Rroxscaffold_3G00253650

Auxin canalisation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011081
Physical Location & Seq
Reverse (-)
47731607 .. 47735726
4120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00253650.1

Sequence Viewer

Length: 1197 bp
ATGGAAGACAGTTATCACAGTTTAGCCTCGAAGATTAGTAATACAGGTGGTTCTTTGCATGGGCCAGAGATGTTACAGGAAGCAGATGACATGAAAATCGCTCCATATATGGCTGGAATTCCTCAACCACAGACACCGCAGGAGCCTATGGAATTCTTGTCAAGGTCATGGAGTATCTCTGCTTCAGAAATCTCGAAGGCTCTCGCTAAAAAACAGAAGCAATCTAACATTCCCAAGAGTCCTGACACAGTCCTAGAATCAATTCCTTTACCACAACTGCGAGGCAAGGTCAGGAAATCTGGTACTAAACAGAGTCGGAGAACCGGGTCAATTGGAAAATGGTTCAACAATGAGTTCACTAATAGCACAGTGAAGAAGAAAGACAAGGAACGGATGGAAAGGGCGCGAGTGCATTCTGCGGTCTCCATTGCAGGAGTTGCTGCAGCCTTGGCTGCTATAATGGCAGCAGAAAACACCAGTGGCCCTCCCTCGAAAATGGCCATGTCTTTGGCATTGGCTACAGAATTGCTGGCATCACATTCCATTGAAATGGCAGAGTCGGAAGGAGCTGATCGCAACCGTCTGGCATCTACTGTCAGATCAGCCCTTGACATTCAGAGTCCTGGTGATCTTATGACCCTTACAGCAGCAGCTGCAACAGAAAGTTTTTCTATAGCGTTGCGAGGAGAAGCAGCACTGAAAGCAAGATTACCGAAAGAGCCGAAAAAGAATGCGGCCATTAGTCCCTATGATAGGGGAATGGCAGAAATTACTTCACCTTCGGCTTTTCATAGTCATATGGAGGAGCAGGATGGTCCCTGTGATGGAGATTTACTGCAACTTATGGGAAAAGGTATTCAACAATGGAAGAGTGTCTCTGTCTACATTAAAAAATCTCAGGTCATGATTAAACTCAAACGTAAACACGTTGGGGGAGCCTTTGCCAAGAAGAGTAAAAGTGTTGTTTATGGAGTCTGTGATGAGAATTCTGCTTGGCCATACATGATAGAAAGGGATATTCCGGAAGAGGTTTATTTTGGCCTCAAAACTGGACAGGGTTTAATGGAATTCAAGTGCAGGAGTAAGGCCCATAAACAGCAATGGGTTGATGGGATCAAAAATCTTCTTCGTCGAGTCAGCTCTCTTGATGAAGCCGAGCGCTCTATCGGACTTTTAAGCGTCAGCAATAGTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_canalis PH_2

Protein Analysis

398

Amino Acids

43.41

Weight (kDa)

