Rroxscaffold_3G00273000

WW domain binding protein 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008220
Physical Location & Seq
Forward (+)
64919546 .. 64931379
11834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00273000.1

Sequence Viewer

Length: 1530 bp
ATGAAGACGACCAAGGGAGGCAAGGTCATGAACCCTACGGACGCTTATCGTAAGGAGCTCCGCAAAAAGGAATTGAAAAGGAACAAGAAAGAAAGAAAGAAGGTGAGGGAGGTGGGGATCTTGAAGAAGGATCCCGAGACCCTGAAGGAGCAGATTGATAAGTTGAATAAGCAGAAGGCCGATGGTGCTCTGGACAAAGCAAGAAAACACAAGAAGAGGCAACTAGAGGATACACTTAATCTTGTTATGAAGAAGAGGAAGGAAGTTGAAGAGAAGGCAAAGGAGAAGGGTGAGACCCCGGTTATGTTCAGCCATTTGGGACCACCTAAAAGACGAACAACCGCTGAAGAAGAAGAAAGAGCAAAGCATCCAAAGCCTGAGGATTCTGTTTACTACCACCCAACCTTGAATCCTACTGGGGCCCCACCACCTGGAAAGCCTCCAATGTTTCAATCATCAATAGGACCAAGAATTCCTTTATCTGCTGCTTCATCAAGTGGGGCTGCATCATCCACTGAGGATGGAATTTTGGCTGATCCTCTGCTCAATTCTGGTGATGGCTCCGTTCTACCGGAGTCTTTACCTCTACCTCCTCCGCCTCCATTGCCGCCCAACCCTGCTGCTGCAAACTCAGGTATCTCACTGCCACCACCTCCACCTCCTCCTCCTGGACCCCTGCCTCCTAAAGAGGAGATTGCTAATCGCACTCTACTTCCCCCTCCTCCACCTCTTCACCAGTCTGCACAGCCACCTCCACCTGGTATCAGTGGAAGCGAGAGGGAGAACAATCAATCTCCATTTTCTGATCAACCACCTGCCAAGGATCTCACACAGGTACTTCCTCCCCCTCCTCCCCCCTCCTGGGATCTTACTAAAATGGTTCCACCACCACCTCCTCCAAGACAACAACCTCCAGTGCCTGGACCCACCCTGATTCCAAATATACATCCCGATGTGATACCCCCAGGTATCTCTCGTTTTCCTCCACCGCCACCTCCACCAGATATGCGGCCACCATTATCTGCACCTGGACTTCCCAGTCGACCAGGTCCACCAGGCATGATGGTCCCAATGATTCCCAGGCCACCATATGGACCTCCCCCTGGACCTCCCCCAATGATGAGACCACCACTTCCACCTGGCCCCCTCCTACATTTCAAGAAGATGACCATGCAGCCTACGGCCACACTCCAGAACTTACAGCCATGCAGTAGCTTCATGCGCTCTTTACTAACTACTGTTGTGAAACCAGACAAAATCCAACATACGTTGTCACTAAATGTTGTCGATCCTGCATTTGTACACTTGAGACCGGAGCCTTCCTTGATCATGCCGAAGTTTAAATGCGGAACGGTGGCTAATCATCCTATCGAATCATCCGAGTCGACTATTGAATTAAAGTCTAAGCATTCCACTACTTGTAGTTCGTGCTCACCGTATAGTTCCTCATCAAAAATCTCAGCTGTCCATTCTTCTCTAACCGCTCGTCTTGAGGTCCATAAGAAGTTAGGCAAGCTACTTGGGAGGTAA

Protein Analysis

509

Amino Acids

55.1

Weight (kDa)

9.82

Isoelectric Point (pI)

