Rroxscaffold_2G00132480

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017224
Physical Location & Seq
Reverse (-)
69487975 .. 69489293
1319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00132480.1

Sequence Viewer

Length: 885 bp
ATGGATGATCCGAGCAGCAGTGGCGTACCCGAGTCCGAAAAGCTTCTCGGAATCGCCGCCGATCTCCTTTGCGACGGCGACTTCGCTTCTTGCCGGAAATTCGCTCTCTTCGCCCGCGACTACGGCCCGGAAAACCCCGTCGTGGAAAGAATCCTCGCCGTCGCCGACGTTCTCCTAGCCGAGAAGAGGCGCGACCCGACCGACTGGTACCCCATTTTGCAACTGACCCGACCCGAATCCGGAAACCGCCGCCTCGTTCGGGCCCAGTTCGAGAAGCTGACGGCGCTGTTGGACCCGGAAGACAACGGCTTCCCGTTTGCCGACGAGGCGCTCGGGCTGGTCCACAAGGCGTGGGCCGCGCTGTCCGACCCGTGGTTCGGAAATGGGCGCAAGAACGGGCCGGAAAGCCCGAAACTTGAAGAGGGTTTGGCCGGAGAGACGTTCTGGACGGTTTGTCCGTACTGCTATGGCATGTTTATGTACAAGAAGGTGTTTGAGGAGTGTTGCCTGAGGTGTCAGATTTGCCGGAAGGCGTTTCACGGGGTGGCGATTAGGCCGCCGGCGCCGGAGATTTTGGTGGAGGGGAAGGAGCAGTACTATTTTACTTATGGGTGTTTTCCCATGGAGTACAATGAGGAACCAAAGAAGAGTAATGAGGACACAAAGAAGAGGCAGATGGAGGAGGCTGTTAGGGTTGTGGAGATTTCTGATGATGATGAGGAGGTGGTGGTGGTGAAGAATGTGGGTGGAGGAGGGAAGTTTTCGAGCGAAGGGAAGGCTCCGGTGAAGAGAATGAAGGCTCCGGCGAGGCGGATGAAGGGGATGAAGACGAAAACAGTGGCGAGTAGGAGGGGTGGGATGGTGAAAGAGTTGGACTTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

294

Amino Acids

32.99

Weight (kDa)