9.36

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_canalis PF05703 43 - 75 2.2e-11 Auxin canalisation
Auxin_canalis PF05703 98 - 252 1.3e-43 Auxin canalisation
PH_2 PF08458 277 - 379 2.3e-34 Plant pleckstrin homology-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011081)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17350 AT5G47440
fragaria_vesca FvH4_5g19120 FvH4_5g19120
malus_domestica MD06G1056800.v1.1
prunus_persica Prupe.5G067500_v2.0.a1 Prupe.5G067500_v2.0.a1 Prupe.5G067500_v2.0.a1
pyrus_communis pycom06g04740
rosa_chinensis RchiOBHm_Chr7g0203601
rosa_laevigata RLG00000003515
rosa_multiflora Rmu_sc0010675.1_g000003
rosa_roxburghii Rroxscaffold_3G00253650
rosa_rugosa Rorug07G0077200 Rorug07G0077300
rosa_samantha Rh7AG207000 Rh7BG206500 Rh7CG219600 Rh7DG214200
rosa_wichuraiana Rw7G018060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 882
AccII CGCG 1 cut(s) 406
AccIII TCCGGA 1 cut(s) 1021
AciI CCGC 3 cut(s) 137, 419, 734
AclWI GGATC 1 cut(s) 1121
AcoI YGGCCR 3 cut(s) 498, 735, 995
AcsI RAATTY 4 cut(s) 117, 152, 985, 1067
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 1 cut(s) 304
AfeI AGCGCT 1 cut(s) 1160
AfiI CCNNNNNNNGG 2 cut(s) 753, 824
AflIII ACRYGT 1 cut(s) 925
AgsI TTSAA 4 cut(s) 346, 548, 860, 1072
AjnI CCWGG 1 cut(s) 622
AluBI AGCT 3 cut(s) 569, 653, 1140
AluI AGCT 3 cut(s) 569, 653, 1140
Alw26I GTCTC 2 cut(s) 427, 880
AlwI GGATC 1 cut(s) 1121
AlwNI CAGNNNCTG 1 cut(s) 653
Aor13HI TCCGGA 1 cut(s) 1021
Aor51HI AGCGCT 1 cut(s) 1160
AoxI GGCC 7 cut(s) 62, 481, 498, 735, 995, 1039, 1086
ApeKI GCWGC 8 cut(s) 440, 443, 452, 464, 647, 650, 653, 692
ApoI RAATTY 4 cut(s) 117, 152, 985, 1067
Asp700I GAANNNNTTC 1 cut(s) 261
AspLEI GCGC 2 cut(s) 406, 1161
AspS9I GGNCC 4 cut(s) 62, 482, 815, 1087
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 2 cut(s) 638, 768
AvaII GGWCC 1 cut(s) 815
BalI TGGCCA 2 cut(s) 500, 997
BarI GAAGNNNNNNTAC 2 cut(s) 166, 198
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 8 cut(s) 427, 439, 455, 476, 640, 659, 662, 704
BccI CCATC 4 cut(s) 388, 806, 818, 1103
BciT130I CCWGG 1 cut(s) 624
BcnI CCSGG 1 cut(s) 325
BcoDI GTCTC 2 cut(s) 427, 880
BfaI CTAG 1 cut(s) 254
BfmI CTRYAG 3 cut(s) 441, 519, 672
BfoI RGCGCY 1 cut(s) 1162
BisI GCNGC 9 cut(s) 441, 444, 453, 465, 648, 651, 654, 693, 735
BlsI GCNGC 9 cut(s) 442, 445, 454, 466, 649, 652, 655, 694, 736
Bme1390I CCNGG 2 cut(s) 325, 624
Bme18I GGWCC 1 cut(s) 815
BmgT120I GGNCC 4 cut(s) 62, 482, 815, 1087
BmiI GGNNCC 3 cut(s) 144, 817, 937
BmrFI CCNGG 2 cut(s) 325, 624
BmsI GCATC 2 cut(s) 542, 596
BpiI GAAGAC 1 cut(s) 12
BpuMI CCSGG 1 cut(s) 325
BsaI GGTCTC 1 cut(s) 427
