Rroxscaffold_2G00139900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017221
Physical Location & Seq
Forward (+)
78177741 .. 78179717
1977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139900.1

Sequence Viewer

Length: 1977 bp
ATGTACTTCCCCCTCAAGCATCCGACCGTTGTTTCCCGCCTCTTTTCCTCTCAGTCTCTGCAAACCAATGTCACCCACAATTTCAAGACCCAAATTCACGAAACCCTGCACCGCCTGGTAGAGCAATGCTCCTCCATGTCACACCTCAAACAACTCCACGCCCAGATCATCCTCCACCGCCTCACCAACCAAAACCTCACCCTCGGAAAGCTCATTTCCTTCTGCTCCGTCTCCGACGCCGGAGACCTCCGTTACGCTCACCTTGTGTTTGACCAAGTACATCAACCCAATAAATTTATGTACAATAGTCTAATCAGGGGCTACTCAAATAGCAATGACTCATTCAAGGCTATGTCTTTGTATTACCGAATGATAAATTCCGGGCTTGCTCCGAACGAATTTACATTGCCGTTTGTTCTTAAGGTTTGCGCCGGAACAATGGCGTATTGGGAAGGCGTGGTGGTTCATGGTCAGGCTGTTAAGCTTGGAATTGGGTGTCAAGTTTGCGTGCAAAATGGGCTTATTAATGTGTATAGTGTTTGTGGGTTGATACATTGTGCCCGGAATGTGTTTGATGAAATGTCTGAGAGAAGTTTGGTGTCGTGGAATTCGATGATAGGTGGGTATGCTGGAGTTGGTTCTTGGAAGGAAGCTTTCGGGTTGTTCAGGGAGATTAGAGATTTCGGCATTGAGCCTGATAAGTTCACATTGGTTAATTTACTTTCTGTTTGTTCACAGGCTTGTGATTTGGAGTTCGGGAGATATCTGCATCACTATATTGTGGTTAGTGGGATGGTGGTTGATCAGATTTTGAGAAATGCTCTGCTGGATATGTATGGTAAGTGTGGGCATTTGCCTTCGGCTCAGATGGTTTTCGACCAAATGAGTTACAAAAATGTGGTTTCTTGGACGTCGATGGTTAGGGCGTATGCTAGACATGGGCGCATTGAAGTTGCGCAGAAGTTTTTTGATCAGATGCCACTGAAAAATGTGGTTTCTTGGAATTCACTAATTTCGTGTTATGTGCGAGAAGGCCAATGCAGGGAAGCTCTGGATCTCTTCCAGAAAATGCTCAATTCTGGTGAGGTGCCTGATGAGGCTACCTTGGTTTTCGCTCTCTCAGCCTGTAGTCAAATTGGTGATTTGGTCATTGGAAAGGAAACCCACAATTACATTGGCAACAGCAATATAGCACTCACTGTAACTCTTTCTAATTCCCTTGTAGACATGTATGCGAAATGTGGTGCTGTTGGAACTGCTATGGATCTTTTTAGTCAGATACCAAAAAAGAATGTAGTATCATGGAACATTGTTATTGGTGCCCTTGCATTGCACGGTTATGGATCAGAAGCAATTAGGATATTTGAGCAGATGCAAGAAGGTGGAATCTGGCCTGATGAGATCACCTTCATCGGATTGCTTTCTGCTTGTAGTCACAGTGGTCTTCTAGACTTGGGGAGATATTACTTTGACAGAATGAAGTCTATTTACAGAATCTCTCCTGAAATTGAGCACTATGCTTGCATGGTTGATCTGCTGGGACGACGTGGGTGCTTAGAAGGAGCAATTACACTGATGAGAGGAATGCCAATGAAGCCTGATATTGTTGTTTGGGGTGCCATGCTTGGTGCTTGTCGCATCCATGGCAACGTAGATTTATCCAAGCTAATTCTAAAACAGCTGTTGGAGTTGGAGCCACATGGTTCTGGGCTTTACGTGCTTCTTGCGAACATATTTGGTGAAGCTCACAGATGGGAAGATGTCAAGAATATCAGGAAACTAATAAAAGACGGTGGAGGCATAAAGTGTAGAGCTGTGAGCTCCATTGAAATTGATGGTTGTGTTTATGAATTTATGGTTGATGACAATAGACATGCAATTTCAATCAGTATATACTCCATGCTCAATCAATTGACAGATCATCTGAAGTCTCTTGGGTATAATCCTACTGCAATGTGTGATTTAGAGGAACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

