Rroxscaffold_7G00171370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0019526
Physical Location & Seq
Forward (+)
11700185 .. 11700863
679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00171370.1

Sequence Viewer

Length: 414 bp
ATGGATCACGAAATGGGTGAAGCTATAACCCAAACCCAAGTATTCTTATTTTTGGGCTGCGGTATCGCCCGCCGTGCTAAATCCCCTAAAGGATCTTGCCAAAAATACTTTTGGGCTCAAACATTGGCCCCTGTGCCTCGAAATTGGGCTCGCGAAAAGATCTTAACAAGAAAGCCGCCCACACTGGGGAACCAGCGTAGGTGCCCGCCGTTTCATCCATACCATCTTTCAAAGACATCTCGCTTCTCGTACTCCCAAACCAACATGTCAAGTTGGGCCCTTCACCGCGAGATTCCATCCAGGACGATTCACTCGCCCCTACGGCCTGCCCATCCGACAACCCATTCCGGTCCTCGAGAAGAACAACGGGAGATTTCACTAATTGGGGTGCGCATCGCACGAGAGCCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.77

Weight (kDa)

10.7

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 201
AccII CGCG 2 cut(s) 153, 288
AciI CCGC 5 cut(s) 60, 70, 176, 206, 286
AclWI GGATC 2 cut(s) 12, 100
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 2 cut(s) 185, 186
AflIII ACRYGT 1 cut(s) 264
AgsI TTSAA 1 cut(s) 231
AjnI CCWGG 1 cut(s) 299
AluBI AGCT 1 cut(s) 23
AluI AGCT 1 cut(s) 23
AlwI GGATC 2 cut(s) 12, 100
Ama87I CYCGRG 1 cut(s) 354
AoxI GGCC 3 cut(s) 126, 276, 323
ApaI GGGCCC 1 cut(s) 280
ApeKI GCWGC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 393
AspS9I GGNCC 4 cut(s) 127, 276, 277, 350
AsuHPI GGTGA 2 cut(s) 29, 275
AvaI CYCGRG 1 cut(s) 354
AvaII GGWCC 1 cut(s) 350
BaeGI GKGCMC 2 cut(s) 206, 280
BanI GGYRCC 1 cut(s) 201
BanII GRGCYC 3 cut(s) 118, 151, 280
BauI CACGAG 1 cut(s) 399
BbvI GCAGC 1 cut(s) 44
BccI CCATC 3 cut(s) 231, 304, 339
BceAI ACGGC 3 cut(s) 57, 193, 338
BciT130I CCWGG 1 cut(s) 301
BglI GCCNNNNNGGC 1 cut(s) 322
BglII AGATCT 1 cut(s) 159
BisI GCNGC 2 cut(s) 58, 176
BlsI GCNGC 2 cut(s) 59, 177
Bme1390I CCNGG 1 cut(s) 301
Bme18I GGWCC 1 cut(s) 350
BmeT110I CYCGRG 1 cut(s) 354
BmgT120I GGNCC 4 cut(s) 127, 276, 277, 350
BmiI GGNNCC 4 cut(s) 129, 191, 203, 278
BmrFI CCNGG 1 cut(s) 301
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 1 cut(s) 402
BmuI ACTGGG 1 cut(s) 194
BsaWI WCCGGW 1 cut(s) 347
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 186
Bse1I ACTGG 1 cut(s) 189
BseBI CCWGG 1 cut(s) 301
BseGI GGATG 3 cut(s) 214, 296, 331
BseLI CCNNNNNNNGG 2 cut(s) 185, 186
BseNI ACTGG 1 cut(s) 189
BseSI GKGCMC 2 cut(s) 206, 280
BseXI GCAGC 1 cut(s) 44
Bsh1236I CGCG 2 cut(s) 153, 288
