Rroxscaffold_2G00102240

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008754
Physical Location & Seq
Forward (+)
24288754 .. 24293157
4404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00102240.1

Sequence Viewer

Length: 1464 bp
ATGGACAATTTGTTGCTGACTGTACTAGTCTGTAGTACTACCATATTCTTGTCCACAGCACTAATTCTCTCCAGAAACTACTTCAGGTTTAGTCCAGTCTGTTTCAATATCAGATCATCAAAACACAGAAGACTCTCACTTCCTTTAGGCACTCTTGGATGGCCGTTTCTCGGCGAAACCATTGAGTTTGTGTCTTGTGCTTACTCTGATTGCCCCGAGAGCTTCATGAATAAACGTCGTCATAGGTATGGAAAGGTGTTTAAATCACACATATTTGGGAGTCCAACTATTGTTTCAACAGATGCAGAAGTGAGTAAGTTTGTTCTGCAAAGTGATGCCAAGGCTTTTGTTCCATCTTACCCAAAATCTCTCACAGAGTTGATGGGGAAGTCTTCTATATTGCTCATCAATGGAAGCTTGCAAAGAAGAATCCATGGACTCATAGGAGCTTTCTTTAAGTCTCCACACCTCAAAGCTCAAATCACCAGAGACATGCAGAAGTATGTCCAAGAATCAATGGCAACTTGGAGAGATGACCTTCCCATTTTCATTCAAGATGAAACCAAAAATATTGCATTTCAAGTACTTGTCAAAGCATTGATTAGTCTGGATCCTGGTGAGGACATGGAGTTTCTAAAGAAACAGTTCCAAGAATTCATTGCTGGCCTCATGTCTATACCCATCAATATTCCTGGAAGTAGACTTTACAGATCATTACAGGCCAAGAAGAAAATGGTCAAGCTAGTCCAGAAAATTATACAAGCTAAAAAGGAGAGTGGGATCTCCAATGTTTCCCAAGATGTGGTGGATGTTTTACTTAATGACACAAGTGATCAATTAACAGATGATCTTATAGCAGATAACATGATTGACATGATGATACCAGGAGAGGATTCAGTACCACTTCTTATGACTCTTGCAATCAAATACCTTTCAGATTCCCCCACTGCTCTTCAACAGTTAACGAAGGAGAACATGGAGTTAAAAACACTCAAAGAGCAGCTTGGAGAGCCATTGTGTTGGAGTGATTACTTATCCTTACCATTTACACATAATGTGATTACAGAAACTCTAAGGATGGGAAACATCATAAATGGGGTGATGAGGAAGGCCATGAAAGATGTTGAGATAAAAGGCTATCAAATACCAAAGGGGTGGTGTGTCTTCACATACTTTAGATCAGTTCATCTTGATGAGAATAACTATGATGGGGCTTATGACTTCAATCCATGGAGATGGCAAGACAAAGACATAAGCAGCTGTAACTTCACTCCTTTTGGAGGTGGACAAAGACTTTGTCCTGGGCTTGACTTGGCTAGGCTGGAAGCTTCCATCTTCCTACACCACTTTATCACTCAGTTCAGTTGGATGGCTGAGGATGACACAATTGTAAACTTCCCCACTGTAAGAATGAAGAGGAGGATGCCAGTCTGGGTCAAAAGGAGAAGGGTCGACCAGTACTAA

Protein Analysis

487

Amino Acids

55.79

Weight (kDa)

8.82

Isoelectric Point (pI)

