Rroxscaffold_3G00244400

Long-chain-alcohol oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0015043
Physical Location & Seq
Reverse (-)
37086011 .. 37090183
4173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00244400.1

Sequence Viewer

Length: 2376 bp
ATGGAGCAAAGTGAAAATGAAAAACATGCAGAAGATATTGAGCTTGGTGGCGTTGATGCAAAGAAGCTTGTTGGAGGATTTGTGTACCCAAACTCTCTCTCGTCCCGTGAAATGGAGTCGCTCACTGCCCTTTGTGACACCATCTTGCCCTCTACAGACGTCTCCGGTGACGCAAGCACTTGCACCGATGAGTCCTTGATCACGTTTTACGGGACATCAGCGTCCATGGCAGGAACACCGGATCGAGTAAGTAAATCTCGATCGTCTTTACTTGGTGCATGGTTATTACTTGTTGGTATTGTTGTTATTGATCTACATGTTTCCCATGTTCTTCTAGGGGGTTTGATAAGTGAGAGGCTAGAGCATCCAATGAAGTGGTTGATGCGATTATCATTATGGCTCTTGTCAACATGGATAGGAACCTTCATATTATGTGGAAGAGGCAGCTTGTCAACCCAATTTCCCTACTTTCGGAGTTTTGCCCAAGTTTCTCAGCAGAAACGAGAACAGATAGTGGTGTCATGGTCTCTCAGTTACTTTCATCTCCTTCGGATGTTCTTCAAGACCATCAAGCTTCTCACTCTCCTCGTCTTCTTCACTCAAGTGGATGAGAATGAGGAGAACCTATCTTGGAAAGCAATTGGCTACACGGGACCTGATCCAGATATCCTTGACAAAACCAATCTATCAACAGCATCGACAAGAAACGGATATGAAAGAGCAGACACTGAAGAACAAGAATATCAAAGAACTTACAAAGAAGAATTCTTTGGACCTCTTTACCATGGCATCATCAATTTGAGCAAACCAAAGGATGTAGTTGCCAATAGTCTGATGAGATTTGGAATTCCAGTCTCCATTATTTACTCGCCAAAAACATCCTCCGACCCGTCTTTAATTTTCCGATGCGATGCTGTAGTAATTGGTTCAGGCTCTGGCGGTGGTGTTGTTGCTGGCGTTCTGGCCAAGGCTGGTTACAAAGTGATCGTTTTGGACAAGGAGACTATCATGCCAGAAAGAACCTTACACTTCTTGAAGGACCCACAATGGATCAAATGCTCTACAATCAACTGGTCAGCCTCAATTCGAACACCTCAGCATGTAAGTAGCGAATGGTGCAATCACCATGGGTTAGAACTGTTCGACAGTCAACTGTATCAAGAAGCCATGGATATTGTCTGCCAACGAATGGGAGTTCAATCTGACATACATGAAGAAGGATTCAACAATGCTGTTTTGAGAAGGGGGTGCCAAGAATTGGGGTATCCTGTACATAATATTCCTCGAAACGCAGGACCAGATCACTATTGTGGTTGGTGCTGCCTCGGCTGCAAGGATGGAAGCAAAAAGGGTACATCAGAGACATGGCTGGTGGACTTGGTTCGTTCAGGAAACGGGGCAATTTTTTCAGGATGCGAGGCTATAAAAGTATTGCATACGAGAACGAAAGGTAGAGCTCGAAACACAGCTACTGGAGTCGTGTTTGAATTCAAACATGAAGGAGCAAAACAAGTCTGTGTGGTGGAATCTAAGGTCACAATTGTTGCTTGCGGTGCTCTCAGTACTCCAATATTGTTGAATCGAAGCGGATTGAAGAATGCCAATATTGGAAAAAATTTACATCTACATCCAGTTGCAATGTCATGGGGGTATTTTAATAATCCAGATTCACCTAATCTCTCTGAGAAAAAGAGCTACGAAGGAGGGATAATGACAGCAATGTCTACTGTCACTGCAGATTTTGACAGGTCTGGCTATGGAGCGTTGATACAAACACCTGCATTGCACCCTGGTATGTTCTCAATTGTAATGCCATGGATTTCAGGCAAAGATATAAAGCACCGAATGAGCAGGTTCTCTAAAACTGCCCACATATTTGCATTGGCAAGAGATAAAGGATCGGGGACAGTACTAACAAATGCACCAAACTCAGTCAGCTATCAAATGGAACCCGTTGATGAAAACAATCTAAACAAAGGACTTGAGAAGTCATTGAGGATTTTGGCGGCAGCGGGAGCTGAAGAAATTGGAACCCATCATTTCAAAGGGAAGAGTTTAAATGTGAAGAAGGCAAGTTCTAGTGAGTTTGAGTTTGAGCAGTTTGTGAAAGAGGAGAGTTCGAGGCCATTAGGAGACATTTCATCTAGCATCTGCTCTGCGCATCAGATGGGAAGTTGCAGAATGGGAGTCAACGCAAAAGGGTCGGTAGTCAACCAGATGGGAGAGACGTGGGAAGTTGAGGGACTATTTGTAGCAGACTCGAGTGTTTTCCCAACGGCTTTGGGAGTCAATCCTATGGTTACTGTTCAGTGTATTGCTTACTGCACTGCGCAATCTGTTATTCAAGTGCTTCAGAGAAGAAAGCCGGATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0008150 