Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0006133

Physical Location & Seq

Genomic Coordinates Reverse (-)

31757077 .. 31759806

Gene Structure 2730 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00409220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGTATCCGAGAGATGACGAGAGGTTTCCAAAACCTCCTACGTCAATTTCGGGTCAATTGGACCCGAAAATGCCAGAGCCGCTGCTCGGCGGGGTTGCGATGGATATAGAAGGGATGTCTGGTGGGAGTAGCGACTGTGTGGGAGTGCCGCAGCCGCTTCAGTGTCTGCAGGAGACTCCGGTGCCGCCGTTTCTGTCCAAGACGTTTGATTTGGTGGACGACCCGTCGCTGGATTTGATCATATCGTGGGGTTCTACTGGAAGAAGCTTCGTGGTTTGGGACCCTGTGGAGTTTTCGAGGCTCATCTTGCCTCGGAATTTCAAGCACAACAATTTCTCCAGCTTTGTCCGCCAGCTTAATACTTATGGTTTTCGCAAGATCGATACAGATAAGTGGGAGTTTGCAAATGAAGCTTTCCTGAGGGGCAAAAGACATTTGTTGAAGAACATCCAGAGGCGCAAGTCATCTCAGTCCTTGCAGATTGGGAGCTTTACTGGGCCTTCTGCTGAAGCAGGAAGGCCTGGACTCGAAGGTGAGATAGAGTCACTGAGGAACCAGAAGAGTCTGTTAATGCAGGAGGTCGTTGACTTGCAACAGCAGCAGCGAGGTACAGTTCACCATATGAAAGCAGTGAATGAGAGGCTGCAGTCTGCTGAGCAGAGACAGCAGCAGATGGTTTCTTTCTTGGCCAAGGTGCTTCAAAACCCAGCATTCTTAGCCAGGCTTCAACAGAAGAAAGAACAGAAAGGTATAGGCTCTTCCTCTGGGCAGAGGAAATTTGTTAAGGCGCGTGAGCGTGAACTAGGTAGATCTGATTCTTATGTGGAAGGGCAAATTGTGAAGTACGAACCTGCTTGGAGAAACCATACCATGTCGTCTGCAGTCCCAGATGTAAATCCAGTTCCTATGGAAAACTCTCCAGATCATGGTATGCCATTTCAATTTGAGAATATTGCATTTGATGGGTTAATGGTCTCGGATGAACTAGAAGTAACACCAGAGCAAGAGGGTGAAGGGGCATCACGCATGGTAACTGAAGATCCACTTTCCAAAGGCAAGAGTGTTGTGACCCAACAACAAGAGGTTAATCCCGAGCCTTACATCTCTTTCCAGGAGGATTTGATGAAGGAGAGGAGTACTTTTGAGGATCTCTCATTTCCAGGGATTGAGAACATGGTTAAACCAGATGATGTATGGAGTATGGGTTTTGATGCCAGTGCTGGTATGTCAAGTTCTAGCAGTGGGTTTTGGAGTAATCCTATCAACTGTGATATGCCAGAGATGGGAATGCCAGACGGATTGTTTGATATCTGGGACATAGGTGCACTGCAAGCAGCAGGAGGGTCGGGCATTGATAAGTGGCCAGCTGATGAATCTGCATTCGATGAGCATGCAAGTCAAGATGAACTACCAAAAGATGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009889 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HSF_DNA-bind

Protein Analysis

474

Amino Acids

52.96

Weight (kDa)

4.82

Isoelectric Point (pI)

