Rroxscaffold_4G00315190

phytol kinase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012066
Physical Location & Seq
Reverse (-)
39404242 .. 39405837
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00315190.1

Sequence Viewer

Length: 930 bp
ATGACCCTCTTATCCTTCACCCCTTCTCGCCACCTCAACCCAACCACCACTTTCGTCTCTATCGTACGCCGTCCCCCTCTCAACCCAGTTCCCTCCTCCCTCTTCTTCTGCCCCACCTCCCCTCCACTTCCGCTCCACATCCTCTACCGCCCCCCACGCCCCTCCCTTTCCCTCGTCTCCGCTCCACGCGCCGCCTCCTCCGCCGGGCACCTCTTGCATGATGCCGGAGCCACCGCCCTCGTACTCGCCGGCGCCTACACTCTCGTCCTCTGCTTCGATAATCTCACCAAGCGAAACCTCCTCCAACAGTTTGAGCAGAAAATTGGTCCACATATCATCTGGGCTGCTCTTCGTGCTTTCCTGGCCAATTTTCAGAATAGCACGTCAACAGAAGCTCGCTACTTTGCCTCTGTGGTTCCCCTCTTTAATGGCTTGAGGCTTCTTCTGGGTGGCTTATCCTTGGTCCCCAATGAAGGACTAGTCAAATCCGTCACTCGTGAAGGAAACCCAAAGGAGCTGCTTAGGGGACCCCTGTATTATGTTCTTATACTGATCTTATCTGCTCTAGTGTTTTGGCGCGAGTCTCCGGTTGGAGTGATCTCCTTAGCAATGATGTGTGGTGGTGATGGTGTTGCTGATATCATGGGTAGAAGATTTGGGTCCATAAAAATCCCCTATAATCAAAAGAAGAGTTGGGCTGGGAGCATTTCCATGTTTGTTTTTGGGTCCTTGGTTTCCCTTGGGATGCTCTACTATTACTCATGTTTGGGATATTTCAATTTGGATTGGATGGATACGGTGCAAAAGGTTGCTTTCGTTTCTTTGGTGGCTACAGCGGTAGAATCCCTTCCGATTACAGAGGTAGTGGATGACAATATATCTGTTCCATTAGCAAGTATGGCAGCTGCATATTTGAGTTTTTCTTTGTAG

Protein Analysis

309

Amino Acids

33.67

Weight (kDa)

