Rroxscaffold_5G00349380

5'-nucleotidase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0019526
Physical Location & Seq
Forward (+)
22286194 .. 22321925
35732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00349380.1

Sequence Viewer

Length: 744 bp
ATGGCTGAAAGTTTGTTTCTTTGGGATGAAATACGTATACAAAATGAGGATGCTTACCGGGTGGAGCAGGCAAAATTTCATATATTCCAAGAATTGCTTGGTAGACTTCATGCAACGGTAGCTAATAGTCAGAAAAGTGAGGCCAAGTTACTGTTGGACGAGCTAAATGAGTGCAGGCGTAAGGCAAGCTACAAGATGAAGAGAATGTTCAATAGCTCTTTCGGAGCCACCTTCCTTACGGATAAAGGTCAAGAATCTTCTTTCGCGTATCATATTCATCAATATGCAGATGTCTATACCAGTAAGCCAGAGAATTTTTTGCTCTATCCACCTGAAGCATGGCTTCATGCACCCTACGATATTAAGATTATGCCACATCATGTGAAGCCTATGGAGTTTTCAAATCTTATAGATTTTGGAGAAGCTAGGGGCAAAATGATCCTTGTCAAAAACCGAACCTTCGACGCCGGTTTGGTAGCCGGATATGGCCGGAATTGCCGGAAATCGACGAAACCTCCTTTCGGCTATAGTAATCTTCGCGGCTTAATTCCGAGCTCCTCCGCCAAGACACCGCAAAACACCACCACGGGCGTGACAAGGAGGAGGAGGCGAGCTTGCGGTGCCCGCATTCCGTCGTGGGTCGGTCGGAGAAAGGAGAAATCGGAGGAAGAAGAAACCGGACGAAGAGGAAGAAAGGGTCGGGGAGAGGAGAGAGAAAAACCGCTTGTGACGGCTTGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

247

Amino Acids

28.41

Weight (kDa)

9.65

Isoelectric Point (pI)