59.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSF_DNA-bind	PF00447	64 - 153	1.2e-28	HSF-type DNA-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03720 AT5G03720
fragaria_vesca	FvH4_6g17890
malus_domestica	MD12G1017900.v1.1 MD14G1015900.v1.1
prunus_persica	Prupe.7G117200_v2.0.a1 Prupe.7G117200_v2.0.a1 Prupe.7G117200_v2.0.a1 Prupe.7G117200_v2.0.a1 Prupe.7G117200_v2.0.a1 Prupe.7G117200_v2.0.a1
pyrus_communis	pycom03g05130 pycom12g01490 pycom14g01370
rosa_chinensis	RchiOBHm_Chr3g0472131 RchiOBHm_Chr3g0472141
rosa_laevigata	RLG00000024102
rosa_multiflora	Rmu_sc0002532.1_g000026
rosa_roxburghii	Rroxscaffold_6G00409220
rosa_rugosa	Rorug03G0122500 Rorug03G0122600
rosa_samantha	Rh3AG174300 Rh3BG199700 Rh3CG190800 Rh3CG198200 Rh3DG174800
rosa_wichuraiana	Rw3G016650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	859
AccB1I	GGYRCC	1 cut(s)	181
AccB7I	CCANNNNNTGG	1 cut(s)	926
AccII	CGCG	1 cut(s)	790
AciI	CCGC	6 cut(s)	80, 90, 149, 155, 185, 349
AclWI	GGATC	2 cut(s)	1034, 1155
AcoI	YGGCCR	2 cut(s)	687, 1361
AcsI	RAATTY	2 cut(s)	316, 776
AcuI	CTGAAG	3 cut(s)	143, 528, 1056
AfaI	GTAC	3 cut(s)	610, 845, 1138
AfiI	CCNNNNNNNGG	4 cut(s)	86, 229, 926, 1283
AgsI	TTSAA	5 cut(s)	322, 442, 701, 728, 941
AhdI	GACNNNNNGTC	1 cut(s)	223
AjnI	CCWGG	4 cut(s)	520, 719, 1110, 1159
AluBI	AGCT	6 cut(s)	267, 342, 355, 413, 489, 1367
AluI	AGCT	6 cut(s)	267, 342, 355, 413, 489, 1367
Alw21I	GWGCWC	1 cut(s)	1327
Alw26I	GTCTC	3 cut(s)	167, 655, 979
Alw44I	GTGCAC	1 cut(s)	1323
AlwI	GGATC	2 cut(s)	1034, 1155
AlwNI	CAGNNNCTG	1 cut(s)	166
Ama87I	CYCGRG	1 cut(s)	1091
AoxI	GGCC	4 cut(s)	497, 518, 687, 1361
ApaLI	GTGCAC	1 cut(s)	1323
ApeKI	GCWGC	7 cut(s)	82, 151, 598, 601, 643, 667, 1334
ApoI	RAATTY	2 cut(s)	316, 776
ArsI	GACNNNNNNTTYG	4 cut(s)	193, 225, 1286, 1318
AspLEI	GCGC	2 cut(s)	459, 790
AspS9I	GGNCC	3 cut(s)	61, 280, 497
AsuHPI	GGTGA	3 cut(s)	545, 608, 1022
AvaI	CYCGRG	1 cut(s)	1091
AvaII	GGWCC	2 cut(s)	61, 280
AxyI	CCTNAGG	1 cut(s)	419
BaeGI	GKGCMC	1 cut(s)	1327
BalI	TGGCCA	2 cut(s)	689, 1363
BanI	GGYRCC	1 cut(s)	181
BarI	GAAGNNNNNNTAC	2 cut(s)	742, 774
