCAAAAGAGTGTGAGTGTGGATTCCAACGCTGCCCAGATTGTAATGAAAATGGCCTAATACGTTGCCCCATTTGTTGCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

313

Amino Acids

35.81

Weight (kDa)

4.67

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 142 - 210 1e-10 Glutaredoxin
GRXCR1-2_C PF23733 246 - 312 1.2e-18 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012964)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28850 AT5G39865
fragaria_vesca FvH4_6g48990
malus_domestica MD09G1049100.v1.1 MD17G1049100.v1.1
prunus_persica Prupe.3G269700_v2.0.a1
pyrus_communis pycom111g04010 pycom17g04410
rosa_chinensis RchiOBHm_Chr2g0168831
rosa_laevigata RLG00000021810
rosa_multiflora Rmu_ssc0000393.1_g000006
rosa_roxburghii Rroxscaffold_2G00082640
rosa_rugosa Rorug02G0535600
rosa_samantha Rh2AG606600 Rh2BG619400 Rh2CG588000 Rh2DG630000
rosa_wichuraiana Rw2G050280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 142, 155
AciI CCGC 4 cut(s) 62, 320, 454, 714
AclWI GGATC 2 cut(s) 134, 188
AcsI RAATTY 2 cut(s) 106, 795
AcuI CTGAAG 1 cut(s) 660
AfaI GTAC 2 cut(s) 431, 626
AfiI CCNNNNNNNGG 4 cut(s) 18, 19, 42, 484
AflIII ACRYGT 1 cut(s) 776
AgsI TTSAA 7 cut(s) 50, 89, 112, 295, 550, 562, 689
AhlI ACTAGT 1 cut(s) 437
AjuI GAANNNNNNNTTGG 4 cut(s) 378, 410, 612, 644
AluBI AGCT 8 cut(s) 181, 223, 268, 363, 568, 638, 673, 789
AluI AGCT 8 cut(s) 181, 223, 268, 363, 568, 638, 673, 789
Alw21I GWGCWC 3 cut(s) 183, 225, 270
Alw26I GTCTC 1 cut(s) 767
AlwI GGATC 2 cut(s) 134, 188
Ama87I CYCGRG 1 cut(s) 182
Aor13HI TCCGGA 2 cut(s) 142, 155
AoxI GGCC 2 cut(s) 63, 910
ApeKI GCWGC 1 cut(s) 888
ApoI RAATTY 2 cut(s) 106, 795
AspS9I GGNCC 1 cut(s) 148
AsuC2I CCSGG 1 cut(s) 784
AsuHPI GGTGA 1 cut(s) 499
AsuII TTCGAA 1 cut(s) 174
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 1 cut(s) 148
BanII GRGCYC 4 cut(s) 183, 225, 270, 315
BbsI GAAGAC 2 cut(s) 464, 813
Bbv12I GWGCWC 3 cut(s) 183, 225, 270
BbvI GCAGC 1 cut(s) 875
BccI CCATC 2 cut(s) 572, 704
BceAI ACGGC 1 cut(s) 665
BcnI CCSGG 1 cut(s) 784
BcoDI GTCTC 1 cut(s) 767
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 2 cut(s) 438, 569
BglI GCCNNNNNGGC 1 cut(s) 82
BisI GCNGC 2 cut(s) 63, 889
BlsI GCNGC 2 cut(s) 64, 890
Bme1390I CCNGG 1 cut(s) 784
Bme18I GGWCC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 148
BmrFI CCNGG 1 cut(s) 784
BmsI GCATC 1 cut(s) 525
BpiI GAAGAC 2 cut(s) 464, 813
BplI GAGNNNNNCTC 2 cut(s) 207, 239
Bpu10I CCTNAGC 1 cut(s) 72
Bpu14I TTCGAA 1 cut(s) 174
BpuEI CTTGAG 2 cut(s) 254, 695
BpuMI CCSGG 1 cut(s) 784
BsaJI CCNNGG 1 cut(s) 315
BsaWI WCCGGW 2 cut(s) 142, 155
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 19, 42, 484
BseAI TCCGGA 2 cut(s) 142, 155
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 4 cut(s) 238, 283, 328, 349
BseLI CCNNNNNNNGG 4 cut(s) 18, 19, 42, 484
BseRI GAGGAG 1 cut(s) 569
BseXI GCAGC 1 cut(s) 875
BshFI GGCC 2 cut(s) 65, 912
BsiHKAI GWGCWC 3 cut(s) 183, 225, 270