67.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Wbp11 PF09429 7 - 84 1.7e-22 WW domain binding protein 11
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 823
AasI GACNNNNNNGTC 1 cut(s) 1038
Acc36I ACCTGC 1 cut(s) 823
AccB7I CCANNNNNTGG 3 cut(s) 431, 920, 1091
AccBSI CCGCTC 1 cut(s) 1484
AccI GTMKAC 2 cut(s) 1042, 1385
AciI CCGC 8 cut(s) 61, 342, 596, 608, 989, 1009, 1347, 1482
AclWI GGATC 7 cut(s) 125, 125, 138, 530, 831, 873, 1283
AcoI YGGCCR 2 cut(s) 1010, 1182
AcsI RAATTY 2 cut(s) 471, 525
AcuI CTGAAG 2 cut(s) 164, 366
AfaI GTAC 2 cut(s) 837, 1302
AfiI CCNNNNNNNGG 9 cut(s) 67, 431, 668, 758, 861, 862, 920, 1091, 1103
AgsI TTSAA 8 cut(s) 76, 124, 166, 269, 409, 452, 1159, 1394
AluBI AGCT 4 cut(s) 58, 1215, 1463, 1516
AluI AGCT 4 cut(s) 58, 1215, 1463, 1516
Alw21I GWGCWC 3 cut(s) 60, 190, 1433
Alw26I GTCTC 4 cut(s) 131, 287, 1117, 1303
AlwI GGATC 7 cut(s) 125, 125, 138, 530, 831, 873, 1283
AlwNI CAGNNNCTG 1 cut(s) 920
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 6 cut(s) 177, 420, 1010, 1082, 1141, 1182
ApaI GGGCCC 1 cut(s) 424
ApeKI GCWGC 5 cut(s) 485, 503, 620, 623, 1174
ApoI RAATTY 2 cut(s) 471, 525
AspLEI GCGC 1 cut(s) 1224
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 5 cut(s) 115, 302, 566, 725, 1425
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 9 cut(s) 320, 464, 671, 923, 1049, 1066, 1094, 1106, 1495
AxyI CCTNAGG 1 cut(s) 378
BaeGI GKGCMC 1 cut(s) 424
BaeI ACNNNNGTAYC 2 cut(s) 619, 652
BamHI GGATCC 1 cut(s) 130
BanII GRGCYC 2 cut(s) 60, 424
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 3 cut(s) 60, 190, 1433
BbvI GCAGC 5 cut(s) 472, 490, 607, 610, 1186
BccI CCATC 4 cut(s) 176, 515, 551, 1057
BceAI ACGGC 1 cut(s) 1197
BciVI GTATCC 1 cut(s) 223
BclI TGATCA 2 cut(s) 805, 1326
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 4 cut(s) 131, 287, 1117, 1303
BfaI CTAG 1 cut(s) 224
BfuAI ACCTGC 1 cut(s) 823
BfuI GTATCC 1 cut(s) 223
BisI GCNGC 7 cut(s) 486, 504, 608, 621, 624, 1010, 1175
BlsI GCNGC 7 cut(s) 487, 505, 609, 622, 625, 1011, 1176
Bme18I GGWCC 9 cut(s) 320, 464, 671, 923, 1049, 1066, 1094, 1106, 1495
BmeT110I CYCGRG 1 cut(s) 134
BmrI ACTGGG 2 cut(s) 426, 1032
BmsI GCATC 2 cut(s) 376, 515
BmuI ACTGGG 2 cut(s) 426, 1032
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 2 cut(s) 897, 1175