658

Amino Acids

73.66

Weight (kDa)

6.66

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 96 - 144 1.4e-07 PPR repeat family
PPR PF01535 99 - 129 5.3e-06 PPR repeat
PPR_2 PF13041 198 - 244 2.2e-09 PPR repeat family
PPR PF01535 200 - 230 7.2e-07 PPR repeat
PPR PF01535 301 - 330 2.1e-06 PPR repeat
PPR_2 PF13041 329 - 376 4.9e-11 PPR repeat family
PPR PF01535 332 - 361 1.7e-09 PPR repeat
PPR_2 PF13041 430 - 478 2.5e-10 PPR repeat family
PPR PF01535 433 - 463 7.8e-06 PPR repeat
E_motif PF20431 548 - 610 1.1e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017221)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14410
malus_domestica MD02G1157600.v1.1
prunus_persica Prupe.7G146100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0103131
rosa_laevigata RLG00000017212
rosa_multiflora Rmu_sc0000388.1_g000006
rosa_roxburghii Rroxscaffold_2G00139900
rosa_rugosa Rorug02G0112000
rosa_samantha Rh2AG160800 Rh2BG167500 Rh2CG167800 Rh2DG166400
rosa_wichuraiana Rw2G012590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 914
Acc16I TGCGCA 1 cut(s) 957
AccB1I GGYRCC 3 cut(s) 1087, 1319, 1616
AccI GTMKAC 1 cut(s) 1224
AciI CCGC 3 cut(s) 37, 112, 178
AclWI GGATC 3 cut(s) 1062, 1272, 1351
AcsI RAATTY 7 cut(s) 93, 293, 376, 398, 607, 1003, 1850
AcuI CTGAAG 1 cut(s) 1946
AcyI GRCGYC 2 cut(s) 237, 911
AdeI CACNNNGTG 1 cut(s) 265
AfaI GTAC 3 cut(s) 5, 279, 302
AfiI CCNNNNNNNGG 1 cut(s) 1042
AflII CTTAAG 1 cut(s) 419
AflIII ACRYGT 1 cut(s) 1227
AgsI TTSAA 5 cut(s) 85, 346, 950, 1829, 1884
AjiI CACGTC 1 cut(s) 1547
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 9 cut(s) 211, 484, 653, 1049, 1666, 1681, 1745, 1814, 1821
AluI AGCT 9 cut(s) 211, 484, 653, 1049, 1666, 1681, 1745, 1814, 1821
Alw21I GWGCWC 2 cut(s) 1515, 1823
Alw26I GTCTC 4 cut(s) 60, 235, 237, 1935
AlwI GGATC 3 cut(s) 1062, 1272, 1351
AlwNI CAGNNNCTG 1 cut(s) 58
AoxI GGCC 2 cut(s) 1033, 1391
ApoI RAATTY 7 cut(s) 93, 293, 376, 398, 607, 1003, 1850
AseI ATTAAT 1 cut(s) 525
AspLEI GCGC 3 cut(s) 431, 945, 958
AsuC2I CCSGG 2 cut(s) 382, 562
AsuHPI GGTGA 8 cut(s) 64, 175, 190, 251, 1094, 1151, 1396, 1751
BaeGI GKGCMC 2 cut(s) 562, 1324
BanI GGYRCC 3 cut(s) 1087, 1319, 1616
BanII GRGCYC 1 cut(s) 1823
BarI GAAGNNNNNNTAC 2 cut(s) 1472, 1504
BbsI GAAGAC 1 cut(s) 1436