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 3 cut(s) 143, 156, 783
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 4 cut(s) 18, 19, 42, 484
BsmAI GTCTC 1 cut(s) 767
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 1 cut(s) 538
BsnI GGCC 2 cut(s) 65, 912
BsoBI CYCGRG 1 cut(s) 182
Bsp119I TTCGAA 1 cut(s) 174
Bsp1286I GDGCHC 4 cut(s) 183, 225, 270, 315
Bsp13I TCCGGA 2 cut(s) 142, 155
Bsp143I GATC 4 cut(s) 28, 139, 193, 820
BspACI CCGC 4 cut(s) 62, 320, 454, 714
BspANI GGCC 2 cut(s) 65, 912
BspEI TCCGGA 2 cut(s) 142, 155
BspPI GGATC 2 cut(s) 134, 188
BspQI GCTCTTC 4 cut(s) 171, 213, 258, 303
BspT104I TTCGAA 1 cut(s) 174
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 4 cut(s) 28, 139, 193, 820
BssT1I CCWWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 803
Bst6I CTCTTC 6 cut(s) 171, 213, 258, 303, 636, 657
BstBI TTCGAA 1 cut(s) 174
BstC8I GCNNGC 1 cut(s) 452
BstDEI CTNAG 2 cut(s) 72, 443
BstF5I GGATG 4 cut(s) 238, 283, 328, 349
BstKTI GATC 4 cut(s) 31, 142, 196, 823
BstMAI GTCTC 1 cut(s) 767
BstMBI GATC 4 cut(s) 28, 139, 193, 820
BstMWI GCNNNNNNNGC 1 cut(s) 82
BstNSI RCATGY 1 cut(s) 780
BstSCI CCNGG 1 cut(s) 782
BstV1I GCAGC 1 cut(s) 875
BstV2I GAAGAC 2 cut(s) 464, 813
BsuRI GGCC 2 cut(s) 65, 912
BtsCI GGATG 4 cut(s) 238, 283, 328, 349
Cac8I GCNNGC 1 cut(s) 452
Cfr13I GGNCC 1 cut(s) 148
Csp6I GTAC 2 cut(s) 430, 625
CviAII CATG 7 cut(s) 250, 479, 659, 768, 777, 818, 836
CviQI GTAC 2 cut(s) 430, 625
DdeI CTNAG 2 cut(s) 72, 443
DpnI GATC 4 cut(s) 30, 141, 195, 822
DpnII GATC 4 cut(s) 28, 139, 193, 820
DraI TTTAAA 1 cut(s) 208
Eam1104I CTCTTC 6 cut(s) 171, 213, 258, 303, 636, 657
EarI CTCTTC 6 cut(s) 171, 213, 258, 303, 636, 657
EciI GGCGGA 1 cut(s) 335
Ecl136II GAGCTC 3 cut(s) 181, 223, 268
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 4 cut(s) 183, 225, 270, 315
Eco47I GGWCC 1 cut(s) 148
Eco53kI GAGCTC 3 cut(s) 181, 223, 268
Eco57I CTGAAG 1 cut(s) 660
Eco88I CYCGRG 1 cut(s) 182
EcoICRI GAGCTC 3 cut(s) 181, 223, 268
EcoO109I RGGNCCY 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 315
EcoT22I ATGCAT 2 cut(s) 540, 660
EcoT38I GRGCYC 4 cut(s) 183, 225, 270, 315
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 7 cut(s) 253, 482, 662, 771, 780, 821, 839
FaqI GGGAC 1 cut(s) 18
FatI CATG 7 cut(s) 249, 478, 658, 767, 776, 817, 835
Fnu4HI GCNGC 2 cut(s) 63, 889
FokI GGATG 4 cut(s) 245, 290, 335, 356
FriOI GRGCYC 4 cut(s) 183, 225, 270, 315
Fsp4HI GCNGC 2 cut(s) 63, 889
FspBI CTAG 2 cut(s) 438, 569
GluI GCNGC 2 cut(s) 63, 889
HaeIII GGCC 2 cut(s) 65, 912
HapII CCGG 3 cut(s) 143, 156, 783
Hin1II CATG 7 cut(s) 253, 482, 662, 771, 780, 821, 839
HindIII AAGCTT 1 cut(s) 671
HinfI GANTC 7 cut(s) 127, 236, 281, 326, 490, 689, 879
HpaII CCGG 3 cut(s) 143, 156, 783
HphI GGTGA 1 cut(s) 499
Hpy188I TCNGA 2 cut(s) 343, 373
Hpy188III TCNNGA 3 cut(s) 50, 143, 156
HpyAV CCTTC 4 cut(s) 400, 556, 833, 848
HpyCH4III ACNGT 1 cut(s) 803
HpyCH4IV ACGT 1 cut(s) 919
HpyCH4V TGCA 2 cut(s) 538, 658