GO:0008152 GO:0016491 GO:0044464 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

791

Amino Acids

86.98

Weight (kDa)

6.77

Isoelectric Point (pI)

33.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 354 - 545 6.1e-59 GMC oxidoreductase
GMC_oxred_C PF05199 645 - 773 2.4e-21 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015043)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19380 AT4G19380
malus_domestica MD16G1286200.v1.1
prunus_persica Prupe.5G013900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0215241
rosa_laevigata RLG00000002680
rosa_multiflora Rmu_co8143572.1_g000001 Rmu_co8276433.1_g000001
rosa_roxburghii Rroxscaffold_3G00244400
rosa_rugosa Rorug07G0151400
rosa_samantha Rh7AG288100 Rh7BG280000 Rh7BG280100 Rh7CG307900 Rh7DG291600
rosa_wichuraiana Rw7G024610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1786
AasI GACNNNNNNGTC 2 cut(s) 220, 1720
AatII GACGTC 1 cut(s) 162
Acc16I TGCGCA 2 cut(s) 2160, 2331
Acc36I ACCTGC 2 cut(s) 1786, 1842
AccB1I GGYRCC 1 cut(s) 1248
AccB7I CCANNNNNTGG 2 cut(s) 1189, 1258
AccI GTMKAC 1 cut(s) 1724
AciI CCGC 5 cut(s) 939, 1551, 1587, 2006, 2012
AclWI GGATC 4 cut(s) 249, 653, 1058, 1906
AcoI YGGCCR 1 cut(s) 963
AcsI RAATTY 4 cut(s) 764, 846, 1487, 1615
AcuI CTGAAG 3 cut(s) 750, 2040, 2336
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 5 cut(s) 86, 1272, 1354, 1564, 1911
AfiI CCNNNNNNNGG 4 cut(s) 112, 471, 1189, 1258
AflIII ACRYGT 1 cut(s) 316
AjiI CACGTC 1 cut(s) 2229
AjnI CCWGG 1 cut(s) 1789
AjuI GAANNNNNNNTTGG 2 cut(s) 753, 785
AleI CACNNNNGTG 2 cut(s) 602, 1308
AluBI AGCT 9 cut(s) 43, 67, 447, 574, 1457, 1469, 1695, 1938, 2018
AluI AGCT 9 cut(s) 43, 67, 447, 574, 1457, 1469, 1695, 1938, 2018
Alw21I GWGCWC 2 cut(s) 1459, 1558
Alw26I GTCTC 7 cut(s) 166, 531, 859, 995, 1355, 2127, 2219
AlwI GGATC 4 cut(s) 249, 653, 1058, 1906
AlwNI CAGNNNCTG 3 cut(s) 728, 935, 1472
Ama87I CYCGRG 1 cut(s) 2260
AoxI GGCC 2 cut(s) 963, 2123
ApeKI GCWGC 4 cut(s) 444, 1320, 1329, 2009
ApoI RAATTY 4 cut(s) 764, 846, 1487, 1615
AspLEI GCGC 2 cut(s) 2161, 2332
