Rroxscaffold_2G00126230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0019422
Physical Location & Seq
Reverse (-)
61354299 .. 61355997
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126230.1

Sequence Viewer

Length: 597 bp
ATGCCATCGGAGGTGGAGAAAGGAGAGGCGAAGTCGTCTTCATCGTCATCGTCAATGACGACATTTTGGGGAGAGGAGGACGGGTTGCCTCCGGCATCGTCTTGGTCGGGGGCCATGGTGGTGGTGAGGCAATTGCTTACTCTTGAAGAGCACGAACGTGGTCTTACCATCATCAAGCTAATGCATGTGGATGTTCTGAAGAAGACAGGGCTTGCCGAGTGCCTAGAAGCCATCTACAAGCTCTACACCTGGGAGATGGTGGTTGTATCACCACAACATATACTAGATAGGGCTCCAAGAGCAAAGGATCAAAGTAAACAGGAGAAGAATAAAGGCCTGGGTTCACATAAGATATTCCCCAGCAGCAACGCTCCAAAGGGTTTGGTACAGTCGAAGCTCTCACGCCCCCAGTGGGAGTATTATTACAGAGACCCACAATGTGGTGAGGCTGTTAGTGCCATGCCTCTTCTTCTTCTTCTTTTACCTGAACTGAGACGGAGACAGGCCAGAGCTGAGAGCCCAGAGGCAGCAGCTGGGCGTGCAGAGAGAGCCTGCATGCTTGCTTTCCTCATATGCATGGAGATGCATGGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.07

Weight (kDa)

7.69

Isoelectric Point (pI)