HpyF10VI GCNNNNNNNGC 1 cut(s) 82
HpyF3I CTNAG 2 cut(s) 72, 443
HpySE526I ACGT 1 cut(s) 919
Hsp92II CATG 7 cut(s) 253, 482, 662, 771, 780, 821, 839
Kpn2I TCCGGA 2 cut(s) 142, 155
Kzo9I GATC 4 cut(s) 28, 139, 193, 820
LguI GCTCTTC 4 cut(s) 171, 213, 258, 303
LmnI GCTCC 4 cut(s) 565, 635, 786, 857
LpnPI CCDG 6 cut(s) 4, 19, 156, 169, 796, 906
Lsp1109I GCAGC 1 cut(s) 875
LweI GCATC 1 cut(s) 525
MaeI CTAG 2 cut(s) 438, 569
MaeII ACGT 1 cut(s) 919
MalI GATC 4 cut(s) 30, 141, 195, 822
MboI GATC 4 cut(s) 28, 139, 193, 820
MfeI CAATTG 1 cut(s) 89
MhlI GDGCHC 4 cut(s) 183, 225, 270, 315
MluCI AATT 4 cut(s) 89, 106, 134, 795
MmeI TCCRAC 1 cut(s) 907
Mph1103I ATGCAT 2 cut(s) 540, 660
MroI TCCGGA 2 cut(s) 142, 155
MseI TTAA 1 cut(s) 207
MspI CCGG 3 cut(s) 143, 156, 783
MspR9I CCNGG 1 cut(s) 784
MunI CAATTG 1 cut(s) 89
Mva1269I GAATGC 1 cut(s) 538
MwoI GCNNNNNNNGC 1 cut(s) 82
NciI CCSGG 1 cut(s) 784
NdeII GATC 4 cut(s) 28, 139, 193, 820
NlaIII CATG 7 cut(s) 253, 482, 662, 771, 780, 821, 839
NsiI ATGCAT 2 cut(s) 540, 660
NspI RCATGY 1 cut(s) 780
NspV TTCGAA 1 cut(s) 174
PaeR7I CTCGAG 1 cut(s) 182
PciI ACATGT 1 cut(s) 776
PciSI GCTCTTC 4 cut(s) 171, 213, 258, 303
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 1 cut(s) 538
PfeI GAWTC 7 cut(s) 127, 236, 281, 326, 490, 689, 879
PfoI TCCNGGA 1 cut(s) 782
PkrI GCNGC 2 cut(s) 64, 890
PpuMI RGGWCCY 1 cut(s) 148
PscI ACATGT 1 cut(s) 776
Psp124BI GAGCTC 3 cut(s) 183, 225, 270
Psp5II RGGWCCY 1 cut(s) 148
PspPI GGNCC 1 cut(s) 148
PspPPI RGGWCCY 1 cut(s) 148
PspXI VCTCGAGB 1 cut(s) 182
RsaI GTAC 2 cut(s) 431, 626
RsaNI GTAC 2 cut(s) 430, 625
SacI GAGCTC 3 cut(s) 183, 225, 270
SapI GCTCTTC 4 cut(s) 171, 213, 258, 303
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 2 cut(s) 63, 889
Sau3AI GATC 4 cut(s) 28, 139, 193, 820
Sau96I GGNCC 1 cut(s) 148
ScrFI CCNGG 1 cut(s) 784
SduI GDGCHC 4 cut(s) 183, 225, 270, 315
SfaNI GCATC 1 cut(s) 525
Sfr274I CTCGAG 1 cut(s) 182
SfuI TTCGAA 1 cut(s) 174
SinI GGWCC 1 cut(s) 148
SlaI CTCGAG 1 cut(s) 182
SmlI CTYRAG 3 cut(s) 182, 269, 674
SmoI CTYRAG 3 cut(s) 182, 269, 674
SpeI ACTAGT 1 cut(s) 437
Sse9I AATT 4 cut(s) 89, 106, 134, 795
SsiI CCGC 4 cut(s) 62, 320, 454, 714
SspMI CTAG 2 cut(s) 438, 569
SstI GAGCTC 3 cut(s) 183, 225, 270
StyD4I CCNGG 1 cut(s) 782
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 1 cut(s) 803
TaiI ACGT 1 cut(s) 922
TaqI TCGA 4 cut(s) 174, 183, 533, 823
TasI AATT 4 cut(s) 89, 106, 134, 795
TatI WGTACW 2 cut(s) 429, 624
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 7 cut(s) 127, 236, 281, 326, 490, 689, 879
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TseI GCWGC 1 cut(s) 888
TspDTI ATGAA 9 cut(s) 191, 233, 238, 278, 355, 360, 675, 852, 918
VpaK11BI GGWCC 1 cut(s) 148
XapI RAATTY 2 cut(s) 106, 795
XceI RCATGY 1 cut(s) 780
XhoI CTCGAG 1 cut(s) 182
XspI CTAG 2 cut(s) 438, 569
Zsp2I ATGCAT 2 cut(s) 540, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.