AspS9I GGNCC 4 cut(s) 653, 773, 1039, 1295
AsuHPI GGTGA 3 cut(s) 179, 1115, 1662
AsuII TTCGAA 1 cut(s) 1087
AvaI CYCGRG 1 cut(s) 2260
AvaII GGWCC 4 cut(s) 653, 773, 1039, 1295
BalI TGGCCA 1 cut(s) 965
BanI GGYRCC 1 cut(s) 1248
BanII GRGCYC 1 cut(s) 1459
BbsI GAAGAC 1 cut(s) 583
Bbv12I GWGCWC 2 cut(s) 1459, 1558
BbvCI CCTCAGC 1 cut(s) 1095
BbvI GCAGC 4 cut(s) 456, 1307, 1316, 2021
BccI CCATC 6 cut(s) 149, 575, 1331, 2043, 2161, 2212
BceAI ACGGC 1 cut(s) 2292
BcgI CGANNNNNNTGC 2 cut(s) 2184, 2218
BciT130I CCWGG 1 cut(s) 1791
BciVI GTATCC 1 cut(s) 1275
BclI TGATCA 1 cut(s) 198
BcoDI GTCTC 7 cut(s) 166, 531, 859, 995, 1355, 2127, 2219
BfaI CTAG 4 cut(s) 335, 359, 2079, 2145
BfmI CTRYAG 3 cut(s) 153, 915, 1734
BfuAI ACCTGC 2 cut(s) 1786, 1842
BfuI GTATCC 1 cut(s) 1275
BisI GCNGC 5 cut(s) 445, 1321, 1330, 2007, 2010
BlsI GCNGC 5 cut(s) 446, 1322, 1331, 2008, 2011
BmcAI AGTACT 2 cut(s) 1564, 1911
Bme1390I CCNGG 1 cut(s) 1791
Bme18I GGWCC 4 cut(s) 653, 773, 1039, 1295
BmeT110I CYCGRG 1 cut(s) 2260
BmgBI CACGTC 1 cut(s) 2229
BmgT120I GGNCC 4 cut(s) 653, 773, 1039, 1295
BmiI GGNNCC 6 cut(s) 421, 654, 1041, 1250, 1950, 2032
BmrFI CCNGG 1 cut(s) 1791
BpiI GAAGAC 1 cut(s) 583
BpmI CTGGAG 1 cut(s) 1494
Bpu10I CCTNAGC 1 cut(s) 1095
Bpu14I TTCGAA 1 cut(s) 1087
BpuEI CTTGAG 2 cut(s) 585, 2003
BsaHI GRCGYC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 531
BsaJI CCNNGG 8 cut(s) 225, 784, 966, 1126, 1167, 1324, 1789, 1814
BsaWI WCCGGW 2 cut(s) 164, 238
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 4 cut(s) 112, 471, 1189, 1258
Bse1I ACTGG 4 cut(s) 851, 1076, 1477, 1631
Bse3DI GCAATG 3 cut(s) 1644, 1725, 1781
BseBI CCWGG 1 cut(s) 1791
BseDI CCNNGG 8 cut(s) 225, 784, 966, 1126, 1167, 1324, 1789, 1814
BseGI GGATG 9 cut(s) 364, 558, 613, 820, 878, 1342, 1418, 1627, 2374
BseLI CCNNNNNNNGG 4 cut(s) 112, 471, 1189, 1258
BseMI GCAATG 3 cut(s) 1644, 1725, 1781
BseMII CTCAG 6 cut(s) 506, 544, 1109, 1573, 1674, 1944
BseNI ACTGG 4 cut(s) 851, 1076, 1477, 1631
BseRI GAGGAG 3 cut(s) 575, 632, 2126
BseXI GCAGC 4 cut(s) 456, 1307, 1316, 2021
BsgI GTGCAG 1 cut(s) 2308
Bsh1285I CGRYCG 1 cut(s) 263
BshFI GGCC 2 cut(s) 965, 2125
BshNI GGYRCC 1 cut(s) 1248
BsiEI CGRYCG 1 cut(s) 263
BsiHKAI GWGCWC 2 cut(s) 1459, 1558
BsiHKCI CYCGRG 1 cut(s) 2260
BsiSI CCGG 3 cut(s) 165, 239, 2366
BslFI GGGAC 5 cut(s) 88, 226, 666, 1918, 2256