51.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 49 - 462 6.5e-52 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 802
AccI GTMKAC 2 cut(s) 700, 1452
AclWI GGATC 3 cut(s) 605, 618, 788
AcoI YGGCCR 1 cut(s) 161
AcsI RAATTY 1 cut(s) 653
AcuI CTGAAG 1 cut(s) 67
AfaI GTAC 5 cut(s) 24, 37, 585, 900, 1460
AfiI CCNNNNNNNGG 3 cut(s) 170, 802, 1280
AgsI TTSAA 6 cut(s) 106, 297, 554, 581, 956, 1225
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 4 cut(s) 613, 691, 883, 1300
AjuI GAANNNNNNNTTGG 2 cut(s) 277, 309
AluBI AGCT 9 cut(s) 222, 417, 449, 476, 742, 764, 1003, 1260, 1328
AluI AGCT 9 cut(s) 222, 417, 449, 476, 742, 764, 1003, 1260, 1328
Alw26I GTCTC 2 cut(s) 465, 483
AlwI GGATC 3 cut(s) 605, 618, 788
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 4 cut(s) 161, 664, 720, 1110
ApeKI GCWGC 2 cut(s) 1000, 1257
ApoI RAATTY 1 cut(s) 653
ArsI GACNNNNNNTTYG 4 cut(s) 1278, 1282, 1310, 1314
Asp700I GAANNNNTTC 1 cut(s) 644
AsuHPI GGTGA 3 cut(s) 475, 629, 1111
AvaI CYCGRG 1 cut(s) 215
BamHI GGATCC 1 cut(s) 610
BbsI GAAGAC 3 cut(s) 136, 384, 1156
BbvCI CCTCAGC 1 cut(s) 1374
BbvI GCAGC 2 cut(s) 1012, 1269
BccI CCATC 9 cut(s) 153, 361, 376, 689, 1072, 1202, 1230, 1340, 1363
BceAI ACGGC 1 cut(s) 148
BciT130I CCWGG 4 cut(s) 615, 693, 885, 1302
BclI TGATCA 1 cut(s) 832
BcoDI GTCTC 2 cut(s) 465, 483
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 3 cut(s) 26, 743, 1317
BfmI CTRYAG 1 cut(s) 31
BisI GCNGC 2 cut(s) 1001, 1258
BlsI GCNGC 2 cut(s) 1002, 1259
BmcAI AGTACT 3 cut(s) 37, 585, 1460
Bme1390I CCNGG 4 cut(s) 615, 693, 885, 1302
BmeT110I CYCGRG 1 cut(s) 215
BmiI GGNNCC 1 cut(s) 612
BmrFI CCNGG 4 cut(s) 615, 693, 885, 1302
BmsI GCATC 3 cut(s) 292, 325, 1413
BpiI GAAGAC 3 cut(s) 136, 384, 1156
BpmI CTGGAG 1 cut(s) 55
Bpu10I CCTNAGC 1 cut(s) 1374
BsaJI CCNNGG 4 cut(s) 339, 433, 1229, 1301
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 802, 1280
Bse1I ACTGG 3 cut(s) 95, 1427, 1456
Bse3DI GCAATG 1 cut(s) 657
BseBI CCWGG 4 cut(s) 615, 693, 885, 1302
BseDI CCNNGG 4 cut(s) 339, 433, 1229, 1301
BseGI GGATG 6 cut(s) 164, 814, 1083, 1374, 1384, 1428
BseLI CCNNNNNNNGG 3 cut(s) 170, 802, 1280
BseMI GCAATG 1 cut(s) 657
BseMII CTCAG 2 cut(s) 1365, 1370
BseNI ACTGG 3 cut(s) 95, 1427, 1456
BseRI GAGGAG 1 cut(s) 1432
