Rh2AG160800

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0017221
Physical Location & Seq
Reverse (-)
15180872 .. 15193210
12339 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG160800.1

Sequence Viewer

Length: 1842 bp
ATGTCACACCTCAAGCAACTCCACGCCCAGATCATCCTCCACCGCCTCACCAACCAAAACCTCACCCTCGGAAAGCTCATTTCCTTCTGCTCCGTCTCCGACGCCGGAGACCTCCGTTACGCTCACCTTGTGTTTGACCAAGTCCATCAACCCAATAAATTTATGTACAATAGTCTAATTAGAGGTTACTCAAATAGCAATGACTCATTCATGGCTATGTCTTTGTATTACCAAATGATAAATTCCGGGCTTGCACCGAACGAGTTCACATTGCCGTTTGTTCTTAAGGTTTGCGCCGGAAAAACGGCGTATTGGGAAGGCGTGGTGGTTCATGGCCAGGCTGTTAAGCTTGGAATTGGGTGTCAAGTTTGCGTGCAAAATGGGCTTATTAATGTGTATGGTGTTTGTGGGTTCATACATTTTGCCCGGAATGTGTTTGATGAAATGTCTGAGAGAAGTTTGGTGTCGTGGAATTCGATGATAGGTGGGTATGCTGGAGTTGGTTCTTGGAAGGAAGCTTTCGGATTGTTCAGGGAGATGAGGGATTTCGGCATTGAGCCTGATGAGTTCACATTGGTTAATTTACTTTCTGTTTGTTCACAGGCTTGTGATTTGGAGTTGGGGAGATATCTGCATCACTATATTGTGGTTGGCGGGATGGTGGTTGATCAGATTTTGAGAAATGCTCTGCTGGATATGTATGGTAAGTGTGGGCATTTGCCTTCGGCTCAGATGGTTTTCGACCAAATGAGTTACAAAAATGTGGTTTCTTGGACGTCGATGGTTAGGGCGTATGCTAGACATGGGCGCATTGAAGTTGCGCAGAAGTTTTTTGATCAGATGCCACTGAAAAATGTGGTTTCTTGGAATTCACTAATTTCGTGTTATGTGCGAGAAGGCCAATGCAGGGAAGCTCTGGATCTCTTCCAGAAAATGCTCAATTCTGGTGAGGTGCCTGATGAGGCTACCTTGGTTTTCACTCTCTCGGCCTGTAGTCAAATTGGCGATTTGGTCATTGGAAAGGAAACCCACAATTACATTAGCAACAGCAATATAGCACTCACTGTAACTCTTTCTAATTCCCTTGTAGACATGTATGCAAAATGTGGTGCTGTTGGAACTGCTATAGATCTTTTTAGTCAGATGCCAGAAAAGAATGTAGTATCATGGAACATTGTTATTGGTGCCCTTGCATTGCACGGTTATGGATCAGAAGCAATTAGGATATTTGAGCAGATGCAAGAAGGTGGAATCTGTCCTGATGAGATCACCTTCATCGGATTGCTTTCTGCTTGTAGTCACAGTGGTCTTCTAGACTTGGGGAGATATTACTTTGACAGAATGAAGTCTATTTACAGACTCTCTCCTGAAATTGAGCACTATGCTTGCATGGTTGATCTGCTGGGACGACGTGGGTGCTTAGAAGAAGCAATTACACTGATGAGAGGAATGCCAATGAAGCCTGATATTGTTGTTTGGGGTGCTATGCTTGGTGCTTGTCGCATCCATGGCAACATAGATTTAGCCAAGCTAATTCTAAAACAGCTGTTGGAGTTGGAGCCACATGGTTCTGGGCTTTACGTGCTTCTTGCAAACATATTTGGTGAAGCTCACAGATGGGAAGATGTCAAGAATATCAGGAAACTAATAAACGACGGTGGAGTCATCAAGTGTAGAGCTGTGAGCTCCATTGAAATTGATGGTTGTGTTTATGAATTTATGGTTGATGACAATAGACATGCAATTTCAAGCAGTATATACTCCATGCTCGATCAATTGACAGATCATCTGAAGTCTCTTGTGTATAATCCTACTGCAATGTGTGATTTAGAGGAACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

613

Amino Acids

68.46

Weight (kDa)