BslI CCNNNNNNNGG 4 cut(s) 112, 471, 1189, 1258
BsmAI GTCTC 7 cut(s) 166, 531, 859, 995, 1355, 2127, 2219
BsmBI CGTCTC 2 cut(s) 166, 2219
BsmFI GGGAC 5 cut(s) 88, 226, 666, 1918, 2256
BsmI GAATGC 1 cut(s) 1603
BsnI GGCC 2 cut(s) 965, 2125
Bso31I GGTCTC 1 cut(s) 531
BsoBI CYCGRG 1 cut(s) 2260
Bsp119I TTCGAA 1 cut(s) 1087
Bsp1286I GDGCHC 2 cut(s) 1459, 1558
Bsp1407I TGTACA 1 cut(s) 1270
Bsp143I GATC 9 cut(s) 198, 241, 260, 310, 658, 984, 1050, 1300, 1898
Bsp19I CCATGG 5 cut(s) 225, 784, 1126, 1167, 1814
BspACI CCGC 5 cut(s) 939, 1551, 1587, 2006, 2012
BspANI GGCC 2 cut(s) 965, 2125
BspCNI CTCAG 6 cut(s) 505, 543, 1108, 1572, 1675, 1943
BspLI GGNNCC 6 cut(s) 421, 654, 1041, 1250, 1950, 2032
BspMAI CTGCAG 1 cut(s) 1738
BspMI ACCTGC 2 cut(s) 1786, 1842
BspPI GGATC 4 cut(s) 249, 653, 1058, 1906
BspT104I TTCGAA 1 cut(s) 1087
BspT107I GGYRCC 1 cut(s) 1248
BspTNI GGTCTC 1 cut(s) 531
BsrDI GCAATG 3 cut(s) 1644, 1725, 1781
BsrGI TGTACA 1 cut(s) 1270
BsrI ACTGG 4 cut(s) 851, 1076, 1477, 1631
BssECI CCNNGG 8 cut(s) 225, 784, 966, 1126, 1167, 1324, 1789, 1814
BssMI GATC 9 cut(s) 198, 241, 260, 310, 658, 984, 1050, 1300, 1898
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 6 cut(s) 225, 784, 966, 1126, 1167, 1814
Bst2UI CCWGG 1 cut(s) 1791
Bst4CI ACNGT 6 cut(s) 1140, 1148, 1155, 1729, 1909, 2305
Bst6I CTCTTC 2 cut(s) 433, 2045
BstACI GRCGYC 1 cut(s) 159
BstAUI TGTACA 1 cut(s) 1270
BstBI TTCGAA 1 cut(s) 1087
BstC8I GCNNGC 3 cut(s) 175, 955, 1549
BstDEI CTNAG 7 cut(s) 492, 530, 1095, 1530, 1559, 1683, 1930
BstDSI CCRYGG 5 cut(s) 225, 784, 1126, 1167, 1814
BstF5I GGATG 9 cut(s) 364, 558, 613, 820, 878, 1342, 1418, 1627, 2374
BstHHI GCGC 2 cut(s) 2161, 2332
BstKTI GATC 9 cut(s) 201, 244, 263, 313, 661, 987, 1053, 1303, 1901
BstMAI GTCTC 7 cut(s) 166, 531, 859, 995, 1355, 2127, 2219
BstMBI GATC 9 cut(s) 198, 241, 260, 310, 658, 984, 1050, 1300, 1898
BstMCI CGRYCG 1 cut(s) 263
BstMWI GCNNNNNNNGC 6 cut(s) 227, 1116, 1326, 1329, 1553, 2015
BstNI CCWGG 1 cut(s) 1791
BstNSI RCATGY 3 cut(s) 29, 320, 1103
BstSCI CCNGG 1 cut(s) 1789
BstSFI CTRYAG 3 cut(s) 153, 915, 1734
BstV1I GCAGC 4 cut(s) 456, 1307, 1316, 2021
BstV2I GAAGAC 1 cut(s) 583
BstXI CCANNNNNNTGG 1 cut(s) 375
BsuI GTATCC 1 cut(s) 1275
BsuRI GGCC 2 cut(s) 965, 2125
BtgI CCRYGG 5 cut(s) 225, 784, 1126, 1167, 1814
BtgZI GCGATG 1 cut(s) 924
BtrI CACGTC 1 cut(s) 2229