5.74

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017224)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19770
malus_domestica MD15G1313800.v1.1
prunus_persica Prupe.6G198600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0111341
rosa_laevigata RLG00000017887
rosa_multiflora Rmu_co8456405.1_g000001
rosa_roxburghii Rroxscaffold_2G00132480
rosa_rugosa Rorug02G0172800
rosa_samantha Rh2AG226000 Rh2BG237800 Rh2CG229000 Rh2DG233500
rosa_wichuraiana Rw2G017450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 207
AccB1I GGYRCC 2 cut(s) 207, 562
AccII CGCG 3 cut(s) 117, 192, 359
AccIII TCCGGA 1 cut(s) 239
AciI CCGC 7 cut(s) 57, 115, 247, 250, 357, 557, 811
AclWI GGATC 1 cut(s) 2
AcoI YGGCCR 1 cut(s) 429
AcsI RAATTY 1 cut(s) 98
AcyI GRCGYC 1 cut(s) 563
AdeI CACNNNGTG 1 cut(s) 544
AfaI GTAC 6 cut(s) 27, 209, 461, 482, 596, 629
AfiI CCNNNNNNNGG 6 cut(s) 142, 186, 239, 259, 372, 377
AgsI TTSAA 2 cut(s) 419, 880
AhdI GACNNNNNGTC 1 cut(s) 453
AluBI AGCT 2 cut(s) 43, 277
AluI AGCT 2 cut(s) 43, 277
Alw26I GTCTC 1 cut(s) 431
AlwI GGATC 1 cut(s) 2
Ama87I CYCGRG 2 cut(s) 29, 332
Aor13HI TCCGGA 1 cut(s) 239
AoxI GGCC 6 cut(s) 124, 261, 354, 398, 429, 554
ApaI GGGCCC 1 cut(s) 265
ApeKI GCWGC 1 cut(s) 15
ApoI RAATTY 1 cut(s) 98
ArsI GACNNNNNNTTYG 4 cut(s) 65, 97, 359, 391
Asp700I GAANNNNTTC 1 cut(s) 42
Asp718I GGTACC 1 cut(s) 207
AspLEI GCGC 6 cut(s) 192, 286, 331, 361, 390, 565
AspS9I GGNCC 7 cut(s) 125, 261, 262, 292, 340, 354, 398
AsuC2I CCSGG 2 cut(s) 128, 296
AsuHPI GGTGA 3 cut(s) 745, 796, 874
AvaI CYCGRG 2 cut(s) 29, 332
AvaII GGWCC 2 cut(s) 292, 340
AxyI CCTNAGG 1 cut(s) 509
BaeGI GKGCMC 1 cut(s) 265
BanI GGYRCC 2 cut(s) 207, 562
BanII GRGCYC 1 cut(s) 265
BarI GAAGNNNNNNTAC 2 cut(s) 578, 610
BbsI GAAGAC 2 cut(s) 306, 833
BbvI GCAGC 1 cut(s) 27
BccI CCATC 2 cut(s) 670, 853
BceAI ACGGC 5 cut(s) 91, 139, 143, 297, 322
BcnI CCSGG 2 cut(s) 128, 296
BcoDI GTCTC 1 cut(s) 431
BfaI CTAG 1 cut(s) 176
BfoI RGCGCY 3 cut(s) 287, 332, 566
BglI GCCNNNNNGGC 1 cut(s) 326
BisI GCNGC 5 cut(s) 16, 57, 250, 357, 557
BlsI GCNGC 5 cut(s) 17, 58, 251, 358, 558
BmcAI AGTACT 1 cut(s) 596
Bme1390I CCNGG 2 cut(s) 128, 296
Bme18I GGWCC 2 cut(s) 292, 340
BmeRI GACNNNNNGTC 1 cut(s) 453
BmeT110I CYCGRG 2 cut(s) 29, 332
BmgT120I GGNCC 7 cut(s) 125, 261, 262, 292, 340, 354, 398
BmiI GGNNCC 7 cut(s) 209, 263, 294, 564, 639, 780, 801
BmrFI CCNGG 2 cut(s) 128, 296
BmrI ACTGGG 1 cut(s) 259
BmuI ACTGGG 1 cut(s) 259
BpiI GAAGAC 2 cut(s) 306, 833
BpuMI CCSGG 2 cut(s) 128, 296
BsaHI GRCGYC 1 cut(s) 563
BsaJI CCNNGG 2 cut(s) 371, 621
BsaWI WCCGGW 2 cut(s) 239, 781
