Rw2G017450

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017224
Physical Location & Seq
Forward (+)
21534866 .. 21535842
977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G017450.1

Sequence Viewer

Length: 837 bp
ATGGATAATCCGAGCAGCAGTGGCGTACCCGAGTCCGAAAAGCTTCTCGGAATCGCCGCCGATCTCCTCTGCGACGGCAACTTCGCTTCTTGCCGGAAATTCGCTCTCTTCTCCCGTGACTACGACCCGGAAAACCCCGTCGTGGAAAGAATCCTCGCCGTCGCCGACGTTCTCCTCGCCGAAAAGAGGCGCGACCCGACCGACTGGTACCCCATTTTGCAACTGACCCGACCCGACTCCGCAAACCGCCGCCTCGTTCGGACCCAGTTCGAGAAGCTCGCGGCGCTGTTGAACCCGGAAGACAACGGCTTCCCGTTTTCCGACGAGGCGCTCGGGTTGGTCCACAAGGCGTGGGCCGCGCTAGGGTTTGGCGGAGAGACGTTCTGGACGGTGTGTCCGTACTGCTATGGCATGTTTATGTACAAGAAGGTGTTTGAGGAGTGTTGCCTGAGGTGTCAGATTTGCCGGAAGGCGTTTCACGGCGTGGCGATTAGGCCGCCGGCGCCGGAGATTTTGGTGGAGGGGAAGGAGCAGTACTATTTCACTTATGGGTGTTTTCCTATGGAGTACAATGAGGAGCAAAGAAGAGTAATGAGGACACAAAGAAGAGGCAGATGGAGGAGGTGTTTGTGTTGTGGAATTTCTGATGATGAGGAGGAGGTGGTGGTGAAGAATGTGGGTGGAGGAGGGAAGTTTTCGAGTGAAGGGAAGGCTCCGGTGAAGAGAATGAAGGCTCCGGCGAGGAGGATGAAGGGGATGAAGACGAAAACAGTGGCGAGTAGGAGGTTTTTTGAAGGTGAAGATGATGTCTTTGTTGGTCTTGGGGATAGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

278

Amino Acids

31.52

Weight (kDa)

8.21

Isoelectric Point (pI)