9.32

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04490 AT5G04490
fragaria_vesca FvH4_7g06990 FvH4_7g06990 FvH4_7g06990
malus_domestica MD07G1076000.v1.1
prunus_persica Prupe.2G096700_v2.0.a1 Prupe.2G096700_v2.0.a1
pyrus_communis pycom07g05990
rosa_chinensis RchiOBHm_Chr1g0336921
rosa_laevigata RLG00000029322
rosa_roxburghii Rroxscaffold_4G00315190
rosa_rugosa Rorug01G0128900 Rorug01G0128900
rosa_samantha Rh1AG149900 Rh1CG141100 Rh1DG157000
rosa_wichuraiana Rw1G012110 Rw1G012220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 207, 251
AccBSI CCGCTC 2 cut(s) 133, 182
AccII CGCG 2 cut(s) 189, 579
AciI CCGC 7 cut(s) 131, 148, 180, 192, 201, 234, 836
AcoI YGGCCR 1 cut(s) 363
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 2 cut(s) 66, 243
AfiI CCNNNNNNNGG 2 cut(s) 204, 473
AgsI TTSAA 1 cut(s) 778
AhlI ACTAGT 1 cut(s) 478
AjiI CACGTC 1 cut(s) 384
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 3 cut(s) 395, 517, 905
AluI AGCT 3 cut(s) 395, 517, 905
Alw26I GTCTC 3 cut(s) 61, 181, 588
AoxI GGCC 1 cut(s) 363
ApeKI GCWGC 4 cut(s) 344, 517, 902, 905
Asp700I GAANNNNTTC 1 cut(s) 846
AspLEI GCGC 3 cut(s) 191, 254, 579
AspS9I GGNCC 5 cut(s) 326, 463, 527, 660, 726
AsuC2I CCSGG 1 cut(s) 205
AsuHPI GGTGA 3 cut(s) 10, 277, 635
AvaII GGWCC 5 cut(s) 326, 463, 527, 660, 726
BaeGI GKGCMC 1 cut(s) 210
BalI TGGCCA 1 cut(s) 365
BanI GGYRCC 2 cut(s) 207, 251
BarI GAAGNNNNNNTAC 2 cut(s) 831, 863
BauI CACGAG 1 cut(s) 495
BbvI GCAGC 4 cut(s) 331, 504, 892, 914
BccI CCATC 2 cut(s) 620, 784
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 1 cut(s) 362
BciVI GTATCC 1 cut(s) 787
BcnI CCSGG 1 cut(s) 205
BcoDI GTCTC 3 cut(s) 61, 181, 588
BcuI ACTAGT 1 cut(s) 478
BfaI CTAG 2 cut(s) 479, 566
BfmI CTRYAG 1 cut(s) 831
BfoI RGCGCY 1 cut(s) 255
BfuI GTATCC 1 cut(s) 787
BisI GCNGC 5 cut(s) 192, 345, 518, 903, 906
BlsI GCNGC 5 cut(s) 193, 346, 519, 904, 907
Bme1390I CCNGG 2 cut(s) 205, 362
Bme18I GGWCC 5 cut(s) 326, 463, 527, 660, 726
BmgBI CACGTC 1 cut(s) 384
BmgT120I GGNCC 5 cut(s) 326, 463, 527, 660, 726
BmiI GGNNCC 9 cut(s) 209, 229, 253, 417, 465, 528, 529, 661, 727
BmrFI CCNGG 2 cut(s) 205, 362
BmrI ACTGGG 1 cut(s) 80
BmsI GCATC 2 cut(s) 211, 735
BmuI ACTGGG 1 cut(s) 80
Bpu10I CCTNAGC 2 cut(s) 521, 604
BpuEI CTTGAG 1 cut(s) 454
BpuMI CCSGG 1 cut(s) 205
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 3 cut(s) 459, 729, 739
BsaWI WCCGGW 1 cut(s) 586
BsaXI ACNNNNNCTCC 4 cut(s) 106, 117, 136, 147
Bsc4I CCNNNNNNNGG 2 cut(s) 204, 473
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 86
Bse3DI GCAATG 1 cut(s) 615
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 3 cut(s) 459, 729, 739
BseGI GGATG 4 cut(s) 138, 750, 795, 874
BseLI CCNNNNNNNGG 2 cut(s) 204, 473
BseMI GCAATG 1 cut(s) 615
BseNI ACTGG 1 cut(s) 86
BseRI GAGGAG 3 cut(s) 85, 187, 290
BseSI GKGCMC 1 cut(s) 210
BseXI GCAGC 4 cut(s) 331, 504, 892, 914
BseYI CCCAGC 1 cut(s) 698
Bsh1236I CGCG 2 cut(s) 189, 579
BshFI GGCC 1 cut(s) 365
BshNI GGYRCC 2 cut(s) 207, 251
BsiSI CCGG 4 cut(s) 204, 225, 249, 587
BsiWI CGTACG 1 cut(s) 64
BslFI GGGAC 3 cut(s) 57, 449, 540
BslI CCNNNNNNNGG 2 cut(s) 204, 473
BsmAI GTCTC 3 cut(s) 61, 181, 588
BsmBI CGTCTC 2 cut(s) 61, 181
BsmFI GGGAC 3 cut(s) 57, 449, 540
BsnI GGCC 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 2 cut(s) 552, 597
BspACI CCGC 7 cut(s) 131, 148, 180, 192, 201, 234, 836
BspANI GGCC 1 cut(s) 365
BspFNI CGCG 2 cut(s) 189, 579
BspLI GGNNCC 9 cut(s) 209, 229, 253, 417, 465, 528, 529, 661, 727