BtsCI GGATG 9 cut(s) 364, 558, 613, 820, 878, 1342, 1418, 1627, 2374
BtsI GCAGTG 3 cut(s) 123, 1731, 2325
BtsIMutI CAGTG 5 cut(s) 123, 726, 1731, 2315, 2325
BveI ACCTGC 2 cut(s) 1786, 1842
Cac8I GCNNGC 3 cut(s) 175, 955, 1549
CaiI CAGNNNCTG 3 cut(s) 728, 935, 1472
CfoI GCGC 2 cut(s) 2161, 2332
Cfr13I GGNCC 4 cut(s) 653, 773, 1039, 1295
CseI GACGC 2 cut(s) 179, 210
Csp6I GTAC 5 cut(s) 85, 1271, 1353, 1563, 1910
CviQI GTAC 5 cut(s) 85, 1271, 1353, 1563, 1910
DdeI CTNAG 7 cut(s) 492, 530, 1095, 1530, 1559, 1683, 1930
DpnI GATC 9 cut(s) 200, 243, 262, 312, 660, 986, 1052, 1302, 1900
DpnII GATC 9 cut(s) 198, 241, 260, 310, 658, 984, 1050, 1300, 1898
DraI TTTAAA 1 cut(s) 2058
DrdI GACNNNNNNGTC 2 cut(s) 220, 1720
DseDI GACNNNNNNGTC 2 cut(s) 220, 1720
EaeI YGGCCR 1 cut(s) 963
Eam1104I CTCTTC 2 cut(s) 433, 2045
EarI CTCTTC 2 cut(s) 433, 2045
Ecl136II GAGCTC 1 cut(s) 1457
Eco130I CCWWGG 6 cut(s) 225, 784, 966, 1126, 1167, 1814
Eco24I GRGCYC 1 cut(s) 1459
Eco31I GGTCTC 1 cut(s) 531
Eco32I GATATC 1 cut(s) 667
Eco47I GGWCC 4 cut(s) 653, 773, 1039, 1295
Eco53kI GAGCTC 1 cut(s) 1457
Eco57I CTGAAG 3 cut(s) 750, 2040, 2336
Eco88I CYCGRG 1 cut(s) 2260
EcoICRI GAGCTC 1 cut(s) 1457
EcoO109I RGGNCCY 2 cut(s) 653, 1039
EcoRI GAATTC 3 cut(s) 764, 846, 1487
EcoRII CCWGG 1 cut(s) 1789
EcoRV GATATC 1 cut(s) 667
EcoT14I CCWWGG 6 cut(s) 225, 784, 966, 1126, 1167, 1814
EcoT38I GRGCYC 1 cut(s) 1459
ErhI CCWWGG 6 cut(s) 225, 784, 966, 1126, 1167, 1814
Esp3I CGTCTC 2 cut(s) 166, 2219
FaqI GGGAC 5 cut(s) 88, 226, 666, 1918, 2256
FauI CCCGC 1 cut(s) 2005
FbaI TGATCA 1 cut(s) 198
FblI GTMKAC 1 cut(s) 1724
Fnu4HI GCNGC 5 cut(s) 445, 1321, 1330, 2007, 2010
FokI GGATG 8 cut(s) 351, 565, 620, 827, 865, 1349, 1425, 1614
FriOI GRGCYC 1 cut(s) 1459
Fsp4HI GCNGC 5 cut(s) 445, 1321, 1330, 2007, 2010
FspBI CTAG 4 cut(s) 335, 359, 2079, 2145
FspI TGCGCA 2 cut(s) 2160, 2331
GlaI GCGC 2 cut(s) 2160, 2331
GluI GCNGC 5 cut(s) 445, 1321, 1330, 2007, 2010
GsuI CTGGAG 1 cut(s) 1494
HaeIII GGCC 2 cut(s) 965, 2125
HapII CCGG 3 cut(s) 165, 239, 2366
HgaI GACGC 2 cut(s) 179, 210
HhaI GCGC 2 cut(s) 2161, 2332
Hin1I GRCGYC 1 cut(s) 159
Hin6I GCGC 2 cut(s) 2159, 2330
HinP1I GCGC 2 cut(s) 2159, 2330
HincII GTYRAC 5 cut(s) 408, 453, 1151, 2191, 2212
HindII GTYRAC 5 cut(s) 408, 453, 1151, 2191, 2212
HindIII AAGCTT 2 cut(s) 65, 572
HpaII CCGG 3 cut(s) 165, 239, 2366
HphI GGTGA 3 cut(s) 179, 1115, 1662