62.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017224)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19770
malus_domestica MD15G1313800.v1.1
prunus_persica Prupe.6G198600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0111341
rosa_laevigata RLG00000017887
rosa_multiflora Rmu_co8456405.1_g000001
rosa_roxburghii Rroxscaffold_2G00132480
rosa_rugosa Rorug02G0172800
rosa_samantha Rh2AG226000 Rh2BG237800 Rh2CG229000 Rh2DG233500
rosa_wichuraiana Rw2G017450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 207
AccB1I GGYRCC 2 cut(s) 207, 502
AccII CGCG 3 cut(s) 192, 281, 359
AciI CCGC 8 cut(s) 57, 240, 247, 250, 281, 357, 372, 497
AcsI RAATTY 2 cut(s) 98, 639
AcyI GRCGYC 1 cut(s) 503
AdeI CACNNNGTG 1 cut(s) 484
AfaI GTAC 6 cut(s) 27, 209, 401, 422, 536, 569
AfiI CCNNNNNNNGG 3 cut(s) 142, 186, 363
AgsI TTSAA 2 cut(s) 292, 794
AhdI GACNNNNNGTC 1 cut(s) 393
AluBI AGCT 2 cut(s) 43, 277
AluI AGCT 2 cut(s) 43, 277
Alw26I GTCTC 1 cut(s) 371
Ama87I CYCGRG 2 cut(s) 29, 332
AoxI GGCC 2 cut(s) 354, 494
ApeKI GCWGC 1 cut(s) 15
ApoI RAATTY 2 cut(s) 98, 639
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
Asp700I GAANNNNTTC 1 cut(s) 42
Asp718I GGTACC 1 cut(s) 207
AspLEI GCGC 5 cut(s) 192, 286, 331, 361, 505
AspS9I GGNCC 3 cut(s) 261, 340, 354
AsuC2I CCSGG 2 cut(s) 128, 296
AsuHPI GGTGA 3 cut(s) 679, 730, 809
AvaI CYCGRG 2 cut(s) 29, 332
AvaII GGWCC 2 cut(s) 261, 340
AxyI CCTNAGG 1 cut(s) 449
BanI GGYRCC 2 cut(s) 207, 502
BarI GAAGNNNNNNTAC 2 cut(s) 518, 550
BbsI GAAGAC 2 cut(s) 306, 767
BbvI GCAGC 1 cut(s) 27
BccI CCATC 1 cut(s) 609
BceAI ACGGC 4 cut(s) 91, 143, 322, 496
BcnI CCSGG 2 cut(s) 128, 296
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 1 cut(s) 362
BfoI RGCGCY 3 cut(s) 287, 332, 506
BisI GCNGC 6 cut(s) 16, 57, 250, 282, 357, 497
BlsI GCNGC 6 cut(s) 17, 58, 251, 283, 358, 498
BmcAI AGTACT 1 cut(s) 536
Bme1390I CCNGG 2 cut(s) 128, 296
Bme18I GGWCC 2 cut(s) 261, 340
BmeRI GACNNNNNGTC 1 cut(s) 393
BmeT110I CYCGRG 2 cut(s) 29, 332
BmgT120I GGNCC 3 cut(s) 261, 340, 354
BmiI GGNNCC 5 cut(s) 209, 263, 504, 714, 735
BmrFI CCNGG 2 cut(s) 128, 296
BmrI ACTGGG 1 cut(s) 259
BmuI ACTGGG 1 cut(s) 259
BpiI GAAGAC 2 cut(s) 306, 767
BpuMI CCSGG 2 cut(s) 128, 296
BsaHI GRCGYC 1 cut(s) 503
BsaWI WCCGGW 1 cut(s) 715
BsaXI ACNNNNNCTCC 2 cut(s) 610, 640
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 186, 363
Bse118I RCCGGY 1 cut(s) 499
Bse1I ACTGG 2 cut(s) 209, 265
Bse21I CCTNAGG 1 cut(s) 449
BseGI GGATG 2 cut(s) 753, 762
BseLI CCNNNNNNNGG 3 cut(s) 142, 186, 363
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 2 cut(s) 209, 265
BseRI GAGGAG 9 cut(s) 56, 164, 452, 590, 634, 668, 671, 699, 757
BseXI GCAGC 1 cut(s) 27
Bsh1236I CGCG 3 cut(s) 192, 281, 359
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 2 cut(s) 356, 496
BshNI GGYRCC 2 cut(s) 207, 502
BsiEI CGRYCG 1 cut(s) 201
BsiHKCI CYCGRG 2 cut(s) 29, 332
BsiSI CCGG 8 cut(s) 94, 128, 296, 466, 500, 506, 716, 737
BslI CCNNNNNNNGG 3 cut(s) 142, 186, 363
BsmAI GTCTC 1 cut(s) 371
BsmBI CGTCTC 1 cut(s) 371
BsnI GGCC 2 cut(s) 356, 496
BsoBI CYCGRG 2 cut(s) 29, 332