BspQI GCTCTTC 1 cut(s) 354
BspT107I GGYRCC 2 cut(s) 207, 251
BsrBI CCGCTC 2 cut(s) 133, 182
BsrDI GCAATG 1 cut(s) 615
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 459, 729, 739
BssMI GATC 2 cut(s) 552, 597
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 495
BssT1I CCWWGG 3 cut(s) 459, 729, 739
Bst2BI CACGAG 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 309, 799
Bst6I CTCTTC 3 cut(s) 107, 354, 683
BstACI GRCGYC 1 cut(s) 252
BstAPI GCANNNNNTGC 1 cut(s) 214
BstC8I GCNNGC 2 cut(s) 250, 397
BstDEI CTNAG 2 cut(s) 521, 604
BstF5I GGATG 4 cut(s) 138, 750, 795, 874
BstFNI CGCG 2 cut(s) 189, 579
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 3 cut(s) 191, 254, 579
BstKTI GATC 2 cut(s) 555, 600
BstMAI GTCTC 3 cut(s) 61, 181, 588
BstMBI GATC 2 cut(s) 552, 597
BstMWI GCNNNNNNNGC 7 cut(s) 156, 188, 200, 214, 353, 362, 899
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 2 cut(s) 203, 360
BstSFI CTRYAG 1 cut(s) 831
BstSLI GKGCMC 1 cut(s) 210
BstUI CGCG 2 cut(s) 189, 579
BstV1I GCAGC 4 cut(s) 331, 504, 892, 914
BsuI GTATCC 1 cut(s) 787
BsuRI GGCC 1 cut(s) 365
BtrI CACGTC 1 cut(s) 384
BtsCI GGATG 4 cut(s) 138, 750, 795, 874
Cac8I GCNNGC 2 cut(s) 250, 397
CfoI GCGC 3 cut(s) 191, 254, 579
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 5 cut(s) 326, 463, 527, 660, 726
Csp6I GTAC 2 cut(s) 65, 242
CviAII CATG 4 cut(s) 218, 643, 712, 762
CviQI GTAC 2 cut(s) 65, 242
DdeI CTNAG 2 cut(s) 521, 604
DinI GGCGCC 1 cut(s) 253
DpnI GATC 2 cut(s) 554, 599
DpnII GATC 2 cut(s) 552, 597
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 3 cut(s) 107, 354, 683
EarI CTCTTC 3 cut(s) 107, 354, 683
EciI GGCGGA 1 cut(s) 190
Eco130I CCWWGG 3 cut(s) 459, 729, 739
Eco32I GATATC 1 cut(s) 640
Eco47I GGWCC 5 cut(s) 326, 463, 527, 660, 726
EcoO109I RGGNCCY 2 cut(s) 527, 726
EcoRII CCWGG 1 cut(s) 360
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 3 cut(s) 459, 729, 739
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 3 cut(s) 459, 729, 739
Esp3I CGTCTC 2 cut(s) 61, 181
FaeI CATG 4 cut(s) 221, 646, 715, 765
FaqI GGGAC 3 cut(s) 57, 449, 540
FatI CATG 4 cut(s) 217, 642, 711, 761
Fnu4HI GCNGC 5 cut(s) 192, 345, 518, 903, 906
FokI GGATG 4 cut(s) 125, 757, 802, 881
Fsp4HI GCNGC 5 cut(s) 192, 345, 518, 903, 906
FspBI CTAG 2 cut(s) 479, 566
GlaI GCGC 3 cut(s) 190, 253, 578
GluI GCNGC 5 cut(s) 192, 345, 518, 903, 906
GsaI CCCAGC 1 cut(s) 702
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 1 cut(s) 365
HapII CCGG 4 cut(s) 204, 225, 249, 587
HhaI GCGC 3 cut(s) 191, 254, 579
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 4 cut(s) 221, 646, 715, 765
Hin6I GCGC 3 cut(s) 189, 252, 577
HinP1I GCGC 3 cut(s) 189, 252, 577
HincII GTYRAC 1 cut(s) 387
HindII GTYRAC 1 cut(s) 387
HinfI GANTC 2 cut(s) 581, 842
HpaII CCGG 4 cut(s) 204, 225, 249, 587
HphI GGTGA 3 cut(s) 10, 277, 635
Hpy166II GTNNAC 2 cut(s) 329, 387
Hpy188I TCNGA 2 cut(s) 375, 852
Hpy188III TCNNGA 1 cut(s) 497
Hpy8I GTNNAC 2 cut(s) 329, 387
HpyAV CCTTC 5 cut(s) 25, 33, 467, 494, 857
HpyCH4III ACNGT 2 cut(s) 309, 799
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 3 cut(s) 217, 802, 908
HpyF10VI GCNNNNNNNGC 7 cut(s) 156, 188, 200, 214, 353, 362, 899
HpyF3I CTNAG 2 cut(s) 521, 604
HpySE526I ACGT 1 cut(s) 383
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 4 cut(s) 221, 646, 715, 765
HspAI GCGC 3 cut(s) 189, 252, 577
KasI GGCGCC 1 cut(s) 251
KflI GGGWCCC 1 cut(s) 527