Bbv12I	GWGCWC	1 cut(s)	1327
BbvI	GCAGC	7 cut(s)	69, 163, 610, 613, 630, 679, 1346
BccI	CCATC	4 cut(s)	94, 667, 956, 1276
BceAI	ACGGC	1 cut(s)	172
BcgI	CGANNNNNNTGC	4 cut(s)	1326, 1360, 1373, 1407
BciT130I	CCWGG	4 cut(s)	522, 721, 1112, 1161
BciVI	GTATCC	1 cut(s)	15
BclI	TGATCA	1 cut(s)	237
BcoDI	GTCTC	3 cut(s)	167, 655, 979
BfaI	CTAG	3 cut(s)	803, 986, 1236
BfmI	CTRYAG	3 cut(s)	167, 644, 879
BfuAI	ACCTGC	1 cut(s)	859
BfuI	GTATCC	1 cut(s)	15
BglII	AGATCT	1 cut(s)	809
BlpI	GCTNAGC	1 cut(s)	654
BmcAI	AGTACT	1 cut(s)	1138
Bme1390I	CCNGG	4 cut(s)	522, 721, 1112, 1161
Bme18I	GGWCC	2 cut(s)	61, 280
BmeRI	GACNNNNNGTC	1 cut(s)	223
BmeT110I	CYCGRG	1 cut(s)	1091
BmgT120I	GGNCC	3 cut(s)	61, 280, 497
BmiI	GGNNCC	5 cut(s)	63, 183, 281, 282, 554
BmrFI	CCNGG	4 cut(s)	522, 721, 1112, 1161
BmrI	ACTGGG	1 cut(s)	504
BmsI	GCATC	2 cut(s)	1028, 1201
BmuI	ACTGGG	1 cut(s)	504
BplI	GAGNNNNNCTC	2 cut(s)	1136, 1168
BpmI	CTGGAG	2 cut(s)	322, 903
Bpu1102I	GCTNAGC	1 cut(s)	654
Bsa29I	ATCGAT	1 cut(s)	381
BsaBI	GATNNNNATC	1 cut(s)	894
BsaI	GGTCTC	1 cut(s)	979
BsaJI	CCNNGG	3 cut(s)	311, 690, 1160
BsaWI	WCCGGW	1 cut(s)	178
BsaXI	ACNNNNNCTCC	4 cut(s)	320, 350, 1189, 1219
Bsc4I	CCNNNNNNNGG	4 cut(s)	86, 229, 926, 1283
Bse1I	ACTGG	4 cut(s)	262, 499, 899, 1215
Bse21I	CCTNAGG	1 cut(s)	419
Bse8I	GATNNNNATC	1 cut(s)	894
BseBI	CCWGG	4 cut(s)	522, 721, 1112, 1161
BseCI	ATCGAT	1 cut(s)	381
BseDI	CCNNGG	3 cut(s)	311, 690, 1160
BseGI	GGATG	3 cut(s)	120, 447, 985
BseJI	GATNNNNATC	1 cut(s)	894
BseLI	CCNNNNNNNGG	4 cut(s)	86, 229, 926, 1283
BseMII	CTCAG	4 cut(s)	410, 482, 539, 645
BseNI	ACTGG	4 cut(s)	262, 499, 899, 1215
BseRI	GAGGAG	1 cut(s)	1147
BseSI	GKGCMC	1 cut(s)	1327
BseXI	GCAGC	7 cut(s)	69, 163, 610, 613, 630, 679, 1346
BseYI	CCCAGC	1 cut(s)	706
Bsh1236I	CGCG	1 cut(s)	790
BshFI	GGCC	4 cut(s)	499, 520, 689, 1363
BshNI	GGYRCC	1 cut(s)	181
BshVI	ATCGAT	1 cut(s)	381
BsiHKAI	GWGCWC	1 cut(s)	1327
BsiHKCI	CYCGRG	1 cut(s)	1091
BsiSI	CCGG	1 cut(s)	179
BslFI	GGGAC	3 cut(s)	293, 869, 1328
BslI	CCNNNNNNNGG	4 cut(s)	86, 229, 926, 1283
BsmAI	GTCTC	3 cut(s)	167, 655, 979
