Rroxscaffold_3G00233360

MINDY deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0019519
Physical Location & Seq
Reverse (-)
19321939 .. 19346621
24683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00233360.1

Sequence Viewer

Length: 654 bp
ATGGGATGTGGTAGGACAGAAAAGGGAGTTTCTGCTGTTGGACAAATGATTGCTCTCTATTTAAGATCAAACCTCAAGGAAGCTTGTGAAATCAGTGAAGATCCTAGCAACCAAGAACATTATGAAGCAAGAACATTGGTACAACATACAGGTGAGATGATATCCCCTTGCTTCAGTGGTTTGGAGCAAAACTTTCGTGGTAAAGAGAAAACCCGCATCCTTAGAGTTACCCTTACAACTCTTATAAAGTCTCAACCATTCCATCTATCAAATGCTTCATCAAAACTTACCACAAATCAATCCTTAGGATTTCATGATTCAGAGATTAAATCAAACTCCACATCACATACTCGGATTGCATTTTCTCGGTCTCACCGGATTTTCGCCATTTCCGGTGAAAACTACCACTTCCAGTCGATAGGTGGTGTAGAGGCGAAGGCATGGGAGGTGGTCTTGCGTCGAACGGAGGCCGGATGGTGGAGAACTTGGCCGAGGAGCTACCGCTCTACAAAAGATGCTGGCTACGTAGAAAACCAACAGCAAAACATAGCTGATGCTATCAATCTACTCCCTCGACTTGCAACCTATTTCGTTGTTAACATAAGGATAAGTGATTTTGAGTTTACTCCGAATGTGCAATATTTGATCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MINDY_DUB

Protein Analysis

217

Amino Acids

24.72

Weight (kDa)

