Rroxscaffold_5G00387070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012945
Physical Location & Seq
Reverse (-)
65820639 .. 65822033
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00387070.1

Sequence Viewer

Length: 993 bp
ATGGCCAAGGTCCCTGAGAAAACAAGCCCTCCGTTAAAACCCAAAACCACTACCTTTCTCGGTTGTTTCAGATTTTCTAGTAGAAAAACCCTCCGATCCCATGACTATAACAACAACAAGATCATTAAGAGTCGCAGCTGGTTTTGTTGGTCAAGGTTTCGCTCGAAGAAGGCGGCCGCCACAAAAACGGTGGTGCCCGTCGACATCTCCACCGTATCCGAGAAACAAGCAATCCCGTCCACGTTGGTCAAGTTGGAGCCGAAGTCAAAGCACGGTAAGTTTGAACCCAAAAACGAAGTTCCGGCAGCAACCTACGTCGTGGAGGGCCCAGGTGTTCCAATTCAGCCTCTGATAGTCTCCGAAAAAGCTCCTAATTCGAAACAAAAACCTCCAGAGACAGCCTCGGACATGATCAGCCTCGAAAACAGCACACAGGGCTCAGATGCTTCCAAAGACTACACGTATCAAAAGCGGCGGCTGTCTTTTCGCCAGAAAGTTGAATCTATAAGAACAGCCGCAGCTGGGGGAGCCGGCAGCAGCCAACCCGGCTCACCGGTTCAACAGGAGAACAAATCAAGGGGAACAGTCCCCGTGATCTGTCCCCAGGCAACATCGCTTCCCGTCACTCCGAAACGTCCAAGACGAGGAGGCGGAGGAGGAGGAGGGTCTTCAGCTATTCCGTCGTCGGCACGTAAAACATGGTTGGTGTCAAATGAGCCGACTATGACCACGAAGAGATTGGACCCGCTGGTGGGTATGACTATTATTATGGTCACATTGATTATAATGGTATTATGGGGCCGATTATGCGCCATTCTCTGCACCGCCGCATGGTTCTATCTCCTCCCTCGCTTAACTCAAAATAGTAATTTAACAACAACATCCTCCACCGCCGGCGATGATCATTTGGACTTCAGTTCCGACGAATACAAGAAGAAGGTGGTCTTAGAGGGATTCCTCGAAAGGAATAACCACCGCAATCATGTACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

330

Amino Acids

36.19

Weight (kDa)

10.04

Isoelectric Point (pI)