BsmFI	GGGAC	3 cut(s)	293, 869, 1328
BsmI	GAATGC	3 cut(s)	710, 1293, 1379
BsnI	GGCC	4 cut(s)	499, 520, 689, 1363
Bso31I	GGTCTC	1 cut(s)	979
BsoBI	CYCGRG	1 cut(s)	1091
Bsp1286I	GDGCHC	1 cut(s)	1327
Bsp143I	GATC	6 cut(s)	237, 378, 809, 922, 1039, 1147
Bsp1720I	GCTNAGC	1 cut(s)	654
BspACI	CCGC	6 cut(s)	80, 90, 149, 155, 185, 349
BspANI	GGCC	4 cut(s)	499, 520, 689, 1363
BspCNI	CTCAG	4 cut(s)	411, 481, 540, 646
BspDI	ATCGAT	1 cut(s)	381
BspFNI	CGCG	1 cut(s)	790
BspLI	GGNNCC	5 cut(s)	63, 183, 281, 282, 554
BspMAI	CTGCAG	3 cut(s)	171, 648, 883
BspMI	ACCTGC	1 cut(s)	859
BspPI	GGATC	2 cut(s)	1034, 1155
BspQI	GCTCTTC	1 cut(s)	763
BspT107I	GGYRCC	1 cut(s)	181
BspTNI	GGTCTC	1 cut(s)	979
BsrI	ACTGG	4 cut(s)	262, 499, 899, 1215
BssECI	CCNNGG	3 cut(s)	311, 690, 1160
BssMI	GATC	6 cut(s)	237, 378, 809, 922, 1039, 1147
BssT1I	CCWWGG	1 cut(s)	690
Bst2UI	CCWGG	4 cut(s)	522, 721, 1112, 1161
Bst4CI	ACNGT	3 cut(s)	137, 613, 1268
Bst6I	CTCTTC	2 cut(s)	554, 763
BstC8I	GCNNGC	4 cut(s)	353, 1332, 1365, 1392
BstDEI	CTNAG	5 cut(s)	419, 468, 548, 654, 715
BstF5I	GGATG	3 cut(s)	120, 447, 985
BstFNI	CGCG	1 cut(s)	790
BstHHI	GCGC	2 cut(s)	459, 790
BstKTI	GATC	6 cut(s)	240, 381, 812, 925, 1042, 1150
BstMAI	GTCTC	3 cut(s)	167, 655, 979
BstMBI	GATC	6 cut(s)	237, 378, 809, 922, 1039, 1147
BstMWI	GCNNNNNNNGC	9 cut(s)	79, 154, 307, 348, 410, 598, 664, 716, 1331
BstNI	CCWGG	4 cut(s)	522, 721, 1112, 1161
BstNSI	RCATGY	1 cut(s)	1394
BstSCI	CCNGG	4 cut(s)	520, 719, 1110, 1159
BstSFI	CTRYAG	3 cut(s)	167, 644, 879
BstSLI	GKGCMC	1 cut(s)	1327
BstUI	CGCG	1 cut(s)	790
BstV1I	GCAGC	7 cut(s)	69, 163, 610, 613, 630, 679, 1346
BstX2I	RGATCY	3 cut(s)	809, 1039, 1147
BstYI	RGATCY	3 cut(s)	809, 1039, 1147
Bsu15I	ATCGAT	1 cut(s)	381
Bsu36I	CCTNAGG	1 cut(s)	419
BsuI	GTATCC	1 cut(s)	15
BsuRI	GGCC	4 cut(s)	499, 520, 689, 1363
BsuTUI	ATCGAT	1 cut(s)	381
BtgZI	GCGATG	1 cut(s)	113
BtsCI	GGATG	3 cut(s)	120, 447, 985
BtsI	GCAGTG	3 cut(s)	636, 1246, 1325
BtsIMutI	CAGTG	6 cut(s)	167, 545, 636, 1222, 1246, 1325
BveI	ACCTGC	1 cut(s)	859
Cac8I	GCNNGC	4 cut(s)	353, 1332, 1365, 1392
CaiI	CAGNNNCTG	1 cut(s)	166