59.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 7 - 128 1.8e-19 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 620
AccI GTMKAC 2 cut(s) 37, 103
AccII CGCG 2 cut(s) 266, 540
AciI CCGC 6 cut(s) 540, 561, 572, 618, 625, 722
AclWI GGATC 1 cut(s) 433
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 2 cut(s) 74, 313
AcuI CTGAAG 1 cut(s) 354
AcyI GRCGYC 1 cut(s) 465
AfiI CCNNNNNNNGG 1 cut(s) 521
AgsI TTSAA 2 cut(s) 211, 402
AleI CACNNNNGTG 1 cut(s) 590
AluBI AGCT 7 cut(s) 122, 163, 189, 216, 425, 555, 614
AluI AGCT 7 cut(s) 122, 163, 189, 216, 425, 555, 614
Alw21I GWGCWC 1 cut(s) 557
AlwI GGATC 1 cut(s) 433
AoxI GGCC 2 cut(s) 141, 487
ApoI RAATTY 2 cut(s) 74, 313
AsuC2I CCSGG 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 625
BanI GGYRCC 1 cut(s) 620
BanII GRGCYC 1 cut(s) 557
Bbv12I GWGCWC 1 cut(s) 557
BcnI CCSGG 1 cut(s) 59
BfaI CTAG 1 cut(s) 426
BfmI CTRYAG 1 cut(s) 526
BisI GCNGC 1 cut(s) 541
BlsI GCNGC 1 cut(s) 542
Bme1390I CCNGG 1 cut(s) 59
BmiI GGNNCC 2 cut(s) 226, 622
BmrFI CCNGG 1 cut(s) 59
BmsI GCATC 1 cut(s) 40
BpuMI CCSGG 1 cut(s) 59
BsaAI YACGTR 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 465
BsaJI CCNNGG 1 cut(s) 585
BsaWI WCCGGW 1 cut(s) 677
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bsc4I CCNNNNNNNGG 1 cut(s) 521
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 585
BseGI GGATG 2 cut(s) 31, 55
BseLI CCNNNNNNNGG 1 cut(s) 521
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 4 cut(s) 547, 616, 619, 722
BseSI GKGCMC 1 cut(s) 625
BsgI GTGCAG 1 cut(s) 193
Bsh1236I CGCG 2 cut(s) 266, 540
Bsh1285I CGRYCG 1 cut(s) 646
BshFI GGCC 2 cut(s) 143, 489
BshNI GGYRCC 1 cut(s) 620
BsiEI CGRYCG 1 cut(s) 646
BsiHKAI GWGCWC 1 cut(s) 557
BsiSI CCGG 6 cut(s) 58, 468, 480, 490, 499, 678
BslI CCNNNNNNNGG 1 cut(s) 521
BsmI GAATGC 1 cut(s) 627
BsnI GGCC 2 cut(s) 143, 489
Bsp1286I GDGCHC 2 cut(s) 557, 625
Bsp143I GATC 1 cut(s) 438
BspACI CCGC 6 cut(s) 540, 561, 572, 618, 625, 722
BspANI GGCC 2 cut(s) 143, 489
BspFNI CGCG 2 cut(s) 266, 540
BspLI GGNNCC 2 cut(s) 226, 622
BspPI GGATC 1 cut(s) 433
BspT107I GGYRCC 1 cut(s) 620
BsrFI RCCGGY 1 cut(s) 467
BsrI ACTGG 1 cut(s) 300
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 1 cut(s) 585
BssMI GATC 1 cut(s) 438
BssNAI GTATAC 1 cut(s) 38
BssNI GRCGYC 1 cut(s) 465
Bst1107I GTATAC 1 cut(s) 38
Bst4CI ACNGT 2 cut(s) 118, 153
Bst6I CTCTTC 2 cut(s) 194, 679
BstACI GRCGYC 1 cut(s) 465
BstBAI YACGTR 1 cut(s) 35
BstC8I GCNNGC 6 cut(s) 69, 176, 187, 612, 616, 625
BstDSI CCRYGG 1 cut(s) 585
BstF5I GGATG 2 cut(s) 31, 55
BstFNI CGCG 2 cut(s) 266, 540
BstKTI GATC 1 cut(s) 441
BstMBI GATC 1 cut(s) 438
BstMCI CGRYCG 1 cut(s) 646
BstMWI GCNNNNNNNGC 4 cut(s) 119, 495, 620, 624
BstSCI CCNGG 1 cut(s) 57
BstSFI CTRYAG 1 cut(s) 526
BstSLI GKGCMC 1 cut(s) 625
BstSNI TACGTA 1 cut(s) 35
BstUI CGCG 2 cut(s) 266, 540
BstZ17I GTATAC 1 cut(s) 38
BsuRI GGCC 2 cut(s) 143, 489
BtgI CCRYGG 1 cut(s) 585
BtsCI GGATG 2 cut(s) 31, 55
Cac8I GCNNGC 6 cut(s) 69, 176, 187, 612, 616, 625
Cfr10I RCCGGY 1 cut(s) 467
CseI GACGC 1 cut(s) 473
CviAII CATG 4 cut(s) 110, 339, 347, 380
DpnI GATC 1 cut(s) 440
DpnII GATC 1 cut(s) 438
EaeI YGGCCR 1 cut(s) 487
Eam1104I CTCTTC 2 cut(s) 194, 679
EarI CTCTTC 2 cut(s) 194, 679
EciI GGCGGA 1 cut(s) 550