BseXI GCAGC 2 cut(s) 1012, 1269
BshFI GGCC 4 cut(s) 163, 666, 722, 1112
BsiHKCI CYCGRG 1 cut(s) 215
BslI CCNNNNNNNGG 3 cut(s) 170, 802, 1280
BsmAI GTCTC 2 cut(s) 465, 483
BsnI GGCC 4 cut(s) 163, 666, 722, 1112
BsoBI CYCGRG 1 cut(s) 215
Bsp143I GATC 7 cut(s) 113, 610, 710, 780, 832, 847, 1178
Bsp19I CCATGG 2 cut(s) 433, 1229
BspANI GGCC 4 cut(s) 163, 666, 722, 1112
BspCNI CTCAG 2 cut(s) 1366, 1369
BspHI TCATGA 1 cut(s) 225
BspLI GGNNCC 1 cut(s) 612
BspPI GGATC 3 cut(s) 605, 618, 788
BspQI GCTCTTC 1 cut(s) 957
BsrDI GCAATG 1 cut(s) 657
BsrI ACTGG 3 cut(s) 95, 1427, 1456
BssECI CCNNGG 4 cut(s) 339, 433, 1229, 1301
BssMI GATC 7 cut(s) 113, 610, 710, 780, 832, 847, 1178
BssT1I CCWWGG 3 cut(s) 339, 433, 1229
Bst2UI CCWGG 4 cut(s) 615, 693, 885, 1302
Bst4CI ACNGT 4 cut(s) 22, 645, 960, 1405
Bst6I CTCTTC 2 cut(s) 957, 1409
BstC8I GCNNGC 2 cut(s) 419, 664
BstDEI CTNAG 3 cut(s) 1073, 1356, 1374
BstDSI CCRYGG 2 cut(s) 433, 1229
BstENI CCTNNNNNAGG 1 cut(s) 1278
BstF5I GGATG 6 cut(s) 164, 814, 1083, 1374, 1384, 1428
BstKTI GATC 7 cut(s) 116, 613, 713, 783, 835, 850, 1181
BstMAI GTCTC 2 cut(s) 465, 483
BstMBI GATC 7 cut(s) 113, 610, 710, 780, 832, 847, 1178
BstMWI GCNNNNNNNGC 2 cut(s) 219, 1009
BstNI CCWGG 4 cut(s) 615, 693, 885, 1302
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 4 cut(s) 613, 691, 883, 1300
BstSFI CTRYAG 1 cut(s) 31
BstV1I GCAGC 2 cut(s) 1012, 1269
BstV2I GAAGAC 3 cut(s) 136, 384, 1156
BstX2I RGATCY 2 cut(s) 610, 780
BstXI CCANNNNNNTGG 3 cut(s) 1020, 1155, 1236
BstYI RGATCY 2 cut(s) 610, 780
BsuRI GGCC 4 cut(s) 163, 666, 722, 1112
BtgI CCRYGG 2 cut(s) 433, 1229
BtsCI GGATG 6 cut(s) 164, 814, 1083, 1374, 1384, 1428
BtsI GCAGTG 1 cut(s) 945
BtsIMutI CAGTG 2 cut(s) 945, 1401
Cac8I GCNNGC 2 cut(s) 419, 664
CciI TCATGA 1 cut(s) 225
Csp6I GTAC 5 cut(s) 23, 36, 584, 899, 1459
CviQI GTAC 5 cut(s) 23, 36, 584, 899, 1459
DdeI CTNAG 3 cut(s) 1073, 1356, 1374
DpnI GATC 7 cut(s) 115, 612, 712, 782, 834, 849, 1180
DpnII GATC 7 cut(s) 113, 610, 710, 780, 832, 847, 1178
DraI TTTAAA 1 cut(s) 262
EaeI YGGCCR 1 cut(s) 161
Eam1104I CTCTTC 2 cut(s) 957, 1409
EarI CTCTTC 2 cut(s) 957, 1409
Eco130I CCWWGG 3 cut(s) 339, 433, 1229
Eco57I CTGAAG 1 cut(s) 67
Eco88I CYCGRG 1 cut(s) 215
EcoNI CCTNNNNNAGG 1 cut(s) 1278
EcoRI GAATTC 1 cut(s) 653