BpuEI CTTGAG 2 cut(s) 1327, 1511
BpuMI CCSGG 1 cut(s) 299
BsaI GGTCTC 4 cut(s) 131, 287, 1117, 1303
BsaJI CCNNGG 7 cut(s) 12, 297, 819, 861, 964, 1079, 1102
BsaWI WCCGGW 2 cut(s) 571, 1312
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 9 cut(s) 67, 431, 668, 758, 861, 862, 920, 1091, 1103
Bse1I ACTGG 4 cut(s) 421, 736, 914, 1038
Bse21I CCTNAGG 1 cut(s) 378
Bse3DI GCAATG 1 cut(s) 602
BseDI CCNNGG 7 cut(s) 12, 297, 819, 861, 964, 1079, 1102
BseGI GGATG 6 cut(s) 367, 509, 526, 946, 1363, 1376
BseLI CCNNNNNNNGG 9 cut(s) 67, 431, 668, 758, 861, 862, 920, 1091, 1103
BseMI GCAATG 1 cut(s) 602
BseMII CTCAG 4 cut(s) 369, 507, 645, 1473
BseNI ACTGG 4 cut(s) 421, 736, 914, 1038
BseRI GAGGAG 7 cut(s) 582, 651, 654, 704, 711, 840, 885
BseSI GKGCMC 1 cut(s) 424
BseXI GCAGC 5 cut(s) 472, 490, 607, 610, 1186
BsgI GTGCAG 2 cut(s) 726, 1008
BshFI GGCC 6 cut(s) 179, 422, 1012, 1084, 1143, 1184
BsiHKAI GWGCWC 3 cut(s) 60, 190, 1433
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 3 cut(s) 299, 572, 1313
BslFI GGGAC 2 cut(s) 333, 1052
BslI CCNNNNNNNGG 9 cut(s) 67, 431, 668, 758, 861, 862, 920, 1091, 1103
BsmAI GTCTC 4 cut(s) 131, 287, 1117, 1303
BsmFI GGGAC 2 cut(s) 333, 1052
BsmI GAATGC 1 cut(s) 1408
BsnI GGCC 6 cut(s) 179, 422, 1012, 1084, 1143, 1184
Bso31I GGTCTC 4 cut(s) 131, 287, 1117, 1303
BsoBI CYCGRG 1 cut(s) 134
Bsp120I GGGCCC 1 cut(s) 420
Bsp1286I GDGCHC 4 cut(s) 60, 190, 424, 1433
Bsp1407I TGTACA 1 cut(s) 1300
Bsp143I GATC 8 cut(s) 117, 130, 535, 805, 823, 865, 1288, 1326
BspACI CCGC 8 cut(s) 61, 342, 596, 608, 989, 1009, 1347, 1482
BspANI GGCC 6 cut(s) 179, 422, 1012, 1084, 1143, 1184
BspCNI CTCAG 4 cut(s) 370, 508, 644, 1472
BspHI TCATGA 1 cut(s) 27
BspMI ACCTGC 1 cut(s) 823
BspPI GGATC 7 cut(s) 125, 125, 138, 530, 831, 873, 1283
BspTNI GGTCTC 4 cut(s) 131, 287, 1117, 1303
BsrBI CCGCTC 1 cut(s) 1484
BsrDI GCAATG 1 cut(s) 602
BsrGI TGTACA 1 cut(s) 1300
BsrI ACTGG 4 cut(s) 421, 736, 914, 1038
BssECI CCNNGG 7 cut(s) 12, 297, 819, 861, 964, 1079, 1102
BssMI GATC 8 cut(s) 117, 130, 535, 805, 823, 865, 1288, 1326
BssT1I CCWWGG 2 cut(s) 12, 819
Bst4CI ACNGT 3 cut(s) 1240, 1354, 1437
Bst6I CTCTTC 4 cut(s) 209, 248, 264, 735
BstAUI TGTACA 1 cut(s) 1300
BstC8I GCNNGC 1 cut(s) 1514
BstDEI CTNAG 5 cut(s) 378, 516, 631, 1404, 1459