BshFI GGCC 3 cut(s) 128, 278, 325
BshNI GGYRCC 1 cut(s) 201
BsiHKCI CYCGRG 1 cut(s) 354
BsiSI CCGG 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 185, 186
BsnI GGCC 3 cut(s) 128, 278, 325
BsoBI CYCGRG 1 cut(s) 354
Bsp120I GGGCCC 1 cut(s) 276
Bsp1286I GDGCHC 4 cut(s) 118, 151, 206, 280
Bsp143I GATC 3 cut(s) 4, 92, 159
Bsp68I TCGCGA 1 cut(s) 153
BspACI CCGC 5 cut(s) 60, 70, 176, 206, 286
BspANI GGCC 3 cut(s) 128, 278, 325
BspFNI CGCG 2 cut(s) 153, 288
BspLI GGNNCC 4 cut(s) 129, 191, 203, 278
BspPI GGATC 2 cut(s) 12, 100
BspT107I GGYRCC 1 cut(s) 201
BsrI ACTGG 1 cut(s) 189
BssMI GATC 3 cut(s) 4, 92, 159
BssSI CACGAG 1 cut(s) 399
Bst2BI CACGAG 1 cut(s) 399
Bst2UI CCWGG 1 cut(s) 301
BstC8I GCNNGC 4 cut(s) 70, 151, 206, 327
BstF5I GGATG 3 cut(s) 214, 296, 331
BstFNI CGCG 2 cut(s) 153, 288
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 3 cut(s) 7, 95, 162
BstMBI GATC 3 cut(s) 4, 92, 159
BstMWI GCNNNNNNNGC 2 cut(s) 74, 322
BstNI CCWGG 1 cut(s) 301
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 299
BstSLI GKGCMC 2 cut(s) 206, 280
BstUI CGCG 2 cut(s) 153, 288
BstV1I GCAGC 1 cut(s) 44
BstX2I RGATCY 2 cut(s) 92, 159
BstYI RGATCY 2 cut(s) 92, 159
BsuRI GGCC 3 cut(s) 128, 278, 325
BtgZI GCGATG 1 cut(s) 379
BtsCI GGATG 3 cut(s) 214, 296, 331
BtsIMutI CAGTG 1 cut(s) 182
BtuMI TCGCGA 1 cut(s) 153
Cac8I GCNNGC 4 cut(s) 70, 151, 206, 327
CfoI GCGC 1 cut(s) 393
Cfr13I GGNCC 4 cut(s) 127, 276, 277, 350
Csp6I GTAC 1 cut(s) 250
CviAII CATG 1 cut(s) 265
CviJI RGCY 9 cut(s) 23, 57, 116, 128, 149, 175, 278, 325, 406
CviKI_1 RGCY 9 cut(s) 23, 57, 116, 128, 149, 175, 278, 325, 406
CviQI GTAC 1 cut(s) 250
DpnI GATC 3 cut(s) 6, 94, 161
DpnII GATC 3 cut(s) 4, 92, 159
Eco24I GRGCYC 3 cut(s) 118, 151, 280
Eco47I GGWCC 1 cut(s) 350
Eco88I CYCGRG 1 cut(s) 354
EcoO109I RGGNCCY 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 299
EcoT38I GRGCYC 3 cut(s) 118, 151, 280
FaeI CATG 1 cut(s) 268
FaiI YATR 3 cut(s) 26, 220, 266
FatI CATG 1 cut(s) 264
FauI CCCGC 2 cut(s) 77, 213
Fnu4HI GCNGC 2 cut(s) 58, 176
FokI GGATG 3 cut(s) 201, 283, 318
FriOI GRGCYC 3 cut(s) 118, 151, 280
Fsp4HI GCNGC 2 cut(s) 58, 176
FspAI RTGCGCAY 1 cut(s) 392
FspI TGCGCA 1 cut(s) 392
GlaI GCGC 1 cut(s) 392
GluI GCNGC 2 cut(s) 58, 176
HaeIII GGCC 3 cut(s) 128, 278, 325
HapII CCGG 1 cut(s) 348
HhaI GCGC 1 cut(s) 393
Hin1II CATG 1 cut(s) 268