CfoI	GCGC	2 cut(s)	459, 790
Cfr13I	GGNCC	3 cut(s)	61, 280, 497
ClaI	ATCGAT	1 cut(s)	381
Csp6I	GTAC	3 cut(s)	609, 844, 1137
CviAII	CATG	5 cut(s)	871, 926, 1027, 1174, 1391
CviQI	GTAC	3 cut(s)	609, 844, 1137
DdeI	CTNAG	5 cut(s)	419, 468, 548, 654, 715
DpnI	GATC	6 cut(s)	239, 380, 811, 924, 1041, 1149
DpnII	GATC	6 cut(s)	237, 378, 809, 922, 1039, 1147
DriI	GACNNNNNGTC	1 cut(s)	223
EaeI	YGGCCR	2 cut(s)	687, 1361
Eam1104I	CTCTTC	2 cut(s)	554, 763
Eam1105I	GACNNNNNGTC	1 cut(s)	223
EarI	CTCTTC	2 cut(s)	554, 763
EciI	GGCGGA	1 cut(s)	338
Eco130I	CCWWGG	1 cut(s)	690
Eco147I	AGGCCT	1 cut(s)	520
Eco31I	GGTCTC	1 cut(s)	979
Eco32I	GATATC	1 cut(s)	1309
Eco47I	GGWCC	2 cut(s)	61, 280
Eco57I	CTGAAG	3 cut(s)	143, 528, 1056
Eco81I	CCTNAGG	1 cut(s)	419
Eco88I	CYCGRG	1 cut(s)	1091
EcoO109I	RGGNCCY	1 cut(s)	280
EcoRII	CCWGG	4 cut(s)	520, 719, 1110, 1159
EcoRV	GATATC	1 cut(s)	1309
EcoT14I	CCWWGG	1 cut(s)	690
ErhI	CCWWGG	1 cut(s)	690
FaeI	CATG	5 cut(s)	874, 929, 1030, 1177, 1394
FalI	AAGNNNNNCTT	2 cut(s)	1029, 1061
FaqI	GGGAC	3 cut(s)	293, 869, 1328
FatI	CATG	5 cut(s)	870, 925, 1026, 1173, 1390
FauI	CCCGC	1 cut(s)	83
FauNDI	CATATG	1 cut(s)	621
FbaI	TGATCA	1 cut(s)	237
FokI	GGATG	3 cut(s)	127, 434, 992
FspBI	CTAG	3 cut(s)	803, 986, 1236
GlaI	GCGC	2 cut(s)	458, 789
GsaI	CCCAGC	1 cut(s)	710
GsuI	CTGGAG	2 cut(s)	322, 903
HaeIII	GGCC	4 cut(s)	499, 520, 689, 1363
HapII	CCGG	1 cut(s)	179
HhaI	GCGC	2 cut(s)	459, 790
Hin1II	CATG	5 cut(s)	874, 929, 1030, 1177, 1394
Hin6I	GCGC	2 cut(s)	457, 788
HinP1I	GCGC	2 cut(s)	457, 788
HincII	GTYRAC	1 cut(s)	586
HindII	GTYRAC	1 cut(s)	586
HindIII	AAGCTT	2 cut(s)	265, 411
HinfI	GANTC	6 cut(s)	175, 525, 542, 562, 815, 1373
HpaII	CCGG	1 cut(s)	179
HphI	GGTGA	3 cut(s)	545, 608, 1022
Hpy166II	GTNNAC	5 cut(s)	217, 586, 616, 800, 1325
Hpy188I	TCNGA	4 cut(s)	9, 315, 814, 979
Hpy188III	TCNNGA	5 cut(s)	418, 451, 920, 1091, 1400
Hpy8I	GTNNAC	5 cut(s)	217, 586, 616, 800, 1325
Hpy99I	CGWCG	1 cut(s)	229
HpyAV	CCTTC	7 cut(s)	104, 510, 510, 524, 821, 1007, 1120
HpyCH4III	ACNGT	3 cut(s)	137, 613, 1268
HpyCH4IV	ACGT	2 cut(s)	41, 203