Bsc4I CCNNNNNNNGG 6 cut(s) 142, 186, 239, 259, 372, 377
Bse118I RCCGGY 1 cut(s) 559
Bse1I ACTGG 2 cut(s) 209, 265
Bse21I CCTNAGG 1 cut(s) 509
BseAI TCCGGA 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 371, 621
BseGI GGATG 4 cut(s) 10, 819, 828, 864
BseLI CCNNNNNNNGG 6 cut(s) 142, 186, 239, 259, 372, 377
BseMII CTCAG 1 cut(s) 500
BseNI ACTGG 2 cut(s) 209, 265
BseRI GAGGAG 4 cut(s) 512, 695, 734, 765
BseSI GKGCMC 1 cut(s) 265
BseXI GCAGC 1 cut(s) 27
Bsh1236I CGCG 3 cut(s) 117, 192, 359
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 6 cut(s) 126, 263, 356, 400, 431, 556
BshNI GGYRCC 2 cut(s) 207, 562
BsiEI CGRYCG 1 cut(s) 201
BsiHKCI CYCGRG 2 cut(s) 29, 332
BslI CCNNNNNNNGG 6 cut(s) 142, 186, 239, 259, 372, 377
BsmAI GTCTC 1 cut(s) 431
BsmBI CGTCTC 1 cut(s) 431
BsnI GGCC 6 cut(s) 126, 263, 356, 400, 431, 556
BsoBI CYCGRG 2 cut(s) 29, 332
Bsp120I GGGCCC 1 cut(s) 261
Bsp1286I GDGCHC 1 cut(s) 265
Bsp13I TCCGGA 1 cut(s) 239
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 2 cut(s) 7, 61
Bsp19I CCATGG 1 cut(s) 621
BspACI CCGC 7 cut(s) 57, 115, 247, 250, 357, 557, 811
BspANI GGCC 6 cut(s) 126, 263, 356, 400, 431, 556
BspCNI CTCAG 1 cut(s) 501
BspEI TCCGGA 1 cut(s) 239
BspFNI CGCG 3 cut(s) 117, 192, 359
BspLI GGNNCC 7 cut(s) 209, 263, 294, 564, 639, 780, 801
BspPI GGATC 1 cut(s) 2
BspT107I GGYRCC 2 cut(s) 207, 562
BsrFI RCCGGY 1 cut(s) 559
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 2 cut(s) 209, 265
BssAI RCCGGY 1 cut(s) 559
BssECI CCNNGG 2 cut(s) 371, 621
BssMI GATC 2 cut(s) 7, 61
BssNI GRCGYC 1 cut(s) 563
BssT1I CCWWGG 1 cut(s) 621
Bst4CI ACNGT 2 cut(s) 451, 838
Bst6I CTCTTC 6 cut(s) 113, 179, 414, 641, 662, 782
BstACI GRCGYC 1 cut(s) 563
BstAUI TGTACA 1 cut(s) 480
BstC8I GCNNGC 2 cut(s) 115, 561
BstDEI CTNAG 1 cut(s) 509
BstDSI CCRYGG 2 cut(s) 371, 621
BstF5I GGATG 4 cut(s) 10, 819, 828, 864
BstFNI CGCG 3 cut(s) 117, 192, 359
BstH2I RGCGCY 3 cut(s) 287, 332, 566
BstHHI GCGC 6 cut(s) 192, 286, 331, 361, 390, 565
BstKTI GATC 2 cut(s) 10, 64
BstMAI GTCTC 1 cut(s) 431
BstMBI GATC 2 cut(s) 7, 61
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 7 cut(s) 21, 110, 123, 283, 326, 356, 562
BstNSI RCATGY 1 cut(s) 475
BstSCI CCNGG 2 cut(s) 126, 294
BstSLI GKGCMC 1 cut(s) 265
BstUI CGCG 3 cut(s) 117, 192, 359
BstV1I GCAGC 1 cut(s) 27
BstV2I GAAGAC 2 cut(s) 306, 833
Bsu36I CCTNAGG 1 cut(s) 509
BsuRI GGCC 6 cut(s) 126, 263, 356, 400, 431, 556
BtgI CCRYGG 2 cut(s) 371, 621
BtsCI GGATG 4 cut(s) 10, 819, 828, 864
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 25, 843
Cac8I GCNNGC 2 cut(s) 115, 561