Bbv12I GWGCWC 2 cut(s) 1515, 1823
BccI CCATC 5 cut(s) 787, 862, 910, 1746, 1829
BceAI ACGGC 1 cut(s) 394
BcgI CGANNNNNNTGC 2 cut(s) 1533, 1567
BciT130I CCWGG 1 cut(s) 116
BclI TGATCA 2 cut(s) 802, 970
BcnI CCSGG 2 cut(s) 382, 562
BcoDI GTCTC 4 cut(s) 60, 235, 237, 1935
BfaI CTAG 2 cut(s) 933, 1448
BfmI CTRYAG 2 cut(s) 1126, 1973
BfrI CTTAAG 1 cut(s) 419
Bme1390I CCNGG 3 cut(s) 116, 382, 562
BmgBI CACGTC 1 cut(s) 1547
BmiI GGNNCC 4 cut(s) 1089, 1321, 1618, 1695
BmrFI CCNGG 3 cut(s) 116, 382, 562
BmsI GCATC 5 cut(s) 28, 778, 966, 1362, 1647
BpiI GAAGAC 1 cut(s) 1436
BplI GAGNNNNNCTC 4 cut(s) 113, 145, 805, 837
BpmI CTGGAG 1 cut(s) 651
BpuMI CCSGG 2 cut(s) 382, 562
BsaAI YACGTR 1 cut(s) 1717
BsaHI GRCGYC 2 cut(s) 237, 911
BsaI GGTCTC 1 cut(s) 237
BsaJI CCNNGG 3 cut(s) 202, 1104, 1642
Bsc4I CCNNNNNNNGG 1 cut(s) 1042
Bse3DI GCAATG 5 cut(s) 131, 340, 404, 1328, 1959
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 3 cut(s) 202, 1104, 1642
BseGI GGATG 4 cut(s) 19, 168, 798, 1638
BseLI CCNNNNNNNGG 1 cut(s) 1042
BseMI GCAATG 5 cut(s) 131, 340, 404, 1328, 1959
BseMII CTCAG 4 cut(s) 65, 576, 878, 1134
BseRI GAGGAG 1 cut(s) 121
BseSI GKGCMC 2 cut(s) 562, 1324
BseYI CCCAGC 1 cut(s) 1537
BsgI GTGCAG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 2 cut(s) 1035, 1393
BshNI GGYRCC 3 cut(s) 1087, 1319, 1616
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 2 cut(s) 1515, 1823
BsiSI CCGG 4 cut(s) 240, 381, 432, 562
BslFI GGGAC 1 cut(s) 1554
BslI CCNNNNNNNGG 1 cut(s) 1042
BsmAI GTCTC 4 cut(s) 60, 235, 237, 1935
BsmBI CGTCTC 1 cut(s) 235
BsmFI GGGAC 1 cut(s) 1554
BsmI GAATGC 1 cut(s) 1590
BsnI GGCC 2 cut(s) 1035, 1393
Bso31I GGTCTC 1 cut(s) 237
Bsp1286I GDGCHC 4 cut(s) 562, 1324, 1515, 1823
Bsp1407I TGTACA 1 cut(s) 300
Bsp143I GATC 9 cut(s) 165, 802, 970, 1054, 1264, 1343, 1401, 1531, 1918
Bsp19I CCATGG 1 cut(s) 1642
BspACI CCGC 3 cut(s) 37, 112, 178
BspANI GGCC 2 cut(s) 1035, 1393
BspCNI CTCAG 4 cut(s) 64, 577, 877, 1133
BspLI GGNNCC 4 cut(s) 1089, 1321, 1618, 1695
BspPI GGATC 3 cut(s) 1062, 1272, 1351
BspT107I GGYRCC 3 cut(s) 1087, 1319, 1616
BspTI CTTAAG 1 cut(s) 419
BspTNI GGTCTC 1 cut(s) 237
BsrDI GCAATG 5 cut(s) 131, 340, 404, 1328, 1959
BsrGI TGTACA 1 cut(s) 300
BssECI CCNNGG 3 cut(s) 202, 1104, 1642