Hpy166II GTNNAC 8 cut(s) 85, 408, 453, 1151, 1375, 1725, 2191, 2212
Hpy188III TCNNGA 8 cut(s) 258, 562, 662, 1033, 1160, 1389, 1410, 1664
Hpy8I GTNNAC 8 cut(s) 85, 408, 453, 1151, 1375, 1725, 2191, 2212
HpyAV CCTTC 8 cut(s) 433, 557, 1030, 1211, 1236, 1493, 1694, 2062
HpyCH4III ACNGT 6 cut(s) 1140, 1148, 1155, 1729, 1909, 2305
HpyCH4IV ACGT 3 cut(s) 159, 203, 2228
HpyF10VI GCNNNNNNNGC 6 cut(s) 227, 1116, 1326, 1329, 1553, 2015
HpyF3I CTNAG 7 cut(s) 492, 530, 1095, 1530, 1559, 1683, 1930
HpySE526I ACGT 3 cut(s) 159, 203, 2228
Hsp92I GRCGYC 1 cut(s) 159
HspAI GCGC 2 cut(s) 2159, 2330
Ksp22I TGATCA 1 cut(s) 198
Kzo9I GATC 9 cut(s) 198, 241, 260, 310, 658, 984, 1050, 1300, 1898
LmnI GCTCC 4 cut(s) 4, 1502, 1760, 2015
Lsp1109I GCAGC 4 cut(s) 456, 1307, 1316, 2021
MaeI CTAG 4 cut(s) 335, 359, 2079, 2145
MaeII ACGT 3 cut(s) 159, 203, 2228
MaeIII GTNAC 7 cut(s) 134, 167, 533, 974, 1534, 1729, 2299
MalI GATC 9 cut(s) 200, 243, 262, 312, 660, 986, 1052, 1302, 1900
MboI GATC 9 cut(s) 198, 241, 260, 310, 658, 984, 1050, 1300, 1898
MfeI CAATTG 3 cut(s) 639, 1539, 1803
MhlI GDGCHC 2 cut(s) 1459, 1558
MlsI TGGCCA 1 cut(s) 965
MluNI TGGCCA 1 cut(s) 965
MlyI GAGTC 6 cut(s) 125, 200, 1485, 2196, 2252, 2295
MmeI TCCRAC 2 cut(s) 52, 909
Mox20I TGGCCA 1 cut(s) 965
MscI TGGCCA 1 cut(s) 965
MseI TTAA 3 cut(s) 896, 1656, 2057
MslI CAYNNNNRTG 2 cut(s) 602, 1308
Msp20I TGGCCA 1 cut(s) 965
MspA1I CMGCKG 1 cut(s) 2012
MspI CCGG 3 cut(s) 165, 239, 2366
MspR9I CCNGG 1 cut(s) 1791
MunI CAATTG 3 cut(s) 639, 1539, 1803
Mva1269I GAATGC 1 cut(s) 1603
MvaI CCWGG 1 cut(s) 1791
MwoI GCNNNNNNNGC 6 cut(s) 227, 1116, 1326, 1329, 1553, 2015
NcoI CCATGG 5 cut(s) 225, 784, 1126, 1167, 1814
NdeII GATC 9 cut(s) 198, 241, 260, 310, 658, 984, 1050, 1300, 1898
NlaIV GGNNCC 6 cut(s) 421, 654, 1041, 1250, 1950, 2032
NmeAIII GCCGAG 1 cut(s) 1305
NmuCI GTSAC 4 cut(s) 134, 167, 1534, 1729
NsbI TGCGCA 2 cut(s) 2160, 2331
NspI RCATGY 3 cut(s) 29, 320, 1103
NspV TTCGAA 1 cut(s) 1087
OliI CACNNNNGTG 2 cut(s) 602, 1308
PaeR7I CTCGAG 1 cut(s) 2260
PaqCI CACCTGC 1 cut(s) 1786
PciI ACATGT 1 cut(s) 316
PctI GAATGC 1 cut(s) 1603
PfeI GAWTC 4 cut(s) 1221, 1526, 1579, 1667
PflMI CCANNNNNTGG 2 cut(s) 1189, 1258
PkrI GCNGC 5 cut(s) 446, 1322, 1331, 2008, 2011
Ple19I CGATCG 1 cut(s) 263
PleI GAGTC 6 cut(s) 124, 199, 1484, 2195, 2252, 2294
PpsI GAGTC 6 cut(s) 124, 199, 1484, 2195, 2252, 2294
PpuMI RGGWCCY 2 cut(s) 653, 1039