HpyF10VI	GCNNNNNNNGC	9 cut(s)	79, 154, 307, 348, 410, 598, 664, 716, 1331
HpyF3I	CTNAG	5 cut(s)	419, 468, 548, 654, 715
HpySE526I	ACGT	2 cut(s)	41, 203
Hsp92II	CATG	5 cut(s)	874, 929, 1030, 1177, 1394
HspAI	GCGC	2 cut(s)	457, 788
KflI	GGGWCCC	1 cut(s)	280
Ksp22I	TGATCA	1 cut(s)	237
Kzo9I	GATC	6 cut(s)	237, 378, 809, 922, 1039, 1147
LguI	GCTCTTC	1 cut(s)	763
LmnI	GCTCC	1 cut(s)	486
Lsp1109I	GCAGC	7 cut(s)	69, 163, 610, 613, 630, 679, 1346
LweI	GCATC	2 cut(s)	1028, 1201
MaeI	CTAG	3 cut(s)	803, 986, 1236
MaeII	ACGT	2 cut(s)	41, 203
MaeIII	GTNAC	4 cut(s)	543, 991, 1030, 1066
MalI	GATC	6 cut(s)	239, 380, 811, 924, 1041, 1149
MboI	GATC	6 cut(s)	237, 378, 809, 922, 1039, 1147
MboII	GAAGA	6 cut(s)	273, 454, 571, 745, 750, 1049
MfeI	CAATTG	1 cut(s)	56
MflI	RGATCY	3 cut(s)	809, 1039, 1147
MhlI	GDGCHC	1 cut(s)	1327
MlsI	TGGCCA	2 cut(s)	689, 1363
MluCI	AATT	7 cut(s)	45, 56, 316, 331, 776, 834, 941
MluNI	TGGCCA	2 cut(s)	689, 1363
MlyI	GAGTC	4 cut(s)	169, 519, 551, 571
Mox20I	TGGCCA	2 cut(s)	689, 1363
MscI	TGGCCA	2 cut(s)	689, 1363
MseI	TTAA	6 cut(s)	357, 569, 783, 968, 1086, 1179
Msp20I	TGGCCA	2 cut(s)	689, 1363
MspA1I	CMGCKG	2 cut(s)	82, 1367
MspI	CCGG	1 cut(s)	179
MspR9I	CCNGG	4 cut(s)	522, 721, 1112, 1161
MunI	CAATTG	1 cut(s)	56
Mva1269I	GAATGC	3 cut(s)	710, 1293, 1379
MvaI	CCWGG	4 cut(s)	522, 721, 1112, 1161
MvnI	CGCG	1 cut(s)	790
MwoI	GCNNNNNNNGC	9 cut(s)	79, 154, 307, 348, 410, 598, 664, 716, 1331
NdeI	CATATG	1 cut(s)	621
NdeII	GATC	6 cut(s)	237, 378, 809, 922, 1039, 1147
NlaIII	CATG	5 cut(s)	874, 929, 1030, 1177, 1394
NlaIV	GGNNCC	5 cut(s)	63, 183, 281, 282, 554
NmeAIII	GCCGAG	1 cut(s)	66
NmuCI	GTSAC	2 cut(s)	543, 1066
NspI	RCATGY	1 cut(s)	1394
PaeI	GCATGC	1 cut(s)	1394
PceI	AGGCCT	1 cut(s)	520
PciSI	GCTCTTC	1 cut(s)	763
PctI	GAATGC	3 cut(s)	710, 1293, 1379
PfeI	GAWTC	2 cut(s)	815, 1373
PflMI	CCANNNNNTGG	1 cut(s)	926
PfoI	TCCNGGA	1 cut(s)	1110
PleI	GAGTC	4 cut(s)	169, 519, 550, 570
PpsI	GAGTC	4 cut(s)	169, 519, 550, 570
PpuMI	RGGWCCY	1 cut(s)	280
Psp5II	RGGWCCY	1 cut(s)	280
Psp6I	CCWGG	4 cut(s)	520, 719, 1110, 1159
PspFI	CCCAGC	1 cut(s)	706