BssMI GATC 9 cut(s) 165, 802, 970, 1054, 1264, 1343, 1401, 1531, 1918
BssNI GRCGYC 2 cut(s) 237, 911
BssT1I CCWWGG 2 cut(s) 1104, 1642
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 6 cut(s) 28, 1201, 1337, 1439, 1793, 1974
Bst6I CTCTTC 1 cut(s) 1064
BstACI GRCGYC 2 cut(s) 237, 911
BstAFI CTTAAG 1 cut(s) 419
BstAUI TGTACA 1 cut(s) 300
BstBAI YACGTR 1 cut(s) 1717
BstC8I GCNNGC 3 cut(s) 387, 509, 1522
BstDEI CTNAG 5 cut(s) 51, 585, 864, 1120, 1555
BstDSI CCRYGG 1 cut(s) 1642
BstF5I GGATG 4 cut(s) 19, 168, 798, 1638
BstHHI GCGC 3 cut(s) 431, 945, 958
BstKTI GATC 9 cut(s) 168, 805, 973, 1057, 1267, 1346, 1404, 1534, 1921
BstMAI GTCTC 4 cut(s) 60, 235, 237, 1935
BstMBI GATC 9 cut(s) 165, 802, 970, 1054, 1264, 1343, 1401, 1531, 1918
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 5 cut(s) 517, 1121, 1594, 1644, 1717
BstNI CCWGG 1 cut(s) 116
BstNSI RCATGY 2 cut(s) 1231, 1877
BstSCI CCNGG 3 cut(s) 114, 380, 560
BstSFI CTRYAG 2 cut(s) 1126, 1973
BstSLI GKGCMC 2 cut(s) 562, 1324
BstV2I GAAGAC 1 cut(s) 1436
BstX2I RGATCY 2 cut(s) 1054, 1264
BstYI RGATCY 2 cut(s) 1054, 1264
BsuRI GGCC 2 cut(s) 1035, 1393
BtgI CCRYGG 1 cut(s) 1642
BtrI CACGTC 1 cut(s) 1547
BtsCI GGATG 4 cut(s) 19, 168, 798, 1638
BtsIMutI CAGTG 4 cut(s) 980, 1197, 1444, 1571
Cac8I GCNNGC 3 cut(s) 387, 509, 1522
CaiI CAGNNNCTG 1 cut(s) 58
CfoI GCGC 3 cut(s) 431, 945, 958
CseI GACGC 1 cut(s) 245
Csp6I GTAC 3 cut(s) 4, 278, 301
CviQI GTAC 3 cut(s) 4, 278, 301
DdeI CTNAG 5 cut(s) 51, 585, 864, 1120, 1555
DpnI GATC 9 cut(s) 167, 804, 972, 1056, 1266, 1345, 1403, 1533, 1920
DpnII GATC 9 cut(s) 165, 802, 970, 1054, 1264, 1343, 1401, 1531, 1918
DraIII CACNNNGTG 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 1064
EarI CTCTTC 1 cut(s) 1064
Ecl136II GAGCTC 1 cut(s) 1821
Eco130I CCWWGG 2 cut(s) 1104, 1642
Eco24I GRGCYC 1 cut(s) 1823
Eco31I GGTCTC 1 cut(s) 237
Eco32I GATATC 1 cut(s) 764
Eco53kI GAGCTC 1 cut(s) 1821
Eco57I CTGAAG 1 cut(s) 1946
EcoICRI GAGCTC 1 cut(s) 1821
EcoRI GAATTC 2 cut(s) 607, 1003
EcoRII CCWGG 1 cut(s) 114
EcoRV GATATC 1 cut(s) 764
EcoT14I CCWWGG 2 cut(s) 1104, 1642
EcoT38I GRGCYC 1 cut(s) 1823
ErhI CCWWGG 2 cut(s) 1104, 1642
Esp3I CGTCTC 1 cut(s) 235
FaqI GGGAC 1 cut(s) 1554
FauI CCCGC 1 cut(s) 44
FbaI TGATCA 2 cut(s) 802, 970
FblI GTMKAC 1 cut(s) 1224