EcoRII CCWGG 4 cut(s) 613, 691, 883, 1300
EcoT14I CCWWGG 3 cut(s) 339, 433, 1229
ErhI CCWWGG 3 cut(s) 339, 433, 1229
FalI AAGNNNNNCTT 2 cut(s) 987, 1019
FbaI TGATCA 1 cut(s) 832
FblI GTMKAC 2 cut(s) 700, 1452
Fnu4HI GCNGC 2 cut(s) 1001, 1258
FokI GGATG 6 cut(s) 171, 821, 1090, 1381, 1391, 1435
Fsp4HI GCNGC 2 cut(s) 1001, 1258
FspBI CTAG 3 cut(s) 26, 743, 1317
GluI GCNGC 2 cut(s) 1001, 1258
GsuI CTGGAG 1 cut(s) 55
HaeIII GGCC 4 cut(s) 163, 666, 722, 1112
HincII GTYRAC 2 cut(s) 963, 1453
HindII GTYRAC 2 cut(s) 963, 1453
HindIII AAGCTT 2 cut(s) 415, 1326
HinfI GANTC 8 cut(s) 132, 280, 429, 438, 512, 893, 913, 938
HpaI GTTAAC 1 cut(s) 963
HphI GGTGA 3 cut(s) 475, 629, 1111
Hpy166II GTNNAC 6 cut(s) 54, 701, 963, 1286, 1393, 1453
Hpy188I TCNGA 3 cut(s) 113, 208, 937
Hpy188III TCNNGA 6 cut(s) 72, 226, 554, 608, 748, 1190
Hpy8I GTNNAC 6 cut(s) 54, 701, 963, 1286, 1393, 1453
Hpy99I CGWCG 1 cut(s) 240
HpyAV CCTTC 4 cut(s) 548, 961, 1102, 1440
HpyCH4III ACNGT 4 cut(s) 22, 645, 960, 1405
HpyCH4IV ACGT 1 cut(s) 235
HpyCH4V TGCA 6 cut(s) 305, 328, 421, 496, 575, 920
HpyF10VI GCNNNNNNNGC 2 cut(s) 219, 1009
HpyF3I CTNAG 3 cut(s) 1073, 1356, 1374
HpySE526I ACGT 1 cut(s) 235
Ksp22I TGATCA 1 cut(s) 832
KspAI GTTAAC 1 cut(s) 963
Kzo9I GATC 7 cut(s) 113, 610, 710, 780, 832, 847, 1178
LguI GCTCTTC 1 cut(s) 957
LmnI GCTCC 1 cut(s) 446
Lsp1109I GCAGC 2 cut(s) 1012, 1269
LweI GCATC 3 cut(s) 292, 325, 1413
MaeI CTAG 3 cut(s) 26, 743, 1317
MaeII ACGT 1 cut(s) 235
MaeIII GTNAC 1 cut(s) 1262
MalI GATC 7 cut(s) 115, 612, 712, 782, 834, 849, 1180
MboI GATC 7 cut(s) 113, 610, 710, 780, 832, 847, 1178
MboII GAAGA 8 cut(s) 141, 384, 438, 739, 944, 1156, 1327, 1426
MfeI CAATTG 1 cut(s) 1386
MflI RGATCY 2 cut(s) 610, 780
MluCI AATT 6 cut(s) 7, 63, 653, 753, 836, 1386
MlyI GAGTC 4 cut(s) 126, 289, 432, 907
MmeI TCCRAC 3 cut(s) 308, 1001, 1346
MnlI CCTC 9 cut(s) 479, 613, 677, 883, 1098, 1274, 1369, 1410, 1413
MroXI GAANNNNTTC 1 cut(s) 644
MseI TTAA 6 cut(s) 261, 456, 819, 839, 962, 983
MslI CAYNNNNRTG 3 cut(s) 246, 1191, 1234
MspA1I CMGCKG 1 cut(s) 1260
MspR9I CCNGG 4 cut(s) 615, 693, 885, 1302
MunI CAATTG 1 cut(s) 1386
MvaI CCWGG 4 cut(s) 615, 693, 885, 1302
MwoI GCNNNNNNNGC 2 cut(s) 219, 1009
NcoI CCATGG 2 cut(s) 433, 1229
NdeII GATC 7 cut(s) 113, 610, 710, 780, 832, 847, 1178