BsaJI CCNNGG 1 cut(s) 447
BsaWI WCCGGW 1 cut(s) 1021
Bsc4I CCNNNNNNNGG 2 cut(s) 753, 824
Bse1I ACTGG 2 cut(s) 477, 1054
Bse3DI GCAATG 2 cut(s) 426, 1106
BseAI TCCGGA 1 cut(s) 1021
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 1 cut(s) 447
BseGI GGATG 2 cut(s) 399, 817
BseLI CCNNNNNNNGG 2 cut(s) 753, 824
BseMI GCAATG 2 cut(s) 426, 1106
BseMII CTCAG 1 cut(s) 911
BseNI ACTGG 2 cut(s) 477, 1054
BseRI GAGGAG 2 cut(s) 699, 818
BseXI GCAGC 8 cut(s) 427, 439, 455, 476, 640, 659, 662, 704
BsgI GTGCAG 1 cut(s) 1096
Bsh1236I CGCG 1 cut(s) 406
BshFI GGCC 7 cut(s) 64, 483, 500, 737, 997, 1041, 1088
BsiSI CCGG 2 cut(s) 324, 1022
BslFI GGGAC 2 cut(s) 729, 801
BslI CCNNNNNNNGG 2 cut(s) 753, 824
BsmAI GTCTC 2 cut(s) 427, 880
BsmFI GGGAC 2 cut(s) 729, 801
BsmI GAATGC 2 cut(s) 412, 736
BsnI GGCC 7 cut(s) 64, 483, 500, 737, 997, 1041, 1088
Bso31I GGTCTC 1 cut(s) 427
Bsp13I TCCGGA 1 cut(s) 1021
Bsp143I GATC 4 cut(s) 571, 599, 628, 1113
BspACI CCGC 3 cut(s) 137, 419, 734
BspANI GGCC 7 cut(s) 64, 483, 500, 737, 997, 1041, 1088
BspCNI CTCAG 1 cut(s) 910
BspEI TCCGGA 1 cut(s) 1021
BspFNI CGCG 1 cut(s) 406
BspHI TCATGA 1 cut(s) 903
BspLI GGNNCC 3 cut(s) 144, 817, 937
BspMAI CTGCAG 1 cut(s) 445
BspPI GGATC 1 cut(s) 1121
BspTNI GGTCTC 1 cut(s) 427
BsrDI GCAATG 2 cut(s) 426, 1106
BsrI ACTGG 2 cut(s) 477, 1054
BssECI CCNNGG 1 cut(s) 447
BssMI GATC 4 cut(s) 571, 599, 628, 1113
BssT1I CCWWGG 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 6 cut(s) 11, 20, 250, 370, 581, 595
Bst6I CTCTTC 3 cut(s) 863, 944, 1020
BstAPI GCANNNNNTGC 2 cut(s) 437, 653
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 1 cut(s) 897
BstENI CCTNNNNNAGG 1 cut(s) 751
BstF5I GGATG 2 cut(s) 399, 817
BstFNI CGCG 1 cut(s) 406
BstH2I RGCGCY 1 cut(s) 1162
BstHHI GCGC 2 cut(s) 406, 1161
BstKTI GATC 4 cut(s) 574, 602, 631, 1116
BstMAI GTCTC 2 cut(s) 427, 880
BstMBI GATC 4 cut(s) 571, 599, 628, 1113
BstMWI GCNNNNNNNGC 6 cut(s) 437, 449, 452, 461, 653, 701
BstNI CCWGG 1 cut(s) 624
BstSCI CCNGG 2 cut(s) 323, 622
BstSFI CTRYAG 3 cut(s) 441, 519, 672
BstUI CGCG 1 cut(s) 406
BstV1I GCAGC 8 cut(s) 427, 439, 455, 476, 640, 659, 662, 704
BstV2I GAAGAC 1 cut(s) 12
BstXI CCANNNNNNTGG 2 cut(s) 508, 550
BsuRI GGCC 7 cut(s) 64, 483, 500, 737, 997, 1041, 1088
BtsCI GGATG 2 cut(s) 399, 817
BtsIMutI CAGTG 3 cut(s) 375, 484, 695
Cac8I GCNNGC 1 cut(s) 531
CaiI CAGNNNCTG 1 cut(s) 653
CciI TCATGA 1 cut(s) 903
CfoI GCGC 2 cut(s) 406, 1161
Cfr13I GGNCC 4 cut(s) 62, 482, 815, 1087
CseI GACGC 1 cut(s) 1168
Csp6I GTAC 1 cut(s) 303
CviAII CATG 6 cut(s) 59, 91, 168, 502, 904, 1003
CviQI GTAC 1 cut(s) 303
DdeI CTNAG 1 cut(s) 897