FokI GGATG 4 cut(s) 6, 155, 805, 1625
FriOI GRGCYC 1 cut(s) 1823
FspBI CTAG 2 cut(s) 933, 1448
FspI TGCGCA 1 cut(s) 957
GlaI GCGC 3 cut(s) 430, 944, 957
GsaI CCCAGC 1 cut(s) 1541
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 2 cut(s) 1035, 1393
HapII CCGG 4 cut(s) 240, 381, 432, 562
HgaI GACGC 1 cut(s) 245
HhaI GCGC 3 cut(s) 431, 945, 958
Hin1I GRCGYC 2 cut(s) 237, 911
Hin6I GCGC 3 cut(s) 429, 943, 956
HinP1I GCGC 3 cut(s) 429, 943, 956
HindIII AAGCTT 2 cut(s) 482, 651
HinfI GANTC 3 cut(s) 338, 1386, 1494
HpaII CCGG 4 cut(s) 240, 381, 432, 562
HphI GGTGA 8 cut(s) 64, 175, 190, 251, 1094, 1151, 1396, 1751
Hpy166II GTNNAC 3 cut(s) 705, 734, 1225
Hpy188III TCNNGA 9 cut(s) 85, 98, 757, 1052, 1063, 1448, 1502, 1765, 1774
Hpy8I GTNNAC 3 cut(s) 705, 734, 1225
Hpy99I CGWCG 3 cut(s) 239, 916, 1548
HpyAV CCTTC 8 cut(s) 229, 446, 640, 867, 1025, 1373, 1417, 1553
HpyCH4III ACNGT 6 cut(s) 28, 1201, 1337, 1439, 1793, 1974
HpyCH4IV ACGT 4 cut(s) 911, 1546, 1650, 1716
HpyF10VI GCNNNNNNNGC 5 cut(s) 517, 1121, 1594, 1644, 1717
HpyF3I CTNAG 5 cut(s) 51, 585, 864, 1120, 1555
HpySE526I ACGT 4 cut(s) 911, 1546, 1650, 1716
Hsp92I GRCGYC 2 cut(s) 237, 911
HspAI GCGC 3 cut(s) 429, 943, 956
Ksp22I TGATCA 2 cut(s) 802, 970
Kzo9I GATC 9 cut(s) 165, 802, 970, 1054, 1264, 1343, 1401, 1531, 1918
LmnI GCTCC 6 cut(s) 134, 230, 394, 1562, 1693, 1826
LweI GCATC 5 cut(s) 28, 778, 966, 1362, 1647
MaeI CTAG 2 cut(s) 933, 1448
MaeII ACGT 4 cut(s) 911, 1546, 1650, 1716
MaeIII GTNAC 6 cut(s) 70, 138, 251, 887, 1201, 1433
MalI GATC 9 cut(s) 167, 804, 972, 1056, 1266, 1345, 1403, 1533, 1920
MboI GATC 9 cut(s) 165, 802, 970, 1054, 1264, 1343, 1401, 1531, 1918
MboII GAAGA 3 cut(s) 1051, 1436, 1769
MfeI CAATTG 1 cut(s) 1910
MflI RGATCY 2 cut(s) 1054, 1264
MhlI GDGCHC 4 cut(s) 562, 1324, 1515, 1823
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 5 cut(s) 47, 258, 1231, 1665, 1671
MseI TTAA 4 cut(s) 420, 480, 525, 714
MslI CAYNNNNRTG 1 cut(s) 1338
MspA1I CMGCKG 1 cut(s) 1681
MspCI CTTAAG 1 cut(s) 419
MspI CCGG 4 cut(s) 240, 381, 432, 562
MspR9I CCNGG 3 cut(s) 116, 382, 562
MunI CAATTG 1 cut(s) 1910
Mva1269I GAATGC 1 cut(s) 1590
MvaI CCWGG 1 cut(s) 116
MwoI GCNNNNNNNGC 5 cut(s) 517, 1121, 1594, 1644, 1717
NciI CCSGG 2 cut(s) 382, 562