PspGI	CCWGG	4 cut(s)	520, 719, 1110, 1159
PspN4I	GGNNCC	5 cut(s)	63, 183, 281, 282, 554
PspPI	GGNCC	3 cut(s)	61, 280, 497
PspPPI	RGGWCCY	1 cut(s)	280
PsrI	GAACNNNNNNTAC	4 cut(s)	885, 917, 1216, 1248
PstI	CTGCAG	3 cut(s)	171, 648, 883
PstNI	CAGNNNCTG	1 cut(s)	166
PsuI	RGATCY	3 cut(s)	809, 1039, 1147
PvuII	CAGCTG	1 cut(s)	1367
RsaI	GTAC	3 cut(s)	610, 845, 1138
RsaNI	GTAC	3 cut(s)	609, 844, 1137
SapI	GCTCTTC	1 cut(s)	763
SaqAI	TTAA	6 cut(s)	357, 569, 783, 968, 1086, 1179
Sau3AI	GATC	6 cut(s)	237, 378, 809, 922, 1039, 1147
Sau96I	GGNCC	3 cut(s)	61, 280, 497
ScaI	AGTACT	1 cut(s)	1138
SchI	GAGTC	4 cut(s)	169, 519, 551, 571
ScrFI	CCNGG	4 cut(s)	522, 721, 1112, 1161
SduI	GDGCHC	1 cut(s)	1327
SfaNI	GCATC	2 cut(s)	1028, 1201
SfcI	CTRYAG	3 cut(s)	167, 644, 879
SinI	GGWCC	2 cut(s)	61, 280
SphI	GCATGC	1 cut(s)	1394
Sse9I	AATT	7 cut(s)	45, 56, 316, 331, 776, 834, 941
SseBI	AGGCCT	1 cut(s)	520
SsiI	CCGC	6 cut(s)	80, 90, 149, 155, 185, 349
SspI	AATATT	1 cut(s)	952
SspMI	CTAG	3 cut(s)	803, 986, 1236
StuI	AGGCCT	1 cut(s)	520
StyD4I	CCNGG	4 cut(s)	520, 719, 1110, 1159
StyI	CCWWGG	1 cut(s)	690
TaaI	ACNGT	3 cut(s)	137, 613, 1268
TaiI	ACGT	2 cut(s)	44, 206
TaqI	TCGA	4 cut(s)	296, 381, 528, 1383
TasI	AATT	7 cut(s)	45, 56, 316, 331, 776, 834, 941
TatI	WGTACW	1 cut(s)	1136
TauI	GCSGC	4 cut(s)	82, 151, 157, 187
TfiI	GAWTC	2 cut(s)	815, 1373
Tru1I	TTAA	6 cut(s)	357, 569, 783, 968, 1086, 1179
Tru9I	TTAA	6 cut(s)	357, 569, 783, 968, 1086, 1179
TscAI	CASTG	6 cut(s)	167, 552, 636, 1222, 1246, 1332
TseFI	GTSAC	2 cut(s)	543, 1066
TseI	GCWGC	7 cut(s)	82, 151, 598, 601, 643, 667, 1334
Tsp45I	GTSAC	2 cut(s)	543, 1066
TspDTI	ATGAA	6 cut(s)	423, 638, 996, 1139, 1386, 1419
TspGWI	ACGGA	1 cut(s)	1311
TspRI	CASTG	6 cut(s)	167, 552, 636, 1222, 1246, 1332
Van91I	CCANNNNNTGG	1 cut(s)	926
VneI	GTGCAC	1 cut(s)	1323
VpaK11BI	GGWCC	2 cut(s)	61, 280
XapI	RAATTY	2 cut(s)	316, 776
XceI	RCATGY	1 cut(s)	1394
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1191
XspI	CTAG	3 cut(s)	803, 986, 1236
ZrmI	AGTACT	1 cut(s)	1138

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.