73.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019422)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0010411
rosa_laevigata RLG00000036650 RLG00000036654
rosa_multiflora Rmu_sc0003323.1_g000010
rosa_roxburghii Rroxscaffold_2G00126230
rosa_rugosa Rorug05G0450700
rosa_samantha Rh2CG286000
rosa_wichuraiana Rw5G047110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 440
AclWI GGATC 1 cut(s) 315
AcuI CTGAAG 1 cut(s) 218
AdeI CACNNNGTG 1 cut(s) 440
AfaI GTAC 1 cut(s) 387
AfiI CCNNNNNNNGG 2 cut(s) 412, 440
AgsI TTSAA 1 cut(s) 146
AjnI CCWGG 2 cut(s) 248, 336
AleI CACNNNNGTG 1 cut(s) 156
AluBI AGCT 5 cut(s) 178, 241, 397, 512, 533
AluI AGCT 5 cut(s) 178, 241, 397, 512, 533
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 3 cut(s) 423, 487, 493
AlwI GGATC 1 cut(s) 315
AlwNI CAGNNNCTG 1 cut(s) 533
AoxI GGCC 3 cut(s) 111, 334, 504
ApeKI GCWGC 3 cut(s) 363, 527, 530
AspS9I GGNCC 1 cut(s) 111
AsuHPI GGTGA 3 cut(s) 136, 261, 455
BanII GRGCYC 2 cut(s) 295, 521
BbsI GAAGAC 2 cut(s) 30, 209
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 3 cut(s) 375, 539, 542
BccI CCATC 4 cut(s) 13, 176, 239, 250
BciT130I CCWGG 2 cut(s) 250, 338
BcoDI GTCTC 3 cut(s) 423, 487, 493
BfaI CTAG 2 cut(s) 224, 284
BisI GCNGC 3 cut(s) 364, 528, 531
BlsI GCNGC 3 cut(s) 365, 529, 532
Bme1390I CCNGG 2 cut(s) 250, 338
BmgT120I GGNCC 1 cut(s) 111
BmiI GGNNCC 2 cut(s) 112, 294
BmrFI CCNGG 2 cut(s) 250, 338
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 2 cut(s) 104, 573
BmuI ACTGGG 1 cut(s) 403
BpiI GAAGAC 2 cut(s) 30, 209
BsaI GGTCTC 1 cut(s) 423
BsaJI CCNNGG 3 cut(s) 114, 249, 337
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 440
Bse1I ACTGG 1 cut(s) 409
BseBI CCWGG 2 cut(s) 250, 338
BseDI CCNNGG 3 cut(s) 114, 249, 337
BseGI GGATG 1 cut(s) 196
BseLI CCNNNNNNNGG 2 cut(s) 412, 440
BseMII CTCAG 2 cut(s) 482, 504
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 1 cut(s) 89
BseXI GCAGC 3 cut(s) 375, 539, 542
BseYI CCCAGC 2 cut(s) 359, 533
BsgI GTGCAG 1 cut(s) 561
BshFI GGCC 3 cut(s) 113, 336, 506
BsiHKAI GWGCWC 1 cut(s) 153
BsiSI CCGG 1 cut(s) 92
BslI CCNNNNNNNGG 2 cut(s) 412, 440
BsmAI GTCTC 3 cut(s) 423, 487, 493
BsmBI CGTCTC 1 cut(s) 487
BsnI GGCC 3 cut(s) 113, 336, 506
Bso31I GGTCTC 1 cut(s) 423
Bsp1286I GDGCHC 3 cut(s) 153, 295, 521
Bsp143I GATC 1 cut(s) 307
Bsp19I CCATGG 1 cut(s) 114
BspANI GGCC 3 cut(s) 113, 336, 506
BspCNI CTCAG 2 cut(s) 483, 505
BspLI GGNNCC 2 cut(s) 112, 294
BspPI GGATC 1 cut(s) 315
BspQI GCTCTTC 1 cut(s) 141
BspTNI GGTCTC 1 cut(s) 423
BsrI ACTGG 1 cut(s) 409
BssECI CCNNGG 3 cut(s) 114, 249, 337
BssMI GATC 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 250, 338
Bst4CI ACNGT 1 cut(s) 390
Bst6I CTCTTC 2 cut(s) 141, 471
BstC8I GCNNGC 5 cut(s) 213, 540, 553, 557, 561
BstDEI CTNAG 2 cut(s) 491, 513
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 196
BstKTI GATC 1 cut(s) 310
BstMAI GTCTC 3 cut(s) 423, 487, 493
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 4 cut(s) 299, 455, 539, 548
BstNI CCWGG 2 cut(s) 250, 338
BstNSI RCATGY 2 cut(s) 188, 559
BstSCI CCNGG 2 cut(s) 248, 336
BstV1I GCAGC 3 cut(s) 375, 539, 542
BstV2I GAAGAC 2 cut(s) 30, 209
BstXI CCANNNNNNTGG 1 cut(s) 121
BsuRI GGCC 3 cut(s) 113, 336, 506
BtgI CCRYGG 1 cut(s) 114
BtsCI GGATG 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 416
Cac8I GCNNGC 5 cut(s) 213, 540, 553, 557, 561
CaiI CAGNNNCTG 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 111
Csp6I GTAC 1 cut(s) 386
CviAII CATG 7 cut(s) 115, 185, 460, 556, 577, 587, 594
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 2 cut(s) 491, 513
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
DraIII CACNNNGTG 1 cut(s) 440
Eam1104I CTCTTC 2 cut(s) 141, 471