Bsp1407I TGTACA 1 cut(s) 420
Bsp143I GATC 1 cut(s) 61
BspACI CCGC 8 cut(s) 57, 240, 247, 250, 281, 357, 372, 497
BspANI GGCC 2 cut(s) 356, 496
BspCNI CTCAG 1 cut(s) 441
BspFNI CGCG 3 cut(s) 192, 281, 359
BspLI GGNNCC 5 cut(s) 209, 263, 504, 714, 735
BspT107I GGYRCC 2 cut(s) 207, 502
BsrFI RCCGGY 1 cut(s) 499
BsrGI TGTACA 1 cut(s) 420
BsrI ACTGG 2 cut(s) 209, 265
BssAI RCCGGY 1 cut(s) 499
BssMI GATC 1 cut(s) 61
BssNI GRCGYC 1 cut(s) 503
Bst4CI ACNGT 2 cut(s) 391, 772
Bst6I CTCTTC 4 cut(s) 113, 580, 601, 716
BstACI GRCGYC 1 cut(s) 503
BstAUI TGTACA 1 cut(s) 420
BstC8I GCNNGC 2 cut(s) 279, 501
BstDEI CTNAG 1 cut(s) 449
BstF5I GGATG 2 cut(s) 753, 762
BstFNI CGCG 3 cut(s) 192, 281, 359
BstH2I RGCGCY 3 cut(s) 287, 332, 506
BstHHI GCGC 5 cut(s) 192, 286, 331, 361, 505
BstKTI GATC 1 cut(s) 64
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 1 cut(s) 61
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 4 cut(s) 21, 283, 356, 502
BstNSI RCATGY 1 cut(s) 415
BstSCI CCNGG 2 cut(s) 126, 294
BstUI CGCG 3 cut(s) 192, 281, 359
BstV1I GCAGC 1 cut(s) 27
BstV2I GAAGAC 2 cut(s) 306, 767
Bsu36I CCTNAGG 1 cut(s) 449
BsuRI GGCC 2 cut(s) 356, 496
BtsCI GGATG 2 cut(s) 753, 762
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 25, 777
Cac8I GCNNGC 2 cut(s) 279, 501
CfoI GCGC 5 cut(s) 192, 286, 331, 361, 505
Cfr10I RCCGGY 1 cut(s) 499
Cfr13I GGNCC 3 cut(s) 261, 340, 354
Csp6I GTAC 6 cut(s) 26, 208, 400, 421, 535, 568
CviAII CATG 2 cut(s) 412, 834
CviJI RGCY 7 cut(s) 43, 277, 309, 356, 496, 713, 734
CviKI_1 RGCY 7 cut(s) 43, 277, 309, 356, 496, 713, 734
CviQI GTAC 6 cut(s) 26, 208, 400, 421, 535, 568
DdeI CTNAG 1 cut(s) 449
DinI GGCGCC 1 cut(s) 504
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
DraIII CACNNNGTG 1 cut(s) 484
DriI GACNNNNNGTC 1 cut(s) 393
Eam1104I CTCTTC 4 cut(s) 113, 580, 601, 716
Eam1105I GACNNNNNGTC 1 cut(s) 393
EarI CTCTTC 4 cut(s) 113, 580, 601, 716
EciI GGCGGA 1 cut(s) 387
Eco47I GGWCC 2 cut(s) 261, 340
Eco81I CCTNAGG 1 cut(s) 449
Eco88I CYCGRG 2 cut(s) 29, 332
EgeI GGCGCC 1 cut(s) 504
EheI GGCGCC 1 cut(s) 504
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 2 cut(s) 415, 837
FaiI YATR 6 cut(s) 408, 413, 419, 549, 563, 835
FatI CATG 2 cut(s) 411, 833
Fnu4HI GCNGC 6 cut(s) 16, 57, 250, 282, 357, 497
FokI GGATG 2 cut(s) 760, 769
Fsp4HI GCNGC 6 cut(s) 16, 57, 250, 282, 357, 497
FspBI CTAG 1 cut(s) 362
GlaI GCGC 5 cut(s) 191, 285, 330, 360, 504
GluI GCNGC 6 cut(s) 16, 57, 250, 282, 357, 497
HaeII RGCGCY 3 cut(s) 287, 332, 506
HaeIII GGCC 2 cut(s) 356, 496
HapII CCGG 8 cut(s) 94, 128, 296, 466, 500, 506, 716, 737
HhaI GCGC 5 cut(s) 192, 286, 331, 361, 505
Hin1I GRCGYC 1 cut(s) 503
Hin1II CATG 2 cut(s) 415, 837
Hin6I GCGC 5 cut(s) 190, 284, 329, 359, 503
HinP1I GCGC 5 cut(s) 190, 284, 329, 359, 503
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 4 cut(s) 32, 51, 150, 236
HpaII CCGG 8 cut(s) 94, 128, 296, 466, 500, 506, 716, 737
HphI GGTGA 3 cut(s) 679, 730, 809
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 7 cut(s) 12, 37, 50, 261, 322, 459, 646
Hpy188III TCNNGA 2 cut(s) 271, 385