52.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012945)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08240 AT5G23160
fragaria_vesca FvH4_4g36960
malus_domestica MD13G1005200.v1.1 MD16G1002700.v1.1
prunus_persica Prupe.1G347600_v2.0.a1
pyrus_communis pycom13g00440 pycom16g00320
rosa_chinensis RchiOBHm_Chr4g0446731
rosa_laevigata RLG00000005635
rosa_multiflora Rmu_sc0001753.1_g000035
rosa_roxburghii Rroxscaffold_5G00387070
rosa_rugosa Rorug04G0374300
rosa_samantha Rh4AG435700 Rh4BG444500 Rh4CG461800 Rh4DG443500
rosa_wichuraiana Rw4G037240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 785
AccB1I GGYRCC 1 cut(s) 193
AccI GTMKAC 1 cut(s) 201
AclWI GGATC 1 cut(s) 90
AcoI YGGCCR 2 cut(s) 3, 174
AcuI CTGAAG 2 cut(s) 654, 898
AfaI GTAC 1 cut(s) 987
AfiI CCNNNNNNNGG 5 cut(s) 522, 644, 751, 752, 831
AflIII ACRYGT 1 cut(s) 459
AgeI ACCGGT 1 cut(s) 553
AgsI TTSAA 3 cut(s) 284, 500, 560
AjnI CCWGG 2 cut(s) 328, 603
AluBI AGCT 4 cut(s) 138, 368, 521, 674
AluI AGCT 4 cut(s) 138, 368, 521, 674
Alw26I GTCTC 2 cut(s) 361, 389
AlwI GGATC 1 cut(s) 90
AlwNI CAGNNNCTG 1 cut(s) 349
AoxI GGCC 4 cut(s) 3, 174, 325, 799
ApaI GGGCCC 1 cut(s) 329
ApeKI GCWGC 5 cut(s) 135, 305, 518, 534, 537
AsiGI ACCGGT 1 cut(s) 553
AspLEI GCGC 1 cut(s) 812
AspS9I GGNCC 5 cut(s) 10, 325, 326, 742, 799
AsuC2I CCSGG 1 cut(s) 546
AsuHPI GGTGA 1 cut(s) 543
AsuII TTCGAA 1 cut(s) 377
AvaII GGWCC 2 cut(s) 10, 742
BaeGI GKGCMC 2 cut(s) 198, 329
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 193
BanII GRGCYC 2 cut(s) 329, 440
BbsI GAAGAC 1 cut(s) 660
BbvI GCAGC 5 cut(s) 147, 317, 530, 546, 549
BciT130I CCWGG 2 cut(s) 330, 605
BciVI GTATCC 1 cut(s) 226
BclI TGATCA 2 cut(s) 411, 901
BcnI CCSGG 1 cut(s) 546
BcoDI GTCTC 2 cut(s) 361, 389
BfaI CTAG 1 cut(s) 78
BfuI GTATCC 1 cut(s) 226
BglI GCCNNNNNGGC 1 cut(s) 546
Bme1390I CCNGG 3 cut(s) 330, 546, 605
Bme18I GGWCC 2 cut(s) 10, 742
BmgT120I GGNCC 5 cut(s) 10, 325, 326, 742, 799
BmiI GGNNCC 7 cut(s) 12, 195, 258, 327, 529, 744, 800
BmrFI CCNGG 3 cut(s) 330, 546, 605
BmsI GCATC 1 cut(s) 433
BpiI GAAGAC 1 cut(s) 660
BplI GAGNNNNNCTC 2 cut(s) 386, 418
BpmI CTGGAG 1 cut(s) 375
Bpu14I TTCGAA 1 cut(s) 377
BpuMI CCSGG 1 cut(s) 546
BsaAI YACGTR 2 cut(s) 462, 692
BsaJI CCNNGG 4 cut(s) 6, 328, 402, 603
BsaWI WCCGGW 1 cut(s) 553
BsaXI ACNNNNNCTCC 4 cut(s) 13, 43, 248, 278
Bsc4I CCNNNNNNNGG 5 cut(s) 522, 644, 751, 752, 831
Bse118I RCCGGY 3 cut(s) 530, 553, 893
BseBI CCWGG 2 cut(s) 330, 605
BseDI CCNNGG 4 cut(s) 6, 328, 402, 603
BseGI GGATG 1 cut(s) 881
BseLI CCNNNNNNNGG 5 cut(s) 522, 644, 751, 752, 831
BseMII CTCAG 2 cut(s) 6, 453
BseRI GAGGAG 5 cut(s) 660, 669, 672, 675, 833
BseSI GKGCMC 2 cut(s) 198, 329
BseX3I CGGCCG 1 cut(s) 174
BseXI GCAGC 5 cut(s) 147, 317, 530, 546, 549
BseYI CCCAGC 1 cut(s) 521
BsgI GTGCAG 1 cut(s) 805
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 4 cut(s) 5, 176, 327, 801
BshNI GGYRCC 1 cut(s) 193
BshTI ACCGGT 1 cut(s) 553
BsiEI CGRYCG 1 cut(s) 177
BsiSI CCGG 5 cut(s) 302, 531, 546, 554, 894
BslFI GGGAC 2 cut(s) 572, 585
BslI CCNNNNNNNGG 5 cut(s) 522, 644, 751, 752, 831
BsmAI GTCTC 2 cut(s) 361, 389
BsmFI GGGAC 2 cut(s) 572, 585
BsnI GGCC 4 cut(s) 5, 176, 327, 801
Bsp119I TTCGAA 1 cut(s) 377
Bsp120I GGGCCC 1 cut(s) 325
Bsp1286I GDGCHC 3 cut(s) 198, 329, 440
Bsp143I GATC 5 cut(s) 95, 120, 411, 594, 901