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HinfI GANTC 2 cut(s) 292, 307
HpaII CCGG 1 cut(s) 348
HphI GGTGA 2 cut(s) 29, 275
Hpy188I TCNGA 1 cut(s) 336
Hpy188III TCNNGA 3 cut(s) 8, 152, 356
HpyAV CCTTC 1 cut(s) 290
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 322
Hsp92II CATG 1 cut(s) 268
HspAI GCGC 1 cut(s) 391
Kzo9I GATC 3 cut(s) 4, 92, 159
LpnPI CCDG 7 cut(s) 144, 170, 206, 286, 313, 339, 361
Lsp1109I GCAGC 1 cut(s) 44
LweI GCATC 1 cut(s) 402
MalI GATC 3 cut(s) 6, 94, 161
MboI GATC 3 cut(s) 4, 92, 159
MboII GAAGA 1 cut(s) 371
MflI RGATCY 2 cut(s) 92, 159
MhlI GDGCHC 4 cut(s) 118, 151, 206, 280
MluCI AATT 2 cut(s) 142, 381
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 2 cut(s) 147, 363
MseI TTAA 1 cut(s) 164
MspI CCGG 1 cut(s) 348
MspR9I CCNGG 1 cut(s) 301
MvaI CCWGG 1 cut(s) 301
MvnI CGCG 2 cut(s) 153, 288
MwoI GCNNNNNNNGC 2 cut(s) 74, 322
NdeII GATC 3 cut(s) 4, 92, 159
NlaIII CATG 1 cut(s) 268
NlaIV GGNNCC 4 cut(s) 129, 191, 203, 278
NruI TCGCGA 1 cut(s) 153
NsbI TGCGCA 1 cut(s) 392
NspI RCATGY 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 354
PciI ACATGT 1 cut(s) 264
PfeI GAWTC 2 cut(s) 292, 307
PfoI TCCNGGA 1 cut(s) 299
PkrI GCNGC 2 cut(s) 59, 177
PscI ACATGT 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 4 cut(s) 129, 191, 203, 278
PspOMI GGGCCC 1 cut(s) 276
PspPI GGNCC 4 cut(s) 127, 276, 277, 350
PsuI RGATCY 2 cut(s) 92, 159
RruI TCGCGA 1 cut(s) 153
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SaqAI TTAA 1 cut(s) 164
SatI GCNGC 2 cut(s) 58, 176
Sau3AI GATC 3 cut(s) 4, 92, 159
Sau96I GGNCC 4 cut(s) 127, 276, 277, 350
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 4 cut(s) 118, 151, 206, 280
SetI ASST 2 cut(s) 25, 203
SfaNI GCATC 1 cut(s) 402
Sfr274I CTCGAG 1 cut(s) 354
SinI GGWCC 1 cut(s) 350
SlaI CTCGAG 1 cut(s) 354
SmlI CTYRAG 1 cut(s) 354
SmoI CTYRAG 1 cut(s) 354
Sse9I AATT 2 cut(s) 142, 381
SsiI CCGC 5 cut(s) 60, 70, 176, 206, 286
StyD4I CCNGG 1 cut(s) 299
TaqI TCGA 2 cut(s) 139, 355
TasI AATT 2 cut(s) 142, 381
TauI GCSGC 1 cut(s) 178
TfiI GAWTC 2 cut(s) 292, 307
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TscAI CASTG 1 cut(s) 189
TseI GCWGC 1 cut(s) 57
TspDTI ATGAA 1 cut(s) 203
TspRI CASTG 1 cut(s) 189
VpaK11BI GGWCC 1 cut(s) 350
XceI RCATGY 1 cut(s) 268
XhoI CTCGAG 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.