CfoI GCGC 6 cut(s) 192, 286, 331, 361, 390, 565
Cfr10I RCCGGY 1 cut(s) 559
Cfr13I GGNCC 7 cut(s) 125, 261, 262, 292, 340, 354, 398
Csp6I GTAC 6 cut(s) 26, 208, 460, 481, 595, 628
CviAII CATG 2 cut(s) 472, 622
CviQI GTAC 6 cut(s) 26, 208, 460, 481, 595, 628
DdeI CTNAG 1 cut(s) 509
DinI GGCGCC 1 cut(s) 564
DpnI GATC 2 cut(s) 9, 63
DpnII GATC 2 cut(s) 7, 61
DraIII CACNNNGTG 1 cut(s) 544
DriI GACNNNNNGTC 1 cut(s) 453
EaeI YGGCCR 1 cut(s) 429
Eam1104I CTCTTC 6 cut(s) 113, 179, 414, 641, 662, 782
Eam1105I GACNNNNNGTC 1 cut(s) 453
EarI CTCTTC 6 cut(s) 113, 179, 414, 641, 662, 782
EciI GGCGGA 1 cut(s) 826
Eco130I CCWWGG 1 cut(s) 621
Eco24I GRGCYC 1 cut(s) 265
Eco47I GGWCC 2 cut(s) 292, 340
Eco81I CCTNAGG 1 cut(s) 509
Eco88I CYCGRG 2 cut(s) 29, 332
EcoT14I CCWWGG 1 cut(s) 621
EcoT38I GRGCYC 1 cut(s) 265
EgeI GGCGCC 1 cut(s) 564
EheI GGCGCC 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 621
Esp3I CGTCTC 1 cut(s) 431
FaeI CATG 2 cut(s) 475, 625
FaiI YATR 5 cut(s) 468, 473, 479, 609, 623
FatI CATG 2 cut(s) 471, 621
FauI CCCGC 1 cut(s) 122
Fnu4HI GCNGC 5 cut(s) 16, 57, 250, 357, 557
FokI GGATG 4 cut(s) 17, 826, 835, 871
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 5 cut(s) 16, 57, 250, 357, 557
FspBI CTAG 1 cut(s) 176
GlaI GCGC 6 cut(s) 191, 285, 330, 360, 389, 564
GluI GCNGC 5 cut(s) 16, 57, 250, 357, 557
HaeII RGCGCY 3 cut(s) 287, 332, 566
HaeIII GGCC 6 cut(s) 126, 263, 356, 400, 431, 556
HhaI GCGC 6 cut(s) 192, 286, 331, 361, 390, 565
Hin1I GRCGYC 1 cut(s) 563
Hin1II CATG 2 cut(s) 475, 625
Hin6I GCGC 6 cut(s) 190, 284, 329, 359, 388, 563
HinP1I GCGC 6 cut(s) 190, 284, 329, 359, 388, 563
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 4 cut(s) 32, 51, 150, 236
HphI GGTGA 3 cut(s) 745, 796, 874
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 7 cut(s) 12, 37, 50, 367, 380, 519, 709
Hpy188III TCNNGA 3 cut(s) 240, 271, 445
Hpy8I GTNNAC 1 cut(s) 343
Hpy99I CGWCG 5 cut(s) 77, 143, 164, 170, 326
HpyAV CCTTC 7 cut(s) 481, 523, 580, 764, 769, 790, 811
HpyCH4III ACNGT 2 cut(s) 451, 838
HpyCH4IV ACGT 2 cut(s) 168, 440
HpyCH4V TGCA 1 cut(s) 220
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 110, 123, 283, 326, 356, 562
HpyF3I CTNAG 1 cut(s) 509
HpySE526I ACGT 2 cut(s) 168, 440
Hsp92I GRCGYC 1 cut(s) 563
Hsp92II CATG 2 cut(s) 475, 625
HspAI GCGC 6 cut(s) 190, 284, 329, 359, 388, 563
KasI GGCGCC 1 cut(s) 562
Kpn2I TCCGGA 1 cut(s) 239
KpnI GGTACC 1 cut(s) 211
KroI GCCGGC 1 cut(s) 559
KroNI GCCGGC 1 cut(s) 561
Kzo9I GATC 2 cut(s) 7, 61
LmnI GCTCC 3 cut(s) 589, 784, 805
Lsp1109I GCAGC 1 cut(s) 27
MaeI CTAG 1 cut(s) 176
MaeII ACGT 2 cut(s) 168, 440