NlaIV GGNNCC 1 cut(s) 612
NmeAIII GCCGAG 1 cut(s) 150
NspI RCATGY 1 cut(s) 496
PagI TCATGA 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 957
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 4 cut(s) 429, 512, 893, 938
PflFI GACNNNGTC 1 cut(s) 1296
PflMI CCANNNNNTGG 1 cut(s) 802
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 2 cut(s) 1002, 1259
PleI GAGTC 4 cut(s) 126, 288, 432, 907
PpsI GAGTC 4 cut(s) 126, 288, 432, 907
Psp6I CCWGG 4 cut(s) 613, 691, 883, 1300
PspGI CCWGG 4 cut(s) 613, 691, 883, 1300
PspN4I GGNNCC 1 cut(s) 612
PsuI RGATCY 2 cut(s) 610, 780
PsyI GACNNNGTC 1 cut(s) 1296
PvuII CAGCTG 1 cut(s) 1260
RsaI GTAC 5 cut(s) 24, 37, 585, 900, 1460
RsaNI GTAC 5 cut(s) 23, 36, 584, 899, 1459
RseI CAYNNNNRTG 3 cut(s) 246, 1191, 1234
SalI GTCGAC 1 cut(s) 1451
SapI GCTCTTC 1 cut(s) 957
SaqAI TTAA 6 cut(s) 261, 456, 819, 839, 962, 983
SatI GCNGC 2 cut(s) 1001, 1258
Sau3AI GATC 7 cut(s) 113, 610, 710, 780, 832, 847, 1178
ScaI AGTACT 3 cut(s) 37, 585, 1460
SchI GAGTC 4 cut(s) 126, 289, 432, 907
ScrFI CCNGG 4 cut(s) 615, 693, 885, 1302
SfaNI GCATC 3 cut(s) 292, 325, 1413
SfcI CTRYAG 1 cut(s) 31
SmiMI CAYNNNNRTG 3 cut(s) 246, 1191, 1234
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 6 cut(s) 7, 63, 653, 753, 836, 1386
SspI AATATT 2 cut(s) 571, 688
SspMI CTAG 3 cut(s) 26, 743, 1317
StyD4I CCNGG 4 cut(s) 613, 691, 883, 1300
StyI CCWWGG 3 cut(s) 339, 433, 1229
TaaI ACNGT 4 cut(s) 22, 645, 960, 1405
TaiI ACGT 1 cut(s) 238
TaqI TCGA 1 cut(s) 1452
TasI AATT 6 cut(s) 7, 63, 653, 753, 836, 1386
TatI WGTACW 4 cut(s) 22, 35, 583, 1458
TfiI GAWTC 4 cut(s) 429, 512, 893, 938
Tru1I TTAA 6 cut(s) 261, 456, 819, 839, 962, 983
Tru9I TTAA 6 cut(s) 261, 456, 819, 839, 962, 983
TscAI CASTG 2 cut(s) 952, 1408
TseI GCWGC 2 cut(s) 1000, 1257
TspDTI ATGAA 8 cut(s) 214, 242, 538, 573, 646, 1130, 1175, 1427
TspRI CASTG 2 cut(s) 952, 1408
Tth111I GACNNNGTC 1 cut(s) 1296
Van91I CCANNNNNTGG 1 cut(s) 802
XagI CCTNNNNNAGG 1 cut(s) 1278
XapI RAATTY 1 cut(s) 653
XceI RCATGY 1 cut(s) 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
XmiI GTMKAC 2 cut(s) 700, 1452
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 3 cut(s) 26, 743, 1317
ZrmI AGTACT 3 cut(s) 37, 585, 1460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.