5.79

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 51 - 99 9.2e-08 PPR repeat family
PPR_2 PF13041 153 - 199 2.1e-11 PPR repeat family
PPR PF01535 155 - 185 2.2e-08 PPR repeat
PPR_2 PF13041 253 - 286 9.7e-06 PPR repeat family
PPR PF01535 256 - 285 2e-06 PPR repeat
PPR_2 PF13041 284 - 331 3.5e-11 PPR repeat family
PPR PF01535 287 - 316 1.6e-09 PPR repeat
PPR_2 PF13041 385 - 433 6.2e-11 PPR repeat family
PPR PF01535 388 - 418 7.2e-06 PPR repeat
E_motif PF20431 503 - 565 1.5e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017221)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g14410
malus_domestica MD02G1157600.v1.1
prunus_persica Prupe.7G146100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0103131
rosa_laevigata RLG00000017212
rosa_multiflora Rmu_sc0000388.1_g000006
rosa_roxburghii Rroxscaffold_2G00139900
rosa_rugosa Rorug02G0112000
rosa_samantha Rh2AG160800 Rh2BG167500 Rh2CG167800 Rh2DG166400
rosa_wichuraiana Rw2G012590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1661
AatII GACGTC 1 cut(s) 779
Acc16I TGCGCA 1 cut(s) 822
AccB1I GGYRCC 2 cut(s) 952, 1184
AccI GTMKAC 1 cut(s) 1089
AciI CCGC 2 cut(s) 43, 654
AclWI GGATC 2 cut(s) 927, 1216
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 5 cut(s) 158, 241, 472, 868, 1715
AcuI CTGAAG 1 cut(s) 1811
AcyI GRCGYC 2 cut(s) 102, 776
AdeI CACNNNGTG 1 cut(s) 130
AfaI GTAC 1 cut(s) 167
AfiI CCNNNNNNNGG 1 cut(s) 907
AflII CTTAAG 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 1092
AgsI TTSAA 3 cut(s) 815, 1694, 1749
AjiI CACGTC 1 cut(s) 1412
AjnI CCWGG 1 cut(s) 336
AluBI AGCT 9 cut(s) 76, 349, 518, 914, 1531, 1546, 1610, 1679, 1686
AluI AGCT 9 cut(s) 76, 349, 518, 914, 1531, 1546, 1610, 1679, 1686
Alw21I GWGCWC 2 cut(s) 1380, 1688
Alw26I GTCTC 3 cut(s) 100, 102, 1800
AlwI GGATC 2 cut(s) 927, 1216
AoxI GGCC 3 cut(s) 334, 898, 987
ApoI RAATTY 5 cut(s) 158, 241, 472, 868, 1715
AseI ATTAAT 1 cut(s) 390
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 3 cut(s) 296, 810, 823
AsuC2I CCSGG 2 cut(s) 247, 427
AsuHPI GGTGA 6 cut(s) 40, 55, 116, 959, 1261, 1616
BaeGI GKGCMC 1 cut(s) 1189
BalI TGGCCA 1 cut(s) 336
BanI GGYRCC 2 cut(s) 952, 1184
BanII GRGCYC 1 cut(s) 1688
BarI GAAGNNNNNNTAC 2 cut(s) 1337, 1369
BbsI GAAGAC 1 cut(s) 1301
Bbv12I GWGCWC 2 cut(s) 1380, 1688
BccI CCATC 6 cut(s) 153, 652, 727, 775, 1611, 1694
BceAI ACGGC 2 cut(s) 259, 321
BcgI CGANNNNNNTGC 2 cut(s) 1398, 1432
BciT130I CCWGG 1 cut(s) 338
BclI TGATCA 2 cut(s) 667, 835
BcnI CCSGG 2 cut(s) 247, 427
BcoDI GTCTC 3 cut(s) 100, 102, 1800
BfaI CTAG 2 cut(s) 798, 1313
BfmI CTRYAG 2 cut(s) 991, 1125
BfrI CTTAAG 1 cut(s) 284