MalI GATC 2 cut(s) 9, 63
MboI GATC 2 cut(s) 7, 61
MboII GAAGA 9 cut(s) 100, 196, 311, 431, 658, 679, 748, 799, 838
MhlI GDGCHC 1 cut(s) 265
MluCI AATT 2 cut(s) 98, 880
Mly113I GGCGCC 1 cut(s) 563
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 3 cut(s) 270, 390, 852
MreI CGCCGGCG 1 cut(s) 559
MroI TCCGGA 1 cut(s) 239
MroNI GCCGGC 1 cut(s) 559
MroXI GAANNNNTTC 1 cut(s) 42
MslI CAYNNNNRTG 1 cut(s) 476
MspR9I CCNGG 2 cut(s) 128, 296
MvnI CGCG 3 cut(s) 117, 192, 359
MwoI GCNNNNNNNGC 7 cut(s) 21, 110, 123, 283, 326, 356, 562
NaeI GCCGGC 1 cut(s) 561
NarI GGCGCC 1 cut(s) 563
NciI CCSGG 2 cut(s) 128, 296
NcoI CCATGG 1 cut(s) 621
NdeII GATC 2 cut(s) 7, 61
NgoMIV GCCGGC 1 cut(s) 559
NlaIII CATG 2 cut(s) 475, 625
NlaIV GGNNCC 7 cut(s) 209, 263, 294, 564, 639, 780, 801
NmeAIII GCCGAG 1 cut(s) 205
NspI RCATGY 1 cut(s) 475
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 455
PdiI GCCGGC 1 cut(s) 561
PdmI GAANNNNTTC 1 cut(s) 42
PfeI GAWTC 3 cut(s) 51, 150, 236
PkrI GCNGC 5 cut(s) 17, 58, 251, 358, 558
PleI GAGTC 1 cut(s) 40
PluTI GGCGCC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 40
PspN4I GGNNCC 7 cut(s) 209, 263, 294, 564, 639, 780, 801
PspOMI GGGCCC 1 cut(s) 261
PspPI GGNCC 7 cut(s) 125, 261, 262, 292, 340, 354, 398
RsaI GTAC 6 cut(s) 27, 209, 461, 482, 596, 629
RsaNI GTAC 6 cut(s) 26, 208, 460, 481, 595, 628
RseI CAYNNNNRTG 1 cut(s) 476
SatI GCNGC 5 cut(s) 16, 57, 250, 357, 557
Sau3AI GATC 2 cut(s) 7, 61
Sau96I GGNCC 7 cut(s) 125, 261, 262, 292, 340, 354, 398
ScaI AGTACT 1 cut(s) 596
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 2 cut(s) 128, 296
SduI GDGCHC 1 cut(s) 265
SetI ASST 7 cut(s) 45, 171, 279, 443, 492, 515, 726
SfoI GGCGCC 1 cut(s) 564
SgrAI CRCCGGYG 1 cut(s) 559
SinI GGWCC 2 cut(s) 292, 340
SmiMI CAYNNNNRTG 1 cut(s) 476
Sse9I AATT 2 cut(s) 98, 880
SsiI CCGC 7 cut(s) 57, 115, 247, 250, 357, 557, 811
SspDI GGCGCC 1 cut(s) 562
SspMI CTAG 1 cut(s) 176
StyD4I CCNGG 2 cut(s) 126, 294
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 2 cut(s) 451, 838
TaiI ACGT 2 cut(s) 171, 443
TaqI TCGA 2 cut(s) 270, 764
TaqII GACCGA 1 cut(s) 215
TasI AATT 2 cut(s) 98, 880
TatI WGTACW 3 cut(s) 480, 594, 627
TauI GCSGC 4 cut(s) 59, 252, 359, 559
TfiI GAWTC 3 cut(s) 51, 150, 236
TscAI CASTG 2 cut(s) 25, 843
TseI GCWGC 1 cut(s) 15
TspDTI ATGAA 3 cut(s) 809, 830, 839
TspGWI ACGGA 1 cut(s) 447
TspRI CASTG 2 cut(s) 25, 843
VpaK11BI GGWCC 2 cut(s) 292, 340
XapI RAATTY 1 cut(s) 98
XceI RCATGY 1 cut(s) 475
XmnI GAANNNNTTC 1 cut(s) 42
XspI CTAG 1 cut(s) 176
ZrmI AGTACT 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.