Ecl136II GAGCTC 1 cut(s) 555
Eco105I TACGTA 1 cut(s) 35
Eco24I GRGCYC 1 cut(s) 557
Eco53kI GAGCTC 1 cut(s) 555
Eco57I CTGAAG 1 cut(s) 354
EcoICRI GAGCTC 1 cut(s) 555
EcoT38I GRGCYC 1 cut(s) 557
FaeI CATG 4 cut(s) 113, 342, 350, 383
FalI AAGNNNNNCTT 4 cut(s) 81, 113, 327, 359
FatI CATG 4 cut(s) 109, 338, 346, 379
FauI CCCGC 1 cut(s) 632
FblI GTMKAC 2 cut(s) 37, 103
Fnu4HI GCNGC 1 cut(s) 541
FokI GGATG 2 cut(s) 38, 62
FriOI GRGCYC 1 cut(s) 557
Fsp4HI GCNGC 1 cut(s) 541
FspBI CTAG 1 cut(s) 426
GluI GCNGC 1 cut(s) 541
HaeIII GGCC 2 cut(s) 143, 489
HapII CCGG 6 cut(s) 58, 468, 480, 490, 499, 678
HgaI GACGC 1 cut(s) 473
Hin1I GRCGYC 1 cut(s) 465
Hin1II CATG 4 cut(s) 113, 342, 350, 383
HinfI GANTC 1 cut(s) 254
HpaII CCGG 6 cut(s) 58, 468, 480, 490, 499, 678
Hpy166II GTNNAC 2 cut(s) 38, 104
Hpy188I TCNGA 5 cut(s) 132, 224, 552, 648, 664
Hpy188III TCNNGA 1 cut(s) 251
Hpy8I GTNNAC 2 cut(s) 38, 104
Hpy99I CGWCG 3 cut(s) 467, 511, 637
HpyAV CCTTC 2 cut(s) 241, 469
HpyCH4III ACNGT 2 cut(s) 118, 153
HpyCH4IV ACGT 1 cut(s) 34
HpyCH4V TGCA 4 cut(s) 113, 174, 287, 350
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 495, 620, 624
HpySE526I ACGT 1 cut(s) 34
Hsp92I GRCGYC 1 cut(s) 465
Hsp92II CATG 4 cut(s) 113, 342, 350, 383
Kzo9I GATC 1 cut(s) 438
LmnI GCTCC 3 cut(s) 64, 224, 560
LweI GCATC 1 cut(s) 40
MaeI CTAG 1 cut(s) 426
MaeII ACGT 1 cut(s) 34
MaeIII GTNAC 3 cut(s) 147, 592, 727
MalI GATC 1 cut(s) 440
MboI GATC 1 cut(s) 438
MboII GAAGA 7 cut(s) 211, 249, 527, 680, 683, 696, 702
MhlI GDGCHC 2 cut(s) 557, 625
MluCI AATT 5 cut(s) 74, 92, 313, 493, 546
MmeI TCCRAC 2 cut(s) 135, 626
MseI TTAA 2 cut(s) 363, 545
MslI CAYNNNNRTG 2 cut(s) 282, 590
MspI CCGG 6 cut(s) 58, 468, 480, 490, 499, 678
MspR9I CCNGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 627
MvnI CGCG 2 cut(s) 266, 540
MwoI GCNNNNNNNGC 4 cut(s) 119, 495, 620, 624
NciI CCSGG 1 cut(s) 59
NdeII GATC 1 cut(s) 438
NlaIII CATG 4 cut(s) 113, 342, 350, 383
NlaIV GGNNCC 2 cut(s) 226, 622
NmuCI GTSAC 2 cut(s) 592, 727
OliI CACNNNNGTG 1 cut(s) 590
PctI GAATGC 1 cut(s) 627
PfeI GAWTC 1 cut(s) 254
PkrI GCNGC 1 cut(s) 542
Ppu21I YACGTR 1 cut(s) 35
Psp124BI GAGCTC 1 cut(s) 557
PspN4I GGNNCC 2 cut(s) 226, 622
RseI CAYNNNNRTG 2 cut(s) 282, 590
SacI GAGCTC 1 cut(s) 557
SaqAI TTAA 2 cut(s) 363, 545
SatI GCNGC 1 cut(s) 541
Sau3AI GATC 1 cut(s) 438
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 2 cut(s) 557, 625
SfaNI GCATC 1 cut(s) 40
SfcI CTRYAG 1 cut(s) 526
SmiMI CAYNNNNRTG 2 cut(s) 282, 590
SnaBI TACGTA 1 cut(s) 35
Sse9I AATT 5 cut(s) 74, 92, 313, 493, 546
SsiI CCGC 6 cut(s) 540, 561, 572, 618, 625, 722
SspMI CTAG 1 cut(s) 426
SstI GAGCTC 1 cut(s) 557
StyD4I CCNGG 1 cut(s) 57
TaaI ACNGT 2 cut(s) 118, 153
TaiI ACGT 1 cut(s) 37
TaqI TCGA 2 cut(s) 462, 506
TaqII GACCGA 1 cut(s) 632
TasI AATT 5 cut(s) 74, 92, 313, 493, 546
TauI GCSGC 1 cut(s) 543
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 2 cut(s) 363, 545
Tru9I TTAA 2 cut(s) 363, 545
TseFI GTSAC 2 cut(s) 592, 727
Tsp45I GTSAC 2 cut(s) 592, 727
TspDTI ATGAA 6 cut(s) 42, 68, 98, 212, 266, 335
TspGWI ACGGA 2 cut(s) 254, 621
XapI RAATTY 2 cut(s) 74, 313
XcmI CCANNNNNNNNNTGG 3 cut(s) 95, 151, 336
XmiI GTMKAC 2 cut(s) 37, 103
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.