BglII AGATCT 1 cut(s) 1129
Bme1390I CCNGG 3 cut(s) 247, 338, 427
BmgBI CACGTC 1 cut(s) 1412
BmiI GGNNCC 4 cut(s) 954, 1186, 1560, 1836
BmrFI CCNGG 3 cut(s) 247, 338, 427
BmsI GCATC 5 cut(s) 643, 831, 1134, 1227, 1512
BpiI GAAGAC 1 cut(s) 1301
BplI GAGNNNNNCTC 2 cut(s) 670, 702
BpmI CTGGAG 1 cut(s) 516
BpuMI CCSGG 2 cut(s) 247, 427
BsaAI YACGTR 1 cut(s) 1582
BsaHI GRCGYC 2 cut(s) 102, 776
BsaI GGTCTC 1 cut(s) 102
BsaJI CCNNGG 3 cut(s) 67, 969, 1507
Bsc4I CCNNNNNNNGG 1 cut(s) 907
Bse3DI GCAATG 4 cut(s) 205, 269, 1193, 1824
BseBI CCWGG 1 cut(s) 338
BseDI CCNNGG 3 cut(s) 67, 969, 1507
BseGI GGATG 3 cut(s) 33, 663, 1503
BseLI CCNNNNNNNGG 1 cut(s) 907
BseMI GCAATG 4 cut(s) 205, 269, 1193, 1824
BseMII CTCAG 2 cut(s) 441, 743
BseSI GKGCMC 1 cut(s) 1189
BseYI CCCAGC 1 cut(s) 1402
BshFI GGCC 3 cut(s) 336, 900, 989
BshNI GGYRCC 2 cut(s) 952, 1184
BsiHKAI GWGCWC 2 cut(s) 1380, 1688
BsiSI CCGG 4 cut(s) 105, 246, 297, 427
BslFI GGGAC 1 cut(s) 1419
BslI CCNNNNNNNGG 1 cut(s) 907
BsmAI GTCTC 3 cut(s) 100, 102, 1800
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 1419
BsmI GAATGC 1 cut(s) 1455
BsnI GGCC 3 cut(s) 336, 900, 989
Bso31I GGTCTC 1 cut(s) 102
Bsp1286I GDGCHC 3 cut(s) 1189, 1380, 1688
Bsp1407I TGTACA 1 cut(s) 165
Bsp19I CCATGG 1 cut(s) 1507
BspACI CCGC 2 cut(s) 43, 654
BspANI GGCC 3 cut(s) 336, 900, 989
BspCNI CTCAG 2 cut(s) 442, 742
BspLI GGNNCC 4 cut(s) 954, 1186, 1560, 1836
BspPI GGATC 2 cut(s) 927, 1216
BspT107I GGYRCC 2 cut(s) 952, 1184
BspTI CTTAAG 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 102
BsrDI GCAATG 4 cut(s) 205, 269, 1193, 1824
BsrGI TGTACA 1 cut(s) 165
BssECI CCNNGG 3 cut(s) 67, 969, 1507
BssNI GRCGYC 2 cut(s) 102, 776
BssT1I CCWWGG 2 cut(s) 969, 1507
Bst2UI CCWGG 1 cut(s) 338
Bst4CI ACNGT 5 cut(s) 1066, 1202, 1304, 1658, 1839
Bst6I CTCTTC 1 cut(s) 929
BstACI GRCGYC 2 cut(s) 102, 776
BstAFI CTTAAG 1 cut(s) 284
BstAUI TGTACA 1 cut(s) 165
BstBAI YACGTR 1 cut(s) 1582
BstC8I GCNNGC 3 cut(s) 252, 374, 1387
BstDEI CTNAG 3 cut(s) 450, 729, 1420
BstDSI CCRYGG 1 cut(s) 1507
BstF5I GGATG 3 cut(s) 33, 663, 1503
BstHHI GCGC 3 cut(s) 296, 810, 823
BstMAI GTCTC 3 cut(s) 100, 102, 1800
BstMWI GCNNNNNNNGC 4 cut(s) 382, 1459, 1509, 1582
BstNI CCWGG 1 cut(s) 338
BstNSI RCATGY 2 cut(s) 1096, 1742
BstSCI CCNGG 3 cut(s) 245, 336, 425
BstSFI CTRYAG 2 cut(s) 991, 1125
BstSLI GKGCMC 1 cut(s) 1189
BstV2I GAAGAC 1 cut(s) 1301
BstX2I RGATCY 2 cut(s) 919, 1129
BstYI RGATCY 2 cut(s) 919, 1129
BsuRI GGCC 3 cut(s) 336, 900, 989
BtgI CCRYGG 1 cut(s) 1507
BtrI CACGTC 1 cut(s) 1412
BtsCI GGATG 3 cut(s) 33, 663, 1503
BtsIMutI CAGTG 4 cut(s) 845, 1062, 1309, 1436