EarI CTCTTC 2 cut(s) 141, 471
Eco130I CCWWGG 1 cut(s) 114
Eco147I AGGCCT 1 cut(s) 336
Eco24I GRGCYC 2 cut(s) 295, 521
Eco31I GGTCTC 1 cut(s) 423
Eco57I CTGAAG 1 cut(s) 218
EcoRII CCWGG 2 cut(s) 248, 336
EcoT14I CCWWGG 1 cut(s) 114
EcoT22I ATGCAT 3 cut(s) 186, 578, 588
EcoT38I GRGCYC 2 cut(s) 295, 521
ErhI CCWWGG 1 cut(s) 114
Esp3I CGTCTC 1 cut(s) 487
FaeI CATG 7 cut(s) 118, 188, 463, 559, 580, 590, 597
FatI CATG 7 cut(s) 114, 184, 459, 555, 576, 586, 593
FauNDI CATATG 1 cut(s) 572
Fnu4HI GCNGC 3 cut(s) 364, 528, 531
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 2 cut(s) 295, 521
Fsp4HI GCNGC 3 cut(s) 364, 528, 531
FspBI CTAG 2 cut(s) 224, 284
GluI GCNGC 3 cut(s) 364, 528, 531
GsaI CCCAGC 2 cut(s) 363, 537
HaeIII GGCC 3 cut(s) 113, 336, 506
HapII CCGG 1 cut(s) 92
Hin1II CATG 7 cut(s) 118, 188, 463, 559, 580, 590, 597
HpaII CCGG 1 cut(s) 92
HphI GGTGA 3 cut(s) 136, 261, 455
Hpy166II GTNNAC 2 cut(s) 317, 344
Hpy188I TCNGA 2 cut(s) 10, 198
Hpy188III TCNNGA 1 cut(s) 143
Hpy8I GTNNAC 2 cut(s) 317, 344
HpyCH4III ACNGT 1 cut(s) 390
HpyCH4IV ACGT 1 cut(s) 157
HpyCH4V TGCA 5 cut(s) 184, 542, 555, 576, 586
HpyF10VI GCNNNNNNNGC 4 cut(s) 299, 455, 539, 548
HpyF3I CTNAG 2 cut(s) 491, 513
HpySE526I ACGT 1 cut(s) 157
Hsp92II CATG 7 cut(s) 118, 188, 463, 559, 580, 590, 597
Kzo9I GATC 1 cut(s) 307
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 2 cut(s) 298, 376
Lsp1109I GCAGC 3 cut(s) 375, 539, 542
LweI GCATC 2 cut(s) 104, 573
MaeI CTAG 2 cut(s) 224, 284
MaeII ACGT 1 cut(s) 157
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 9 cut(s) 30, 158, 211, 214, 337, 458, 461, 464, 467
MfeI CAATTG 1 cut(s) 131
MhlI GDGCHC 3 cut(s) 153, 295, 521
MluCI AATT 1 cut(s) 131
Mph1103I ATGCAT 3 cut(s) 186, 578, 588
MslI CAYNNNNRTG 5 cut(s) 119, 156, 189, 575, 581
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 2 cut(s) 250, 338
MunI CAATTG 1 cut(s) 131
MvaI CCWGG 2 cut(s) 250, 338
MwoI GCNNNNNNNGC 4 cut(s) 299, 455, 539, 548
NcoI CCATGG 1 cut(s) 114
NdeI CATATG 1 cut(s) 572
NdeII GATC 1 cut(s) 307
NlaIII CATG 7 cut(s) 118, 188, 463, 559, 580, 590, 597
NlaIV GGNNCC 2 cut(s) 112, 294
NmeAIII GCCGAG 1 cut(s) 241
NsiI ATGCAT 3 cut(s) 186, 578, 588
NspI RCATGY 2 cut(s) 188, 559
OliI CACNNNNGTG 1 cut(s) 156
PaeI GCATGC 1 cut(s) 559
PceI AGGCCT 1 cut(s) 336
PciSI GCTCTTC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 2 cut(s) 41, 56
PflMI CCANNNNNTGG 1 cut(s) 440
PkrI GCNGC 3 cut(s) 365, 529, 532
Psp6I CCWGG 2 cut(s) 248, 336
PspFI CCCAGC 2 cut(s) 359, 533
PspGI CCWGG 2 cut(s) 248, 336
PspN4I GGNNCC 2 cut(s) 112, 294
PspPI GGNCC 1 cut(s) 111
PstNI CAGNNNCTG 1 cut(s) 533
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
RseI CAYNNNNRTG 5 cut(s) 119, 156, 189, 575, 581
SapI GCTCTTC 1 cut(s) 141
SatI GCNGC 3 cut(s) 364, 528, 531
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 1 cut(s) 111
ScrFI CCNGG 2 cut(s) 250, 338
SduI GDGCHC 3 cut(s) 153, 295, 521
SetI ASST 9 cut(s) 15, 160, 180, 243, 251, 399, 487, 514, 535
SfaNI GCATC 2 cut(s) 104, 573
SmiMI CAYNNNNRTG 5 cut(s) 119, 156, 189, 575, 581
SphI GCATGC 1 cut(s) 559
Sse9I AATT 1 cut(s) 131
SseBI AGGCCT 1 cut(s) 336
SspMI CTAG 2 cut(s) 224, 284
StuI AGGCCT 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 248, 336
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 1 cut(s) 390
TaiI ACGT 1 cut(s) 160
TaqI TCGA 1 cut(s) 392
TasI AATT 1 cut(s) 131
TscAI CASTG 1 cut(s) 416
TseI GCWGC 3 cut(s) 363, 527, 530
TspDTI ATGAA 1 cut(s) 30
TspGWI ACGGA 1 cut(s) 511
TspRI CASTG 1 cut(s) 416
Van91I CCANNNNNTGG 1 cut(s) 440
XceI RCATGY 2 cut(s) 188, 559
XspI CTAG 2 cut(s) 224, 284
Zsp2I ATGCAT 3 cut(s) 186, 578, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.