DpnI GATC 4 cut(s) 573, 601, 630, 1115
DpnII GATC 4 cut(s) 571, 599, 628, 1113
EaeI YGGCCR 3 cut(s) 498, 735, 995
Eam1104I CTCTTC 3 cut(s) 863, 944, 1020
EarI CTCTTC 3 cut(s) 863, 944, 1020
Eco130I CCWWGG 1 cut(s) 447
Eco31I GGTCTC 1 cut(s) 427
Eco47I GGWCC 1 cut(s) 815
Eco47III AGCGCT 1 cut(s) 1160
Eco57I CTGAAG 1 cut(s) 168
EcoNI CCTNNNNNAGG 1 cut(s) 751
EcoRI GAATTC 4 cut(s) 117, 152, 985, 1067
EcoRII CCWGG 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 6 cut(s) 62, 94, 171, 505, 907, 1006
FaqI GGGAC 2 cut(s) 729, 801
FatI CATG 6 cut(s) 58, 90, 167, 501, 903, 1002
FauNDI CATATG 1 cut(s) 798
FblI GTMKAC 1 cut(s) 882
Fnu4HI GCNGC 9 cut(s) 441, 444, 453, 465, 648, 651, 654, 693, 735
FokI GGATG 2 cut(s) 406, 824
Fsp4HI GCNGC 9 cut(s) 441, 444, 453, 465, 648, 651, 654, 693, 735
FspBI CTAG 1 cut(s) 254
GlaI GCGC 2 cut(s) 405, 1160
GluI GCNGC 9 cut(s) 441, 444, 453, 465, 648, 651, 654, 693, 735
HaeII RGCGCY 1 cut(s) 1162
HaeIII GGCC 7 cut(s) 64, 483, 500, 737, 997, 1041, 1088
HapII CCGG 2 cut(s) 324, 1022
HgaI GACGC 1 cut(s) 1168
HhaI GCGC 2 cut(s) 406, 1161
Hin1II CATG 6 cut(s) 62, 94, 171, 505, 907, 1006
Hin6I GCGC 2 cut(s) 404, 1159
HinP1I GCGC 2 cut(s) 404, 1159
HinfI GANTC 7 cut(s) 238, 257, 313, 557, 619, 972, 1134
HpaII CCGG 2 cut(s) 324, 1022
HphI GGTGA 2 cut(s) 638, 768
Hpy166II GTNNAC 3 cut(s) 357, 883, 923
Hpy188I TCNGA 6 cut(s) 187, 318, 562, 599, 618, 1169
Hpy188III TCNNGA 6 cut(s) 193, 242, 292, 904, 1022, 1145
Hpy8I GTNNAC 3 cut(s) 357, 883, 923
Hpy99I CGWCG 1 cut(s) 1134
HpyAV CCTTC 3 cut(s) 190, 557, 789
HpyCH4III ACNGT 6 cut(s) 11, 20, 250, 370, 581, 595
HpyCH4IV ACGT 2 cut(s) 919, 927
HpyCH4V TGCA 7 cut(s) 58, 412, 431, 443, 656, 838, 1077
HpyF10VI GCNNNNNNNGC 6 cut(s) 437, 449, 452, 461, 653, 701
HpyF3I CTNAG 1 cut(s) 897
HpySE526I ACGT 2 cut(s) 919, 927
Hsp92II CATG 6 cut(s) 62, 94, 171, 505, 907, 1006
HspAI GCGC 2 cut(s) 404, 1159
Kpn2I TCCGGA 1 cut(s) 1021
Kzo9I GATC 4 cut(s) 571, 599, 628, 1113
LmnI GCTCC 5 cut(s) 106, 142, 566, 805, 935
Lsp1109I GCAGC 8 cut(s) 427, 439, 455, 476, 640, 659, 662, 704
LweI GCATC 2 cut(s) 542, 596
MaeI CTAG 1 cut(s) 254
MaeII ACGT 2 cut(s) 919, 927
MaeIII GTNAC 1 cut(s) 72
MalI GATC 4 cut(s) 573, 601, 630, 1115
MboI GATC 4 cut(s) 571, 599, 628, 1113
MboII GAAGA 9 cut(s) 17, 43, 385, 388, 880, 961, 1037, 1115, 1118
MfeI CAATTG 1 cut(s) 330
MlsI TGGCCA 2 cut(s) 500, 997
MluCI AATT 8 cut(s) 117, 152, 261, 330, 524, 768, 985, 1067
MluNI TGGCCA 2 cut(s) 500, 997
MlyI GAGTC 6 cut(s) 247, 322, 566, 628, 981, 1143
MmeI TCCRAC 2 cut(s) 296, 540
MnlI CCTC 9 cut(s) 37, 132, 275, 495, 499, 677, 796, 1021, 1052
Mox20I TGGCCA 2 cut(s) 500, 997
