Rroxscaffold_4G00303970

Basic blue protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018473
Physical Location & Seq
Forward (+)
24479711 .. 24555778
76068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303970.1

Sequence Viewer

Length: 564 bp
ATGGCACGATACAGCACAGCGAGTCTTGGTTTAGTAATTGCCTTTTTCGTTTTTCCAAGCATGGTGTTGAGTGCACAATATTGGGTCGGAGGCGGTGTTGATGGCTGGGGAACTTGGGAAGAAGATTACATGAAGTGGGCTGCTGGCGAAACTTTCCATGTCGGAGATGTTCTCATCTTCAGCTATGATGAAAAAATACACAATCTTGTTGTAGCAGCTAATAGTGACCTATATGAGCAATTTTTTCCGATCATGGTGTTCGGAGGAACAGCTTATCACGTCGGACGTGCTGCTGACTGGAACGATCATCTGTTCGATTACCAGGGTTGGGCTTGGAGCATAAATTTCCAAGTCGGAGATTCTCTCGTTTTCAGTTACCATGCAGATGTACACTCTATCGTTGTAGCAGCTAATCGTGTACTGTATGATGGATGTTCTTCAACTCCAAACTTGGGAGTGTATCAGAGCGGATATGAAGAATTAGTCTTGCCGGTTGCTGGGACCTATTATTTCTTCTGCGGAAATCAATGCAACCAAGGAATGAAATTTGTTGTCACTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

187

Amino Acids

20.89

Weight (kDa)

4.51

Isoelectric Point (pI)

