Rw1G012220

phytol kinase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0012066
Physical Location & Seq
Forward (+)
27342162 .. 27343515
1354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G012220.1

Sequence Viewer

Length: 945 bp
ATGACCCTCTTATCCTTCACCCCCTCTTGCCACCTTAACCTCAACCTCAACCCCAACCCCACCACCACTTTCTTCTCTATCGTACGCCGTCCCCCTCTCAACCCAGTTCCCCCCTCCCTCTTCTTCTGCCCCACCTCCCCTCCACTTCCGCTCCACATCCTCTACCGCCCCCCACGCCCCCCCCTTTCCCTCGTCTCCGCTCCACGCGCCGCCTCCTCCGCCGGGCACCTCTTGCATGATGCCGGAGCCACCGCCCTCGTACTCGCCGGCGCCTACACTCTCGTCCTCTGCTTCGATAATCTCACCAAGCGAAACCTCCTCCAACAGAGTTTGAGCAGAAAATTGGTCCACATATCATCTGGGCTGCTCTTCGTGCTTTCCTGGCCAATTTTCAGCACGTCAACAGAAGCTCGCTACTTTGCCTCTGTGGTTCCCCTCTTTAATGGCTTGAGGCTTCTTCTTAGTGGCCTCTCCTTGGTCCCCAATGAAGGACTAGTCAAATCCGTCACTCGTGAAGGAAACCCAAAGGAGTTGCTTAGGGGACCCTTGTATTATGTTCTTATACTGATCTTATCTGCTCTAGTGTTTTGGCGCGAGTCTCCGGTTGGAGTGATCTCCTTAGCAATGATGTGTGGTGGTGATGGTGTTGCTGATATCATGGGTAGAAGATTCGGGTCCATAAAGATCCCCTATAATCAAAAGAAGAGTTGGGCTGGGAGCATTTCCATGTTTGTTTTTGGGTTCTTGGTTTCCCTTGGGATGCTTTACTATTACTCAATTTTGGGATATTTCAATTTGGATTGGATGGATACGGTGCAAAAGGTTGCTTTCGTTTCTTTGGTGGCTACAGTAGTAGAATCCCTTCCTATTACAAAGGTAGTGGATGACAATATATCTGTTCCATTGGCAAGTATGGCAGCCGCATATTTCAGTTTTTCTTTGTAG

Protein Analysis

314

Amino Acids

34.33

Weight (kDa)