BstF5I GGATG 6 cut(s) 367, 509, 526, 946, 1363, 1376
BstHHI GCGC 1 cut(s) 1224
BstKTI GATC 8 cut(s) 120, 133, 538, 808, 826, 868, 1291, 1329
BstMAI GTCTC 4 cut(s) 131, 287, 1117, 1303
BstMBI GATC 8 cut(s) 117, 130, 535, 805, 823, 865, 1288, 1326
BstMWI GCNNNNNNNGC 4 cut(s) 185, 373, 604, 1221
BstSLI GKGCMC 1 cut(s) 424
BstV1I GCAGC 5 cut(s) 472, 490, 607, 610, 1186
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 4 cut(s) 117, 130, 823, 865
BstYI RGATCY 4 cut(s) 117, 130, 823, 865
Bsu36I CCTNAGG 1 cut(s) 378
BsuI GTATCC 1 cut(s) 223
BsuRI GGCC 6 cut(s) 179, 422, 1012, 1084, 1143, 1184
BtsCI GGATG 6 cut(s) 367, 509, 526, 946, 1363, 1376
BtsI GCAGTG 1 cut(s) 641
BtsIMutI CAGTG 4 cut(s) 513, 641, 772, 921
BveI ACCTGC 1 cut(s) 823
Cac8I GCNNGC 1 cut(s) 1514
CaiI CAGNNNCTG 1 cut(s) 920
CciI TCATGA 1 cut(s) 27
CfoI GCGC 1 cut(s) 1224
CseI GACGC 1 cut(s) 50
CsiI ACCWGGT 2 cut(s) 757, 1045
Csp6I GTAC 2 cut(s) 836, 1301
CviAII CATG 6 cut(s) 28, 1060, 1171, 1206, 1219, 1330
CviQI GTAC 2 cut(s) 836, 1301
DdeI CTNAG 5 cut(s) 378, 516, 631, 1404, 1459
DpnI GATC 8 cut(s) 119, 132, 537, 807, 825, 867, 1290, 1328
DpnII GATC 8 cut(s) 117, 130, 535, 805, 823, 865, 1288, 1326
DraI TTTAAA 1 cut(s) 1342
DrdI GACNNNNNNGTC 1 cut(s) 1038
DseDI GACNNNNNNGTC 1 cut(s) 1038
EaeI YGGCCR 2 cut(s) 1010, 1182
Eam1104I CTCTTC 4 cut(s) 209, 248, 264, 735
EarI CTCTTC 4 cut(s) 209, 248, 264, 735
EciI GGCGGA 1 cut(s) 585
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 2 cut(s) 12, 819
Eco24I GRGCYC 2 cut(s) 60, 424
Eco31I GGTCTC 4 cut(s) 131, 287, 1117, 1303
Eco47I GGWCC 9 cut(s) 320, 464, 671, 923, 1049, 1066, 1094, 1106, 1495
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 2 cut(s) 164, 366
Eco81I CCTNAGG 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 58
EcoO109I RGGNCCY 2 cut(s) 420, 421
EcoRI GAATTC 1 cut(s) 471
EcoT14I CCWWGG 2 cut(s) 12, 819
EcoT38I GRGCYC 2 cut(s) 60, 424
ErhI CCWWGG 2 cut(s) 12, 819
FaeI CATG 6 cut(s) 31, 1063, 1174, 1209, 1222, 1333
FalI AAGNNNNNCTT 2 cut(s) 460, 492
FaqI GGGAC 2 cut(s) 333, 1052
FatI CATG 6 cut(s) 27, 1059, 1170, 1205, 1218, 1329
FauNDI CATATG 1 cut(s) 1090
FbaI TGATCA 2 cut(s) 805, 1326
FblI GTMKAC 2 cut(s) 1042, 1385
Fnu4HI GCNGC 7 cut(s) 486, 504, 608, 621, 624, 1010, 1175
FokI GGATG 6 cut(s) 354, 496, 533, 933, 1350, 1363