Hpy8I GTNNAC 1 cut(s) 343
Hpy99I CGWCG 5 cut(s) 77, 143, 164, 170, 326
HpyAV CCTTC 8 cut(s) 421, 463, 520, 698, 703, 724, 745, 788
HpyCH4III ACNGT 2 cut(s) 391, 772
HpyCH4IV ACGT 2 cut(s) 168, 380
HpyCH4V TGCA 1 cut(s) 220
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 283, 356, 502
HpyF3I CTNAG 1 cut(s) 449
HpySE526I ACGT 2 cut(s) 168, 380
Hsp92I GRCGYC 1 cut(s) 503
Hsp92II CATG 2 cut(s) 415, 837
HspAI GCGC 5 cut(s) 190, 284, 329, 359, 503
KasI GGCGCC 1 cut(s) 502
KpnI GGTACC 1 cut(s) 211
KroI GCCGGC 1 cut(s) 499
KroNI GCCGGC 1 cut(s) 501
Kzo9I GATC 1 cut(s) 61
LmnI GCTCC 4 cut(s) 529, 577, 718, 739
Lsp1109I GCAGC 1 cut(s) 27
MaeI CTAG 1 cut(s) 362
MaeII ACGT 2 cut(s) 168, 380
MaeIII GTNAC 1 cut(s) 116
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MboII GAAGA 8 cut(s) 100, 311, 597, 618, 682, 733, 772, 812
MluCI AATT 2 cut(s) 98, 639
Mly113I GGCGCC 1 cut(s) 503
MlyI GAGTC 2 cut(s) 41, 230
MmeI TCCRAC 1 cut(s) 345
MreI CGCCGGCG 1 cut(s) 499
MroNI GCCGGC 1 cut(s) 499
MroXI GAANNNNTTC 1 cut(s) 42
MslI CAYNNNNRTG 1 cut(s) 416
MspI CCGG 8 cut(s) 94, 128, 296, 466, 500, 506, 716, 737
MspR9I CCNGG 2 cut(s) 128, 296
MvnI CGCG 3 cut(s) 192, 281, 359
MwoI GCNNNNNNNGC 4 cut(s) 21, 283, 356, 502
NaeI GCCGGC 1 cut(s) 501
NarI GGCGCC 1 cut(s) 503
NciI CCSGG 2 cut(s) 128, 296
NdeII GATC 1 cut(s) 61
NgoMIV GCCGGC 1 cut(s) 499
NlaIII CATG 2 cut(s) 415, 837
NlaIV GGNNCC 5 cut(s) 209, 263, 504, 714, 735
NmuCI GTSAC 1 cut(s) 116
NspI RCATGY 1 cut(s) 415
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 395
PdiI GCCGGC 1 cut(s) 501
PdmI GAANNNNTTC 1 cut(s) 42
PfeI GAWTC 2 cut(s) 51, 150
PkrI GCNGC 6 cut(s) 17, 58, 251, 283, 358, 498
PleI GAGTC 2 cut(s) 40, 230
PluTI GGCGCC 1 cut(s) 506
PpsI GAGTC 2 cut(s) 40, 230
PspN4I GGNNCC 5 cut(s) 209, 263, 504, 714, 735
PspPI GGNCC 3 cut(s) 261, 340, 354
RsaI GTAC 6 cut(s) 27, 209, 401, 422, 536, 569
RsaNI GTAC 6 cut(s) 26, 208, 400, 421, 535, 568
RseI CAYNNNNRTG 1 cut(s) 416
SatI GCNGC 6 cut(s) 16, 57, 250, 282, 357, 497
Sau3AI GATC 1 cut(s) 61
Sau96I GGNCC 3 cut(s) 261, 340, 354
ScaI AGTACT 1 cut(s) 536
SchI GAGTC 2 cut(s) 41, 230
ScrFI CCNGG 2 cut(s) 128, 296
SfoI GGCGCC 1 cut(s) 504
SgrAI CRCCGGYG 1 cut(s) 499
SinI GGWCC 2 cut(s) 261, 340
SmiMI CAYNNNNRTG 1 cut(s) 416
Sse9I AATT 2 cut(s) 98, 639
SsiI CCGC 8 cut(s) 57, 240, 247, 250, 281, 357, 372, 497
SspDI GGCGCC 1 cut(s) 502
SspMI CTAG 1 cut(s) 362
StyD4I CCNGG 2 cut(s) 126, 294
TaaI ACNGT 2 cut(s) 391, 772
TaiI ACGT 2 cut(s) 171, 383
TaqI TCGA 2 cut(s) 270, 698
TaqII GACCGA 1 cut(s) 215
TasI AATT 2 cut(s) 98, 639
TatI WGTACW 3 cut(s) 420, 534, 567
TauI GCSGC 5 cut(s) 59, 252, 284, 359, 499
TfiI GAWTC 2 cut(s) 51, 150
TscAI CASTG 2 cut(s) 25, 777
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 1 cut(s) 15
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 743, 764, 773
TspGWI ACGGA 1 cut(s) 387
TspRI CASTG 2 cut(s) 25, 777
VpaK11BI GGWCC 2 cut(s) 261, 340
XapI RAATTY 2 cut(s) 98, 639
XceI RCATGY 1 cut(s) 415
XmnI GAANNNNTTC 1 cut(s) 42
XspI CTAG 1 cut(s) 362
ZrmI AGTACT 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.