9.51

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04490 AT5G04490
fragaria_vesca FvH4_7g06990 FvH4_7g06990 FvH4_7g06990
malus_domestica MD07G1076000.v1.1
prunus_persica Prupe.2G096700_v2.0.a1 Prupe.2G096700_v2.0.a1
pyrus_communis pycom07g05990
rosa_chinensis RchiOBHm_Chr1g0336921
rosa_laevigata RLG00000029322
rosa_roxburghii Rroxscaffold_4G00315190
rosa_rugosa Rorug01G0128900 Rorug01G0128900
rosa_samantha Rh1AG149900 Rh1CG141100 Rh1DG157000
rosa_wichuraiana Rw1G012110 Rw1G012220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 225, 269
AccBSI CCGCTC 2 cut(s) 151, 200
AccII CGCG 2 cut(s) 207, 594
AciI CCGC 7 cut(s) 149, 166, 198, 210, 219, 252, 921
AclWI GGATC 1 cut(s) 679
AcoI YGGCCR 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 2 cut(s) 84, 261
AfiI CCNNNNNNNGG 3 cut(s) 222, 475, 488
AgsI TTSAA 1 cut(s) 793
AhlI ACTAGT 1 cut(s) 493
AjiI CACGTC 1 cut(s) 399
AjnI CCWGG 1 cut(s) 380
AluBI AGCT 1 cut(s) 410
AluI AGCT 1 cut(s) 410
Alw26I GTCTC 2 cut(s) 199, 603
AlwI GGATC 1 cut(s) 679
AoxI GGCC 2 cut(s) 383, 466
ApeKI GCWGC 2 cut(s) 364, 917
Asp700I GAANNNNTTC 1 cut(s) 861
AspLEI GCGC 3 cut(s) 209, 272, 594
AspS9I GGNCC 4 cut(s) 346, 478, 542, 675
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 3 cut(s) 10, 295, 650
AvaII GGWCC 4 cut(s) 346, 478, 542, 675
BaeGI GKGCMC 1 cut(s) 228
BalI TGGCCA 1 cut(s) 385
BanI GGYRCC 2 cut(s) 225, 269
BarI GAAGNNNNNNTAC 2 cut(s) 846, 878
BauI CACGAG 1 cut(s) 510
BbvI GCAGC 2 cut(s) 351, 929
BccI CCATC 2 cut(s) 635, 799
BceAI ACGGC 1 cut(s) 72
BciT130I CCWGG 1 cut(s) 382
BciVI GTATCC 1 cut(s) 802
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 2 cut(s) 199, 603
BcuI ACTAGT 1 cut(s) 493
BfaI CTAG 2 cut(s) 494, 581
BfmI CTRYAG 1 cut(s) 846
BfoI RGCGCY 1 cut(s) 273
BfuI GTATCC 1 cut(s) 802
BisI GCNGC 4 cut(s) 210, 365, 918, 921
BlsI GCNGC 4 cut(s) 211, 366, 919, 922
Bme1390I CCNGG 2 cut(s) 223, 382
Bme18I GGWCC 4 cut(s) 346, 478, 542, 675
BmgBI CACGTC 1 cut(s) 399
BmgT120I GGNCC 4 cut(s) 346, 478, 542, 675
BmiI GGNNCC 8 cut(s) 227, 247, 271, 432, 480, 543, 544, 676
BmrFI CCNGG 2 cut(s) 223, 382
BmrI ACTGGG 1 cut(s) 98
BmsI GCATC 2 cut(s) 229, 750
BmuI ACTGGG 1 cut(s) 98
Bpu10I CCTNAGC 2 cut(s) 536, 619
BpuEI CTTGAG 1 cut(s) 469
BpuMI CCSGG 1 cut(s) 223
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 2 cut(s) 474, 754
BsaWI WCCGGW 1 cut(s) 601
BsaXI ACNNNNNCTCC 4 cut(s) 124, 135, 154, 165
Bsc4I CCNNNNNNNGG 3 cut(s) 222, 475, 488
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 104
Bse3DI GCAATG 1 cut(s) 630
BseBI CCWGG 1 cut(s) 382
BseDI CCNNGG 2 cut(s) 474, 754