Cac8I GCNNGC 3 cut(s) 252, 374, 1387
CfoI GCGC 3 cut(s) 296, 810, 823
CseI GACGC 1 cut(s) 110
Csp6I GTAC 1 cut(s) 166
CviQI GTAC 1 cut(s) 166
DdeI CTNAG 3 cut(s) 450, 729, 1420
DraIII CACNNNGTG 1 cut(s) 130
DrdI GACNNNNNNGTC 1 cut(s) 1661
DseDI GACNNNNNNGTC 1 cut(s) 1661
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 929
EarI CTCTTC 1 cut(s) 929
Ecl136II GAGCTC 1 cut(s) 1686
Eco130I CCWWGG 2 cut(s) 969, 1507
Eco24I GRGCYC 1 cut(s) 1688
Eco31I GGTCTC 1 cut(s) 102
Eco32I GATATC 1 cut(s) 629
Eco53kI GAGCTC 1 cut(s) 1686
Eco57I CTGAAG 1 cut(s) 1811
EcoICRI GAGCTC 1 cut(s) 1686
EcoRI GAATTC 2 cut(s) 472, 868
EcoRII CCWGG 1 cut(s) 336
EcoRV GATATC 1 cut(s) 629
EcoT14I CCWWGG 2 cut(s) 969, 1507
EcoT38I GRGCYC 1 cut(s) 1688
ErhI CCWWGG 2 cut(s) 969, 1507
Esp3I CGTCTC 1 cut(s) 100
FaqI GGGAC 1 cut(s) 1419
FauI CCCGC 1 cut(s) 647
FbaI TGATCA 2 cut(s) 667, 835
FblI GTMKAC 1 cut(s) 1089
FokI GGATG 3 cut(s) 20, 670, 1490
FriOI GRGCYC 1 cut(s) 1688
FspBI CTAG 2 cut(s) 798, 1313
FspI TGCGCA 1 cut(s) 822
GlaI GCGC 3 cut(s) 295, 809, 822
GsaI CCCAGC 1 cut(s) 1406
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 3 cut(s) 336, 900, 989
HapII CCGG 4 cut(s) 105, 246, 297, 427
HgaI GACGC 1 cut(s) 110
HhaI GCGC 3 cut(s) 296, 810, 823
Hin1I GRCGYC 2 cut(s) 102, 776
Hin6I GCGC 3 cut(s) 294, 808, 821
HinP1I GCGC 3 cut(s) 294, 808, 821
HindIII AAGCTT 2 cut(s) 347, 516
HinfI GANTC 4 cut(s) 203, 1251, 1359, 1662
HpaII CCGG 4 cut(s) 105, 246, 297, 427
HphI GGTGA 6 cut(s) 40, 55, 116, 959, 1261, 1616
Hpy166II GTNNAC 4 cut(s) 267, 570, 599, 1090
Hpy188III TCNNGA 7 cut(s) 917, 928, 1259, 1313, 1367, 1630, 1639
Hpy8I GTNNAC 4 cut(s) 267, 570, 599, 1090
Hpy99I CGWCG 4 cut(s) 104, 781, 1413, 1658
HpyAV CCTTC 7 cut(s) 94, 311, 505, 732, 890, 1238, 1282
HpyCH4III ACNGT 5 cut(s) 1066, 1202, 1304, 1658, 1839
HpyCH4IV ACGT 3 cut(s) 776, 1411, 1581
HpyF10VI GCNNNNNNNGC 4 cut(s) 382, 1459, 1509, 1582
HpyF3I CTNAG 3 cut(s) 450, 729, 1420
HpySE526I ACGT 3 cut(s) 776, 1411, 1581
Hsp92I GRCGYC 2 cut(s) 102, 776
HspAI GCGC 3 cut(s) 294, 808, 821
Ksp22I TGATCA 2 cut(s) 667, 835
LmnI GCTCC 3 cut(s) 95, 1558, 1691
LweI GCATC 5 cut(s) 643, 831, 1134, 1227, 1512
MaeI CTAG 2 cut(s) 798, 1313
MaeII ACGT 3 cut(s) 776, 1411, 1581
MaeIII GTNAC 6 cut(s) 3, 116, 185, 752, 1066, 1298
MboII GAAGA 4 cut(s) 916, 1301, 1436, 1634
MfeI CAATTG 1 cut(s) 1775
MflI RGATCY 2 cut(s) 919, 1129
MhlI GDGCHC 3 cut(s) 1189, 1380, 1688
MlsI TGGCCA 1 cut(s) 336
MluNI TGGCCA 1 cut(s) 336
MlyI GAGTC 3 cut(s) 197, 1353, 1671
MmeI TCCRAC 4 cut(s) 123, 1096, 1530, 1536
Mox20I TGGCCA 1 cut(s) 336
MroXI GAANNNNTTC 1 cut(s) 263