BspANI GGCC 4 cut(s) 5, 176, 327, 801
BspCNI CTCAG 2 cut(s) 7, 452
BspLI GGNNCC 7 cut(s) 12, 195, 258, 327, 529, 744, 800
BspPI GGATC 1 cut(s) 90
BspT104I TTCGAA 1 cut(s) 377
BspT107I GGYRCC 1 cut(s) 193
BsrFI RCCGGY 3 cut(s) 530, 553, 893
BssAI RCCGGY 3 cut(s) 530, 553, 893
BssECI CCNNGG 4 cut(s) 6, 328, 402, 603
BssMI GATC 5 cut(s) 95, 120, 411, 594, 901
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 330, 605
Bst4CI ACNGT 4 cut(s) 190, 214, 275, 586
Bst6I CTCTTC 1 cut(s) 728
BstBAI YACGTR 2 cut(s) 462, 692
BstBI TTCGAA 1 cut(s) 377
BstC8I GCNNGC 2 cut(s) 532, 895
BstDEI CTNAG 3 cut(s) 15, 439, 946
BstF5I GGATG 1 cut(s) 881
BstHHI GCGC 1 cut(s) 812
BstKTI GATC 5 cut(s) 98, 123, 414, 597, 904
BstMAI GTCTC 2 cut(s) 361, 389
BstMBI GATC 5 cut(s) 95, 120, 411, 594, 901
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 4 cut(s) 435, 527, 546, 807
BstNI CCWGG 2 cut(s) 330, 605
BstSCI CCNGG 3 cut(s) 328, 544, 603
BstSLI GKGCMC 2 cut(s) 198, 329
BstV1I GCAGC 5 cut(s) 147, 317, 530, 546, 549
BstV2I GAAGAC 1 cut(s) 660
BstZI CGGCCG 1 cut(s) 174
BsuI GTATCC 1 cut(s) 226
BsuRI GGCC 4 cut(s) 5, 176, 327, 801
BtgZI GCGATG 2 cut(s) 597, 912
BtsCI GGATG 1 cut(s) 881
Cac8I GCNNGC 2 cut(s) 532, 895
CaiI CAGNNNCTG 1 cut(s) 349
CciNI GCGGCCGC 1 cut(s) 174
CfoI GCGC 1 cut(s) 812
Cfr10I RCCGGY 3 cut(s) 530, 553, 893
Cfr13I GGNCC 5 cut(s) 10, 325, 326, 742, 799
Csp6I GTAC 1 cut(s) 986
CspAI ACCGGT 1 cut(s) 553
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CviAII CATG 5 cut(s) 101, 409, 699, 831, 983
CviQI GTAC 1 cut(s) 986
DdeI CTNAG 3 cut(s) 15, 439, 946
DpnI GATC 5 cut(s) 97, 122, 413, 596, 903
DpnII GATC 5 cut(s) 95, 120, 411, 594, 901
EaeI YGGCCR 2 cut(s) 3, 174
EagI CGGCCG 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 728
EarI CTCTTC 1 cut(s) 728
EciI GGCGGA 1 cut(s) 666
EclXI CGGCCG 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 2 cut(s) 329, 440
Eco47I GGWCC 2 cut(s) 10, 742
Eco52I CGGCCG 1 cut(s) 174
Eco57I CTGAAG 2 cut(s) 654, 898
EcoO109I RGGNCCY 2 cut(s) 10, 325
EcoRII CCWGG 2 cut(s) 328, 603
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 329, 440
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 5 cut(s) 104, 412, 702, 834, 986
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 2 cut(s) 572, 585
FatI CATG 5 cut(s) 100, 408, 698, 830, 982
FauI CCCGC 1 cut(s) 753
FbaI TGATCA 2 cut(s) 411, 901
FblI GTMKAC 1 cut(s) 201
FokI GGATG 1 cut(s) 868
FriOI GRGCYC 2 cut(s) 329, 440
FspBI CTAG 1 cut(s) 78
GlaI GCGC 1 cut(s) 811
GsaI CCCAGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 4 cut(s) 5, 176, 327, 801
HapII CCGG 5 cut(s) 302, 531, 546, 554, 894
HhaI GCGC 1 cut(s) 812
Hin1II CATG 5 cut(s) 104, 412, 702, 834, 986
Hin6I GCGC 1 cut(s) 810
HinP1I GCGC 1 cut(s) 810
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 3 cut(s) 130, 500, 954
HpaII CCGG 5 cut(s) 302, 531, 546, 554, 894
HphI GGTGA 1 cut(s) 543
Hpy166II GTNNAC 2 cut(s) 202, 240
Hpy188I TCNGA 9 cut(s) 71, 95, 220, 351, 361, 406, 442, 630, 922
Hpy188III TCNNGA 1 cut(s) 392
Hpy8I GTNNAC 2 cut(s) 202, 240
Hpy99I CGWCG 5 cut(s) 203, 320, 685, 688, 926
HpyAV CCTTC 2 cut(s) 163, 931
HpyCH4III ACNGT 4 cut(s) 190, 214, 275, 586
HpyCH4IV ACGT 5 cut(s) 242, 315, 461, 634, 691
HpyCH4V TGCA 1 cut(s) 822
HpyF10VI GCNNNNNNNGC 4 cut(s) 435, 527, 546, 807