BseGI GGATG 4 cut(s) 156, 765, 810, 889
BseLI CCNNNNNNNGG 3 cut(s) 222, 475, 488
BseMI GCAATG 1 cut(s) 630
BseNI ACTGG 1 cut(s) 104
BseRI GAGGAG 2 cut(s) 205, 308
BseSI GKGCMC 1 cut(s) 228
BseXI GCAGC 2 cut(s) 351, 929
BseYI CCCAGC 1 cut(s) 713
Bsh1236I CGCG 2 cut(s) 207, 594
BshFI GGCC 2 cut(s) 385, 468
BshNI GGYRCC 2 cut(s) 225, 269
BsiSI CCGG 4 cut(s) 222, 243, 267, 602
BsiWI CGTACG 1 cut(s) 82
BslFI GGGAC 3 cut(s) 75, 464, 555
BslI CCNNNNNNNGG 3 cut(s) 222, 475, 488
BsmAI GTCTC 2 cut(s) 199, 603
BsmBI CGTCTC 1 cut(s) 199
BsmFI GGGAC 3 cut(s) 75, 464, 555
BsnI GGCC 2 cut(s) 385, 468
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 3 cut(s) 567, 612, 684
BspACI CCGC 7 cut(s) 149, 166, 198, 210, 219, 252, 921
BspANI GGCC 2 cut(s) 385, 468
BspFNI CGCG 2 cut(s) 207, 594
BspLI GGNNCC 8 cut(s) 227, 247, 271, 432, 480, 543, 544, 676
BspPI GGATC 1 cut(s) 679
BspQI GCTCTTC 1 cut(s) 374
BspT107I GGYRCC 2 cut(s) 225, 269
BsrBI CCGCTC 2 cut(s) 151, 200
BsrDI GCAATG 1 cut(s) 630
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 1 cut(s) 104
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 2 cut(s) 474, 754
BssMI GATC 3 cut(s) 567, 612, 684
BssNI GRCGYC 1 cut(s) 270
BssSI CACGAG 1 cut(s) 510
BssT1I CCWWGG 2 cut(s) 474, 754
Bst2BI CACGAG 1 cut(s) 510
Bst2UI CCWGG 1 cut(s) 382
Bst4CI ACNGT 2 cut(s) 814, 850
Bst6I CTCTTC 3 cut(s) 125, 374, 698
BstACI GRCGYC 1 cut(s) 270
BstAPI GCANNNNNTGC 1 cut(s) 232
BstC8I GCNNGC 2 cut(s) 268, 412
BstDEI CTNAG 3 cut(s) 461, 536, 619
BstF5I GGATG 4 cut(s) 156, 765, 810, 889
BstFNI CGCG 2 cut(s) 207, 594
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 3 cut(s) 209, 272, 594
BstKTI GATC 3 cut(s) 570, 615, 687
BstMAI GTCTC 2 cut(s) 199, 603
BstMBI GATC 3 cut(s) 567, 612, 684
BstMWI GCNNNNNNNGC 7 cut(s) 174, 206, 218, 232, 373, 382, 914
BstNI CCWGG 1 cut(s) 382
BstSCI CCNGG 2 cut(s) 221, 380
BstSFI CTRYAG 1 cut(s) 846
BstSLI GKGCMC 1 cut(s) 228
BstUI CGCG 2 cut(s) 207, 594
BstV1I GCAGC 2 cut(s) 351, 929
BstX2I RGATCY 1 cut(s) 684
BstYI RGATCY 1 cut(s) 684
BsuI GTATCC 1 cut(s) 802
BsuRI GGCC 2 cut(s) 385, 468
BtrI CACGTC 1 cut(s) 399
BtsCI GGATG 4 cut(s) 156, 765, 810, 889
Cac8I GCNNGC 2 cut(s) 268, 412
CfoI GCGC 3 cut(s) 209, 272, 594
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 4 cut(s) 346, 478, 542, 675
Csp6I GTAC 2 cut(s) 83, 260
CspCI CAANNNNNGTGG 2 cut(s) 861, 896
CviAII CATG 3 cut(s) 236, 658, 727
CviQI GTAC 2 cut(s) 83, 260
DdeI CTNAG 3 cut(s) 461, 536, 619
DinI GGCGCC 1 cut(s) 271
DpnI GATC 3 cut(s) 569, 614, 686
DpnII GATC 3 cut(s) 567, 612, 684
EaeI YGGCCR 1 cut(s) 383