MscI TGGCCA 1 cut(s) 336
MseI TTAA 4 cut(s) 285, 345, 390, 579
MslI CAYNNNNRTG 2 cut(s) 215, 1203
Msp20I TGGCCA 1 cut(s) 336
MspA1I CMGCKG 1 cut(s) 1546
MspCI CTTAAG 1 cut(s) 284
MspI CCGG 4 cut(s) 105, 246, 297, 427
MspR9I CCNGG 3 cut(s) 247, 338, 427
MunI CAATTG 1 cut(s) 1775
Mva1269I GAATGC 1 cut(s) 1455
MvaI CCWGG 1 cut(s) 338
MwoI GCNNNNNNNGC 4 cut(s) 382, 1459, 1509, 1582
NciI CCSGG 2 cut(s) 247, 427
NcoI CCATGG 1 cut(s) 1507
NlaIV GGNNCC 4 cut(s) 954, 1186, 1560, 1836
NmeAIII GCCGAG 1 cut(s) 965
NmuCI GTSAC 2 cut(s) 3, 1298
NsbI TGCGCA 1 cut(s) 822
NspI RCATGY 2 cut(s) 1096, 1742
PciI ACATGT 1 cut(s) 1092
PcsI WCGNNNNNNNCGW 1 cut(s) 473
PctI GAATGC 1 cut(s) 1455
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 1 cut(s) 1251
PflFI GACNNNGTC 1 cut(s) 140
PleI GAGTC 3 cut(s) 197, 1353, 1670
PpsI GAGTC 3 cut(s) 197, 1353, 1670
Ppu21I YACGTR 1 cut(s) 1582
PscI ACATGT 1 cut(s) 1092
PshBI ATTAAT 1 cut(s) 390
Psp124BI GAGCTC 1 cut(s) 1688
Psp6I CCWGG 1 cut(s) 336
PspFI CCCAGC 1 cut(s) 1402
PspGI CCWGG 1 cut(s) 336
PspN4I GGNNCC 4 cut(s) 954, 1186, 1560, 1836
PsuI RGATCY 2 cut(s) 919, 1129
PsyI GACNNNGTC 1 cut(s) 140
PvuII CAGCTG 1 cut(s) 1546
RsaI GTAC 1 cut(s) 167
RsaNI GTAC 1 cut(s) 166
RseI CAYNNNNRTG 2 cut(s) 215, 1203
SacI GAGCTC 1 cut(s) 1688
SaqAI TTAA 4 cut(s) 285, 345, 390, 579
SchI GAGTC 3 cut(s) 197, 1353, 1671
ScrFI CCNGG 3 cut(s) 247, 338, 427
SduI GDGCHC 3 cut(s) 1189, 1380, 1688
SfaNI GCATC 5 cut(s) 643, 831, 1134, 1227, 1512
SfcI CTRYAG 2 cut(s) 991, 1125
SmiMI CAYNNNNRTG 2 cut(s) 215, 1203
SmlI CTYRAG 2 cut(s) 11, 284
SmoI CTYRAG 2 cut(s) 11, 284
SsiI CCGC 2 cut(s) 43, 654
SspMI CTAG 2 cut(s) 798, 1313
SstI GAGCTC 1 cut(s) 1688
StyD4I CCNGG 3 cut(s) 245, 336, 425
StyI CCWWGG 2 cut(s) 969, 1507
TaaI ACNGT 5 cut(s) 1066, 1202, 1304, 1658, 1839
TaiI ACGT 3 cut(s) 779, 1414, 1584
TaqI TCGA 4 cut(s) 476, 741, 779, 1770
TatI WGTACW 1 cut(s) 165
TfiI GAWTC 1 cut(s) 1251
Tru1I TTAA 4 cut(s) 285, 345, 390, 579
Tru9I TTAA 4 cut(s) 285, 345, 390, 579
TscAI CASTG 4 cut(s) 852, 1069, 1309, 1443
TseFI GTSAC 2 cut(s) 3, 1298
Tsp45I GTSAC 2 cut(s) 3, 1298
TspDTI ATGAA 8 cut(s) 199, 320, 403, 456, 1264, 1358, 1472, 1728
TspGWI ACGGA 2 cut(s) 82, 104
TspRI CASTG 4 cut(s) 852, 1069, 1309, 1443
Tth111I GACNNNGTC 1 cut(s) 140
Vha464I CTTAAG 1 cut(s) 284
VspI ATTAAT 1 cut(s) 390
XapI RAATTY 5 cut(s) 158, 241, 472, 868, 1715
XbaI TCTAGA 1 cut(s) 1312
XceI RCATGY 2 cut(s) 1096, 1742
XmiI GTMKAC 1 cut(s) 1089
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 2 cut(s) 798, 1313
ZraI GACGTC 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.