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Kzo9I GATC 2 cut(s) 552, 597
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 5 cut(s) 138, 187, 227, 514, 702
Lsp1109I GCAGC 4 cut(s) 331, 504, 892, 914
LweI GCATC 2 cut(s) 211, 735
MaeI CTAG 2 cut(s) 479, 566
MaeII ACGT 1 cut(s) 383
MaeIII GTNAC 1 cut(s) 490
MalI GATC 2 cut(s) 554, 599
MbiI CCGCTC 2 cut(s) 133, 182
MboI GATC 2 cut(s) 552, 597
MboII GAAGA 6 cut(s) 94, 97, 341, 434, 663, 700
MhlI GDGCHC 1 cut(s) 210
MlsI TGGCCA 1 cut(s) 365
MluCI AATT 3 cut(s) 321, 367, 778
MluNI TGGCCA 1 cut(s) 365
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 1 cut(s) 590
MmeI TCCRAC 2 cut(s) 328, 571
Mox20I TGGCCA 1 cut(s) 365
MreI CGCCGGCG 1 cut(s) 248
MroNI GCCGGC 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 846
MscI TGGCCA 1 cut(s) 365
MseI TTAA 1 cut(s) 426
MslI CAYNNNNRTG 1 cut(s) 710
Msp20I TGGCCA 1 cut(s) 365
MspA1I CMGCKG 2 cut(s) 836, 905
MspI CCGG 4 cut(s) 204, 225, 249, 587
MspR9I CCNGG 2 cut(s) 205, 362
MvaI CCWGG 1 cut(s) 362
MvnI CGCG 2 cut(s) 189, 579
MwoI GCNNNNNNNGC 7 cut(s) 156, 188, 200, 214, 353, 362, 899
NaeI GCCGGC 1 cut(s) 250
NarI GGCGCC 1 cut(s) 252
NciI CCSGG 1 cut(s) 205
NdeII GATC 2 cut(s) 552, 597
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 4 cut(s) 221, 646, 715, 765
NlaIV GGNNCC 9 cut(s) 209, 229, 253, 417, 465, 528, 529, 661, 727
NmuCI GTSAC 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 354
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PdiI GCCGGC 1 cut(s) 250
PdmI GAANNNNTTC 1 cut(s) 846
PfeI GAWTC 1 cut(s) 842
Pfl23II CGTACG 1 cut(s) 64
PkrI GCNGC 5 cut(s) 193, 346, 519, 904, 907
PleI GAGTC 1 cut(s) 589
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 589
PpuMI RGGWCCY 2 cut(s) 527, 726
Psp5II RGGWCCY 2 cut(s) 527, 726
Psp6I CCWGG 1 cut(s) 360
PspFI CCCAGC 1 cut(s) 698
PspGI CCWGG 1 cut(s) 360
PspLI CGTACG 1 cut(s) 64
PspN4I GGNNCC 9 cut(s) 209, 229, 253, 417, 465, 528, 529, 661, 727
PspPI GGNCC 5 cut(s) 326, 463, 527, 660, 726
PspPPI RGGWCCY 2 cut(s) 527, 726
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 2 cut(s) 66, 243
RsaNI GTAC 2 cut(s) 65, 242
RseI CAYNNNNRTG 1 cut(s) 710
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 5 cut(s) 192, 345, 518, 903, 906
Sau3AI GATC 2 cut(s) 552, 597
Sau96I GGNCC 5 cut(s) 326, 463, 527, 660, 726
SchI GAGTC 1 cut(s) 590
ScrFI CCNGG 2 cut(s) 205, 362
SduI GDGCHC 1 cut(s) 210
SfaNI GCATC 2 cut(s) 211, 735
SfcI CTRYAG 1 cut(s) 831
SfoI GGCGCC 1 cut(s) 253
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 5 cut(s) 326, 463, 527, 660, 726
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 1 cut(s) 433
SmoI CTYRAG 1 cut(s) 433
SpeI ACTAGT 1 cut(s) 478
Sse9I AATT 3 cut(s) 321, 367, 778
SsiI CCGC 7 cut(s) 131, 148, 180, 192, 201, 234, 836
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 2 cut(s) 479, 566
StyD4I CCNGG 2 cut(s) 203, 360
StyI CCWWGG 3 cut(s) 459, 729, 739
TaaI ACNGT 2 cut(s) 309, 799
TaiI ACGT 1 cut(s) 386
TaqI TCGA 1 cut(s) 276
TasI AATT 3 cut(s) 321, 367, 778
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 1 cut(s) 842
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TseFI GTSAC 1 cut(s) 490
TseI GCWGC 4 cut(s) 344, 517, 902, 905
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 1 cut(s) 486
TspGWI ACGGA 1 cut(s) 478
VpaK11BI GGWCC 5 cut(s) 326, 463, 527, 660, 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 336
XmnI GAANNNNTTC 1 cut(s) 846
XspI CTAG 2 cut(s) 479, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.