Eam1104I CTCTTC 3 cut(s) 125, 374, 698
EarI CTCTTC 3 cut(s) 125, 374, 698
EciI GGCGGA 1 cut(s) 208
Eco130I CCWWGG 2 cut(s) 474, 754
Eco32I GATATC 1 cut(s) 655
Eco47I GGWCC 4 cut(s) 346, 478, 542, 675
EcoO109I RGGNCCY 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 380
EcoRV GATATC 1 cut(s) 655
EcoT14I CCWWGG 2 cut(s) 474, 754
EgeI GGCGCC 1 cut(s) 271
EheI GGCGCC 1 cut(s) 271
ErhI CCWWGG 2 cut(s) 474, 754
Esp3I CGTCTC 1 cut(s) 199
FaeI CATG 3 cut(s) 239, 661, 730
FaqI GGGAC 3 cut(s) 75, 464, 555
FatI CATG 3 cut(s) 235, 657, 726
Fnu4HI GCNGC 4 cut(s) 210, 365, 918, 921
FokI GGATG 4 cut(s) 143, 772, 817, 896
Fsp4HI GCNGC 4 cut(s) 210, 365, 918, 921
FspBI CTAG 2 cut(s) 494, 581
GlaI GCGC 3 cut(s) 208, 271, 593
GluI GCNGC 4 cut(s) 210, 365, 918, 921
GsaI CCCAGC 1 cut(s) 717
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 2 cut(s) 385, 468
HapII CCGG 4 cut(s) 222, 243, 267, 602
HhaI GCGC 3 cut(s) 209, 272, 594
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 3 cut(s) 239, 661, 730
Hin6I GCGC 3 cut(s) 207, 270, 592
HinP1I GCGC 3 cut(s) 207, 270, 592
HincII GTYRAC 1 cut(s) 402
HindII GTYRAC 1 cut(s) 402
HinfI GANTC 3 cut(s) 596, 669, 857
HpaII CCGG 4 cut(s) 222, 243, 267, 602
HphI GGTGA 3 cut(s) 10, 295, 650
Hpy166II GTNNAC 2 cut(s) 349, 402
Hpy188III TCNNGA 1 cut(s) 512
Hpy8I GTNNAC 2 cut(s) 349, 402
HpyAV CCTTC 4 cut(s) 25, 482, 509, 872
HpyCH4III ACNGT 2 cut(s) 814, 850
HpyCH4IV ACGT 1 cut(s) 398
HpyCH4V TGCA 2 cut(s) 235, 817
HpyF10VI GCNNNNNNNGC 7 cut(s) 174, 206, 218, 232, 373, 382, 914
HpyF3I CTNAG 3 cut(s) 461, 536, 619
HpySE526I ACGT 1 cut(s) 398
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 3 cut(s) 239, 661, 730
HspAI GCGC 3 cut(s) 207, 270, 592
KasI GGCGCC 1 cut(s) 269
KflI GGGWCCC 1 cut(s) 542
KroI GCCGGC 1 cut(s) 266
KroNI GCCGGC 1 cut(s) 268
Kzo9I GATC 3 cut(s) 567, 612, 684
LguI GCTCTTC 1 cut(s) 374
LmnI GCTCC 4 cut(s) 156, 205, 245, 717
LpnPI CCDG 9 cut(s) 117, 235, 256, 280, 345, 367, 394, 615, 699
Lsp1109I GCAGC 2 cut(s) 351, 929
LweI GCATC 2 cut(s) 229, 750
MaeI CTAG 2 cut(s) 494, 581
MaeII ACGT 1 cut(s) 398
MaeIII GTNAC 1 cut(s) 505
MalI GATC 3 cut(s) 569, 614, 686
MbiI CCGCTC 2 cut(s) 151, 200
MboI GATC 3 cut(s) 567, 612, 684
MboII GAAGA 7 cut(s) 64, 112, 115, 361, 449, 678, 715
MflI RGATCY 1 cut(s) 684
MhlI GDGCHC 1 cut(s) 228
MlsI TGGCCA 1 cut(s) 385
MluCI AATT 4 cut(s) 341, 387, 777, 793
MluNI TGGCCA 1 cut(s) 385
Mly113I GGCGCC 1 cut(s) 270
MlyI GAGTC 1 cut(s) 605
MmeI TCCRAC 2 cut(s) 346, 586
Mox20I TGGCCA 1 cut(s) 385
MreI CGCCGGCG 1 cut(s) 266
MroNI GCCGGC 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 861