PscI ACATGT 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 1459
Psp5II RGGWCCY 2 cut(s) 653, 1039
Psp6I CCWGG 1 cut(s) 1789
PspGI CCWGG 1 cut(s) 1789
PspN4I GGNNCC 6 cut(s) 421, 654, 1041, 1250, 1950, 2032
PspPI GGNCC 4 cut(s) 653, 773, 1039, 1295
PspPPI RGGWCCY 2 cut(s) 653, 1039
PspXI VCTCGAGB 1 cut(s) 2260
PstI CTGCAG 1 cut(s) 1738
PstNI CAGNNNCTG 3 cut(s) 728, 935, 1472
PvuI CGATCG 1 cut(s) 263
RsaI GTAC 5 cut(s) 86, 1272, 1354, 1564, 1911
RsaNI GTAC 5 cut(s) 85, 1271, 1353, 1563, 1910
RseI CAYNNNNRTG 2 cut(s) 602, 1308
SacI GAGCTC 1 cut(s) 1459
SaqAI TTAA 3 cut(s) 896, 1656, 2057
SatI GCNGC 5 cut(s) 445, 1321, 1330, 2007, 2010
Sau3AI GATC 9 cut(s) 198, 241, 260, 310, 658, 984, 1050, 1300, 1898
Sau96I GGNCC 4 cut(s) 653, 773, 1039, 1295
ScaI AGTACT 2 cut(s) 1564, 1911
SchI GAGTC 6 cut(s) 125, 200, 1485, 2196, 2252, 2295
ScrFI CCNGG 1 cut(s) 1791
SduI GDGCHC 2 cut(s) 1459, 1558
SfcI CTRYAG 3 cut(s) 153, 915, 1734
Sfr274I CTCGAG 1 cut(s) 2260
SfuI TTCGAA 1 cut(s) 1087
SinI GGWCC 4 cut(s) 653, 773, 1039, 1295
SlaI CTCGAG 1 cut(s) 2260
SmiMI CAYNNNNRTG 2 cut(s) 602, 1308
SmlI CTYRAG 3 cut(s) 600, 1982, 2260
SmoI CTYRAG 3 cut(s) 600, 1982, 2260
SsiI CCGC 5 cut(s) 939, 1551, 1587, 2006, 2012
SspI AATATT 3 cut(s) 1279, 1572, 1606
SspMI CTAG 4 cut(s) 335, 359, 2079, 2145
SstI GAGCTC 1 cut(s) 1459
StyD4I CCNGG 1 cut(s) 1789
StyI CCWWGG 6 cut(s) 225, 784, 966, 1126, 1167, 1814
TaaI ACNGT 6 cut(s) 1140, 1148, 1155, 1729, 1909, 2305
TaiI ACGT 3 cut(s) 162, 206, 2231
TatI WGTACW 3 cut(s) 1270, 1562, 1909
TauI GCSGC 1 cut(s) 2009
TfiI GAWTC 4 cut(s) 1221, 1526, 1579, 1667
Tru1I TTAA 3 cut(s) 896, 1656, 2057
Tru9I TTAA 3 cut(s) 896, 1656, 2057
TscAI CASTG 5 cut(s) 130, 733, 1738, 2315, 2332
TseFI GTSAC 4 cut(s) 134, 167, 1534, 1729
TseI GCWGC 4 cut(s) 444, 1320, 1329, 2009
Tsp45I GTSAC 4 cut(s) 134, 167, 1534, 1729
TspDTI ATGAA 9 cut(s) 33, 386, 415, 530, 729, 1227, 1512, 1974, 2130
TspGWI ACGGA 1 cut(s) 723
TspRI CASTG 5 cut(s) 130, 733, 1738, 2315, 2332
Van91I CCANNNNNTGG 2 cut(s) 1189, 1258
VpaK11BI GGWCC 4 cut(s) 653, 773, 1039, 1295
XapI RAATTY 4 cut(s) 764, 846, 1487, 1615
XceI RCATGY 3 cut(s) 29, 320, 1103
XhoI CTCGAG 1 cut(s) 2260
XmiI GTMKAC 1 cut(s) 1724
XspI CTAG 4 cut(s) 335, 359, 2079, 2145
ZraI GACGTC 1 cut(s) 160
ZrmI AGTACT 2 cut(s) 1564, 1911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.