9.19

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 245
AccBSI CCGCTC 1 cut(s) 504
AciI CCGC 2 cut(s) 214, 502
AclWI GGATC 2 cut(s) 95, 640
AcoI YGGCCR 1 cut(s) 488
AcuI CTGAAG 1 cut(s) 157
AfaI GTAC 1 cut(s) 141
AfiI CCNNNNNNNGG 1 cut(s) 477
AluBI AGCT 3 cut(s) 83, 498, 551
AluI AGCT 3 cut(s) 83, 498, 551
Alw26I GTCTC 2 cut(s) 255, 375
AlwI GGATC 2 cut(s) 95, 640
AoxI GGCC 2 cut(s) 468, 488
AsuHPI GGTGA 3 cut(s) 164, 365, 407
AxyI CCTNAGG 1 cut(s) 304
BccI CCATC 2 cut(s) 270, 468
BcgI CGANNNNNNTGC 2 cut(s) 176, 210
BcoDI GTCTC 2 cut(s) 255, 375
BfaI CTAG 1 cut(s) 105
BmsI GCATC 3 cut(s) 225, 505, 544
BpuEI CTTGAG 1 cut(s) 59
BsaAI YACGTR 1 cut(s) 526
BsaI GGTCTC 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 491
BsaWI WCCGGW 2 cut(s) 375, 392
Bsc4I CCNNNNNNNGG 1 cut(s) 477
Bse1I ACTGG 1 cut(s) 412
Bse21I CCTNAGG 1 cut(s) 304
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 3 cut(s) 11, 216, 479
BseLI CCNNNNNNNGG 1 cut(s) 477
BseNI ACTGG 1 cut(s) 412
BseRI GAGGAG 1 cut(s) 508
BshFI GGCC 2 cut(s) 470, 490
BsiSI CCGG 3 cut(s) 376, 393, 471
BslI CCNNNNNNNGG 1 cut(s) 477
BsmAI GTCTC 2 cut(s) 255, 375
BsnI GGCC 2 cut(s) 470, 490
Bso31I GGTCTC 1 cut(s) 375
Bsp143I GATC 3 cut(s) 65, 100, 645
BspACI CCGC 2 cut(s) 214, 502
BspANI GGCC 2 cut(s) 470, 490
BspHI TCATGA 1 cut(s) 313
BspPI GGATC 2 cut(s) 95, 640
BspTNI GGTCTC 1 cut(s) 375
BsrBI CCGCTC 1 cut(s) 504
BsrI ACTGG 1 cut(s) 412
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 3 cut(s) 65, 100, 645
BstBAI YACGTR 1 cut(s) 526
BstC8I GCNNGC 1 cut(s) 520
BstDEI CTNAG 2 cut(s) 221, 304
BstF5I GGATG 3 cut(s) 11, 216, 479
BstKTI GATC 3 cut(s) 68, 103, 648
BstMAI GTCTC 2 cut(s) 255, 375
BstMBI GATC 3 cut(s) 65, 100, 645
BstSNI TACGTA 1 cut(s) 526
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
Bsu36I CCTNAGG 1 cut(s) 304
BsuRI GGCC 2 cut(s) 470, 490
BtsCI GGATG 3 cut(s) 11, 216, 479
BtsIMutI CAGTG 2 cut(s) 100, 181
Cac8I GCNNGC 1 cut(s) 520
CciI TCATGA 1 cut(s) 313
CseI GACGC 1 cut(s) 446
Csp6I GTAC 1 cut(s) 140
CviAII CATG 2 cut(s) 314, 441
CviJI RGCY 6 cut(s) 83, 470, 490, 498, 522, 551
CviKI_1 RGCY 6 cut(s) 83, 470, 490, 498, 522, 551
CviQI GTAC 1 cut(s) 140
DdeI CTNAG 2 cut(s) 221, 304
DpnI GATC 3 cut(s) 67, 102, 647
DpnII GATC 3 cut(s) 65, 100, 645
EaeI YGGCCR 1 cut(s) 488
Eco105I TACGTA 1 cut(s) 526
Eco31I GGTCTC 1 cut(s) 375
Eco32I GATATC 1 cut(s) 162
Eco57I CTGAAG 1 cut(s) 157
Eco81I CCTNAGG 1 cut(s) 304
EcoRV GATATC 1 cut(s) 162
FaeI CATG 2 cut(s) 317, 444
FaiI YATR 8 cut(s) 123, 147, 245, 315, 348, 442, 548, 602
FatI CATG 2 cut(s) 313, 440
FauI CCCGC 1 cut(s) 221
FokI GGATG 3 cut(s) 18, 203, 486
FspBI CTAG 1 cut(s) 105
HaeIII GGCC 2 cut(s) 470, 490
HapII CCGG 3 cut(s) 376, 393, 471
HgaI GACGC 1 cut(s) 446
Hin1II CATG 2 cut(s) 317, 444
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 1 cut(s) 81
HinfI GANTC 1 cut(s) 317
HpaI GTTAAC 1 cut(s) 598
HpaII CCGG 3 cut(s) 376, 393, 471
HphI GGTGA 3 cut(s) 164, 365, 407
Hpy166II GTNNAC 2 cut(s) 598, 624
Hpy188I TCNGA 3 cut(s) 322, 354, 630
Hpy188III TCNNGA 1 cut(s) 314
Hpy8I GTNNAC 2 cut(s) 598, 624
Hpy99I CGWCG 1 cut(s) 462
HpyAV CCTTC 1 cut(s) 430
HpyCH4IV ACGT 1 cut(s) 525
HpyCH4V TGCA 3 cut(s) 359, 581, 637
HpyF3I CTNAG 2 cut(s) 221, 304
HpySE526I ACGT 1 cut(s) 525
Hsp92II CATG 2 cut(s) 317, 444
KspAI GTTAAC 1 cut(s) 598
Kzo9I GATC 3 cut(s) 65, 100, 645
LmnI GCTCC 2 cut(s) 184, 495
LpnPI CCDG 6 cut(s) 135, 389, 406, 425, 484, 504
LweI GCATC 3 cut(s) 225, 505, 544
MaeI CTAG 1 cut(s) 105
MaeII ACGT 1 cut(s) 525
MaeIII GTNAC 1 cut(s) 226
MalI GATC 3 cut(s) 67, 102, 647
MbiI CCGCTC 1 cut(s) 504
MboI GATC 3 cut(s) 65, 100, 645
MboII GAAGA 1 cut(s) 110
MflI RGATCY 1 cut(s) 100
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 6 cut(s) 83, 424, 439, 460, 486, 582
MseI TTAA 3 cut(s) 62, 327, 597
MslI CAYNNNNRTG 1 cut(s) 150
MspI CCGG 3 cut(s) 376, 393, 471
NdeII GATC 3 cut(s) 65, 100, 645
NlaIII CATG 2 cut(s) 317, 444
NmeAIII GCCGAG 1 cut(s) 516
PagI TCATGA 1 cut(s) 313
PfeI GAWTC 1 cut(s) 317
Ppu21I YACGTR 1 cut(s) 526
PsiI TTATAA 1 cut(s) 245
PsuI RGATCY 1 cut(s) 100
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
RseI CAYNNNNRTG 1 cut(s) 150
SaqAI TTAA 3 cut(s) 62, 327, 597
Sau3AI GATC 3 cut(s) 65, 100, 645
SetI ASST 9 cut(s) 75, 85, 154, 424, 450, 500, 528, 553, 587
SfaNI GCATC 3 cut(s) 225, 505, 544
SmiMI CAYNNNNRTG 1 cut(s) 150
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
SnaBI TACGTA 1 cut(s) 526
SsiI CCGC 2 cut(s) 214, 502
SspI AATATT 1 cut(s) 641
SspMI CTAG 1 cut(s) 105
TaiI ACGT 1 cut(s) 528
TaqI TCGA 3 cut(s) 416, 460, 574
TaqII GACCGA 1 cut(s) 357
TfiI GAWTC 1 cut(s) 317
Tru1I TTAA 3 cut(s) 62, 327, 597
Tru9I TTAA 3 cut(s) 62, 327, 597
TscAI CASTG 2 cut(s) 100, 181
TspDTI ATGAA 3 cut(s) 138, 267, 302
TspGWI ACGGA 2 cut(s) 479, 638
TspRI CASTG 2 cut(s) 100, 181
XcmI CCANNNNNNNNNTGG 1 cut(s) 419
XspI CTAG 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.