MscI TGGCCA 1 cut(s) 385
MseI TTAA 2 cut(s) 36, 441
MslI CAYNNNNRTG 1 cut(s) 725
Msp20I TGGCCA 1 cut(s) 385
MspI CCGG 4 cut(s) 222, 243, 267, 602
MspR9I CCNGG 2 cut(s) 223, 382
MvaI CCWGG 1 cut(s) 382
MvnI CGCG 2 cut(s) 207, 594
MwoI GCNNNNNNNGC 7 cut(s) 174, 206, 218, 232, 373, 382, 914
NaeI GCCGGC 1 cut(s) 268
NarI GGCGCC 1 cut(s) 270
NciI CCSGG 1 cut(s) 223
NdeII GATC 3 cut(s) 567, 612, 684
NgoMIV GCCGGC 1 cut(s) 266
NlaIII CATG 3 cut(s) 239, 661, 730
NlaIV GGNNCC 8 cut(s) 227, 247, 271, 432, 480, 543, 544, 676
NmuCI GTSAC 1 cut(s) 505
PciSI GCTCTTC 1 cut(s) 374
PdiI GCCGGC 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 861
PfeI GAWTC 2 cut(s) 669, 857
Pfl23II CGTACG 1 cut(s) 82
PkrI GCNGC 4 cut(s) 211, 366, 919, 922
PleI GAGTC 1 cut(s) 604
PluTI GGCGCC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 604
PpuMI RGGWCCY 1 cut(s) 542
Psp5II RGGWCCY 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 380
PspFI CCCAGC 1 cut(s) 713
PspGI CCWGG 1 cut(s) 380
PspLI CGTACG 1 cut(s) 82
PspN4I GGNNCC 8 cut(s) 227, 247, 271, 432, 480, 543, 544, 676
PspPI GGNCC 4 cut(s) 346, 478, 542, 675
PspPPI RGGWCCY 1 cut(s) 542
PsuI RGATCY 1 cut(s) 684
RsaI GTAC 2 cut(s) 84, 261
RsaNI GTAC 2 cut(s) 83, 260
RseI CAYNNNNRTG 1 cut(s) 725
SapI GCTCTTC 1 cut(s) 374
SaqAI TTAA 2 cut(s) 36, 441
SatI GCNGC 4 cut(s) 210, 365, 918, 921
Sau3AI GATC 3 cut(s) 567, 612, 684
Sau96I GGNCC 4 cut(s) 346, 478, 542, 675
SchI GAGTC 1 cut(s) 605
ScrFI CCNGG 2 cut(s) 223, 382
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 2 cut(s) 229, 750
SfcI CTRYAG 1 cut(s) 846
SfoI GGCGCC 1 cut(s) 271
SgrAI CRCCGGYG 1 cut(s) 266
SinI GGWCC 4 cut(s) 346, 478, 542, 675
SmiMI CAYNNNNRTG 1 cut(s) 725
SmlI CTYRAG 1 cut(s) 448
SmoI CTYRAG 1 cut(s) 448
SpeI ACTAGT 1 cut(s) 493
Sse9I AATT 4 cut(s) 341, 387, 777, 793
SsiI CCGC 7 cut(s) 149, 166, 198, 210, 219, 252, 921
SspDI GGCGCC 1 cut(s) 269
SspMI CTAG 2 cut(s) 494, 581
StyD4I CCNGG 2 cut(s) 221, 380
StyI CCWWGG 2 cut(s) 474, 754
TaaI ACNGT 2 cut(s) 814, 850
TaiI ACGT 1 cut(s) 401
TaqI TCGA 1 cut(s) 294
TasI AATT 4 cut(s) 341, 387, 777, 793
TauI GCSGC 2 cut(s) 212, 923
TfiI GAWTC 2 cut(s) 669, 857
Tru1I TTAA 2 cut(s) 36, 441
Tru9I TTAA 2 cut(s) 36, 441
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 2 cut(s) 364, 917
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 1 cut(s) 501
TspGWI ACGGA 1 cut(s) 493
VpaK11BI GGWCC 4 cut(s) 346, 478, 542, 675
XcmI CCANNNNNNNNNTGG 1 cut(s) 356
XmnI GAANNNNTTC 1 cut(s) 861
XspI CTAG 2 cut(s) 494, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.