23.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 38 - 78 2.5e-09 Plastocyanin-like domain
Cu_bind_like PF02298 100 - 181 6e-17 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018473)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g12400 FvH4_7g12410
rosa_chinensis RchiOBHm_Chr1g0351221
rosa_roxburghii Rroxscaffold_4G00303960 Rroxscaffold_4G00303970
rosa_rugosa Rorug01G0217000
rosa_samantha Rh1BG201500 Rh1CG215700 Rh1DG228900
rosa_wichuraiana Rw1G020110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 468
AciI CCGC 3 cut(s) 93, 468, 519
AcsI RAATTY 2 cut(s) 343, 545
AcuI CTGAAG 1 cut(s) 163
AdeI CACNNNGTG 1 cut(s) 561
AfaI GTAC 2 cut(s) 390, 420
AfiI CCNNNNNNNGG 3 cut(s) 328, 452, 497
AgsI TTSAA 1 cut(s) 441
AjiI CACGTC 2 cut(s) 280, 287
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 4 cut(s) 183, 218, 272, 410
AluI AGCT 4 cut(s) 183, 218, 272, 410
Alw21I GWGCWC 1 cut(s) 76
Alw44I GTGCAC 1 cut(s) 72
ApaLI GTGCAC 1 cut(s) 72
ApeKI GCWGC 4 cut(s) 140, 215, 290, 407
ApoI RAATTY 2 cut(s) 343, 545
AspS9I GGNCC 1 cut(s) 501
AvaII GGWCC 1 cut(s) 501
BaeGI GKGCMC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 76
BbvI GCAGC 4 cut(s) 127, 227, 277, 419
BccI CCATC 2 cut(s) 95, 422
BciT130I CCWGG 1 cut(s) 323
BisI GCNGC 4 cut(s) 141, 216, 291, 408
BlsI GCNGC 4 cut(s) 142, 217, 292, 409
Bme1390I CCNGG 1 cut(s) 323
Bme18I GGWCC 1 cut(s) 501
BmgBI CACGTC 2 cut(s) 280, 287
BmgT120I GGNCC 1 cut(s) 501
BmiI GGNNCC 1 cut(s) 502
BmrFI CCNGG 1 cut(s) 323
BplI GAGNNNNNCTC 4 cut(s) 156, 188, 348, 380
BsaJI CCNNGG 2 cut(s) 322, 535
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 3 cut(s) 328, 452, 497
Bse118I RCCGGY 1 cut(s) 490
Bse1I ACTGG 1 cut(s) 302
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 2 cut(s) 322, 535
BseGI GGATG 1 cut(s) 437
BseLI CCNNNNNNNGG 3 cut(s) 328, 452, 497
BseNI ACTGG 1 cut(s) 302
BseSI GKGCMC 1 cut(s) 76
BseXI GCAGC 4 cut(s) 127, 227, 277, 419
BseYI CCCAGC 2 cut(s) 105, 497
BsiHKAI GWGCWC 1 cut(s) 76
BsiSI CCGG 1 cut(s) 491
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 3 cut(s) 328, 452, 497
BsmFI GGGAC 1 cut(s) 514
Bsp1286I GDGCHC 1 cut(s) 76
Bsp1407I TGTACA 1 cut(s) 388
Bsp143I GATC 2 cut(s) 249, 304
BspACI CCGC 3 cut(s) 93, 468, 519
BspLI GGNNCC 1 cut(s) 502
BsrBI CCGCTC 1 cut(s) 468
BsrFI RCCGGY 1 cut(s) 490
BsrGI TGTACA 1 cut(s) 388
BsrI ACTGG 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 2 cut(s) 322, 535
BssMI GATC 2 cut(s) 249, 304
BssT1I CCWWGG 1 cut(s) 535
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 2 cut(s) 423, 559
BstAUI TGTACA 1 cut(s) 388
BstC8I GCNNGC 1 cut(s) 145
BstF5I GGATG 1 cut(s) 437
BstKTI GATC 2 cut(s) 252, 307
BstMBI GATC 2 cut(s) 249, 304
BstNI CCWGG 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 321
BstSLI GKGCMC 1 cut(s) 76
BstV1I GCAGC 4 cut(s) 127, 227, 277, 419
BtrI CACGTC 2 cut(s) 280, 287
BtsCI GGATG 1 cut(s) 437
BtsIMutI CAGTG 1 cut(s) 555
Cac8I GCNNGC 1 cut(s) 145
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 1 cut(s) 501
Csp6I GTAC 2 cut(s) 389, 419
CviAII CATG 5 cut(s) 61, 130, 158, 253, 380
CviJI RGCY 7 cut(s) 105, 140, 183, 218, 272, 332, 410