FriOI GRGCYC 2 cut(s) 60, 424
Fsp4HI GCNGC 7 cut(s) 486, 504, 608, 621, 624, 1010, 1175
FspBI CTAG 1 cut(s) 224
GlaI GCGC 1 cut(s) 1223
GluI GCNGC 7 cut(s) 486, 504, 608, 621, 624, 1010, 1175
GsuI CTGGAG 2 cut(s) 897, 1175
HaeIII GGCC 6 cut(s) 179, 422, 1012, 1084, 1143, 1184
HapII CCGG 3 cut(s) 299, 572, 1313
HgaI GACGC 1 cut(s) 50
HhaI GCGC 1 cut(s) 1224
Hin1II CATG 6 cut(s) 31, 1063, 1174, 1209, 1222, 1333
Hin6I GCGC 1 cut(s) 1222
HinP1I GCGC 1 cut(s) 1222
HincII GTYRAC 2 cut(s) 1043, 1386
HindII GTYRAC 2 cut(s) 1043, 1386
HinfI GANTC 7 cut(s) 383, 409, 575, 934, 1075, 1373, 1382
HpaII CCGG 3 cut(s) 299, 572, 1313
HphI GGTGA 5 cut(s) 115, 302, 566, 725, 1425
Hpy166II GTNNAC 5 cut(s) 391, 1043, 1052, 1303, 1386
Hpy188I TCNGA 2 cut(s) 805, 1381
Hpy188III TCNNGA 8 cut(s) 28, 121, 134, 191, 950, 1159, 1192, 1490
Hpy8I GTNNAC 5 cut(s) 391, 1043, 1052, 1303, 1386
HpyAV CCTTC 8 cut(s) 94, 121, 139, 169, 253, 268, 280, 1329
HpyCH4III ACNGT 3 cut(s) 1240, 1354, 1437
HpyCH4IV ACGT 1 cut(s) 1268
HpyCH4V TGCA 7 cut(s) 506, 626, 743, 1025, 1174, 1209, 1295
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 373, 604, 1221
HpyF3I CTNAG 5 cut(s) 378, 516, 631, 1404, 1459
HpySE526I ACGT 1 cut(s) 1268
Hsp92II CATG 6 cut(s) 31, 1063, 1174, 1209, 1222, 1333
HspAI GCGC 1 cut(s) 1222
Ksp22I TGATCA 2 cut(s) 805, 1326
Kzo9I GATC 8 cut(s) 117, 130, 535, 805, 823, 865, 1288, 1326
LmnI GCTCC 5 cut(s) 55, 63, 148, 566, 1315
Lsp1109I GCAGC 5 cut(s) 472, 490, 607, 610, 1186
LweI GCATC 2 cut(s) 376, 515
MabI ACCWGGT 2 cut(s) 757, 1045
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 1268
MaeIII GTNAC 1 cut(s) 1272
MalI GATC 8 cut(s) 119, 132, 537, 807, 825, 867, 1290, 1328
MbiI CCGCTC 1 cut(s) 1484
MboI GATC 8 cut(s) 117, 130, 535, 805, 823, 865, 1288, 1326
MflI RGATCY 4 cut(s) 117, 130, 823, 865
MhlI GDGCHC 4 cut(s) 60, 190, 424, 1433
MluCI AATT 5 cut(s) 71, 471, 525, 547, 1394
MlyI GAGTC 2 cut(s) 584, 1391
MmeI TCCRAC 1 cut(s) 1285
MseI TTAA 3 cut(s) 237, 1341, 1397
MslI CAYNNNNRTG 1 cut(s) 951
MspA1I CMGCKG 2 cut(s) 344, 1463
MspI CCGG 3 cut(s) 299, 572, 1313
Mva1269I GAATGC 1 cut(s) 1408
MwoI GCNNNNNNNGC 4 cut(s) 185, 373, 604, 1221
NciI CCSGG 1 cut(s) 299
NdeI CATATG 1 cut(s) 1090
NdeII GATC 8 cut(s) 117, 130, 535, 805, 823, 865, 1288, 1326