HpyF3I CTNAG 3 cut(s) 15, 439, 946
HpySE526I ACGT 5 cut(s) 242, 315, 461, 634, 691
Hsp92II CATG 5 cut(s) 104, 412, 702, 834, 986
HspAI GCGC 1 cut(s) 810
KroI GCCGGC 2 cut(s) 530, 893
KroNI GCCGGC 2 cut(s) 532, 895
Ksp22I TGATCA 2 cut(s) 411, 901
Kzo9I GATC 5 cut(s) 95, 120, 411, 594, 901
LmnI GCTCC 3 cut(s) 256, 373, 527
Lsp1109I GCAGC 5 cut(s) 147, 317, 530, 546, 549
LweI GCATC 1 cut(s) 433
MaeI CTAG 1 cut(s) 78
MaeII ACGT 5 cut(s) 242, 315, 461, 634, 691
MaeIII GTNAC 2 cut(s) 622, 772
MalI GATC 5 cut(s) 97, 122, 413, 596, 903
MboI GATC 5 cut(s) 95, 120, 411, 594, 901
MboII GAAGA 4 cut(s) 178, 660, 745, 946
MhlI GDGCHC 3 cut(s) 198, 329, 440
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 339, 373, 868
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 139
MmeI TCCRAC 2 cut(s) 234, 945
Mox20I TGGCCA 1 cut(s) 5
MreI CGCCGGCG 1 cut(s) 893
MroNI GCCGGC 2 cut(s) 530, 893
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 35, 126, 854, 872
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 138, 521, 748
MspI CCGG 5 cut(s) 302, 531, 546, 554, 894
MspR9I CCNGG 3 cut(s) 330, 546, 605
MvaI CCWGG 2 cut(s) 330, 605
MwoI GCNNNNNNNGC 4 cut(s) 435, 527, 546, 807
NaeI GCCGGC 2 cut(s) 532, 895
NciI CCSGG 1 cut(s) 546
NdeII GATC 5 cut(s) 95, 120, 411, 594, 901
NgoMIV GCCGGC 2 cut(s) 530, 893
NlaIII CATG 5 cut(s) 104, 412, 702, 834, 986
NlaIV GGNNCC 7 cut(s) 12, 195, 258, 327, 529, 744, 800
NmuCI GTSAC 2 cut(s) 622, 772
NotI GCGGCCGC 1 cut(s) 174
NspV TTCGAA 1 cut(s) 377
PcsI WCGNNNNNNNCGW 1 cut(s) 640
PdiI GCCGGC 2 cut(s) 532, 895
PfeI GAWTC 2 cut(s) 500, 954
PinAI ACCGGT 1 cut(s) 553
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
Ppu21I YACGTR 2 cut(s) 462, 692
PpuMI RGGWCCY 1 cut(s) 10
PsiI TTATAA 1 cut(s) 785
Psp5II RGGWCCY 1 cut(s) 10
Psp6I CCWGG 2 cut(s) 328, 603
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 2 cut(s) 328, 603
PspN4I GGNNCC 7 cut(s) 12, 195, 258, 327, 529, 744, 800
PspOMI GGGCCC 1 cut(s) 325
PspPI GGNCC 5 cut(s) 10, 325, 326, 742, 799
PspPPI RGGWCCY 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 349
PvuII CAGCTG 2 cut(s) 138, 521
RsaI GTAC 1 cut(s) 987
RsaNI GTAC 1 cut(s) 986
SalI GTCGAC 1 cut(s) 200
SaqAI TTAA 4 cut(s) 35, 126, 854, 872
Sau3AI GATC 5 cut(s) 95, 120, 411, 594, 901
Sau96I GGNCC 5 cut(s) 10, 325, 326, 742, 799
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 3 cut(s) 330, 546, 605
SduI GDGCHC 3 cut(s) 198, 329, 440
SfaNI GCATC 1 cut(s) 433
SfuI TTCGAA 1 cut(s) 377
SgrAI CRCCGGYG 1 cut(s) 893
SinI GGWCC 2 cut(s) 10, 742
Sse9I AATT 3 cut(s) 339, 373, 868
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 3 cut(s) 328, 544, 603
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 4 cut(s) 190, 214, 275, 586
TaiI ACGT 5 cut(s) 245, 318, 464, 637, 694
TaqI TCGA 5 cut(s) 164, 201, 377, 420, 960
TasI AATT 3 cut(s) 339, 373, 868
TatI WGTACW 1 cut(s) 985
TauI GCSGC 6 cut(s) 176, 179, 475, 478, 518, 830
TfiI GAWTC 2 cut(s) 500, 954
Tru1I TTAA 4 cut(s) 35, 126, 854, 872
Tru9I TTAA 4 cut(s) 35, 126, 854, 872
TseFI GTSAC 2 cut(s) 622, 772
TseI GCWGC 5 cut(s) 135, 305, 518, 534, 537
Tsp45I GTSAC 2 cut(s) 622, 772
TspGWI ACGGA 2 cut(s) 21, 669
VpaK11BI GGWCC 2 cut(s) 10, 742
XcmI CCANNNNNNNNNTGG 2 cut(s) 187, 736
XmiI GTMKAC 1 cut(s) 201
XspI CTAG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.