CviKI_1 RGCY 7 cut(s) 105, 140, 183, 218, 272, 332, 410
CviQI GTAC 2 cut(s) 389, 419
DpnI GATC 2 cut(s) 251, 306
DpnII GATC 2 cut(s) 249, 304
DraIII CACNNNGTG 1 cut(s) 561
Eco130I CCWWGG 1 cut(s) 535
Eco47I GGWCC 1 cut(s) 501
Eco57I CTGAAG 1 cut(s) 163
EcoO109I RGGNCCY 1 cut(s) 501
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 535
ErhI CCWWGG 1 cut(s) 535
FaeI CATG 5 cut(s) 64, 133, 161, 256, 383
FaqI GGGAC 1 cut(s) 514
FatI CATG 5 cut(s) 60, 129, 157, 252, 379
Fnu4HI GCNGC 4 cut(s) 141, 216, 291, 408
FokI GGATG 1 cut(s) 444
Fsp4HI GCNGC 4 cut(s) 141, 216, 291, 408
GluI GCNGC 4 cut(s) 141, 216, 291, 408
GsaI CCCAGC 2 cut(s) 109, 501
HapII CCGG 1 cut(s) 491
Hin1II CATG 5 cut(s) 64, 133, 161, 256, 383
HinfI GANTC 2 cut(s) 22, 359
HpaII CCGG 1 cut(s) 491
Hpy166II GTNNAC 3 cut(s) 74, 391, 419
Hpy188I TCNGA 7 cut(s) 89, 164, 249, 263, 284, 356, 465
Hpy8I GTNNAC 3 cut(s) 74, 391, 419
Hpy99I CGWCG 1 cut(s) 284
HpyCH4III ACNGT 2 cut(s) 423, 559
HpyCH4IV ACGT 2 cut(s) 279, 286
HpyCH4V TGCA 3 cut(s) 74, 383, 531
HpySE526I ACGT 2 cut(s) 279, 286
Hsp92II CATG 5 cut(s) 64, 133, 161, 256, 383
Kzo9I GATC 2 cut(s) 249, 304
LmnI GCTCC 1 cut(s) 336
LpnPI CCDG 7 cut(s) 91, 129, 283, 308, 335, 483, 504
Lsp1109I GCAGC 4 cut(s) 127, 227, 277, 419
MaeII ACGT 2 cut(s) 279, 286
MaeIII GTNAC 3 cut(s) 224, 374, 553
MalI GATC 2 cut(s) 251, 306
MbiI CCGCTC 1 cut(s) 468
MboI GATC 2 cut(s) 249, 304
MboII GAAGA 6 cut(s) 131, 134, 169, 429, 488, 505
MhlI GDGCHC 1 cut(s) 76
MluCI AATT 5 cut(s) 36, 239, 343, 479, 545
MlyI GAGTC 1 cut(s) 31
MmeI TCCRAC 4 cut(s) 67, 142, 262, 334
MnlI CCTC 2 cut(s) 83, 257
MslI CAYNNNNRTG 1 cut(s) 384
MspI CCGG 1 cut(s) 491
MspR9I CCNGG 1 cut(s) 323
MvaI CCWGG 1 cut(s) 323
NdeII GATC 2 cut(s) 249, 304
NlaIII CATG 5 cut(s) 64, 133, 161, 256, 383
NlaIV GGNNCC 1 cut(s) 502
NmuCI GTSAC 2 cut(s) 224, 553
PfeI GAWTC 1 cut(s) 359
PkrI GCNGC 4 cut(s) 142, 217, 292, 409
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
PpuMI RGGWCCY 1 cut(s) 501
Psp5II RGGWCCY 1 cut(s) 501
Psp6I CCWGG 1 cut(s) 321
PspFI CCCAGC 2 cut(s) 105, 497
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 1 cut(s) 502
PspPI GGNCC 1 cut(s) 501
PspPPI RGGWCCY 1 cut(s) 501
RsaI GTAC 2 cut(s) 390, 420
RsaNI GTAC 2 cut(s) 389, 419
RseI CAYNNNNRTG 1 cut(s) 384
SatI GCNGC 4 cut(s) 141, 216, 291, 408
Sau3AI GATC 2 cut(s) 249, 304
Sau96I GGNCC 1 cut(s) 501
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 1 cut(s) 76
SetI ASST 8 cut(s) 185, 220, 231, 274, 282, 289, 412, 506
SinI GGWCC 1 cut(s) 501
SmiMI CAYNNNNRTG 1 cut(s) 384
Sse9I AATT 5 cut(s) 36, 239, 343, 479, 545
SsiI CCGC 3 cut(s) 93, 468, 519
SspI AATATT 1 cut(s) 80
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 2 cut(s) 423, 559
TaiI ACGT 2 cut(s) 282, 289
TaqI TCGA 1 cut(s) 315
TasI AATT 5 cut(s) 36, 239, 343, 479, 545
TatI WGTACW 2 cut(s) 388, 418
TfiI GAWTC 1 cut(s) 359
TscAI CASTG 1 cut(s) 562
TseFI GTSAC 2 cut(s) 224, 553
TseI GCWGC 4 cut(s) 140, 215, 290, 407
Tsp45I GTSAC 2 cut(s) 224, 553
TspDTI ATGAA 4 cut(s) 146, 204, 489, 557
TspRI CASTG 1 cut(s) 562
VneI GTGCAC 1 cut(s) 72
VpaK11BI GGWCC 1 cut(s) 501
XapI RAATTY 2 cut(s) 343, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.