NlaIII CATG 6 cut(s) 31, 1063, 1174, 1209, 1222, 1333
NmuCI GTSAC 1 cut(s) 1272
PagI TCATGA 1 cut(s) 27
PaqCI CACCTGC 1 cut(s) 823
PcsI WCGNNNNNNNCGW 2 cut(s) 1377, 1433
PctI GAATGC 1 cut(s) 1408
PfeI GAWTC 5 cut(s) 383, 409, 934, 1075, 1373
PflFI GACNNNGTC 1 cut(s) 1047
PflMI CCANNNNNTGG 3 cut(s) 431, 920, 1091
PfoI TCCNGGA 1 cut(s) 667
PkrI GCNGC 7 cut(s) 487, 505, 609, 622, 625, 1011, 1176
PleI GAGTC 2 cut(s) 583, 1390
PpsI GAGTC 2 cut(s) 583, 1390
Psp124BI GAGCTC 1 cut(s) 60
PspOMI GGGCCC 1 cut(s) 420
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 4 cut(s) 117, 130, 823, 865
PsyI GACNNNGTC 1 cut(s) 1047
PvuII CAGCTG 1 cut(s) 1463
RsaI GTAC 2 cut(s) 837, 1302
RsaNI GTAC 2 cut(s) 836, 1301
RseI CAYNNNNRTG 1 cut(s) 951
SacI GAGCTC 1 cut(s) 60
SalI GTCGAC 2 cut(s) 1041, 1384
SaqAI TTAA 3 cut(s) 237, 1341, 1397
SatI GCNGC 7 cut(s) 486, 504, 608, 621, 624, 1010, 1175
Sau3AI GATC 8 cut(s) 117, 130, 535, 805, 823, 865, 1288, 1326
SchI GAGTC 2 cut(s) 584, 1391
SduI GDGCHC 4 cut(s) 60, 190, 424, 1433
SexAI ACCWGGT 2 cut(s) 757, 1045
SfaNI GCATC 2 cut(s) 376, 515
SinI GGWCC 9 cut(s) 320, 464, 671, 923, 1049, 1066, 1094, 1106, 1495
SmiMI CAYNNNNRTG 1 cut(s) 951
SmlI CTYRAG 2 cut(s) 1306, 1490
SmoI CTYRAG 2 cut(s) 1306, 1490
Sse9I AATT 5 cut(s) 71, 471, 525, 547, 1394
SsiI CCGC 8 cut(s) 61, 342, 596, 608, 989, 1009, 1347, 1482
SspMI CTAG 1 cut(s) 224
SstI GAGCTC 1 cut(s) 60
StyI CCWWGG 2 cut(s) 12, 819
TaaI ACNGT 3 cut(s) 1240, 1354, 1437
TaiI ACGT 1 cut(s) 1271
TaqI TCGA 4 cut(s) 1042, 1287, 1371, 1385
TasI AATT 5 cut(s) 71, 471, 525, 547, 1394
TatI WGTACW 1 cut(s) 1300
TauI GCSGC 2 cut(s) 610, 1012
TfiI GAWTC 5 cut(s) 383, 409, 934, 1075, 1373
Tru1I TTAA 3 cut(s) 237, 1341, 1397
Tru9I TTAA 3 cut(s) 237, 1341, 1397
TscAI CASTG 4 cut(s) 520, 648, 772, 921
TseFI GTSAC 1 cut(s) 1272
TseI GCWGC 5 cut(s) 485, 503, 620, 623, 1174
Tsp45I GTSAC 1 cut(s) 1272
TspDTI ATGAA 5 cut(s) 17, 44, 263, 480, 1207
TspGWI ACGGA 2 cut(s) 53, 553
TspRI CASTG 4 cut(s) 520, 648, 772, 921
Tth111I GACNNNGTC 1 cut(s) 1047
Van91I CCANNNNNTGG 3 cut(s) 431, 920, 1091
VpaK11BI GGWCC 9 cut(s) 320, 464, 671, 923, 1049, 1066, 1094, 1106, 1495
XapI RAATTY 2 cut(s) 471, 525
XmiI GTMKAC 2 cut(s) 1042, 1385
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.