NcoI CCATGG 1 cut(s) 1642
NdeII GATC 9 cut(s) 165, 802, 970, 1054, 1264, 1343, 1401, 1531, 1918
NlaIV GGNNCC 4 cut(s) 1089, 1321, 1618, 1695
NmuCI GTSAC 3 cut(s) 70, 138, 1433
NsbI TGCGCA 1 cut(s) 957
NspI RCATGY 2 cut(s) 1231, 1877
PciI ACATGT 1 cut(s) 1227
PcsI WCGNNNNNNNCGW 1 cut(s) 608
PctI GAATGC 1 cut(s) 1590
PfeI GAWTC 2 cut(s) 1386, 1494
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
Ppu21I YACGTR 1 cut(s) 1717
PscI ACATGT 1 cut(s) 1227
PshBI ATTAAT 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 1823
Psp6I CCWGG 1 cut(s) 114
PspFI CCCAGC 1 cut(s) 1537
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 4 cut(s) 1089, 1321, 1618, 1695
PstNI CAGNNNCTG 1 cut(s) 58
PsuI RGATCY 2 cut(s) 1054, 1264
PvuII CAGCTG 1 cut(s) 1681
RsaI GTAC 3 cut(s) 5, 279, 302
RsaNI GTAC 3 cut(s) 4, 278, 301
RseI CAYNNNNRTG 1 cut(s) 1338
SacI GAGCTC 1 cut(s) 1823
SaqAI TTAA 4 cut(s) 420, 480, 525, 714
Sau3AI GATC 9 cut(s) 165, 802, 970, 1054, 1264, 1343, 1401, 1531, 1918
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 3 cut(s) 116, 382, 562
SduI GDGCHC 4 cut(s) 562, 1324, 1515, 1823
SfaNI GCATC 5 cut(s) 28, 778, 966, 1362, 1647
SfcI CTRYAG 2 cut(s) 1126, 1973
SmiMI CAYNNNNRTG 1 cut(s) 1338
SmlI CTYRAG 2 cut(s) 14, 419
SmoI CTYRAG 2 cut(s) 14, 419
SsiI CCGC 3 cut(s) 37, 112, 178
SspMI CTAG 2 cut(s) 933, 1448
SstI GAGCTC 1 cut(s) 1823
StyD4I CCNGG 3 cut(s) 114, 380, 560
StyI CCWWGG 2 cut(s) 1104, 1642
TaaI ACNGT 6 cut(s) 28, 1201, 1337, 1439, 1793, 1974
TaiI ACGT 4 cut(s) 914, 1549, 1653, 1719
TaqI TCGA 3 cut(s) 611, 876, 914
TatI WGTACW 3 cut(s) 3, 277, 300
TfiI GAWTC 2 cut(s) 1386, 1494
Tru1I TTAA 4 cut(s) 420, 480, 525, 714
Tru9I TTAA 4 cut(s) 420, 480, 525, 714
TscAI CASTG 4 cut(s) 987, 1204, 1444, 1578
TseFI GTSAC 3 cut(s) 70, 138, 1433
Tsp45I GTSAC 3 cut(s) 70, 138, 1433
TspDTI ATGAA 6 cut(s) 455, 591, 1399, 1493, 1607, 1863
TspGWI ACGGA 2 cut(s) 217, 239
TspRI CASTG 4 cut(s) 987, 1204, 1444, 1578
Vha464I CTTAAG 1 cut(s) 419
VspI ATTAAT 1 cut(s) 525
XapI RAATTY 7 cut(s) 93, 293, 376, 398, 607, 1003, 1850
XbaI TCTAGA 1 cut(s) 1447
XceI RCATGY 2 cut(s) 1231, 1877
XcmI CCANNNNNNNNNTGG 1 cut(s) 1172
XmiI GTMKAC 1 cut(s) 1224
XspI CTAG 2 cut(s) 933, 1448
ZraI GACGTC 1 cut(s) 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.