MroI TCCGGA 1 cut(s) 1021
MroXI GAANNNNTTC 1 cut(s) 261
MscI TGGCCA 2 cut(s) 500, 997
MseI TTAA 4 cut(s) 888, 909, 1061, 1175
MslI CAYNNNNRTG 1 cut(s) 548
Msp20I TGGCCA 2 cut(s) 500, 997
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 2 cut(s) 324, 1022
MspR9I CCNGG 2 cut(s) 325, 624
MunI CAATTG 1 cut(s) 330
Mva1269I GAATGC 2 cut(s) 412, 736
MvaI CCWGG 1 cut(s) 624
MvnI CGCG 1 cut(s) 406
MwoI GCNNNNNNNGC 6 cut(s) 437, 449, 452, 461, 653, 701
NciI CCSGG 1 cut(s) 325
NdeI CATATG 1 cut(s) 798
NdeII GATC 4 cut(s) 571, 599, 628, 1113
NlaIII CATG 6 cut(s) 62, 94, 171, 505, 907, 1006
NlaIV GGNNCC 3 cut(s) 144, 817, 937
NmeAIII GCCGAG 1 cut(s) 1180
PagI TCATGA 1 cut(s) 903
PctI GAATGC 2 cut(s) 412, 736
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 1 cut(s) 257
PflFI GACNNNGTC 1 cut(s) 248
PkrI GCNGC 9 cut(s) 442, 445, 454, 466, 649, 652, 655, 694, 736
PleI GAGTC 6 cut(s) 246, 321, 565, 627, 980, 1142
PpsI GAGTC 6 cut(s) 246, 321, 565, 627, 980, 1142
Psp6I CCWGG 1 cut(s) 622
PspGI CCWGG 1 cut(s) 622
PspN4I GGNNCC 3 cut(s) 144, 817, 937
PspPI GGNCC 4 cut(s) 62, 482, 815, 1087
PstI CTGCAG 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 653
PsyI GACNNNGTC 1 cut(s) 248
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 1 cut(s) 304
RsaNI GTAC 1 cut(s) 303
RseI CAYNNNNRTG 1 cut(s) 548
SaqAI TTAA 4 cut(s) 888, 909, 1061, 1175
SatI GCNGC 9 cut(s) 441, 444, 453, 465, 648, 651, 654, 693, 735
Sau3AI GATC 4 cut(s) 571, 599, 628, 1113
Sau96I GGNCC 4 cut(s) 62, 482, 815, 1087
SchI GAGTC 6 cut(s) 247, 322, 566, 628, 981, 1143
ScrFI CCNGG 2 cut(s) 325, 624
SfaNI GCATC 2 cut(s) 542, 596
SfcI CTRYAG 3 cut(s) 441, 519, 672
SinI GGWCC 1 cut(s) 815
SmiMI CAYNNNNRTG 1 cut(s) 548
Sse9I AATT 8 cut(s) 117, 152, 261, 330, 524, 768, 985, 1067
SsiI CCGC 3 cut(s) 137, 419, 734
SspMI CTAG 1 cut(s) 254
StyD4I CCNGG 2 cut(s) 323, 622
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 6 cut(s) 11, 20, 250, 370, 581, 595
TaiI ACGT 2 cut(s) 922, 930
TaqI TCGA 4 cut(s) 29, 194, 491, 1132
TasI AATT 8 cut(s) 117, 152, 261, 330, 524, 768, 985, 1067
TauI GCSGC 1 cut(s) 737
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 4 cut(s) 888, 909, 1061, 1175
Tru9I TTAA 4 cut(s) 888, 909, 1061, 1175
TscAI CASTG 3 cut(s) 375, 484, 702
TseI GCWGC 8 cut(s) 440, 443, 452, 464, 647, 650, 653, 692
TspDTI ATGAA 3 cut(s) 107, 779, 1164
TspGWI ACGGA 1 cut(s) 406
TspRI CASTG 3 cut(s) 375, 484, 702
Tth111I GACNNNGTC 1 cut(s) 248
VpaK11BI GGWCC 1 cut(s) 815
XagI CCTNNNNNAGG 1 cut(s) 751
XapI RAATTY 4 cut(s) 117, 152, 985, 1067
XmiI GTMKAC 1 cut(s) 882
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.